WO2022237181A1 - 一种dna、rna核酸共提取及检测系统 - Google Patents
一种dna、rna核酸共提取及检测系统 Download PDFInfo
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- WO2022237181A1 WO2022237181A1 PCT/CN2021/141190 CN2021141190W WO2022237181A1 WO 2022237181 A1 WO2022237181 A1 WO 2022237181A1 CN 2021141190 W CN2021141190 W CN 2021141190W WO 2022237181 A1 WO2022237181 A1 WO 2022237181A1
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- assembly
- extraction
- amplification
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Definitions
- the purpose of the present invention is to provide a DNA, RNA nucleic acid co-extraction and detection system, which realizes the automation of barcode scanning, sample addition, incubation extraction, transfer mixing and detection in the extraction and amplification process, effectively The efficiency of nucleic acid extraction and amplification is improved.
- the present invention provides the following technical solutions:
- a DNA and RNA nucleic acid co-extraction and detection system including a nucleic acid extraction system, a sealing transfer mixing system, a nucleic acid detection system and a control system, the nucleic acid extraction system, the sealing transfer mixing system and the nucleic acid detection system are all Connect with the control system;
- the nucleic acid extraction system includes a basket for loading extraction reagent strips and sample tubes, a consumable supply module, a kit loading module, a barcode scanning device for automatically entering barcode information, a magnetic incubation device, and a Extract the reaction chamber of the reagent strip or the automatic sampling device for pipetting in the PCR tube and sealing the PCR tube;
- the sealing transfer system includes a PCR transfer device and a high-speed mixing device for grabbing and driving the PCR tube to move;
- the basket includes a basket cover plate, a basket bottom plate for holding extraction reagent strips, and a sample seat for holding sample tubes, and the sample seat is detachably connected to the bottom plate of the basket;
- the basket cover plate is rotatably connected to the basket bottom plate, so as to limit the displacement of the extraction reagent strip in the height direction when the basket cover plate is attached to the basket bottom plate.
- the barcode scanning device includes a mounting bracket, a scanning assembly, a push handle assembly for pushing and extracting reagent strips between the code scanning position and the standby position, and a push handle that drives the push handle assembly to move along the length direction of the mounting bracket
- a moving component and a detection component the detection component is used to determine whether there are extraction reagent strips and sample tubes in the current channel, the detection component is connected to the pusher moving component with signals, the scanning component and the detection component are installed in the The mounting base of the push handle assembly described above.
- the magnetic incubator includes an incubation module for heating the reaction chamber, a magnetic module for magnetically attracting the reaction chamber, and a drive assembly, the incubation module, the magnetic module Both are connected with the driving assembly, and the driving assembly is used to drive the incubation module and the magnetic attraction module to approach the reaction chamber alternately.
- the incubation module includes a heating block provided with a reagent tank, a heating assembly attached to the inner wall of the reagent tank, a heat dissipation assembly arranged at the bottom of the heating block, and a heating block for detecting the temperature of the heating block.
- a temperature sensor connected to the heating block.
- the magnetic attraction module includes a magnet and a rotating assembly for driving the magnet to rotate, and the rotating assembly is connected with the magnet;
- the magnet includes a first magnetic attraction surface and a second magnetic attraction surface, the first magnetic attraction surface and the second magnetic attraction surface have different areas and are used to absorb the magnetic beads in the reaction chamber.
- the PCR transfer device includes a gripper assembly for grasping or loosening the PCR tube, a cap opening assembly for opening or closing the amplification cover of the PCR amplification device, and a cap opening assembly for driving the gripper
- a gripper assembly for grasping or loosening the PCR tube
- a cap opening assembly for opening or closing the amplification cover of the PCR amplification device
- a cap opening assembly for driving the gripper
- An X-axis assembly for moving the assembly and the cover opening assembly along the X-axis direction
- a Y-axis assembly for driving the handle assembly and the cover opening assembly to move along the Y-axis direction
- a Y-axis assembly for driving the handle assembly and the cover opening assembly to move along the Y-axis direction.
- a Z-axis assembly that moves the cover-opening assembly along the Z-axis direction;
- the gripper assembly includes a gripper bottom plate, a gripper movable plate arranged in parallel under the gripper bottom plate, a gripper for grabbing PCR tubes, and a handle for controlling the opening or contraction of the gripper.
- a retractable drive assembly, the retractable drive assembly is arranged on the handle bottom plate, and the telescopic end of the retractable drive assembly passes through the handle bottom plate and is connected with the handle movable plate to drive the handle movable plate rise or fall;
- the bottom plate of the gripper and the movable plate of the gripper are both connected to the retraction drive assembly, and the gripper is opened when the movable plate of the gripper is lowered, and the gripper is opened when the movable plate of the gripper is raised. shrink.
- the PCR amplification device includes an amplification heating block, an amplification heating sheet, an amplification radiator, an amplification cover for covering the amplification heating block, and an amplification cover for raising the amplification radiator.
- An amplification cooling assembly with heat dissipation efficiency, the amplification heating block is provided with at least two amplification holes for placing PCR tubes, the amplification cover, the amplification heating block, the amplification heating sheet and the The amplification radiators are arranged sequentially from top to bottom.
- the fluorescence detection device includes a light source assembly for providing a fluorescence light source, a fluorescence detection assembly for quantitative detection, a light source optical fiber, and a fluorescence detection optical fiber, one side of the amplification hole is connected to one end of the light source optical fiber connected, the other side of the amplification hole is connected to one end of the fluorescence detection optical fiber;
- the other end of the light source fiber is connected to the light source assembly, and the other end of the fluorescence detection fiber is connected to the fluorescence detection assembly.
- the reagent needle assembly includes a reagent needle, a pricking control assembly, a pipetting control assembly, a reagent needle lifting assembly and a reagent needle translation assembly
- the reagent needle includes an adapter and a reagent pump connected to the adapter
- the The reagent pump is connected to the pipetting control component
- the adapter is connected to the tying control component, so that the tying control component controls the adapter to puncture or withdraw from the tip or sealing plug;
- the reagent needle translation assembly is used to drive the reagent needle to move in the horizontal plane and vertical to the moving direction of the gantry assembly, and the reagent needle lifting assembly is used to drive the reagent needle to move in the vertical direction.
- the DNA and RNA nucleic acid co-extraction and detection system When the DNA and RNA nucleic acid co-extraction and detection system provided by the present invention work, first load the sample tubes and extraction reagent strips containing the samples to be tested at the basket, and use the barcode scanning device to input the barcode information of the sample tubes and extraction reagent strips; After completion, the automatic sampling device punctures the sealing film of the extraction reagent strip, and injects the extraction reagent and the sample to be tested into the reaction chamber of the extraction reagent strip; Nucleic acid in the sample to be tested; the automatic sampling device fills the PCR tube with the amplification reagent and the purified product after incubation and magnetic absorption, and seals the PCR tube with a sealing plug; after sealing, the PCR transfer device grabs the PCR tube and transfers it Transfer to the high-speed mixing device; after mixing, the PCR transfer device transfers the PCR tube to the PCR amplification device to complete the PCR amplification process; after the amplification process, the flu
- the DNA and RNA co-extraction and amplification system realizes the automation of barcode scanning, sample addition, incubation extraction, transfer mixing and fluorescence detection during the detection process, and effectively improves the efficiency of nucleic acid extraction and amplification.
- Fig. 1 is the structural schematic diagram of the specific embodiment of DNA, RNA nucleic acid co-extraction and detection system provided by the present invention
- Fig. 2 is the structural representation of carrying basket
- Fig. 3 is the explosion schematic diagram of carrying basket
- Fig. 4 is buckle assembly on the workbench
- Fig. 5 is a schematic structural diagram of the pressing assembly on the workbench
- Fig. 6 is a schematic structural diagram of a tip box for disposable consumables
- Fig. 7 is a schematic structural diagram of a PCR consumable box
- Fig. 8 is a schematic structural diagram of a barcode scanning device
- Fig. 1 is the structural schematic diagram of the specific embodiment of DNA, RNA nucleic acid co-extraction and detection system provided by the present invention
- Fig. 2 is the structural representation of carrying basket
- Fig. 3 is the explosion schematic diagram of carrying basket
- Fig. 4 is buckle assembly on the workbench
- Fig. 5 is a schematic structural diagram of the pressing assembly on the workbe
- FIG. 20 is a schematic diagram of the structure of the sample needle assembly
- FIG. 21 is a schematic diagram of the structure of the PCR transfer device
- FIG. 22 is a schematic diagram of the structure of the Z-axis assembly
- 23 is a schematic structural view of the gripper assembly
- FIG. 24 is a schematic exploded view of a PCR amplification device
- FIG. 25 is a schematic structural view of a fluorescence detection device.
- the core of the present invention is to provide a DNA and RNA nucleic acid co-extraction and detection system, which realizes the automation of barcode scanning, sample addition, incubation extraction, transfer mixing and detection in the extraction and amplification process, and effectively improves the efficiency of nucleic acid extraction. Amplification efficiency.
- the X-axis direction is the movement direction of the gantry assembly 173 of the automatic sample loading device 17
- the Y-axis direction is the direction perpendicular to the X-axis direction in the horizontal plane
- the Z-axis direction is the vertical direction.
- a DNA and RNA nucleic acid co-extraction and detection system include a nucleic acid extraction system, a sealing transfer mixing system and a nucleic acid detection system;
- the nucleic acid extraction system includes a basket 11 for loading extraction reagent strips 03 and sample tubes 04,
- the consumable supply module, the reagent box loading module 14, the barcode scanning device 15 for automatically entering barcode information, the magnetic suction incubation device 16, and the reaction chamber 031 or PCR tube 021 for extracting the reagent strip 03 and sealing it
- the sealing transfer system includes a PCR transfer device 21 and a high-speed mixing device 22 for grabbing and driving the PCR tube 021 to move;
- the nucleic acid detection system includes a PCR amplification device 31 and a fluorescence detection device 32 .
- the consumable supply module is used to hold the disposable consumables required in the detection process, such as tip01 and PCR tube 021.
- the consumables are picked and placed by the automatic sample loading device 17 and the PCR transfer device 21, without manual operation by the detection personnel. Effectively reduce the work intensity of testing personnel.
- the consumable supply module includes a disposable tip consumable box 12 for containing tip01 and a PCR consumable box 13 for containing PCR tube 021 and its corresponding sealing plug 022 .
- the structure, material, size and position of the disposable tip consumable box 12 and the PCR consumable box 13 are determined according to actual testing needs.
- the kit loading module 14 is used to place a variety of amplification reagents required for the construction of the PCR system.
- the structure, material, size and position of the kit loading module 14 are determined according to actual detection needs.
- the magnetic suction incubator 16 carries out the incubation and magnetic suction treatment on the mixed solution, and extracts the nucleic acid in the mixed solution; After the liquid transfer is completed, use the sealing plug 022 to seal the PCR tube 021; after the sealing is completed, the PCR transfer device 21 grabs the PCR tube 021 and transfers it to the high-speed mixing device 22 for high-speed mixing; After mixing, the PCR transfer device 21 transfers the PCR tube 021 to the PCR amplification device 31 to complete the
- the DNA and RNA co-extraction and amplification system provided in this example realizes the automation of barcode scanning, sample addition, incubation extraction, transfer mixing and fluorescence detection during the detection process, effectively improving the efficiency of nucleic acid extraction and amplification.
- the nucleic acid extraction system is also provided with a pocket fluid suction device 18, which includes a pocket fluid box for collecting dripped reagents during pipetting and a ventilation device for ventilating the suction liquid level.
- the pocket liquid box can effectively prevent cross-contamination caused by reagents dripping on the operation plane; the exhaust device can extract the polluted gas generated by the injection liquid to avoid pollution caused by aerosol diffusion.
- the basket 11 includes a basket cover plate 112, a basket bottom plate 111 for holding the extraction reagent strip 03, and a sample seat 113 for holding the sample tube 04, and the sample seat 113 and the basket bottom plate 111 are detachable Connection; the basket cover plate 112 is rotatably connected to the basket bottom plate 111, so as to limit the displacement of the extraction reagent strip 03 in the height direction when the basket cover plate 112 is attached to the basket bottom plate 111.
- the center of the basket base plate 111 is provided with several reagent strip installation holes arranged parallel to its length direction, and the sample seat 113 is provided with several sample tube installation holes; the size and size of the reagent strip installation holes and the sample tube installation holes The quantity is determined according to actual testing needs.
- the basket cover plate 112 is rotatably connected with the basket bottom plate 111 to prevent the extraction reagent strip 03 from moving in the height direction of the basket 11.
- the basket cover plate 112 is rotatably connected to one end of the basket bottom plate 111 in the length direction;
- the plate 112 is provided with reagent strip positioning holes corresponding to the reagent strip installation holes one by one.
- the top surface of the basket bottom plate 111 is provided with a first magnet
- the bottom plate of the basket cover 112 is provided with a second magnet
- the first magnet and the second magnet are attracted together. Therefore, it is necessary to overcome the suction force between the first magnet and the second magnet when the extraction reagent strip 03 tilts or falls out, so as to realize the effective limit and accurate positioning of the extraction reagent strip 03 in the height direction of the basket 11.
