Abstract
In Drosophila melanogaster, Dicer-2/R2D2 and Dicer-1 generate small interfering RNA (siRNA) and microRNA (miRNA), respectively. Here we identify a novel dsRNA-binding protein, which we named R3D1-L, that forms a stable complex with Dicer-1 in vitro and in vivo. While depletion of R3D1-L by RNAi causes accumulation of precursor miRNA (pre-miRNA) in S2 cells, recombinant R3D1-L enhances miRNA production by Dicer-1 in vitro. Furthermore, R3D1 deficiency causes miRNA-generating defect and severe sterility in male and female flies. Therefore, R3D1-L functions in concert with Dicer-1 in miRNA biogenesis and is required for reproductive development in Drosophila.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adenosine Triphosphate / metabolism
-
Amino Acid Sequence
-
Animals
-
Base Sequence
-
Drosophila Proteins / genetics
-
Drosophila Proteins / metabolism*
-
Drosophila melanogaster / genetics*
-
Drosophila melanogaster / metabolism*
-
Female
-
In Vitro Techniques
-
Infertility / genetics
-
Infertility / metabolism
-
Male
-
MicroRNAs / genetics
-
MicroRNAs / metabolism*
-
Molecular Sequence Data
-
Mutagenesis, Insertional
-
RNA Helicases / genetics
-
RNA Helicases / metabolism
-
RNA Interference
-
RNA Processing, Post-Transcriptional
-
RNA-Binding Proteins / genetics
-
RNA-Binding Proteins / metabolism*
-
Recombinant Proteins / genetics
-
Recombinant Proteins / metabolism
-
Ribonuclease III / metabolism*
-
Sequence Homology, Amino Acid
-
Substrate Specificity
Substances
-
Drosophila Proteins
-
Loqs protein, Drosophila
-
MicroRNAs
-
RNA-Binding Proteins
-
Recombinant Proteins
-
Adenosine Triphosphate
-
DCR-2 protein, Drosophila
-
Ribonuclease III
-
RNA Helicases