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WO2023249393A1 - Composition for prevention, amelioration or treatment of coronavirus infection comprising medicinal herb extract as active ingredient - Google Patents

Composition for prevention, amelioration or treatment of coronavirus infection comprising medicinal herb extract as active ingredient Download PDF

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Publication number
WO2023249393A1
WO2023249393A1 PCT/KR2023/008574 KR2023008574W WO2023249393A1 WO 2023249393 A1 WO2023249393 A1 WO 2023249393A1 KR 2023008574 W KR2023008574 W KR 2023008574W WO 2023249393 A1 WO2023249393 A1 WO 2023249393A1
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WIPO (PCT)
Prior art keywords
composition
extract
coronavirus
active ingredient
preventing
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PCT/KR2023/008574
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French (fr)
Korean (ko)
Inventor
권선오
진영희
김상현
윤지원
이상명
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한국 한의학 연구원
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Publication of WO2023249393A1 publication Critical patent/WO2023249393A1/en

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin

Definitions

  • the present invention relates to a composition for preventing, improving, or treating coronavirus infection containing herbal extracts, especially rare extracts, as active ingredients.
  • the new type 2 severe acute respiratory syndrome coronavirus (SARS-CoV2) is a virus that has a single-stranded positive RNA genome consisting of approximately 30,000 base pairs, and the viral surface is surrounded by an envelope. Since it was first reported in Wuhan, China at the end of December 2019, the number of infected people has rapidly increased worldwide. The virus spreads so efficiently between people that the World Health Organization has declared it a pandemic with the potential to spread around the world and is seeking responses.
  • Typical Covid-19 patients show relatively mild symptoms such as increased body temperature, cough, and chest pain, but some patients develop serious diseases such as difficulty breathing and pneumonia, leading to death.
  • coronavirus infections include ribavirin, famciclovir, nitazoxanide, nafamostat, and chloroquine.
  • Remdesi Remdesivir (GS-5734) and favipiravir are being used to treat coronavirus infections, but their effectiveness is low and the incidence of variant viruses is high due to the nature of nucleic acid analog antivirals, so two or more types of antivirals are applied together. .
  • Most antiviral drugs used for coronavirus infections have the ability to inhibit RNA polymerase, but mouse hepatitis virus, a type of coronavirus that is resistant to remdesivir, has been discovered, and existing antiviral drugs have been discovered. Viral drugs are ineffective.
  • antiviral drugs developed to date have severe side effects, so great caution is required in their application.
  • Korean Patent Publication No. 2022-0013625 discloses an anti-coronavirus composition characterized by containing an extract of Ulgoebul tree as an active ingredient
  • Korean Patent Publication No. 2022-0018953 discloses an anti-coronavirus composition containing fern extract or a fraction thereof.
  • the present invention was developed in response to the above-mentioned needs, by providing an antiviral composition for coronavirus containing a rare extract as an active ingredient, and confirming that the rare extract inhibits coronavirus infection and proliferation.
  • the invention was completed.
  • the present invention provides an antiviral composition for coronavirus containing Siegesbeckia glabrescens extract as an active ingredient.
  • the present invention provides a pharmaceutical composition for preventing or treating coronavirus infection, comprising Siegesbeckia glabrescens extract as an active ingredient.
  • the present invention provides a health functional food composition for preventing or improving coronavirus infection containing Siegesbeckia glabrescens extract as an active ingredient.
  • the present invention provides a quasi-drug composition for preventing or improving coronavirus infection containing Siegesbeckia glabrescens extract as an active ingredient.
  • the present invention provides a cosmetic composition for preventing or improving coronavirus infection containing Siegesbeckia glabrescens extract as an active ingredient.
  • the present invention provides a feed additive for preventing or improving coronavirus infection containing Siegesbeckia glabrescens extract as an active ingredient.
  • the present invention relates to a composition for preventing, ameliorating or treating coronavirus infection containing an extract of Heereum as an active ingredient.
  • the extract of Heereum has the effect of protecting cells from coronavirus infection and inhibits the replication of the virus within cells and the inhibition of the virus in cells. It has the effect of suppressing my infection.
  • Figure 1 shows the results of confirming the antiviral effect of Heereum extract against SARS-CoV2 through CPE (Cytopathic effect) inhibition analysis.
  • A is a photo of a plate stained with crystal violet showing the antiviral effect against SARS-CoV2 according to treatment with Heeryeom extract at different concentrations, and
  • B is the result of quantifying this.
  • NC is the normal group
  • VC is the control group treated with SARS-CoV2.
  • Figure 2 shows the results confirming the viral replication inhibitory effect of Heereum extract on SARS-CoV2.
  • NC is the normal group
  • VC is the control group treated with SARS-CoV2.
  • **** means that there was a statistically significant decrease in the number of viral copies in the group treated with SARS-CoV2 and rare extract compared to the control group (VC) treated with SARS-CoV2, p ⁇ 0.0001.
  • Figure 3 is a fluorescent photograph (A) confirming the viral infection inhibition effect of Heereum extract on SARS-CoV2 and the quantification result (B).
  • NC is the normal group
  • VC is the control group treated with SARS-CoV2. **** means that there was a statistically significant decrease in viral infection in the group treated with SARS-CoV2 and rare extract compared to the control group (VC) treated with SARS-CoV2, p ⁇ 0.0001.
  • the present invention provides an antiviral composition against coronavirus containing Siegesbeckia glabrescens extract as an active ingredient.
  • the coronavirus may be one or more types selected from the group consisting of severe acute respiratory syndrome coronavirus type 2 (SARS-CoV2), severe acute respiratory syndrome coronavirus (SARS CoV), and Middle East respiratory syndrome (MERS) coronavirus, Preferably, it is type 2 severe acute respiratory syndrome coronavirus (SARS-CoV2), but is not limited thereto.
  • SARS-CoV2 severe acute respiratory syndrome coronavirus
  • SARS CoV severe acute respiratory syndrome coronavirus
  • SARS CoV severe acute respiratory syndrome coronavirus
  • MERS Middle East respiratory syndrome
  • the extraction solvent for the rare extract is preferably water, a lower alcohol of C 1 to C 4 , or a mixture thereof, and more preferably water, but is not limited thereto.
  • any part of the above-mentioned heiryeom can be used, and preferably the aerial part is used, but it is not limited to this.
  • the Heereum extract of the present invention has the effect of inhibiting the proliferation of type 2 severe acute respiratory syndrome coronavirus.
  • the present invention provides a pharmaceutical composition for preventing or treating coronavirus infection, comprising Siegesbeckia glabrescens extract as an active ingredient.
  • the pharmaceutical composition according to the present invention can be formulated and used in the form of oral dosage forms such as capsules, powders, granules, tablets, suspensions, emulsions, syrups, aerosols, external preparations, suppositories, and sterile injection solutions according to conventional methods. You can.
  • the pharmaceutical composition according to the present invention may further include a pharmaceutically acceptable carrier, excipient, or diluent.
  • Carriers, excipients and diluents that may be included in the pharmaceutical composition of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate. , cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oil.
  • Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc. These solid preparations contain at least one excipient such as starch, calcium carbonate, sucrose or lactose, gelatin, etc. in the pharmaceutical composition. It is prepared by mixing. Additionally, in addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral use include suspensions, oral solutions, emulsions, syrups, etc.
  • Preparations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories.
  • Non-aqueous solvents and suspensions include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate.
  • injectable ester such as ethyl oleate.
  • Withepsol, Macrogol, Tween 61, cacao, laurin, glycerogelatin, etc. can be used as a base for suppositories.
  • the appropriate dosage of the pharmaceutical composition of the present invention may be prescribed in various ways depending on factors such as formulation method, administration method, patient's age, weight, sex, pathological condition, food, administration time, administration route, excretion rate, and reaction sensitivity. You can.
  • the pharmaceutical composition of the present invention can be administered orally or parenterally, and in the case of parenteral administration, it can be administered topically on the skin, intravenous injection, subcutaneous injection, intramuscular injection, intraperitoneal injection, transdermal administration, etc.
  • the present invention provides a health functional food composition for preventing or improving coronavirus infection containing Siegesbeckia glabrescens extract as an active ingredient.
  • composition may be manufactured in any one formulation selected from powder, granule, pill, tablet, capsule, candy, syrup, and beverage, but is not limited thereto.
  • the health functional food composition of the present invention When using the health functional food composition of the present invention as a food additive, the health functional food composition may be added as is or used together with other foods or food ingredients, and may be used appropriately according to conventional methods.
  • the amount of active ingredient can be appropriately used depending on the purpose of use (prevention or improvement).
  • the health functional food composition of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less, based on the total raw materials.
  • the amount may be below the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount above the above range.
  • Health functional foods There are no particular restrictions on the types of health functional foods. Examples of foods to which the health functional food composition can be added include meat, sausages, bread, chocolate, candies, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, and tea. There are drinks, alcoholic beverages, and vitamin complexes, and it includes all health foods in the conventional sense.
  • the health functional food composition of the present invention can be manufactured into food, especially functional food.
  • the functional food of the present invention includes ingredients commonly added during food production, and includes, for example, proteins, carbohydrates, fats, nutrients and seasonings.
  • ingredients commonly added during food production includes, for example, proteins, carbohydrates, fats, nutrients and seasonings.
  • natural carbohydrates or flavoring agents may be included as additional ingredients in addition to the active ingredient.
  • the natural carbohydrates include monosaccharides (e.g., glucose, fructose, etc.), disaccharides (e.g., maltose, sucrose, etc.), oligosaccharides, polysaccharides (e.g., dextrins, cyclodextrins, etc.), or sugar alcohols (e.g., For example, xylitol, sorbitol, erythritol, etc.) is preferable.
  • the flavoring agent may be a natural flavoring agent (e.g., thaumatin, stevia extract, etc.) or a synthetic flavoring agent (e.g., saccharin, aspartame, etc.).
  • the ratio of the ingredients added is not very important, but is generally selected in the range of 0.01 to 0.1 parts by weight based on 100 parts by weight of the health functional food composition of the present invention.
  • the present invention provides a quasi-drug composition for preventing or improving coronavirus infection containing Siegesbeckia glabrescens extract as an active ingredient.
  • the quasi-drugs may include external skin preparations and personal hygiene products.
  • it may be a disinfectant cleaner, shower foam, garnish, wet tissue, detergent soap, hand wash, or ointment, but is not limited thereto.
  • the composition can be added as is or used with other quasi-drugs or quasi-drug components, and can be used appropriately according to conventional methods.
  • the mixing amount of the active ingredient can be appropriately determined depending on the purpose of use.
  • the quasi-drug composition of the present invention may be manufactured in the form of a general emulsified formulation or solubilized formulation.
  • it may have a formulation such as lotion, cream, ointment, spray, oil gel, gel, oil, or aerosol, but can be used without limitation as long as it shows the effect of preventing or improving the coronavirus infection of the present invention. there is.
  • the quasi-drug composition contains oil, water, surfactants, moisturizers, lower alcohols with 1 to 4 carbon atoms, thickeners, chelating agents, colorants, preservatives or fragrances, etc., which are generally mixed in quasi-drug compositions, as appropriate for each dosage form. Can be used in combination.
  • the present invention provides a cosmetic composition for preventing or improving coronavirus infection containing Siegesbeckia glabrescens extract as an active ingredient.
  • the cosmetic composition of the present invention includes ingredients commonly used in cosmetic compositions, such as antioxidants, stabilizers, solubilizers, vitamins, conventional auxiliaries such as pigments and fragrances, and carriers.
  • the cosmetic composition of the present invention can be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing products. , oil, powder foundation, emulsion foundation, wax foundation, spray, etc., but is not limited thereto. More specifically, it can be manufactured in the form of flexible lotion (skin), nourishing lotion (milk lotion), nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, shampoo, pack, spray or powder. You can.
  • the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tragacantha, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide are used as carrier ingredients. It can be.
  • the formulation of the present invention is a powder or spray
  • lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder can be used as the carrier ingredient.
  • chlorofluorohydrocarbon and propane may be used as carrier ingredients.
  • May contain propellants such as butane or dimethyl ether.
  • a solvent, solubilizing agent, or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 , 3-butyl glycol oil, glycerol aliphatic esters, polyethylene glycol or fatty acid esters of sorbitan.
  • the carrier ingredients include water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, and microcrystals.
  • a liquid diluent such as ethanol or propylene glycol
  • a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester
  • microcrystals ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester
  • Cellulose, aluminum metahydroxide, bentonite, agar or tragacantha can be used.
  • the carrier ingredients include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, and fatty acid amide.
  • Ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester can be used.
  • the present invention provides a feed additive for preventing or improving coronavirus infection containing Siegesbeckia glabrescens extract as an active ingredient.
  • the feed additive of the present invention corresponds to supplementary feed under the Feed Management Act.
  • the term 'feed' may mean any natural or artificial diet, meal, etc., or a component of the meal, for or suitable for eating, ingestion, and digestion by animals.
  • the type of feed is not particularly limited, and feed commonly used in the art can be used.
  • Non-limiting examples of the feed include plant feeds such as grains, roots and fruits, food processing by-products, algae, fiber, pharmaceutical by-products, oils and fats, starches, cucurbits or grain by-products;
  • animal feeds such as proteins, inorganic substances, fats and oils, minerals, oils and fats, single-cell proteins, zooplanktons or food. These may be used alone or in combination of two or more types.
  • Heeryum purchased a standard product from Gwangmyeongdang Pharmaceutical. Specifically, 10 parts by weight of water (100°C) was added to 1 part by weight of Heeryum dried raw material, extracted under reflux for 3 hours, and then filtered through a strainer (extraction cloth). The material passed through a 53 ⁇ m filter was concentrated under reduced pressure and then freeze-dried to prepare a rare extract.
  • SARS-CoV2 was inoculated at an m.o.i of 0.01.
  • the rare extract of Preparation Example 1 was diluted with DMEM containing 2% (v/v) FBS and 1% (v/v) penicillin-streptomycin and cultured for 3 days.
  • the cell culture medium was removed, and then treated with a 1:1 volume ratio of methanol:acetone and fixed for 10 minutes at room temperature.
  • the solution was removed, 0.4% (w/v) crystal violet was added to each well, and the cells were stained at room temperature for 10 minutes, then all of the solution was removed and dried.
  • methanol:acetic acid was mixed at a 1:1 volume ratio, and 100 ⁇ l of a solution diluted 5 times with distilled water was added to each well. Crystal violet was extracted for 2 hours, and the absorbance was measured at 550 nm.
  • the Heereum extract of the present invention inhibited the proliferation of SARS-CoV2, and the IC 50 value was confirmed to be 90.36 ⁇ g/mL, indicating that the Heereum extract is an antibody that protects cells from the SARS-CoV2 virus. The virus effect was excellent.
  • the viral replication inhibitory effect of Heereum extract was confirmed by qPCR. Specifically, after culturing Vero E6 cells in a 96-well plate, SARS-CoV2 was inoculated at an m.o.i of 0.01, samples of Preparation Example 1 were treated at different concentrations, and cultured for 24 hours. After incubation, viral RNA released out of the cells was extracted using Bioneer's AccuPrep Viral RNA Extraction kit. Then, cDNA was synthesized using Toyobo's ReverTra Ace qPCR RT kit, then PCR was performed with specific primers for the RBD of the spike protein, and the viral RNA copy number was measured using the spike protein gene plasmid standard sample. Calculated.
  • the extract of Heereum was excellent in inhibiting the replication of the SARS-CoV2 virus.
  • the inhibitory effect of SARS-CoV2 virus infection using Heereum extract was confirmed using immunofluorescence analysis. Specifically, Vero E6 cells were distributed on a plate at 1 ⁇ 10 4 cells/well, and samples of Preparation Example 1 were processed at each concentration, followed by 2% (v/v) FBS and 1% (v/v) antibiotics. SARS-CoV2 diluted 1000 times was treated with DMEM and cultured for 24 hours. After incubation, the cells were fixed with a 4% (v/v) formaldehyde solution, reacted with a primary antibody (Nucleocapsid protein) diluted 2000 times, and then reacted with a secondary antibody diluted 200 times. After the reaction, the cells were observed under a fluorescence microscope, and the fluorescence intensity was analyzed.

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Abstract

The present invention relates to a composition for the prevention, amelioration or treatment of coronavirus infection, the composition comprising a Siegesbeckia pubescens extract as an active ingredient. The Siegesbeckia pubescens extract has the effect of protecting cells from coronavirus infection, and has an excellent effect of suppressing viral replication in cells and viral infection in cells, and thus, the composition comprising the Siegesbeckia pubescens extract as an active ingredient according to the present invention can be effectively used as a therapeutic agent for coronavirus infection or as an anti-coronavirus health functional food, quasi-drug, cosmetic, or feed additive.