- the first magnet and the second magnet can also be replaced by similar positioning structures such as positioning slots and positioning blocks that engage with each other.
- a support assembly 114 is provided on the bottom surface of the basket bottom plate 111.
- the support assembly 114 can specifically be an I-shaped structure as shown in FIG. 2 , or it can be arranged on the basket
- the support columns at the four corners of the bottom plate 111 can also be set as other common bracket structures.
- batch synchronous loading of extraction reagent strips 03 and sample tubes 04 is realized, which saves the cumbersome process of placing multiple times and has high placement efficiency; the basket 11 can store the extraction reagent strips 03 and sample tubes 04 Accurate positioning avoids repositioning the extraction reagent strip 03 and the sample tube 04 after the basket 11 is placed on the workbench.
- the workbench is provided with a buckle assembly 191 and a pressing assembly 192 for positioning the basket 11.
- the buckle assembly 191 and the pressing assembly 192 are engaged with the two ends of the basket 11 in the length direction respectively, and the buckle assembly
- the structure, size, material, quantity and installation position of 191 and pressing assembly 192 should be determined according to actual production needs, and will not be repeated here.
- the basket 11 also includes a basket positioning assembly 116, and the basket positioning assembly 116 includes a guide seat, a tension spring, a guide shaft arranged along the length direction of the bottom plate 111 of the basket, and a basket baffle for limiting the extraction of the reagent strip 03 ,
- the guide seat is connected to the bottom surface of the basket base plate 111;
- the guide shaft is slidably connected with the guide seat, one end of the guide shaft is vertically connected with the basket baffle plate, and the two ends of the tension spring are respectively connected with the basket baffle plate and the bottom surface of the basket base plate 111.
- the pusher assembly 154 drives the extraction reagent strip 03 to move from the standby position to the code scanning position; The information is scanned; when neither of them detects the barcode information, the pusher moving assembly 152 drives the pusher assembly 154 to move to the next channel.
- the detection component 155 first judges whether the extraction reagent strip 03 and the sample tube 04 exist in the current channel, and if so, the push handle component 154 drives the extraction reagent strip 03 to move to the code scanning position, and the scanning component 153 checks the extraction reagent strip 03 and the sample tube 04. /or the two-dimensional code of the sample tube 04 is scanned, and after the scanning is completed, the pusher assembly 154 drives the extraction reagent strip 03 to return to the standby position, and the pusher moving assembly 152 drives the pusher assembly 154 and the scanning assembly 153 to move to the next channel; if not, Then the pusher moving assembly 152 directly drives the pusher assembly 154 and the scanning assembly 153 to move to the next channel.
- the scanning assembly 153 and the push handle assembly 154 can move along the length direction of the mounting bracket 151, and can read multi-channel barcode information; the scanning assembly 153 can scan the extraction reagent strip 03, and can also scan the sample tube 04 scanning, the detection speed is fast; at the same time, the barcode scanning device 15 has a compact structure and can operate in a narrow and long space, which is conducive to reducing the size of the DNA and RNA nucleic acid co-extraction and detection system and improving space utilization.
- the pusher moving assembly 152 can be controlled to drive the pusher assembly 154 to reset, and the extraction reagent strips 03 and sample tubes 04 in each channel are sequentially detected from the first channel; the pusher can also be controlled
- the moving component 152 drives the scanning component 153 to scan in reverse order for the next group of extraction reagent strips 03 and sample tubes 04 to be detected.
- the detection assembly 155 may also include a scanning reset sensor for detecting whether the scanning assembly 153 has moved to the end of the mounting bracket 151 , and the scanning reset sensor is connected to the push handle moving assembly 152 in signal.
- the magnetic incubator 16 includes an incubation module 161 for heating the reaction chamber 031, a magnetic attraction module 162 and a drive assembly 163 for magnetically attracting the reaction chamber 031, and the incubation module 161
- the magnetic attraction modules 162 are all connected to the drive assembly 163, and the drive assembly 163 is used to drive the incubation module 161 and the magnetic attraction module 162 to approach the reaction chamber 031 alternately.
- the driving component 163 is controlled to make the incubation module 161 close to the reaction chamber 031, so as to effectively heat the mixed solution in the reaction chamber 031 and facilitate The mixture is incubated while the magnetic module 162 is away from the reaction chamber 031 to avoid affecting the incubation process; after the incubation process is completed, the drive assembly 163 is controlled to make the magnetic module 162 approach the reaction chamber 031, so that the The magnetic beads are adsorbed to the inner wall of the reaction chamber 031 , while the incubation module 161 is away from the reaction chamber 031 ; after the magnetic suction operation is completed, the driving assembly 163 is controlled to keep the magnetic suction module 162 away from the reaction chamber 031 .
- the driving assembly 163 is used to alternately control the incubation module 161 and the magnetic attraction module 162 to approach the reaction chamber 031, so as to realize the contact and separation of the incubation module 161 and the reaction chamber 031, and avoid the constant heating reaction of the incubation module 161
- the mixed liquid in the warehouse 031 causes the temperature of the mixed liquid to rise and fall too slowly, which improves the operating efficiency of the device; at the same time, the integrated setting of the incubation module 161 and the magnetic suction module 162 effectively reduces the overall size of the device and simplifies the temperature. Nurturing and magnetic suction operation steps.
- Drive assembly 163 can be set to various drive mechanisms such as lead screw drive mechanism, guide rail drive mechanism, preferably, drive assembly 163 is lead screw drive mechanism, lead screw drive mechanism can be vertically arranged as shown in Figure 11, also can according to temperature
- the overall layout of the magnetic incubation device 16 is arranged horizontally, as long as the driving assembly 163 can drive the incubation module 161 to lift up and down.
- the magnetic incubator 16 can also include a limit support assembly 164, the magnetic attraction module 162 and the drive assembly 163 are all connected to the limit support assembly 164, and the limit support assembly 164 is provided with a horizontal position for limiting the magnetic attraction module 162.
- the limit track of the moving track when the drive assembly 163 drives the incubation module 161 to rise, the magnetic attraction module 162 moves away from the reaction chamber 031 along the limit track; when the drive assembly 163 drives the incubation module 161 to descend, the magnetic attraction module 162 moves along the limit
- the bit track is close to reaction chamber 031.
- the limit support assembly 164 includes a horizontal support plate and a vertical support plate, and the horizontal support plate and the vertical support plate are vertically connected;
- the module 162 is slidably connected with the limit rail;
- the top of the vertical support plate is provided with a synchronous belt mechanism connected to the magnetic attraction module 162 along the vertical direction;
- a magnetic suction back spring can be set on the horizontal support plate, and the two ends of the magnetic suction back spring are respectively connected with the vertical support plate and the magnetic suction module 162;
- the magnetic suction moving assembly for component movement, the magnetic suction module 162 is installed on the magnetic suction moving assembly, and the magnetic suction moving assembly may specifically include a linear displacement mechanism such as a linear guide rail mechanism and a driving mechanism such as a screw motor and a hydraulic cylinder.
- the incubation module 161 includes a heating block 1611 provided with a reagent tank 1611-1, a heating assembly 1612 installed on the inner wall of the reagent tank 1611-1, and a heat dissipation assembly installed at the bottom of the heating block 1611 1613 and a temperature sensor 1614 for detecting the temperature of the heating block 1611 , the temperature sensor 1614 is connected to the heating block 1611 .
- reagent tanks 1611-1 are provided in the heating block 1611, so that the incubation temperature of multiple reaction chambers 031 is the same during heating, which solves the problem of temperature uniformity and is beneficial to improve The accuracy of the detection results and the reduction of experimental errors.
- the specific number of reagent tanks 1611-1 is determined according to actual needs, and will not be repeated here.
- the heating power of the heating assembly 1612 is distributed non-uniformly on the inner wall of the reagent tank 1611-1, so as to ensure the temperature uniformity of each position inside the heating block 1611.
- a thermal insulation component 1615 is provided on the outer periphery of the heating block 1611, and a cooling component 1616 is provided under the heat dissipation component 1613, so as to improve heat dissipation efficiency and facilitate arbitrary rapid switching of the incubation temperature within the incubation temperature range.
- heating block 1611 The structure, material, size, position and connection method of heating block 1611, heating component 1612, heat dissipation component 1613, temperature sensor 1614, heat preservation component 1615, and cooling component 1616 are determined according to actual incubation needs with reference to existing technologies, and will not be repeated here. repeat.
- the heating component 1612 heats the heating block 1611, and when the temperature sensor 1614 detects that the temperature of the heating block 1611 reaches the first preset temperature (usually set to 37°C), the heating of the heating component 1612 is stopped In operation, the heat preservation component 1615 keeps the temperature of the heating block 1611 constant, so as to incubate the sample solution in the reaction chamber 031 .
- the first preset temperature usually set to 37°C
- the magnetic attraction module 162 includes a magnet 1621 and a rotating assembly 1622 for driving the magnet 1621 to rotate.
- the rotating assembly 1622 is connected with the magnet 1621;
- the magnet 1621 includes a first magnetic surface 1621-1 and a second magnetic
- the suction surface 1621 - 2 , the first magnetic suction surface 1621 - 1 and the second magnetic suction surface 1621 - 2 have different areas and are used to absorb the magnetic beads in the reaction chamber 031 .
- the area of the first magnetic attraction surface 1621-1 is larger than the area of the second magnetic attraction surface 1621-2, so when the reaction chamber 031 needs a larger magnetic force, the control rotation assembly 1622 will move the first magnetic attraction surface 1621-1 is turned to face the mating surface of the reaction chamber 031; otherwise, when a small magnetic force is required, the rotating assembly 1622 is controlled to rotate the second magnetic attraction surface 1621-2 to the mating surface facing the reaction chamber 031.
- the rotating component 1622 realizes the transformation of magnetic force through the transformation of the magnetic attraction surface, and has a simple structure, convenient operation, and easy popularization.
- the magnetic suction module 162 is provided with a magnetic isolation plate 1623 for magnetic isolation of the magnetic beads during the non-magnetic suction process.
- the shape, size, material, connection method, connection position, etc. of the magnetic isolation plate 1623 are determined according to the actual detection needs, and will not be repeated here.
- the automatic sampling device 17 includes a reagent needle assembly 171 used for the injection operation of the reagent, a sampling needle assembly 172 and a gantry assembly 173 for the operation of the sample liquid injection.
- the needle assembly 171, the sampling needle assembly 172, and the gantry assembly 173 are all connected to the control system; the reagent needle assembly 171 and the sampling needle assembly 172 are respectively arranged on both sides of the gantry assembly 173, and the gantry assembly 173 can be moved along the basket 11, The consumable supply module and the reagent box loading module 14 move.
- the reagent needle assembly 171 is responsible for the extraction and filling of reagents
- the sample injection needle assembly 172 is responsible for adding and mixing the sample solution
- the gantry assembly 173 is responsible for transferring and positioning the reagent needle assembly 171 and the sample injection needle assembly 172 to realize multi-channel Synchronous operation, improve equipment detection efficiency.
- the basket 11 , the consumable supply module, and the reagent box loading module 14 are arranged side by side on the workbench at the upper end of the gantry assembly 173 .
- the control system controls the gantry assembly 173 to drive the sampling needle assembly 172 to move to the area where the extraction reagent strip 03 is loaded on the basket 11, and the sampling needle assembly 172 picks up the tip01 and punctures the extraction hole one by one.
- the gantry assembly 173 drives the reagent needle assembly 171 to move above the extraction reagent strip 03, the reagent needle assembly 171 fills the extraction reagent into the reaction chamber 031 of the extraction reagent strip 03, and adds
- the gantry assembly 173 drives the reagent needle assembly 171 to move to the top of the discarded hole, and the reagent needle assembly 171 exits tip01 and discards it; repeat the above steps to complete the sample addition process of all extracted reagents; the gantry assembly 173 drives the sample addition
- the needle assembly 172 extracts the sample liquid in the sample tube 04 loaded in the basket 11 and fills it into the reaction chamber 031 of the extraction reagent strip 03, and performs multiple injection operations on the mixed liquid in the reaction chamber 031 to make the extraction reagent Fully mixed with the sample solution; after the mixing is completed, the gantry assembly 173 drives the sample needle assembly 172 away from the extraction
- the control system controls the gantry assembly 173 to drive the sampling needle assembly 172 to extract all the liquid in the reaction chamber 031 and transfer it to the reagent hole of the extraction reagent strip 03; the gantry assembly 173 drives the sampling needle assembly 172 Extract the lotion in the extraction reagent strip 03 and fill it into the reaction chamber 031.
- the magnetic suction module 162 is far away from the reaction chamber 031.
- the lotion washes away the condensed magnetic beads on the inner wall of the reaction chamber 031;
- the sampling needle assembly 172 performs a plurality of pumping liquid operations, so that the magnetic beads and the washing liquid are fully mixed.
- the gantry assembly 173 drives the reagent needle assembly 171 to move above the reagent box loading module 14, extracts the amplification reagent and fills it into the PCR tube 021 located in the PCR consumable box 13, and discards it after filling is completed tip01; Repeat the above steps to complete the filling process of all amplification reagents.
- the magnetic attraction module 162 remains still and is still attached to the reaction chamber 031.
