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Massively parallel pyrosequencing in HIV research

AIDS. 2008 Jul 31;22(12):1411-5. doi: 10.1097/QAD.0b013e3282fc972e.

Abstract

The new massively parallel sequencing methods are so astonishing one wonders whether space aliens are secretly behind them. One technician, running a single instrument, can obtain up to ~1 billion bases of DNA sequence in a few days. Here we describe the new sequencing methods, briefly present a few applications in HIV research, and then speculate on future directions.

Publication types

  • Review

MeSH terms

  • AIDS-Related Opportunistic Infections / diagnosis
  • DNA, Viral / analysis*
  • Genetic Variation
  • HIV / genetics*
  • Humans
  • Sequence Analysis, DNA / methods*
  • Virus Integration / genetics

Substances

  • DNA, Viral