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WO2022012445A1 - Quantitative liquid-taking mechanism and micro-fluidic device - Google Patents

Quantitative liquid-taking mechanism and micro-fluidic device Download PDF

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Publication number
WO2022012445A1
WO2022012445A1 PCT/CN2021/105627 CN2021105627W WO2022012445A1 WO 2022012445 A1 WO2022012445 A1 WO 2022012445A1 CN 2021105627 W CN2021105627 W CN 2021105627W WO 2022012445 A1 WO2022012445 A1 WO 2022012445A1
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WO
WIPO (PCT)
Prior art keywords
liquid
passage
connecting vent
cavity channel
taking mechanism
Prior art date
Application number
PCT/CN2021/105627
Other languages
French (fr)
Inventor
Pinhong LIANG
Zhaorui ZHOU
Cheng Liu
Original Assignee
Shenzhen Hailife Biotechnology Co., Ltd.
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Filing date
Publication date
Application filed by Shenzhen Hailife Biotechnology Co., Ltd. filed Critical Shenzhen Hailife Biotechnology Co., Ltd.
Publication of WO2022012445A1 publication Critical patent/WO2022012445A1/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
    • G01N35/1016—Control of the volume dispensed or introduced
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5029—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures using swabs
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01F—MEASURING VOLUME, VOLUME FLOW, MASS FLOW OR LIQUID LEVEL; METERING BY VOLUME
    • G01F11/00—Apparatus requiring external operation adapted at each repeated and identical operation to measure and separate a predetermined volume of fluid or fluent solid material from a supply or container, without regard to weight, and to deliver it
    • G01F11/02—Apparatus requiring external operation adapted at each repeated and identical operation to measure and separate a predetermined volume of fluid or fluent solid material from a supply or container, without regard to weight, and to deliver it with measuring chambers which expand or contract during measurement
    • G01F11/021—Apparatus requiring external operation adapted at each repeated and identical operation to measure and separate a predetermined volume of fluid or fluent solid material from a supply or container, without regard to weight, and to deliver it with measuring chambers which expand or contract during measurement of the piston type
    • G01F11/029—Apparatus requiring external operation adapted at each repeated and identical operation to measure and separate a predetermined volume of fluid or fluent solid material from a supply or container, without regard to weight, and to deliver it with measuring chambers which expand or contract during measurement of the piston type provided with electric controlling means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1002—Reagent dispensers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838—Capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0457—Moving fluids with specific forces or mechanical means specific forces passive flow or gravitation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0478—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure pistons
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
    • G01N2035/1025—Fluid level sensing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1034—Transferring microquantities of liquid

Definitions

  • Embodiments of the present disclosure generally relate to the field of liquid measuring and taking, and particularly relate to a quantitative liquid-taking mechanism and a micro-fluidic device.
  • IVD in vitro diagnostic
  • One of them to add liquid is using devices such as injection pump or peristaltic pump.
  • the amount of sample is controlled by the movement of mechanical parts.
  • the movement of the mechanical parts can give a certain airspace, which will be replaced by the liquid, however, the perfect accuracy of adding liquid volume is hardly to be achieved due to the structures of these devices.
  • the other way to add liquid is using a pipettor or a dropper.
  • This method also has some defects, mainly defect is that the pipettor has a high adding accuracy, but its price is also high. in contrast the dropper is cheaper, but it is hard to ensure accuracy.
  • one object of the present disclosure is at least to provide a quantitative liquid-taking mechanism, which could improve the accuracy of liquid adding, while reducing the structural complexity and the cost of manufacturing.
  • another object of the present disclosure is to provide a micro-fluidic device having the above-mentioned quantitative liquid-taking mechanism.
  • the present disclosure is realized through following technical solutions.
  • a quantitative liquid-taking mechanism which includes:
  • a liquid carrying rod having a passage formed therein and configured for quantitatively measuring and taking, and transporting a target liquid
  • a cavity channel sleeved on the liquid carrying rod and having a first connecting vent, a second connecting vent and a third connecting vent, wherein the third connecting vent is disposed between the second connecting vent and a bottom of the cavity channel, when the liquid carrying rod moves in the cavity channel to a predetermined position, one end of the passage is connected to the first connecting vent, and the other end of the passage is connected to the second connecting vent;
  • a fluid-passage configured for receiving and outputting the target liquid flowing from the first connecting vent.
