Muhammad Ovais
MPhil Research Scholar (Nanobiotechnology)
Supervisors: Dr.Zabta Khan Shinwari
Phone: +923037622664
Address: Department of Biotechnology, Quaid-i-Azam University Islamabad
Supervisors: Dr.Zabta Khan Shinwari
Phone: +923037622664
Address: Department of Biotechnology, Quaid-i-Azam University Islamabad
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Papers by Muhammad Ovais
in this study. A case-control study was conducted in Pashtun population of Khyber Pakhtunkhwa province of
Pakistan in which 200 hospital based oral cancer cases and 151 population based healthy controls exposed to
similar environmental conditions were included. Sociodemographic data were obtained and blood samples were
collected with informed consent for analysis. GSTM1 and GSTT1 were analysed through conventional PCR
method while specific RT-PCR method was used to detect CYP1A1 polymorphisms. Results were analyzed for
conditional logistic regression model by SPSS version 20. The study shows that patients with either GSTM1 or
GSTT1 null genotypes have significantly higher risk of oral cancer (adjusted odds (OR): (3.019 (1.861-4.898)
and 3.011(1.865-4.862), respectively), which further increased when either one or both null genes were present in
combination (adjusted odds (OR): (3.627 (1.981-6.642 and 9.261 (4.495-19.079), respectively). CYP1A1 rs4646903
gene variants individually showed weak association OR: 1.121 (0.717-1.752); however, in the presence of GSTM1
and/or GSTT1 null genotypes further increasing the association (adjusted odds (ORs): 4.576 (2.038-10.273), 5.593
(2.530-12.362) and 16.10 (3.854-67.260 for GSTM/GSTT null and CYP1A1 wild type, GSTM/GSTT either null
and CYP1A1 variant alleles, and all 3 gene polymorphisms combinations, respectively). Our findings suggest
that presence of GSTM1 and/or GSTT1 null genotypes along
in this study. A case-control study was conducted in Pashtun population of Khyber Pakhtunkhwa province of
Pakistan in which 200 hospital based oral cancer cases and 151 population based healthy controls exposed to
similar environmental conditions were included. Sociodemographic data were obtained and blood samples were
collected with informed consent for analysis. GSTM1 and GSTT1 were analysed through conventional PCR
method while specific RT-PCR method was used to detect CYP1A1 polymorphisms. Results were analyzed for
conditional logistic regression model by SPSS version 20. The study shows that patients with either GSTM1 or
GSTT1 null genotypes have significantly higher risk of oral cancer (adjusted odds (OR): (3.019 (1.861-4.898)
and 3.011(1.865-4.862), respectively), which further increased when either one or both null genes were present in
combination (adjusted odds (OR): (3.627 (1.981-6.642 and 9.261 (4.495-19.079), respectively). CYP1A1 rs4646903
gene variants individually showed weak association OR: 1.121 (0.717-1.752); however, in the presence of GSTM1
and/or GSTT1 null genotypes further increasing the association (adjusted odds (ORs): 4.576 (2.038-10.273), 5.593
(2.530-12.362) and 16.10 (3.854-67.260 for GSTM/GSTT null and CYP1A1 wild type, GSTM/GSTT either null
and CYP1A1 variant alleles, and all 3 gene polymorphisms combinations, respectively). Our findings suggest
that presence of GSTM1 and/or GSTT1 null genotypes along