Description

한약재 추출물을 유효성분으로 포함하는 코로나바이러스 감염증의 예방, 개선 또는 치료용 조성물Composition for preventing, improving or treating coronavirus infection containing herbal medicine extract as an active ingredient
본 발명은 한약재 추출물, 특히 희렴 추출물을 유효성분으로 포함하는 코로나바이러스 감염증의 예방, 개선 또는 치료용 조성물에 관한 것이다.The present invention relates to a composition for preventing, improving, or treating coronavirus infection containing herbal extracts, especially rare extracts, as active ingredients.
본 결과물은 과학기술정보통신부의 한국한의학연구원연구운영비지원(R＆D) 사업의 지원을 받아 수행되었습니다(과제번호: 1711175247).This work was carried out with support from the Korea Institute of Oriental Medicine Research and Operational Expenses (R&D) Project of the Ministry of Science and ICT (Project Number: 1711175247).
신종 제2형 중증급성호흡기증후군 코로나바이러스(SARS-CoV2)는 약 3만 개의 염기쌍으로 이루어진 단일 가닥의 양성 RNA 게놈을 가지고 있고, 바이러스 표면이 외피로 둘러싸여 있는 바이러스이다. 2019년 12월 말에 중국 우한에서 최초로 보고된 후 전 세계적으로 감염자가 폭증하였다. 상기 바이러스는 사람 사이의 전파가 매우 효율적으로 이루어져 세계보건기구에서도 전 세계에 유행이 가능한 펜데믹으로 선포하고 대응을 모색하고 있다.The new type 2 severe acute respiratory syndrome coronavirus (SARS-CoV2) is a virus that has a single-stranded positive RNA genome consisting of approximately 30,000 base pairs, and the viral surface is surrounded by an envelope. Since it was first reported in Wuhan, China at the end of December 2019, the number of infected people has rapidly increased worldwide. The virus spreads so efficiently between people that the World Health Organization has declared it a pandemic with the potential to spread around the world and is seeking responses.
일반적인 코비드-19 환자는 체온상승, 기침 및 가슴 통증과 같은 비교적 심하지 않은 증상을 보이지만, 일부 환자에게서는 호흡곤란 및 폐렴과 같은 심각한 질환으로 발전되어 사망에 이르게 된다. Typical Covid-19 patients show relatively mild symptoms such as increased body temperature, cough, and chest pain, but some patients develop serious diseases such as difficulty breathing and pneumonia, leading to death.
현재까지 코로나바이러스 감염증에 대해 공인된 치료제로는 리바비린(ribavirin), 팜시클로비르(famciclovir), 니타족사나이드(nitazoxanide), 나파모스타트(nafamostat) 및 클로로퀸(chloroquine) 등이 알려져 있으며, 이외에도 렘데시비르(remdesivir, GS-5734)와 파비피라비르(favipiravir)가 코로나바이러스 감염증 치료에 사용되고 있으나, 효과가 낮고 핵산 아날로그형 항바이러스제들의 특성상 변종바이러스의 발생율이 높아 2종 이상의 항바이러스제를 같이 적용하고 있다. 코로나바이러스 감염증에 사용되는 항바이러스제들은 대부분 RNA 폴리머라아제(polymerase) 억제능을 가지고 있지만, 렘데시비르에 대한 내성을 가지는 코로나바이러스의 일종인 마우스 간염 바이러스(Mouse hepatitis virus)가 발견되었으며, 기존의 항바이러스제의 효과가 없는 실정이다. 또한, 현재까지 개발된 항바이러스제들은 심한 부작용을 나타내고 있으므로, 그 응용에 있어서 많은 주의가 필요하다. To date, recognized treatments for coronavirus infections include ribavirin, famciclovir, nitazoxanide, nafamostat, and chloroquine. In addition, Remdesi Remdesivir (GS-5734) and favipiravir are being used to treat coronavirus infections, but their effectiveness is low and the incidence of variant viruses is high due to the nature of nucleic acid analog antivirals, so two or more types of antivirals are applied together. . Most antiviral drugs used for coronavirus infections have the ability to inhibit RNA polymerase, but mouse hepatitis virus, a type of coronavirus that is resistant to remdesivir, has been discovered, and existing antiviral drugs have been discovered. Viral drugs are ineffective. In addition, antiviral drugs developed to date have severe side effects, so great caution is required in their application.
또한, 현재 가장 일반적인 치료 방법은 증상을 완화하는 대증요법이다. 따라서 코로나바이러스의 발생을 예방하고 치료하기 위한 감염 억제 효과가 뛰어나고 독성이 적은 새로운 항코로나바이러스제의 개발 필요성이 증가되고 있다.Additionally, currently the most common treatment method is symptomatic therapy to relieve symptoms. Therefore, the need to develop new anti-coronavirus drugs with excellent infection control effects and low toxicity to prevent and treat coronavirus outbreaks is increasing.
한편, 한국공개특허 제2022-0013625호에는 울괴불나무 추출물을 유효성분으로 포함하는 것을 특징으로 하는 항 코로나바이러스 조성물이 개시되어 있고, 한국공개특허 제2022-0018953호에는 고사리 추출물 또는 이의 분획물을 포함하는 항바이러스용 조성물이 개시되어 있지만, 본 발명의 한약재 추출물, 특히 희렴 추출물을 유효성분으로 포함하는 코로나바이러스 감염증의 예방, 개선 또는 치료용 조성물에 관해 전혀 개시된 바 없다. Meanwhile, Korean Patent Publication No. 2022-0013625 discloses an anti-coronavirus composition characterized by containing an extract of Ulgoebul tree as an active ingredient, and Korean Patent Publication No. 2022-0018953 discloses an anti-coronavirus composition containing fern extract or a fraction thereof. Although an antiviral composition has been disclosed, there has been no disclosure at all regarding a composition for preventing, improving, or treating coronavirus infection containing the herbal medicine extract of the present invention, especially the rare extract, as an active ingredient.
본 발명은 상기와 같은 요구에 의해 도출된 것으로, 희렴 추출물을 유효성분으로 포함하는 코로나바이러스에 대한 항바이러스용 조성물을 제공하고, 상기 희렴 추출물이 코로나바이러스 감염 및 증식을 억제하는 것을 확인함으로써, 본 발명을 완성하였다.The present invention was developed in response to the above-mentioned needs, by providing an antiviral composition for coronavirus containing a rare extract as an active ingredient, and confirming that the rare extract inhibits coronavirus infection and proliferation. The invention was completed.
상기 과제를 해결하기 위하여, 본 발명은 희렴(Siegesbeckia glabrescens) 추출물을 유효성분으로 포함하는 코로나바이러스에 대한 항바이러스용 조성물을 제공한다. In order to solve the above problems, the present invention provides an antiviral composition for coronavirus containing Siegesbeckia glabrescens extract as an active ingredient.
또한, 본 발명은 희렴(Siegesbeckia glabrescens) 추출물을 유효성분으로 포함하는 코로나바이러스 감염증의 예방 또는 치료용 약학 조성물을 제공한다. In addition, the present invention provides a pharmaceutical composition for preventing or treating coronavirus infection, comprising Siegesbeckia glabrescens extract as an active ingredient.
또한, 본 발명은 희렴(Siegesbeckia glabrescens) 추출물을 유효성분으로 포함하는 코로나바이러스 감염증의 예방 또는 개선용 건강기능식품 조성물을 제공한다. In addition, the present invention provides a health functional food composition for preventing or improving coronavirus infection containing Siegesbeckia glabrescens extract as an active ingredient.
또한, 본 발명은 희렴(Siegesbeckia glabrescens) 추출물을 유효성분으로 포함하는 코로나바이러스 감염증의 예방 또는 개선용 의약외품 조성물을 제공한다. In addition, the present invention provides a quasi-drug composition for preventing or improving coronavirus infection containing Siegesbeckia glabrescens extract as an active ingredient.
또한, 본 발명은 희렴(Siegesbeckia glabrescens) 추출물을 유효성분으로 포함하는 코로나바이러스 감염증의 예방 또는 개선용 화장료 조성물을 제공한다. In addition, the present invention provides a cosmetic composition for preventing or improving coronavirus infection containing Siegesbeckia glabrescens extract as an active ingredient.