  • the passage has a rectilinear shape.
  • a flow direction of the passage is vertical to a length direction of the liquid carrying rod.
  • the first connecting vent and the second connecting vent are symmetrically disposed on two sides of the cavity channel.
  • a concave portion is formed on the bottom of the cavity channel and sunken towards interior of the cavity channel.
  • the cavity channel, the air-passage and the fluid-passage are all located in a base.
  • the quantitative liquid-taking mechanism further includes a balancing hole and a liquid-injecting hole, the balancing hole and the liquid-injecting hole are connected to the cavity channel and are disposed at an area between the first connecting vent, the second connecting vent and an entrance of the cavity channel;
  • the balancing hole and the liquid-injecting hole are symmetrically disposed on two sides of the cavity channel.
  • a micro-fluidic device which includes:
  • Embodiments of the present disclosure at least have following advantages:
  • the quantitative liquid-taking mechanism can finish the sampling work with measuring and taking liquid accurately through several simple structures, such as liquid carrying rod, cavity channel, air-passage and fluid-passage.
  • the quantitative liquid-taking mechanism is designed simply in structural, easy to be manufactured with control cost.
  • these structures, such as liquid carrying rod, cavity channel, air-passage and fluid-passage are disposed on a base, which can further simplify the structural design.
  • the mechanism further includes a balancing hole and a liquid-injecting hole, which are used for adding liquid into the passage and would further improve accuracy of liquid adding process.
  • Fig. 1 is a schematic structural view of a quantitative liquid-taking mechanism according to some embodiments of the present disclosure
  • Fig. 2 is a schematic structural view of a liquid carrying rod according to some embodiments of the present disclosure
  • Fig. 3 is a schematic structural view of a quantitative liquid-taking mechanism according to some embodiments of the present disclosure.
  • Fig. 4 is a schematic structural view of a quantitative liquid-taking mechanism according to some embodiments of the present disclosure.
  • first , “second” and “third” are used herein for purposes of description only and are not intended to indicate or imply relative importance or significance, also not to impliedly indicate the quantity of the technical features referred to.
  • the features defined with “first” , “second” and “third” may comprise one or more these features.
  • “aplurality of” means two or more of these features, unless specified.
  • terms such as “above” , “below” , “top” , “bottom” , “inner” , and “outer” should be construed to the orientation or relative position as described or as shown in the drawings.
  • a quantitative liquid-taking mechanism 10 which includes:
  • a liquid carrying rod 100 having a passage 110 formed therein and configured for quantitatively measuring and taking, and transporting a target liquid
  • a cavity channel 210 sleeved on the liquid carrying rod 100 and having a first connecting vent 211, a second connecting vent 212 and a third connecting vent 213, wherein the third connecting vent 213 is disposed between the second connecting vent 212 and bottom of the cavity channel 210, when the liquid carrying rod 100 moves in the cavity channel 210 to a predetermined position, one end of the passage 110 is connected to the first connecting vent 211, and the other end of the passage 110 is connected to the second connecting vent 212;
  • an air-passage 220 configured to connect the second connecting vent 212 to the third connecting vent 213;
  • a fluid-passage 230 configured for receiving and outputting the target liquid flowing from the first connecting vent 211.
  • the passage 110 has a rectilinear shape.
  • the passage 110 may have other different shape, such as curved shape.
  • one with ordinary skills in the art may design the size of the passage 110 according to actual needs of measuring range, that is, at least one function of the passage 110 is to accurately measuring and taking a target liquid.
  • a flow direction of the passage 110 is vertical to a length direction of the liquid carrying rod 100.
  • the passage 110 can be disposed in a non-vertical manner. Besides, two ends of the passage 110 are connected with external of the liquid carrying rod 100.
  • Shape of the cavity channel 210 and size of inner space of the cavity channel 210 are matched with the liquid carrying rod 100, after the liquid carrying rod 100 slid into the cavity channel 210, an outer wall of the liquid carrying rod 100 abuts against an inner wall of the cavity channel 210 so as to prevent the target liquid measured and taken in the liquid carrying rod 100 from flowing out through a gap which may formed between the outer wall of the liquid carrying rod 100 and the inner wall of the cavity channel 210.
  • the first connecting vent 211, the second connecting vent 212 and the third connecting vent 213 are disposed in the cavity channel 210.
