WO2024114467A1

WO2024114467A1 - Shp2 inhibitors, compositions and methods thereof

Info

Publication number: WO2024114467A1
Application number: PCT/CN2023/133123
Authority: WO
Inventors: Ninghui YU; Rongliang Lou
Original assignee: Canwell Biotech Limited
Priority date: 2022-11-30
Filing date: 2023-11-22
Publication date: 2024-06-06
Also published as: WO2024113194A1

Abstract

The invention provides novel phosphine oxide compounds that inhibit SHP2,compositions thereof, as well as methods of preparation thereof and use for treating diseases associated with SHP2 (e.g., cancers).

Description

SHP2 INHIBITORS, COMPOSITIONS AND METHODS THEREOF

Priority Claims and Related Applications

This application claims the benefit of priority to PCT Int’l Application No. PCT/CN2022/135205, filed November 30, 2022, the entire content of which is incorporated herein by reference for all purposes.

Technical Field of the Invention

This invention relates to certain novel SHP2 inhibitors, which are useful for treating SHP2 mediated diseases. More specifically, this disclosure is directed to phosphine oxide compounds which inhibit SHP2 and compositions comprising these compounds, methods of treating diseases associated with SHP2, and methods of synthesizing these compounds.

Background of the Invention

Src homology region 2 (SH2) -containing protein tyrosine phosphatase 2 (SHP2) , encoded by the PTPN11 gene, is a cytoplasmic non-receptor phosphotyrosine phosphatase (Blood 2011; 118: 1504-1515. Nature 2013; 499: 491-495) . SHP2 positively modulates RAS signaling and plays roles in cell proliferation, migration, differentiation and other important physiological processes (Mol Cell 2004; 13: 341-355) . Structurally, SHP2 contains a protein tyrosine phosphatase catalytic domain (PTP) , C-terminal tail with tyrosyl phosphorylation sites, and two SH2 domains, N-SH2 and C-SH2 domain. The N-SH2 domain plays a key role in de-phosphorylating SHP2 itself with two non-overlapping ligand binding sites, while the C-SH2 domain provides binding energy and specificity (J. Cell. Mol. Med 2015; 19: 2075-2083) . Both N-and C-SH2 domains control the SHP2 subcellular localization and function. The PTP domain has a P ring catalytic structure to de-phosphorylate substrates (J. Biol. Chem 2013; 15: 10472-10482) .

The SHP2 has two types of mutations, loss-of-function (LOF) mutations and gain-of-function (GOF) mutations. The GOF mutations of SHP2 mainly occur in cancers and promote cancer progression in cell-autonomous and non-autonomous mechanisms.

The GOF mutants of SHP2 include D61G (Med. Sci. Monit 2017; 23: 2931-2938) , D61Y (Exp. Hematol 2008; 36: 1285-1296) , E76K (Mol. Carcinog 2018; 57: 619-628) , E76Q (J Chem Inf Model 2019; 59: 3229-3239) and T507K (J. Biol. Chem 2020; 18: 6187-6201) , which were observed in juvenile myelomonocytic leukemia, colorectal cancer, glioblastoma multiforme and other diseases.

SHP2^E76K, a GOF mutation, activates Erk and Src to promote progression of lung cancer. SHP2^E76K can also promote the malignance of glioblastoma multiform cells through Erk/cAMP/CREB signaling pathway (OncoTargets Ther 2019; 12: 9435-9447. Carcinogenesis 2014; 8: 1717-1725) . Overall, SHP2 represents a potential biomarker and therapeutic target in several types of human cancer.

While SHP2 inhibitors have been reported (e.g., WO 2021/088945; WO 2020/094104; WO 2020/0210384; WO 2020/081848; WO 2019/182960; WO 2019/118909; WO 2018/172984; WO 2018/136265; WO 2018/136264; WO 2018/081091; WO 2018/057884; WO 2018/013597; WO 2017/216706; WO 2017/211303; WO 2017/210134; WO 2016/203406; WO 2016/203405; WO 2016/203404; WO 2015/107495; WO 2015/107494; and WO 2015/107493) , , significant challenges remain in finding and developing a compound the safety and efficacy required for regulatory approval.

Thus, an urgent and unmet need exists for novel SHP2 inhibitors with improved potency and selectivity, good pharmacokinetics and safety profiles suitable for development into an approved medicine.

Summary of the Invention

The present invention provides novel phosphine oxide compounds that are SHP2 inhibitors and have improved superior potency and selectivity profiles as well as good pharmacokinetics characteristics. The invention further provides pharmaceutical compositions and methods of preparation and use of these compounds in treating a variety of diseases and conditions, such as SHP2-mediated diseases.

In one aspect, the invention generally relates to a compound having the structural formula of (I) :

wherein

X is S or a single bond;

Y¹ is CR⁷ or N;

Y² is CR⁸ or N, provided that if one of Y¹ and Y² is N, the other is not N;

Ring A is a unsubstituted or substituted, 5-or 6-membered heterocyclic ring or a 7-to 14-membered bi-or tri-cyclic spiro or fused heterocyclic ring system;

R³ is H, R, NH₂ or NRR';

R⁴ is H, CH₂OH, C (O) OH, C (O) OR, C (O) NH₂, C (O) NHR or C (O) NRR’;

R⁵ is H, F, Cl or CF₃;

each of R⁶, R⁷ and R⁸ is independently H, F, CN, Cl, OH, NH₂, NRR’, R, OR, C (O) NH₂, C (O) NRR’, triazolyl, or P (O) RR’, provided that one of R⁶, R⁷ and R⁸ is P (O) RR’; and

each of R and R’ is independently a C_1-6 alkyl, cyclopropyl, cyclobutyl or cyclopentyl; or, R and R’ together with the N atom they are bonded to form a 5-membered heterocyclic ring, or a pharmaceutically acceptable form or an isotope derivative thereof.

In another aspect, the invention generally relates to a pharmaceutical composition comprising a compound disclosed herein and a pharmaceutically acceptable excipient, carrier, or diluent.

In yet another aspect, the invention generally relates to a pharmaceutical composition comprising a compound of the invention effective to treat or reduce cancer, or a related disease or condition.

In yet another aspect, the invention generally relates to a unit dosage form comprising a pharmaceutical composition disclosed herein.

In yet another aspect, the invention generally relates to a method for treating or reducing a disease or condition, comprising administering to a subject in need thereof a therapeutically effective amount of a pharmaceutical composition comprising a compound disclosed herein and a pharmaceutically acceptable excipient, carrier, or diluent.

In yet another aspect, the invention generally relates to use of a compound disclosed herein for treating or reducing a disease or condition.

In yet another aspect, the invention generally relates to use of a compound disclosed herein and a pharmaceutically acceptable excipient, carrier, or diluent, in preparation of a medicament for treating or reducing a disease or condition.

Definitions

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. General principles of organic chemistry, as well as specific functional moieties and reactivity, are described in “Organic Chemistry” , Thomas Sorrell, University Science Books, Sausalito: 1999, and “March’s Advanced Organic Chemistry” , 5^th Ed.: Smith, M.B. and March, J., John Wiley & Sons, New York: 2001, the entire contents of which are hereby incorporated by reference.

Certain compounds of the present invention may exist in particular geometric or stereoisomeric forms. The present invention contemplates all such compounds, including cis-and trans-isomers, R-and S-enantiomers, diastereomers, (D) -isomers, (L) -isomers, the racemic mixtures thereof, and other mixtures thereof, as falling within the scope of the invention. Additional asymmetric carbon atoms may be present in a substituent such as an alkyl group. All such isomers, as well as mixtures thereof, are intended to be included in this invention.

Isomeric mixtures containing any of a variety of isomer ratios may be utilized in accordance with the present invention. For example, where only two isomers are combined, mixtures containing 50: 50, 60: 40, 70: 30, 80: 20, 90: 10, 95: 5, 96: 4, 97: 3, 98: 2, 99: 1, or 100: 0 isomer ratios are contemplated by the present invention. Those of ordinary skill in the art will readily appreciate that analogous ratios are contemplated for more complex isomer mixtures.

If, for instance, a particular enantiomer of a compound of the present invention is desired, it may be prepared by asymmetric synthesis, or by derivation with a chiral auxiliary, where the resulting diastereomeric mixture is separated and the auxiliary group cleaved to provide the pure desired enantiomers. Alternatively, where the molecule contains a basic functional group, such as amino, or an acidic functional group, such as carboxyl, diastereomeric salts are formed with an appropriate optically-active acid or base, followed by resolution of the diastereomers thus formed by fractional crystallization or chromatographic methods well known in the art, and subsequent recovery of the pure enantiomers.

Solvates and polymorphs of the compounds of the invention are also contemplated herein. Solvates of the compounds of the present invention include, for example, hydrates.

Definitions of specific functional groups and chemical terms are described in more detail below. When a range of values is listed, it is intended to encompass each value and sub-range within the range. For example, "C_1-6 alkyl" is intended to encompass, C₁, C₂, C₃, C₄, C₅, C₆, C_1-6, C_1-5, C_1-4, C_1-3, C_1-2, C_2-6, C_2-5, C_2-4, C_2-3, C_3-6, C_3-5, C_3-4, C_4-6, C_4-5, and C_5-6 alkyl.

As used herein, the term "alkyl" refers to a straight, branched or cyclic hydrocarbon radical consisting solely of carbon and hydrogen atoms, containing no unsaturation, having from one to ten carbon atoms (e.g., C_1-10 alkyl) . Whenever it appears herein, a numerical range such as "1 to 10" refers to each integer in the given range; e.g., "1 to 10 carbon atoms" means that the alkyl group can consist of 1 carbon atom, 2 carbon atoms, 3 carbon atoms, etc., up to and including 10 carbon atoms, although the present definition also covers the occurrence of the term "alkyl" where no numerical range is designated. In some embodiments, “alkyl” can be a C_1-6 alkyl group. In some embodiments, alkyl groups have 1 to 10, 1 to 8, 1 to 6, or 1 to 3 carbon atoms.

Representative saturated straight chain alkyls include, but are not limited to, -methyl, -ethyl, -n-propyl, -n-butyl, -n-pentyl, and -n-hexyl; while saturated branched alkyls include, but are not limited to, -isopropyl, -sec-butyl, -isobutyl, -tert-butyl, -isopentyl, 2-methylbutyl, 3-methylbutyl, 2-methylpentyl, 3-methylpentyl, 4-methylpentyl, 2-methylhexyl, 3-methylhexyl, 4-methylhexyl, 5-methylhexyl, 2, 3-dimethylbutyl, and the like. The alkyl is attached to the parent molecule by a single bond.

Unless stated otherwise in the specification, an alkyl group is optionally substituted by one or more of substituents.

As used herein, the term "inhibit" refers to any measurable reduction of biological activity. Thus, as used herein, "inhibit" or "inhibition" may be referred to as a percentage of a normal level of activity.

As used herein, the term “effective amount” or “therapeutically effective amount” of an active agent refers to an amount sufficient to elicit the desired biological response. The effective amount, when administered in a proper dosing regimen, is sufficient to reduce or ameliorate the severity, duration or progression of the disorder being treated, prevent the advancement of the disorder being treated, cause the regression of the disorder being treated, or enhance or improve the prophylactic or therapeutic effect (s) of another therapy. As will be appreciated by those of ordinary skill in this art, the effective amount of a compound of the invention may vary depending on such factors as the desired biological endpoint, the pharmacokinetics of the compound, the disease being treated, the mode of administration, and the patient. An effective amount can be readily determined by a skilled physician, e.g., by first administering a low dose of the pharmacological agent (s) and then incrementally increasing the dose until the desired therapeutic effect is achieved with minimal or no undesirable side effects.

As used herein, the terms “treatment” or “treating” a disease or disorder refers to a method of reducing, delaying or ameliorating such a condition before or after it has occurred. Treatment may be directed at one or more effects or symptoms of a disease and/or the underlying pathology. The treatment can be any reduction and can be, but is not limited to, the complete ablation of the disease or the symptoms of the disease. As compared with an equivalent untreated control, such reduction or degree of prevention is at least 5%, 10%, 20%, 40%, 50%, 60%, 80%, 90%, 95%, or 100%as measured by any standard technique.

As used herein, the terms “prevent” , “preventing” , or “prevention” refer to a method for precluding, delaying, averting, or stopping the onset, incidence, severity, or recurrence of a disease or condition. For example, a method is considered to be a prevention if there is a reduction or delay in onset, incidence, severity, or recurrence of a disease or condition or one or more symptoms thereof in a subject susceptible to the disease or condition as compared to a subject not receiving the method. The disclosed method is also considered to be a prevention if there is a reduction or delay in onset, incidence, severity, or recurrence of osteoporosis or one or more symptoms of a disease or condition in a subject susceptible to the disease or condition after receiving the method as compared to the subject's progression prior to receiving treatment. Thus, the reduction or delay in onset, incidence, severity, or recurrence of osteoporosis can be about a 5, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100%, or any amount of reduction in between.

As used herein, a "pharmaceutically acceptable form" of a disclosed compound includes, but is not limited to, pharmaceutically acceptable salts, esters, hydrates, solvates, polymorphs, isomers, prodrugs, and isotopically labeled derivatives thereof. In one embodiment, a "pharmaceutically acceptable form" includes, but is not limited to, pharmaceutically acceptable salts, esters, prodrugs and isotopically labeled derivatives thereof. In some embodiments, a "pharmaceutically acceptable form" includes, but is not limited to, pharmaceutically acceptable isomers and stereoisomers, prodrugs and isotopically labeled derivatives thereof.

In certain embodiments, the pharmaceutically acceptable form is a pharmaceutically acceptable salt. As used herein, the term "pharmaceutically acceptable salt" refers to those salts which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of subjects without undue toxicity, irritation, allergic response and the like, and are commensurate with a reasonable benefit/risk ratio. Pharmaceutically acceptable salts are well known in the art. For example, Berge et al. describes pharmaceutically acceptable salts in detail in J. Pharmaceutical Sciences (1977) 66: 1-19. Pharmaceutically acceptable salts of the compounds provided herein include those derived from suitable inorganic and organic acids and bases. Examples of pharmaceutically acceptable, nontoxic acid addition salts are salts of an amino group formed with inorganic acids such as hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid and perchioric acid or with organic acids such as acetic acid, oxalic acid, maleic acid, tartaric acid, citric acid, succinic acid or malonic acid or by using other methods used in the art such as ion exchange. Other pharmaceutically acceptable salts include adipate, alginate, ascorbate, aspartate, benzenesulfonate, besylate, benzoate, bisulfate, borate, butyrate, camphorate, camphorsulfonate, citrate, cyclopentanepropionate, digluconate, dodecylsulfate, ethanesulfonate, formate, fumarate, glucoheptonate, glycerophosphate, gluconate, hemisulfate, heptanoate, hexanoate, hydroiodide, 2-hydroxy-ethanesulfonate, lactobionate, lactate, laurate, lauryl sulfate, malate, maleate, malonate, methanesulfonate, 2-naphthalenesulfonate, nicotinate, nitrate, oleate, oxalate, palmitate, pamoate, pectinate, persulfate, 3-phenylpropionate, phosphate, picrate, pivalate, propionate, stearate, succinate, sulfate, tartrate, thiocyanate, p-toluenesulfonate, undecanoate, valerate salts, and the like. In some embodiments, organic acids from which salts can be derived include, for example, acetic acid, propionic acid, glycolic acid, pyruvic acid, oxalic acid, lactic acid, trifluoracetic acid, maleic acid, malonic acid, succinic acid, fumaric acid, tartaric acid, citric acid, benzoic acid, cinnamic acid, mandelic acid, methanesulfonic acid, ethanesulfonic acid, p-toluenesulfonic acid, salicylic acid, and the like.

The salts can be prepared in situ during the isolation and purification of the disclosed compounds, or separately, such as by reacting the free base or free acid of a parent compound with a suitable base or acid, respectively. Pharmaceutically acceptable salts derived from appropriate bases include alkali metal, alkaline earth metal, ammonium and N⁺ (C_1-4alkyl) ₄ salts. Representative alkali or alkaline earth metal salts include sodium, lithium, potassium, calcium, magnesium, iron, zinc, copper, manganese, aluminum, and the like. Further pharmaceutically acceptable salts include, when appropriate, nontoxic ammonium, quaternary ammonium, and amine cations formed using counterions such as halide, hydroxide, carboxylate, sulfate, phosphate, nitrate, lower alkyl sulfonate and aryl sulfonate. Organic bases from which salts can be derived include, for example, primary, secondary, and tertiary amines, substituted amines, including naturally occurring substituted amines, cyclic amines, basic ion exchange resins, and the like, such as isopropylamine, trimethylamine, diethylamine, triethylamine, tripropylamine, and ethanolamine. In some embodiments, the pharmaceutically acceptable base addition salt can be chosen from ammonium, potassium, sodium, calcium, and magnesium salts.

In certain embodiments, the pharmaceutically acceptable form is a "solvate" (e.g., a hydrate) . As used herein, the term "solvate" refers to compounds that further include a stoichiometric or non-stoichiometric amount of solvent bound by non-covalent intermolecular forces. The solvate can be of a disclosed compound or a pharmaceutically acceptable salt thereof. Where the solvent is water, the solvate is a "hydrate" . Pharmaceutically acceptable solvates and hydrates are complexes that, for example, can include 1 to about 100, or 1 to about 10, or 1 to about 2, about 3 or about 4, solvent or water molecules. It will be understood that the term "compound" as used herein encompasses the compound and solvates of the compound, as well as mixtures thereof.

In certain embodiments, the pharmaceutically acceptable form is a prodrug. As used herein, the term "prodrug" (or “pro-drug” ) refers to compounds that are transformed in vivo to yield a disclosed compound or a pharmaceutically acceptable form of the compound. A prodrug can be inactive when administered to a subject, but is converted in vivo to an active compound, for example, by hydrolysis (e.g., hydrolysis in blood) . In certain cases, a prodrug has improved physical and/or delivery properties over the parent compound. Prodrugs can increase the bioavailability of the compound when administered to a subject (e.g., by permitting enhanced absorption into the blood following oral administration) or which enhance delivery to a biological compartment of interest (e.g., the brain or lymphatic system) relative to the parent compound. Exemplary prodrugs include derivatives of a disclosed compound with enhanced aqueous solubility or active transport through the gut membrane, relative to the parent compound.

The prodrug compound often offers advantages of solubility, tissue compatibility or delayed release in a mammalian organism (see, e.g., Bundgard, H., Design of Prodrugs (1985) , pp. 7-9, 21-24 (Elsevier, Amsterdam) . A discussion of prodrugs is provided in Higuchi, T., et al., "Pro-drugs as Novel Delivery Systems, " A.C.S. Symposium Series, Vol. 14, and in Bioreversible Carriers in Drug Design, ed. Edward B. Roche, American Pharmaceutical Association and Pergamon Press, 1987, both of which are incorporated in full by reference herein. Exemplary advantages of a prodrug can include, but are not limited to, its physical properties, such as enhanced water solubility for parenteral administration at physiological pH compared to the parent compound, or it can enhance absorption from the digestive tract, or it can enhance drug stability for long-term storage.

Prodrugs commonly known in the art include well-known acid derivatives, such as, for example, esters prepared by reaction of the parent acids with a suitable alcohol, amides prepared by reaction of the parent acid compound with an amine, basic groups reacted to form an acylated base derivative, etc. Of course, other prodrug derivatives may be combined with other features disclosed herein to enhance bioavailability. As such, those of skill in the art will appreciate that certain of the presently disclosed compounds having free amino, arnido, hydroxy or carboxylic groups can be converted into prodrugs. Prodrugs include compounds having an amino acid residue, or a polypeptide chain of two or more (e.g., two, three or four) amino acid residues which are covalently joined through peptide bonds to free amino, hydroxy or carboxylic acid groups of the presently disclosed compounds. The amino acid residues include the 20 naturally occurring amino acids commonly designated by three letter symbols and also include 4-hydroxyproline, hydroxylysine, demosine, isodemosine, 3-methylhistidine, norvalin, beta-alanine, gamma-aminobutyric acid, citrulline homocysteine, homoserine, ornithine and methionine sulfone. Prodrugs also include compounds having a carbonate, carbamate, amide or alkyl ester moiety covalently bonded to any of the above substituents disclosed herein.

As used herein, the term “pharmaceutically acceptable” excipient, carrier, or diluent refers to a pharmaceutically acceptable material, composition or vehicle, such as a liquid or solid filler, diluent, excipient, solvent or encapsulating material, involved in carrying or transporting the subject pharmaceutical agent from one organ, or portion of the body, to another organ, or portion of the body. Each carrier must be "acceptable" in the sense of being compatible with the other ingredients of the formulation and not injurious to the patient. Some examples of materials which can serve as pharmaceutically-acceptable carriers include: sugars, such as lactose, glucose and sucrose; starches, such as corn starch and potato starch; cellulose, and its derivatives, such as sodium carboxymethyl cellulose, ethyl cellulose and cellulose acetate; powdered tragacanth; malt; gelatin; talc; excipients, such as cocoa butter and suppository waxes; oils, such as peanut oil, cottonseed oil, safflower oil, sesame oil, olive oil, corn oil and soybean oil; glycols, such as propylene glycol; polyols, such as glycerin, sorbitol, mannitol and polyethylene glycol; esters, such as ethyl oleate and ethyl laurate; agar; buffering agents, such as magnesium hydroxide and aluminum hydroxide; alginic acid; pyrogen-free water; isotonic saline; Ringer's solution; ethyl alcohol; phosphate buffer solutions; and other non-toxic compatible substances employed in pharmaceutical formulations. Wetting agents, emulsifiers and lubricants, such as sodium lauryl sulfate, magnesium stearate, and polyethylene oxide-polypropylene oxide copolymer as well as coloring agents, release agents, coating agents, sweetening, flavoring and perfuming agents, preservatives and antioxidants can also be present in the compositions.

As used herein, the term “subject” refers to any animal (e.g., a mammal) , including, but not limited to humans, non-human primates, rodents, and the like, which is to be the recipient of a particular treatment. Typically, the terms “subject” and “patient” are used interchangeably herein in reference to a human subject.

As used herein, the term “low dosage” refers to at least 5%less (e.g., at least 10%, 20%, 50%, 80%, 90%, or even 95%) than the lowest standard recommended dosage of a particular compound formulated for a given route of administration for treatment of any human disease or condition. For example, a low dosage of an agent that is formulated for administration by inhalation will differ from a low dosage of the same agent formulated for oral administration.

As used herein, the term “high dosage” is meant at least 5% (e.g., at least 10%, 20%, 50%, 100%, 200%, or even 300%) more than the highest standard recommended dosage of a particular compound for treatment of any human disease or condition.

Isotopically-labeled compounds are also within the scope of the present disclosure. As used herein, an "isotopically-labeled compound" or "isotope derivative" refers to a presently disclosed compound including pharmaceutical salts and prodrugs thereof, each as described herein, in which one or more atoms are replaced by an atom having an atomic mass or mass number different from the atomic mass or mass number usually found in nature. Examples of isotopes that can be incorporated into compounds presently disclosed include isotopes of hydrogen, carbon, nitrogen, oxygen, phosphorous, fluorine and chlorine, such as ²H, ³H, ¹³C, ¹⁴C, ¹⁵N, ¹⁸O, ¹⁷O, ³¹P, ³²P, ³⁵S, ¹⁸F, and ³⁶Cl, respectively.

By isotopically-labeling the presently disclosed compounds, the compounds may be useful in drug and/or substrate tissue distribution assays. Tritiated (³H) and carbon-14 (¹⁴C) labeled compounds are particularly preferred for their ease of preparation and detectability. Further, substitution with heavier isotopes such as deuterium (²H) can afford certain therapeutic advantages resulting from greater metabolic stability, for example increased in vivo half-life or reduced dosage requirements and, hence, may be preferred in some circumstances. Isotopically labeled compounds presently disclosed, including pharmaceutical salts, esters, and prodrugs thereof, can be prepared by any means known in the art. Benefits may also be obtained from replacement of normally abundant ¹²C with ¹³C. (See, WO 2007/005643, WO 2007/005644, WO 2007/016361, and WO 2007/016431. )

For example, deuterium (²H) can be incorporated into a compound disclosed herein for the purpose in order to manipulate the oxidative metabolism of the compound by way of the primary kinetic isotope effect. The primary kinetic isotope effect is a change of the rate for a chemical reaction that results from exchange of isotopic nuclei, which in turn is caused by the change in ground state energies necessary for covalent bond formation after this isotopic exchange. Exchange of a heavier isotope usually results in a lowering of the ground state energy for a chemical bond and thus causes a reduction in the rate in rate-limiting bond breakage. If the bond breakage occurs in or in the vicinity of a saddle-point region along the coordinate of a multi-product reaction, the product distribution ratios can be altered substantially. For explanation: if deuterium is bonded to a carbon atom at a non-exchangeable position, rate differences of k_M/k_D = 2-7 are typical. If this rate difference is successfully applied to a compound disclosed herein that is susceptible to oxidation, the profile of this compound in vivo can be drastically modified and result in improved pharmacokinetic properties.

When discovering and developing therapeutic agents, the person skilled in the art is able to optimize pharmacokinetic parameters while retaining desirable in vitro properties. It is reasonable to assume that many compounds with poor pharmacokinetic profiles are susceptible to oxidative metabolism. In vitro liver microsomal assays currently available provide valuable information on the course of oxidative metabolism of this type, which in turn permits the rational design of deuterated compounds of those disclosed herein with improved stability through resistance to such oxidative metabolism. Significant improvements in the pharmacokinetic profiles of compounds disclosed herein are thereby obtained, and can be expressed quantitatively in terms of increases in the in vivo half-life (t/2) , concen-tra-tion at maximum therapeutic effect (C_max) , area under the dose response curve (AUC) , and F; and in terms of reduced clearance, dose and materials costs.

The following is intended to illustrate the above: a compound which has multiple potential sites of attack for oxidative metabolism, for example benzylic hydrogen atoms and hydrogen atoms bonded to a nitrogen atom, is prepared as a series of analogues in which various combinations of hydrogen atoms are replaced by deuterium atoms, so that some, most or all of these hydrogen atoms have been replaced by deuterium atoms. Half-life determinations enable favorable and accurate determination of the extent of the extent to which the improvement in resistance to oxidative metabolism has improved. In this way, it is determined that the half-life of the parent compound can be extended by up to 100%as the result of deuterium-hydrogen exchange of this type.

Deuterium-hydrogen exchange in a compound disclosed herein can also be used to achieve a favorable modification of the metabolite spectrum of the starting compound in order to diminish or eliminate undesired toxic metabolites. For example, if a toxic metabolite arises through oxidative carbon-hydrogen (C-H) bond cleavage, it can reasonably be assumed that the deuterated analogue will greatly diminish or eliminate production of the unwanted metabolite, even if the particular oxidation is not a rate-determining step. Further information on the state of the art with respect to deuterium-hydrogen exchange may be found, for example in Hanzlik et al., J. Org. Chem. 55, 3992-3997, 1990, Reider et al., J. Org. Chem. 52, 3326-3334, 1987, Foster, Adv. Drug Res. 14, 1-40, 1985, Gillette et al, Biochemistry 33 (10) 2927-2937, 1994, and Jarman et al. Carcinogenesis 16 (4) , 683-688, 1993.

Compounds of the present invention are, subsequent to their preparation, preferably isolated and purified to obtain a composition containing an amount by weight equal to or greater than 95% ( “substantially pure” ) , which is then used or formulated as described herein. In certain embodiments, the compounds of the present invention are more than 99%pure.

Combinations of substituents and variables envisioned by this invention are only those that result in the formation of stable compounds. The term “stable” , as used herein, refers to compounds which possess stability sufficient to allow manufacture and which maintains the integrity of the compound for a sufficient period of time to be useful for the purposes detailed herein (e.g., therapeutic or prophylactic administration to a subject) .

The recitation of a listing of chemical groups in any definition of a variable herein includes definitions of that variable as any single group or combination of listed groups. The recitation of an embodiment for a variable herein includes that embodiment as any single embodiment or in combination with any other embodiments or portions thereof.

Detailed Description of the Invention

The invention is based in part on the unexpected discovery of novel phosphine oxide compounds that exhibit superior superior potency and selectivity profiles as SHP2 inhibitors and possess good pharmacokinetics characteristics. Also disclosed herein are pharmaceutical compositions and methods of preparation and use of these compounds in treating a variety of diseases and conditions, such as SHP2-mediated diseases.

wherein

X is S or a single bond;

Y¹ is CR⁷ or N;

R³ is H, R, NH₂ or NRR';

R⁴ is H, CH₂OH, C (O) OH, C (O) OR, C (O) NH₂, C (O) NHR or C (O) NRR’;

R⁵ is H, F, Cl or CF₃;

In certain embodiments, Ring A is a 6-membered heterocyclic ring, having the structural formula of (I^a) :

wherein

each of R¹ and R² is independently NH₂ or a C_1-6 alkyl, or R¹ and R², together with the carbon atom they are bound to, form a substituted or unsubstituted 5-membered carbocyclic or heterocyclic ring;

R³ is H, CH₃ or NH₂;

R⁴ is H, CH₂OH, C (O) OCH₃, C (O) NH₂, or C (O) NHCH₃;

R⁵ is H, Cl or CF₃;

each of R⁶, R⁷ and R⁸ is independently H, F, CN, Cl, OH, NH₂, CH₃, OCH₃, C (O) NH₂, 1, 2, 3-triazolyl, or P (O) RR’; and

each of R and R’ is independently a C_1-6 alkyl, cyclopropyl, cyclobutyl, or cyclopentyl.

In certain embodiments of formula (I^a) , Y¹ is CR⁷ and Y² is CR⁸, having the structural formula (II) :

In certain embodiments of formula (I^a) , Y¹ is N and Y² is CR⁸, having the structural formula (III) :

In certain embodiments of formula (I^a) , Y¹ is CR⁷ and Y² is N, having the structural formula (IV) :

In certain embodiments of formula (I^a) , X is S, having the structural formula (V) :

In certain embodiments of formula (V) , Y¹ is N, having the structural formula (VI) :

In certain embodiments of formula (V) , Y² is N, having the structural formula (VII) :

In certain embodiments of formula (V) , Y¹ is CR⁷ and Y² is CR⁸, having the structural formula (VIII) :

In certain embodiments of formula (I) , wherein X is a single bond, having the structural formula (IX) :

In certain embodiments of formula (IX) , Y¹ is N and Y² is CR⁸, having the structural formula (X) :

In certain embodiments of formula (IX) , Y¹ is CR⁷ and Y² is N, having the structural formula (XI) :

In certain embodiments of formula (IX) , Y¹ is CR⁷ and Y² is CR⁸, having the structural formula (XII) :

In certain embodiments of formula (I^a) , wherein R¹ and R² form a 5-membered carbocyclic or heterocyclic ring, optionally substituted, having the formula (XIII) :

wherein

Z is O or CH₂,

R⁹ is independently CH₃ or NH₂, and

i is 0, 1 or 2.

In certain embodiments of formula (V) , R¹ and R² form a 5-membered carbocyclic or heterocyclic ring, optionally substituted, having the formula (XIV) :

wherein

Z is O or CH₂,

R⁹ is independently CH₃ or NH₂, and

i is 0, 1 or 2.

In certain embodiments of formula (IX) , R¹ and R² form a 5-membered carbocyclic or heterocyclic ring, optionally substituted, having the formula (XV) :

wherein

Z is O or CH₂,

R⁹ is independently CH₃ or NH₂, and

i is 0, 1 or 2.

In certain embodiments of formula (XIII) , (XIV) or (XV) , Z is O.

In certain embodiments, i is 2, and two R⁹’s are NH₂ and CH₃.

In certain embodiments of formulae (I) - (XV) , R³ is NH₂.

In certain embodiments of formulae (I) - (XV) , R³ is H.

In certain embodiments of formulae (I) - (XV) , R³ is CH₃.

In certain embodiments of formulae (I) - (XV) , R⁴ is H.

In certain embodiments of formulae (I) - (XV) , R⁴ is CH₂OH.