- the control system controls the gantry assembly 173 to drive the reagent needle assembly 171 to move above the area where the extraction reagent strip 03 is loaded on the basket 11, and extracts
- the purified product in the reaction chamber 031 is injected into the PCR tube 021 containing the amplification reagent; then the gantry assembly 173 drives the reagent needle assembly 171 to suck and extract the sealing liquid in the reagent strip 03, and injects it into the In the above PCR tube 021; finally, the gantry assembly 173 drives the reagent needle assembly 171 to move to the top of the PCR consumable box 13, pulls the sealing plug 022 and moves to the top of the PCR tube 021, and uses the sealing plug 022 to seal the PCR tube 021.
- the gantry assembly 173 includes a gantry and a sample loading and moving assembly that drives the gantry to move; the workbench is provided with a sample loading guide assembly that limits the moving direction of the gantry, and the sample loading guide assembly is slidably connected with the gantry, and the sample loading moving assembly Connect with the control system.
- the reagent needle assembly 171 and the sample injection needle assembly 172 are arranged on both sides of the gantry respectively, when the sample loading moving assembly drives the gantry to move along the extension direction of the sample loading guide assembly, the gantry drives the reagent needle assembly 171 and the sampling needle assembly 172 move along the basket 11 , the consumable supply module and the reagent box loading module 14 .
- the sample loading guide assembly can be set as a common linear displacement mechanism such as a guide rail slider mechanism, a guide sleeve guide column mechanism, or a rack and pinion.
- a common linear displacement mechanism such as a guide rail slider mechanism, a guide sleeve guide column mechanism, or a rack and pinion.
- the reagent needle assembly 171 includes a reagent needle, a pricking control assembly 1713, a pipetting control assembly, a reagent needle lifting assembly, and a reagent needle translation assembly 1714.
- the reagent needle includes an adapter 1711 and a reagent connected to the adapter 1711.
- the reagent pump 1712 is connected with the pipetting control assembly, and the adapter 1711 is connected with the pricking control assembly 1713, so that the pricking control assembly 1713 controls the adapter 1711 to prick or exit the tip01/PCR tube 021;
- the reagent needle translation assembly 1714 is used to drive The reagent needle moves along the vertical direction of the movement direction of the gantry assembly 173 in the horizontal plane, and the reagent needle lifting assembly is used to drive the reagent needle to move along the vertical direction.
- the reagent needle assembly 171 is provided with a reagent needle mounting seat, the reagent needle mounting seat is used to install the reagent needle, pricking control assembly 1713, pipetting control assembly and reagent needle lifting assembly, the reagent needle mounting seat
- the reagent needle translation component 1714 is slidably connected to the gantry component 173 .
- the adapter 1711 is used to cooperate with the tip01 or the sealing plug 022; the reagent pump 1712 is a suction pump used to connect and communicate with the adapter 1711, and is connected to the movement control assembly.
- the type and size of the adapter 1711 and the type, size, and position of the reagent pump 1712 are determined according to actual detection requirements with reference to the prior art, and will not be repeated here.
- the tying control assembly 1713 includes a guide post 1713-1 connected at one end to the sleeve mounting plate 1711-3, a guide post connecting plate 1713-2 connected to the other end of the guide post 1713-1 and
- the screw motor 1713-3 and the guide post connecting plate 1713-2 are arranged under the motor shaft of the screw motor 1713-3.
- the type, quantity, size and position of the guide pillars 1713-1 and the type, model and position of the screw motor 1713-3 are determined according to actual needs with reference to the prior art, and will not be repeated here.
- the pipetting pump 1721 includes a pump body and a plunger arranged in the pump body.
- the lower end of the pump body is used to cooperate with the tip01; the plunger is slidably connected with the pump body so that the air pressure in the pump body can be changed by changing the volume of the pump body to realize pumping and injection process.
- the quantity, type, model, etc. of the pipetting pump 1721 are determined according to actual needs with reference to the prior art, and will not be repeated here.
- the lifting assembly 1723 of the pipetting pump and the lifting assembly 1722 of the plunger include a linear displacement mechanism and a driving mechanism, wherein the linear displacement mechanism includes a guide rail slider mechanism, a synchronous belt mechanism, etc., and the driving mechanism includes a stepping motor, a screw motor, a hydraulic cylinder, an electric motor, etc. putter etc.
- the plunger lifting assembly 1722 is provided with a synchronous bar that engages with the plungers of the pipetting pumps 1721 .
- the gantry assembly 173 drives the sample needle assembly 172 to move along the X axis to the top of the disposable tip consumable box 12, and the sample needle lift assembly 1723 drives the pipetting pump 1721 to descend along the Z axis, so that tip01 is installed at the lower end of the pipetting pump 1721; after the pipetting pump 1721 and tip01 are matched, the lifting assembly 1723 drives the pipetting pump 1721 to rise along the Z axis, and the gantry assembly 173 drives the sampling needle assembly 172 to move along the X axis To the top of the reagent to be absorbed, the sampling needle assembly 1723 drives the pipetting pump 1721 to descend along the Z axis, and the plunger lifting assembly 172 drives the plunger to rise relative to the pump body along the Z axis to complete the pumping action; The lifting assembly 1723 drives the pipetting pump 1721 to rise along the Z axis, the gantry assembly 173 drives the sample needle assembly 172 to move to the position to
- the sampling needle assembly 172 further includes an aspiration liquid detection assembly 1724 for detecting the working state of the pipette pump 1721 and a Z-axis origin detection assembly 1725 for detecting whether the pipette pump lifting assembly 1723 is reset.
- the types, installation methods, positions, etc. of the aspiration liquid detection component 1724 and the Z-axis origin detection component 1725 are determined according to actual detection needs, and will not be described in detail here.
- the PCR transfer device 21 includes a handle assembly 211 for grasping or loosening the PCR tube 021, a cover opening assembly 212 for opening or closing the amplification cover 314 of the PCR amplification device 31,
- the X-axis assembly 213 for driving the handle assembly 211 and the cover-opening assembly 212 to move along the X-axis direction
- the Y-axis assembly 214 for driving the handle assembly 211 and the cover-opening assembly 212 to move along the Y-axis direction
- the Y-axis assembly 214 for driving the handle assembly 211 to move along the Y-axis direction.
- the shaft assembly 214 is slidingly connected to the X-axis assembly 213 .
- gripper assembly 211 and the cover opening assembly 212 are respectively slidably connected to the Z-axis assembly 215 , that is, there is no direct connection between them.
- the X-axis assembly 213, the Y-axis assembly 214 and the Z-axis assembly 215 all include a linear displacement mechanism and a drive mechanism.
- the linear displacement mechanism is one or a combination of linear displacement mechanisms such as a synchronous belt mechanism and a guide rail slider mechanism.
- Mechanisms include common driving mechanisms such as stepping motors, electric push rods, and hydraulic cylinders.
- the specific structures, dimensions, positions, connection methods, etc. of the X-axis assembly 213, the Y-axis assembly 214 and the Z-axis assembly 215 are determined according to actual testing needs, and will not be repeated here.
- the X-axis assembly 213 and the Y-axis assembly 214 drive the Z-axis assembly 215 to move directly above the amplification heating block 311 of the PCR amplification device 31; the Z-axis assembly 215 drives the opening assembly 212 along the Z-axis The downward movement makes the opening assembly 212 and the amplification cover 314 clamped and fixed; the Y-axis assembly 214 drives the handle assembly 211 and the opening assembly 212 to move along the Y-axis direction, so that the amplification cover 314 and the PCR amplification device 31 expand Increase the heat block 311 to separate, and complete the uncapping operation;
- the Z-axis assembly 215 drives the gripper assembly 211 to drop to the low position (grabbing position), and the cover opening assembly 212 rises to the high position; the X-axis assembly 213 and the Y-axis assembly 214 drive the gripper assembly 211 to move to the position of the PCR consumable box 13, and the gripper Component 211 grabs the PCR tube 021; X-axis component 213 and Y-axis component 214 drive the gripper component 211 to move to the amplification heating block 311 of the PCR amplification device 31, and the gripper component 211 releases the PCR tube 021 to make the PCR tube 021 Put into the amplification hole of the amplification heating block 311;
- the Z-axis assembly 215 drives the opening assembly 212 down to a low position, the handle assembly 211 rises to a high position, and the opening assembly 212 closes the amplification cover 314 .
- the PCR tube transfer device 21 effectively improves the grasping and transferring efficiency of the PCR tube 021, and ensures the accuracy of the grabbing and transferring operation of grabbing the PCR tube 021, and is simple in structure, convenient in operation, and easy to popularize .
- the PCR amplification device 31 includes an amplification heating block 311, an amplification heating sheet 312, an amplification radiator 313, an amplification cover 314 for covering the amplification heating block 311, and an amplification cover 314 for raising
- the amplification cooling assembly 315 of the heat dissipation efficiency of the amplification radiator 313, the amplification heating block 311 is provided with at least two amplification holes for placing the PCR tube 021, the amplification cover 314, the amplification heating block 311, the amplification heating
- the sheet 312 and the amplification radiator 313 are arranged sequentially from top to bottom.
- the amplification heating block 311 is equipped with an amplification temperature sensor for detecting the temperature of the amplification heating block 311 in real time, so that the inspector can grasp the temperature of the amplification heating block 311 in real time, so as to effectively control the heating temperature.
- the PCR amplification device 31 works, first open the amplification cover 314, put the PCR tube 021 containing the nucleic acid sample into the amplification hole of the amplification heating block 311; after the PCR tube 021 is placed, cover the amplification hole. cover 314, and use the amplification heating plate 312 to heat the amplification heating block 311, so as to perform PCR amplification operation on the nucleic acid sample.
- the amplification cover 314 includes a frame and a top cover that can slide laterally along the frame, the top cover and the amplification heating block 311 are arranged in one-to-one correspondence, the frame is provided with a cover opening sensor for detecting whether the top cover is opened, and the top cover is provided with a for the opening flap that triggers the opening sensor.
- the structure, material, size and position of the frame, top cover, cover opening sensor and cover opening stopper are determined according to actual needs, and will not be repeated here.
- the position of the opening stopper corresponds to the position of the opening sensor, and the opening stopper triggers the cover opening sensor; when the cover is opened, the opening stopper slides with the top cover, and the opening stopper and the cover opening sensor are separated , the opening flap will not trigger the opening sensor.
- the fluorescence detection device 32 includes a light source assembly 321 for providing a fluorescence light source, a fluorescence detection assembly 322 for quantitative detection, a light source optical fiber 323, and a fluorescence detection optical fiber 324.
- One side of the amplification hole is connected to the One end of the light source fiber 323 is connected, the other side of the amplification hole is connected with one end of the fluorescence detection fiber 324;
- the light source optical fiber 323 and the fluorescence detection optical fiber 324 are respectively connected to both sides of the amplification heating block 311, and the angle between the light source optical fiber 323 and the fluorescence detection optical fiber 324 is 90°, so that each optical fiber can be positioned accurately and firmly, which is beneficial to the measurement The accuracy of the light value.