  • the first connecting vent 211 and the second connecting vent 212 are symmetrically disposed on two sides of the cavity channel 210.
  • the first connecting vent 211 and the second connecting vent 212 can be unsymmetrically disposed, it can be designed by one with ordinary skills in the art according to actual needs.
  • relationship between the first connecting vent 211, the second connecting vent 212 and the third connecting vent 213 is set to be that: when the passage 110 moves to a predetermined position, that is, when the passage 110 moves to a position between the first connecting vent 211 and the second connecting vent 212, the first end of the passage 110 is connected to the first connecting vent 211, and the second end of the passage 110 is connected to the second connecting vent 212, as shown in FIG. 4, such that gases can be easily driven in via the second connecting vent 212, and the target liquid in the passage 110 can be driven into the first connecting vent 211 due to air pressure difference.
  • the third connecting vent 213 is disposed between the second connecting vent 212 and bottom of the cavity channel 210, and a first end of the air-passage 220 is connected to the second connecting vent 212, and a second end of the air-passage 220 is connected to the third connecting vent 213.
  • the liquid carrying rod 100 When the quantitative liquid-taking mechanism 10 is in actual use, the liquid carrying rod 100 is pushed to move toward the bottom of the cavity channel 210 from an entrance of the cavity channel 210, then gases in the cavity channel 210 are compressed, therefore the gases flow to the second connecting vent 212 through the third connecting vent 213, and then an air pressure difference generates between the second connecting vent 212 and the first connecting vent 211. On that basis, continue pushing the liquid carrying rod 100, until the second connecting vent 212 and the first connecting vent are connected to the passage 110 at the same time, under effect of the aforementioned air pressure difference, the target liquid in the passage 110 can be driven into the fluid-passage 230 through the first connecting vent 211.
  • the quantitative liquid-taking mechanism 10 can do sampling work with measuring and taking liquid accurately through several simple structures, such as liquid carrying rod 100, cavity channel 210, air-passage 220 and fluid-passage 230.
  • the quantitative liquid-taking mechanism s structural design is simple, and it is easy to manufacture with controlled manufacturing cost.
  • these structures, such as liquid carrying rod 100, cavity channel 210, air-passage 220 and fluid-passage 230, are disposed on a base 200, which can further simplify the structural design.
  • the cavity channel 210, the air-passage 220 and the fluid-passage 230 are all located in the base 200.
  • a concave portion 260 is formed on the bottom of the cavity channel 210 and sunken towards interior of the cavity channel 210.
  • the concave portion 260 has a concave direction toward the entrance of the cavity channel 210.
  • the function of the concave portion 260 is to maintain the connection of the cavity channel 210 and the air-passage 220 during the process of the liquid carrying rod 100 moving to bottom of the cavity channel 210 and to avoid the fluid carrying rod 100 being blocked in the cavity channel 210. Then compressed gas in the cavity channel 210 produces positive air pressure and would be continuously released through the air-passage 220, and liquid in the air-passage 220 or in the fluid-passage 230 is driven to flow into subsequent structures.
  • the quantitative liquid-taking mechanism 10 further includes a balancing hole 240 and a liquid-injecting hole 250, the balancing hole 240 and the liquid-injecting hole 250 are connected to the cavity channel 210 and are disposed at an area between the first connecting vent 211, the second connecting vent 212, and an entrance of the cavity channel 210; that is, when the liquid carrying rod 100 moves from the entrance of the cavity channel 210 toward the bottom of the cavity channel 210, it firstly passes through the balancing hole 240 and the liquid-injecting hole 250, and then passes through the first connecting vent 211 and the second connecting vent 212.
  • the main function of the balancing hole 240 and the liquid-injecting hole 250 is to add liquid into the passage 110 accurately, the specific operation can be as follows: sufficient amount of liquid is added to the liquid-injecting hole 250, and then, under effect of gravity and capillary siphon, the liquid flows from the liquid-injecting hole 250 to the balancing hole 240, and when the liquid stored on the liquid-injecting hole 250 and the balancing hole 240 reaches a state of balance, the cavity channel 110 is filled with measured liquid, so as to realize accuracy liquid-taking and liquid-adding work.
  • the balancing hole 240 and the liquid-injecting hole 250 can be disposed on the base 200.
  • the balancing hole 240 and the liquid-injecting hole 250 are symmetrically disposed on two sides of the cavity channel 210.