In certain embodiments of formulae (I) - (XV) , R⁴ is C (O) OCH₃.

In certain embodiments of formulae (I) - (XV) , R⁴ is C (O) NH₂.

In certain embodiments of formulae (I) - (XV) , R⁴ is C (O) NHCH₃;

In certain embodiments of formulae (I) - (XV) , R⁵ is Cl.

In certain embodiments of formulae (I) - (XV) , R⁵ is H.

In certain embodiments of formulae (I) - (XV) , R⁵ is CF₃.

In certain embodiments of formulae (I) - (XV) , R⁶ is Cl.

In certain embodiments of formulae (I) - (XV) , one of R⁷ and R⁸ is F, CN, Cl, OH, NH₂, CH₃, OCH₃, C (O) NH₂ and 1, 2, 3-triazolyl.

In certain embodiments of formulae (I) - (XV) , one of R⁷ and R⁸ is P (O) RR’.

In certain embodiments of formulae (I) - (XV) , one of R and R’ is CH₃.

In certain embodiments of formulae (I) - (XV) , one of R and R’ is cyclopropyl.

In certain embodiments of formulae (I) - (XV) , one of R and R’ is cyclobutyl.

In certain embodiments of formulae (I) - (XV) , one of R and R’ is cyclopentyl.

Non-limiting examples of compounds of the invention include:

or a pharmaceutically acceptable form or an isotope derivative thereof.

In yet another aspect, the invention generally relates to a method for treating or reducing a disease or condition (e.g., mediated by the activity of SHP2) , comprising administering to a subject in need thereof a therapeutically effective amount of a pharmaceutical composition comprising a compound disclosed herein and a pharmaceutically acceptable excipient, carrier, or diluent.

In certain embodiments, the disease or condition is selected from breast cancer, lung cancer, Noonan syndrome, leopard syndrome, juvenile myelomonocytic leukemias, liver cancer, neuroblastoma, melanoma, squamous-cell carcinoma of the head and neck, acute myeloid leukemia, esophageal cancer, colon cancer, head cancer, gastric carcinoma, neuroblastoma, anaplastic large-cell lymphoma and glioblastoma.

In certain embodiments, the subject being treated is further administered one or more of chemotherapy, radiotherapy, targeted therapy, immunotherapy and hormonal therapy.

In certain embodiments, the method further comprises administering a chemotherapeutic agent to the subject.

In certain embodiments, the method further comprises administering a radiotherapy to the subject.

In certain embodiments, the method further comprises administering a targeted therapy to the subject.

In certain embodiments, the method further comprises administering an immunotherapy to the subject.

In certain embodiments, the method further comprises administering hormonal therapy to the subject.

As used herein, the term "chemotherapeutic agent" refers to a chemical compound useful in the treatment of cancer. Examples of chemotherapeutic agents include Erlotinib (Genentech/OSI Pharm. ) , Bortezomib (Millennium Pharm. ) , Fulvestrant (AstraZeneca) , Sutent (SU11248, Pfizer) , Letrozole (Novartis) , Imatinib mesylate (Novartis) , PTK787/ZK 222584 (Novartis) , Oxaliplatin (Sanofi) , 5-FU (5-fluorouracil) , Leucovorin, Rapamycin (Sirolimus, Wyeth) , Lapatinib (GSK572016, Glaxo Smith Kline) , Lonafarnib (SCH 66336) , Sorafenib (BAY43-9006, Bayer Labs) , and Gefitinib (AstraZeneca) , AG1478, AG1571 (SU 5271; Sugen) , alkylating agents such as thiotepa andcyclosphosphamide; alkyl sulfonates such as busulfan, improsulfan and piposulfan; aziridines such as benzodopa, carboquone, meturedopa, and uredopa; ethylenimines and methylamelamines including altretamine, triethylenemelamine, triethylenephosphoramide, triethylenethiophosphoramide and trimethylomelamine; acetogenins (especially bullatacin and bullatacinone) ; a camptothecin (including the synthetic analog topotecan) ; bryostatin; callystatin; CC-1065 (including its adozelesin, carzelesin and bizelesin synthetic analogs) ; cryptophycins (particularly cryptophycin 1 and cryptophycin 8) ; dolastatin; duocarmycin (including the synthetic analogs, KW-2189 and CB1-TM1) ; eleutherobin; pancratistatin; a sarcodictyin; spongistatin; nitrogen mustards such as chlorambucil, chlornaphazine, chlorophosphamide, estramustine, ifosfamide, mechlorethamine, mechlorethamine oxide hydrochloride, melphalan, novembichin, phenesterine, prednimustine, trofosfamide, uracil mustard; nitrosureas such as carmustine, chlorozotocin, fotemustine, lomustine, nimustine, and ranimnustine; antibiotics such as the enediyne antibiotics (e.g., calicheamicin, especially calicheamicin gammall and calicheamicin omegall (Angew Chem. Intl. Ed. Engl. (1994) 33: 183-186) ; dynemicin, including dynemicin A; bisphosphonates, such as clodronate; an esperamicin; as well as neocarzinostatin chromophore and related chromoprotein enediyne antibiotic chromophores) , aclacinomysins, actinomycin, authramycin, azaserine, bleomycins, cactinomycin, carabicin, caminomycin, carzinophilin, chromomycinis, dactinomycin, daunorubicin, detorubicin, 6-diazo-5-oxo-L-norleucine, (doxorubicin) , morpholino-doxorubicin, cyanomorpholino-doxorubicin, 2-pyrrolino-doxorubicin and deoxydoxorubicin) , epirubicin, esonibicin, idarubicin, marcellomycin, mitomycins such as mitomycin C, mycophenolic acid, nogalamycin, olivomycins, peplomycin, porfiromycin, puromycin, quelamycin, rodorubicin, streptonigrin, streptozocin, tubercidin, ubenimex, zinostatin, zorubicin; anti-metabolites such as methotrexate and 5-fluorouracil (5-FU) ; folic acid analogs such as denopterin, methotrexate, pteropterin, trimetrexate; purine analogs such as fludarabine, 6-mercaptopurine, thiamniprine, thioguanine; pyrimidine analogs such as ancitabine, azacitidine, 6-azauridine, carmofur, cytarabine, dideoxyuridine, doxifluridine, enocitabine, floxuridine; androgens such as calusterone, dromostanolone propionate, epitiostanol, mepitiostane, testolactone; anti-adrenals such as aminoglutethimide, mitotane, trilostane; folic acid replenisher such as frolinic acid; aceglatone; aldophosphamide glycoside; aminolevulinic acid; eniluracil; amsacrine; bestrabucil; bisantrene; edatraxate; defofamine; demecolcine; diaziquone; elformithine; elliptinium acetate; an epothilone; etoglucid; gallium nitrate; hydroxyurea; lentinan; lonidainine; maytansinoids such as maytansine and ansamitocins; mitoguazone; mitoxantrone; mopidanmol; nitraerine; pentostatin; phenamet; pirarubicin; losoxantrone; podophyllinic acid; 2-ethylhydrazide; procarbazine; polysaccharide complex (JHS Natural Products, Eugene, Oreg. ) ; razoxane; rhizoxin; sizofuran; spirogermanium; tenuazonic acid; triaziquone; 2, 2', 2"-trichlorotriethylamine; trichothecenes (especially T-2 toxin, verracurin A, roridin A and anguidine) ; urethan; vindesine; dacarbazine; mannomustine; mitobronitol; mitolactol; pipobroman; gacytosine; arabinoside ( "Ara-C" ) ; cyclophosphamide; thiotepa; taxoids, e.g., (paclitaxel; Bristol-Myers Squibb Oncology, Princeton, N. J. ) , (Cremophor-free) , albumin-engineered nanoparticle formulations of paclitaxel (American Pharmaceutical Partners, Schaumberg, 111. ) , and (doxetaxel; Rhone-Poulenc Rorer, Antony, France) ; chloranmbucil; (gemcitabine) ; 6-thioguanine; mercaptopurine; methotrexate; platinum analogs such as cisplatin and carboplatin; vinblastine; etoposide (VP-16) ; ifosfamide; mitoxantrone; vincristine; (vinorelbine) ; novantrone; teniposide; edatrexate; daunomycin; aminopterin; capecitabineibandronate; CPT-11; topoisomerase inhibitor RFS 2000; difluoromethylomithine (DMFO) ; retinoids such as retinoic acid; and pharmaceutically acceptable salts, acids and derivatives of any of the above.

Examples of the second (or further) agent or therapy may include, but are not limited to, immunotherapies (e.g. PD-1 inhibitors (pembrolizumab, nivolumab, cemiplimab) , PD-L1 inhibitors (atezolizumab, avelumab, durvalumab) , CTLA4 antagonist, cell signal transduction inhibitors (e.g., imatinib, gefitinib, bortezomib, erlotinib, sorafenib, sunitinib, dasatinib, vorinostat, lapatinib, temsirolimus, nilotinib, everolimus, pazopanib, trastuzumab, bevacizumab, cetuximab, ranibizumab, pegaptanib, panitumumab and the like) , mitosis inhibitors (e.g., paclitaxel, vincristine, vinblastine and the like) , alkylating agents (e.g., cisplatin, cyclophosphamide, chromabucil, carmustine and the like) , anti-metabolites (e.g., methotrexate, 5-FU and the like) , intercalating anticancer agents, (e.g., actinomycin, anthracycline, bleomycin, mitomycin-C and the like) , topoisomerase inhibitors (e.g., irinotecan, topotecan, teniposide and the like) , immunotherapic agents (e.g., interleukin, interferon and the like) and antihormonal agents (e.g., tamoxifen, raloxifene and the like) .

In yet another aspect, the invention generally relates to use of a compound disclosed herein for treating or reducing a disease or condition (e.g., mediated by the activity of SHP2) .

In yet another aspect, the invention generally relates to use of a compound disclosed herein and a pharmaceutically acceptable excipient, carrier, or diluent, in preparation of a medicament for treating or reducing a disease or condition (e.g., mediated by the activity of SHP2) .

In certain embodiments of method of treatment and use herein, the disease or condition is cancer, or a related disease or condition thereof.

As used herein, the terms "cancer" and "cancerous" refer to or describe the physiological condition in mammals that is typically characterized by abnormal or unregulated cell growth. In certain embodiments, the cancer may be selected from melanoma, juvenile myelomoncytic leukemias, neuroblastoma, Philadelphia chromosome positive chronic myeloid, Philadelphia chromosome positive acute lymphoblastic leukemias, acute myeloid leukemias, myeloproliferative neoplasms (such as Polycythemia Vera, Essential Thrombocythemia and Primary Myelofibrosis) , breast cancer, lung cancer, liver cancer, colorectal cancer, esophageal cancer, gastric cancer, squamous-cell carcinoma of the head and neck, glioblastoma, anaplastic large-cell lymphoma, thyroid carcinoma, and spitzoid neoplasms. In certain embodiments, the cancer is melanoma. In certain embodiments, the cancer is juvenile myelomoncytic leukemias. In certain embodiments, the cancer is neuroblastoma. In certain embodiments, the cancer is Philadelphia chromosome positive chronic myeloid. In certain embodiments, the cancer is Philadelphia chromosome positive acute lymphoblastic leukemias. In certain embodiments, the cancer is acute myeloid leukemias. In certain embodiments, the cancer is myeloproliferative neoplasms, such as Polycythemia Vera, Essential Thrombocythemia and Primary Myelofibrosis. In certain embodiments, the cancer is selected from the group consisting of Polycythemia Vera, Essential Thrombocythemia and Primary Myelofibrosis. In certain embodiments, the cancer is Polycythemia Vera. In certain embodiments, the cancer is Essential Thrombocythemia. In certain embodiments, the cancer is Primary Myelofibrosis. In certain embodiments, the cancer is breast cancer. In certain embodiments, the cancer is lung cancer. In certain embodiments, the cancer is liver cancer. In certain embodiments, the cancer is colorectal cancer. In certain embodiments, the cancer is esophageal cancer. In certain embodiments, the cancer is gastric cancer. In certain embodiments, the cancer is squamous-cell carcinoma of the head and neck. In certain embodiments, the cancer is glioblastoma. In certain embodiments, the cancer is anaplastic large-cell lymphoma. In certain embodiments, the cancer is thyroid carcinoma. In certain embodiments, the cancer is spitzoid neoplasms.

In certain embodiments, the cancer is selected from the group consisting of non-small cell lung cancer (NSCLC) , a colon cancer, an esophageal cancer, a rectal cancer, Juvenile myelomonocytic leukemia (JMML) , breast cancer, melanoma, and a pancreatic cancer.

Compositions of the present invention are administered orally, parenterally, by inhalation spray, topically, rectally, nasally, buccally, vaginally or via an implanted reservoir. The term "parenteral" as used herein includes subcutaneous, intravenous, intramuscular, intra-articular, intra-synovial, intrasternal, intrathecal, intrahepatic, intralesional and intracranial injection or infusion techniques. Preferably, the compositions are administered orally, intraperitoneally or intravenously. Sterile injectable forms of the compositions of this invention include aqueous or oleaginous suspension. These suspensions are formulated according to techniques known in the art using suitable dispersing or wetting agents and suspending agents. The sterile injectable preparation is also be a sterile injectable solution or suspension in a non-toxic parenterally acceptable diluent or solvent, for example as a solution in 1, 3-butanediol. Among the acceptable vehicles and solvents that are employed are water, Ringer’s solution and isotonic sodium chloride solution. In addition, sterile, fixed oils are conventionally employed as a solvent or suspending medium.

For this purpose, any bland fixed oil employed includes synthetic mono-or di-glycerides. Fatty acids, such as oleic acid and its glyceride derivatives are useful in the preparation of injectables, as are natural pharmaceutically-acceptable oils, such as olive oil or castor oil, especially in their polyoxyethylated versions. These oil solutions or suspensions also contain a long-chain alcohol diluent or dispersant, such as carboxymethyl cellulose or similar dispersing agents that are commonly used in the formulation of pharmaceutically acceptable dosage forms including emulsions and suspensions. Other commonly used surfactants, such as Tweens, Spans and other emulsifying agents or bioavailability enhancers which are commonly used in the manufacture of pharmaceutically acceptable solid, liquid, or other dosage forms are also be used for the purposes of formulation.

Pharmaceutically acceptable compositions of this invention are orally administered in any orally acceptable dosage form. Exemplary oral dosage forms are capsules, tablets, aqueous suspensions or solutions. In the case of tablets for oral use, carriers commonly used include lactose and corn starch. Lubricating agents, such as magnesium stearate, are also typically added. For oral administration in a capsule form, useful diluents include lactose and dried cornstarch. When aqueous suspensions are required for oral use, the active ingredient is combined with emulsifying and suspending agents. If desired, certain sweetening, flavoring or coloring agents are optionally also added.

Alternatively, pharmaceutically acceptable compositions of this invention are administered in the form of suppositories for rectal administration. These can be prepared by mixing the agent with a suitable non-irritating excipient that is solid at room temperature but liquid at rectal temperature and therefore will melt in the rectum to release the drug. Such materials include cocoa butter, beeswax and polyethylene glycols.

Pharmaceutically acceptable compositions of this invention are also administered topically, especially when the target of treatment includes areas or organs readily accessible by topical application, including diseases of the eye, the skin, or the lower intestinal tract. Suitable topical formulations are readily prepared for each of these areas or organs.

Topical application for the lower intestinal tract can be effected in a rectal suppository formulation (see above) or in a suitable enema formulation. Topically-transdermal patches are also used.

For topical applications, provided pharmaceutically acceptable compositions are formulated in a suitable ointment containing the active component suspended or dissolved in one or more carriers. Exemplary carriers for topical administration include mineral oil, liquid petrolatum, white petrolatum, propylene glycol, polyoxyethylene, polyoxypropylene compound, emulsifying wax and water. Alternatively, provided pharmaceutically acceptable compositions can be formulated in a suitable lotion or cream containing the active components suspended or dissolved in one or more pharmaceutically acceptable carriers. Suitable carriers include, but are not limited to, mineral oil, sorbitan monostearate, polysorbate 60, cetyl esters wax, cetearyl alcohol, 2-octyldodecanol, benzyl alcohol and water.

Pharmaceutically acceptable compositions of this invention are optionally administered by nasal aerosol or inhalation. Such compositions are prepared according to techniques well-known in the art of pharmaceutical formulation and are prepared as solutions in saline, employing benzyl alcohol or other suitable preservatives, absorption promoters to enhance bioavailability, fluorocarbons, and/or other conventional solubilizing or dispersing agents.

Most preferably, pharmaceutically acceptable compositions of this invention are formulated for oral administration. Such formulations may be administered with or without food. In some embodiments, pharmaceutically acceptable compositions of this invention are administered without food. In other embodiments, pharmaceutically acceptable compositions of this invention are administered with food.

The amount of compounds of the present invention that is optionally combined with the carrier materials to produce a composition in a single dosage form will vary depending upon the host treated, the particular mode of administration. Preferably, provided compositions should be formulated so that a dosage of between 0.01 -100 mg/kg body weight/day of the compound can be administered to a patient receiving these compositions.

It should also be understood that a specific dosage and treatment regimen for any particular patient will depend upon a variety of factors, including the activity of the specific compound employed, the age, body weight, general health, sex, diet, time of administration, rate of excretion, drug combination, and the judgment of the treating physician and the severity of the particular disease being treated. The amount of a compound of the present invention in the composition will also depend upon the particular compound in the composition.

Examples

As depicted in the Examples below, in certain exemplary embodiments, compounds are prepared according to the following general procedures. It will be appreciated that, although the general methods depict the synthesis of certain compounds of the present invention, the following general methods, and other methods known to one of ordinary skill in the art, can be applied to all compounds and subclasses and species of each of these compounds, as described herein.

Compound numbers utilized in the Examples below correspond to compound numbers set forth supra.

¹H was recorded at 400 MHz on a Varian Mercury 400 spectrometer. ¹³C NMR was recorded at 100 MHz. Proton chemical shifts were internally referenced to the residual proton resonance in CDCl3 (7.26 ppm) . Carbon chemical shifts were internally referenced to the deuterated solvent signals in CDCl3 (77.20 ppm) .

LC-MS spectra were recorded on a Shimadzu LC-MS2020 using Agilent C18 column (Eclipse XDB-C18, 5um, 2.1 x 50mm) with flow rate of 1 mL/min. Mobile phase A: 0.1%of formic acid in water; mobile phase B: 0.1%of formic acid in acetonitrile. A general gradient method was used.

Table 1

Analytical HPLC was performed on Agilent 1200 HPLC with a Zorbax Eclipse XDB C18 column (2.1 x 150 mm) with flow rate of 1 mL/min. Mobile phase A: 0.1%of TFA in water; mobile phase B: 0.1%of TFA in acetonitrile. A general method with following gradient was used.

Table 2

Preparative HPLC was performed on Varian ProStar using Hamilton C18 PRP-1 column (15 x 250 mm) with flow rate of 20 mL/min. Mobile phase A: 0.1%of TFA in water; mobile phase B: 0.1%of TFA in acetonitrile. A typical gradient method was used.

Table 3

Example 1.

Synthesis of (4- ( (3-amino-5- ( (3S, 4S) -4-amino-3-methyl-2-oxa-8-azaspiro [4.5] decan-8-yl) pyrazin-2-yl) thio) -2, 3-dichlorophenyl) dimethylphosphine oxide (1)

Synthetic scheme 1

Step 1: A mixture of compound 1-1 (1.0 g, 4.80 mmol) , Xantphos (280 mg, 0.484 mmol) , Pd (OAc) ₂ (108 mg, 0.481 mmol) in a Schlenk vial was purged with nitrogen for 3 times. To this was added a solution of compound 1-2 (670 mg, 5.57 mmol) and DIPEA (1.85 g, 14.34 mmol) in dioxane. The reaction mixture was purged again with nitrogen for 3 times and stirred at 105 ℃ for 16 h. After completion of the reaction, the mixture was cooled to room temperature and filtered. The filtrate was concentrated and purified by silica gel column (eluted with 30%EtOAc in Petroleum ether) to afford the title compound 1-3 (950 mg, yield 80%) as a light-yellow solid.

Step 2: Under nitrogen atmosphere, to a solution of compound 1-3 (500 mg, 2.02 mmol) in anhydrous THF (8 mL) was added dropwise NaOEt (20 w%solution in EtOH, 1.1 g, 3.23 mmol) at -30 ℃. The mixture was stirred at -30 ℃ for 2 h. After completion of the reaction, the mixture was warmed to room temperature and concentrated. The residue was acidified by 1 N hydrochloric acid and purified by reverse phase flash (C18 column, eluted with acetonitrile in H₂O, HCl condition) . The desired components were lyophilized to give the title compound 1-4 (hydrochloride, 320 mg, yield 80%) as a yellow solid.

Step 3: To a solution of compound 1-5 (10.0 g, 61.7 mmol) in DMF (60 mL) was added NIS (14.9 g, 66.2 mmol) portion-wise over 30 min at 0 ℃. The mixture was stirred at room temperature for 8 h. After completion of the reaction, the mixture was poured in H₂O (600 mL) . The resulted mixture was extracted with EtOAc (300 mL x 2) . The combined organic layers were washed with water and brine, dried over anhydrous Na₂SO₄, filtered and concentrated. The crude product was purified by silica gel column (eluted with 10%EtOAc in Petroleum ether) to afford the title compound 1-6 (11.2 g, yield 63%) as brown solid.

Step 4: Under nitrogen atmosphere, a mixture of compound 1-6 (10.0 g, 34.7 mmol) , compound 1-7 (8.13 g, 103 mmol) , Xantphos (2.0 g, 3.45 mmol) , Pd (OAc) ₂ (390 mg, 1.74 mmol) and K₃PO₄ (18.4 g, 86.7 mmol) in anhydrous DMF (50 mL) was stirred at 120 ℃ for 16 h. The mixture was cooled to room temperature, filtered and concentrated. The residue was purified by reverse phase flash (C18 column, eluted with acetonitrile in H₂O, HCl condition) . The desired components were lyophilized to give the title compound 1-8 (hydrochloride, 1.35 g, yield 14%) as a yellow solid.

Step 5: To a solution of compound 1-8 (hydrochloride, 930 mg, 3.36 mmol) in MeCN (9 mL) was added 4 N aqueous HCl (3.5 mL, 14 mmol) . The mixture was stirred at room temperature for 10 min and cooled to 0 ℃. To this was added a cold solution of NaNO₂ (280 mg, 4.05 mmol) in H₂O (2 mL) . The mixture was stirred at 0 ℃ for 30 min. CuI (64 mg, 0.336 mmol) and KI (1.38 g, 8.31 mmol) were added thereto. The mixture was stirred at room temperature for 3 h. After completion of the reaction, the mixture was quenched by adding H₂O (50 mL) and extracted with EtOAc (50 mL x 2) . The combined organic layers were washed with saturated NaHCO₃ and saturated Na₂S₂O₃, water and brine. The organic layer was dried over anhydrous Na₂SO₄, filtered and concentrated. The crude product was purified by silica gel column (eluted with 5%EtOAc in Petroleum ether) to afford the title compound 1-9 (720 mg, yield 61%) as brown oil.

Step 6: A mixture of compound 1-9 (500 mg, 1.43 mmol) , compound 1-4 (hydrochloride, 350 mg, 1.76 mmol) , Xantphos (165 mg, 0.285 mmol) , Pd₂ (Dba) ₃ (130 mg, 0.142 mmol) and DIPEA (550 mg, 4.25 mmol) in dioxane (5 mL) was stirred at 115 ℃ for 16 h. After completion of the reaction, the mixture was cooled to room temperature, filtered and concentrated. The residue was purified by silica gel column (eluted with 5%methanol in dichloromethane) to afford the crude product, which was further purified by reverse phase flash (C18 column, eluted with acetonitrile in H₂O, HCl condition) . The desired components were lyophilized to give the title compound 1-10 (hydrochloride, 255 mg, yield 42%) as an off-white solid.

Step 7: A mixture of compound 1-10 (hydrochloride, 100 mg, 0.239 mmol) , compound 1-11 (70 mg, 0.288 mmol) and K₂CO₃ (165 mg, 1.19 mmol) in DMAc (5 mL) and H₂O (0.5 mL) was stirred at 120 ℃ for 18 h. After completion of the reaction, the mixture was cooled and filtered. The filtrate was purified by reverse phase flash (C18 column, eluted with acetonitrile in H₂O, HCl condition) . The desired components were lyophilized to give the title compound 1 (65 mg, yield 49%) as an off-white solid. ¹H NMR (400 MHz, DMSO-d₆ (D₂O)) δ7.72 –7.61 (m, 2H) , 6.69 (d, J = 9.0 Hz, 1H) , 4.24 –4.17 (m, 2H) , 4.13 (d, J = 16.5 Hz, 1H) , 3.89 (d, J = 9.1 Hz, 1H) , 3.69 (s, 1H) , 3.36 (d, J = 5.0 Hz, 1H) , 3.10 –2.98 (m, 2H) , 1.80 (s, 3H) , 1.76 (s, 3H) , 1.75 –1.64 (m, 3H) , 1.56 (d, J = 12.6 Hz, 1H) , 1.21 (d, J = 6.5 Hz, 3H) . LCMS: m/z calculated for C₂₁H₂₈Cl₂N₅O₂PS: 516.42; found: 516.71 [M+H] ⁺.

Example 2.

Synthesis of (4- ( (3-amino-5- ( (3S, 4S) -4-amino-3-methyl-2-oxa-8-azaspiro [4.5] decan-8-yl) pyrazin-2-yl) thio) -2-chloro-3- (trifluoromethyl) phenyl) dimethylphosphine oxide (2)

Synthetic scheme 2

Step 1: To a solution of compound 2-1 (10.0 g, 51.1 mmol) in DMF (50 mL) was added NBS (10.0 g, 56.2 mmol) portion-wise over 30 min at 0 ℃. The mixture was stirred at room temperature for 3 h. After completion of the reaction, the mixture was poured in H₂O (500 mL) . The resulted mixture was extracted with EtOAc (300 mL x 2) . The combined organic layers were washed with water and brine, dried over anhydrous Na₂SO₄, filtered and concentrated. The crude product was purified by silica gel column (eluted with 10%EtOAc in Petroleum ether) to afford the title compound 2-2 (11.9 g, yield 85%) as brown liquid.

Step 2: To a solution of compound 2-2 (3 g, 10.9 mmol) in MeCN (10 mL) was added 4 N aqueous HCl (11 mL, 44 mmol) . The mixture was stirred at room temperature for 10 min and cooled to 0 ℃. To this was added a cold solution of NaNO₂ (900 mg, 13.0 mmol) in H₂O (5 mL) . The mixture was stirred at 0 ℃ for 30 min. CuI (210 mg, 1.10 mmol) and KI (4.5 g, 27.1 mmol) were added thereto. The mixture was stirred at room temperature for 3 h. After completion of the reaction, the mixture was quenched by adding H₂O (150 mL) and extracted with EtOAc (150 mL x 2) . The combined organic layers were washed with saturated NaHCO₃ and saturated Na₂S₂O₃, water and brine. The organic layer was dried over anhydrous Na₂SO₄, filtered and concentrated. The crude product was purified by silica gel column (eluted with Petroleum ether) to afford the title compound 2-3 (3 g, yield 70%) as brown oil.

Step 3: Under nitrogen atmosphere, a mixture of compound 2-3 (4.68 g, 12.1 mmol) , compound 1-7 (1.05 g, 13.4 mmol) , Xantphos (1.4 g, 2.42 mmol) , Pd (OAc) ₂ (270 mg, 1.20 mmol) and K₃PO₄ (6.5 g, 30.6 mmol) in anhydrous DMF (30 mL) was stirred at 120 ℃ for 16 h. The mixture was cooled to room temperature, filtered and concentrated. The residue was purified by reverse phase flash (C18 column, eluted with acetonitrile in H₂O, HCl condition) . The desired components were lyophilized to give the title compound 2-4 (820 mg, yield 20%) as a yellow solid.

Step 4: A mixture of compound 2-4 (300 mg, 0.894 mmol) , compound 1-4 (hydrochloride, 260 mg, 1.32 mmol) , Xantphos (103 mg, 0.178 mmol) , Pd₂ (Dba) ₃ (82 mg, 0.089 mmol) and DIPEA (350 mg, 2.71 mmol) in dioxane (3 mL) was stirred at 115 ℃ for 16 h. After completion of the reaction, the mixture was cooled to room temperature, filtered and concentrated. The residue was purified by silica gel column (eluted with 5%methanol in dichloromethane) to afford the crude product, which was further purified by reverse phase flash (C18 column, eluted with acetonitrile in H₂O, HCl condition) . The desired components were lyophilized to give the title compound 2-5 (hydrochloride, 165 mg, yield 40%) as a pale yellow solid.

Step 5: A mixture of compound 2-5 (hydrochloride, 100 mg, 0.221 mmol) , compound 1-11 (65 mg, 0.267 mmol) and K₂CO₃ (150 mg, 1.08 mmol) in DMAc (3 mL) and H₂O (0.3 mL) was stirred at 120 ℃ for 18 h. After completion of the reaction, the mixture was cooled and filtered. The filtrate was purified by reverse phase flash (C18 column, eluted with acetonitrile in H₂O, HCl condition) . The desired components were lyophilized to give the title compound 2 (19 mg, yield 49%) as a pale-yellow solid. ¹H NMR (500 MHz, DMSO-d₆) δ 8.25 (d, J = 5.6 Hz, 2H) , 7.91 (dd, J = 11.1, 8.6 Hz, 1H) , 7.71 (s, 1H) , 6.90 (d, J = 8.5 Hz, 1H) , 4.27 –4.19 (m, 2H) , 4.15 (d, J = 13.8 Hz, 1H) , 3.93 (s, 1H) , 3.66 (d, J = 9.1 Hz, 1H) , 3.40 –3.35 (m, 1H) , 3.07 (q, J = 11.1 Hz, 2H) , 1.84 –1.73 (m, 8H) , 1.67 (d, J = 13.5 Hz, 1H) , 1.58 (d, J = 12.9 Hz, 1H) , 1.24 (d, J = 6.7 Hz, 3H) . LCMS: m/z calculated for C₂₂H₂₈ClF₃N₅O₂PS: 549.98; found: 550.60 [M+H] ⁺.

Example 3.

Synthesis of (4- ( (3-amino-5- ( (3S, 4S) -4-amino-3-methyl-2-oxa-8-azaspiro [4.5] decan-8-yl) pyrazin-2-yl) thio) -3-chlorophenyl) dimethylphosphine oxide (3)

Synthetic scheme 3

Step 1: Under nitrogen atmosphere, a mixture of compound 3-1 (5.0 g, 19.7 mmol) , compound 1-7 (4.61 g, 59.1 mmol) , Xantphos (2.28 g, 3.94 mmol) , Pd (OAc) ₂ (442 mg, 1.97 mmol) and K₃PO₄ (10.5 g, 49.5 mmol) in anhydrous DMF (50 mL) was stirred at 120 ℃ for 16 h. The mixture was cooled to room temperature, filtered and concentrated. The residue was purified by silica gel column (eluted with 5%methanol in dichloromethane) to afford the title compound 3-2 (3.69 g, yield 67%) as a yellow solid.

Step 2: To a solution of compound 3-2 (2 g, 9.82 mmol) in MeCN (10 mL) was added 4 N aqueous HCl (10 mL, 40 mmol) . The mixture was stirred at room temperature for 10 min and cooled to 0 ℃. To this was added a cold solution of NaNO₂ (735 mg, 10.6 mmol) in H₂O (5 mL) . The mixture was stirred at 0 ℃ for 30 min. CuI (190 mg, 0.997 mmol) and KI (4.07 g, 24.5 mmol) were added thereto. The mixture was stirred at room temperature for 3 h. After completion of the reaction, the mixture was quenched by adding H₂O (100 mL) and extracted with EtOAc (100 mL x 2) . The combined organic layers were washed with saturated NaHCO₃ and saturated Na₂S₂O₃, water and brine. The organic layer was dried over anhydrous Na₂SO₄, filtered and concentrated. The crude product was purified by silica gel column (eluted with Petroleum ether) to afford the title compound 3-3 (400 mg, yield 13%) as brown oil.