또한, 본 발명은 희렴(Siegesbeckia glabrescens) 추출물을 유효성분으로 포함하는 코로나바이러스 감염증의 예방 또는 개선용 사료 첨가제를 제공한다.In addition, the present invention provides a feed additive for preventing or improving coronavirus infection containing Siegesbeckia glabrescens extract as an active ingredient.
본 발명은 희렴 추출물을 유효성분으로 포함하는 코로나바이러스 감염증의 예방, 개선 또는 치료용 조성물에 관한 것으로, 희렴 추출물은 코로나바이러스 감염으로부터 세포를 보호하는 효과가 있고, 세포 내 바이러스의 복제 및 바이러스의 세포 내 감염을 억제하는 효과가 있다.The present invention relates to a composition for preventing, ameliorating or treating coronavirus infection containing an extract of Heereum as an active ingredient. The extract of Heereum has the effect of protecting cells from coronavirus infection and inhibits the replication of the virus within cells and the inhibition of the virus in cells. It has the effect of suppressing my infection.
도 1은 CPE(Cytopathic effect) 억제 분석으로 희렴 추출물의 SARS-CoV2에 대한 항바이러스 효과를 확인한 결과이다. (A)는 농도별 희렴 추출물 처리에 따른 SARS-CoV2에 대한 항바이러스 효과를 크리스탈 바이올렛으로 염색한 플레이트 사진이고, (B)는 이를 정량한 결과이다. NC는 정상군이고, VC는 SARS-CoV2를 처리한 대조군이다. Figure 1 shows the results of confirming the antiviral effect of Heereum extract against SARS-CoV2 through CPE (Cytopathic effect) inhibition analysis. (A) is a photo of a plate stained with crystal violet showing the antiviral effect against SARS-CoV2 according to treatment with Heeryeom extract at different concentrations, and (B) is the result of quantifying this. NC is the normal group, and VC is the control group treated with SARS-CoV2.
도 2는 희렴 추출물의 SARS-CoV2에 대한 바이러스 복제 억제 효과를 확인한 결과이다. NC는 정상군이고, VC는 SARS-CoV2를 처리한 대조군이다. ****은 SARS-CoV2를 처리한 대조군(VC) 대비 SARS-CoV2 및 희렴 추출물 처리군의 바이러스 복제 수가 통계적으로 유의미하게 감소하였다는 것을 의미하며, p<0.0001이다. Figure 2 shows the results confirming the viral replication inhibitory effect of Heereum extract on SARS-CoV2. NC is the normal group, and VC is the control group treated with SARS-CoV2. **** means that there was a statistically significant decrease in the number of viral copies in the group treated with SARS-CoV2 and rare extract compared to the control group (VC) treated with SARS-CoV2, p<0.0001.
도 3은 희렴 추출물의 SARS-CoV2에 대한 바이러스 감염 억제 효과를 확인한 형광사진(A) 및 이를 정량한 결과(B)이다. NC는 정상군이고, VC는 SARS-CoV2를 처리한 대조군이다. ****은 SARS-CoV2를 처리한 대조군(VC) 대비 SARS-CoV2 및 희렴 추출물 처리군의 바이러스 감염이 통계적으로 유의미하게 감소하였다는 것을 의미하며, p<0.0001이다.Figure 3 is a fluorescent photograph (A) confirming the viral infection inhibition effect of Heereum extract on SARS-CoV2 and the quantification result (B). NC is the normal group, and VC is the control group treated with SARS-CoV2. **** means that there was a statistically significant decrease in viral infection in the group treated with SARS-CoV2 and rare extract compared to the control group (VC) treated with SARS-CoV2, p<0.0001.
본 발명의 목적을 달성하기 위하여, 본 발명은 희렴(Siegesbeckia glabrescens) 추출물을 유효성분으로 포함하는 코로나바이러스에 대한 항바이러스용 조성물을 제공한다. In order to achieve the object of the present invention, the present invention provides an antiviral composition against coronavirus containing Siegesbeckia glabrescens extract as an active ingredient.
상기 코로나바이러스는 제2형 중증급성호흡기증후군 코로나바이러스(SARS-CoV2), 중증급성호흡기증후군 코로나바이러스(SARS CoV) 및 중동 호흡기 증후군(MERS) 코로나바이러스로 이루어진 군에서 선택된 1종 이상인 것일 수 있고, 바람직하게는 제2형 중증급성호흡기증후군 코로나바이러스(SARS-CoV2)인 것이지만, 이에 한정하는 것은 아니다. The coronavirus may be one or more types selected from the group consisting of severe acute respiratory syndrome coronavirus type 2 (SARS-CoV2), severe acute respiratory syndrome coronavirus (SARS CoV), and Middle East respiratory syndrome (MERS) coronavirus, Preferably, it is type 2 severe acute respiratory syndrome coronavirus (SARS-CoV2), but is not limited thereto.
상기 희렴 추출물의 추출용매는 물, C1~C4의 저급 알코올 또는 이들의 혼합물이 바람직하고, 더 바람직하게는 물이지만, 이에 한정하는 것은 아니다.The extraction solvent for the rare extract is preferably water, a lower alcohol of C 1 to C 4 , or a mixture thereof, and more preferably water, but is not limited thereto.
상기 희렴은 희렴의 어느 부위라도 사용할 수 있고, 바람직하게는 지상부를 사용한 것이지만, 이에 제한되지 않는다.Any part of the above-mentioned heiryeom can be used, and preferably the aerial part is used, but it is not limited to this.
본 발명의 희렴 추출물은 제2형 중증급성호흡기증후군 코로나바이러스의 증식을 억제하는 효과가 있다. The Heereum extract of the present invention has the effect of inhibiting the proliferation of type 2 severe acute respiratory syndrome coronavirus.
또한, 본 발명은 희렴(Siegesbeckia glabrescens) 추출물을 유효성분으로 포함하는 코로나바이러스 감염증의 예방 또는 치료용 약학 조성물을 제공한다. In addition, the present invention provides a pharmaceutical composition for preventing or treating coronavirus infection, comprising Siegesbeckia glabrescens extract as an active ingredient.
본 발명에 따른 상기 약학 조성물은 각각 통상의 방법에 따라 캡슐제, 산제, 과립제, 정제, 현탁액, 에멀젼, 시럽, 에어로졸 등의 경구형 제형, 외용제, 좌제 및 멸균 주사용액의 형태로 제형화하여 사용될 수 있다. The pharmaceutical composition according to the present invention can be formulated and used in the form of oral dosage forms such as capsules, powders, granules, tablets, suspensions, emulsions, syrups, aerosols, external preparations, suppositories, and sterile injection solutions according to conventional methods. You can.
본 발명에 따른 상기 약학 조성물은 약학적으로 허용 가능한 담체, 부형제 또는 희석제를 더 포함할 수 있다. The pharmaceutical composition according to the present invention may further include a pharmaceutically acceptable carrier, excipient, or diluent.
본 발명의 약학 조성물에 포함될 수 있는 담체, 부형제 및 희석제로는 락토즈, 덱스트로즈, 수크로스, 솔비톨, 만니톨, 자일리톨, 에리스리톨, 말티톨, 전분, 아카시아 고무, 알지네이트, 젤라틴, 칼슘 포스페이트, 칼슘 실리케이트, 셀룰로오스, 메틸 셀룰로오스, 미정질 셀룰로오스, 폴리비닐 피롤리돈, 물, 메틸히드록시벤조에이트, 프로필히드록시벤조에이트, 탈크, 마그네슘 스테아레이트 및 광물유 등을 포함한 다양한 화합물 혹은 혼합물을 들 수 있다. Carriers, excipients and diluents that may be included in the pharmaceutical composition of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate. , cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oil.