  • a micro-fluidic device is provided according to second aspect of the present disclosure, which includes:
  • the micro-fluidic chip can be realized depend on existing available technology.
  • micro-fluidic chip has a high requirement to the accuracy of sample liquid, on the first hand, when the quantitative liquid-taking mechanism 10 mentioned in all embodiments according to the first aspect is used together with the micro-fluidic chip, a very good practical effect can be achieved in quantitative liquid adding.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
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  • Dispersion Chemistry (AREA)
  • Fluid Mechanics (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

A quantitative liquid-taking mechanism (10), and a micro-fluidic device comprising the quantitative liquid-taking mechanism (10), the quantitative liquid-taking mechanism (10) includes: a liquid carrying rod (100) having a passage (110) formed therein and configured to quantitative measuring, taking, and transporting a target liquid; a cavity channel (210) sleeved on the liquid carrying rod (100) and having a first connecting vent (211), a second connecting vent (212) and a third connecting vent (213) formed therein; an air-passage (220) configured to connect the second connecting vent (212) to the third connecting vent (213); and a fluid-passage (230) configured for receiving and outputting the target liquid flowing from the first connecting vent (211). The third connecting vent (213) is disposed between the second connecting vent (212) and bottom of the cavity channel (210), when the liquid carrying rod (100) moves in the cavity channel (210) to a predetermined position, one end of the passage (110) is connected to the first connecting vent (211), and the other end of the passage (110) is connected to the second connecting vent (212). The quantitative liquid-taking mechanism (10) may improve accuracy of liquid-adding on the basis of reducing structural complexity and manufacturing cost.

Description

QUANTITATIVE LIQUID-TAKING MECHANISM AND MICRO-FLUIDIC DEVICE
Cross Reference to Related Application
The present application claims the priority of Chinese Application No. 202011028336.0, filed on September 26, 2020, and Chinese Application No. 202021370424.4, filed on July, 11, 2020. The entire contents of the above-identified applications are herein incorporated by reference.
TECHNICAL FIELD
Embodiments of the present disclosure generally relate to the field of liquid measuring and taking, and particularly relate to a quantitative liquid-taking mechanism and a micro-fluidic device.
BACKGROUND
In in vitro diagnostic (IVD) test, the accuracy of quantitatively adding liquid sample could directly affect the accuracy and reliability of final test results. At present, there are mainly two ways of adding fluid in clinical diagnosis.
One of them to add liquid is using devices such as injection pump or peristaltic pump. The amount of sample is controlled by the movement of mechanical parts. The movement of the mechanical parts can give a certain airspace, which will be replaced by the liquid, however, the perfect accuracy of adding liquid volume is hardly to be achieved due to the structures of these devices.
The other way to add liquid is using a pipettor or a dropper. This method also has some defects, mainly defect is that the pipettor has a high adding accuracy, but its price is also high. in contrast the dropper is cheaper, but it is hard to ensure accuracy.
SUMMARY
As stated in the above BACKGROUND, during the process of liquid adding, the  accuracy of quantitative added liquid, the complexity and the cost of liquid adding apparatus are important factors that cannot be ignored. How to design an apparatus that can balance the complexity and the cost at the same time is an important issue faced by technicians in the art.
In view of the above problems, in the present disclosure, using the piston motion and driving force produced by air pressure difference, inventors invent a quantitative liquid-taking mechanism and corresponding device to solve the above problems, which has a widely applications in in vitro diagnostic test, especially in micro-fluidic technical field.
In conclusion, one object of the present disclosure is at least to provide a quantitative liquid-taking mechanism, which could improve the accuracy of liquid adding, while reducing the structural complexity and the cost of manufacturing.
In addition, just as mentioned above, another object of the present disclosure is to provide a micro-fluidic device having the above-mentioned quantitative liquid-taking mechanism.
Specifically, the present disclosure is realized through following technical solutions.
First aspect of the present disclosure
In the present disclosure, a quantitative liquid-taking mechanism is provided, which includes:
a liquid carrying rod having a passage formed therein and configured for quantitatively measuring and taking, and transporting a target liquid;
a cavity channel sleeved on the liquid carrying rod and having a first connecting vent, a second connecting vent and a third connecting vent, wherein the third connecting vent is disposed between the second connecting vent and a bottom of the cavity channel, when the liquid carrying rod moves in the cavity channel to a predetermined position, one end of the passage is connected to the first connecting vent, and the other end of the passage is connected to the second connecting vent;
an air-passage configured to connect the second connecting vent with the third connecting vent; and
a fluid-passage configured for receiving and outputting the target liquid flowing from the first connecting vent.