Step 3: A mixture of compound 3-3 (220 mg, 0.70 mmol) , compound 1-4 (hydrochloride, 210 mg, 1.06 mmol) , Xantphos (85 mg, 0.147 mmol) , Pd₂ (Dba) ₃ (64 mg, 0.07 mmol) and DIPEA (270 mg, 2.09 mmol) in dioxane (3 mL) was stirred at 115 ℃ for 16 h. After completion of the reaction, the mixture was cooled to room temperature, filtered and concentrated. The residue was purified by silica gel column (eluted with 5%methanol in dichloromethane) to afford the crude product, which was further purified by reverse phase flash (C18 column, eluted with acetonitrile in H₂O, HCl condition) . The desired components were lyophilized to give the title compound 3-4 (hydrochloride, 98 mg, yield 36%) as a pale-yellow solid.

A mixture of compound 3-4 (hydrochloride, 60 mg, 0.156 mmol) , compound 1-11 (45 mg, 0.185 mmol) and K₂CO₃ (110 mg, 0.796 mmol) in DMAc (3 mL) and H₂O (0.3 mL) was stirred at 120 ℃ for 18 h. After completion of the reaction, the mixture was cooled and filtered. The filtrate was purified by reverse phase flash (C18 column, eluted with acetonitrile in H₂O, HCl condition) . The desired components were lyophilized to give the title compound 3 (35 mg, yield 44%) as a pale-yellow solid. ¹H NMR (600 MHz, DMSO-d₆) δ 8.47 (d, J = 47.8 Hz, 1H) , 8.35 (d, J = 33.5 Hz, 2H) , 7.85 (s, 1H) , 7.66 (d, J = 3.6 Hz, 1H) , 7.59 (q, J = 7.4, 6.6 Hz, 1H) , 4.24 –4.18 (m, 2H) , 4.12 (dd, J = 14.1, 4.3 Hz, 1H) , 3.94 –3.90 (m, 1H) , 3.65 (dd, J = 9.2, 3.5 Hz, 1H) , 3.36 (t, J = 4.5 Hz, 1H) , 3.05 (q, J = 12.0, 11.5 Hz, 2H) , 1.77 (td, J = 12.6, 11.9, 6.0 Hz, 2H) , 1.67 (s, 1H) , 1.65 –1.63 (m, 3H) , 1.62 (d, J = 2.5 Hz, 3H) , 1.58 (d, J = 12.8 Hz, 1H) , 1.24 (d, J = 6.5 Hz, 3H) . LCMS: m/z calculated for C₂₁H₂₉ClN₅O₂PS: 481.98; found: 482.95 [M+H] ⁺.

Example 4.

Synthesis of (3- ( (3-amino-5- ( (3S, 4S) -4-amino-3-methyl-2-oxa-8-azaspiro [4.5] decan-8-yl) pyrazin-2-yl) thio) -5-chlorophenyl) dimethylphosphine oxide (4)

Synthetic scheme 4

Step 1: Under nitrogen atmosphere, a mixture of compound 4-1 (2.1 g, 6.61 mmol) , compound 1-7 (570 mg, 7.30 mmol) , Xantphos (760 mg, 1.31 mmol) , Pd (OAc) ₂ (150 mg, 0.668 mmol) and K₃PO₄ (3.50 g, 16.5 mmol) in anhydrous DMF (20 mL) was stirred at 120 ℃ for 16 h. The mixture was cooled to room temperature and filtered. The filtrate was diluted with EtOAc (100 mL) and washed with water (50 mL x2) . The organic layer was washed with brine, dried over anhydrous Na₂SO₄, filtered and concentrated. The residue was purified by silica gel column (eluted with 5%methanol in dichloromethane) to afford the title compound 4-2 (750 mg, yield 42%) as a yellow solid.

Step 2: A mixture of compound 4-2 (220 mg, 0.822 mmol) , compound 1-4 (hydrochloride, 195 mg, 0.929 mmol) , Xantphos (95 mg, 0.164 mmol) , Pd₂ (Dba) ₃ (75 mg, 0.082 mmol) and DIPEA (320 mg, 2.48 mmol) in dioxane (4 mL) was stirred at 115 ℃ for 16 h. After completion of the reaction, the mixture was cooled to room temperature, filtered and concentrated. The residue was purified by silica gel column (eluted with 5%methanol in dichloromethane) to afford the crude product, which was further purified by reverse phase flash (C18 column, eluted with acetonitrile in H₂O, HCl condition) . The desired components were lyophilized to give the title compound 4-3 (hydrochloride, 65 mg, yield 20%) as a pale-yellow solid.

Step 3: A mixture of compound 4-3 (hydrochloride, 60 mg, 0.156 mmol) , compound 1-11 (45 mg, 0.185 mmol) and K₂CO₃ (110 mg, 0.796 mmol) in DMAc (3 mL) and H₂O (0.3 mL) was stirred at 120 ℃ for 18 h. After completion of the reaction, the mixture was cooled and filtered. The filtrate was purified by reverse phase flash (C18 column, eluted with acetonitrile in H₂O, HCl condition) . The desired components were lyophilized to give the title compound 4 (23 mg, yield 44%) as a pale-yellow solid. ¹H NMR (600 MHz, DMSO-d₆) δ 8.32 (d, J = 32.8 Hz, 2H) , 7.79 (dd, J = 11.2, 1.5 Hz, 1H) , 7.70 (d, J = 3.8 Hz, 1H) , 7.56 (ddd, J = 10.2, 8.0, 1.7 Hz, 1H) , 6.75 (tt, J = 6.8, 2.3 Hz, 1H) , 4.23 (d, J = 11.4 Hz, 1H) , 4.21 –4.19 (m, 1H) , 4.15 –4.12 (m, 1H) , 3.92 (dd, J = 9.1, 3.4 Hz, 1H) , 3.66 (dd, J = 9.3, 4.0 Hz, 1H) , 3.41 –3.34 (m, 1H) , 3.12 –3.00 (m, 2H) , 1.82 –1.74 (m, 2H) , 1.67 (d, J = 14.4 Hz, 1H) , 1.64 (d, J = 1.2 Hz, 3H) , 1.62 (d, J = 1.2 Hz, 3H) , 1.59 (d, J = 13.5 Hz, 1H) , 1.24 (d, J = 6.7 Hz, 3H) . LCMS: m/z calculated for C₂₁H₂₉ClN₅O₂PS: 481.98; found: 482.89 [M+H] ⁺.

Example 5.

Synthesis of (5- ( (3-amino-5- ( (3S, 4S) -4-amino-3-methyl-2-oxa-8-azaspiro [4.5] decan-8-yl) pyrazin-2-yl) thio) pyridin-2-yl) dimethylphosphine oxide (5)

Synthetic scheme 5

Step 1: Under nitrogen atmosphere, a mixture of compound 5-1 (5.0 g, 28.9 mmol) , compound 1-7 (6.76 g, 86.7 mmol) , Xantphos (3.34 g, 5.77 mmol) , Pd (OAc) ₂ (650 mg, 2.89 mmol) and K₃PO₄ (15.3 g, 72.1 mmol) in anhydrous DMF (50 mL) was stirred at 120 ℃ for 16 h. The mixture was cooled to room temperature, filtered and concentrated. The residue was purified by silica gel column (eluted with 10%methanol in dichloromethane) to afford the crude product, which was purified again by reverse phase flash (C18 column, eluted with acetonitrile in H₂O, HCl condition) . The desired components were lyophilized to give the title compound 5-2 (hydrochloride, 2.45 g, yield 41%) as a yellow solid.

Step 2: To a solution of compound 5-2 (hydrochloride, 2.7 g, 13.0 mmol) in MeCN (30 mL) was added 4 N aqueous HCl (15 mL, 60 mmol) . The mixture was stirred at room temperature for 10 min and cooled to 0 ℃. To this was added dropwise a cold solution of NaNO₂ (950 mg, 13.8 mmol) in H₂O (10 mL) . The mixture was stirred at 0 ℃ for 30 min. CuI (250 mg, 1.31 mmol) and KI (5.44 g, 32.8 mmol) were added thereto. The mixture was stirred at room temperature for 3 h. After completion of the reaction, the mixture was quenched by adding H₂O (150 mL) and extracted with EtOAc (150 mL x 2) . The combined organic layers were washed with saturated NaHCO₃ and saturated Na₂S₂O₃, water and brine. The organic layer was dried over anhydrous Na₂SO₄, filtered and concentrated. The crude product was purified by silica gel column (eluted with 10%methanol in dichloromethane) to afford the title compound 5-3 (1.33 g, yield 36%) as a yellow oil.

Step 3: A mixture of compound 5-3 (300 mg, 1.07 mmol) , compound 1-4 (hydrochloride, 320 mg, 1.61 mmol) , Xantphos (123 mg, 0.212 mmol) , Pd₂ (Dba) ₃ (98 mg, 0.107 mmol) and DIPEA (415 mg, 3.21 mmol) in dioxane (5 mL) was stirred at 115 ℃ for 16 h. After completion of the reaction, the mixture was cooled to room temperature, filtered and concentrated. The residue was purified by silica gel column (eluted with 20%methanol in dichloromethane) to afford the crude product, which was further purified by reverse phase flash (C18 column, eluted with acetonitrile in H₂O, HCl condition) . The desired components were lyophilized to give the title compound 5-4 (hydrochloride, 60 mg, yield 16%) as a yellow solid.

Step 4: A mixture of compound 5-4 (hydrochloride, 60 mg, 0.171 mmol) , compound 1-11 (62 mg, 0.255 mmol) and K₂CO₃ (120 mg, 0.868 mmol) in DMAc (3 mL) and H₂O (0.3 mL) was stirred at 120 ℃ for 18 h. After completion of the reaction, the mixture was cooled and filtered. The filtrate was purified by reverse phase flash (C18 column, eluted with acetonitrile in H₂O, HCl condition) . The desired components were lyophilized to give the title compound 5 (38 mg, yield 45%) as a yellow solid. ¹H NMR (600 MHz, DMSO-d₆) δ 8.30 (d, J = 30.8 Hz, 1H) , 8.23 (s, 1H) , 7.67 (s, 1H) , 7.61 (d, J = 11.3 Hz, 1H) , 7.45 (d, J = 11.2 Hz, 1H) , 7.21 (s, 1H) , 4.22 (dq, J = 12.8, 6.5, 5.4 Hz, 2H) , 4.17 –4.12 (m, 1H) , 3.94 (dd, J = 9.2, 2.2 Hz, 1H) , 3.67 (d, J = 9.1 Hz, 1H) , 3.37 (t, J = 4.6 Hz, 1H) , 3.10 –3.01 (m, 2H) , 1.76 (dd, J = 16.6, 7.2 Hz, 2H) , 1.68 (d, J = 3.2 Hz, 4H) , 1.66 (d, J = 3.1 Hz, 3H) , 1.58 (d, J = 13.1 Hz, 1H) , 1.26 (d, J = 6.6 Hz, 3H) . LCMS: m/z calculated for C₂₀H₂₉N₆O₂PS: 448.53; found: 449.82 [M+H] ⁺.

Example 6.

Synthesis of (5- ( (3-amino-5- ( (3S, 4S) -4-amino-3-methyl-2-oxa-8-azaspiro [4.5] decan-8-yl) pyrazin-2-yl) thio) pyridin-3-yl) dimethylphosphine oxide (6)

Synthetic scheme 6

Step 1: Under nitrogen atmosphere, a mixture of compound 6-1 (5.0 g, 28.9 mmol) , compound 1-7 (6.76 g, 86.7 mmol) , Xantphos (3.34 g, 5.77 mmol) , Pd (OAc) ₂ (650 mg, 2.89 mmol) and K₃PO₄ (15.3 g, 72.1 mmol) in anhydrous DMF (50 mL) was stirred at 120 ℃ for 16 h. The mixture was cooled to room temperature, filtered and concentrated. The residue was purified by silica gel column (eluted with 10%methanol in dichloromethane) to afford the crude product, which was purified again by reverse phase flash (C18 column, eluted with acetonitrile in H₂O, HCl condition) . The desired components were lyophilized to give the title compound 6-2 (hydrochloride, 2.2 g, yield 36%) as a yellow solid.

Step 2: To a solution of compound 6-2 (hydrochloride, 1.7 g, 8.22 mmol) in MeCN (20 mL) was added 4 N aqueous HCl (10 mL, 40 mmol) . The mixture was stirred at room temperature for 10 min and cooled to 0 ℃. To this was added dropwise a cold solution of NaNO₂ (600 mg, 8.70 mmol) in H₂O (6 mL) . The mixture was stirred at 0 ℃ for 30 min. CuI (150 mg, 0.787 mmol) and KI (3.41 g, 20.5 mmol) were added thereto. The mixture was stirred at room temperature for 3 h. After completion of the reaction, the mixture was quenched by adding H₂O (150 mL) and extracted with EtOAc (150 mL x 2) . The combined organic layers were washed with saturated NaHCO₃ and saturated Na₂S₂O₃, water and brine. The organic layer was dried over anhydrous Na₂SO₄, filtered and concentrated. The crude product was purified by silica gel column (eluted with 10%methanol in dichloromethane) to afford the title compound 6-3 (200 mg, yield 8%) as a yellow oil.

Step 3: A mixture of compound 6-3 (130 mg, 0.462 mmol) , compound 1-4 (hydrochloride, 130 mg, 0.656 mmol) , Xantphos (55 mg, 0.095 mmol) , Pd₂ (Dba) ₃ (45 mg, 0.049 mmol) and DIPEA (180 mg, 1.39 mmol) in dioxane (2mL) was stirred at 115 ℃ for 16 h. After completion of the reaction, the mixture was cooled to room temperature, filtered and concentrated. The residue was purified by pre-TLC (silica gel, 20%methanol in dichloromethane) to afford the crude product, which was further purified by reverse phase flash (C18 column, eluted with acetonitrile in H₂O, HCl condition) . The desired components were lyophilized to give the title compound 6-4 (hydrochloride, 40 mg, yield 24%) as a yellow solid.

Step 4: A mixture of compound 6-4 (hydrochloride, 40 mg, 0.114 mmol) , compound 1-11 (40 mg, 0.165 mmol) and K₂CO₃ (80 mg, 0.578 mmol) in DMAc (2 mL) and H₂O (0.2 mL) was stirred at 120 ℃ for 18 h. After completion of the reaction, the mixture was cooled and filtered. The filtrate was purified by reverse phase flash (C18 column, eluted with acetonitrile in H₂O, HCl condition) . The desired components were lyophilized to give the title compound 6 (15 mg, yield 27%) as a yellow solid. ¹H NMR (500 MHz, DMSO-d₆ (D₂O) ) δ 8.72 (d, J = 5.3 Hz, 1H) , 8.46 (s, 1H) , 7.95 (d, J = 11.2 Hz, 1H) , 7.63 (s, 1H) , 4.19 (dt, J = 11.5, 5.5 Hz, 2H) , 4.10 (dd, J = 11.1, 7.0 Hz, 1H) , 3.90 (d, J = 9.0 Hz, 1H) , 3.65 (d, J = 9.2 Hz, 1H) , 3.35 (d, J = 5.1 Hz, 1H) , 3.08 –2.98 (m, 2H) , 1.71 (d, J = 13.6 Hz, 8H) , 1.66 (d, J = 13.2 Hz, 1H) , 1.55 (d, J = 13.3 Hz, 1H) , 1.22 (d, J = 6.4 Hz, 3H) . LCMS: m/z calculated for C₂₀H₂₉N₆O₂PS: 448.53; found: 449.89 [M+H] ⁺.

Example 7.

Synthesis of (3- ( (5- ( (3S, 4S) -4-amino-3-methyl-2-oxa-8-azaspiro [4.5] decan-8-yl) pyrazin-2-yl) thio) -2-chlorophenyl) dimethylphosphine oxide (7)

Synthetic scheme 7

Step 1: Under nitrogen, a mixture of compound 7-1 (1.0 g, 5.17 mmol) , 2-Methoxyethanethiol 1-2 (750 mg, 6.25 mmol) , Xantphos (300 mg, 0.518 mmol) , DIEPA (1.3 g, 10.06 mmol) and palladium (II) acetate (62 mg, 0.276 mmol) in dioxane (5 mL) was stirred at 115℃ for 16 h. After completion of the reaction, the mixture was filtered. The filtrated was diluted with EtOAc (50 mL) and washed with water (50 mL) . The organic layer was dried over anhydrous sodium sulfate, filtered and concentrated. The residue was purified by silica gel column (eluted with 20%EtOAc in Petroleum ether) to afford the title compound 7-2 (860 mg, yield 71%) as a pale-yellow solid. ¹H NMR (400 MHz, Chloroform-d) δ 8.38 (d, J = 1.5 Hz, 1H) , 8.22 (d, J = 1.5 Hz, 1H) , 3.71 (s, 3H) , 3.41 (t, J = 7.0 Hz, 2H) , 2.76 (t, J = 7.0 Hz, 2H) .

Step 2: Under nitrogen, to a solution of compound 7-2 (860 mg, 3.70 mmol) in anhydrous THF (5mL) was added dropwise sodium ethoxide (20 w%solution in EtOH, 1.9 g, 5.59 mmol) at -30 ℃. The mixture was stirred at -30 ℃ for 1 h, then warmed at 25℃ and stirred for 2 h. After completion of the reaction, the mixture was concentrated under reduced pressure. The residue was dispersed in DCM (15 mL) and petroleum ether (15 mL) . The suspension was filtered and the solid was dried in vacuum to give the title compound 7-3 (780 mg, yield quantitively, 80 w%) as a yellow solid.

Step 3: Under nitrogen, a mixture of compound 7-4 (5 g, 24.2 mmol) , dimethylphosphine oxide 1-7 (2.9 g, 36.3 mmol) , Xantphos (1.4 g, 2.4 mmol) , K₃PO₄ (10.3 g, 48.4 mmol) and palladium (II) acetate (270 mg, 1.2 mmol) in DMF (50 mL) was stirred at 120℃for 16 h. After completion, the reaction was cooled and concentrated. The residue was purified by silica gel column (eluted with 10%MeOH in DCM) to afford the crude product, which was further purified by reverse phase flash (eluted with acetonitrile in H₂O, HCl condition) . The desired components were lyophilized to afford the title compound 7-5 (hydrochloride, 1.3 g, yield 22%) as a yellow solid. ¹H NMR (400 MHz, Chloroform-d) δ 8.16 (dd, J = 11.4, 7.7 Hz, 1H) , 8.09 (d, J = 7.8 Hz, 1H) , 7.19 (t, J = 7.4 Hz, 1H) , 3.54 (s, 2H) , 2.00 (d, J = 13.5 Hz, 6H) . LCMS: m/z calculated for C₈H₁₁ClNOP: 203.61; found: 204.62 [M+H] ⁺.

Step 4: To a solution of compound 7-5 (hydrochloride, 1.3 g, 5.41 mmol) in MeCN (15 ml) was added 4 N HCl (6 mL) . After stirring for 10 min at 0 ℃, NaNO₂ (480 mg, 6.95 mmol) in cold H₂O (5 mL) was added dropwise. After stirring for 30 min at 0 ℃, CuI (610 mg, 3.20 mmol) and KI (2.1 g, 12.6 mmol) was added thereto. The mixture was stirred at 0 ℃ for 4 h, water (50 mL) was added to quench the reaction. The resulted mixture extracted with EtOAc (50 mL x 2) . The combined organic layers were washed with saturated Na₂S₂O₃ and brine, dried over anhydrous Na₂SO₄, filtered and concentrated. The crude product was purified by silica gel column (eluted with 10%MeOH in DCM) to afford the title compound 7-6 (890 mg, yield 52%) as a white solid. ¹H NMR (400 MHz, Chloroform-d) δ 8.16 –8.08 (m, 2H) , 7.19 (t, J = 7.8 Hz, 1H) , 2.01 (s, 3H) , 1.97 (s, 3H) . LCMS: m/z calculated for C₈H₉ClIOP: 314.49; found: 315.49 [M+H] ⁺.

Step 5: Under nitrogen, a mixture of compound 7-6 (120 mg, 0.381 mmol) , compound 7-3 (80 w%, 96 mg, 0.457 mmol) , Xantphos (44 mg, 0.076 mmol) , Pd₂ (Dba) ₃ (35 mg, 0.0382 mmol) and DIPEA (150 mg, 1.16 mmol) in dioxane (2 mL) was stirred at 115℃ for 16 h. After completion of the reaction, the mixture was cooled and diluted with ethyl acetate (10 mL) and water (10 mL) . The organic layer was separated, dried over anhydrous sodium sulfate, filtered and concentrated. The residue was purified by reverse phase flash (C18 column, eluted with acetonitrile in H₂O, HCl condition) . The desired components were lyophilized to afford the title compound 7-7 (hydrochloride, 52 mg, yield 36 %) as a yellow solid. LCMS: m/z calculated for C₁₂H₁₁Cl₂N₂OPS: 333.17; found: 333.74 [M+H] ⁺.

Step 6: A mixture of compound 7-7 (hydrochloride, 50 mg, 0.135 mmol) , compound 1-11 (50 mg, 0.206 mmol) and K₂CO₃ (95 mg, 0.687 mmol) in N, N-dimethylacetamide (2 mL) was stirred at 120 ℃ for 24 h. After completion of the reaction, the mixture was cooled and filtered. The filtrated was purified by pre-HPLC (C18 column, eluted with acetonitrile in H₂O, HCl condition) . The desired component was lyophilized to give the title compound 7 (21 mg, yield 30%) as a yellow solid. ¹H NMR (400 MHz, DMSO-d₆ (D₂O) ) δ 8.46 (s, 1H) , 8.29 (s, 1H) , 7.77 –7.69 (m, 1H) , 7.39 (t, J = 8.5 Hz, 1H) , 7.06 (d, J = 7.9 Hz, 1H) , 4.21 (q, J = 15.4, 13.8 Hz, 3H) , 3.89 (d, J = 9.2 Hz, 1H) , 3.70 (d, J = 9.2 Hz, 1H) , 3.38 (d, J = 4.7 Hz, 1H) , 3.13 (d, J = 12.7 Hz, 2H) , 1.83 (s, 3H) , 1.80 (s, 3H) , 1.71 (d, J = 11.9 Hz, 3H) , 1.57 (d, J = 13.4 Hz, 1H) , 1.22 –1.18 (m, 3H) . LCMS: m/z calculated for C₂₁H₂₈ClN₄O₂PS: 466.96; found: 467.66 [M+H] ⁺.

Example 8.

Synthesis of (3- ( (3-amino-5- ( (3S, 4S) -4-amino-3-methyl-2-oxa-8-azaspiro [4.5] decan-8-yl) pyrazin-2-yl) thio) -2-chlorophenyl) dimethylphosphine oxide (8)

Synthetic scheme 8

Step 1: Under nitrogen, a mixture of compound 7-6 (155 mg, 0.493 mmol) , 2-Methoxyethanethiol 1-2 (90 mg, 0.75 mmol) , Xantphos (57 mg, 0.098 mmol) , palladium (II) acetate (12 mg, 0.049 mmol) and DIEPA (190 mg, 1.47 mmol) in dioxane (3 mL) was stirred at 115 ℃ for 16 h. After completion of the reaction, the mixture was filtered. The filtrated was diluted with EtOAc (20 mL) and washed with water (20 mL) . The organic layer was dried over anhydrous sodium sulfate, filtered and concentrated. The residue was purified by silica gel column (eluted with 5%MeOH in DCM) to afford the title compound --1 (120 mg, yield 79%) as a pale-yellow solid.

Step 2: Under nitrogen, to a solution of compound 8-1 (100 mg, 0.326 mmol) in anhydrous THF (3 mL) was added dropwise sodium ethoxide (20 w%solution in EtOH, 166 mg, 0.488 mmol) at 0 ℃. The mixture was stirred at room temperature for 1 h. After completion of the reaction, the mixture was concentrated under reduced pressure to give the title compound 8-2 (113 mg, yield quantitively, 70 w%) as a yellow solid, which was used at next step without purification.

Step 3: Under nitrogen, a mixture of compound 1-1 (68 mg, 0.326 mmol) , compound 8-2 (70 w%, 113 mg, 0.326 mmol) , Xantphos (37 mg, 0.064 mmol) , Pd₂ (Dba) ₃ (30 mg, 0.0327 mmol) and DIPEA (125 mg, 0.967 mmol) in dioxane (2 mL) was stirred at 115 ℃ for 16 h. After completion of the reaction, the mixture was cooled and concentrated. The residue was purified by reverse phase flash (C18 column, eluted with acetonitrile in H₂O, HCl condition) . The desired components were lyophilized to afford the title compound 8-3 (hydrochloride, 22 mg, yield 17 %) as a yellow solid.

Step 4: A mixture of compound 8-3 (hydrochloride, 22 mg, 0.057 mmol) , compound 1-11 (20 mg, 0.082 mmol) and K₂CO₃ (40 mg, 0.289 mmol) in N, N-dimethylacetamide (2 mL) was stirred at 120 ℃ for 16 h. After completion of the reaction, the mixture was cooled and filtered. The filtrated was purified by pre-HPLC (C18 column, eluted with acetonitrile in H₂O, HCl condition) . The desired component was lyophilized to give the title compound 8 (10 mg, yield 33%) as a yellow solid. ¹H NMR (400 MHz, DMSO-d₆ (D₂O) ) δ 7.73 –7.67 (m, 2H) , 7.40 (t, J = 7.8 Hz, 1H) , 6.81 (d, J = 7.5 Hz, 1H) , 4.27 –4.12 (m, 3H) , 3.91 (d, J = 9.1 Hz, 1H) , 3.72 (d, J = 9.4 Hz, 1H) , 3.41 (d, J = 4.8 Hz, 1H) , 3.06 (t, J = 11.4 Hz, 2H) , 1.86 (s, 3H) , 1.83 (s, 3H) , 1.73 (s, 3H) , 1.57 (d, J = 12.8 Hz, 1H) , 1.23 (d, J = 6.7 Hz, 3H) . LCMS: m/z calculated for C₂₁H₂₉ClN₅O₂PS: 481.98; found: 483.23 [M+H] ⁺.

Example 9.

Synthesis of (2- ( (5- ( (3S, 4S) -4-amino-3-methyl-2-oxa-8-azaspiro [4.5] decan-8-yl) pyrazin-2-yl) thio) -3-chloropyridin-4-yl) dimethylphosphine oxide (9)

Synthetic scheme 9

Step 1: Tert-butyl nitrite (6.1 g, 59.1 mmol) was added dropwise to a mixture of compound 9-1 (10 g, 39.3 mmol) and copper (I) bromide (8.5 g, 59.2 mmol) in anhydrous acetonitrile (100 mL) at room temperature under nitrogen atmosphere. The mixture was then heated to 75 ℃ and stirred for 15 h. After completion of the reaction, the mixture was cooled to room temperature and diluted with EtOAc (200 mL) . The resulted mixture was washed with water and brine. The organic layer was dried over anhydrous sodium sulfate, filtered and concentrated. The residue was purified by silica gel column chromatography (eluted with 10%EtOAc in petroleum ether) to afford the title compound 9-2 (2.4 g, yield 19%) as a white solid.

Step 2: Under nitrogen, a mixture of compound 9-2 (900 mg, 2.82 mmol) , compound 1-7 (265 mg, 3.40 mmol) , Xantphos (326 mg, 0.563 mmol) , Pd₂ (Dba) ₃ (258 mg, 0.282 mmol) and TEA (720 mg, 7.13 mmol) in dioxane (9 mL) was stirred at 120 ℃ for 16 h. After completion of the reaction, the mixture was concentrated. The residue was purified by silica gel column chromatography (eluted with 5%MeOH in dichloromethane) to give a crude product, which was further purified by reverse phase flash (C18 column, eluted with acetonitrile in H₂O, HCl condition) . The desired components were lyophilized to afford the title compound 9-3 (341 mg, yield 45%) as a yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 8.55 (dd, J = 4.5, 2.1 Hz, 1H) , 7.88 (dd, J = 11.0, 4.7 Hz, 1H) , 1.87 (s, 3H) , 1.84 (s, 3H) .

Step 3: Under nitrogen, a mixture of compound 9-3 (240 mg, 0.894 mmol) , compound 7-3 (80 w%, 280 mg, 1.33 mmol) , Xantphos (105 mg, 0.181 mmol) , Pd₂ (Dba) ₃ (80 mg, 0.087 mmol) and DIPEA (350 mg, 2.71 mmol) in dioxane (4 mL) was stirred at 105 ℃ for 16 h. After completion of the reaction, the mixture was concentrated. The residue was purified by silica gel column chromatography (eluted with 10%MeOH in dichloromethane) to give a crude product, which was further purified by reverse phase flash (C18 column, eluted with acetonitrile in H₂O, HCl condition) . The desired components were lyophilized to afford the title compound 9-4 (140 mg, yield 46%) as a yellow solid. LCMS: m/z calculated for C11H10Cl2N3OPS: 334.15; found: 298.54 [M-Cl] ⁺.

Step 4: A mixture of compound 9-4 (130 mg, 0.389 mmol) , compound 1-11 (140 mg, 0.576 mmol) and K₂CO₃ (270 mg, 1.95 mmol) in N, N-dimethylacetamide (3 mL) was stirred at 120 ℃ for 16 h. After completion of the reaction, the mixture was cooled and filtered. The filtrated was purified by pre-HPLC (C18 column, eluted with acetonitrile in H₂O, HCl condition) . The desired component was lyophilized to give the title compound 9 (45 mg, yield 23%) as a yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 8.41 –8.36 (m, 2H) , 8.24 (s, 1H) , 7.57 –7.51 (m, 1H) , 4.22 (dt, J = 18.7, 13.6 Hz, 3H) , 3.90 (dd, J = 9.0, 2.9 Hz, 1H) , 3.71 (s, 1H) , 3.37 (dd, J = 5.1, 2.8 Hz, 1H) , 3.19 –3.07 (m, 2H) , 1.86 (d, J = 3.0 Hz, 3H) , 1.83 (d, J = 2.9 Hz, 3H) , 1.75 (d, J = 10.2 Hz, 3H) , 1.59 (d, J = 13.0 Hz, 1H) , 1.21 (dd, J = 6.4, 3.2 Hz, 3H) . LCMS: m/z calculated for C₂₀H₂₇ClN₅O₂PS: 467.95; found: 468.89 [M+H] ⁺.

Example 10.

Synthesis of (4- ( (5- ( (3S, 4S) -4-amino-3-methyl-2-oxa-8-azaspiro [4.5] decan-8-yl) pyrazin-2-yl) thio) -3-chloropyridin-2-yl) dimethylphosphine oxide (10)

Synthetic scheme 10

Step 1: Under nitrogen, a mixture of compound 9-3 (1 g, 3.14 mmol) , compound 1-2 (400 mg, 3.32 mmol) , Xantphos (360 mg, 0.622 mmol) , Pd (OAc) 2 (71 mg, 0.316 mmol) and DIPEA (1.02 g, 7.89 mmol) in dioxane (10 mL) was stirred at 105 ℃ for 16 h. After completion of the reaction, the mixture was cooled, filtered and concentrated. The residue was purified by silica gel column chromatography (eluted with 20%EtOAc in petroleum ether) to afford the title compound 10-1 (880 mg, yield 90%) as a yellow solid. LCMS: m/z calculated for C₉H₉BrClNO₂S: 310.59; found: 222.49 [M- (methyl propionate) +H] ⁺.

Step 2: Under nitrogen, a mixture of compound 10-1 (1.18 g, 3.80 mmol) , compound 1-7 (600 mg, 7.69 mmol) , Xantphos (440 mg, 0.76 mmol) , Pd₂ (Dba) ₃ (350 mg, 0.382 mmol) and Et₃N (960 mg, 9.50 mmol) in dioxane (12 mL) was stirred at 120 ℃ for 2 h. The mixture was concentrated after cooled. The residue was purified by silica gel column chromatography (eluted with 10%MeOH in dichloromethane) to give a crude product, which was further purified by reverse phase flash (C18 column, eluted with acetonitrile in H₂O, TFA condition) . The desired components were lyophilized to afford the title compound 10-2 (290 mg, yield 24%) as a yellow solid. LCMS: m/z calculated for C₁₁H₁₅ClNO₃PS: 307.73; found: 308.63 [M+H] ⁺.