제제화할 경우에는 보통 사용하는 충진제, 증량제, 결합제, 습윤제, 붕해제, 계면활성제 등의 희석제 또는 부형제를 사용하여 조제된다. 경구투여를 위한 고형제제에는 정제, 환제, 산제, 과립제, 캡슐제 등이 포함되며, 이러한 고형제제는 상기 약학 조성물에 적어도 하나 이상의 부형제 예를 들면, 전분, 칼슘카보네이트, 수크로스 또는 락토오스, 젤라틴 등을 섞어 조제된다. 또한, 단순한 부형제 이외에 마그네슘 스테아레이트, 탈크 같은 윤활제들도 사용된다. 경구를 위한 액상 제제로는 현탁제, 내용액제, 유제, 시럽제 등이 해당하는데 흔히 사용되는 단순 희석제인 물, 리퀴드 파라핀 이외에 여러 가지 부형제, 예를 들면 습윤제, 감미제, 방향제, 보존제 등이 포함될 수 있다. 비경구투여를 위한 제제에는 멸균된 수용액, 비수성용제, 현탁제, 유제, 동결건조 제제, 좌제가 포함된다. 비수성용제, 현탁제로는 프로필렌글리콜, 폴리에틸렌 글리콜, 올리브 오일과 같은 식물성 기름, 에틸올레이트와 같은 주사 가능한 에스테르 등이 사용될 수 있다. 좌제의 기제로는 위텝솔, 마크로골, 트윈 61, 카카오지, 라우린지, 글리세로젤라틴 등이 사용될 수 있다.When formulated, it is prepared using diluents or excipients such as commonly used fillers, extenders, binders, wetting agents, disintegrants, and surfactants. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc. These solid preparations contain at least one excipient such as starch, calcium carbonate, sucrose or lactose, gelatin, etc. in the pharmaceutical composition. It is prepared by mixing. Additionally, in addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral use include suspensions, oral solutions, emulsions, syrups, etc. In addition to the commonly used simple diluents such as water and liquid paraffin, various excipients such as wetting agents, sweeteners, fragrances, and preservatives may be included. . Preparations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Non-aqueous solvents and suspensions include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, and injectable ester such as ethyl oleate. As a base for suppositories, Withepsol, Macrogol, Tween 61, cacao, laurin, glycerogelatin, etc. can be used.
본 발명의 약학 조성물의 적합한 투여량은 제제화 방법, 투여 방식, 환자의 연령, 체중, 성, 병적 상태, 음식, 투여 시간, 투여 경로, 배설 속도 및 반응 감응성과 같은 요인들에 의해 다양하게 처방될 수 있다.The appropriate dosage of the pharmaceutical composition of the present invention may be prescribed in various ways depending on factors such as formulation method, administration method, patient's age, weight, sex, pathological condition, food, administration time, administration route, excretion rate, and reaction sensitivity. You can.
본 발명의 약학 조성물은 경구 또는 비경구로 투여할 수 있으며, 비경구투여의 경우, 피부에 국소적으로 도포, 정맥 내 주입, 피하 주입, 근육 주입, 복강 주입, 경피 투여 등으로 투여할 수 있다.The pharmaceutical composition of the present invention can be administered orally or parenterally, and in the case of parenteral administration, it can be administered topically on the skin, intravenous injection, subcutaneous injection, intramuscular injection, intraperitoneal injection, transdermal administration, etc.
또한, 본 발명은 희렴(Siegesbeckia glabrescens) 추출물을 유효성분으로 포함하는 코로나바이러스 감염증의 예방 또는 개선용 건강기능식품 조성물을 제공한다. In addition, the present invention provides a health functional food composition for preventing or improving coronavirus infection containing Siegesbeckia glabrescens extract as an active ingredient.
상기 조성물은 분말, 과립, 환, 정제, 캡슐, 캔디, 시럽 및 음료 중에서 선택된 어느 하나의 제형으로 제조될 수 있지만, 이에 한정하는 것은 아니다. The composition may be manufactured in any one formulation selected from powder, granule, pill, tablet, capsule, candy, syrup, and beverage, but is not limited thereto.
본 발명의 건강기능식품 조성물을 식품첨가물로 사용하는 경우, 상기 건강기능식품 조성물을 그대로 첨가하거나 다른 식품 또는 식품성분과 함께 사용될 수 있고, 통상적인 방법에 따라 적절하게 사용될 수 있다. 유효성분의 양은 그의 사용 목적(예방 또는 개선)에 따라 적절하게 사용될 수 있다. 일반적으로, 식품 또는 음료의 제조시 본 발명의 건강기능식품 조성물은 총 원료에 대하여 15 중량부 이하, 바람직하게는 10 중량부 이하의 양으로 첨가된다. 그러나 건강을 목적으로 하는 장기간의 섭취인 경우에는 상기 양은 상기 범위 이하일 수 있으며, 안전성 면에서 아무런 문제가 없기 때문에 유효성분은 상기 범위 이상의 양으로 사용될 수 있다.When using the health functional food composition of the present invention as a food additive, the health functional food composition may be added as is or used together with other foods or food ingredients, and may be used appropriately according to conventional methods. The amount of active ingredient can be appropriately used depending on the purpose of use (prevention or improvement). Generally, when manufacturing a food or beverage, the health functional food composition of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less, based on the total raw materials. However, in the case of long-term intake for health purposes, the amount may be below the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount above the above range.
상기 건강기능식품의 종류에 특별한 제한은 없다. 상기 건강기능식품 조성물을 첨가할 수 있는 식품의 예로는 육류, 소시지, 빵, 초콜릿, 캔디류, 스낵류, 과자류, 피자, 라면, 기타 면류, 껌류, 아이스크림류를 포함한 낙농제품, 각종 스프, 음료수, 차 드링크제, 알콜 음료 및 비타민 복합제 등이 있으며, 통상적인 의미에서의 건강식품을 모두 포함한다.There are no particular restrictions on the types of health functional foods. Examples of foods to which the health functional food composition can be added include meat, sausages, bread, chocolate, candies, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, and tea. There are drinks, alcoholic beverages, and vitamin complexes, and it includes all health foods in the conventional sense.
또한, 본 발명의 건강기능식품 조성물은 식품, 특히 기능성 식품으로 제조될 수 있다. 본 발명의 기능성 식품은 식품 제조 시에 통상적으로 첨가되는 성분을 포함하며, 예를 들어, 단백질, 탄수화물, 지방, 영양소 및 조미제를 포함한다. 예컨대, 드링크제로 제조되는 경우에는 유효성분 이외에 천연 탄수화물 또는 향미제를 추가 성분으로서 포함할 수 있다. 상기 천연 탄수화물은 모노사카라이드(예컨대, 글루코오스, 프럭토오스 등), 디사카라이드(예컨대, 말토스, 수크로스 등), 올리고당, 폴리사카라이드(예컨대, 덱스트린, 시클로덱스트린 등) 또는 당알코올(예컨대, 자일리톨, 소르비톨, 에리쓰리톨 등)인 것이 바람직하다. 상기 향미제는 천연 향미제(예컨대, 타우마틴, 스테비아 추출물 등)와 합성 향미제(예컨대, 사카린, 아스파르탐 등)를 이용할 수 있다.Additionally, the health functional food composition of the present invention can be manufactured into food, especially functional food. The functional food of the present invention includes ingredients commonly added during food production, and includes, for example, proteins, carbohydrates, fats, nutrients and seasonings. For example, when manufactured as a drink, natural carbohydrates or flavoring agents may be included as additional ingredients in addition to the active ingredient. The natural carbohydrates include monosaccharides (e.g., glucose, fructose, etc.), disaccharides (e.g., maltose, sucrose, etc.), oligosaccharides, polysaccharides (e.g., dextrins, cyclodextrins, etc.), or sugar alcohols (e.g., For example, xylitol, sorbitol, erythritol, etc.) is preferable. The flavoring agent may be a natural flavoring agent (e.g., thaumatin, stevia extract, etc.) or a synthetic flavoring agent (e.g., saccharin, aspartame, etc.).
상기 건강기능식품 조성물 이외에 여러 가지 영양제, 비타민, 전해질, 풍미제, 착색제, 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알코올, 탄산음료에 사용되는 탄산화제 등을 더 함유할 수 있다. 이러한 상기 첨가되는 성분의 비율은 크게 중요하진 않지만 본 발명의 건강기능식품 조성물 100 중량부에 대하여, 0.01 내지 0.1 중량부의 범위에서 선택되는 것이 일반적이다.In addition to the above health functional food composition, various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonic acid. It may further contain carbonating agents used in beverages. The ratio of the ingredients added is not very important, but is generally selected in the range of 0.01 to 0.1 parts by weight based on 100 parts by weight of the health functional food composition of the present invention.
또한, 본 발명은 희렴(Siegesbeckia glabrescens) 추출물을 유효성분으로 포함하는 코로나바이러스 감염증의 예방 또는 개선용 의약외품 조성물을 제공한다. In addition, the present invention provides a quasi-drug composition for preventing or improving coronavirus infection containing Siegesbeckia glabrescens extract as an active ingredient.
