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WO2022207014A3 - 抗真菌(1，3)-β-D葡聚糖单克隆抗体、其编码基因及其表达和应用 - Google Patents

抗真菌(1，3)-β-D葡聚糖单克隆抗体、其编码基因及其表达和应用 Download PDF

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Publication number
WO2022207014A3
WO2022207014A3 PCT/CN2022/107379 CN2022107379W WO2022207014A3 WO 2022207014 A3 WO2022207014 A3 WO 2022207014A3 CN 2022107379 W CN2022107379 W CN 2022107379W WO 2022207014 A3 WO2022207014 A3 WO 2022207014A3
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WIPO (PCT)
Prior art keywords
seq
antifungal
expression
monoclonal antibody
coding gene
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PCT/CN2022/107379
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English (en)
French (fr)
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WO2022207014A2 (zh
Inventor
檀绍晨
李宁
彭波
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河北康立德生物科技有限公司
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Application filed by 河北康立德生物科技有限公司 filed Critical 河北康立德生物科技有限公司
Publication of WO2022207014A2 publication Critical patent/WO2022207014A2/zh
Priority to US18/070,516 priority Critical patent/US20230340088A1/en
Publication of WO2022207014A3 publication Critical patent/WO2022207014A3/zh

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/14—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from fungi, algea or lichens
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56961—Plant cells or fungi
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/583—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with non-fluorescent dye label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/37—Assays involving biological materials from specific organisms or of a specific nature from fungi
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
    • G01N2400/10—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • G01N2400/12—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar
    • G01N2400/24—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar beta-D-Glucans, i.e. having beta 1,n (n=3,4,6) linkages between saccharide units, e.g. xanthan
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2469/00—Immunoassays for the detection of microorganisms
    • G01N2469/10—Detection of antigens from microorganism in sample from host
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

本申请提供一种抗真菌(1，3)-β-D葡聚糖单克隆抗体、其编码基因及其表达和应用，属于生物和医疗检测技术领域，所述抗体包括轻链可变区决定簇互补区，其氨基酸序列包括：SEQ ID NO：1所示的VL-CDR1、SEQ ID NO：2所示的VL-CDR2和SEQ ID NO：3所示的VL-CDR3；所述抗体还包括重链可变区决定簇互补区，其氨基酸序列包括：SEQ ID NO：4所示的VH-CDR1、SEQ ID NO：5所示的VH-CDR2和SEQ ID NO：6所示的VH-CDR3。该抗体与真菌(1，3)-β-D葡聚糖特异性结合，抗体亲和力高，不与干扰物质产生交叉反应。
PCT/CN2022/107379 2021-09-07 2022-07-22 抗真菌(1，3)-β-D葡聚糖单克隆抗体、其编码基因及其表达和应用 WO2022207014A2 (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US18/070,516 US20230340088A1 (en) 2021-09-07 2022-11-29 Fungal (1,3)-Beta-D glucan-directed monoclonal antibody, coding genes thereof, expression thereof and application thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202111045511.1A CN113929776B (zh) 2021-09-07 2021-09-07 抗真菌(1，3)-β-D葡聚糖单克隆抗体、其编码基因及其表达和应用
CN202111045511.1 2021-09-07

Related Child Applications (1)

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US18/070,516 Continuation-In-Part US20230340088A1 (en) 2021-09-07 2022-11-29 Fungal (1,3)-Beta-D glucan-directed monoclonal antibody, coding genes thereof, expression thereof and application thereof

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WO2022207014A2 WO2022207014A2 (zh) 2022-10-06
WO2022207014A3 true WO2022207014A3 (zh) 2022-12-08

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US (1) US20230340088A1 (zh)
CN (1) CN113929776B (zh)
WO (1) WO2022207014A2 (zh)
ZA (1) ZA202210155B (zh)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113929776B (zh) * 2021-09-07 2023-06-27 河北康立德生物科技有限公司 抗真菌(1，3)-β-D葡聚糖单克隆抗体、其编码基因及其表达和应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020173035A1 (en) * 2001-05-16 2002-11-21 Lee Jong-Wook Variable region of the monoclonal antibody against the HBV S-surface antigen and a gene encoding the same
CN110885372A (zh) * 2019-12-17 2020-03-17 丹娜（天津）生物科技有限公司 一种抗念珠菌甘露聚糖的兔源单克隆抗体及其应用
CN112940114A (zh) * 2021-02-09 2021-06-11 丹娜(天津)生物科技股份有限公司 一种抗真菌1,3-β-D-葡聚糖单克隆抗体及其应用
CN113929776A (zh) * 2021-09-07 2022-01-14 河北康立德生物科技有限公司 抗真菌(1，3)-β-D葡聚糖单克隆抗体、其编码基因及其表达和应用

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Publication number Priority date Publication date Assignee Title
WO1996006858A1 (fr) * 1994-09-01 1996-03-07 Seikagaku Corporation PROTEINE FIXANT LE (1→3)-β-D-GLUCANE, ANTICORPS RECONNAISSANT LA PROTEINE ET UTILISATION DE LA PROTEINE ET DE L'ANTICORPS
CN113249336B (zh) * 2021-07-15 2021-10-08 天津一瑞生物科技股份有限公司 鼠抗（1,3）-β-D葡聚糖杂交瘤细胞株，单克隆抗体及应用

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020173035A1 (en) * 2001-05-16 2002-11-21 Lee Jong-Wook Variable region of the monoclonal antibody against the HBV S-surface antigen and a gene encoding the same
CN110885372A (zh) * 2019-12-17 2020-03-17 丹娜（天津）生物科技有限公司 一种抗念珠菌甘露聚糖的兔源单克隆抗体及其应用
CN112940114A (zh) * 2021-02-09 2021-06-11 丹娜(天津)生物科技股份有限公司 一种抗真菌1,3-β-D-葡聚糖单克隆抗体及其应用
CN113929776A (zh) * 2021-09-07 2022-01-14 河北康立德生物科技有限公司 抗真菌(1，3)-β-D葡聚糖单克隆抗体、其编码基因及其表达和应用

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WO2022207014A2 (zh) 2022-10-06
CN113929776B (zh) 2023-06-27
CN113929776A (zh) 2022-01-14
US20230340088A1 (en) 2023-10-26
ZA202210155B (en) 2022-09-28

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