Step 3: Under nitrogen, to a solution of compound 10-2 (278 mg, 0.903 mmol) in anhydrous THF (5 mL) was added dropwise sodium ethoxide (20 w%solution in EtOH, 460 mg, 1.35 mmol) at -30 ℃. The mixture was stirred at -30 ℃ for 1 h. After completion of the reaction, the mixture was concentrated under reduced pressure. The residue was dispersed in DCM (15 mL) and petroleum ether (15 mL) . The suspension was filtered and the solid was dried in vacuum to give the title compound 10-3 (300 mg, yield quantitively, 73 w%) as a yellow solid.

Step 4: Under nitrogen, a mixture of compound 10-3 (73 w%, 113 mg, 0.338 mmol) , compound 7-1 (100 mg, 0.517 mmol) , Xantphos (40 mg, 0.069 mmol) , Pd₂ (Dba) ₃ (30 mg, 0.032 mmol) and DIPEA (130 mg, 1.0 mmol) in dioxane (3 mL) was stirred at 105 ℃ for 16 h. After completion of the reaction, the mixture was concentrated. The residue was purified by silica gel column chromatography (eluted with 10%MeOH in dichloromethane) to give a crude product, which was further purified by reverse phase flash (C18 column, eluted with acetonitrile in H₂O, HCl condition) . The desired components were lyophilized to afford the title compound 10-4 (60 mg, yield 53%) as a yellow solid. LCMS: m/z calculated for C₁₁H₁₀Cl₂N₃OPS: 334.15; found: 298.55 [M-Cl] ⁺.

Step 5: A mixture of compound 10-4 (55 mg, 0.164 mmol) , compound 1-11 (60 mg, 0.247 mmol) and K₂CO₃ (120 mg, 0.868 mmol) in N, N-dimethylacetamide (3 mL) was stirred at 120 ℃ for 16 h. After completion of the reaction, the mixture was cooled and filtered. The filtrated was purified by pre-HPLC (C18 column, eluted with acetonitrile in H₂O, TFA condition) . The desired component was lyophilized to give the title compound 10 (15 mg, yield 15%) as a yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 8.54 (s, 1H) , 8.41 –8.34 (m, 2H) , 6.85 (d, J = 4.9 Hz, 1H) , 4.29 (d, J = 13.7 Hz, 1H) , 4.21 (dd, J = 12.4, 5.7 Hz, 2H) , 3.91 (d, J = 9.1 Hz, 1H) , 3.70 (d, J = 9.1 Hz, 1H) , 3.40 (d, J = 4.9 Hz, 1H) , 3.18 (q, J = 12.1, 10.9 Hz, 2H) , 1.83 (s, 3H) , 1.80 (s, 3H) , 1.74 (t, J = 11.6 Hz, 3H) , 1.63 –1.56 (m, 1H) , 1.22 (d, J = 6.4 Hz, 3H) . LCMS: m/z calculated for C₂₀H₂₇ClN₅O₂PS: 467.95; found: 490.98 [M+Na] ⁺.

Example 11.

Synthesis of (5- ( (5- ( (3S, 4S) -4-amino-3-methyl-2-oxa-8-azaspiro [4.5] decan-8-yl) pyrazin-2-yl) thio) -4-chloropyridin-3-yl) dimethylphosphine oxide (11)

Synthetic scheme 11

Step 1: A mixture of compound 11-1 (4 g, 19.3 mmol) and aqueous HCl (4 N, 20 mL, 80 mmol) in acetonitrile (30 mL) was stirred at room temperature for 10 min before cooled to 0 ℃. To this was added dropwise a cold solution of NaNO₂ (1.47 g, 21.3 mmol) in H₂O (10 mL) . After stirring at 0 ℃ for 30 min, a cold solution of KI (6.4 g, 38.5 mmol) in H₂O (15 mL) was added thereto. The resulted mixture was stirred at 0 ℃ for 30 min and then warmed to room temperature with further stirring for 2 h. After completion of the reaction, the mixture was poured into H₂O (150 mL) and extracted with EtOAc (150 mL x 2) . The combined organic layers were washed with sat. NaHCO₃ solution and sat. Na₂S₂O₃ solution, followed by washing with water and brine. The resulted organic layer was dried over anhydrous sodium sulfate, filtered and concentrated. The residue was purified by silica gel column chromatography (eluted with 10%EtOAc in petroleum ether) to afford the title compound 11-2 (4.5 g, yield 73%) as a pale-yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 8.93 (s, 1H) , 8.79 (s, 1H) . LCMS: m/z calculated for C₅H₂BrClIN: 318.34; found: 318.51 [M+H] ⁺.

Step 2: Under nitrogen, a mixture of compound 11-2 (600 mg, 1.88 mmol) , compound 1-7 (176 mg, 2.25 mmol) , Xantphos (217 mg, 0.375 mmol) , Pd₂ (Dba) ₃ (170 mg, 0.185 mmol) and Et₃N (570 mg, 5.64 mmol) in dioxane (5 mL) was stirred at 100 ℃ for 2 h. The mixture was cooled and concentrated. The residue was purified by silica gel column chromatography (eluted with 10%MeOH in dichloromethane) to give a crude product, which was further purified by reverse phase flash (C18 column, eluted with acetonitrile in H₂O, HCl condition) . The desired components were lyophilized to afford the title compound 11-3 (410 mg, yield 81%) as a yellow solid. LCMS: m/z calculated for C₇H₈BrClNOP: 268.47; found: 268.63 [M+H] ⁺.

Step 3: Under nitrogen, a mixture of compound 11-3 (200 mg, 0.745 mmol) , compound 7-3 (80 w%, 235 mg, 1.11 mmol) , Xantphos (86 mg, 0.148 mmol) , Pd₂ (Dba) ₃ (68 mg, 0.074 mmol) and DIPEA (280 mg, 2.16 mmol) in dioxane (3 mL) was stirred at 105 ℃ for 16 h. The mixture was cooled and concentrated. The residue was purified by silica gel column chromatography (eluted with 20%MeOH in dichloromethane) to give a crude product, which was further purified by reverse phase flash (C18 column, eluted with acetonitrile in H₂O, HCl condition) . The desired components were lyophilized to afford the title compound 11-4 (100 mg, yield 40%) as a yellow solid. LCMS: m/z calculated for C₁₁H₁₀Cl₂N₃OPS: 334.15; found: 298.57 [M-Cl] ⁺.

Step 4: A mixture of compound 11-4 (61 mg, 0.182 mmol) , compound 1-11 (66 mg, 0.271 mmol) and K₂CO₃ (125 mg, 0.904 mmol) in N, N-dimethylacetamide (3 mL) was stirred at 120 ℃ for 16 h. After completion of the reaction, the mixture was cooled and filtered. The filtrated was purified by pre-HPLC (C18 column, eluted with acetonitrile in H₂O, TFA condition) . The desired component was lyophilized to give the title compound 11 (20 mg, yield 18%) as a yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 8.97 –8.90 (m, 2H) , 8.20 (d, J = 1.5 Hz, 1H) , 8.17 (d, J = 1.5 Hz, 1H) , 4.22 –4.16 (m, 1H) , 4.12 (d, J = 13.6 Hz, 1H) , 4.05 (d, J = 14.3 Hz, 1H) , 3.85 (d, J = 9.2 Hz, 1H) , 3.67 (d, J = 9.1 Hz, 1H) , 3.36 (d, J = 4.8 Hz, 1H) , 3.10 – 2.98 (m, 2H) , 1.92 (s, 3H) , 1.89 (s, 3H) , 1.71 –1.65 (m, 3H) , 1.54 (d, J = 13.6 Hz, 1H) , 1.19 (d, J = 6.5 Hz, 3H) . LCMS: m/z calculated for C₂₀H₂₇ClN₅O₂PS: 467.95; found: 432.67 [M-Cl] ⁺.

Example 12.

Synthesis of (4- ( (3-amino-5- ( (3S, 4S) -4-amino-3-methyl-2-oxa-8-azaspiro [4.5] decan-8-yl) pyrazin-2-yl) thio) -3-chloropyridin-2-yl) dimethylphosphine oxide (12)

Synthetic scheme 12

Step 1: Under nitrogen, a mixture of compound 10-3 (73 w%, 200 mg, 0.60 mmol) , compound 1-1 (0.911 mmol) , Xantphos (70 mg, 0.121 mmol) , Pd₂ (Dba) ₃ (55 mg, 0.06 mmol) and DIPEA (235 mg, 1.82 mmol) in dioxane (3 mL) was stirred at 105 ℃ for 16 h. The mixture was cooled and concentrated. The residue was purified by silica gel column chromatography (eluted with 20%MeOH in dichloromethane) to give a crude product, which was further purified by reverse phase flash (C18 column, eluted with acetonitrile in H₂O, HCl condition) . The desired components were lyophilized to afford the title compound 12-1 (hydrochloride, 119 mg, yield 51%) as a yellow solid. LCMS: m/z calculated for C₁₁H₁₁Cl₂N₄OPS: 349.17; found: 313.68 [M-Cl] ⁺.

Step 2: A mixture of compound 12-1 (155 mg, 0.402 mmol) , compound 1-11 (145 mg, 0.596 mmol) and K₂CO₃ (280 mg, 2.02 mmol) in N, N-dimethylacetamide (4 mL) was stirred at 120 ℃ for 16 h. After completion of the reaction, the mixture was cooled and filtered. The filtrated was purified by pre-HPLC (C18 column, eluted with acetonitrile in H₂O, TFA condition) . The desired component was lyophilized to give the title compound 12 (50 mg, yield 20%) as a pale-yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 8.35 (d, J = 5.2 Hz, 1H) , 7.68 (s, 1H) , 6.66 (dd, J = 5.3, 1.4 Hz, 1H) , 4.25 –4.11 (m, 3H) , 3.88 (d, J = 9.1 Hz, 1H) , 3.69 (d, J = 9.1 Hz, 1H) , 3.36 (d, J = 4.9 Hz, 1H) , 3.11 –2.99 (m, 2H) , 1.82 (s, 3H) , 1.79 (s, 3H) , 1.70 (d, J = 7.1 Hz, 3H) , 1.54 (d, J = 13.1 Hz, 1H) , 1.20 (d, J = 6.7 Hz, 3H) . LCMS: m/z calculated for C₂₀H₂₈ClN₆O₂PS: 482.97; found: 505.66 [M+Na] ⁺.

Example 13.

Synthesis of (2- ( (3-amino-5- ( (3S, 4S) -4-amino-3-methyl-2-oxa-8-azaspiro [4.5] decan-8-yl) pyrazin-2-yl) thio) -3-chloropyridin-4-yl) dimethylphosphine oxide (13)

Synthetic scheme 13

Step 1: Under nitrogen atmosphere, to a solution of compound 1-3 (5 g, 20.2 mmol) in anhydrous THF (8 mL) was added dropwise NaOEt (20 w%solution in EtOH, 11 g, 32.3 mmol) at -30 ℃. The mixture was stirred at -30 ℃ for 2 h. After completion of the reaction, the mixture was warmed to room temperature and concentrated. The residue was dispersed in DCM (30 mL) and petroleum ether (30 mL) . The suspension was filtered and the solid was dried in vacumm to give the title compound 13-1 (4.64 g, yield quantitively, 80 w%) as a yellow solid.

Step 2: Under nitrogen, a mixture of compound 9-3 (260 mg, 0.968 mmol) , compound 13-1 (80 w%, 330 mg, 1.44 mmol) , Xantphos (115 mg, 0.199 mmol) , Pd₂ (Dba) ₃ (88 mg, 0.096 mmol) and DIPEA (375 mg, 2.90 mmol) in dioxane (4 mL) was stirred at 105 ℃ for 16 h. After completion of the reaction, the mixture was concentrated. The residue was purified by silica gel column chromatography (eluted with 10%MeOH in dichloromethane) to give a crude product, which was further purified by reverse phase flash (C18 column, eluted with acetonitrile in H₂O, HCl condition) . The desired components were lyophilized to afford the title compound 13-2 (hydrochloride, 150 mg, yield 40%) as a yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 8.42 (dd, J = 4.8, 2.3 Hz, 1H) , 7.84 (s, 1H) , 7.58 (dd, J = 11.1, 4.8 Hz, 1H) , 7.09 (s, 2H) , 1.87 (s, 3H) , 1.83 (s, 3H) . LCMS: m/z calculated for C₁₁H₁₁Cl₂N₄OPS: 349.17; found: 313.57 [M-Cl] ⁺.

Step 3: A mixture of compound 13-2 (hydrochloride, 150 mg, 0.389 mmol) , compound 1-11 (140 mg, 0.576 mmol) and K₂CO₃ (270 mg, 1.95 mmol) in N, N-dimethylacetamide (3 mL) was stirred at 120 ℃ for 16 h. After completion of the reaction, the mixture was cooled and filtered. The filtrated was purified by pre-HPLC (C18 column, eluted with acetonitrile in H₂O, AcOH condition) . The desired component was lyophilized to give the title compound 13 (30 mg, yield 14%) as a brown solid. ¹H NMR (400 MHz, DMSO-d₆) δ 8.37 (d, J = 2.5 Hz, 1H) , 7.53 –7.45 (m, 2H) , 4.12 –4.01 (m, 1H) , 3.85 (d, J = 4.6 Hz, 2H) , 3.67 (s, 1H) , 3.51 (d, J = 8.6 Hz, 1H) , 3.24 (d, J = 30.9 Hz, 2H) , 2.91 (d, J = 5.0 Hz, 1H) , 1.86 (s, 3H) , 1.82 (s, 3H) , 1.64 (d, J = 30.4 Hz, 2H) , 1.55 –1.43 (m, 2H) , 1.07 (d, J = 6.3 Hz, 3H) . LCMS: m/z calculated for C₂₀H₂₈ClN₆O₂PS: 482.97; found: 483.85 [M+H] ⁺.

Example 14.

Synthesis of (3- ( (3-amino-5- ( (3S, 4S) -4-amino-3-methyl-2-oxa-8-azaspiro [4.5] decan-8-yl) pyrazin-2-yl) thio) -2, 4, 6-trichlorophenyl) dimethylphosphine oxide (14)

Synthetic scheme 14

Step 1: To a solution of compound 14-1 (50.0 g, 362 mmol) in DMF (700 mL) was added NCS (48.5 g, 362 mmol) slowly at 0-5 ℃. The mixture was stirred at 75 ℃ for 16 h. After completion of the reaction, the mixture was cooled and partitioned between EtOAc (800 mL x 3) and water (2.1 L) . The organic layers were washed with brine (600 mL x 4) , dried over anhydrous Na₂SO₄ and concentrated. The crude product was purified by silica gel column (eluted with 3%EtOAc in petroleum ether) to afford the title compound 14-2 (37.4 g, yield 60%) as a yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 7.21 (t, J = 8.0 Hz, 1H) , 7.07 (dd, J = 7.7, 1.4 Hz, 1H) , 7.03 (dd, J = 8.2, 1.4 Hz, 1H) , 6.05 (s, 2H) .

Step 2: A mixture of compound 14-2 (24.6 g, 142 mmol) and conc. HCl (47.4 mL, 568.8 mmol) in MeCN (200 ml) and H₂O (300 mL) was stirred at 0 ℃ for 0.5 h. Then NaNO₂ (10.8 g, 156.4 mmol) in H₂O (50 mL) was added dropwise to the above mixture while keeping the temperature below 5 ℃. After stirring for 1 h, KI (47.2 g, 284 mmol) was added to the above mixture. After the reaction mixture was stirred at 0℃ for 1 h, the resulted mixture was extracted with EtOAc (300 mL x 3) . The combined organic layers were washed with sat. Na₂S₂O₃ solution_, dried over anhydrous Na₂SO₄ and concentrated under reduced pressure. The residue was purified by silica gel column (eluted with 5%EtOAc in petroleum ether) to afford the title compound 14-3 (25.1 g, yield 62%) as a light-yellow solid.

Step 3: A mixture of compound 14-3 (25.0 g, 88.3 mmol) , Fe powder (24.7 g, 441.5 mmol) and NH₄Cl (46.8 g, 883 mmol) in EtOH (300 ml) and H₂O (300 mL) was stirred at 100 ℃ for 3 h. After completion of the reaction, the hot mixture was filtered. The filtrate was cooled, concentrated and extracted with EtOAc (300 mL x 3) and H₂O (300 mL) . The combined organic layers were dried over anhydrous Na₂SO₄ and concentrated under reduced pressure. The residue was purified by silica gel column (eluted with 5%EtOAc in petroleum ether) to afford the title compound 14-4 (20.4 g, yield 89%) as a light-yellow solid. LCMS: m/z calculated for C₆H₅ClIN: 253.47; found: 127.37 [M-I] ⁺.

Step 4: A mixture of compound 14-4 (10.0 g, 39.5 mmol) , compound 2-3 (9.2 g, 118 mmol) , Pd (OAc) ₂ (889 mg, 4.0 mmol) , XantPhos (4.6 g, 7.9 mmol) and K₃PO₄ (16.7g, 79.0 mmol) in DMF (300 mL) was stirred at 120 ℃ for 16 h. After completion of the reaction, the mixture was cooled to room temperature, filtered and concentrated under vacuum. The residue was purified by silica gel column (eluted with 3%MeOH in DCM) to afford the crude product, which was furtherly purified by reverse phase flash chromatography (C18 column, eluted with MeCN in water, HCl condition) . The desired components were lyophilized to afford the title compound 7-5 (3.7 g, yield 46%) as a light-yellow solid. LCMS: m/z calculated for C₈H₁₁ClNOP: 203.61; found: 168.61 [M-Cl] ⁺.

Step 5: To a mixture of compound 7-5 (2.3 g, 11.3 mmol) in DMF (38.0 mL) was added NCS (1.5 g, 11.3 mmol) at 0-5 ℃. Then it was stirred at room temperature for 1 h. After completion of the reaction, the mixture was concentrated under vacuum. Then the crude product was purified by reverse phase flash chromatography (C18 column, eluted with MeCN in water, HCl condition) . The desired components were lyophilized respectively to afford the title compound 14-5 (980 mg, yield 32%) as a brown oil and a by-product 33-6 (550 mg, yield 21%) as a light-yellow oil. LCMS: m/z calculated for C₈H₉Cl₃NOP (33-5) : 272.49; found: 272.6 [M+H] ⁺. LCMS: m/z calculated for C₈H₁₀Cl₂NOP (33-6) : 238.05; found: 238.72 [M+H] ⁺.

Step 6: A mixture of compound 14-5 (900 mg, 3.3 mmol) and conc. HCl (1.1 ml, 13.2 mmol) in MeCN (10 mL) and H₂O (15 mL) was stirred at 0 ℃ for 0.5 h. To this was added dropwise a solution of NaNO₂ (250.5 mg, 3.6 mmol) in H₂O (2.5 mL) while keeping the temperature below 5 ℃. After stirring for 1 h, KI (1.1 g, 6.6 mmol) was added to the above mixture. After the reaction was stirred at 0 ℃ for 1 h, the resulted mixture was extracted with EtOAc (30 mL x3) . The combined organic layers were washed with sat. Na₂S₂O₃ solution, dried over anhydrous Na₂SO₄ and concentrated under reduced pressure. The residue was purified by silica gel column (eluted with 5%MeOH in DCM) to afford the title compound 14-7 (848 mg, yield 67%) as a yellow solid. LCMS: m/z calculated for C₈H₇Cl₃IOP: 383.37; found: 383.68. [M+H] ⁺.

Step 7: A mixture of compound 14-7 (600 mg, 1.6 mmol) , compound 14-8 (236 mg, 1.8 mmol) , Pd₂ (dba) ₃ (147 mg, 0.2 mmol) , XantPhos (185 mg, 0.3 mmol) and DIPEA (619 mg, 4.8 mmol) in dioxane (10 mL) was stirred at 100 ℃ for 3 h. After completion of the reaction, the mixture was cooled to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 3%MeOH in DCM) to afford the crude product, which was furtherly purified by reverse phase flash chromatography (C18 column, eluted with MeCN in water, HCl condition) . The desired components were lyophilized to afford the title compound 14-9 (330 mg, yield 54%) as a yellow oil. LCMS: m/z calculated for C₁₃H₁₆Cl₃O₃PS: 389.65; found: 389.38 [M+H] ⁺.

Step 8: To a mixture of compound 14-9 (330 mg, 0.8 mmol) in THF (4 mL) was added EtONa (20%in EtOH, 408 mg, 1.2 mmol) dropwise at -30 ℃. Then it was stirred at -30 ℃ for 1 h. After completion of the reaction, the mixture was concentrated under vacuum. Then the crude product was washed with 40 mL of cold solvent (petroleum ether/DCM=1: 1) , filtered and dried under vacuum to afford the title compound 14-10 (71 w%, 370 mg, yield quantitively) as a yellow solid. LCMS: m/z calculated for C₈H₇Cl₃NaOPS: 311.52; found: 289.42 [M-Na+H] ⁺.

Step 9: A mixture of compound 14-10 (71 w%, 350 mg, 0.798 mmol) , compound 1-1 (274 mg, 1.3 mmol) , Pd₂ (dba) ₃ (100 mg, 0.1 mmol) , XantPhos (116 mg, 0.2 mmol) and DIPEA (426 mg, 3.3 mmol) in dioxane (8 mL) was stirred at 105℃ for 2 h. The mixture was cooled to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 3%MeOH in DCM) to afford the crude product, which was furtherly purified by reverse phase flash chromatography (C18 column, eluted with MeCN in water, HCl condition) . The desired components were lyophilized to afford the title compound 14-11 (85 mg, yield 24%) as an orange solid. LCMS: m/z calculated for C₁₂H₁₀Cl₄N₃OPS: 417.07; found: 380.39 [M-Cl] ⁺.

Step 10: To a mixture of compound 14-11 (80 mg, 0.192 mmol) and K₂CO₃ (138 mg, 1.0 mmol) in DMAc (1 mL) was added compound 1-11 (49 mg, 0.2 mmol) at room temperature. Then it was stirred at 100 ℃ for 4 h. After completion of the reaction, the mixture was cooled to room temperature and filtered. The resulted mixture was purified by reverse phase flash chromatography (C18 column, eluted with MeCN in water, HCl condition) . The desired components were lyophilized to afford the title compound 14 (12.5 mg, yield 12%) as a light-yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 7.82 (s, 1H) , 7.53 (s, 1H) , 4.19 (s, 1H) , 3.86 (s, 1H) , 3.69 (d, J = 16.8 Hz, 2H) , 3.43 –3.26 (m, 3H) , 2.96 (s, 1H) , 1.97 (d, J = 13.6 Hz, 8H) , 1.79 (s, 1H) , 1.62 (d, J = 12.8 Hz, 1H) , 1.21 (d, J = 6.5 Hz, 3H) . LCMS: m/z calculated for C₂₁H₂₇Cl₃N₅O₂PS: 550.86; found: 550.9 [M+H] ⁺.

Example 15.

Synthesis of (3- ( (3-amino-5- ( (3S, 4S) -4-amino-3-methyl-2-oxa-8-azaspiro [4.5] decan-8-yl) pyrazin-2-yl) thio) -2, 4, 6-trichlorophenyl) dimethylphosphine oxide (15)

Synthetic scheme 15

Step 1: A mixture of compound 14-6 (550 mg, 2.3 mmol) and conc. HCl (0.8 ml, 9.2 mmol) in MeCN (5 ml) and H₂O (10 mL) was stirred at 0 ℃ for 0.5 h. Then NaNO₂ (174 mg, 2.5 mmol) in H₂O (1.5 ml) was added dropwise to the above mixture while keeping the temperature below 5 ℃. After stirred for 1 h, KI (763 mg, 4.6 mmol) was added to the above mixture while keeping the temperature below 5 ℃. The mixture was stirred at 0 ℃ for 1 h. The resulted mixture was extracted with EtOAc (15 mL x3) . The combined organic layers were washed with sat. Na₂S₂O₃ solution, dried over anhydrous Na₂SO₄ and concentrated under reduced pressure. The residue was purified by silica gel column (eluted with 5%MeOH in DCM) to afford the title compound 15-1 (495 mg, yield 62%) as a yellow solid. LCMS: m/z calculated for C₈H₈Cl₂IOP: 348.93; found: 349.3 [M+H] ⁺.

Step 2: A mixture of compound 15-1 (383 mg, 1.1 mmol) , compound 14-8 (295 mg, 2.2 mmol) , Pd₂ (dba) ₃ (92 mg, 0.1 mmol) , XantPhos (115 mg, 0.2 mmol) and DIPEA (426 mg, 3.3 mmol) in dioxane (10 mL) was stirred at 105 ℃ for 16 h. After completion of the reaction, the mixture cooled to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 3%MeOH in DCM) to afford the title compound 15-2 (351 mg, yield 90%) as a yellow oil. LCMS: m/z calculated for C₁₃H₁₇Cl₂O₃PS: 355.21; found: 355.49 [M+H] ⁺.

Step 3: To a mixture of compound 15-2 (351 mg, 1.0 mmol) in THF (4.0 mL) was added dropwise EtONa (20%in EtOH, 500 mg, 1.47 mmol) at -30 ℃. Then it was stirred at -30 ℃ for 1 h. After completion of the reaction, the mixture was concentrated under vacuum. Then the crude product was washed with 40 mL of cold solvent (petroleum ether/DCM=1: 1) . After filtration, the solid was concentrated under vacuum to afford the title compound 15-3 (65 w%, 420 mg, yield quantitively) as a yellow solid. LCMS: m/z calculated for C₈H₈Cl₂NaOPS: 277.07; found: 255.46 [M-Na+H] ⁺.

Step 4: A mixture of compound 15-3 (65 w%, 300 mg, 0.70 mmol) , compound 1-1 (354 mg, 1.7 mmol) , Pd₂ (dba) ₃ (100.8 mg, 0.1 mmol) , XantPhos (115.8 mg, 0.2 mmol) and DIPEA (425.7 mg, 3.3 mmol) in dioxane (8.0 mL) was stirred at 105 ℃ for 2 h. After completion of the reaction, the mixture cooled to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 2%MeOH in DCM) to afford the crude product, which was further purified by reverse phase flash chromatography (C18 column, eluted with MeCN in water, HCl condition) . The desired components were lyophilized to afford the title compound 15-4 (117 mg, yield 40%) as an orange solid. LCMS: m/z calculated for C₁₂H₁₁Cl₃N₃OPS: 382.62; found: 382.58 [M+H] ⁺.

Step 5: To a mixture of compound 15-4 (80 mg, 0.2 mmol) and K₂CO₃ (138 mg, 1.0 mmol) in DMAc (1 mL) was added compound 1-11 (49 mg, 0.2 mmol) at room temperature. Then it was stirred at 120 ℃ for 6 h. After completion of the reaction, the mixture was cooled to room temperature. The crude product was purified by reverse phase flash chromatography (C18 column, eluted with MeCN in water, TFA condition) . The desired components were lyophilized to afford the title compound 15 (9.8 mg, yield 9%) as a light-yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 7.65 (s, 1H) , 7.38 (dd, J = 8.6, 3.2 Hz, 1H) , 6.69 (d, J = 8.7 Hz, 1H) , 4.23 –4.08 (m, 3H) , 3.87 (d, J = 9.1 Hz, 1H) , 3.70 (s, 1H) , 3.35 (d, J = 4.8 Hz, 1H) , 3.08 –2.97 (m, 2H) , 1.99 (s, 3H) , 1.95 (s, 3H) , 1.67 (d, J = 20.2 Hz, 3H) , 1.54 (d, J = 13.1 Hz, 1H) , 1.20 (d, J = 6.6 Hz, 3H) . LCMS: m/z calculated for C₂₁H₂₈Cl₂N₅O₂PS: 516.42; found: 516.7 [M+H] ⁺.

Example 16.

Synthesis of (3- ( (3-amino-5- (4-amino-4-methylpiperidin-1-yl) pyrazin-2-yl) thio) -2-chlorophenyl) diisopropylphosphine oxide (16)

Synthetic scheme 16

Step 1: Under nitrogen atmosphere, a solution of compound 16-1 (10 g, 72.4 mmol) in anhydrous THF (100 mL) was added dropwise to a flask containing isopropylmagnesium bromide (2.8 M, 77 mL, 215.6 mmol) with stirring at -20 ℃. The mixture was maintained at -20 ℃ for 15 min after addition, then allowed to warm slowly to room temperature and stirred for a further 20 h. After completion of the reaction, the mixture was quenched with 1 N HCl. The resulted mixture was extracted with MTBE and EtOAc. The organic layers were combined, dried over anhydrous Na2SO4, filtered and concentrated. The crude product was purified by silica gel column (eluted with 6%MeOH in DCM) to afford the title compound 16-2 (6.6 g, yield 67%) as a pale-yellow oil.

Step 2: A mixture of compound 7-4 (10.0 g, 48.5 mmol) and conc. HCl (16 mL, 192 mmol) in MeCN (100 mL) and H₂O (200 mL) was stirred at 0 ℃ for 0.5 h. To this was added dropwise a solution of NaNO₂ (4.0 g, 58.2 mmol) in H₂O (20 mL) while keeping the temperature below 5 ℃. After stirring for 1 h, KI (16.1 g, 97.0 mmol) was added to the above mixture. The reaction was furtherly stirred at 0 ℃ for 1 h. The resulted mixture was extracted with EtOAc (300 mL x3) . The combined organic layers were washed with sat. Na₂S₂O₃ solution_, dried over anhydrous Na₂SO₄ and concentrated under reduced pressure. The residue was purified by silica gel column (eluted with 100%petroleum ether) to afford the title compound 16-3 (13.1 g, yield 86%) as a white solid. ¹H NMR (400 MHz, DMSO-d₆) δ 7.97 (d, J = 7.9 Hz, 1H) , 7.79 (d, J = 8.0 Hz, 1H) , 7.00 (t, J = 7.9 Hz, 1H) .

Step 3: A mixture of compound 16-3 (5 g, 15.8 mmol) , compound 13-1 (2.9 g, 15.8 mmol) , Pd₂ (dba) ₃ (732.8 mg, 0.8 mmol) , XantPhos (1.9 g, 3.2 mmol) and DIPEA (6.1 g, 47.4 mmol) in dioxane (80 mL) was stirred at 100 ℃ for 16 h. After completion of the reaction, the mixture was cooled to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 5%EtOAc in petroleum ether) to afford the title compound 16-4 (4.4 g, yield 80%) as a brown solid. LCMS: m/z calculated for C₁₀H₆BrCl₂N₃S: 351.04; found: 352.5 [M+H] ⁺.

Step 4: A mixture of compound 16-4 (614 mg, 1.7 mmol) , K₂CO₃ (704 mg, 5.1 mmol) and compound 16-5 (437 mg, 2.0 mmol) in DMAc (10 ml) was stirred at 100℃ for 3 h. After completion of the reaction, the mixture was filtered. The filtrate was concentrated and extracted with EtOAc (3x30 ml) and H₂O (100 mL) . The combined organic layers were washed with brine, dried over anhydrous Na₂SO₄ and concentrated under reduced pressure. The residue was purified by silica gel column (eluted with 20%EtOAc in petroleum ether) to afford the title compound 16-6 (642 mg, yield 69%) as a light-yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 7.63 (s, 1H) , 7.52 (dd, J = 7.9, 1.5 Hz, 1H) , 7.15 –7.10 (m, 1H) , 6.61 –6.58 (m, 1H) , 6.14 (s, 2H) , 3.85 (d, J = 13.6 Hz, 2H) , 3.23 (t, J = 12.1 Hz, 2H) , 2.08 (d, J = 13.3 Hz, 2H) , 1.47 –1.41 (m, 2H) , 1.39 (s, 9H) , 1.25 (s, 3H) . LCMS: m/z calculated for C₂₁H₂₇BrClN₅O₂S: 528.89; found: 528.7 [M+H] ⁺.