상기 의약외품은 피부외용제 및 개인위생용품을 포함할 수 있다. 예를 들어, 소독청결제, 샤워폼, 가그린, 물티슈, 세제비누, 핸드워시, 또는 연고제일 수 있으나 이에 제한되지는 않는다.The quasi-drugs may include external skin preparations and personal hygiene products. For example, it may be a disinfectant cleaner, shower foam, garnish, wet tissue, detergent soap, hand wash, or ointment, but is not limited thereto.
본 발명에 따른 상기 의약외품 조성물을 의약외품 첨가물로 사용할 경우, 상기 조성물을 그대로 첨가하거나 다른 의약외품 또는 의약외품 성분과 함께 사용 할 수 있고, 통상적인 방법에 따라 적절하게 사용할 수 있다. 유효성분의 혼합량은 사용 목적에 따라 적합하게 결정될 수 있다.When using the quasi-drug composition according to the present invention as a quasi-drug additive, the composition can be added as is or used with other quasi-drugs or quasi-drug components, and can be used appropriately according to conventional methods. The mixing amount of the active ingredient can be appropriately determined depending on the purpose of use.
본 발명의 의약외품 조성물은 일 예로, 일반적인 유화 제형 및 가용화 제형의 형태로 제조된 것일 수 있다. 예를 들어, 로션 등과 같은 유액, 크림, 연고, 스프레이, 오일젤, 젤, 오일, 에어로졸과 같은 제형을 가질 수 있으나, 본 발명의 코로나바이러스 감염증의 예방 또는 개선 효과를 나타내는 것이라면 제한되지 않고 사용할 수 있다. For example, the quasi-drug composition of the present invention may be manufactured in the form of a general emulsified formulation or solubilized formulation. For example, it may have a formulation such as lotion, cream, ointment, spray, oil gel, gel, oil, or aerosol, but can be used without limitation as long as it shows the effect of preventing or improving the coronavirus infection of the present invention. there is.
또한, 상기 의약외품 조성물은 각각의 제형에 일반적으로 의약외품 조성물에 배합되는 유분, 물, 계면활성제, 보습제, 탄소수 1 내지 4의 저급 알코올, 증점제, 킬레이트제, 색소, 방부제 또는 향료 등을 필요에 따라 적절히 배합하여 사용할 수 있다.In addition, the quasi-drug composition contains oil, water, surfactants, moisturizers, lower alcohols with 1 to 4 carbon atoms, thickeners, chelating agents, colorants, preservatives or fragrances, etc., which are generally mixed in quasi-drug compositions, as appropriate for each dosage form. Can be used in combination.
또한, 본 발명은 희렴(Siegesbeckia glabrescens) 추출물을 유효성분으로 포함하는 코로나바이러스 감염증의 예방 또는 개선용 화장료 조성물을 제공한다. In addition, the present invention provides a cosmetic composition for preventing or improving coronavirus infection containing Siegesbeckia glabrescens extract as an active ingredient.
본 발명의 화장료 조성물은 상기 추출물 이외에 화장료 조성물에 통상적으로 이용되는 성분들이 포함되며, 예컨대 항산화제, 안정화제, 용해화제, 비타민, 안료 및 향료와 같은 통상적인 보조제, 그리고 담체를 포함한다.In addition to the extract, the cosmetic composition of the present invention includes ingredients commonly used in cosmetic compositions, such as antioxidants, stabilizers, solubilizers, vitamins, conventional auxiliaries such as pigments and fragrances, and carriers.
본 발명의 화장료 조성물은 당 업계에서 통상적으로 제조되는 어떠한 제형으로도 제조될 수 있으며, 예를 들어, 용액, 현탁액, 유탁액, 페이스트, 겔, 크림, 로션, 파우더, 비누, 계면활성제-함유 클렌징, 오일, 분말 파운데이션, 유탁액 파운데이션, 왁스 파운데이션 및 스프레이 등으로 제형화될 수 있으나, 이에 한정되는 것은 아니다. 보다 상세하게는, 유연 화장수(스킨), 영양 화장수(밀크로션), 영양 크림, 맛사지 크림, 에센스, 아이크림, 클렌징 크림, 클렌징 포옴, 클렌징 워터, 샴푸, 팩, 스프레이 또는 파우더의 제형으로 제조될 수 있다.The cosmetic composition of the present invention can be prepared in any formulation commonly prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing products. , oil, powder foundation, emulsion foundation, wax foundation, spray, etc., but is not limited thereto. More specifically, it can be manufactured in the form of flexible lotion (skin), nourishing lotion (milk lotion), nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, shampoo, pack, spray or powder. You can.
본 발명의 제형이 페이스트, 크림 또는 겔인 경우에는 담체 성분으로서 동물성 유, 식물성 유, 왁스, 파라핀, 전분, 트라가칸타, 셀룰로오스 유도체, 폴리에틸렌 글리콜, 실리콘, 벤토나이트, 실리카, 탈크 또는 산화아연 등이 이용될 수 있다.When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tragacantha, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide are used as carrier ingredients. It can be.
본 발명의 제형이 파우더 또는 스프레이인 경우에는 담체 성분으로서 락토스, 탈크, 실리카, 알루미늄 히드록시드, 칼슘 실리케이트 또는 폴리아미드 파우더가 이용될 수 있고, 특히 스프레이인 경우에는 추가적으로 클로로플루오로히드로카본, 프로판/부탄 또는 디메틸 에테르와 같은 추진체를 포함할 수 있다.When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder can be used as the carrier ingredient. In particular, when the formulation is a spray, chlorofluorohydrocarbon and propane may be used as carrier ingredients. /May contain propellants such as butane or dimethyl ether.
본 발명의 제형이 용액 또는 유탁액인 경우에는 담체 성분으로서 용매, 용해화제 또는 유탁화제가 이용되고, 예컨대 물, 에탄올, 이소프로판올, 에틸 카보네이트, 에틸 아세테이트, 벤질 알코올, 벤질 벤조에이트, 프로필렌글리콜, 1,3-부틸글리콜 오일, 글리세롤 지방족 에스테르, 폴리에틸렌 글리콜 또는 소르비탄의 지방산 에스테르가 있다.When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent, or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 , 3-butyl glycol oil, glycerol aliphatic esters, polyethylene glycol or fatty acid esters of sorbitan.
본 발명의 제형이 현탁액인 경우에는 담체 성분으로서 물, 에탄올 또는 프로필렌 글리콜과 같은 액상의 희석제, 에톡실화 이소스테아릴 알코올, 폴리옥시에틸렌 소르비톨 에스테르 및 폴리옥시에틸렌 소르비탄 에스테르와 같은 현탁제, 미소 결정성 셀룰로오스, 알루미늄 메타히드록시드, 벤토나이트, 아가 또는 트라가칸타 등이 이용될 수 있다.When the formulation of the present invention is a suspension, the carrier ingredients include water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, and microcrystals. Cellulose, aluminum metahydroxide, bentonite, agar or tragacantha can be used.
본 발명의 제형이 계면-활성제 함유 클렌징인 경우에는 담체 성분으로서 지방족 알코올 설페이트, 지방족 알코올 에테르 설페이트, 설포숙신산 모노에스테르, 이세티오네이트, 이미다졸리늄 유도체, 메틸타우레이트, 사르코시네이트, 지방산 아미드 에테르 설페이트, 알킬아미도베타인, 지방족 알코올, 지방산 글리세리드, 지방산 디에탄올아미드, 식물성 유, 라놀린 유도체 또는 에톡실화 글리세롤 지방산 에스테르 등이 이용될 수 있다.When the formulation of the present invention is a surfactant-containing cleansing agent, the carrier ingredients include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, and fatty acid amide. Ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester can be used.
또한, 본 발명은 희렴(Siegesbeckia glabrescens) 추출물을 유효성분으로 포함하는 코로나바이러스 감염증의 예방 또는 개선용 사료 첨가제를 제공한다.In addition, the present invention provides a feed additive for preventing or improving coronavirus infection containing Siegesbeckia glabrescens extract as an active ingredient.
본 발명의 사료 첨가제는 사료관리법상의 보조사료에 해당한다. 본 발명에서 용어 '사료'는 동물이 먹고, 섭취하며, 소화시키기 위한 또는 이에 적당한 임의의 천연 또는 인공 규정식, 한끼식 등 또는 상기 한끼식의 성분을 의미할 수 있다. 상기 사료의 종류는 특별히 제한되지 아니하며, 당해 기술 분야에서 통상적으로 사용되는 사료를 사용할 수 있다. 상기 사료의 비제한적인 예로는, 곡물류, 근과류, 식품 가공 부산물류, 조류, 섬유질류, 제약 부산물류, 유지류, 전분류, 박류 또는 곡물 부산물류 등과 같은 식물성 사료; 단백질류, 무기물류, 유지류, 광물성류, 유지류, 단세포 단백질류, 동물성 플랑크톤류 또는 음식물 등과 같은 동물성 사료를 들 수 있다. 이들은 단독으로 사용되거나 2종 이상을 혼합하여 사용될 수 있다.The feed additive of the present invention corresponds to supplementary feed under the Feed Management Act. In the present invention, the term 'feed' may mean any natural or artificial diet, meal, etc., or a component of the meal, for or suitable for eating, ingestion, and digestion by animals. The type of feed is not particularly limited, and feed commonly used in the art can be used. Non-limiting examples of the feed include plant feeds such as grains, roots and fruits, food processing by-products, algae, fiber, pharmaceutical by-products, oils and fats, starches, cucurbits or grain by-products; Examples include animal feeds such as proteins, inorganic substances, fats and oils, minerals, oils and fats, single-cell proteins, zooplanktons or food. These may be used alone or in combination of two or more types.