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Abstract
一种DNA、RNA核酸共提取及检测系统，包括核酸提取系统、封口转运混匀系统、核酸检测系统和控制系统，核酸提取系统、封口转运混匀系统和核酸检测系统均与控制系统连接；核酸提取系统包括用于装载提取试剂条和样本管的提篮、耗材供给模块、试剂盒载入模块、用于自动录入条码信息的条码扫描装置、磁吸温育装置以及用于向提取试剂条的反应仓或PCR管内移液并密封PCR管的自动加样装置；封口转运系统包括用于抓取并带动PCR管移动的PCR转移装置以及高速混匀装置；核酸检测系统包括PCR扩增装置和荧光检测装置。
Description
本申请要求于2021年05月13日提交中国专利局、申请号为202110523722.5、发明名称为“一种DNA、RNA核酸共提取及检测系统”的中国专利申请的优先权，其全部内容通过引用结合在本申请中。
本发明涉及PCR技术领域，更具体地说，涉及一种DNA、RNA核酸共提取及检测系统。
由于分子诊断灵敏度高、特异性高、方便快捷的特点，广泛应用于临床诊断。
现有的核酸提取扩增系统多采用批量进样方式、检测项目少，且待测样本的温育、磁吸和转移等过程多为人工操作，操作过程中样本供给、试剂条供给和耗材废弃多采用人工完成，检测人员工作强度大、易产生疲劳，且检测效率低、检测成本较高。
同时，各检测工序需在独立操作空间内分别完成，检测人员需要在多个检测室内穿梭检测，检测效率低。
综上所述，如何提高核酸提取扩增效率，是目前本领域技术人员亟待解决的问题。
发明内容
有鉴于此，本发明的目的是提供一种DNA、RNA核酸共提取及检测系统，实现了提取扩增过程中的条码扫描、加样、温育提取、转移混匀和检测的自动化，有效地提高了核酸提取扩增效率。
为了实现上述目的，本发明提供如下技术方案：
一种DNA、RNA核酸共提取及检测系统，包括核酸提取系统、封口转运混匀系统、核酸检测系统和控制系统，所述核酸提取系统、所述封口转运混匀系统和所述核酸检测系统均与控制系统连接；
所述核酸提取系统包括用于装载提取试剂条和样本管的提篮、耗材供 给模块、试剂盒载入模块、用于自动录入条码信息的条码扫描装置、磁吸温育装置以及用于向所述提取试剂条的反应仓或PCR管内移液并密封所述PCR管的自动加样装置；
所述封口转运系统包括用于抓取并带动所述PCR管移动的PCR转移装置以及高速混匀装置；
所述核酸检测系统包括PCR扩增装置和荧光检测装置。
优选的，所述提篮包括提篮盖板、用于盛放提取试剂条的提篮底板以及用于盛放样本管的样本座，所述样本座与所述提篮底板可拆卸连接；
所述提篮盖板与所述提篮底板可转动连接，以便在所述提篮盖板与所述提篮底板贴合时限制所述提取试剂条在高度方向上的位移。
优选的，所述条码扫描装置包括安装支架、扫描组件、用于在扫码位和待机位之间推动提取试剂条的推手组件、带动所述推手组件沿所述安装支架的长度方向运动的推手移动组件以及检测组件，所述检测组件用于判断当前通道是否存在提取试剂条和样本管，所述检测组件与所述推手移动组件信号连接，所述扫描组件、所述检测组件均安装于所述推手组件的安装底板上。
优选的，所述磁吸温育装置包括用于加热反应仓的温育模块、用于对所述反应仓进行磁吸的磁吸模块以及驱动组件，所述温育模块、所述磁吸模块均与所述驱动组件连接，所述驱动组件用于驱动所述温育模块和所述磁吸模块交替靠近所述反应仓。
优选的，所述温育模块包括设有试剂槽的加热块、贴合所述试剂槽的内壁面设置的加热组件、设置于所述加热块底部的散热组件以及用于检测所述加热块温度的温度传感器，所述温度传感器与所述加热块连接。
优选的，所述磁吸模块包括磁铁以及用于驱动所述磁铁转动的旋转组件，所述旋转组件与所述磁铁连接；
所述磁铁包括第一磁吸面和第二磁吸面，所述第一磁吸面和所述第二磁吸面二者面积大小不同且均用于吸附反应仓内的磁珠。
优选的，所述PCR转移装置包括用于抓取或松开PCR管的抓手组件、用于打开或关闭所述PCR扩增装置的扩增盖的开盖组件、用于驱动所述抓手组件与所述开盖组件沿X轴方向移动的X轴组件、用于驱动所述抓手组件与所述开盖组件沿Y轴方向移动的Y轴组件以及用于驱动所述抓手组件与所述开盖组件沿Z轴方向移动的Z轴组件；
所述抓手组件和所述开盖组件分别滑动连接于所述Z轴组件，所述Z轴组件滑动连接于所述Y轴组件，所述Y轴组件滑动连接于所述X轴组 件。
优选的，所述抓手组件包括抓手底板、平行设于所述抓手底板下方的抓手活动板、用于抓取PCR管的抓手以及用于控制所述抓手张开或收缩的收缩驱动组件，所述收缩驱动组件设于所述抓手底板上，所述收缩驱动组件的伸缩端穿过所述抓手底板与所述抓手活动板连接、以驱动所述抓手活动板上升或下降；
所述抓手底板和所述抓手活动板均与所述收缩驱动组件连接，当所述抓手活动板下降时所述抓手张开，当所述抓手活动板上升时所述抓手收缩。
优选的，所述PCR扩增装置包括扩增加热块、扩增加热片、扩增散热器、用于盖合所述扩增加热块的扩增盖以及用于提高所述扩增散热器的散热效率的扩增冷却组件，所述扩增加热块设有至少两个用于放置PCR管的扩增孔，所述扩增盖、所述扩增加热块、所述扩增加热片以及所述扩增散热器由上至下依次设置。
优选的，所述荧光检测装置包括用于提供荧光光源的光源组件、用于进行定量检测的荧光检测组件、光源光纤以及荧光检测光纤，所述扩增孔的一侧与所述光源光纤的一端连接，所述扩增孔的另一侧与所述荧光检测光纤的一端连接；
所述光源光纤的另一端与所述光源组件连接，所述荧光检测光纤的另一端与所述荧光检测组件连接。
优选的，所述自动加样装置包括用于试剂的抽注液操作的试剂针组件、用于样本液的抽注液操作的加样针组件以及龙门架组件，所述试剂针组件、所述加样针组件、所述龙门架组件均与所述控制系统连接；
所述试剂针组件和所述加样针组件分别设置于所述龙门架组件的两侧，所述龙门架组件可沿所述提篮、所述耗材供给模块以及所述试剂盒载入模块运动。
优选的，所述试剂针组件包括试剂针、扎取控制组件、移液控制组件、试剂针升降组件以及试剂针平移组件，所述试剂针包括适配器以及与所述适配器连通的试剂泵，所述试剂泵与移液控制组件连接，所述适配器与所述扎取控制组件连接，以便所述扎取控制组件控制所述适配器扎取或退出tip或密封塞；
所述试剂针平移组件用于驱动所述试剂针沿水平面内、所述龙门架组件运动方向的垂直方向运动，所述试剂针升降组件用于驱动所述试剂针沿竖直方向运动。
优选的，所述加样针组件包括若干个并列设置的移液泵、用于带动所 述移液泵升降的移液泵升降组件以及用于驱动所述移液泵的柱塞相对所述移液泵的泵体滑动的柱塞升降组件，所述移液泵安装于所述移液泵升降组件，所述柱塞升降组件与所述移液泵的柱塞连接。
本发明提供的DNA、RNA核酸共提取及检测系统工作时，首先在提篮处装载盛放待测样本的样本管和提取试剂条，利用条码扫描装置录入样本管和提取试剂条的条码信息；录入完成后，自动加样装置刺破提取试剂条的封膜，并向提取试剂条的反应仓内加注提取试剂和待测样本；磁吸温育装置对待测样本进行温育磁吸处理，提取待测样本内的核酸；自动加样装置向PCR管内加注扩增试剂和温育磁吸后的提纯物，并利用密封塞密封PCR管；密封后，PCR转移装置抓取PCR管并将其转移至高速混匀装置处；混匀结束后，PCR转移装置将PCR管转移至PCR扩增装置内，以完成PCR扩增过程；扩增过程结束后，荧光检测装置对PCR管进行荧光检测。
因此，本发明提供的DNA、RNA共提取及扩增系统实现了检测过程中条码扫描、加样、温育提取、转移混匀和荧光检测的自动化，有效地提高了核酸提取扩增效率。
为了更清楚地说明本发明实施例或现有技术中的技术方案，下面将对实施例或现有技术描述中所需要使用的附图作简单地介绍，显而易见地，下面描述中的附图仅仅是本发明的实施例，对于本领域普通技术人员来讲，在不付出创造性劳动的前提下，还可以根据提供的附图获得其他的附图。
图1为本发明所提供的DNA、RNA核酸共提取及检测系统的具体实施例的结构示意图；图2为提篮的结构示意图；图3为提篮的爆炸示意图；图4为工作台上卡扣组件的结构示意图；图5为工作台上压紧组件的结构示意图；图6为一次性耗材tip盒的结构示意图；图7为PCR耗材盒的结构示意图；图8为条码扫描装置的结构示意图；图9为的扫描组件结构示意图；图10为推手组件的结构示意图；图11为磁吸温育装置的结构示意图；图12为温育模块的结构示意图；图13为图12的剖视示意图；图14为磁吸模块的结构示意图；图15为磁铁、磁铁支架和隔磁板的装配示意图；图16为磁铁的结构示意图；图17为自动加样装置的结构示意图；图18为自动加样装置在另一方向上的结构示意图；图19为试剂针组件的结构示意图；图20为加样针组件的结构示意图；图21为PCR转移装置的结构示意图；图22为Z轴组件的结构示意图；图23为抓手组件的结构示意图； 图24为PCR扩增装置的爆炸示意图；图25为荧光检测装置的结构示意图。
图1-图25中：
01为tip；021为PCR管、022为密封塞；03为提取试剂条、031为反应仓；04为样本管；11为提篮、111为提篮底板、112为提篮盖板、113为样本座、114为支撑组件、115为提手、116为提篮定位组件；12为一次性tip耗材盒；13为PCR耗材盒；14为试剂盒载入模块；15为条码扫描装置、151为安装支架、152为推手移动组件、153为扫描组件、1531为反射镜、1532为读码器、154为推手组件、155为检测组件；16为磁吸温育装置、161为温育模块、162为磁吸模块、163为驱动组件、164为限位支撑组件、1611为加热块、1611-1为试剂槽、1612为加热组件、1613为散热组件、1614为温度传感器、1615为保温组件、1616为冷却组件、1621为磁铁、1621-1为第一磁吸面、1621-2为第二磁吸面、1622为旋转组件、1623为隔磁板；17为自动加样装置、171为试剂针组件、172为加样针组件、173为龙门架组件、1711为适配器、1711-1为套筒、1711-2为适配器安装板、1711-3为套筒安装板、1712为试剂泵、1713为扎取控制组件、1713-1为导柱、1713-2为导柱连接板、1713-3为丝杆电机、1714为试剂针平移组件、1715为取tip检测组件、1716为退tip检测组件、1721为移液泵、1722为柱塞升降组件、1723为移液泵升降组件、1724为抽注液检测组件、1725为Z轴原点检测组件；18为兜液抽风装置、191为卡扣组件、192为压紧组件；21为PCR转移装置、211为抓手组件、212为开盖组件、213为X轴组件、214为Y轴组件、215为Z轴组件、22为高速混匀装置；31为PCR扩增装置、311为扩增加热块、312为扩增加热片、313为扩增散热器、314为扩增盖、315为扩增冷却组件、32为荧光检测装置、321为光源组件、322为荧光检测组件、323为光源光纤、324为荧光检测光纤。
下面将结合本发明实施例中的附图，对本发明实施例中的技术方案进行清楚、完整地描述，显然，所描述的实施例仅仅是本发明一部分实施例，而不是全部的实施例。基于本发明中的实施例，本领域普通技术人员在没 有做出创造性劳动前提下所获得的所有其他实施例，都属于本发明保护的范围。
本发明的核心是提供一种DNA、RNA核酸共提取及检测系统，实现了提取扩增过程中的条码扫描、加样、温育提取、转移混匀和检测的自动化，有效地提高了核酸提取扩增效率。
请参考图1-图25。需要进行说明的是，本申请文件中X轴方向为自动加样装置17的龙门架组件173的运动方向，Y轴方向为水平面内与X轴方向的垂直的方向，Z轴方向为竖直方向。
本发明提供的一种DNA、RNA核酸共提取及检测系统，包括核酸提取系统、封口转运混匀系统和核酸检测系统；核酸提取系统包括用于装载提取试剂条03和样本管04的提篮11、耗材供给模块、试剂盒载入模块14、用于自动录入条码信息的条码扫描装置15、磁吸温育装置16以及用于向提取试剂条03的反应仓031或PCR管021内移液并密封PCR管021的自动加样装置17；封口转运系统包括用于抓取并带动PCR管021移动的PCR转移装置21以及高速混匀装置22；核酸检测系统包括PCR扩增装置31和荧光检测装置32。
其中，耗材供给模块用于盛放检测过程中所需的一次性耗材，如tip01和PCR管021，检测过程中耗材通过自动加样装置17和PCR转移装置21取放，无需检测人员手动操作，有效地减轻了检测人员的工作强度。
请参考图1，耗材供给模块包括用于盛放tip01的一次性tip耗材盒12和用于盛放PCR管021以及其对应的密封塞022的PCR耗材盒13。一次性tip耗材盒12和PCR耗材盒13的结构、材质、尺寸和位置等根据实际检测需要确定。
试剂盒载入模块14用于放置PCR体系构建所需的多种扩增试剂，试剂盒载入模块14的结构、材质、尺寸和位置等根据实际检测需要确定。
对待测样本进行检测前，在提篮11处装载盛放待测样本的样本管04和提取试剂条03，并利用条码扫描装置15录入样本管04和提取试剂条03的条码信息；检测时，将提篮11固定于工作台上，自动加样装置17刺破提取试剂条03的封膜，并向提取试剂条03的反应仓031内逐步加注提取 试剂和待测样本的样本液；加样完成后，磁吸温育装置16对混合液进行温育磁吸处理，提取混合液内的核酸；提取完成后，自动加样装置17向PCR管021内逐步加注扩增试剂和温育磁吸后的提纯物，并在移液完成后利用密封塞022密封PCR管021；密封完成后，PCR转移装置21抓取PCR管021并将其转移至高速混匀装置22处，进行高速混匀；混匀结束后，PCR转移装置21将PCR管021转移至PCR扩增装置31内，以完成PCR扩增过程；扩增过程结束后，荧光检测装置32对PCR管021进行荧光检测。