Step 5: A mixture of compound 16-6 (500 mg, 0.9 mmol) , compound 16-2 (386 mg, 2.7 mmol) , Pd₂ (dba) ₃ (83 mg, 0.09 mmol) , XantPhos (116 mg, 0.2 mmol) and Et₃N (273 mg, 3 mmol) in dioxane (5 mL) was stirred at 100 ℃ for 16 h. After completion of the reaction, the mixture was cooled to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 10%MeOH in DCM) to afford the crude product, which was purified by reverse phase flash chromatography (C18 column, eluted with MeCN in water, TFA condition) . The desired components were lyophilized to afford the title compound 16 (5.5 mg, yield 1%) as a light-yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 7.71 –7.61 (m, 2H) , 7.36 (t, J = 7.8 Hz, 1H) , 6.79 (d, J = 8.1 Hz, 1H) , 4.03 (d, J = 13.6 Hz, 2H) , 3.26 (dt, J = 14.3, 6.8 Hz, 2H) , 2.58 (td, J = 7.1, 4.4 Hz, 2H) , 1.71 (d, J = 5.2 Hz, 4H) , 1.36 (s, 3H) , 1.21 (dd, J = 15.2, 7.1 Hz, 6H) , 0.84 (dd, J = 16.6, 7.2 Hz, 6H) . LCMS: m/z calculated for C₂₂H₃₃ClN₅OPS: 482.02; found: 482.47 [M+H] ⁺.

Example 17.

Synthesis of (3- ( (5- (4-amino-4-methylpiperidin-1-yl) pyrazin-2-yl) thio) -2-chlorophenyl) dimethylphosphine oxide (17)

Synthetic scheme 17

Step 1: A mixture of compound 7-7 (110 mg, 0.330 mmol) , K₂CO₃ (115 mg, 0.832 mmol) and compound 16-5 (110 mg, 0.514 mmol) in DMAc (4 mL) and H₂O (0.4 mL) was stirred at 100℃ for 3 h. After completion of the reaction, the mixture was extracted with EtOAc (3 x 30 mL) and H₂O (30 mL) . The combined organic layers were washed with brine, dried over anhydrous Na₂SO₄ and concentrated under reduced pressure. The residue was purified by silica gel column (eluted with 5%MeOH in dichloromethane) to afford the title compound 17-1 (150 mg, yield 89%) as a yellow solid. LCMS: m/z calculated for C₂₃H₃₂ClN₄O₃PS: 511.02; found: 511.77 [M+H] ⁺.

Step 2: To a solution of compound 17-1 (150 mg, 0.293 mmol) in dichloromethane (4 mL) was added TFA (2 mL) . Then the mixture was stirred at room temperature for 1 h. After completion of the reaction, the mixture was concentrated under vacuum. The residue was purified by pre-HPLC (C18 column, eluted with acetonitrile in water, TFA condition) . The desired components were lyophilized to afford the title compound 17 (47 mg, yield 38%) as a yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 8.46 (d, J = 1.4 Hz, 1H) , 8.30 (d, J = 1.3 Hz, 1H) , 7.74 (ddd, J = 11.8, 7.6, 1.6 Hz, 1H) , 7.40 (td, J = 7.8, 1.7 Hz, 1H) , 7.08 (dd, J = 8.0, 1.5 Hz, 1H) , 4.10 (dt, J = 14.0, 4.8 Hz, 2H) , 3.40 (ddd, J = 13.7, 8.4, 5.1 Hz, 2H) , 1.84 (s, 3H) , 1.80 (s, 3H) , 1.77 (q, J = 4.4 Hz, 4H) , 1.39 (s, 3H) . LCMS: m/z calculated for C₁₈H₂₄ClN₄OPS: 410.90; found: 411.74 [M+H] ⁺.

Example 18.

Synthesis of (S) - (3- ( (5- (1-amino-1, 3-dihydrospiro [indene-2, 4'-piperidin] -1'-yl) pyrazin-2-yl) thio) -2-chlorophenyl) dimethylphosphine oxide (18)

Synthetic scheme 18

Step 1: A mixture of compound 7-7 (140 mg, 0.420 mmol) , K₂CO₃ (290 mg, 2.10 mmol) and compound 18-1 (140 mg, 0.508 mmol) in DMAc (3 mL) was stirred at 100℃ for 16 h. After completion of the reaction, the mixture was cooled, diluted with EtOAc and concentrated. The residue was purified by pre-HPLC (C18 column, eluted with MeCN in water, TFA condition) . The desired components were lyophilized to afford the title compound 17 (50 mg, yield 23%) as a yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 8.47 (d, J = 1.5 Hz, 1H) , 8.29 (d, J = 1.3 Hz, 1H) , 7.73 (ddd, J = 11.8, 7.6, 1.6 Hz, 1H) , 7.54 (d, J = 7.1 Hz, 1H) , 7.41 (td, J = 7.8, 1.7 Hz, 1H) , 7.35 (d, J = 7.7 Hz, 2H) , 7.29 (td, J = 6.9, 6.3, 2.4 Hz, 1H) , 7.08 (dd, J = 8.0, 1.5 Hz, 1H) , 4.36 (d, J = 5.3 Hz, 2H) , 4.26 (d, J = 13.7 Hz, 1H) , 3.29 –3.16 (m, 3H) , 3.02 (d, J = 16.3 Hz, 1H) , 1.84 (s, 3H) , 1.81 (s, 3H) , 1.79 –1.70 (m, 2H) , 1.60 –1.49 (m, 2H) . LCMS: m/z calculated for C₂₅H₂₈ClN₄OPS: 499.01; found: 499.69 [M+H] ⁺.

Example 19.

Synthesis of (3- (3-amino-5- ( (3S, 4S) -4-amino-3-methyl-2-oxa-8-azaspiro [4.5] decan-8-yl) pyrazin-2-yl) -2-chlorophenyl) dimethylphosphine oxide (19)

Synthetic scheme 19

Step 1: Under nitrogen atmosphere, a mixture of compound 16-3 (10 g, 31.5 mmol) , compound 1-7 (5.0 g, 64.1 mmol) , Pd₂ (Dba) ₃ (1.44 g, 1.57 mmol) , XantPhos (1.83 g, 3.16 mmol) and DIPEA (12.2 g, 94.4 mmol) in dioxane (100 mL) was stirred at 120 ℃ for 16 h. After completion of the reaction, the mixture was cooled to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 5%EtOAc in petroleum ether) to afford the title compound 19-1 (6.32 g, yield 75%) as a yellow oil. LCMS: m/z calculated for C₈H₉BrClOP: 267.49; found: 267.53 [M+H] ⁺.

Step 2: Under nitrogen atmosphere, a mixture of compound 19-1 (1.9 g, 7.10 mmol) , bis (pinacolato) diboron (3.25 g, 12.8 mmol) , K₂OAc (2.09 g, 21.3 mmol) and Pd (dppf) Cl₂ (258 mg, 0.355 mmol) in dioxane (20 ml) was stirred at 90 ℃ for 16 h. After completion of the reaction, the mixture was cooled, diluted with EtOAc and filtered over a short silica gel column. The filtrate was concentrated and re-dissolved in dichloromethane (30 mL) and treated with 2 N HCl (30 mL) . The resulted mixture was stirred vigorously at room temperature for 24 h. After hydrolysis was completed, the mixture was diluted with dichloromethane and separated. The aqueous layer was purified by reverse phase flash chromatography (C18 column, eluted with MeCN in water, TFA condition) . The desired components were lyophilized to afford the title compound 19-2 (1.5 mg, yield 90%) as a pale-yellow oil. LCMS: m/z calculated for C₈H₁₁BClO₃P: 232.41; found: 233.57 [M+H] ⁺.

Step 3: Under nitrogen atmosphere, a mixture of compound 19-2 (520 mg, 2.23 mmol) , compound 1-1 (600 mg, 2.88 mmol) , Pd (dppf) Cl₂ (162 mg, 0.223 mmol) and K₃PO₄ (1.42 g, 6.69 mmol) in dioxane (8 mL) and H₂O (0.8 mL) was stirred at 90 ℃ for 1 h. After completion of the reaction, the mixture was cooled to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 10%MeOH in DCM) to afford the title compound 19-3 (240 mg, yield 34%) as a brown solid. LCMS: m/z calculated for C₁₂H₁₂Cl₂N₃OP: 316.12; found: 316.59 [M+H] ⁺.

Step 4: A mixture of compound 19-3 (160 mg, 0.506 mmol) , K₂CO₃ (350 mg, 2.53 mmol) and compound 1-11 (160 mg, 0.658 mmol) in DMAc (4 mL) was stirred at 120 ℃ for 16 h. After completion of the reaction, the mixture was diluted with EtOAc and filtered. The filtrate was concentrated under high vacuum. The residue was purified by pre-HPLC (C18 column, eluted with acetonitrile in water, TFA condition) . The desired components were lyophilized to afford the title compound 19 (55 mg, yield 24%) as a yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 7.97 (ddd, J = 11.9, 7.4, 1.9 Hz, 1H) , 7.59 (td, J = 7.5, 1.5 Hz, 1H) , 7.57 –7.51 (m, 2H) , 4.25 –4.04 (m, 3H) , 3.88 (d, J = 9.1 Hz, 1H) , 3.70 (d, J = 9.1 Hz, 1H) , 3.38 (d, J = 4.8 Hz, 1H) , 3.06 –2.93 (m, 2H) , 1.83 (d, J = 13.6 Hz, 6H) , 1.75 –1.65 (m, 3H) , 1.55 (d, J = 13.3 Hz, 1H) , 1.21 (d, J = 6.6 Hz, 3H) . LCMS: m/z calculated for C₂₁H₂₉ClN₅O₂P: 449.92; found: 450.71 [M+H] ⁺.

Example 20.

Synthesis of (3- (5- ( (3S, 4S) -4-amino-3-methyl-2-oxa-8-azaspiro [4.5] decan-8-yl) pyrazin-2-yl) -2-chlorophenyl) dimethylphosphine oxide (20)

Synthetic scheme 20

Step 1: Under nitrogen atmosphere, a mixture of compound 19-2 (520 mg, 2.23 mmol) , compound 7-1 (560 mg, 2.89 mmol) , Pd (dppf) Cl₂ (162 mg, 0.223 mmol) and K₃PO₄ (1.42 g, 6.69 mmol) in dioxane (8 mL) and H₂O (0.8 mL) was stirred at 90 ℃ for 1 h. After completion of the reaction, the mixture was cooled to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 6%MeOH in DCM) to afford the title compound 20-1 (400 mg, yield 59%) as a wheat solid. LCMS: m/z calculated for C₁₂H₁₁Cl₂N₂OP: 301.11; found: 301.56 [M+H] ⁺.

Step 2: A mixture of compound 20-1 (90 mg, 0.299 mmol) , K₂CO₃ (210 mg, 1.52 mmol) and compound 1-11 (100 mg, 0.411 mmol) in DMAc (3 mL) was stirred at 120 ℃ for 16 h. After completion of the reaction, the mixture was diluted with EtOAc and filtered. The filtrate was concentrated under high vacuum. The residue was purified by pre-HPLC (C18 column, eluted with MeCN in water, TFA condition) . The desired components were lyophilized to afford the title compound 20 (60 mg, yield 46%) as a yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ8.46 (d, J = 1.5 Hz, 1H) , 8.35 (d, J = 1.4 Hz, 1H) , 7.98 (ddd, J = 11.9, 7.5, 1.8 Hz, 1H) , 7.72 (dd, J = 7.8, 1.7 Hz, 1H) , 7.62 (td, J = 7.6, 1.5 Hz, 1H) , 4.32 –4.17 (m, 3H) , 3.91 (d, J = 9.1 Hz, 1H) , 3.72 (d, J = 9.1 Hz, 1H) , 3.40 (d, J = 4.8 Hz, 1H) , 3.18 –3.07 (m, 2H) , 1.87 (s, 3H) , 1.83 (s, 3H) , 1.74 (td, J = 13.1, 12.0, 4.1 Hz, 3H) , 1.59 (d, J = 13.4 Hz, 1H) , 1.22 (d, J = 6.5 Hz, 3H) . LCMS: m/z calculated for C₂₁H₂₈ClN₄O₂P: 434.90; found: 435.89 [M+H] ⁺.

Example 21.

Synthesis of (3- ( (5- ( (3S, 4S) -4-amino-3-methyl-2-oxa-8-azaspiro [4.5] decan-8-yl) -6- (hydroxymethyl) -3-methylpyrazin-2-yl) thio) -2-chlorophenyl) dimethylphosphine oxide (21)

Synthetic scheme 21

Step 1: To a clear solution of triphenylphosphine (6.0 g, 22.9 mmol) in anhydrous dioxane (50 mL) was added N-Chlorosuccinimide (3.05 g, 22.8 mmol) at room temperature. After stirring for 30 min, the mixture became a thick and white slurry. To this was added compound 21-1 (2.0 g, 7.66 mmol) in one portion. Then the mixture was heated to 100 ℃ and stirred for 1 h. After completion of the reaction, the mixture was cooled to room temperature and treated with TEA (20 mL) . The resulted mixture was extracted with EtOAc (100 mL x 2) and H₂O (100 mL) . The organic layers were washed with brine, dried over anhydrous Na₂SO₄, filtered and concentrated. The residue was purified by silica gel column (eluted with 10%EtOAc in petroleum ether) to afford the title compound 21-2 (1.9 g, yield 88%) as an orange solid.

Step 2: To a mixture of compound 21-2 (400 mg, 1.43 mmol) in DMAc (5 mL) was added compound 1-11 (420 mg, 1.72 mmol) and DIPEA (925 mg, 7.15 mmol) at room temperature. The mixture was stirred at 60 ℃ for 16 h. After completion of the reaction, the mixture was cooled and diluted with EtOAc (50 mL) . The organic layer was washed with saturated NH₄Cl solution and brine. The NH₄Cl solution was extracted again with EtOAc (50 mL) and the combined organic layers were dried over anhydrous Na₂SO₄, filtered and concentrated. The residue was purified by silica gel column (eluted with 10%MeOH in dichloromethane) to afford the title compound 21-3 (263 mg, yield 44%) as a yellow solid. LCMS: m/z calculated for C₁₇H₂₅BrN₄O₃: 413.32; found: 413.73 [M+H] ⁺.

Step 3: Under nitrogen atmosphere, to a solution of compound 21-3 (263 mg, 0.636 mmol) in anhydrous dichloromethane (5 mL) was added dropwise DIBAL-H (1 M, 2.54 mL, 2.54 mmol) at -78 ℃. After stirring at -78 ℃ for 30 min, the mixture was warmed slowly to room temperature and stirred for further 3 h. After completion of the reaction, the mixture was cooled to 0 ℃ in an ice bath and quenched by adding slowly 15%NaOH aqueous solution (1 mL) . The resulted mixture was stirred at room temperature for 30 min and extracted with dichloromethane (30 mL x 2) . The organic layers were washed with brine, dried over anhydrous Na₂SO₄, filtered and concentrated. The residue was purified by reverse phase flash chromatography (C18 column, eluted with acetonitrile in water, TFA condition) . The desired components were lyophilized to afford the title compound 21-4 (150 mg, yield 63%) as a yellow solid. LCMS: m/z calculated for C₁₅H₂₃BrN₄O₂: 371.28; found: 371.76 [M+H] ⁺.

Step 4: Under nitrogen atmosphere, a mixture of compound 21-4 (130 mg, 0.350 mmol) , compound 8-2 (80 w%, 110 mg, 0.362 mmol) , Pd₂ (dba) ₃ (32 mg, 0.35 mmol) , XantPhos (40 mg, 0.069 mmol) and DIPEA (135 mg, 1.04 mmol) in dioxane (3 mL) was stirred at 105 ℃for 16 h. After completion of the reaction, the mixture was cooled to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 15%MeOH in DCM) to afford the crude product, which was purified by reverse phase flash chromatography (C18 column, eluted with acetonitrile in water, TFA condition) . The desired components were lyophilized to afford the title compound 21 (38 mg, yield 21%) as a pale-yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 7.76 (ddd, J = 11.7, 7.6, 1.6 Hz, 1H) , 7.40 (td, J = 7.8, 1.7 Hz, 1H) , 7.06 (dd, J = 7.9, 1.5 Hz, 1H) , 4.42 (s, 2H) , 4.23 –4.16 (m, 1H) , 3.85 (d, J = 9.0 Hz, 1H) , 3.83 –3.71 (m, 2H) , 3.68 (d, J = 9.1 Hz, 1H) , 3.41 (d, J = 4.7 Hz, 1H) , 3.01 (qd, J = 13.6, 2.9 Hz, 2H) , 2.42 (s, 3H) , 1.82 (d, J = 13.6 Hz, 8H) , 1.74 (d, J = 13.3 Hz, 1H) , 1.60 (d, J = 13.1 Hz, 1H) , 1.20 (d, J = 6.6 Hz, 3H) . LCMS: m/z calculated for C₂₃H₃₂ClN₄O₃PS: 511.02; found: 511.91 [M+H] ⁺.

Example 22.

Synthesis of (3- ( (5- ( (3S, 4S) -4-amino-3-methyl-2-oxa-8-azaspiro [4.5] decan-8-yl) -6- (hydroxymethyl) pyrazin-2-yl) thio) -2-chlorophenyl) dimethylphosphine oxide (22)

Synthetic scheme 22

Step 1: To a solution of compound 22-1 (5.0 g, 32.4 mmol) in DMF (30 mL) was added N-Bromosuccinimide (5.78 g, 32.5 mmol) . The mixture was stirred at room temperature for 12 h. After completion of the reaction, the mixture was poured into H₂O (300 mL) an extracted with EtOAc (200 mL x 2) . The organic layers were washed with brine, dried over anhydrous, filtered and concentrated. The residue was purified by silica gel column (eluted with 15%EtOAc in petroleum ether) to afford the title compound 22-2 (3.4 g, yield 45%) as a pale-yellow solid. LCMS: m/z calculated for C₆H₅BrN₂O₃: 233.02; found: 233.57 [M+H] ⁺.

Step 2: Under nitrogen atmosphere, to a clear solution of triphenylphosphine (9.44 g, 36.0 mmol) in anhydrous dioxane (70 mL) was added N-Chlorosuccinimide (4.8 g, 35.9 mmol) at room temperature. After stirring for 30 min, the mixture became a thick and white slurry. To this was added compound 21-1 (2.8 g, 12.0 mmol) in one portion. Then the mixture was heated to 100 ℃ and stirred for 2 h. After completion of the reaction, the mixture was cooled to room temperature and poured into water (300mL) and extracted by dichloromethane (150 mL x 2) . The organic layers were washed with brine, dried over anhydrous Na₂SO₄, filtered and concentrated. The residue was purified by silica gel column (eluted with 10%EtOAc in petroleum ether) to afford the title compound 22-3 (2.1 g, yield 70%) as an off-white solid.

Step 3: To a mixture of compound 22-3 (400 mg, 1.59 mmol) in DMAc (5 mL) was added compound 1-11 (500 mg, 2.05 mmol) and DIPEA (1.03 g, 7.97 mmol) at room temperature. The mixture was stirred at 55 ℃ for 16 h. After completion of the reaction, the mixture was cooled and diluted with EtOAc (50 mL) . The organic layer was washed with saturated NH₄Cl solution and brine. The NH₄Cl solution was extracted again with EtOAc (50 mL) and the combined organic layers were dried over anhydrous Na₂SO₄, filtered and concentrated. The residue was purified by silica gel column (eluted with 10%MeOH in dichloromethane) to afford the title compound 22-4 (320 mg, yield 52%) as a yellow solid. LCMS: m/z calculated for C₁₅H₂₁BrN₄O₃: 385.26; found: 385.74 [M+H] ⁺.

Step 4: Under nitrogen atmosphere, to a solution of compound 22-4 (300 mg, 0.778 mmol) in anhydrous THF (5 mL) was added dropwise DIBAL-H (1 M, 3.12 mL, 3.12 mmol) at -75 ℃. After stirring at -75 ℃ for 30 min, the mixture was warmed slowly to room temperature and stirred for further 3 h. After completion of the reaction, the mixture was cooled to 0 ℃ in an ice bath and quenched by adding slowly 15%NaOH aqueous solution (1 mL) . The resulted mixture was stirred at room temperature for 30 min and extracted with dichloromethane (30 mL x 2) . The organic layers were washed with brine, dried over anhydrous Na₂SO₄, filtered and concentrated. The residue was purified by reverse phase flash chromatography (C18 column, eluted with acetonitrile in water, TFA condition) . The desired components were lyophilized to afford the title compound 22-5 (85 mg, yield 30%) as a yellow solid. LCMS: m/z calculated for C₁₄H₂₁BrN₄O₂: 357.25; found 357.68 [M+H] ⁺.

Step 5: Under nitrogen atmosphere, a mixture of compound 22-5 (70 mg, 0.196 mmol) , compound 8-2 (80 w%, 60 mg, 0.199 mmol) , Pd₂ (dba) ₃ (18 mg, 0.019 mmol) , XantPhos (23 mg, 0.039 mmol) and DIPEA (80 mg, 0.619 mmol) in dioxane (2 mL) was stirred at 105 ℃for 16 h. After completion of the reaction, the mixture was cooled to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 15%MeOH in DCM) to afford the crude product, which was purified by reverse phase flash chromatography (C18 column, eluted with acetonitrile in water, TFA condition) . The desired components were lyophilized to afford the title compound 22 (22 mg, yield 22%) as an off-white solid. ¹H NMR (400 MHz, DMSO-d₆) δ 8.21 (s, 1H) , 7.82 –7.76 (m, 1H) , 7.43 (td, J = 7.8, 1.7 Hz, 1H) , 7.26 (dd, J = 8.0, 1.6 Hz, 1H) , 4.49 (s, 2H) , 4.24 –4.17 (m, 1H) , 3.88 –3.67 (m, 4H) , 3.40 (d, J = 4.7 Hz, 1H) , 3.08 –2.95 (m, 2H) , 1.83 (d, J = 13.6 Hz, 8H) , 1.74 (d, J = 13.3 Hz, 1H) , 1.59 (d, J = 12.9 Hz, 1H) , 1.21 (d, J = 6.6 Hz, 3H) . LCMS: m/z calculated for C₂₂H₃₀ClN₄O₃PS: 496.99; found: 497.84 [M+H] ⁺.

Example 23.

Synthesis of (3- (3-amino-5- (4-amino-4-methylpiperidin-1-yl) pyrazin-2-yl) -2-chlorophenyl) dimethylphosphine oxide (23)

Synthetic scheme 23

Step 1: A mixture of compound 19-3 (110 mg, 0.348 mmol) , K₂CO₃ (120 mg, 0.868 mmol) and compound 16-5 (110 mg, 0.514 mmol) in DMAc (4 mL) and H₂O (0.4 mL) was stirred at 120 ℃ for 3 h. After completion of the reaction, the mixture was extracted with EtOAc (3 x 30 mL) and H₂O (30 mL) . The combined organic layers were washed with brine, dried over anhydrous Na₂SO₄ and concentrated under reduced pressure. The residue was purified by silica gel column (eluted with 5%MeOH in dichloromethane) to afford the title compound 23-1 (60 mg, yield 35%) as a pale-yellow solid. LCMS: m/z calculated for C₂₃H₃₃ClN₅O₃P: 493.97; found: 493.20 [M+H] ⁺.

Step 2: To a solution of compound 23-1 (60 mg, 0.293 mmol) in dichloromethane (2 mL) was added TFA (1 mL) . Then the mixture was stirred at room temperature for 1 h. After completion of the reaction, the mixture was concentrated under vacuum. The residue was purified by pre-HPLC (C18 column, eluted with MeCN in water, TFA condition) . The desired components were lyophilized to afford the title compound 23 (23 mg, yield 20%) as a yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 7.97 (ddd, J = 11.9, 7.5, 1.9 Hz, 1H) , 7.62 –7.50 (m, 3H) , 4.01 (dt, J = 13.9, 4.5 Hz, 2H) , 3.24 (ddd, J = 13.7, 8.4, 5.4 Hz, 2H) , 1.82 (d, J = 13.6 Hz, 6H) , 1.72 (p, J = 5.6, 4.7 Hz, 4H) , 1.37 (s, 3H) . LCMS: m/z calculated for C₁₈H₂₅ClN₅OP: 393.86; found: 394.66 [M+H] ⁺.

Example 24.

Synthesis of (S) - (3- (3-amino-5- (1-amino-1, 3-dihydrospiro [indene-2, 4'-piperidin] -1'-yl) pyrazin-2-yl) -2-chlorophenyl) dimethylphosphine oxide (24)

Synthetic scheme 24

Step 1: A mixture of compound 19-3 (110 mg, 0.348 mmol) , K₂CO₃ (240 mg, 1.73 mmol) and compound 18-1 (125 mg, 0.453 mmol) in DMAc (3 mL) was stirred at 100 ℃ for 16 h. After completion of the reaction, the mixture was cooled, diluted with EtOAc and concentrated. The residue was purified by pre-HPLC (C18 column, eluted with acetonitrile in water, TFA condition) . The desired components were lyophilized to afford the title compound 24 (25 mg, yield 14%) as a yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 7.97 (ddd, J = 11.9, 7.4, 2.0 Hz, 1H) , 7.61 –7.49 (m, 4H) , 7.39 –7.28 (m, 3H) , 4.37 (s, 1H) , 4.20 (dd, J = 35.3, 13.8 Hz, 2H) , 3.18 –3.00 (m, 4H) , 1.83 (d, J = 13.6 Hz, 6H) , 1.76 –1.66 (m, 2H) , 1.57 –1.45 (m, 2H) . LCMS: m/z calculated for C₂₅H₂₉ClN₅OP: 481.96; found: 482.89 [M+H] ⁺.

Example 25.

Synthesis of (3- (5- (4-amino-4-methylpiperidin-1-yl) pyrazin-2-yl) -2-chlorophenyl) dimethylphosphine oxide (25)

Synthetic scheme 25

Step 1: A mixture of compound 20-1 (70 mg, 0.232 mmol) , K₂CO₃ (80 mg, 0.579 mmol) and compound 16-5 (74 mg, 0.346 mmol) in DMAc (3 mL) and H₂O (0.3 mL) was stirred at 120 ℃ for 3 h. After completion of the reaction, the mixture was extracted with EtOAc (3 x 20 mL) and H₂O (20 mL) . The combined organic layers were washed with brine, dried over anhydrous Na₂SO₄ and concentrated under reduced pressure. The residue was purified by silica gel column (eluted with 5%MeOH in dichloromethane) to afford the title compound 25-1 (40 mg, yield 36%) as a pale-yellow solid. LCMS: m/z calculated for C₂₃H₃₂ClN₄O₃P: 478.96; found: 479.87 [M+H] ⁺.

Step 2: To a solution of compound 25-1 (40 mg, 0.0835 mmol) in dichloromethane (2 mL) was added TFA (1 mL) . Then the mixture was stirred at room temperature for 1 h. After completion of the reaction, the mixture was concentrated under vacuum. The residue was purified by pre-HPLC (C18 column, eluted with acetonitrile in water, TFA condition) . The desired components were lyophilized to afford the title compound 25 (25 mg, yield 20%) as a yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 8.47 (d, J = 1.5 Hz, 1H) , 8.36 (d, J = 1.4 Hz, 1H) , 7.99 (ddd, J = 11.9, 7.6, 1.8 Hz, 1H) , 7.71 (dd, J = 7.6, 1.8 Hz, 1H) , 7.62 (td, J = 7.6, 1.6 Hz, 1H) , 4.14 (dt, J = 13.9, 4.4 Hz, 2H) , 3.36 (dt, J = 13.8, 6.9 Hz, 2H) , 1.84 (d, J = 13.6 Hz, 6H) , 1.76 (t, J = 5.9 Hz, 4H) , 1.39 (s, 3H) . LCMS: m/z calculated for C₁₈H₂₄ClN₄OP: 378.84; found: 379.77 [M+H] ⁺.

Example 26.

Synthesis of (S) - (3- (5- (1-amino-1, 3-dihydrospiro [indene-2, 4'-piperidin] -1'-yl) pyrazin-2-yl) -2-chlorophenyl) dimethylphosphine oxide (26)

Synthetic scheme 26

Step 1: A mixture of compound 20-1 (80 mg, 0.265 mmol) , K₂CO₃ (185 mg, 1.34 mmol) and compound 18-1 (95 mg, 0.344 mmol) in DMAc (2 mL) was stirred at 100 ℃ for 16 h. After completion of the reaction, the mixture was cooled, diluted with EtOAc and concentrated. The residue was purified by pre-HPLC (C18 column, eluted with MeCN in water, TFA condition) . The desired components were lyophilized to afford the title compound 26 (48 mg, yield 38%) as a yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 8.47 (d, J = 1.5 Hz, 1H) , 8.36 (d, J = 1.4 Hz, 1H) , 7.98 (ddd, J = 11.9, 7.5, 1.8 Hz, 1H) , 7.73 (dd, J = 7.7, 1.8 Hz, 1H) , 7.62 (td, J = 7.6, 1.5 Hz, 1H) , 7.50 (d, J = 7.4 Hz, 1H) , 7.40 –7.29 (m, 3H) , 4.39 (s, 2H) , 4.28 (d, J = 13.7 Hz, 1H) , 3.30 –3.15 (m, 3H) , 3.05 (d, J = 16.2 Hz, 1H) , 1.84 (d, J = 13.6 Hz, 6H) , 1.81 –1.70 (m, 2H) , 1.55 (t, J = 13.2 Hz, 2H) . LCMS: m/z calculated for C₂₅H₂₈ClN₄OP: 466.95; found: 467.79 [M+H] ⁺.

Example 27.

Synthesis of (3- (5- ( (3S, 4S) -4-amino-3-methyl-2-oxa-8-azaspiro [4.5] decan-8-yl) -6- (hydroxymethyl) -3-methylpyrazin-2-yl) -2-chlorophenyl) dimethylphosphine oxide (27)

Synthetic scheme 27

Step 1: Under nitrogen atmosphere, a mixture of compound 21-4 (120 mg, 0.323 mmol) , compound 19-2 (90 mg, 0.387 mmol) , Pd (dppf) Cl₂ (25 mg, 0.34 mmol) and K₃PO₄ (205 mg, 0.967 mmol) in dioxane (3 mL) and H₂O (0.3 mL) was stirred at 90 ℃ for 1 h. After completion of the reaction, the mixture was cooled to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 10%MeOH in DCM) to afford the crude product, which was purified by reverse phase flash chromatography (C18 column, eluted with acetonitrile in water, TFA condition) . The desired components were lyophilized to afford the title compound 27 (65 mg, yield 42%) as a yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 8.02 (ddd, J = 11.9, 6.9, 2.5 Hz, 1H) , 7.67 –7.58 (m, 2H) , 4.50 (s, 2H) , 4.25 –4.17 (m, 1H) , 3.86 (d, J = 9.0 Hz, 1H) , 3.69 (q, J = 12.1, 10.2 Hz, 3H) , 3.55 (s, 3H) , 3.43 (d, J = 4.6 Hz, 1H) , 3.04 –2.91 (m, 2H) , 1.93 –1.74 (m, 9H) , 1.63 (d, J = 12.9 Hz, 1H) , 1.21 (d, J = 6.5 Hz, 3H) . LCMS: m/z calculated for C₂₃H₃₂ClN₄O₃P: 478.96; found: 479.87 [M+H] ⁺.

Example 28.