이하, 제조예 및 실시예를 이용하여 본 발명을 더욱 상세하게 설명하고자 한다. 이들 제조예 및 실시예는 오로지 본 발명을 보다 구체적으로 설명하기 위한 것으로, 본 발명의 범위가 이들에 의해 제한되지 않는다는 것은 당해 기술분야에서 통상의 지식을 가진 자에게 있어 자명한 것이다. Hereinafter, the present invention will be described in more detail using manufacturing examples and examples. These manufacturing examples and examples are only for illustrating the present invention in more detail, and it is obvious to those skilled in the art that the scope of the present invention is not limited thereto.
제조예 1. 희렴 추출물의 제조Preparation Example 1. Preparation of Heeryeom Extract
희렴은 광명당제약으로부터 규격품을 구입하였으며, 구체적으로, 희렴 건조 원물 1 중량부에 대하여 10 중량부의 물(100℃)을 첨가한 후, 3시간 동안 환류 추출한 다음 거름망(추출포)에 여과한 후 53㎛ 필터에 통과시킨 통과물을 감압 농축한 다음 동결건조하여 희렴 추출물을 제조하였다.Heeryum purchased a standard product from Gwangmyeongdang Pharmaceutical. Specifically, 10 parts by weight of water (100°C) was added to 1 part by weight of Heeryum dried raw material, extracted under reflux for 3 hours, and then filtered through a strainer (extraction cloth). The material passed through a 53㎛ filter was concentrated under reduced pressure and then freeze-dried to prepare a rare extract.
실시예 1. 항바이러스 활성평가: CPE(cytopathic effect) 저해 어세이 시스템 구축Example 1. Evaluation of antiviral activity: Construction of CPE (cytopathic effect) inhibition assay system
96웰 플레이트에 Vero E6 세포를 배양한 후, SARS-CoV2를 m.o.i 0.01로 접종하였다. 접종 후, 상기 제조예 1의 희렴 추출물을 2%(v/v) FBS 및 1%(v/v) 페니실린-스트렙토마이신이 포함된 DMEM으로 희석하여 3일 동안 배양하였다. 배양 후, 세포 배양액을 제거한 다음 메탄올:아세톤이 1:1 부피비로 혼합된 용액을 처리한 다음 실온에서 10분 동안 고정하였다. 이후, 용액을 제거하고, 0.4%(w/v) 크리스탈 바이올렛(crystal violet)을 각 웰에 첨가한 다음 10분 동안 실온에서 염색한 후, 용액을 모두 제거하고 건조하였다. 건조 후, 메탄올:아세트산을 1:1 부피비로 혼합한 후, 3차 증류수로 5배 희석한 용액을 각 웰에 100㎕씩 첨가한 후 2시간 동안 크리스탈 바이올렛을 추출한 다음 550nm에서 흡광도를 측정하였다. After culturing Vero E6 cells in a 96-well plate, SARS-CoV2 was inoculated at an m.o.i of 0.01. After inoculation, the rare extract of Preparation Example 1 was diluted with DMEM containing 2% (v/v) FBS and 1% (v/v) penicillin-streptomycin and cultured for 3 days. After incubation, the cell culture medium was removed, and then treated with a 1:1 volume ratio of methanol:acetone and fixed for 10 minutes at room temperature. Afterwards, the solution was removed, 0.4% (w/v) crystal violet was added to each well, and the cells were stained at room temperature for 10 minutes, then all of the solution was removed and dried. After drying, methanol:acetic acid was mixed at a 1:1 volume ratio, and 100 ㎕ of a solution diluted 5 times with distilled water was added to each well. Crystal violet was extracted for 2 hours, and the absorbance was measured at 550 nm.
그 결과, 도 1에 개시된 바와 같이 본 발명의 희렴 추출물은 SARS-CoV2의 증식을 억제하였고, IC50 값이 90.36㎍/mL인 것을 확인하여, 희렴 추출물은 SARS-CoV2 바이러스로부터 세포를 보호하는 항바이러스 효과가 우수하였다.As a result, as shown in Figure 1, the Heereum extract of the present invention inhibited the proliferation of SARS-CoV2, and the IC 50 value was confirmed to be 90.36 ㎍/mL, indicating that the Heereum extract is an antibody that protects cells from the SARS-CoV2 virus. The virus effect was excellent.
실시예 2. 희렴 추출물의 바이러스 복제 억제 효과 확인 Example 2. Confirmation of viral replication inhibitory effect of Heereum extract
희렴 추출물의 바이러스 복제 억제 효과를 qPCR로 확인하였다. 구체적으로, 96웰 플레이트에 Vero E6 세포를 배양한 후, SARS-CoV2를 m.o.i 0.01로 접종하고, 농도별 제조예 1의 시료를 처리한 후, 24시간 동안 배양하였다. 배양 후, 바이오니아사의 AccuPrep Viral RNA Extraction kit를 사용하여 세포 밖으로 방출된 바이러스 RNA를 추출하였다. 이어서 토요보사의 ReverTra Ace qPCR RT kit를 사용하여 cDNA를 합성한 다음 스파이크단백질의 RBD에 대한 특이적인 프라이머로 PCR을 수행하고 바이러스 RNA 복제 수(viral RNA copy number)를 스파이크 단백질 유전자 플라스미드 표준 시료를 이용해 계산하였다. The viral replication inhibitory effect of Heereum extract was confirmed by qPCR. Specifically, after culturing Vero E6 cells in a 96-well plate, SARS-CoV2 was inoculated at an m.o.i of 0.01, samples of Preparation Example 1 were treated at different concentrations, and cultured for 24 hours. After incubation, viral RNA released out of the cells was extracted using Bioneer's AccuPrep Viral RNA Extraction kit. Then, cDNA was synthesized using Toyobo's ReverTra Ace qPCR RT kit, then PCR was performed with specific primers for the RBD of the spike protein, and the viral RNA copy number was measured using the spike protein gene plasmid standard sample. Calculated.
그 결과, 도 2에 개시된 바와 같이 희렴 추출물은 SARS-CoV2 바이러스의 복제를 억제하는 효과가 우수하였다. As a result, as shown in Figure 2, the extract of Heereum was excellent in inhibiting the replication of the SARS-CoV2 virus.
실시예 3. 희렴 추출물의 바이러스 감염 억제 효과 확인 Example 3. Confirmation of the viral infection inhibition effect of Heereum extract
희렴 추출물을 이용하여 SARS-CoV2 바이러스 감염 억제 효과를 면역형광분석법으로 확인하였다. 구체적으로, Vero E6 세포를 1×104 세포/웰로 플레이트에 분주하고, 농도별 제조예 1의 시료를 처리한 후, 2%(v/v) FBS 및 1%(v/v) 항생제가 포함된 DMEM으로 1000배 희석한 SARS-CoV2를 처리한 다음 24시간 동안 배양하였다. 배양 후, 4%(v/v) 포름알데히드(formaldehyde) 용액으로 세포를 고정한 다음 2000배 희석한 1차 항체(Nucleocapsid 단백질)와 반응시킨 후, 200배 희석한 2차 항체와 반응시켰다. 반응 후, 세포를 형광현미경으로 관찰하였고, 형광강도를 분석하였다. The inhibitory effect of SARS-CoV2 virus infection using Heereum extract was confirmed using immunofluorescence analysis. Specifically, Vero E6 cells were distributed on a plate at 1×10 4 cells/well, and samples of Preparation Example 1 were processed at each concentration, followed by 2% (v/v) FBS and 1% (v/v) antibiotics. SARS-CoV2 diluted 1000 times was treated with DMEM and cultured for 24 hours. After incubation, the cells were fixed with a 4% (v/v) formaldehyde solution, reacted with a primary antibody (Nucleocapsid protein) diluted 2000 times, and then reacted with a secondary antibody diluted 200 times. After the reaction, the cells were observed under a fluorescence microscope, and the fluorescence intensity was analyzed.
그 결과, 도 3에 개시된 바와 같이 희렴 추출물 처리에 의해 SARS-CoV2 바이러스의 세포 내 감염이 효과적으로 억제되었다.As a result, as shown in Figure 3, intracellular infection of the SARS-CoV2 virus was effectively inhibited by treatment with the rare extract.