因此，本实施例提供的DNA、RNA共提取及扩增系统实现了检测过程中条码扫描、加样、温育提取、转移混匀和荧光检测的自动化，有效地提高了核酸提取扩增效率。
优选的，核酸提取系统还设有兜液抽风装置18，兜液抽风装置18包括用于收集移液中滴落试剂的兜液盒和对抽注液位进行抽风处理的抽风装置。兜液盒能够有效防止试剂滴落在操作平面致使交叉污染；抽风装置能够将抽注液产生的污染气体抽出，避免气溶胶扩散引起的污染。
在上述实施例的基础上，提篮11包括提篮盖板112、用于盛放提取试剂条03的提篮底板111以及用于盛放样本管04的样本座113，样本座113与提篮底板111可拆卸连接；提篮盖板112与提篮底板111可转动连接，以便在提篮盖板112与提篮底板111贴合时限制提取试剂条03在高度方向上的位移。
请参考图2，提篮底板111中央设有若干个沿其长度方向平行设置的试剂条安装孔，样本座113上设有若干个样本管安装孔；试剂条安装孔和样本管安装孔的尺寸和数量根据实际检测需要确定。
提篮盖板112与提篮底板111可转动连接，以防止提取试剂条03在提篮11的高度方向上窜动，优选的，提篮盖板112可转动地连接于提篮底板111长度方向的一端；提篮盖板112上设有与试剂条安装孔一一对应的试剂条定位孔。
优选的，提篮底板111的顶面设有第一磁铁，提篮盖板112的底板设有第二磁铁，当提篮盖板112与提篮底板111贴合时，第一磁铁与第二磁铁吸合。因此，提取试剂条03翘起或脱出时需要克服第一磁铁与第二磁铁 之间的吸力，实现了对提取试剂条03在提篮11高度方向上的有效限位和准确定位。此外，第一磁铁和第二磁铁也可以替换为相互卡接的定位槽和定位块等类似的定位结构。
为了方便提取试剂条03和样本管04的装载，优选的，在提篮底板111的底面设有支撑组件114，支撑组件114具体可以是图2所示的工字型结构，也可以是设置于提篮底板111四角处的支撑柱，还可以设置为其他常见的支架结构。
装载提取试剂条03和样本管04时，旋转提篮盖板112，在提篮底板111和样本座113内分别放置提取试剂条03和样本管04，放置完成后旋转提篮盖板112使提篮底板111与其贴合；装载完成后，将提篮11置入工作台上并固定；检测完成后，将提篮11自工作台上取下，废弃使用过的提取试剂条03和样本管04。
在本实施例中，实现了对提取试剂条03和样本管04的批量同步装载，节省了多次放置的繁琐过程，放置效率高；提篮11可对放入的提取试剂条03和样本管04进行准确定位，避免了提篮11置于工作台后对提取试剂条03和样本管04重新定位。
为了固定提篮11，工作台上设有用于定位提篮11的卡扣组件191和压紧组件192，卡扣组件191和压紧组件192分别与提篮11长度方向上的两端卡接，卡扣组件191和压紧组件192的结构、尺寸、材质、数量和设置位置等请根据实际生产需要确定，在此不再赘述。
优选的，为了方便提篮11的转运、提高转运效率，提篮底板111与提手115可转动连接，提手115的结构、形状、尺寸、数量、材质和位置等根据实际需要确定。
优选的，提篮11还包括提篮定位组件116，提篮定位组件116包括导向座、拉伸弹簧、沿提篮底板111的长度方向设置的导向轴和用于对提取试剂条03进行限位的提篮挡板，导向座连接于提篮底板111的底面；导向轴与导向座滑动连接，导向轴的一端与提篮挡板垂直连接，拉伸弹簧的两端分别与提篮挡板、提篮底板111的底面连接。
请参考图3，以靠近提篮底板111与提篮盖板112的转轴的一端为提 篮底板111的前端。载入提取试剂条03时，人工拉动拉伸弹簧带动提篮挡板向提篮底板111的前端运动，提篮挡板位于提取试剂条03的右侧，因此提篮挡板推动提取试剂条03向提篮底板111的前端运动，直至提取试剂条03与试剂条安装孔的左壁面接触，此时各提取试剂条03的放置位置一致。
在上述实施例的基础上，条码扫描装置15包括安装支架151、扫描组件153、用于在扫码位和待机位之间推动提取试剂条03的推手组件154、带动推手组件154沿安装支架151的长度方向运动的推手移动组件152以及检测组件155，检测组件155用于判断当前通道是否存在提取试剂条03和样本管04，检测组件155与推手移动组件152信号连接，扫描组件153、检测组件155均安装于推手组件154的安装底板上。
推手移动组件152包括直线位移机构和驱动机构，直线位移机构沿安装支架151的长度方向设置，具体可以设置为同步带机构、导轨滑块机构等常见的直线位移结构与步进电机、电动推杆、液压缸等驱动机构的组合。
请参考图9，扫描组件153包括反射镜1531和读码器1532，反射镜1531用于反射读码器1532的检测光线至样本管04的条码，以实现读码器1532对样本管04的扫码录入。读码器1532的种类和尺寸、反射镜1531的种类和尺寸以及反射镜1531与读码器1532的相对位置关系等请根据实际需要参考现有技术确定，在此不再赘述。
推手组件154包括直线位移机构、驱动机构以及推动提取试剂条03运动的推手，推手连接于直线位移机构；直线位移机构和驱动机构的具体设置方案请参考前述推手移动组件152。
优选的，请参考图10，推手组件154的安装底板设有用于与提取试剂条03卡接的推块、线轨、同步带机构以及驱动同步带机构的步进电机，推块与线轨滑动连接，线轨的长度方向与安装支架151的长度方向垂直。步进电机转动时，驱动同步带机构带动推块相对线轨滑动，而推块与提取试剂条03接触，使得提取试剂条03在待机位和扫码位之间运动。
检测组件155包括用于检测当前通道内是否存在提取试剂条03的试剂条检测传感器和用于检测当前通道内是否存在样本管04的样本管检测传感器，试剂条检测传感器与推手组件154信号连接。
当试剂条检测传感器检测到提取试剂条03时，推手组件154带动提取试剂条03由待机位向扫码位移动；样本管检测传感器检测到样本管04时，扫描组件153对样本管04的条码信息进行扫描；二者均未检测到条码信息时，推手移动组件152带动推手组件154移动至下一通道内。
工作时，首先由检测组件155判断当前通道内是否存在提取试剂条03和样本管04，若是则由推手组件154带动提取试剂条03运动至扫码位、由扫描组件153对提取试剂条03和/或样本管04的二维码进行扫描，扫描完成后，推手组件154带动提取试剂条03返回待机位，推手移动组件152带动推手组件154及扫描组件153运动至下一通道内；若否，则推手移动组件152直接带动推手组件154和扫描组件153移动至下一通道内。
在本实施例中，扫描组件153和推手组件154可沿安装支架151的长度方向移动，能够读取多通道的条码信息；扫描组件153既可对提取试剂条03进行扫描，也可对样本管04进行扫描，检测速度快；同时，条码扫描装置15结构紧凑，可在狭长空间内运行，有利于减小DNA、RNA核酸共提取及检测系统的机台尺寸、提高空间利用率。
优选的，检测组件155还包括用于检测提取试剂条03是否到达扫码位的推手到位传感器和用于检测提取试剂条03是否重回待机位的推手复位传感器，推手到位传感器和推手复位传感器均与推手组件154信号连接。推手到位传感器和推手到位传感器均可设置为接近开关、行程开关等。
推手组件154运动至安装支架151的最后通道时，可以控制推手移动组件152带动推手组件154复位，自第一通道依次对各通道内的提取试剂条03和样本管04进行检测；也可以控制推手移动组件152带动扫描组件153对下一组待检测的提取试剂条03和样本管04进行倒序扫描。
优选的，为了实现扫描组件153的复位，检测组件155还可以包括用于检测扫描组件153是否运动至安装支架151末端的扫描复位传感器，扫描复位传感器与推手移动组件152信号连接。
在上述实施例的基础上，磁吸温育装置16包括用于加热反应仓031的温育模块161、用于对反应仓031进行磁吸的磁吸模块162以及驱动组件163，温育模块161、磁吸模块162均与驱动组件163连接，驱动组件 163用于驱动温育模块161和磁吸模块162交替靠近反应仓031。
自动加样装置17向反应仓031加入各提取试剂和待测样本的样本液后，控制驱动组件163使温育模块161靠近反应仓031，以对反应仓031内的混合液进行有效加热、方便对混合液进行温育操作，同时磁吸模块162远离反应仓031避免对温育过程产生影响；温育过程完成后，控制驱动组件163使磁吸模块162靠近反应仓031，使混合液内的磁珠被吸附至反应仓031的内壁面，同时温育模块161远离反应仓031；磁吸操作完成后，控制驱动组件163使磁吸模块162远离反应仓031。
在本实施例中，利用驱动组件163交替控制温育模块161和磁吸模块162靠近反应仓031，实现了温育模块161与反应仓031的接触和分离，避免了温育模块161一直加热反应仓031内的混合液致使混合液温度升降速度过慢，提高了装置运行效率；同时，温育模块161和磁吸模块162的一体化设置，有效地减小了装置的整体尺寸，简化了温育和磁吸操作步骤。
驱动组件163可设置为丝杠驱动机构、导轨驱动机构等多种驱动机构，优选的，驱动组件163为丝杠驱动机构，丝杠驱动机构可如图11所示竖直设置，也可以根据温育磁吸装置16的整体布局水平设置，只要驱动组件163可带动温育模块161升降即可。
优选的，磁吸温育装置16还可以包括限位支撑组件164，磁吸模块162和驱动组件163均与限位支撑组件164连接，限位支撑组件164设有用于限制磁吸模块162的横移轨迹的限位轨道；当驱动组件163驱动温育模块161上升时，磁吸模块162沿限位轨道远离反应仓031，当驱动组件163驱动温育模块161下降时，磁吸模块162沿限位轨道靠近反应仓031。
请参考图11-图14，优选的，限位支撑组件164包括水平支撑板和竖直支撑板，水平支撑板和竖直支撑板垂直连接；水平支撑板上设有与限位轨道，磁吸模块162与限位轨道滑动连接；竖直支撑板顶部沿竖直方向设有与磁吸模块162连接的同步带机构；同步带机构上设有挡片，驱动组件163的伸缩端设有用于与挡片抵接的动力片。
当驱动组件163的伸缩端带动温育模块161下降时，动力片与挡片卡接，使得动力片可带动挡片向下移动，进而同步带机构的同步带向下移动 并将其拉紧，使得磁吸模块162沿着限位轨道靠近竖直支撑板并靠近贴合反应仓031；反之，当驱动组件163的伸缩端带动温育模块161上升时，动力片向上运动并与挡片分离，带动磁吸模块162远离反应仓031；因此实现了温育模块161的升降运动与磁吸模块162的靠近或远离反应仓031的运动通过同一驱动组件163控制，简化了磁吸温育装置16的整体结构。
为了方便磁吸模块162复位，可以在水平支撑板上设置磁吸复位弹簧，磁吸复位弹簧的两端分别与竖直支撑板、磁吸模块162连接；或设置驱动磁吸模块162沿限位组件移动的磁吸移动组件，磁吸模块162安装于磁吸移动组件，磁吸移动组件具体可包括直线导轨机构等直线位移机构和丝杠电机、液压缸等驱动机构。
在上述实施例的基础上，温育模块161包括设有试剂槽1611-1的加热块1611、贴合试剂槽1611-1的内壁面设置的加热组件1612、设置于加热块1611底部的散热组件1613以及用于检测加热块1611温度的温度传感器1614，温度传感器1614与加热块1611连接。
为了实现对多个反应仓031的同时温育，加热块1611内设有若干个试剂槽1611-1，使得加热时多个反应仓031温育温度相同，解决了温度均一性问题，有利于提高检测结果的准确性、减少实验误差。试剂槽1611-1的具体数量根据实际需要确定，在此不再赘述。
优选的，加热组件1612的加热功率在试剂槽1611-1的内壁面上的分布为非均匀性分布，以保证加热块1611内部各个位置的温度均匀性。
优选的，加热块1611的外周部设置保温组件1615，并在散热组件1613下方设置冷却组件1616，以提高散热效率、方便温育温度在温育温度范围内的任意快速切换。
加热块1611、加热组件1612、散热组件1613、温度传感器1614、保温组件1615以及冷却组件1616的结构、材质、尺寸、位置和连接方式等根据实际温育需要参考现有技术确定，在此不再赘述。
温育模块161工作时，首先加热组件1612对加热块1611进行加热，当温度传感器1614检测到加热块1611的温度到达第一预设温度(通常设置为37℃)时，停止加热组件1612的加热操作，保温组件1615保持加热 块1611温度恒定，以便对反应仓031内样本液进行温育。
当首次温育结束并完成磁吸后，再次利用加热组件1612迅速加热该加热块1611，当温度传感器1614检测到加热块1611的温度到达第二预设温度(通常设置为80℃)时，停止加热组件1612的加热操作，开启保温组件1615保持加热块1611温度恒定，以便对样本液再次温育。
当样本液温育结束后，利用散热组件1613对加热块1611进行降温，同时开启冷却组件1616以提高散热组件1613的散热效率。
在本实施例中，加热组件1612贴合试剂槽1611-1的内壁面设置，使得试剂槽1611-1温度的迅速增加；冷却组件1616加快了散热组件1613的散热效率，使得试剂槽1611-1的温度迅速下降，从而实现了温育温度的迅速转变，提高了温育效率。
在上述实施例的基础上，磁吸模块162包括磁铁1621以及用于驱动磁铁1621转动的旋转组件1622，旋转组件1622与磁铁1621连接；磁铁1621包括第一磁吸面1621-1和第二磁吸面1621-2，第一磁吸面1621-1和第二磁吸面1621-2二者面积大小不同且均用于吸附反应仓031内的磁珠。