Synthesis of methyl 3- ( (3S, 4S) -4-amino-3-methyl-2-oxa-8-azaspiro [4.5] decan-8-yl) -6- ( (2- chloro-3- (dimethylphosphoryl) phenyl) thio) pyrazine-2-carboxylate (28)

Synthetic scheme 28

Step 1: Under nitrogen atmosphere, a mixture of compound 22-4 (160 mg, 0.415 mmol) , compound 8-2 (80 w%, 130 mg, 0.428 mmol) , Pd₂ (dba) ₃ (38 mg, 0.041 mmol) , XantPhos (48 mg, 0.083 mmol) and DIPEA (160 mg, 1.24 mmol) in dioxane (3 mL) was stirred at 105 ℃ for 16 h. After that, the mixture was cooled to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 20%MeOH in DCM) to afford the crude product, which was purified by reverse phase flash chromatography (C18 column, eluted with acetonitrile in water, TFA condition) . The desired components were lyophilized to afford the title compound 28 (5.7 mg, yield 2%) as an off-white solid.. ¹H NMR (400 MHz, DMSO-d₆) δ 8.40 (s, 1H) , 7.79 (ddd, J = 11.7, 7.6, 1.6 Hz, 1H) , 7.43 (td, J = 7.8, 1.7 Hz, 1H) , 7.20 –7.16 (m, 1H) , 4.24 –4.16 (m, 1H) , 3.87 –3.81 (m, 5H) , 3.76 –3.67 (m, 2H) , 3.42 (d, J = 4.7 Hz, 1H) , 3.21 –3.08 (m, 2H) , 1.84 (s, 3H) , 1.80 (s, 3H) , 1.79 –1.71 (m, 3H) , 1.57 (d, J = 13.4 Hz, 1H) , 1.20 (d, J = 6.5 Hz, 3H) . LCMS: m/z calculated for C₂₃H₃₀ClN₄O₄PS: 525.00; found: 525.83 [M+H] ⁺.

Example 29.

Synthesis of (3- ( (5- ( (3S, 4S) -4-amino-3-methyl-2-oxa-8-azaspiro [4.5] decan-8-yl) -3- methylpyrazin-2-yl) thio) -2-chlorophenyl) dimethylphosphine oxide (29)

Synthetic scheme 29

Step 1: To a solution of compound 29-1 (1.0 g, 9.08 mmol) in DMSO (20 mL) and H₂O (2 mL) at room temperature was added NBS (800 mg, 4.49 mmol) slowly. The mixture was stirred at room temperature for 16 h. After completion of the reaction, the mixture diluted with EtOAc (50 mL) and washed with water. The organic layer was washed with brine, dried over anhydrous Na₂SO₄, filtered and concentrated. The residue was purified by reverse phase flash (C18 column, eluted with acetonitrile in H₂O, TFA condition) . The desired components were lyophilized to afford the title compound 29-2 (566 mg, yield 33%) as a pale-brown solid. LCMS: m/z calculated for C₅H₅BrN₂O: 189.01; found: 189.46 [M+H] ⁺.

Step 2: Under nitrogen atmosphere, a mixture of compound 29-2 (380 mg, 2.01 mmol) , compound 8-2 (80 w%, 610 mg, 2.01 mmol) , Pd₂ (Dba) ₃ (185 mg, 0.202 mmol) , Xantphos (232 mg, 0.400) and DIPEA (780 mg, 6.03 mmol) in dioxane (5 mL) was stirred at 115 ℃ for 16 h. Then the mixture was cooled to room temperature and concentrated. The residue was purified by silica gel column (eluted with 15%MeOH in dichloromethane) to give a crude product, which was purified furtherly by reverse phase flash (C18 column, eluted with acetonitrile in H₂O, TFA condition) . The desired components were lyophilized to afford the title compound 29-3 (56 mg, yield 8%) as a pale-yellow solid. LCMS: m/z calculated for C₁₃H₁₄ClN₂O₂PS: 328.75; found: 329.69 [M+H] ⁺.

Step 3: A mixture of compound 29-3 (56 mg, 0.170 mmol) , DBU (52 mg, 0.341 mmol) , compound 1-11 (42 mg, 0.172 mmol) and BOP (75 mg, 0.170 mmol) in DMF (2 mL) was stirred at room temperature for 16 h. After completion of the reaction, the mixture was quenched by adding H₂O (1 mL) . The resulted mixture was filtered and purified directly by pre-HPLC (C18 column, eluted with acetonitrile in water, TFA condition) . The desired components were lyophilized to afford the title compound 29 (14 mg, yield 17%) as a yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 8.26 (s, 1H) , 7.73 (ddd, J = 11.7, 7.6, 1.6 Hz, 1H) , 7.38 (td, J = 7.7, 1.7 Hz, 1H) , 6.95 (dd, J = 8.1, 1.7 Hz, 1H) , 4.29 –4.14 (m, 3H) , 3.88 (d, J = 9.1 Hz, 1H) , 3.70 (d, J = 9.1 Hz, 1H) , 3.39 (d, J = 4.8 Hz, 1H) , 3.18 –3.05 (m, 2H) , 2.40 (s, 3H) , 1.82 (d, J = 13.6 Hz, 6H) , 1.76 –1.64 (m, 3H) , 1.57 (d, J = 13.4 Hz, 1H) , 1.20 (d, J = 6.5 Hz, 3H) . LCMS: m/z calculated for C₂₂H₃₀ClN₄O₂PS: 480.99; found: 481.79 [M+H] ⁺.

Example 30.

Synthesis of (3- ( (3-amino-5- (4-amino-4-methylpiperidin-1-yl) pyrazin-2-yl) thio) -2-chlorophenyl) dicyclopropylphosphine oxide (30)

Synthetic scheme 30

Step 1: Under nitrogen atmosphere, a solution of compound 16-1 (5 g, 36.2 mmol) in anhydrous THF (50 mL) was added dropwise to a flask containing cyclopropylmagnesium bromide (0.5 M, 217 mL, 108.6 mmol) at -20 ℃. The mixture was maintained at -20 ℃ for 15 min after addition, then allowed to warm slowly to room temperature and stirred for a further 20 h. After completion of the reaction, the mixture was quenched with 1 N HCl. The resulting mixture was extracted with MTBE and EtOAc. The organic layers were combined, dried over anhydrous Na₂SO₄, filtered and concentrated to afford the title compound 30-1 (3.1 g, crude, yield 65%) as an orange oil. The crude product was used directly in the next step without further purification.

Step 2: A mixture of compound 16-6 (230 mg, 0.4 mmol) , compound 30-1 (156 mg, 1.2 mmol) , Pd₂ (dba) ₃ (37 mg, 0.04 mmol) , Xantphos (46 mg, 0.08 mmol) and DIPEA (155 mg, 1.2 mmol) in dioxane (4 mL) was stirred at 105 ℃ for 16 h. After completion of the reaction, the mixture was cooled to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 5%MeOH in DCM) to afford the crude product, which was purified by reverse phase flash chromatography (C18 column, eluted with acetonitrile in water, TFA condition) . The desired components were lyophilized to afford the title compound 30-2 (30 mg, yield 15%) as a yellow solid. LCMS: m/z calculated for C₂₇H₃₇ClN₅O₃PS: 578.11; found: 578.93 [M+H] ⁺.

Step 3: A mixture of compound 30-2 (30 mg, 0.05 mmol) in DCM (1 mL) and TFA (0.3 mL) was stirred at room temperature for 1 h. After completion of the reaction, the mixture was concentrated under vacuum. The residue was purified by reverse phase flash chromatography (C18 column, eluted with acetonitrile in water, TFA condition) . The desired components were lyophilized to afford the title compound CAN2352 (11 mg, yield 44%) as a yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 7.68 (s, 1H) , 7.58 (ddd, J = 11.1, 7.5, 1.6 Hz, 1H) , 7.34 (td, J = 7.8, 1.7 Hz, 1H) , 6.77 (dd, J = 8.0, 1.6 Hz, 1H) , 4.10 –3.99 (m, 2H) , 3.29 (dt, J = 13.8, 6.9 Hz, 2H) , 1.71 (t, J = 5.7 Hz, 4H) , 1.56 (dtt, J = 14.8, 8.9, 5.9 Hz, 2H) , 1.37 (s, 3H) , 0.89 (ddtt, J = 14.8, 8.8, 5.9, 2.5 Hz, 4H) , 0.73 (qdd, J = 9.1, 5.9, 3.7 Hz, 2H) , 0.55 (dqd, J = 15.5, 6.0, 3.1 Hz, 2H) . LCMS: m/z calculated for C₂₂H₂₉ClN₅OPS: 477.99; found: 478.84 [M+H] ⁺.

Example 31.

Synthesis of (3- ( (3-amino-5- ( (3S, 4S) -4-amino-3-methyl-2-oxa-8-azaspiro [4.5] decan-8-yl) pyrazin-2-yl) thio) -2-chlorophenyl) dicyclopropylphosphine oxide (31)

Synthetic scheme 31

Step 1: A solution of compound 31-1 (1 g, 2.7 mmol) and TFA (3 mL) in anhydrous DCM (50 mL) was stirred at room temperature for 1h. After completion of the reaction, the mixture was concentrated under vacuum to afford the title compound 31-2 (1.9 g, crude trifluoroacetate, 54 w%) as a yellow oil. The crude product was used directly in the next step without further purification.

Step 2: A mixture of compound 31-2 (585 mg, 54 w%, 0.81 mmol) , compound 16-4 (250 mg, 0.71 mmol) and K₂CO₃ (483 mg, 3.5 mmol) in anhydrous DMAc (10 mL) was stirred at 100 ℃ for 2 h. After completion of the reaction, the mixture was cooled down to room temperature. The resulting mixture was extracted with EtOAc (50 mL x 3) and water. The combined organic layers were washed with brine_, dried over anhydrous Na₂SO₄ and concentrated under reduced pressure. The residue was purified by silica gel column (eluted with 3%MeOH in DCM) to afford the title compound 31-3 (360 mg, yield 86%) as a brown oil. LCMS: m/z calculated for C₂₃H₃₁BrClN₅O₂S₂: 589.01; found: 590.66 [M+H] ⁺.

Step 3: Under nitrogen atmosphere, a mixture of compound 31-3 (300 mg, 0.5 mmol) , compound 30-1 (195 mg, 1.5 mmol) , Pd₂ (dba) ₃ (46 mg, 0.05 mmol) , Xantphos (58 mg, 0.1 mmol) and DIPEA (194 mg, 1.5 mmol) in dioxane (6 mL) was stirred at 105 ℃ for 16 h. After completion of the reaction, the mixture was cooled to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 5%MeOH in DCM) to afford the crude product, which was purified by reverse phase flash chromatography (C18 column, eluted with acetonitrile in water, TFA condition) . The desired components were lyophilized to afford the title compound 31-4 (160 mg, yield 49%) as a yellow solid. LCMS: m/z calculated for C₂₉H₄₁ClN₅O₃PS₂: 638.22; found: 638.82 [M+H] ⁺.

Step 4: To a solution of compound 31-4 (160 mg, 0.25 mmol) and MeOH (2 mL) was added 4 N HCl in dioxane (0.2 mL, 0.9 mmol) was stirred at 40 ℃ for 2 h. After completion of the reaction, the mixture was concentrated under vacuum. The residue was purified by pre-HPLC (C18 column, eluted with acetonitrile in water, TFA condition) . The desired components were lyophilized to afford the title compound CAN2352 (27.8 mg, yield 20%) as a light-yellow solid. LCMS: m/z calculated for C₂₅H₃₃ClN₅O₂PS: 534.06; found: 534.82 [M+H] ⁺. ¹H NMR (400 MHz, DMSO-d6) δ 7.67 (s, 1H) , 7.58 (ddd, J = 11.2, 7.5, 1.6 Hz, 1H) , 7.34 (td, J = 7.8, 1.7 Hz, 1H) , 6.77 (dd, J = 7.9, 1.6 Hz, 1H) , 4.24 –4.10 (m, 3H) , 3.88 (d, J = 9.1 Hz, 1H) , 3.69 (d, J = 9.1 Hz, 1H) , 3.39 (d, J = 4.8 Hz, 1H) , 3.11 –2.99 (m, 2H) , 1.75 –1.64 (m, 3H) , 1.56 (dtt, J = 14.8, 8.9, 5.9 Hz, 3H) , 1.21 (d, J = 6.5 Hz, 3H) , 0.89 (ddtd, J = 17.3, 8.9, 6.1, 3.0 Hz, 4H) , 0.73 (qdd, J = 9.0, 6.0, 3.8 Hz, 2H) , 0.55 (dqd, J = 15.4, 6.0, 3.2 Hz, 2H) .

Example 32.

Synthesis of (S) - (3- ( (5- (1-amino-1, 3-dihydrospiro [indene-2, 4'-piperidin] -1'-yl) -6- (hydroxymethyl) pyrazin-2-yl) thio) -2-chlorophenyl) dimethylphosphine oxide (32)

Synthetic scheme 32

Step 1: To a mixture of compound 22-3 (1.1 g, 4.38 mmol) in DMAc (15 mL) was added compound 18-1 (1.2 g, 4.38 mmol) and DIPEA (2.83 g, 21.9 mmol) at room temperature. The mixture was stirred at 55 ℃ for 16 h. After completion of the reaction, the mixture was concentrated under vacuum. The residue was purified by reverse phase flash chromatography (C18 column, eluted with acetonitrile in water, HCl condition) . The desired components were lyophilized to afford the title compound 32-1 (1.4 g, yield 77%) as a yellow solid. LCMS: m/z calculated for C₁₉H₂₁BrN₄O₂: 417.31; found: 417.64 [M+H] ⁺.

Step 2: Under nitrogen atmosphere, to a solution of compound 32-1 (700 mg, 1.68 mmol) in anhydrous DCM (7 mL) was added dropwise DIBAL-H (1 M, 6.72 mL, 6.72 mmol) at -75 ℃. After stirring at -75 ℃ for 30 min, the mixture was warmed slowly to room temperature and stirred for further 1 h. After completion of the reaction, the mixture was cooled to 0 ℃ in an ice bath and quenched by adding slowly 15%NaOH aqueous solution (1.5 mL) . The resulted mixture was stirred at room temperature for 30 min and extracted with dichloromethane (40 mL x 2) . The organic layers were washed with brine, dried over anhydrous Na₂SO₄, filtered and concentrated. The residue was purified by reverse phase flash chromatography (C18 column, eluted with acetonitrile in water, HCl condition) . The desired components were lyophilized to afford the title compound 32-2 (89 mg, yield 14%) as a yellow oil. LCMS: m/z calculated for C₁₈H₂₁BrN₄O: 389.30; found 389.93 [M+H] ⁺.

Step 3: Under nitrogen atmosphere, a mixture of compound 32-2 (70 mg, 0.132 mmol) , compound 8-2 (60 w%, 160 mg, 0.396 mmol) , Pd₂ (dba) ₃ (12 mg, 0.013 mmol) , Xantphos (15 mg, 0.026 mmol) and DIPEA (68 mg, 0.528 mmol) in dioxane (2 mL) was stirred at 105 ℃for 16 h. After completion of the reaction, the mixture was cooled to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 15%MeOH in DCM) to afford the crude product, which was purified furtherly by pre-HPLC (C18 column, eluted with acetonitrile in water, TFA condition) . The desired components were lyophilized to afford the title compound 32 (9.3 mg, yield 10%) as a light-yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ8.17 (s, 1H) , 7.75 (ddd, J = 11.9, 7.6, 1.6 Hz, 1H) , 7.49–7.39 (m, 2H) , 7.34 (d, J = 5.5 Hz, 2H) , 7.28 (t, J = 8.3 Hz, 2H) , 4.50 (s, 2H) , 4.33 (s, 1H) , 3.79 (d, J = 13.3 Hz, 1H) , 3.70 (d, J = 13.4 Hz, 1H) , 3.20–3.07 (m, 3H) , 2.99 (d, J = 16.4 Hz, 1H) , 1.82 (d, J = 13.6 Hz, 8H) , 1.57 (d, J = 12.7 Hz, 1H) , 1.49 (d, J = 13.0 Hz, 1H) . LCMS: m/z calculated for C₂₆H₃₀ClN₄O₂PS: 529.04; found: 529.81 [M+H] ⁺.

Example 33.

Synthesis of 8- (5- ( (2-chloro-3- (dimethylphosphoryl) phenyl) thio) pyrazin-2-yl) -2, 8-diazaspiro [4.5] decan-3-one (33)

Synthetic scheme 33

Step 1: A mixture of 7-7 (60 mg, 0.18 mmol) , K₂CO₃ (124 mg, 0.90 mmol) and 33-1 (34 mg, 0.18 mmol) in DMAc (1.5 mL) was stirred at 100 ℃ for 3 h. After completion of the reaction, the mixture was filtered. The filtrate was concentrated and purified directly by pre-HPLC (C18 column, eluted with 47%MeCN in water, TFA condition) . The desired components were lyophilized to afford the title compound 33 (16 mg, yield 19%) as a yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 8.34 (s, 1H) , 8.20 (s, 1H) , 7.66 (dd, J = 12.7, 6.8 Hz, 1H) , 7.37 (t, J = 6.9 Hz, 1H) , 7.04 (d, J = 8.0 Hz, 1H) , 3.71 (dt, J = 13.5, 5.2 Hz, 2H) , 3.53 (dt, J = 13.1, 5.7 Hz, 2H) , 3.12 (s, 2H) , 2.16 (s, 2H) , 1.83 (s, 3H) , 1.80 (s, 3H) , 1.60 (t, J = 5.7 Hz, 4H) . LCMS: m/z calculated for C₂₀H₂₄ClN₄O₂PS: 450.10 found: 451.74 [M+H] ⁺.

Example 34.

Synthesis of 1'- (5- ( (2-chloro-3- (dimethylphosphoryl) phenyl) thio) pyrazin-2-yl) spiro [indoline-3, 4'-piperidin] -2-one (34)

Synthetic scheme 34

Step 1: A mixture of 7-7 (60 mg, 0.18 mmol) , K₂CO₃ (124 mg, 0.90 mmol) and 34-1 (36 mg, 0.18 mmol) in DMAc (1.5 mL) was stirred at 100℃ for 3 h. After completion of the reaction, the mixture was filtered. The filtrate was concentrated and purified directly by pre-HPLC (C18 column, eluted with 55%MeCN in water, TFA condition) . The desired components were lyophilized to afford the title compound 34 (18 mg, yield 20%) as a yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 8.46 (s, 1H) , 8.29 (s, 1H) , 7.71 (dd, J = 11.8, 6.0 Hz, 1H) , 7.48 –7.38 (m, 2H) , 7.21 (t, J = 7.7 Hz, 1H) , 7.09 (d, J = 8.0 Hz, 1H) , 6.97 (t, J = 7.0 Hz, 1H) , 6.90 (d, J = 7.7 Hz, 1H) , 4.10 –3.94 (m, 4H) , 1.81 (dd, J = 15.4, 7.0 Hz, 10H) . LCMS: m/z calculated for C₂₄H₂₄ClN₄O₂PS: 498.10; found: 499.76 [M+H] ⁺.

Example 35.

Synthesis of (S) - (6-amino-3- ( (5- (1-amino-1, 3-dihydrospiro [indene-2, 4'-piperidin] -1'-yl) pyrazin-2-yl) thio) -2-chlorophenyl) dimethylphosphine oxide (35)

Synthetic scheme 35

Step 1: A mixture of compound 35-1 (4.0 g, 15.81 mmol) , compound 1-7 (6.2 g, 79.05 mmol) , Pd₂ (dba) ₃ (1.4 g, 1.58 mmol) , Xantphos (1.8 g, 3.16 mmol) and DIPEA (6.1 g, 47.43 mmol) in dioxane (100 mL) was stirred at 105 ℃ under nitrogen atmosphere for 16 h. After completion of the reaction, the mixture was cooled down to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 1.6%MeOH in DCM) to afford 4.3 g of crude product. The crude product was furtherly purified by reverse phase flash chromatography (C18 column, eluted with 69%MeCN in water, TFA condition) . The desired components were lyophilized to afford title compound 35-2 (2.4 g, yield 75%) as a yellow solid. LCMS: m/z calculated for C₈H₁₁ClNOP: 203.03; found: 204.42 [M+H] ⁺.

Step 2: To a solution of compound 35-2 (2.2 g, 10.78 mmol) in AcOH (40 mL) was added NIS (2.4 g, 10.78 mmol) . The reaction mixture was stirred at room temperature for 3 h. After completion of the reaction, the mixture was concentrated. The crude product was purified by reverse phase flash chromatography (C18 column, eluted with 54%MeCN in water, TFA condition) . The desired components were lyophilized to afford the title compound 35-3 (2.0 g, yield 56%) as a brown solid. ¹HNMR (400 MHz, DMSO-d6) : δ 7.60 (d, J = 8.8 Hz, 1H) , 6.43 (dd, J = 8.9, 3.8 Hz, 1H) , 1.88 (d, J = 13.6 Hz, 6H) . LCMS: m/z calculated for C₈H₁₀ClINOP: 328.92; found: 330.58 [M+H] ⁺.

Step 3: A mixture of compound 35-3 (450 mg, 1.36 mmol) , 1-2 (327 mg, 2.73 mmol) , Pd₂ (dba) ₃ (125 mg, 0.14 mmol) , Xantphos (158 mg, 0.27 mmol) and DIPEA (526 mg, 4.08 mmol) in dioxane (10 mL) was stirred at 105 ℃ under nitrogen atmosphere for 3 h. After completion of the reaction, the mixture was cooled down to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 2.5%MeOH in DCM) to afford title compound 35-4 (393 mg, yield 89%) as a brown solid. LCMS: m/z calculated for C₁₂H₁₇ClNO₃PS: 321.04; found: 322.62 [M+H] ⁺.

Step 4: Under nitrogen atmosphere, to a solution of compound of 35-4 (393 mg, 1.22 mmol) in THF (4.0 mL) was added dropwise EtONa (20%in EtOH, 830 mg, 2.44 mmol) at -30 ℃. The reaction mixture was stirred at -30 ℃ for 1 h. After completion of the reaction, the mixture was concentrated under vacuum. The crude product was dispersed with 40 mL of cold solvent (petroleum ether/DCM=1: 1) . The insoluble precipitate was dried under vacuum after filtration to afford the title compound 35-5 (590 mg, crude, 53 wt%) as a yellow solid. LCMS: m/z calculated for C₈H₁₀ClNNaOPS: 256.98; found: 236.39. [M-Na+2H] ⁺.

Step 5: A mixture of compound 35-5 (590 mg, 53 wt%, 1.22 mmol) , 7-1 (276.8 mg, 1.43 mmol) , Pd₂ (dba) ₃ (128 mg, 0.14 mmol) , Xantphos (166 mg, 0.27 mmol) and DIPEA (553 mg, 4.29 mmol) in dioxane (10 mL) was stirred at 105 ℃ under nitrogen atmosphere for 3 h. After completion of the reaction, the mixture was cooled down to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 3.3%MeOH in DCM) to afford title compound 35-6 (148 mg, yield 35%over 2 steps) as a brown solid. LCMS: m/z calculated for C₁₂H₁₂Cl₂N₃OPS: 346.98; found: 348.69 [M+H] ⁺.

Step 6: A mixture of compound 35-6 (60 mg, 0.17 mmol) , K₂CO₃ (147mg, 1.06 mmol) and compound 18-1 (59 mg, 0.21 mmol) in DMAc (2 mL) was stirred at 120 ℃ for 12 h. After completion of the reaction, the mixture was cooled and filtered. The filtrate was concentrated and purified by pre-HPLC (C18 column, eluted with 39%MeCN in water, TFA condition) to afford the title compound 35 (28 mg, yield 32%) as a yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 8.29 (d, J = 1.5 Hz, 1H) , 7.90 (d, J = 1.4 Hz, 1H) , 7.49 (d, J = 7.4 Hz, 1H) , 7.38 –7.23 (m, 4H) , 6.61 (dd, J = 8.8, 3.7 Hz, 1H) , 4.35 (s, 1H) , 4.18 (dd, J = 36.2, 13.8 Hz, 2H) , 3.21 –3.09 (m, 3H) , 2.99 (d, J = 16.3 Hz, 1H) , 1.88 (d, J = 13.6 Hz, 6H) , 1.76 –1.64 (m, 2H) , 1.49 (t, J = 12.1 Hz, 2H) . LCMS: m/z calculated for C₂₅H₂₉ClN₅OPS: 513.15; found: 497.64 [M-OH+H] ⁺.

Example 36.

Synthesis of (S) - (6-amino-3- ( (3-amino-5- (1-amino-1, 3-dihydrospiro [indene-2, 4'-piperidin] -1'-yl) pyrazin-2-yl) thio) -2-chlorophenyl) dimethylphosphine oxide (36)

Synthetic scheme 36

Step 1: A mixture of compound 35-5 (413 mg, 53 wt%, 0.85 mmol) , compound 1-1 (177 mg, 0.85 mmol) , Pd₂ (dba) ₃ (78 mg, 0.09 mmol) , Xantphos (98 mg, 0.17 mmol) and DIPEA (329 mg, 2.55 mmol) in dioxane (5 mL) was stirred at 105 ℃ under nitrogen atmosphere for 3 h. After completion of the reaction, the mixture was cooled down to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 5%MeOH in DCM) to afford title compound 36-1 (120 mg, yield 39%) as a brown solid. LCMS: m/z calculated for C₁₂H₁₃Cl₂N₄OPS: 361.99; found: 363.72 [M+H] ⁺.

Step 2: A mixture of compound 36-1 (60 mg, 0.17 mmol) , K₂CO₃ (114 mg, 0.83 mmol) and compound 18-1 (47 mg, 0.17 mmol) in DMAc (2 mL) was stirred at 120 ℃ for 12 h. After completion of the reaction, the mixture was filtered. The filtrate was concentrated and purified by pre-HPLC (C18 column, eluted with 27%MeCN in water, TFA condition) . The desired components were lyophilized to afford the title compound 36 (21 mg, yield 24%) as a yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 7.57 (s, 1H) , 7.51 (d, J = 7.4 Hz, 1H) , 7.39 –7.27 (m, 3H) , 6.80 (d, J = 8.8 Hz, 1H) , 6.55 (dd, J = 8.8, 3.7 Hz, 1H) , 4.35 (s, 1H) , 4.18 (dd, J = 33.7, 13.8 Hz, 2H) , 3.19 –3.05 (m, 3H) , 3.00 (d, J = 16.3 Hz, 1H) , 1.88 (d, J = 13.6 Hz, 6H) , 1.76 –1.62 (m, 2H) , 1.48 (t, J = 13.0 Hz, 2H) . LCMS: m/z calculated for C₂₅H₃₀ClN₆OPS: 528.16; found: 529.81 [M+H] ⁺.

Example 37.

Synthesis of (6-amino-3- ( (5- ( (3S, 4S) -4-amino-3-methyl-2-oxa-8-azaspiro [4.5] decan-8-yl) pyrazin-2-yl) thio) -2-chlorophenyl) dimethylphosphine oxide (37)

Synthetic scheme 37

Step 1: A mixture of compound 35-6 (74 mg, 0.21 mmol) , K₂CO₃ (147 mg, 1.06 mmol) and compound 1-11 (51 mg, 0.21 mmol) in DMAc (2 mL) was stirred at 120 ℃ for 12 h. After completion of the reaction, the mixture was filtered. The filtrate was concentrated and purified by pre-HPLC (C18 column, eluted with 27%MeCN in water, TFA condition) to afford the title compound 37 (33 mg, yield 32%) as a yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ8.27 (d, J = 1.5 Hz, 1H) , 7.89 (d, J = 1.4 Hz, 1H) , 7.25 (d, J = 8.8 Hz, 1H) , 6.60 (dd, J = 8.8, 3.7 Hz, 1H) , 4.23 –4.02 (m, 3H) , 3.86 (d, J = 9.1 Hz, 1H) , 3.67 (d, J = 9.1 Hz, 1H) , 3.36 (d, J = 4.7 Hz, 1H) , 3.10 –2.97 (m, 2H) , 1.88 (d, J = 13.6 Hz, 6H) , 1.74 –1.63 (m, 3H) , 1.53 (dt, J = 13.2, 3.6 Hz, 1H) , 1.19 (d, J = 6.5 Hz, 3H) . LCMS: m/z calculated for C₂₁H₂₉ClN₅O₂PS: 481.15; found: 482.75 [M+H] ⁺.

Example 38.

Synthesis of (6-amino-3- ( (3-amino-5- ( (3S, 4S) -4-amino-3-methyl-2-oxa-8-azaspiro [4.5] decan-8-yl) pyrazin-2-yl) thio) -2-chlorophenyl) dimethylphosphine oxide (38)

Synthetic scheme 38

Step 1: A mixture of compound 36-1 (60 mg, 0.17 mmol) , K₂CO₃ (114 mg, 0.83 mmol) and compound 1-11 (41 mg, 0.17 mmol) in DMAc (2 mL) was stirred at 120 ℃ for 12 h. After completion of the reaction, the mixture was filtered. The filtrate was concentrated and purified by pre-HPLC (C18 column, eluted with 19%MeCN in water, TFA condition) . The desired components were lyophilized to afford the title compound 38 (33 mg, yield 39%) as a yellow solid. ¹HNMR (400 MHz, DMSO-d6) : δ 7.54 (s, 1H) , 6.80 (d, J = 8.9 Hz, 1H) , 6.53 (dd, J = 8.8, 3.7 Hz, 1H) , 4.23–4.01 (m, 3H) , 3.86 (d, J = 9.1 Hz, 1H) , 3.67 (d, J = 9.1 Hz, 1H) , 3.36 (d, J = 4.8 Hz, 1H) , 3.04–2.92 (m, 2H) , 1.89 (s, 3H) , 1.86 (s, 3H) , 1.71–1.61 (m, 3H) , 1.51 (d, J =13.3 Hz, 1H) , 1.19 (d, J = 6.5 Hz, 3H) . LCMS: m/z calculated for C₂₁H₃₀ClN₆O₂PS: 496.16; found: 497.91 [M+H] ⁺.

Example 39.

Synthesis of (S) - (3- ( (5- (1-amino-1, 3-dihydrospiro [indene-2, 4'-piperidin] -1'-yl) pyrazin-2-yl) thio) -2-chloro-6-methylphenyl) dimethylphosphine oxide (39)

Synthetic scheme 39

Step 1: To a solution of compound 39-1 (21.0 g, 9.01 mmol) in DMF (320 mL) was added NCS (12.1 g, 9.01 mmol) at room temperature. The reaction mixture was stirred at 40 ℃for 5 h. After completion of the reaction, the mixture was diluted with EtOAc (1 L) and washed with H₂O (1 L x 2) . The organic layer was washed with brine, dried over anhydrous Na₂SO₄, filtered and concentrated under reduced pressure. The residue was purified by silica gel column (eluted with 15%EtOAc in petroleum ether) to afford the title compound 39-2 (11.0 g, yield 46%) as a brown oil. ¹HNMR (400 MHz, DMSO-d6) : δ 6.96 (d, J = 8.1 Hz, 1H) , 6.68 (d, J = 8.1 Hz, 1H) , 2.41 (s, 3H) .

Step 2: A mixture of compound 39-2 (4.1 g, 15.36 mmol) , compound 1-7 (3.6 g, 46.07 mmol) , Pd₂ (dba) ₃ (703 mg, 0.77 mmol) , Xantphos (1.8 g, 3.07 mmol) and DIPEA (5.9 g, 46.08 mmol) in dioxane (100 mL) was stirred at 115 ℃ under nitrogen atmosphere for 16 h. After completion of the reaction, the mixture was cooled down to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 10%MeOH in DCM) to afford 2.6 g of crude product. The crude product was furtherly purified by reverse phase flash chromatography (C18 column, eluted with 35%MeCN in water, TFA condition) . The desired components were lyophilized to afford title compound 39-3 (1.9 g, yield 57%) as a yellow solid. LCMS: m/z calculated for C₉H₁₃ClNOP: 217.04; found: 218.47 [M+H] ⁺.