Claims (10)

  1. 희렴(Siegesbeckia glabrescens) 추출물을 유효성분으로 포함하는 코로나바이러스에 대한 항바이러스용 조성물.An antiviral composition for coronavirus containing Siegesbeckia glabrescens extract as an active ingredient.
  2. 제1항에 있어서, 상기 코로나바이러스는 제2형 중증급성호흡기증후군 코로나바이러스(SARS-CoV2)인 것을 특징으로 하는 코로나바이러스에 대한 항바이러스용 조성물.The composition for antiviral use against coronavirus according to claim 1, wherein the coronavirus is type 2 severe acute respiratory syndrome coronavirus (SARS-CoV2).
  3. 제1항에 있어서, 상기 희렴 추출물의 추출용매는 물, C1~C4의 저급 알코올 또는 이들의 혼합물인 것을 특징으로 하는 코로나바이러스에 대한 항바이러스용 조성물.The antiviral composition for coronavirus according to claim 1, wherein the extraction solvent of the rare extract is water, a lower alcohol of C 1 to C 4 , or a mixture thereof.
  4. 희렴(Siegesbeckia glabrescens) 추출물을 유효성분으로 포함하는 코로나바이러스 감염증의 예방 또는 치료용 약학 조성물.A pharmaceutical composition for preventing or treating coronavirus infection, comprising Siegesbeckia glabrescens extract as an active ingredient.
  5. 제4항에 있어서, 상기 희렴 추출물 이외에 약학적으로 허용 가능한 담체, 부형제 또는 희석제를 더 포함하는 것을 특징으로 하는 코로나바이러스 감염증의 예방 또는 치료용 약학 조성물.The pharmaceutical composition for preventing or treating coronavirus infection according to claim 4, further comprising a pharmaceutically acceptable carrier, excipient, or diluent in addition to the rare extract.
  6. 희렴(Siegesbeckia glabrescens) 추출물을 유효성분으로 포함하는 코로나바이러스 감염증의 예방 또는 개선용 건강기능식품 조성물.A health functional food composition for preventing or improving coronavirus infection containing Siegesbeckia glabrescens extract as an active ingredient.
  7. 제6항에 있어서, 상기 조성물은 분말, 과립, 환, 정제, 캡슐, 캔디, 시럽 및 음료 중에서 선택된 어느 하나의 제형으로 제조되는 것을 특징으로 하는 코로나바이러스 감염증의 예방 또는 개선용 건강기능식품 조성물.The health functional food composition for preventing or improving coronavirus infection according to claim 6, wherein the composition is manufactured in any one formulation selected from powder, granule, pill, tablet, capsule, candy, syrup, and beverage.
  8. 희렴(Siegesbeckia glabrescens) 추출물을 유효성분으로 포함하는 코로나바이러스 감염증의 예방 또는 개선용 의약외품 조성물.A quasi-drug composition for preventing or improving coronavirus infection containing Siegesbeckia glabrescens extract as an active ingredient.
  9. 희렴(Siegesbeckia glabrescens) 추출물을 유효성분으로 포함하는 코로나바이러스 감염증의 예방 또는 개선용 화장료 조성물.A cosmetic composition for preventing or improving coronavirus infection containing Siegesbeckia glabrescens extract as an active ingredient.
  10. 희렴(Siegesbeckia glabrescens) 추출물을 유효성분으로 포함하는 코로나바이러스 감염증의 예방 또는 개선용 사료 첨가제.A feed additive for preventing or improving coronavirus infection containing Siegesbeckia glabrescens extract as an active ingredient.
PCT/KR2023/008574 2022-06-22 2023-06-21 Composition for prevention, amelioration or treatment of coronavirus infection comprising medicinal herb extract as active ingredient WO2023249393A1 (en)