多个磁铁1621相互平行设置，磁铁1621的数量与温育模块161内试剂槽1611-1的数量相同；第一磁吸面1621-1和第二磁吸面1621-2二者磁吸面面积不同，以便通过切换第一磁吸面1621-1和第二磁吸面1621-2改变磁吸模块162的磁力大小。
请参考图17-18，第一磁吸面1621-1的面积大于第二磁吸面1621-2的面积，因此当反应仓031需要较大磁力时，控制旋转组件1622将第一磁吸面1621-1转动至朝向反应仓031的配合面；反之，当需要较小磁力时，控制旋转组件1622将第二磁吸面1621-2转动至朝向反应仓031的配合面。
磁吸模块162工作时，依据所需的磁力大小确定朝向反应仓031的磁吸面，控制旋转组件1622使上述磁吸面朝向反应仓031的配合面。当需要更改磁吸面时，控制磁吸模块162远离反应仓031，而后控制旋转组件1622转动使另一磁吸面对转反应仓031，调整完成后控制磁吸模块162靠近反应仓031并使另一磁吸面与反应仓031的配合面贴合。
在本实施例中，旋转组件1622通过磁吸面的转换实现了磁力的变换， 且结构简单、操作方便、便于推广。
优选的，为了避免非磁吸状态下磁铁1621对反应仓031的磁珠混悬液的干扰，磁吸模块162设有用于在非磁吸过程中对磁珠进行隔磁的隔磁板1623，隔磁板1623的形状、尺寸、材质、连接方式、连接位置等等根据实际检测需要确定，在此不再赘述。
优选的，旋转组件1622包括用于驱动磁吸模块162的旋转轴的同步带机构以及驱动同步带机构的旋转电机。旋转电机、同步带机构以及旋转轴的形状、结构、尺寸、材质、位置等根据实际检测需要确定，在此不再赘述。旋转电机工作时，带动同步带机构转动，进而带动磁吸模块162的旋转轴转动，当旋转轴转动时磁铁1621周向转动、切换第一磁吸面1621-1、第二磁吸面1621-2以及隔磁板1623，进而实现磁力切换和隔磁操作。
此外，也可以直接利用旋转电机驱动旋转轴。
在上述实施例的基础上，自动加样装置17包括用于试剂的抽注液操作的试剂针组件171、用于样本液的抽注液操作的加样针组件172以及龙门架组件173，试剂针组件171、加样针组件172、龙门架组件173均与控制系统连接；试剂针组件171和加样针组件172分别设置于龙门架组件173的两侧，龙门架组件173可沿提篮11、耗材供给模块以及试剂盒载入模块14运动。
其中，试剂针组件171负责试剂的提取和加注，加样针组件172负责样本液的添加和混匀，龙门架组件173负责转移定位试剂针组件171和加样针组件172，以实现多通道同步操作、提高设备检测效率。
请参考图17，提篮11、耗材供给模块、试剂盒载入模块14并列设置于龙门架组件173上端的工作台上。
自动加样装置17工作时，首先，控制系统控制龙门架组件173驱动加样针组件172移动至提篮11装载提取试剂条03的区域，加样针组件172扎取tip01并逐个孔位刺破提取试剂条03顶部的铝塑膜；龙门架组件173驱动试剂针组件171移动至一次性tip耗材盒12上方、试剂针组件171扎取tip01，龙门架组件173驱动试剂针组件171移动至试剂盒载入模块14上方、试剂针组件171抽取提取试剂，龙门架组件173驱动试剂针组件171 移动至提取试剂条03上方、试剂针组件171向提取试剂条03的反应仓031内加注提取试剂，加注完成后龙门架组件173驱动试剂针组件171移动至废弃孔位上方、试剂针组件171退出tip01并将其废弃；重复上述步骤，完成全部提取试剂的加样过程；龙门架组件173驱动加样针组件172提取提篮11装载的样本管04中的样本液并加注至提取试剂条03的反应仓031中，并对反应仓031内的混合液进行多次抽注液操作，以使提取试剂和样本液充分混合；混合完成后，龙门架组件173驱动加样针组件172远离提取试剂条03，以方便后续的温育磁吸过程。
磁吸过程中，控制系统控制龙门架组件173驱动加样针组件172抽取反应仓031内的全部液体并将其转移至提取试剂条03的试剂孔中；龙门架组件173驱动加样针组件172抽取提取试剂条03内的洗液加注到反应仓031内，此时磁吸模块162远离反应仓031，加注过程中洗液将反应仓031内壁面上凝聚的磁珠冲开；加注完成后，加样针组件172进行多个抽注液操作，以便磁珠和洗液充分混合。
磁吸温育过程中，由于试剂针组件171处于空闲期，为了加快检测速度、减少检测时间，优选地利用试剂针组件171进行PCR体系的构建。首先，龙门架组件173驱动试剂针组件171移动至试剂盒载入模块14上方，抽取扩增试剂并将其加注至位于PCR耗材盒13内的PCR管021中，并在加注完成后废弃tip01；重复上述步骤，完成全部扩增试剂的加注过程。
温育磁吸过程结束后，磁吸模块162保持不动、仍与反应仓031贴合，控制系统控制龙门架组件173驱动试剂针组件171移动至提篮11装载提取试剂条03的区域上方，抽取反应仓031内的提纯物并将其加注入盛放有扩增试剂的PCR管021内；而后龙门架组件173驱动试剂针组件171吸取提取试剂条03内的密封液，并将其加注到上述PCR管021中；最后，龙门架组件173驱动试剂针组件171运动至PCR耗材盒13上方，扎取密封塞022后移动至PCR管021上方，利用密封塞022密封PCR管021。
需要进行说明的是，在上述过程中，试剂针组件171和加样针组件172吸取或加注的液体种类改变时，需要废弃更换之前使用的tip01，以免移液过程中发生试剂污染。
在本实施例中，实现了样本液和试剂的全自动提取和添加，有效地降低了检测人员劳动强度，并提高了检测效率和操作准确性。
优选的，龙门架组件173包括龙门架和驱动龙门架移动的加样移动组件；工作台设有限制龙门架移动方向的加样导向组件，加样导向组件与龙门架滑动连接，加样移动组件与控制系统连接。
请参考图17，试剂针组件171和加样针组件172分别设置于龙门架的两侧，当加样移动组件驱动龙门架沿加样导向组件的延伸方向运动时，龙门架带动试剂针组件171和加样针组件172沿提篮11、耗材供给模块和试剂盒载入模块14运动。
加样导向组件可设置为导轨滑块机构、导套导柱机构或齿轮齿条等常见的直线位移机构，加样移动组件则可选择驱动电机、液压缸、电动推杆等驱动机构，加样导向组件和加样移动组件的结构、尺寸、位置等根据实际检测需要参考现有技术确定，在此不再赘述。
在上述实施例的基础上，试剂针组件171包括试剂针、扎取控制组件1713、移液控制组件、试剂针升降组件以及试剂针平移组件1714，试剂针包括适配器1711和与适配器1711连通的试剂泵1712，试剂泵1712与移液控制组件连接，适配器1711与扎取控制组件1713连接，以便扎取控制组件1713控制适配器1711扎取或退出tip01/PCR管021；试剂针平移组件1714用于驱动试剂针沿水平面内龙门架组件173运动方向的垂直方向运动，试剂针升降组件用于驱动试剂针沿竖直方向运动。
请参考图18和图19，试剂针组件171内设有试剂针安装座，试剂针安装座用于安装试剂针、扎取控制组件1713、移液控制组件以及试剂针升降组件，试剂针安装座通过试剂针平移组件1714滑动连接于龙门架组件173上。
适配器1711用于与tip01或密封塞022配合连接；试剂泵1712为用于与适配器1711连接并连通的吸液泵，并与移动控制组件连接。适配器1711的种类和尺寸以及试剂泵1712的种类、尺寸、位置等根据实际检测需要参考现有技术确定，在此不再赘述。
适配器1711外套设有用于保护适配器1711的套筒1711-1，适配器1711 和套筒1711-1分别安装于适配器安装板1711-2和套筒安装板1711-3上，适配器安装板1711-2和套筒安装板1711-3之间设有弹性件，且套筒安装板1711-3与扎取控制组件1713连接，使得套筒1711-1可相对适配器1711上下滑动，从而实现对适配器1711工作状态的调节。
适配器1711与tip01连接时，套筒1711-1在tip01的端面的作用下上移，使得套筒安装板1711-3向上移动，进而驱动扎取控制组件1713上移；反之，退出tip01时，控制扎取控制组件1713带动套筒安装板1711-3下降，使得套筒1711-1沿适配器1711下降，使得tip01与适配器1711逐渐分离，直至套筒1711-1的下端面与适配器1711的下端面齐平时，tip01与适配器1711完全分离。
扎取控制组件1713由直线位移机构和驱动机构组成，直线位移机构具体可以包括导轨滑块机构、导柱导套结构等，驱动机构具体可以包括丝杆电机、步进电机、液压缸、电动推杆等电气驱动机构。
优选的，请参考图19，扎取控制组件1713包括一端与套筒安装板1711-3连接的导柱1713-1、与导柱1713-1的另一端连接的导柱连接板1713-2和丝杆电机1713-3，导柱连接板1713-2设置于丝杆电机1713-3的电机轴下方。导柱1713-1的种类、数量、尺寸和位置以及丝杆电机1713-3的种类、型号和位置等根据实际需求参考现有技术确定，在此不再赘述。
试剂针平移组件1714和试剂针升降组件包括导轨滑块机构、同步带机构等直线位移机构以及步进电机等驱动机构。请参考图18，试剂针平移组件1714包括同步带机构和驱动电机，驱动电机转动时驱动同步带以及连接于同步带上的试剂针沿Y轴方向运动。
试剂针组件171对提取试剂进行移液时，首先试剂针平移组件1714带动试剂针沿Y轴方向移动，使其正对待扎取的tip01的位置；而后，试剂针升降组件带动试剂针沿Y轴下降，使适配器1711的下端面与tip01接触，套筒1711-1在tip01的作用下上移、露出适配器1711，使得适配器1711与tip01配合连接，完成对tip01的扎取；扎取完成后，试剂针升降组件带动试剂针沿Z轴上移，试剂针平移组件1714带动试剂针移动至待吸取的试剂正上方，试剂针升降组件带动试剂针沿Z轴下移，移液控制组件控制 试剂泵1712通过连接于适配器1711上的tip01吸液；吸液完成后，试剂针升降组件带动试剂针沿Z轴上移，试剂针平移组件1714带动试剂针移动至提取试剂条03上方，由龙门架组件173带动试剂针沿X轴方向移动至反应仓031上方，试剂针升降组件带动试剂针沿Z轴下降，移液控制组件控制试剂泵1712通过适配器1711向反应仓031内注入提取试剂；注液完成后，试剂针升降组件带动试剂针沿Z轴上移，龙门架组件173带动试剂针移动至废弃孔位，扎取控制组件1713驱动套筒1711-1沿适配器1711的长度方向下移以使tip01与适配器1711分离，废弃tip01。
同理，可通过类似的操作驱动试剂针组件171向PCR管021内加注扩增试剂和提纯物。此外，试剂针组件171还可以扎取密封塞022，并利用密封塞022盖合PCR管021。
在本实施例中，试剂针升降组件和试剂针平移组件1714配合龙门架组件173实现了对适配器1711的空间坐标的调整，使得试剂针组件171可适用于提取试剂的移液、扩增试剂的移液以及PCR管021的密封；扎取控制组件1713实现了对tip01的自动扎取和废弃，提高了移液效率。
优选的，为了方便控制系统获取试剂针的工作状态，试剂针组件171还包括取tip检测组件1715和退tip检测组件1716，当导柱连接板1713-2上移至最高点处时，取tip检测组件1715输出抓取成功信号；当套筒1711-1的下端面降至与适配器1711的下端面齐平时，退tip检测组件1716输出退回成功信号。取tip检测组件1715和退tip检测组件1716的结构、尺寸、位置和连接方式等根据实际检测需要确定，在此不再赘述。
在上述实施例的基础上，加样针组件172包括若干个并列设置的移液泵1721、用于带动移液泵1721升降的移液泵升降组件1723以及用于驱动移液泵1721的柱塞相对移液泵1721的泵体滑动的柱塞升降组件1722，移液泵1721安装于移液泵升降组件1723，柱塞升降组件1722与移液泵1721的柱塞连接。
请参考图20，加样针组件172通过加样针安装座连接于龙门架组件173上，移液泵1721安装于移液泵安装板上，柱塞升降组件1722安装于抽注液固定板；移液泵安装板与抽注液固定板之间设有柱塞升降组件1722，抽 注液固定板与加样针安装座之间设有移液泵升降组件1723。
移液泵1721包括泵体和设置于泵体内的柱塞，泵体的下端用于与tip01配合；柱塞与泵体滑动连接，以便通过改变泵体的容积改变泵体内的气压、实现抽液和注液过程。移液泵1721的数量、种类、型号等请根据实际需要参考现有技术确定，在此不再赘述。
移液泵升降组件1723和柱塞升降组件1722包括直线位移机构和驱动机构，其中直线位移机构包括导轨滑块机构、同步带机构等，驱动机构包括步进电机、丝杆电机、液压缸、电动推杆等。
优选的，为了方便控制多个移液泵1721的柱塞同步运动，柱塞升降组件1722设有与移液泵1721的柱塞卡接的同步条。
加样针组件172移液时，首先龙门架组件173带动加样针组件172沿X轴运动至一次性tip耗材盒12上方，加样针升降组件1723带动移液泵1721沿Z轴下降，使得tip01安装于移液泵1721的下端；移液泵1721与tip01完成配合后，加样针升降组件1723带动移液泵1721沿Z轴上升，龙门架组件173带动加样针组件172沿X轴移动至待吸取试剂上方，加样针组件1723带动移液泵1721沿Z轴下降，柱塞升降组件172带动柱塞相对泵体沿Z轴上升，完成抽液动作；抽液完成后，加样针升降组件1723带动移液泵1721沿Z轴上升，龙门架组件173带动加样针组件172沿X轴移动至待加液位置，加样针升降组件1723带动移液泵1721沿Z轴下降，柱塞升降组件172带动柱塞相对泵体沿Z轴下降，完成注液动作；注液完成后，加样针升降组件1723带动移液泵1721沿Z轴上升，龙门架组件173带动加样针组件172沿X轴移动至废弃孔位，加样针升降组件1723带动移液泵1721沿Z轴下降，柱塞升降组件172带动柱塞相对泵体沿Z轴下降，使柱塞底部的顶针露出并下压tip01，以便tip01与移液泵1721分离，完成tip01的自动废弃。