Step 3: A mixture of compound 39-3 (1.0 g, 4.59 mmol) and conc. HCl (1.5 mL, 18.35 mmol) in MeCN (5 mL) and H₂O (5 mL) was stirred at 0 ℃ for 30 min. A cold solution of NaNO₂ (317 mg, 4.59 mmol) in H₂O (2 mL) was added dropwise to the above mixture while keeping the temperature below 5 ℃. The mixture was stirred at 0 ℃ for 1 h before KI (1.5 g, 9.18 mmol) was added thereto. The resulting mixture was stirred at 0 ℃ for additional 1 h. The reaction mixture was extracted with EtOAc (100 mL x 2) . The combined organic layers were washed with saturated Na₂S₂O₃ solution and brine. The organic solution was dried over anhydrous Na₂SO₄, filtered and concentrated under reduced pressure. The residue was purified by silica gel column (eluted with 3%MeOH in DCM) to afford the title compound 39-4 (760 mg, yield 60%) as a yellow solid. LCMS: m/z calculated for C₉H₁₁ClIOP: 327.93; found: 329.56 [M+H] ⁺.

Step 4: A mixture of compound 39-4 (320 mg, 0.97 mmol) , compound 7-3 (329 mg, 1.95 mmol) , Pd₂ (dba) ₃ (82 mg, 0.09 mmol) , Xantphos (112 mg, 0.19 mmol) and DIPEA (375 mg, 2.91 mmol) in dioxane (100 mL) was stirred at 115 ℃ for 3 h. After completion of the reaction, the mixture was cooled down to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 83%EtOAc in petroleum ether) to afford title compound 39-5 (138 mg, yield 40%) as a yellow solid. LCMS: m/z calculated for C₁₃H₁₃Cl₂N₂OPS: 345.99; found: 347.66 [M+H] ⁺.

Step 5: A mixture of compound 39-5 (138 mg, 0.40 mmol) , K₂CO₃ (276 mg, 2.00 mmol) and 18-1 (110 mg, 0.40 mmol) in DMAc (2 mL) was stirred at 120 ℃ for 8 h. After completion of the reaction, the mixture was filtered. The filtrate was concentrated and purified by pre-HPLC (C18 column, eluted with 40%MeCN in water, TFA condition) . The desired components were lyophilized to afford the title compound 39 (13 mg, yield 6%) as a yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 8.47 (s, 1H) , 8.26 (s, 1H) , 7.50 (d, J = 7.4 Hz, 1H) , 7.40 –7.29 (m, 3H) , 7.15 (dd, J = 8.4, 2.8 Hz, 1H) , 6.93 (d, J = 8.2 Hz, 1H) , 4.41 –4.22 (m, 3H) , 3.30 –3.15 (m, 3H) , 3.02 (d, J = 16.2 Hz, 1H) , 2.65 (s, 3H) , 1.92 (s, 3H) , 1.89 (s, 3H) , 1.73 (q, J = 11.4 Hz, 2H) , 1.53 (d, J = 11.9 Hz, 2H) . LCMS: m/z calculated for C₂₆H₃₀ClN₄OPS: 512.16; found: 513.90 [M+H] ⁺.

Example 40.

Synthesis of (3- ( (5- ( (3S, 4S) -4-amino-3-methyl-2-oxa-8-azaspiro [4.5] decan-8-yl) pyrazin-2-yl) thio) -2-chloro-6-methylphenyl) dimethylphosphine oxide (40)

Synthetic scheme 40

Step 1: A mixture of compound 39-5 (60 mg, 0.17 mmol) , K₂CO₃ (119 mg, 0.86 mmol) and compound 1-11 (41 mg, 0.17 mmol) in DMAc (2 mL) was stirred at 120 ℃ for 8 h. After completion of the reaction, the mixture was filtered. The filtrate was concentrated and purified by pre-HPLC (C18 column, eluted with 49%MeCN in water, TFA condition) to afford the title compound 40 (17 mg, yield 20%) as a yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 8.46 (d, J = 1.4 Hz, 1H) , 8.25 (d, J = 1.3 Hz, 1H) , 7.14 (dd, J = 8.4, 2.8 Hz, 1H) , 6.92 (d, J = 8.2 Hz, 1H) , 4.29 –4.12 (m, 3H) , 3.89 (d, J = 9.0 Hz, 1H) , 3.69 (d, J = 9.1 Hz, 1H) , 3.21 –3.08 (m, 2H) , 2.66 (s, 3H) , 1.90 (d, J = 13.5 Hz, 6H) , 1.78 –1.66 (m, 3H) , 1.58 (d, J = 13.3 Hz, 1H) , 1.21 (d, J = 6.5 Hz, 3H) . LCMS: m/z calculated for C₂₂H₃₀ClN₄O₂PS: 480.15; found: 481.79 [M+H] ⁺.

Example 41.

Synthesis of (S) - (3- ( (5- (7-amino-5-azaspiro [2.4] heptan-5-yl) pyrazin-2-yl) thio) -2-chlorophenyl) dimethylphosphine oxide (41)

Synthetic scheme 41

Step 1: A mixture of compound 7-7 (60 mg, 0.18 mmol) , K₂CO₃ (124 mg, 0.90 mmol) and compound 41-1 (38 mg, 0.18 mmol) in DMAc (2 mL) was stirred at 100 ℃ for 3 h. After completion of the reaction, the mixture was filtered. The filtrate was concentrated and purified by reverse phase flash chromatography (C18 column, eluted with 61%MeCN in water, TFA condition) . The desired components were lyophilized to afford the title compound 41-2 (42.0 mg, yield 46%) as a yellow solid. LCMS: m/z calculated for C₂₃H₃₀ClN₄O₃PS: 508.15; found: 509.71 [M+H] ⁺.

Step 2: To a solution of compound 41-2 (42 mg, 0.18 mmol) in DCM (2 mL) was added TFA (0.6 mL) and the reaction mixture was stirred at room temperature for 1 h. After completion of the reaction, the mixture was concentrated. The residue was purified by pre-HPLC (C18 column, eluted with 30%MeCN in water, TFA condition) . The desired components were lyophilized to afford the title compound 41 (47 mg, yield 56%) as a light yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 8.34 (d, J = 1.3 Hz, 1H) , 8.10 (d, J = 1.4 Hz, 1H) , 7.74 (dd, J = 10.9, 6.8 Hz, 1H) , 7.39 (t, J = 6.9 Hz, 1H) , 7.01 (dd, J = 8.0, 1.6 Hz, 1H) , 3.93 –3.83 (m, 3H) , 3.51 (t, J = 3.1 Hz, 1H) , 3.31 (d, J = 10.8 Hz, 1H) , 1.83 (s, 3H) , 1.80 (s, 3H) , 1.11 (dt, J = 10.1, 5.3 Hz, 1H) , 0.94 –0.88 (m, 1H) , 0.87 –0.80 (m, 1H) , 0.80 –0.73 (m, 1H) . LCMS: m/z calculated for C₁₈H₂₂ClN₄OPS: 408.09; found: 409.75 [M+H] ⁺.

Example 42.

Synthesis of (2-chloro-3- ( (5- ( (1R, 3r, 5S) -3-hydroxy-8-azabicyclo [3.2.1] octan-8-yl) pyrazin-2-yl) thio) phenyl) dimethylphosphine oxide (42)

Synthetic scheme 42

Step 1: A mixture of compound 7-7 (70 mg, 0.21 mmol) , K₂CO₃ (145 mg, 1.05 mmol) and compound 42-1 (34 mg, 0.21 mmol) in DMAc (2 mL) was stirred at 100 ℃ for 3 h. After completion of the reaction, the mixture was filtered. The filtrate was concentrated and purified by pre-HPLC (C18 column, eluted with 29%MeCN in water, TFA condition) . The desired components were lyophilized to afford the title compound 42 (16 mg, yield 17%) as a light yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 8.22 (s, 2H) , 7.68 (ddd, J = 11.8, 7.6, 1.5 Hz, 1H) , 7.38 (td, J = 7.8, 1.7 Hz, 1H) , 7.06 –7.02 (m, 1H) , 4.53 (s, 2H) , 2.27 (d, J = 7.1 Hz, 2H) , 1.97 –1.87 (m, 4H) , 1.83 (s, 3H) , 1.80 (s, 3H) , 1.69 (d, J = 14.7 Hz, 2H) . LCMS: m/z calculated for C₁₉H₂₃ClN₃O₂PS: 423.09; found: 424.64 [M+H] ⁺.

Example 43.

Synthesis of (S) - (3- ( (5- (1-amino-1, 3-dihydrospiro [indene-2, 4'-piperidin] -1'-yl) -6- (hydroxymethyl) pyrazin-2-yl) thio) -2, 6-dichlorophenyl) dimethylphosphine oxide (43)

Synthetic scheme 43

Step 1: A mixture of compound 35-3 (600 mg, 1.82 mmol) and 1 N HCl (7.27 mL, 7.27 mmol) in MeCN (6 mL) and H₂O (6 mL) was stirred at 0 ℃ for 30 min. A cold solution of NaNO₂ (125.6 mg, 1.82 mmol) in H₂O (3 mL) was added dropwise to the above mixture while keeping the temperature below 5℃. The mixture was stirred at 0 ℃ for 1 h before KI (360.4 mg, 3.64 mmol) was added thereto. The reaction mixture was stirred at 0℃ for additional 1 h. The resulting mixture was extracted with EtOAc (50 mL x 2) . The combined organic layers were washed with brine, dried over anhydrous Na₂SO₄, filtered and concentrated under reduced pressure. The residue was purified by silica gel column (eluted with 3%MeOH in DCM) to afford the title compound 15-1 (580 mg, yield 91%) as a yellow solid. LCMS: m/z calculated for C₈H₈Cl₂IOP: 347.87; found: 349.52 [M+H] ⁺.

Step 2: A mixture of compound 15-1 (580 mg, 1.66 mmol) , compound 1-2 (598 mg, 4.99 mmol) , Pd₂ (dba) ₃ (152 mg, 0.16 mmol) , Xantphos (192 mg, 0.33 mmol) and DIPEA (642 mg, 4.98 mmol) in dioxane (20 mL) was stirred at 105 ℃ for 16 h. After completion of the reaction, the mixture was cooled down to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 3%MeOH in DCM) to afford 842 mg of crude product. The crude product was purified by reverse phase flash chromatography (C18 column, eluted with 63%MeCN in water, TFA condition) to afford the title compound 15-2 (553 mg, yield 97%) as a brown solid. LCMS: m/z calculated for C₁₂H₁₅Cl₂O₃PS: 339.99; found: 341.62 [M+H] ⁺.

Step 3: Under nitrogen atmosphere, to a mixture of compound 15-2 (553 mg, 1.62 mmol) in THF (8.0 mL) was added dropwise EtONa (20%in EtOH, 1.1 g, 3.24 mmol) at -30 ℃. The mixture was stirred at -30 ℃ for 1 h. After completion of the reaction, the mixture was concentrated under vacuum. The crude product was dispersed with 40 mL of cold solvent (petroleum ether/DCM=1: 1) . The insoluble precipitate was dried under vacuum after filtration to afford the title compound 15-3 (630 mg, 70 wt%) as an orange solid. LCMS: m/z calculated for C₈H₈Cl₂NaOPS: 275.93; found: 255.72 [M-Na+2H] ⁺.

Step 3: A mixture of compound 15-3 (215 mg, 70 wt%, 0.54 mmol) , compound 32-2 (210.6 mg, 0.54 mmol) , Pd₂ (dba) ₃ (45.8 mg, 0.05 mmol) , Xantphos (66 mg, 0.11 mmol) and DIPEA (278.6 mg, 2.16 mmol) in dioxane (6 mL) was stirred at 105℃ under nitrogen atmosphere for 16 h. After completion of the reaction, the mixture was cooled down to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 10%MeOH in DCM) to afford 44 mg of crude product. Then the crude product was purified by pre-HPLC (C18 column, eluted with 26%MeCN in water, TFA condition) . The desired components were lyophilized to afford the title compound 43 (26 mg, yield 8%, over 2 steps) as a light yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 8.22 (s, 1H) , 7.47 (d, J = 7.5 Hz, 1H) , 7.40 (dd, J = 8.7, 3.2 Hz, 1H) , 7.34 (d, J = 5.8 Hz, 2H) , 7.31 –7.26 (m, 1H) , 7.13 (d, J = 8.6 Hz, 1H) , 4.50 (s, 2H) , 4.34 (s, 1H) , 3.74 (d, J = 13.2 Hz, 1H) , 3.13 (dd, J = 20.4, 14.4 Hz, 3H) , 2.99 (d, J = 16.4 Hz, 1H) , 1.99 (s, 3H) , 1.96 (s, 3H) , 1.81 (d, J = 13.9 Hz, 2H) , 1.61 –1.46 (m, 2H) . LCMS: m/z calculated for C₂₆H₂₉Cl₂N₄O₂PS: 562.11; found: 563.84 [M+H] ⁺.

Example 44.

Synthesis of (S) - (3- ( (3-amino-5- (1-amino-1, 3-dihydrospiro [indene-2, 4'-piperidin] -1'-yl) pyrazin-2-yl) thio) -2-chloro-6-methylphenyl) dimethylphosphine oxide (44)

Synthetic scheme 44

Step 1: A mixture of compound 39-4 (500.0 mg, 1.52 mmol) , compound 1-2 (364.7 mg, 3.04 mmol) , Pd₂ (dba) ₃ (137.4 mg, 0.15 mmol) , Xantphos (176.0 mg, 0.30 mmol) and DIPEA (588.2 mg, 4.56 mmol) in dioxane (12 mL) was stirred at 105 ℃ under nitrogen atmosphere for 3 h. After completion of the reaction, the mixture was cooled down to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 2.5%MeOH in DCM) to afford the title compound 44-1 (290.0 mg, yield 59%) as a brown solid. LCMS: m/z calculated for C₁₃H₁₈ClO₃PS: 320.04; found: 321.59 [M+H] ⁺.

Step 2: Under nitrogen atmosphere, to a solution of compound 44-1 (290 mg, 0.90 mmol) in THF (4.0 mL) was added dropwise EtONa (20%in EtOH, 612 mg, 1.80 mmol) at -30 ℃. The mixture was stirred at -30 ℃ for 1 h. After completion of the reaction, the mixture was concentrated under vacuum. The crude product was dispersed with 30 mL of cold solvent (petroleum ether/DCM=1: 1) . The insoluble precipitate was dried under vacuum after filtration to afford the title compound 44-2 (380 mg, crude) as a yellow solid. LCMS: m/z calculated for C₉H₁₁ClNaOPS: 255.99; found: 235.70 [M-Na+2H] ⁺.

Step 3: A mixture of compound 44-2 (380 mg, crude, 0.90 mmol) , compound 1-1 (309 mg, 1.48 mmol) , Pd₂ (dba) ₃ (128 mg, 0.14 mmol) , Xantphos (171 mg, 0.30 mmol) and DIPEA (573 mg, 4.44 mmol) in dioxane (12 mL) was stirred at 105℃ under nitrogen atmosphere for 3 h. After completion of the reaction, the mixture was cooled down to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 2.5%MeOH in DCM) to afford the title compound 44-3 (150 mg, yield 46%over 2 steps) as a brown oil. LCMS: m/z calculated for C₁₃H₁₄Cl₂N₃OPS: 361.00; found: 362.55 [M+H] ⁺.

Step 4: A mixture of compound 44-3 (80 mg, 0.22 mmol) , K₂CO₃ (153 mg, 1.10 mmol) and 18-1 (61 mg, 0.22 mmol) in DMAc (2 mL) was stirred at 120℃ for 16 h. After completion of the reaction, the mixture was filtered. The filtrate was concentrated and purified by pre-HPLC (C18 column, eluted with 33%MeCN in water, TFA condition) . The desired components were lyophilized to afford the title compound 44 (31 mg, yield 26%) as a yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 7.63 (s, 1H) , 7.46 (d, J = 7.4 Hz, 1H) , 7.35 (d, J = 5.7 Hz, 2H) , 7.28 (td, J = 6.7, 5.8, 2.7 Hz, 1H) , 7.11 (dd, J = 8.4, 2.6 Hz, 1H) , 6.63 (d, J = 8.2 Hz, 1H) , 4.31 (s, 1H) , 4.21 (dd, J = 34.8, 13.4 Hz, 2H) , 3.19 –3.08 (m, 3H) , 3.01 (d, J = 16.4 Hz, 1H) , 2.58 (s, 3H) , 1.92 (s, 3H) , 1.89 (s, 3H) , 1.67 (dt, J = 12.6, 7.2 Hz, 2H) , 1.55 –1.42 (m, 2H) . LCMS: m/z calculated for C₂₆H₃₁ClN₅OPS: 527.17; found: 528.71 [M+H] ⁺.

Example 45.

Synthesis of (3- ( (3-amino-5- ( (3S, 4S) -4-amino-3-methyl-2-oxa-8-azaspiro [4.5] decan-8-yl) pyrazin-2-yl) thio) -2-chloro-6-methylphenyl) dimethylphosphine oxide (45)

Synthetic scheme 45

Step 1: A mixture of compound 44-3 (70 mg, 0.19 mmol) , K₂CO₃ (133 mg, 0.97 mmol) and compound 1-11 (46 mg, 0.19 mmol) in DMAc (2 mL) was stirred at 120 ℃ for 16 h. After completion of the reaction, the mixture was filtered. The filtrate was concentrated and purified by pre-HPLC (C18 column, eluted with 27%MeCN in water, TFA condition) to afford the title compound 45 (11 mg, yield 10%) as a yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 7.61 (s, 1H) , 7.10 (dd, J = 8.5, 2.6 Hz, 1H) , 6.62 (d, J = 8.3 Hz, 1H) , 4.23 –4.07 (m, 3H) , 3.68 (d, J = 9.3 Hz, 1H) , 3.33 (d, J = 4.7 Hz, 1H) , 3.06 –2.94 (m, 2H) , 2.58 (s, 3H) , 1.92 (s, 3H) , 1.89 (s, 3H) , 1.66 (ddd, J = 16.3, 9.9, 4.3 Hz, 3H) , 1.56 –1.50 (m, 1H) , 1.18 (d, J = 6.5 Hz, 3H) . LCMS: m/z calculated for C₂₂H₃₁ClN₅O₂PS: 495.16; found: 496.68 [M+H] ⁺.

Example 46.

Synthesis of (S) - (3- ( (5- (1-amino-1, 3-dihydrospiro [indene-2, 4'-piperidin] -1'-yl) pyrazin-2-yl) thio) -2-chloro-6-fluorophenyl) dimethylphosphine oxide (46)

Synthetic scheme 46

Step 1: To a mixture of compound 46-1 (20.0 g, 97.56 mmol) in AcOH (420 mL) was added NIS (22.0 g, 97.56 mmol) . The reaction mixture was stirred at room temperature for 16 h. After completion of the reaction, the mixture was poured into water (1 L) and extracted with EtOAc (500 mL x 2) . The combined organic layers were washed with brine, dried over anhydrous Na₂SO₄, filtered and concentrated. The crude product was purified by silica gel column (eluted with 1%EtOAc in petroleum ether) to afford the title compound 46-2 (10.0 g, yield 31%) as a light pink solid. ¹HNMR (400 MHz, DMSO-d6) : δ 7.39 (d, J = 8.8 Hz, 1H) , 6.63 (d, J = 8.8 Hz, 1H) , 5.73 (s, 2H) .

Step 2: A mixture of compound 46-2 (4.5 g, 13.55 mmol) , compound 1-7 (1.3 g, 16.26 mmol) , Pd₂ (dba) ₃ (621 mg, 0.68 mmol) , XantPhos (785 mg, 1.36 mmol) and DIPEA (5.2 g, 40.65 mmol) in dioxane (80 mL) was stirred at 100 ℃ under nitrogen atmosphere for 16 h. After completion of the reaction, the mixture was cooled down to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 3%MeOH in DCM) to afford 4.6 g of crude product. Then the crude product was purified by reverse phase flash chromatography (C18 column, eluted with 51%MeCN in water, TFA condition) . The desired components were lyophilized to afford title compound 46-3 (3.6 g, yield 94%) as a light yellow solid. LCMS: m/z calculated for C₈H₁₀BrClNOP: 280.94; found: 282.63 [M+H] ⁺.

Step 3: A mixture of compound 46-3 (3.4 g, 12.01 mmol) and 1N HCl (48 mL, 48.04 mmol) was stirred at 0 ℃ for 30 min. A cold solution of NaNO₂ (829 mg, 12.01 mmol) in H₂O (5 mL) was added dropwise to the above mixture while keeping the temperature below 5 ℃. The mixture was stirred at 0 ℃ for 1 h before HBF₄ (2.1 g, 24.02 mmol) was added thereto. The resulting mixture was stirred at 0 ℃ for additional 1 h to form an insoluble precipitate. The solid was collected by filtration and dried under vacuum. The intermediates were suspended in anhydrous toluene (50 mL) and heated at 120 ℃ for 16 h. After completion of the reaction, the mixture was concentrated under vacuum. The residue was purified by silica gel column (eluted with 3%MeOH in DCM) to afford the title compound 46-4 (893 mg, yield 26%) as a brown oil. LCMS: m/z calculated for C₈H₈BrClFOP: 283.92; found: 285.78 [M+H] ⁺.

Step 4: A mixture of compound 46-4 (893 mg, 3.13 mmol) , compound 1-2 (564 mg, 4.70 mmol) , Pd₂ (dba) ₃ (284 mg, 0.31 mmol) , Xantphos (362 mg, 0.63 mmol) and DIPEA (1.2 g, 9.39 mmol) in dioxane (20.0 mL) was stirred at 105 ℃ under nitrogen atmosphere for 16 h. After completion of the reaction, the mixture was cooled down to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 5%MeOH in DCM) to afford title compound 46-5 (902 mg, yield 89%) as a yellow oil. LCMS: m/z calculated for C₁₂H₁₅ClFO₃PS: 324.02; found: 325.78 [M+H] ⁺.

Step 5: Under nitrogen atmosphere, to a solution of compound 46-5 (902 mg, 2.78 mmol) in THF (10.0 mL) was added dropwise EtONa (20%in EtOH, 1.9 g, 5.55 mmol) at -75 ℃. The mixture was stirred at -75 ℃ for 1 h. After completion of the reaction, the mixture was concentrated under vacuum. The crude product was dispersed with 100 mL of cold solvent (petroleum ether/DCM=1: 1) . The insoluble precipitate was dried under vacuum after filtration to afford the title compound 46-6 (1.0 g, crude, 72 wt%) as a yellow solid. LCMS: m/z calculated for C₈H₈ClFNaOPS: 259.96; found: 239.47 [M-Na+2H] ⁺.

Step 6: A mixture of compound 46-6 (500 mg, 72 wt%, 1.38 mmol) , compound 46-7 (503 mg, 2.87 mmol) , Pd₂ (dba) ₃ (82 mg, 0.09 mmol) , Pd (dppf) Cl₂ (66 mg, 0.09 mmol) , Xantphos (222.3 mg, 0.38 mmol) and DIPEA (743.0 mg, 5.76 mmol) in dioxane (20.0 mL) was stirred at 105 ℃ under nitrogen atmosphere for 2 h. After completion of the reaction, the mixture was cooled down to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 10%MeOH in DCM) to afford 311 mg of crude product. Then the crude product was purified by reverse phase flash chromatography (C18 column, eluted with 20%MeCN in water, TFA condition) to afford title compound 46-8 (167 mg, yield 36%over 2 steps) as a yellow solid. LCMS: m/z calculated for C₁₂H₁₁ClFN₂O₂PS: 332.00; found: 333.53 [M+H] ⁺.

Step 7: To a mixture of compound 46-8 (70 mg, 0.21 mmol) , DBU (192 mg, 1.26 mmol) and compound 18-1 (58 mg, 0.21 mmol) in anhydrous DMF (5 mL) was added BOP (279 mg, 0.63 mmol) . The mixture was stirred at room temperature for 1 h. After completion of the reaction, the mixture was filtered. The filtrate was purified by pre-HPLC (C18 column, eluted with 30%MeCN in water, TFA condition) . The desired components were lyophilized to afford the title compound 46 (30 mg, yield 28%) as a white solid. ¹H NMR (400 MHz, DMSO-d₆) δ 8.39 (d, J = 1.1 Hz, 1H) , 8.23 (d, J = 1.2 Hz, 1H) , 7.47 (d, J = 7.4 Hz, 1H) , 7.35 (d, J = 6.0 Hz, 2H) , 7.28 (dd, J = 12.9, 4.3 Hz, 1H) , 7.24 –7.16 (m, 2H) , 4.31 (d, J = 14.6 Hz, 2H) , 4.21 (d, J = 13.6 Hz, 1H) , 3.27 –3.11 (m, 3H) , 3.02 (d, J = 16.4 Hz, 1H) , 1.88 (dd, J = 13.8, 3.8 Hz, 6H) , 1.70 (q, J = 9.0 Hz, 2H) , 1.52 (t, J = 14.2 Hz, 2H) . LCMS: m/z calculated for C₂₅H₂₇ClFN₄OPS: 516.13; found: 517.67 [M+H] ⁺.

Example 47.

Synthesis of (S) - (3- ( (5- (1-amino-1, 3-dihydrospiro [indene-2, 4'-piperidin] -1'-yl) pyrazin-2-yl) thio) -2, 6-dichlorophenyl) dimethylphosphine oxide (47)

Synthetic scheme 47

Step 1: A mixture of compound 15-3 (485 mg, 72 wt%, 1.26 mmol) , compound 7-1 (243 mg, 1.26 mmol) , Pd₂ (dba) ₃ (110 mg, 0.12 mmol) , Xantphos (146 mg, 0.25 mmol) and DIPEA (488 mg, 3.78 mmol) in dioxane (8.0 mL) was stirred at 105 ℃ under nitrogen atmosphere for 16 h. After completion of the reaction, the mixture was cooled down to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 5%MeOH in DCM) to afford title compound 47-1 (100 mg, yield 22%) as a brown solid. LCMS: m/z calculated for C₁₂H₁₀Cl₃N₂OPS: 365.93; found: 367.88 [M+H] ⁺.

Step 2: A mixture of compound 47-1 (100 mg, 0.27 mmol) , DIPEA (351 mg, 2.70 mmol) and compound 18-1 (74 mg, 0.27 mmol) in NMP (5 mL) was stirred at 120 ℃ for 3 h. After completion of the reaction, the mixture was filtered and concentrated. The residue was purified by pre-HPLC (C18 column, eluted with 30%MeCN in water, TFA condition) to afford the title compound 47 (10 mg, yield 7%) as a light yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 8.42 (s, 1H) , 8.26 (s, 1H) , 7.46 (d, J = 7.4 Hz, 1H) , 7.39 (dd, J = 8.7, 3.2 Hz, 1H) , 7.35 (d, J = 5.5 Hz, 2H) , 7.29 (dd, J = 9.0, 4.2 Hz, 1H) , 6.95 (d, J = 8.7 Hz, 1H) , 4.36 –4.28 (m, 2H) , 4.23 (d, J = 14.5 Hz, 1H) , 3.29 –3.11 (m, 3H) , 3.02 (d, J = 16.4 Hz, 1H) , 1.97 (d, J = 13.6 Hz, 6H) , 1.76 –1.64 (m, 2H) , 1.59 –1.47 (m, 2H) . LCMS: m/z calculated for C₂₅H₂₇Cl₂N₄OPS: 532.10; found: 533.58 [M+H] ⁺.

Example 48.

Synthesis of (S) - (3- ( (5- (1-amino-1, 3-dihydrospiro [indene-2, 4'-piperidin] -1'-yl) pyrazin-2-yl) thio) -2-chloro-6-methoxyphenyl) dimethylphosphine oxide (48)

Synthetic scheme 48

Step 1: A mixture of compound 35-3 (1.4 g, 4.24 mmol) and 1 N H₂SO₄ (17 mL, 16.96 mmol) was stirred at 0 ℃ for 30 min. A cold solution of NaNO₂ (288 mg, 4.24 mmol) in H₂O (3 mL) was added dropwise to the above mixture while keeping the temperature below 5 ℃. The reaction mixture was stirred at 0 ℃ for 0.5 h and 100 ℃ for 4 h. After completion of the reaction, the mixture was extracted with EtOAc (100 mL x 2) and H₂O (100 mL) . The combined organic layers were washed with brine, dried over anhydrous Na₂SO₄, filtered and concentrated under reduced pressure. The residue was purified by silica gel column (eluted with 2.5%MeOH in DCM) to afford the title compound 48-1 (1.2 g, yield 88%) as a light yellow solid. LCMS: m/z calculated for C₈H₉ClIO₂P: 329.91; found: 331.47 [M+H] ⁺.

Step 2: To a mixture of compound 48-1 (640 mg, 1.94 mmol) and K₂CO₃ (803 mg, 5.82 mmol) in DMF (10 mL) was added MeI (413 mg, 2.91 mmol) . The mixture was stirred at 80 ℃ for 2 h. After completion of the reaction, the mixture was filtered. The filtrate was concentrated and purified by reverse phase flash chromatography (C18 column, eluted with 80%MeCN in water, TFA condition) . The desired components were lyophilized to afford the title compound 48-2 (480 mg, yield 72%) as a yellow solid. LCMS: m/z calculated for C₉H₁₁ClIO₂P: 343.92; found: 345.67 [M+H] ⁺.

Step 3: A mixture of compound 48-2 (400 mg, 1.16 mmol) , compound 1-2 (417 mg, 3.48 mmol) , Pd₂ (dba) ₃ (101 mg, 0.11 mmol) , Xantphos (134 mg, 0.23 mmol) and DIPEA (449 mg, 3.48 mmol) in dioxane (15 mL) was stirred at 105 ℃ under nitrogen atmosphere for 16 h. After completion of the reaction, the mixture was cooled down to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 5%MeOH in DCM) to afford title compound 48-3 (350 mg, yield 90%) as a brown oil. LCMS: m/z calculated for C₁₃H₁₈ClO₄PS: 336.04; found: 337.66 [M+H] ⁺.

Step 4: Under nitrogen atmosphere, to a mixture of compound 48-3 (350 mg, 1.04 mmol) in THF (10 mL) was added dropwise EtONa (20%in EtOH, 706 mg, 2.08 mmol) at -30 ℃. The mixture was stirred at -30 ℃ for 1 h. After completion of the reaction, the mixture was concentrated under vacuum. The crude product was dispersed with 100 mL of cold solvent (petroleum ether/DCM=1: 1) . The insoluble precipitate was dried under vacuum after filtration to afford the title compound 48-4 (380 mg, crude) as a yellow solid. LCMS: m/z calculated for C₉H₁₁ClNaO₂PS: 271.98; found: 251.69 [M-Na+2H] ⁺.

Step 5: A mixture of compound 48-4 (380 mg, crude, 1.04 mmol) , compound 7-1 (267 mg, 1.39 mmol) , Pd₂ (dba) ₃ (119 mg, 0.13 mmol) , Xantphos (161 mg, 0.28 mmol) and DIPEA (538 mg, 4.17 mmol) in dioxane (12 mL) was stirred at 105 ℃ under nitrogen atmosphere for 2 h. After completion of the reaction, the mixture was cooled down to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 6%MeOH in DCM) to afford title compound 48-5 (220 mg, yield 44%over 2 steps) as a yellow solid. LCMS: m/z calculated for C₁₃H₁₃Cl₂N₂O₂PS: 361.98; found: 363.51 [M+H] ⁺.

Step 6: A mixture of compound 48-5 (100 mg, 0.28 mmol) , K₂CO₃ (190 mg, 1.38 mmol) and compound 18-1 (77 mg, 0.28 mmol) in DMAc (3 mL) was stirred at 80 ℃ for 4 h. After completion of the reaction, the mixture was filtered. The filtrate was concentrated and purified by pre-HPLC (C18 column, eluted with 28%MeCN in water, TFA condition) to afford the title compound 48 (58 mg, yield 40%) as a yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ8.33 (d, J = 1.2 Hz, 1H) , 8.11 (d, J = 1.3 Hz, 1H) , 7.46 (d, J = 7.5 Hz, 1H) , 7.38 –7.26 (m, 4H) , 7.08 (dd, J = 9.0, 4.3 Hz, 1H) , 4.31 (s, 1H) , 4.27 (d, J = 14.0 Hz, 1H) , 4.17 (d, J = 13.6 Hz, 1H) , 3.80 (s, 3H) , 3.16 (dd, J = 19.3, 14.1 Hz, 3H) , 3.01 (d, J = 16.3 Hz, 1H) , 1.80 (s, 3H) , 1.76 (s, 3H) , 1.66 (dd, J = 17.1, 8.1 Hz, 2H) , 1.50 (t, J = 13.9 Hz, 2H) . LCMS: m/z calculated for C₂₆H₃₀ClN₄O₂PS: 528.15; found: 529.81 [M+H] ⁺.