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20050121094A (en) * 2004-06-21 2005-12-26 에스케이케미칼주식회사 Preventive and curative extracts from siegesbeckiae herba for respiratory organ disease
KR20100055030A (en) * 2008-11-17 2010-05-26 안국약품 주식회사 Composition containing a herbal extract for preventing and treating respiratory organ disease
KR20190009838A (en) * 2011-04-21 2019-01-29 마리 케이 인코포레이티드 Topical skin care formulations comprising plant extracts
KR102368892B1 (en) * 2021-05-20 2022-03-03 주식회사 유진큐어 A composition for anti-inflammatory comprising extracts of single leaf cremastra and sigesbeckia glabrescens makino
KR20220044084A (en) * 2020-09-28 2022-04-06 한국 한의학 연구원 Composition for preventing or treating corona virus comprising Hoveniae Semen Cum Fructus extracts
KR102524071B1 (en) * 2022-06-22 2023-04-21 한국 한의학 연구원 Composition for preventing, ameliorating or treating coronavirus infectious disease comprising herbal medicine extract as effective component

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20050121094A (en) * 2004-06-21 2005-12-26 에스케이케미칼주식회사 Preventive and curative extracts from siegesbeckiae herba for respiratory organ disease
KR20100055030A (en) * 2008-11-17 2010-05-26 안국약품 주식회사 Composition containing a herbal extract for preventing and treating respiratory organ disease
KR20190009838A (en) * 2011-04-21 2019-01-29 마리 케이 인코포레이티드 Topical skin care formulations comprising plant extracts
KR20220044084A (en) * 2020-09-28 2022-04-06 한국 한의학 연구원 Composition for preventing or treating corona virus comprising Hoveniae Semen Cum Fructus extracts
KR102368892B1 (en) * 2021-05-20 2022-03-03 주식회사 유진큐어 A composition for anti-inflammatory comprising extracts of single leaf cremastra and sigesbeckia glabrescens makino
KR102524071B1 (en) * 2022-06-22 2023-04-21 한국 한의학 연구원 Composition for preventing, ameliorating or treating coronavirus infectious disease comprising herbal medicine extract as effective component

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHOI YOUN HAK ET AL.: "PE-12: Anti-inflammatory and Antioxidative Activities of Siegesbeckia glabrescens and Siegesbeckia pubescens ", ABSTRACTS OF KOREAN SOCIETY OF SOIL SCIENCE AND FERTILIZER FALL CONFERENCE, KOREA, 1 January 2014 (2014-01-01), Korea, pages 251, XP009551997 *
JOO-SHIL ET AL.: "Screening of Anti-HIV-1 Activity of Natural Products by MTT Assay", THE JOURNAL OF THE KOREAN SOCIETY OF VIROLOGY, vol. 27, no. 1, 1997, pages 87 - 95 *
NASIR NAURAH NABIHAH, SEKAR MAHENDRAN, FULORIA SHIVKANYA, GAN SIEW HUA, RANI NUR NAJIHAH IZZATI MAT, RAVI SUBBAN, BEGUM M. YASMIN,: "Kirenol: A Potential Natural Lead Molecule for a New Drug Design, Development, and Therapy for Inflammation", MOLECULES, MDPI AG, CH, vol. 27, no. 3, 23 January 2022 (2022-01-23), CH , pages 734, XP093121064, ISSN: 1420-3049, DOI: 10.3390/molecules27030734 *

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