在本实施例中，加样针组件172内并列设置有若干个移液泵1721，且同步条可驱动多个移液泵1721的柱塞同步运动，实现了多通道的同步tip安装、移液和tip废弃，避免了对同种样本设置多种相同试剂测试时的大量重复性操作，节省运行时间，极大地加快了检测速度；加样针组件172结 构紧凑，节省安装空间，有利于DNA、RNA共提取及扩增系统的小型化。
优选的，加样针组件172还包括用于检测移液泵1721工作状态的抽注液检测组件1724以及用于检测移液泵升降组件1723是否复位的Z轴原点检测组件1725。抽注液检测组件1724和Z轴原点检测组件1725的种类、安装方式、位置等根据实际检测需要确定，在此不再赘述。
在上述实施例的基础上，PCR转移装置21包括用于抓取或松开PCR管021的抓手组件211、用于打开或关闭PCR扩增装置31的扩增盖314的开盖组件212、用于驱动抓手组件211与开盖组件212沿X轴方向移动的X轴组件213、用于驱动抓手组件211与开盖组件212沿Y轴方向移动的Y轴组件214以及用于驱动抓手组件211与开盖组件212沿Z轴方向移动的Z轴组件215；抓手组件211和开盖组件212分别滑动连接于Z轴组件215，Z轴组件215滑动连接于Y轴组件214，Y轴组件214滑动连接于X轴组件213。
需要进行说明的是，抓手组件211和开盖组件212分别与Z轴组件215滑动连接，也即二者之间无直接连接关系。
X轴组件213、Y轴组件214和Z轴组件215均包括直线位移机构和驱动机构，直线位移机构为同步带机构、导轨滑块机构等直线位移机构中的一种或几种的组合，驱动机构包括步进电机、电动推杆、液压缸等常见的驱动机构。X轴组件213、Y轴组件214和Z轴组件215三者的具体结构、尺寸、位置、连接方式等根据实际检测需要确定，在此不再赘述。
PCR转移装置21工作时，X轴组件213和Y轴组件214驱动Z轴组件215运动至PCR扩增装置31的扩增加热块311正上方；Z轴组件215驱动开盖组件212沿Z轴向下运动，使开盖组件212与扩增盖314卡接固定；Y轴组件214驱动抓手组件211和开盖组件212沿Y轴方向移动，使扩增盖314与PCR扩增装置31的扩增加热块311分离，完成开盖操作；
Z轴组件215驱动抓手组件211下降至低位(抓取位)、开盖组件212上升至高位；X轴组件213和Y轴组件214驱动抓手组件211移动至PCR耗材盒13位置，抓手组件211抓取PCR管021；X轴组件213和Y轴组件214驱动抓手组件211移动至PCR扩增装置31的扩增加热块311，抓手 组件211松开PCR管021，使PCR管021被放入扩增加热块311的扩增孔内；
待反应仓031内的PCR管021放满后，Z轴组件215驱动开盖组件212下降至低位、抓手组件211上升至高位，开盖组件212闭合扩增盖314。
扩增反应完成后，PCR转移装置21按照上述相似的操作，将反应后的PCR管021取出，并丢弃至废弃位，最后再关闭扩增盖314。
在本实施例中，PCR转移装置21实现了PCR管021的三维空间移动以及扩增盖314开闭的自动化操作，且抓手组件211和开盖组件212采用同一电机控制、交替往复上下运动，有利于减小装置体积、提高抓取效率，有效地提高了PCR转移装置21的操作效率和使用效果。
在上述实施例的基础上，抓手组件211包括抓手底板、平行设于抓手底板下方的抓手活动板、用于抓取PCR管021的抓手以及用于控制抓手张开或收缩的收缩驱动组件，收缩驱动组件设于抓手底板上，收缩驱动组件的伸缩端穿过抓手底板与抓手活动板连接、以驱动抓手活动板上升或下降；抓手底板和抓手活动板均与收缩驱动组件连接，当抓手活动板下降时抓手张开，当抓手活动板上升时抓手收缩。
抓手底板、抓手活动板、抓手以及收缩驱动组件的形状、结构、尺寸、材质、位置等根据实际情况和实际需求确定。
当收缩驱动组件进行伸缩移动时，收缩驱动组件的伸缩端驱动抓手活动板下降、带动抓手张开，方便抓手抓取PCR管021；当收缩驱动组件的伸缩端驱动抓手活动板上升时，抓手活动板带动抓手收缩闭合，以便抓手抓紧PCR管021。抓手抓紧PCR管021后，X轴组件213、Y轴组件214和Z轴组件215带动PCR管021移动至所需位置。
在本实施例中，PCR管转移装置21有效地提高了PCR管021的抓取转移效率，并保证了抓取PCR管021的抓取转移操作的准确性，且结构简单、操作方便、便于推广。
为了避免移动过程中PCR管021中的核酸样品被污染，利用密封塞022密封PCR管021，并在PCR管021转移到位后打开密封塞022。
优选的，抓手组件211还包括用于和PCR管021顶部的密封塞022卡 接的导向柱以及顶套，顶套用于顶退密封塞022；导向柱穿过抓手活动板后与抓手底板固定连接，且导向柱位于抓手的轴线上；顶套套设于导向柱的外周部且顶套与抓手活动板固定连接。
当收缩驱动组件驱动抓手活动板下降时，抓手张开且固定于抓手活动板上的顶套随之下降，顶套将PCR管021顶部的密封塞022顶退，实现PCR管021的顶退；当收缩驱动组件驱动抓手活动板上升时，抓手收缩闭合且导向柱固定不动，顶套随抓手活动板向上运动，此时的导向柱露出并插入密封塞022内，使得导向柱和密封塞022卡接固定，实现抓取PCR管021的抓取。抓手抓紧PCR管021后，X轴组件213、Y轴组件214和Z轴组件215带动PCR管021移动至所需位置。
通过收缩驱动组件驱动抓手和顶套运动，以便抓手和导向柱配合抓取PCR管021、顶套有效顶退PCR管021，有效地提高了装置的操作效率，防止了人工干预造成的污染。此外，有助于减小装置体积，占用空间更小。
优选的，抓手组件211还包括用于检测密封塞022是否被成功顶退的顶退检测组件。顶推检测组件的结构、尺寸、位置等根据实际需要进行确定，在此不再赘述。
在上述实施例的基础上，PCR扩增装置31包括扩增加热块311、扩增加热片312、扩增散热器313、用于盖合扩增加热块311的扩增盖314以及用于提高扩增散热器313的散热效率的扩增冷却组件315，扩增加热块311设有至少两个用于放置PCR管021的扩增孔，扩增盖314、扩增加热块311、扩增加热片312以及扩增散热器313由上至下依次设置。
请参考图25，扩增加热块311的数量可以为一个、两个或多个，每个扩增加热块311内可设有多个扩增孔；每个扩增加热块311对应一个扩增加热片312进行加热，以降低装置成本；扩增散热器313下方设有扩增冷却组件315，以便提高扩增散热器313的散热效率，从而提高PCR扩增温度升降速度，减少扩增时间。
优选的，扩增加热块311设有用于实时检测扩增加热块311的温度的扩增温度传感器，以便于检测人员实时掌握扩增加热块311的温度，从而对加热温度进行有效控制。
扩增加热块311、扩增加热片312、扩增散热器313、扩增盖314、扩增冷却组件315以及扩增温度传感器的形状、结构、尺寸、材质、位置等根据实际需要参考现有技术确定。
PCR扩增装置31工作时，首先打开扩增盖314，将装有核酸样品的PCR管021放入扩增加热块311的扩增孔内；待PCR管021的放置完成后，盖上扩增盖314，并利用扩增加热片312对扩增加热块311进行加热，以便于对核酸样品进行PCR扩增操作。
在对同一核酸样品进行多项检测时，需要将单份核酸分为多份进行扩增，而本实施例提供的PCR扩增装置31在单个扩增加热块311设有多个扩增孔且每个扩增加热块311内加热温度保持一致，有利于保证相同核酸样品的扩增情况相同，降低了扩增温度的不同带来的样品的扩增结果偏差；扩增盖314盖合扩增加热块311后，可在扩增过程中对扩增加热块311和样品进行保温，使得温升速度更快、扩增效率更高；此外，扩增冷却组件315有效地提高了扩增散热器313的散热效率，进而提高了样品的扩增温度升降速度，减少样品的扩增时间，提高样品的扩增效率。
优选的，扩增盖314包括框架和可沿框架横向滑动的顶盖，顶盖和扩增加热块311一一对应设置，框架设有用于检测顶盖是否打开的开盖传感器，顶盖设有用于触发开盖传感器的开盖挡片。框架、顶盖、开盖传感器和开盖挡片的结构、材质、尺寸和位置等根据实际需要确定，在此不再赘述。
关盖状况下，开盖挡片和开盖传感器的位置对应，开盖挡片触发开盖传感器；开盖状态下，开盖挡片随着顶盖滑动，开盖挡片和开盖传感器分离，开盖挡片不会触发开盖传感器。
实时荧光定量检测PCR技术指在PCR反应体系中加入荧光基团，利用荧光信号积累实时监测整个PCR进程，最后通过标准曲线对未知模板进行定量分析的方法。该技术借助荧光信号检测PCR产物，一方面提高了聚合酶链式反应灵敏度，另一方面实现了每循环一次聚合酶链式反应收集一个数据，故能够建立实时核酸样品扩增曲线、准确地确定CT值，从而根据CT值确定起始的核酸拷贝数，真正实现了核酸定量检测。
在上述实施例的基础上，荧光检测装置32包括用于提供荧光光源的光源组件321、用于进行定量检测的荧光检测组件322、光源光纤323以及荧光检测光纤324，扩增孔的一侧与光源光纤323的一端连接，扩增孔的另一侧与荧光检测光纤324的一端连接；光源光纤323的另一端与光源组件321连接，荧光检测光纤324的另一端与荧光检测组件322连接。
光源组件321、荧光检测组件322、光源光纤323以及荧光检测光纤324的结构、材质、尺寸、位置和连接方式等根据实际检测需要确定。
优选的，光源光纤323和荧光检测光纤324分别连接于扩增加热块311的两侧，且光源光纤323和荧光检测光纤324的夹角为90°，以使各光纤准确牢靠定位，有利于测光值的准确性。
需要进行说明的是，本申请文件中提到第一磁铁和第二磁铁以及第一磁吸面1621-1和第二磁吸面1621-2中的第一和第二仅用于区分位置的不同，而不含对顺序的限定。
本说明书中各个实施例采用递进的方式描述，每个实施例重点说明的都是与其他实施例的不同之处，各个实施例之间相同相似部分互相参见即可。
以上对本发明所提供的DNA、RNA核酸共提取及检测系统进行了详细介绍。本文中应用了具体个例对本发明的原理及实施方式进行了阐述，以上实施例的说明只是用于帮助理解本发明的方法及其核心思想。应当指出，对于本技术领域的普通技术人员来说，在不脱离本发明原理的前提下，还可以对本发明进行若干改进和修饰，这些改进和修饰也落入本发明权利要求的保护范围内。
Claims (13)
- 一种DNA、RNA核酸共提取及检测系统，其特征在于，包括核酸提取系统、封口转运混匀系统、核酸检测系统和控制系统，所述核酸提取系统、所述封口转运混匀系统和所述核酸检测系统均与所述控制系统连接；所述核酸提取系统包括用于装载提取试剂条(03)和样本管(04)的提篮(11)、耗材供给模块、试剂盒载入模块(14)、用于自动录入条码信息的条码扫描装置(15)、磁吸温育装置(16)以及用于向所述提取试剂条(03)的反应仓(031)或PCR管(021)内移液并密封所述PCR管(021)的自动加样装置(17)；所述封口转运系统包括用于抓取并带动所述PCR管(021)移动的PCR转移装置(21)以及高速混匀装置(22)；所述核酸检测系统包括PCR扩增装置(31)和荧光检测装置(32)。
- 根据权利要求1所述的DNA、RNA核酸共提取及检测系统，其特征在于，所述提篮(11)包括提篮盖板(112)、用于盛放提取试剂条(03)的提篮底板(111)以及用于盛放样本管(04)的样本座(113)，所述样本座(113)与所述提篮底板(111)可拆卸连接；所述提篮盖板(112)与所述提篮底板(111)可转动连接，以便在所述提篮盖板(112)与所述提篮底板(111)贴合时限制所述提取试剂条(03)在高度方向上的位移。
- 根据权利要求1所述的DNA、RNA核酸共提取及检测系统，其特征在于，所述条码扫描装置(15)包括安装支架(151)、扫描组件(153)、用于在扫码位和待机位之间推动提取试剂条(03)的推手组件(154)、带动所述推手组件(154)沿所述安装支架(151)的长度方向运动的推手移动组件(152)以及检测组件(155)，所述检测组件(155)用于判断当前通道是否存在提取试剂条(03)和样本管(04)，所述检测组件(155)与所述推手移动组件(152)信号连接，所述扫描组件(153)、所述检测组件(155)均安装于所述推手组件(154)的安装底板上。
- 根据权利要求1所述的DNA、RNA核酸共提取及检测系统，其特征在于，所述磁吸温育装置(16)包括用于加热反应仓(031)的温育模块 (161)、用于对所述反应仓(031)进行磁吸的磁吸模块(162)以及驱动组件(163)，所述温育模块(161)、所述磁吸模块(162)均与所述驱动组件(163)连接，所述驱动组件(163)用于驱动所述温育模块(161)和所述磁吸模块(162)交替靠近所述反应仓(031)。
- 根据权利要求4所述的DNA、RNA核酸共提取及检测系统，其特征在于，所述温育模块(161)包括设有试剂槽(1611-1)的加热块(1611)、贴合所述试剂槽(1611-1)的内壁面设置的加热组件(1612)、设置于所述加热块(1611)底部的散热组件(1613)以及用于检测所述加热块(1611)温度的温度传感器(1614)，所述温度传感器(1614)与所述加热块(1611)连接。
