Abstract
A combination of an enzyme-labeled antibody release coating and a novel fluorescent enzyme substrate-copolymerized hydrogel in a microchannel for a single-step, no-wash microfluidic immunoassay is demonstrated. This hydrogel discriminates the free enzyme-conjugated antibody from an antigen-enzyme-conjugated antibody immunocomplex based on the difference in molecular size. A selective and sensitive immunoassay, with 10-1000 ng mL(-1) linear range, is reported.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkaline Phosphatase / chemistry
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Alkaline Phosphatase / metabolism
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Antibodies / chemistry
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Antibodies / immunology*
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Antigens / chemistry
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Antigens / immunology
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Antigens / metabolism
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Coumarins / chemistry
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Enzymes / chemistry*
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Enzymes / metabolism
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Enzymes, Immobilized / chemistry
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Enzymes, Immobilized / metabolism
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Fluorescent Dyes / chemistry*
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Humans
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Hydrogel, Polyethylene Glycol Dimethacrylate / chemistry*
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Immunoassay / instrumentation
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Immunoassay / methods*
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Microfluidic Analytical Techniques* / instrumentation
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Polymerization
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Substrate Specificity
Substances
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Antibodies
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Antigens
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Coumarins
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Enzymes
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Enzymes, Immobilized
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Fluorescent Dyes
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Hydrogel, Polyethylene Glycol Dimethacrylate
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Alkaline Phosphatase