Example 49.

Synthesis of (S) - (3- ( (5- (1-amino-1, 3-dihydrospiro [indene-2, 4'-piperidin] -1'-yl) pyrazin-2-yl) thio) -2-chloro-6-hydroxyphenyl) dimethylphosphine oxide (49)

Synthetic scheme 49

Step 1: Under nitrogen atmosphere, to a solution of compound 48 (22 mg, 0.04 mmol) in DCM (1 mL) was added BBr₃ (1 M in DCM, 0.12 mL, 0.12 mmol) at -30 ℃. The mixture was stirred for 3 h. After completion of the reaction, the reaction was quenched with H₂O (1 mL) at 0 ℃ and concentrated under reduced pressure. The residue was purified by pre- HPLC (C18 column, eluted with 40%MeCN in water, TFA condition) . The desired components were lyophilized to afford the title compound 49 (16 mg, yield 74%) as an off-white solid. ¹H NMR (400 MHz, DMSO-d₆) δ 8.28 (d, J = 1.2 Hz, 1H) , 8.06 (d, J = 1.3 Hz, 1H) , 7.45 (d, J = 7.4 Hz, 1H) , 7.38 –7.25 (m, 4H) , 6.80 (dd, J = 8.9, 3.7 Hz, 1H) , 4.30 (s, 1H) , 4.23 (d, J = 13.6 Hz, 1H) , 4.14 (d, J = 14.1 Hz, 1H) , 3.23 –3.09 (m, 3H) , 3.00 (d, J = 16.4 Hz, 1H) , 1.98 (d, J = 13.9 Hz, 6H) , 1.72 –1.61 (m, 2H) , 1.55 –1.43 (m, 2H) . LCMS: m/z calculated for C₂₅H₂₈ClN₄O₂PS: 514.14; found: 515.75 [M+H] ⁺.

Example 50.

Synthesis of (S) - (3- ( (5- (1-amino-1, 3-dihydrospiro [indene-2, 4'-piperidin] -1'-yl) pyrazin-2-yl) thio) -2-chloro-6- (pyrrolidin-1-yl) phenyl) dimethylphosphine oxide (50)

Synthetic scheme 50

Step 1: To a mixture of compound 46-8 (100 mg, 0.30 mmol) in THF (1 mL) was added pyrrolidine (107 mg, 1.5 mmol) at room temperature. Then the mixture was stirred at 80 ℃ for 1 h. After completion of the reaction, the mixture was concentrated under vacuum. The crude product was purified by Pre-TLC (eluted with 10%MeOH in DCM) to afford the title compound 50-1 (30 mg, yield 26%) as a brown solid. LCMS: m/z calculated for C₁₆H₁₉ClN₃O₂PS: 383.06; found: 384.64 [M+H] ⁺.

Step 2: To a mixture of compound 50-1 (30 mg, 0.08 mmol) , DBU (73 mg, 0.48 mmol) and compound 18-1 (25 mg, 0.09 mmol) in DMF (2 mL) was added BOP (106 mg, 0.24 mmol) . The mixture was stirred at room temperature for 1 h. After completion of the reaction, the mixture was filtered and concentrated. The residue was purified by pre-HPLC (C18 column, eluted with 36%MeCN in water, TFA condition) to afford the title compound 50 (7 mg, yield 15%) as a white solid. ¹H NMR (400 MHz, DMSO-d₆) δ 8.36 (s, 1H) , 8.19 (s, 1H) , 7.45 (d, J = 7.5 Hz, 1H) , 7.35 (d, J = 6.1 Hz, 2H) , 7.31 –7.25 (m, 1H) , 7.20 (d, J = 8.9 Hz, 1H) , 4.28 (d, J = 12.7 Hz, 2H) , 4.18 (d, J = 12.9 Hz, 1H) , 3.45 (s, 4H) , 3.21 (d, J = 12.4 Hz, 2H) , 3.13 (d, J = 16.4 Hz, 1H) , 3.02 (d, J = 16.4 Hz, 1H) , 2.05 (d, J = 13.2 Hz, 10H) , 1.68 (t, J = 11.7 Hz, 2H) , 1.52 (dd, J = 25.1, 12.9 Hz, 2H) . LCMS: m/z calculated for C₂₉H₃₅ClN₅OPS: 567.20; found: 568.85 [M+H] ⁺.

Example 51.

Synthesis of (S) -4- ( (5- (1-amino-1, 3-dihydrospiro [indene-2, 4'-piperidin] -1'-yl) pyrazin-2-yl) thio) -3-chloro-2- (dimethylphosphoryl) benzonitrile (51)

Synthetic scheme 51

Step 1: A mixture of compound 46-3 (1.0 g, 3.53 mmol) and 1 N HCl (14 mL, 14.13 mmol) in MeCN (5 mL) was stirred at 0 ℃ for 0.5 h. A cold solution of NaNO₂ (244 mg, 3.53 mmol) in H₂O (2 mL) was added dropwise to the above mixture while keeping the temperature below 5 ℃. The reaction mixture was stirred at 0 ℃ for 1 h before KI (1.2 g, 7.06 mmol) was added thereto. The mixture was stirred at 0 ℃ for additional 1 h. After completion of the reaction, the mixture was diluted with H₂O (50 mL) and extracted with EtOAc (100 mL x 2) . The combined organic layers were washed with brine, dried over anhydrous Na₂SO₄, filtered and concentrated under reduced pressure. The residue was purified by silica gel column (eluted with 5%MeOH in DCM) to afford the title compound 51-1 (1.2 g, yield 86%) as an orange solid. LCMS: m/z calculated for C₈H₈BrClIOP: 391.82; found: 393.29 [M+H] ⁺.

Step 2: A mixture of compound 51-1 (1.1 g, 2.80 mmol) , Pd (PPh₃) ₄ (324 mg, 0.28 mmol) and Zn (CN) ₂ (360 mg, 3.08 mmol) in DMF (10 mL) was stirred at 100 ℃ under nitrogen atmosphere for 24 h. After completion of the reaction, the mixture was cooled down to room temperature and filtered. The filtrate was concentrated and purified by reverse phase flash chromatography (C18 column, eluted with 49%MeCN in water, HCl condition) . The desired components were lyophilized to afford title compound 51-2 (546 mg, yield 67%) as a light yellow solid. LCMS: m/z calculated for C₉H₈BrClNOP: 290.92; found: 292.51 [M+H] ⁺.

Step 3: A mixture of compound 51-2 (360 mg, 1.23 mmol) , 1-2 (295 mg, 2.46 mmol) , Pd₂ (dba) ₃ (110 mg, 0.12 mmol) , Xantphos (139 mg, 0.24 mmol) and DIPEA (476 mg, 3.69 mmol) in dioxane (10 mL) was stirred at 105 ℃ under nitrogen atmosphere for 2 h. After completion of the reaction, the mixture was cooled down to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 3%MeOH in DCM) to afford title compound 51-3 (350 mg, yield 86%) as a yellow oil. LCMS: m/z calculated for C₁₃H₁₅ClNO₃PS: 331.02; found: 332.57 [M+H] ⁺.

Step 4: Under nitrogen atmosphere, to a mixture of compound 51-3 (350 mg, 1.05 mmol) in THF (10 mL) was added dropwise EtONa (20%in EtOH, 717 mg, 2.10 mmol) at -75 ℃. The mixture was stirred at -75 ℃ for 1 h. After completion of the reaction, the mixture was concentrated under vacuum. The crude product was dispersed with 100 mL of cold solvent (petroleum ether/DCM=1: 1) . The insoluble precipitate was dried under vacuum after filtration to afford the title compound 51-4 (430 mg, crude) as an orange solid. LCMS: m/z calculated for C₉H₈ClNNaOPS: 266.97; found: 246.68 [M-Na+2H] ⁺.

Step 5: A mixture of compound 51-4 (430 mg, crude) , compound 7-1 (372 mg, 1.93 mmol) , Pd₂ (dba) ₃ (147 mg, 0.16 mmol) , Xantphos (185 mg, 0.32 mmol) and DIPEA (619 mg, 4.80 mmol) in dioxane (13 mL) was stirred at 105 ℃ under nitrogen atmosphere for 2 h. After completion of the reaction, the mixture was cooled down to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 5%MeOH in DCM) to afford title compound 51-5 (150 mg, yield 40%over 2 steps) as a yellow solid. LCMS: m/z calculated for C₁₃H₁₀Cl₂N₃OPS: 356.97; found: 358.57 [M+H] ⁺.

Step 6: A mixture of compound 51-5 (150 mg, 0.42 mmol) , DIPEA (542 mg, 4.2 mmol) and 18-1 (127 mg, 0.46 mmol) in DMAc (3 mL) was stirred at 100 ℃ for 4 h. After completion of the reaction, the mixture was filtered and concentrated. The residue was purified by pre-HPLC (C18 column, eluted with 30%MeCN in water, H₂O condition) . The desired components were lyophilized to afford the title compound 51 (9 mg, yield 4%) as a light yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 8.42 (d, J = 1.4 Hz, 1H) , 8.29 (d, J = 1.3 Hz, 1H) , 7.72 (dd, J = 8.4, 2.2 Hz, 1H) , 7.45 (d, J = 7.5 Hz, 1H) , 7.35 (d, J = 2.6 Hz, 2H) , 7.31 –7.26 (m, 1H) , 7.04 (d, J = 8.4 Hz, 1H) , 4.32 (d, J = 17.2 Hz, 2H) , 4.23 (d, J = 13.7 Hz, 1H) , 3.25 (q, J = 11.5 Hz, 2H) , 3.17 –3.00 (m, 2H) , 1.96 (s, 3H) , 1.93 (s, 3H) , 1.69 (t, J = 11.7 Hz, 2H) , 1.53 (dd, J = 23.6, 13.7 Hz, 2H) . LCMS: m/z calculated for C₂₆H₂₇ClN₅OPS: 523.14; found: 524.87 [M+H] ⁺.

Example 52.

Synthesis of (S) -4- ( (5- (1-amino-1, 3-dihydrospiro [indene-2, 4'-piperidin] -1'-yl) pyrazin-2-yl) thio) -3-chloro-2- (dimethylphosphoryl) benzamide (52)

Synthetic scheme 52

Step 1: A mixture of compound 51-2 (186 mg, 0.63 mmol) , compound 1-2 (151 mg, 1.26 mmol) , Pd₂ (dba) ₃ (55 mg, 0.06 mmol) , Xantphos (73 mg, 0.12 mmol) and DIPEA (244 mg, 1.89 mmol) in dioxane (5 mL) was stirred at 120 ℃ under nitrogen atmosphere for 16 h. After completion of the reaction, the mixture was cooled down to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 5%MeOH in DCM) to afford title compound 52-1 (155 mg, yield 70%) as a yellow oil. LCMS: m/z calculated for C₁₃H₁₇ClNO₄PS: 349.03; found: 350.61 [M+H] ⁺.

Step 2: To a mixture of compound 52-1 (155 mg, 0.44 mmol) in THF (5 mL) was added dropwise EtONa (20%in EtOH, 299 mg, 0.88 mmol) at -75 ℃. The mixture was stirred at -75 ℃ for 1 h. After completion of the reaction, the mixture was concentrated under vacuum. The crude product was dispersed with 70 mL of cold solvent (petroleum ether/DCM=1: 1) . The insoluble precipitate was dried under vacuum after filtration to afford the title compound 52-2 (170 mg, crude) as an orange solid. LCMS: m/z calculated for C₉H₁₀ClNNaO₂PS: 284.98; found: 264.65 [M-Na+2H] ⁺.

Step 3: A mixture of compound 52-2 (170 mg, crude, 0.44 mmol) , compound 7-1 (149 mg, 0.77 mmol) , Pd₂ (dba) ₃ (46 mg, 0.05 mmol) , Xantphos (58 mg, 0.10 mmol) and DIPEA (228 mg, 1.77 mmol) in dioxane (6 mL) was stirred at 105 ℃ under nitrogen atmosphere for 2 h. After completion of the reaction, the mixture was cooled down to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 10%MeOH in DCM) to afford title compound 52-3 (45 mg, yield 27%over 2 steps) as a yellow solid. LCMS: m/z calculated for C₁₃H₁₂Cl₂N₃O₂PS: 374.98; found: 376.62 [M+H] ⁺.

Step 4: A mixture of compound 52-3 (45 mg, 0.12 mmol) , DIPEA (155 mg, 1.20 mmol) and 18-1 (36 mg, 0.13 mmol) in DMAc (2 mL) was stirred at 120 ℃ for 4 h. After completion of the reaction, the mixture was filtered and concentrated. The residue was purified by pre-HPLC (C18 column, eluted with 24%MeCN in water, TFA condition) . The desired components were lyophilized to afford the title compound 52 (19 mg, yield 29%) as a light yellow solid. LCMS: m/z calculated for C₂₆H₂₉ClN₅O₂PS: 541.15; found: 542.85 [M+H] ⁺. ¹H NMR (400 MHz, DMSO-d₆) δ 8.40 (d, J = 1.4 Hz, 1H) , 8.26 (d, J = 1.3 Hz, 1H) , 7.45 (d, J = 7.5 Hz, 1H) , 7.34 (d, J = 3.2 Hz, 2H) , 7.31 –7.26 (m, 1H) , 7.18 (dd, J = 8.2, 2.4 Hz, 1H) , 6.99 (d, J = 8.1 Hz, 1H) , 4.32 (d, J = 8.7 Hz, 2H) , 4.21 (d, J = 13.4 Hz, 1H) , 3.23 (q, J = 11.7, 11.3 Hz, 2H) , 3.16 –2.98 (m, 2H) , 1.87 (d, J = 13.7 Hz, 6H) , 1.69 (t, J = 12.7 Hz, 2H) , 1.53 (dd, J = 21.8, 13.2 Hz, 2H) .

Example 53.

Synthesis of (S) - (3- ( (5- (1-amino-1, 3-dihydrospiro [indene-2, 4'-piperidin] -1'-yl) pyrazin-2-yl) thio) -2-chloro-6- (1H-1, 2, 3-triazol-1-yl) phenyl) dimethylphosphine oxide (53)

Synthetic scheme 53

Step 1: Under nitrogen atmosphere, to a solution of compound 46-3 (1.0 g, 3.53 mmol) in MeCN (10 mL) was added tert-butyl nitrite (546 mg, 5.30 mmol) and azidotrimethylsilane (610 mg, 5.30 mmol) at 0 ℃. The mixture was stirred at room temperature for 3 h. After completion of the reaction, the mixture was extracted with EtOAc (50 mL x 2) and H₂O (50 mL) . The combined organic layers were washed with brine, dried over anhydrous Na₂SO₄, filtered and concentrated under reduced pressure. The residue was purified by silica gel column (eluted with 1.6%MeOH in DCM) to afford the title compound 53-1 (920 mg, yield 92%) as a yellow solid. LCMS: m/z calculated for C₈H₈BrClN₃OP: 306.93; found: 308.70 [M+H] ⁺.

Step 2: A mixture of compound 53-1 (820 mg, 2.65 mmol) , trimethylsilylacetylene (338 mg, 3.45 mmol) , CuI (504 mg, 2.65 mmol) and DIPEA (1.4 g, 10.60 mmol) in DMF (15 mL) was stirred at room temperature under nitrogen atmosphere for 5 h. After completion of the reaction, the mixture was extracted with EtOAc (80 mL x 2) and H₂O (80 mL) . The combined organic layers were washed with brine, dried over anhydrous Na₂SO₄, filtered and concentrated under reduced pressure. The mixture was purified by silica gel column (eluted with 5%MeOH in DCM) to afford title compound 53-2 (436 mg, yield 40%) as a yellow solid. LCMS: m/z calculated for C₁₃H₁₈BrClN₃OPSi: 404.98; found: 406.59 [M+H] ⁺.

Step 3: A mixture of compound 53-2 (436 mg, 1.07 mmol) , LiOH (51 mg, 2.14 mmol) in MeOH (5 mL) was stirred at room temperature for 6 h. After completion of the reaction, the mixture was adjusted pH to 7 with 1N HCl. The resulting mixture was extracted with EtOAc (50 mL x 2) and H₂O (50 mL) . The combined organic layers were washed with brine, dried over anhydrous Na₂SO₄ and concentrated under reduced pressure. The mixture was purified by silica gel column (eluted with 5%MeOH in DCM) to afford title compound 53-3 (118 mg, yield 33%) as a yellow solid. ¹HNMR (400 MHz, DMSO-d6) : δ 8.50 (s, 1H) , 8.18 (d, J = 8.5 Hz, 1H) , 7.90 (s, 1H) , 7.42 (dd, J = 8.5, 2.9 Hz, 1H) , 1.64 (s, 3H) , 1.61 (s, 3H) .

Step 4: A mixture of compound 53-3 (180 mg, 0.54 mmol) , compound 1-2 (129 mg, 1.07 mmol) , Pd₂ (dba) ₃ (46 mg, 0.05 mmol) , Xantphos (58 mg, 0.10 mmol) and DIPEA (209 mg, 1.62 mmol) in dioxane (4 mL) was stirred at 105 ℃ under nitrogen atmosphere for 2 h. After completion of the reaction, the mixture was cooled down to room temperature and concentrated under vacuum. The residue was purified by silica gel column (eluted with 5%MeOH in DCM) to afford title compound 53-4 (180 mg, yield 89%) as a yellow solid. LCMS: m/z calculated for C₁₄H₁₇ClN₃O₃PS: 373.04; found: 374.63 [M+H] ⁺.

Step 5: Under nitrogen atmosphere, to a mixture of compound 53-4 (180 mg, 0.48 mmol) in THF (3 mL) was added dropwise EtONa (20%in EtOH, 327 mg, 0.96 mmol) at -30 ℃. The mixture was stirred at -30 ℃ for 1 h. After completion of the reaction, the mixture was concentrated under vacuum. The crude product was dispersed with 30 mL of cold solvent (petroleum ether/DCM=1: 1) . The insoluble precipitate was dried under vacuum after filtration to afford the title compound 53-5 (170 mg, crude) as an orange solid. LCMS: m/z calculated for C₁₀H₁₀ClN₃NaOPS: 308.99; found: 288.60 [M-Na+2H] ⁺.

Step 6: A mixture of compound 53-5 (170 mg, crude, 0.48 mmol) , compound 7-1 (106 mg, 0.55 mmol) , Pd₂ (dba) ₃ (46 mg, 0.05 mmol) , Xantphos (58mg, 0.10 mmol) and DIPEA (213 mg, 1.65 mmol) in dioxane (3 mL) was stirred at 105 ℃ under nitrogen atmosphere for 2 h. After completion of the reaction, the mixture was cooled down to room temperature and concentrated under vacuum. The residue was purified by Pre-TLC (eluted with 3%MeOH in DCM) to afford title compound 53-6 (54 mg, yield 28%over 2 steps) as a brown solid. LCMS: m/z calculated for C₁₄H₁₂Cl₂N₅OPS: 398.99; found: 400.56 [M+H] ⁺.

Step 7: A mixture of compound 53-6 (54 mg, 0.14 mmol) , DIPEA (174 mg, 1.40 mmol) and 18-1 (46 mg, 0.17 mmol) in NMP (2 mL) was stirred at 120 ℃ for 3 h. After completion of the reaction, the mixture was filtered and concentrated. The residue was purified by pre-HPLC (C18 column, eluted with 49%MeCN in water, TFA condition) to afford the title compound 53 (30 mg, yield 39%) as a light yellow solid. ¹H NMR (400 MHz, DMSO-d₆) δ 8.40 (d, J = 1.4 Hz, 1H) , 8.30 (s, 2H) , 7.84 (d, J = 1.2 Hz, 1H) , 7.44 (d, J = 7.5 Hz, 1H) , 7.35 (d, J = 5.9 Hz, 2H) , 7.27 (td, J = 8.7, 8.1, 3.0 Hz, 2H) , 7.11 (d, J = 8.5 Hz, 1H) , 4.31 (d, J = 13.5 Hz, 2H) , 4.21 (d, J = 13.6 Hz, 1H) , 3.23 (q, J = 11.6 Hz, 2H) , 3.16 –2.99 (m, 2H) , 1.70 (d, J = 9.1 Hz, 2H) , 1.61 (d, J = 13.8 Hz, 8H) . LCMS: m/z calculated for C₂₇H₂₉ClN₇OPS: 565.16; found: 566.86 [M+H] ⁺.

Example 54.

Enzymatic activity assay of SHP2

Materials:

· SHP2 (BPS, Cat. No. 79018)

· SHP2 Activating Peptide (BPS, Cat. No. 79319-2)

· OptiPlate TM-384 F black assay plates (Perkin Elmer, Cat. No. 6007279)

· DiFMUP (Invitrogen, Cat. No. D6567)

· Compounds were dissolved into 10 mM stock in 100%DMSO

Experimental methods:

○ Prepare 1x assay buffer

○ Prepare 1x assay buffer (modified Hepes Buffer) .

○ Compound serial dilution:

○ Transfer 200nL compounds to assay plate by Echo in 100%DMSO. The final fraction of DMSO is 1%.

○ Prepare 4x SHP-2 activating peptide solution:

○ Add SHP-2 activating Peptide in 1x assay buffer to make 4x SHP-2 activiting peptide

○ solution. Transfer 5uL 4x SHP-2 activating peptide solution to assay plate. Centrifuge at 1000 rpm for 1 min.

○ Prepare 4x SHP-2 enzyme solution:

○ Add SHP-2 in 1x assay buffer to make 4x SHP-2 enzyme solution. Transfer 5uL 4x SHP-2 enzyme solution to assay plate, except for the negative wells, transfer 5uL 1x assay buffer instead. Centrifuge at 1000 rpm for 1 min. Incubate at RT for 60min.

○ Prepare 2x substrate solution:

○ Add DiFMUP in 1x assay buffer to make the substrate solution. Transfer 10 μL of 2x substrate solution to each well to start reaction. Centrifuge at 1000 rpm for 1 min.

○ Read the plate kinetically on Paradigm for 60 min with excitation at 360nm and emission at 460nm.

○ Curve fitting

○ Fit the data in Excel to obtain inhibition values using equation (1)

○ Equation (1) : Inh %= (Max-Signal) / (Max-Min) *100

○ Fit the data in XL-Fit to obtain IC50 values using equation (2)

○ Equation (2) : Y=Bottom + (Top-Bottom) / (1+ (IC50/X) *HillSlope)

○ Y is %inhibition and X is compound concentration.

IC₅₀ data from SHP2 assay testing is shown in Table 4.

Table 4

A: < 1 μM; B: 1 –10 μM. C: > 10 μM

Applicant’s disclosure is described herein in preferred embodiments with reference to the Figures, in which like numbers represent the same or similar elements. Reference throughout this specification to “one embodiment, ” “an embodiment, ” or similar language means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment of the present invention. Thus, appearances of the phrases “in one embodiment, ” “in an embodiment, ” and similar language throughout this specification may, but do not necessarily, all refer to the same embodiment.

The described features, structures, or characteristics of Applicant’s disclosure may be combined in any suitable manner in one or more embodiments. In the description, herein, numerous specific details are recited to provide a thorough understanding of embodiments of the invention. One skilled in the relevant art will recognize, however, that Applicant’s composition and/or method may be practiced without one or more of the specific details, or with other methods, components, materials, and so forth. In other instances, well-known structures, materials, or operations are not shown or described in detail to avoid obscuring aspects of the disclosure.

In this specification and the appended claims, the singular forms "a, " "an, " and "the" include plural reference, unless the context clearly dictates otherwise.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. Although any methods and materials similar or equivalent to those described herein can also be used in the practice or testing of the present disclosure, the preferred methods and materials are now described. Methods recited herein may be carried out in any order that is logically possible, in addition to a particular order disclosed.

Incorporation by Reference

References and citations to other documents, such as patents, patent applications, patent publications, journals, books, papers, web contents, have been made in this disclosure. All such documents are hereby incorporated herein by reference in their entirety for all purposes. Any material, or portion thereof, that is said to be incorporated by reference herein, but which conflicts with existing definitions, statements, or other disclosure material explicitly set forth herein is only incorporated to the extent that no conflict arises between that incorporated material and the present disclosure material. In the event of a conflict, the conflict is to be resolved in favor of the present disclosure as the preferred disclosure.

Equivalents

The representative examples are intended to help illustrate the invention, and are not intended to, nor should they be construed to, limit the scope of the invention. Indeed, various modifications of the invention and many further embodiments thereof, in addition to those shown and described herein, will become apparent to those skilled in the art from the full contents of this document, including the examples and the references to the scientific and patent literature included herein. The examples contain important additional information, exemplification and guidance that can be adapted to the practice of this invention in its various embodiments and equivalents thereof.

Claims

A compound having the structural formula of (I) :

wherein

X is S or a single bond;

Y¹ is CR⁷ or N;

Y² is CR⁸ or N, provided that if one of Y¹ and Y² is N, the other is not N;

Ring A is a unsubstituted or substituted, 5-or 6-membered heterocyclic ring or a 7-to 14-membered bi-or tri-cyclic spiro or fused heterocyclic ring system;

R³ is H, R, NH₂ or NRR';

R⁴ is H, CH₂OH, C (O) OH, C (O) OR, C (O) NH₂, C (O) NHR or C (O) NRR’;

R⁵ is H, F, Cl or CF₃;

each of R⁶, R⁷ and R⁸ is independently H, F, CN, Cl, OH, NH₂, NRR’, R, OR, C (O) NH₂, C (O) NRR’, triazolyl, or P (O) RR’, provided that one of R⁶, R⁷ and R⁸ is P (O) RR’; and

each of R and R’ is independently a C_1-6 alkyl, cyclopropyl, cyclobutyl or cyclopentyl; or, R and R’ together with the N atom they are bonded to form a 5-membered heterocyclic ring,

or a pharmaceutically acceptable form or an isotope derivative thereof.
The compound of claim 1, wherein Ring A is a 6-membered heterocyclic ring, having the structural formula of (I^a) :

wherein

each of R¹ and R² is independently NH₂ or a C_1-6 alkyl, or R¹ and R², together with the carbon atom they are bound to, form a substituted or unsubstituted 5-membered carbocyclic or heterocyclic ring;

R³ is H, CH₃ or NH₂;

R⁴ is H, CH₂OH, C (O) OCH₃, C (O) NH₂, or C (O) NHCH₃;

R⁵ is H, Cl or CF₃;

each of R⁶, R⁷ and R⁸ is independently H, F, CN, Cl, OH, NH₂, CH₃, OCH₃, C (O) NH₂, 1, 2, 3-triazolyl, or P (O) RR’; and

each of R and R’ is independently a C_1-6 alkyl, cyclopropyl, cyclobutyl, or cyclopentyl.
The compound of claim 1 or 2, wherein Y¹ is CR⁷ and Y² is CR⁸, having the structural formula (II) :
The compound of claim 1 or 2, wherein Y¹ is N and Y² is CR⁸, having the structural formula (III) :
The compound of claim 1 or 2, wherein Y¹ is CR⁷ and Y² is N, having the structural formula (IV) :
The compound of claim 1 or 2, wherein X is S, having the structural formula (V) :
The compound of claim 6, wherein Y¹ is N, having the structural formula (VI) :
The compound of claim 6, wherein Y² is N, having the structural formula (VII) :
The compound of claim 6, wherein Y¹ is CR⁷ and Y² is CR⁸, having the structural formula (VIII) :
The compound of claim 1 or 2, wherein X is a single bond, having the structural formula (IX) :
The compound of claim 10, wherein Y¹ is N and Y² is CR⁸, having the structural formula (X) :
The compound of claim 10, wherein Y¹ is CR⁷ and Y² is N, having the structural formula (XI) :
The compound of claim 10, wherein Y¹ is CR⁷ and Y² is CR⁸, having the structural formula (XII) :
The compound of claim 1 or 2, wherein R¹ and R² form a 5-membered carbocyclic or heterocyclic ring, optionally substituted, having the formula (XIII) :

wherein

Z is O or CH₂,

R⁹ is independently CH₃ or NH₂, and

i is 0, 1 or 2.
The compound of claim 6, wherein R¹ and R² form a 5-membered carbocyclic or heterocyclic ring, optionally substituted, having the formula (XIV) :

wherein

Z is O or CH₂,

R⁹ is independently CH₃ or NH₂, and

i is 0, 1 or 2.
The compound of claim 10, wherein R¹ and R² form a 5-membered carbocyclic or heterocyclic ring, optionally substituted, having the formula (XV) :

wherein

Z is O or CH₂,

R⁹ is independently CH₃ or NH₂, and

i is 0, 1 or 2.
The compound of any one of claims 14-16, wherein Z is O.
The compound of claim 17, wherein i is 2, and two R⁹’s are NH₂ and CH₃.
The compound of any one of claims 1-18, wherein R³ is NH₂.
The compound of any one of claims 1-18, wherein R³ is H.
The compound of any one of claims 1-18, wherein R³ is CH₃.
The compound of any one of claims 1-21, wherein R⁴ is H.
The compound of any one of claims 1-21, wherein R⁴ is CH₂OH.
The compound of any one of claims 1-21, wherein R⁴ is C (O) OCH₃.
The compound of any one of claims 1-21, wherein R⁴ is C (O) NH₂.
The compound of any one of claims 1-21, wherein R⁴ is C (O) NHCH₃.
The compound of any one of claims 1-26, wherein R⁵ is Cl.
The compound of any one of claims 1-26, wherein R⁵ is H.
The compound of any one of claims 1-26, wherein R⁵ is CF₃.
The compound of any one of claims 1-29, wherein R⁶ is Cl.
The compound of any one of claims 1-30, wherein one of R⁷ and R⁸ is P (O) RR’.
The compound of claim 31, wherein one or both of R and R’ is CH₃.
The compound of claim 31, wherein one or both of R and R’ is cyclopropyl.
The compound of claim 31, wherein one or both of R and R’ is cyclobutyl.
The compound of claim 31, wherein one or both of R and R’ is cyclopentyl.
A compound having the structural formula:

or a pharmaceutically acceptable form or an isotope derivative thereof.
A pharmaceutical composition comprising a compound according to any of claims 1-36 and a pharmaceutically acceptable excipient, carrier, or diluent.
The pharmaceutical composition of claim 37, effective to treat or reduce cancer, or a related disease or condition.
A unit dosage form comprising a pharmaceutical composition according to claim 37 or 38.
A method for treating or reducing a disease or condition, comprising administering to a subject in need thereof a therapeutically effective amount of a compound according to any of claims 1-36 and a pharmaceutically acceptable excipient, carrier, or diluent.
The method of claim 40, wherein the disease or condition is mediated by the activity of SHP2.
The method of claim 40, wherein the disease or condition is selected from Noonan syndrome, leopard syndrome, juvenile myelomonocytic leukemias, liver cancer, neuroblastoma, melanoma, squamous-cell carcinoma of the head and neck, acute myeloid leukemia, breast cancer, esophageal cancer, lung cancer, colon cancer, head cancer, gastric carcinoma, neuroblastoma, anaplastic large-cell lymphoma and glioblastoma
The method of any one of claims 40-42, wherein the disease or condition is cancer, or a related disease or condition thereof.
The method of any one of claims 40-43, further comprising administering to the subject one or more of chemotherapy, immunotherapy, radiotherapy, targeted therapy and hormonal therapy.
The method of claim 44, comprising administering an immunotherapy.
The method of claim 44, comprising administering a chemotherapy agent.
The method of claim 44, comprising administering a radiotherapy.
Use of a compound of any of claims 1-36 for treating or reducing a disease or condition mediated by the activity of SHP2.
Use of a compound of any of claims 1-36, and a pharmaceutically acceptable excipient, carrier, or diluent, in preparation of a medicament for treating or reducing a disease or condition.
Use of claims 48 or 49, wherein the disease or condition is cancer, or a related disease or condition thereof.