- 根据权利要求4所述的DNA、RNA核酸共提取及检测系统，其特征在于，所述磁吸模块(162)包括磁铁(1621)以及用于驱动所述磁铁(1621)转动的旋转组件(1622)，所述旋转组件(1622)与所述磁铁(1621)连接；所述磁铁(1621)包括第一磁吸面(1621-1)和第二磁吸面(1621-2)，所述第一磁吸面(1621-1)和所述第二磁吸面(1621-2)二者面积大小不同且均用于吸附反应仓(031)内的磁珠。
- 根据权利要求1所述的DNA、RNA核酸共提取及检测系统，其特征在于，所述PCR转移装置(21)包括用于抓取或松开PCR管(021)的抓手组件(211)、用于打开或关闭所述PCR扩增装置(31)的扩增盖(314)的开盖组件(212)、用于驱动所述抓手组件(211)与所述开盖组件(212)沿X轴方向移动的X轴组件(213)、用于驱动所述抓手组件(211)与所述开盖组件(212)沿Y轴方向移动的Y轴组件(214)以及用于驱动所述抓手组件(211)与所述开盖组件(212)沿Z轴方向移动的Z轴组件(215)；所述抓手组件(211)和所述开盖组件(212)分别滑动连接于所述Z轴组件(215)，所述Z轴组件(215)滑动连接于所述Y轴组件(214)，所述Y轴组件(214)滑动连接于所述X轴组件(213)。
- 根据权利要求7所述的DNA、RNA核酸共提取及检测系统，其特征在于，所述抓手组件(211)包括抓手底板、平行设于所述抓手底板下方的抓手活动板、用于抓取PCR管(021)的抓手以及用于控制所述抓手张 开或收缩的收缩驱动组件，所述收缩驱动组件设于所述抓手底板上，所述收缩驱动组件的伸缩端穿过所述抓手底板与所述抓手活动板连接、以驱动所述抓手活动板上升或下降；所述抓手底板和所述抓手活动板均与所述收缩驱动组件连接，当所述抓手活动板下降时所述抓手张开，当所述抓手活动板上升时所述抓手收缩。
- 根据权利要求1所述的DNA、RNA核酸共提取及检测系统，其特征在于，所述PCR扩增装置(31)包括扩增加热块(311)、扩增加热片(312)、扩增散热器(313)、用于盖合所述扩增加热块(311)的扩增盖(314)以及用于提高所述扩增散热器(313)的散热效率的扩增冷却组件(315)，所述扩增加热块(311)设有至少两个用于放置PCR管(021)的扩增孔，所述扩增盖(314)、所述扩增加热块(311)、所述扩增加热片(312)以及所述扩增散热器(313)由上至下依次设置。
- 根据权利要求9所述的DNA、RNA核酸共提取及检测系统，其特征在于，所述荧光检测装置(32)包括用于提供荧光光源的光源组件(321)、用于进行定量检测的荧光检测组件(322)、光源光纤(323)以及荧光检测光纤(324)，所述扩增孔的一侧与所述光源光纤(323)的一端连接，所述扩增孔的另一侧与所述荧光检测光纤(324)的一端连接；所述光源光纤(323)的另一端与所述光源组件(321)连接，所述荧光检测光纤(324)的另一端与所述荧光检测组件(322)连接。
- 根据权利要求1-10任一项所述的DNA、RNA核酸共提取及检测系统，其特征在于，所述自动加样装置(17)包括用于试剂的抽注液操作的试剂针组件(171)、用于样本液的抽注液操作的加样针组件(172)以及龙门架组件(173)，所述试剂针组件(171)、所述加样针组件(172)、所述龙门架组件(173)均与所述控制系统连接；所述试剂针组件(171)和所述加样针组件(172)分别设置于所述龙门架组件(173)的两侧，所述龙门架组件(173)可沿所述提篮(11)、所述耗材供给模块以及所述试剂盒载入模块(14)运动。
- 根据权利要求11所述的DNA、RNA核酸共提取及检测系统，其特征在于，所述试剂针组件(171)包括试剂针、扎取控制组件(1713)、 移液控制组件、试剂针升降组件以及试剂针平移组件(1714)，所述试剂针包括适配器(1711)以及与所述适配器(1711)连通的试剂泵(1712)，所述试剂泵(1712)与移液控制组件连接，所述适配器(1711)与所述扎取控制组件(1713)连接，以便所述扎取控制组件(1713)控制所述适配器(1711)扎取或退出tip(01)或密封塞(022)；所述试剂针平移组件(1714)用于驱动所述试剂针沿水平面内、所述龙门架组件(173)运动方向的垂直方向运动，所述试剂针升降组件用于驱动所述试剂针沿竖直方向运动。
- 根据权利要求11所述的DNA、RNA核酸共提取及检测系统，其特征在于，所述加样针组件(172)包括若干个并列设置的移液泵(1721)、用于带动所述移液泵(1721)升降的移液泵升降组件(1723)以及用于驱动所述移液泵(1721)的柱塞相对所述移液泵(1721)的泵体滑动的柱塞升降组件(1722)，所述移液泵(1721)安装于所述移液泵升降组件(1723)，所述柱塞升降组件(1722)与所述移液泵(1721)的柱塞连接。
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US18/560,380 US20240240171A1 (en) | 2021-05-13 | 2021-12-24 | Dna and rna nucleic acid co-extraction and detection system |
| EP21941743.3A EP4339278A1 (en) | 2021-05-13 | 2021-12-24 | Dna and rna nucleic acid co-extraction and detection system |
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| CN117551541A (zh) * | 2023-11-22 | 2024-02-13 | 鹍远泰州医学检验实验室有限公司 | 一种基于分子检测用的荧光定量pcr仪 |
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| CN113150977B (zh) * | 2021-05-13 | 2024-05-07 | 安图实验仪器(郑州)有限公司 | 一种dna、rna核酸共提取及检测系统 |
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103789198A (zh) * | 2014-02-27 | 2014-05-14 | 苏州天隆生物科技有限公司 | 全自动核酸提取仪 |
| WO2015072941A1 (en) * | 2013-11-15 | 2015-05-21 | Anatolia Tani Ve Biyoteknoloji Urunleri Arastirma Gelistirme Sanayi Ve Ticaret Anonim Sirketi | Laboratory automation system |
| CN106520540A (zh) * | 2016-12-08 | 2017-03-22 | 湖南圣湘生物科技有限公司 | 一体化全自动核酸检测反应系统 |
| CN109337793A (zh) * | 2018-10-23 | 2019-02-15 | 宁波艾捷康宁生物科技有限公司 | 一种全自动核酸提取检测系统 |
| CN212504899U (zh) * | 2020-05-15 | 2021-02-09 | 北京百康芯生物科技有限公司 | 一种全自动核酸检测分析系统 |
| CN112694970A (zh) * | 2020-12-29 | 2021-04-23 | 安图实验仪器(郑州)有限公司 | 一种核酸提取及荧光pcr检测系统 |
| CN113150977A (zh) * | 2021-05-13 | 2021-07-23 | 安图实验仪器(郑州)有限公司 | 一种dna、rna核酸共提取及检测系统 |
| CN214991615U (zh) * | 2021-05-13 | 2021-12-03 | 安图实验仪器(郑州)有限公司 | 一种dna、rna核酸共提取及检测系统 |
-
2021
- 2021-05-13 CN CN202110523722.5A patent/CN113150977B/zh active Active
- 2021-12-24 US US18/560,380 patent/US20240240171A1/en active Pending
- 2021-12-24 EP EP21941743.3A patent/EP4339278A1/en active Pending
- 2021-12-24 WO PCT/CN2021/141190 patent/WO2022237181A1/zh active Application Filing
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015072941A1 (en) * | 2013-11-15 | 2015-05-21 | Anatolia Tani Ve Biyoteknoloji Urunleri Arastirma Gelistirme Sanayi Ve Ticaret Anonim Sirketi | Laboratory automation system |
| CN103789198A (zh) * | 2014-02-27 | 2014-05-14 | 苏州天隆生物科技有限公司 | 全自动核酸提取仪 |
| CN106520540A (zh) * | 2016-12-08 | 2017-03-22 | 湖南圣湘生物科技有限公司 | 一体化全自动核酸检测反应系统 |
| CN109337793A (zh) * | 2018-10-23 | 2019-02-15 | 宁波艾捷康宁生物科技有限公司 | 一种全自动核酸提取检测系统 |
| CN212504899U (zh) * | 2020-05-15 | 2021-02-09 | 北京百康芯生物科技有限公司 | 一种全自动核酸检测分析系统 |
| CN112694970A (zh) * | 2020-12-29 | 2021-04-23 | 安图实验仪器(郑州)有限公司 | 一种核酸提取及荧光pcr检测系统 |
| CN113150977A (zh) * | 2021-05-13 | 2021-07-23 | 安图实验仪器(郑州)有限公司 | 一种dna、rna核酸共提取及检测系统 |
| CN214991615U (zh) * | 2021-05-13 | 2021-12-03 | 安图实验仪器(郑州)有限公司 | 一种dna、rna核酸共提取及检测系统 |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111197004A (zh) * | 2020-01-20 | 2020-05-26 | 苏州汇瑞康医疗科技有限公司 | 一种全自动实时荧光定量pcr工作站 |
| CN115992039A (zh) * | 2023-03-21 | 2023-04-21 | 深圳市美德瑞生物科技有限公司 | 一种下部磁吸自动核酸提取装置 |
| CN115992039B (zh) * | 2023-03-21 | 2023-06-16 | 深圳市美德瑞生物科技有限公司 | 一种下部磁吸自动核酸提取装置 |
| CN116106500A (zh) * | 2023-04-12 | 2023-05-12 | 河北沧美园林绿化工程有限公司 | 一种农林水系检测的cod检测设备 |
| CN116478808A (zh) * | 2023-06-21 | 2023-07-25 | 长沙迈迪克智能科技有限公司 | 移液机器人、控制方法及存储介质 |
| CN116478808B (zh) * | 2023-06-21 | 2023-09-19 | 长沙迈迪克智能科技有限公司 | 移液机器人、控制方法及存储介质 |
| CN117405912A (zh) * | 2023-10-25 | 2024-01-16 | 上海品峰医疗科技有限公司 | 一种样本自动分析装置及其分析方法、试剂盒更换方法 |
| CN117551541A (zh) * | 2023-11-22 | 2024-02-13 | 鹍远泰州医学检验实验室有限公司 | 一种基于分子检测用的荧光定量pcr仪 |
| CN117551541B (zh) * | 2023-11-22 | 2024-06-07 | 鹍远泰州医学检验实验室有限公司 | 一种基于分子检测用的荧光定量pcr仪 |
Also Published As
| Publication number | Publication date |
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| EP4339278A1 (en) | 2024-03-20 |
| US20240240171A1 (en) | 2024-07-18 |
| CN113150977A (zh) | 2021-07-23 |
| CN113150977B (zh) | 2024-05-07 |
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