In some embodiments, the passage has a rectilinear shape.
In some embodiments, a flow direction of the passage is vertical to a length direction of the liquid carrying rod.
In some embodiments, the first connecting vent and the second connecting vent are symmetrically disposed on two sides of the cavity channel.
In some embodiments, a concave portion is formed on the bottom of the cavity channel and sunken towards interior of the cavity channel.
In some embodiments, the cavity channel, the air-passage and the fluid-passage are all located in a base.
In some embodiments, the quantitative liquid-taking mechanism further includes a balancing hole and a liquid-injecting hole, the balancing hole and the liquid-injecting hole are connected to the cavity channel and are disposed at an area between the first connecting vent, the second connecting vent and an entrance of the cavity channel;
when the passage moves to another predetermined position, two ends of the passage are connected to the balancing hole and the liquid-injecting hole respectively.
In some embodiments, the balancing hole and the liquid-injecting hole are symmetrically disposed on two sides of the cavity channel.
Second aspect of the present disclosure
In the present disclosure, a micro-fluidic device is provided, which includes:
a quantitative liquid-taking mechanism according to the first aspect of the present disclosure; and
a micro-fluidic chip.
Embodiments of the present disclosure at least have following advantages:
In some embodiments, the quantitative liquid-taking mechanism can finish the sampling work with measuring and taking liquid accurately through several simple structures, such as liquid carrying rod, cavity channel, air-passage and fluid-passage. At the same time, the quantitative liquid-taking mechanism is designed simply in structural, easy to be manufactured with control cost. Besides, these structures, such as liquid carrying rod, cavity channel, air-passage and fluid-passage, are disposed on a base, which can further simplify the structural design.
In some embodiments, the mechanism further includes a balancing hole and a liquid-injecting hole, which are used for adding liquid into the passage and would  further improve accuracy of liquid adding process.
Additional advantages, objects, and characteristics of the present disclosure will be set forth in following description and will be understood by those skilled in the art through researching and practicing the present disclosure.
BRIEF DESCRIPTION OF THE DRAWINGS
Fig. 1 is a schematic structural view of a quantitative liquid-taking mechanism according to some embodiments of the present disclosure;
Fig. 2 is a schematic structural view of a liquid carrying rod according to some embodiments of the present disclosure;
Fig. 3 is a schematic structural view of a quantitative liquid-taking mechanism according to some embodiments of the present disclosure; and
Fig. 4 is a schematic structural view of a quantitative liquid-taking mechanism according to some embodiments of the present disclosure.
Reference Numerals:
10 quantitative liquid-taking mechanism; 100 liquid carrying rod; 110 passage; 200 base; 210 cavity channel; 211 first connecting vent; 212 second connecting vent; 213 third connecting vent; 220 air-passage; 230 fluid-passage; 240 balancing hole; 250 liquid-injecting hole; 260 concave portion.
DETAILED DESCRIPTION
Embodiments of the present disclosure are further described below in detail with reference to the accompanying drawings, such that one skilled in the art may implement the present disclosure according to specification of the present disclosure.
In embodiments of the present disclosure, terms such as “first” , “second” and “third” are used herein for purposes of description only and are not intended to indicate or imply relative importance or significance, also not to impliedly indicate the quantity of the technical features referred to. Thus, the features defined with “first” , “second” and “third” may comprise one or more these features. In the description of the present disclosure, “aplurality of” means two or more of these features, unless specified. In  addition, terms such as “above” , “below” , “top” , “bottom” , “inner” , and “outer” should be construed to the orientation or relative position as described or as shown in the drawings. These terms are only for convenient and concise description and comprehension, and do not indicate or imply that the device or element referred to must have a particular orientation or movement. Thus, it cannot be understood to limit the present disclosure. Moreover, terms such as “include” , “dispose” and any other deformation are used in the description intending to cover non-exclusive inclusion. For example, process, method, system, product or device including sequence of steps or units may alternative include other steps or units that are not listed, or alternative include other inherent steps or units that are inherent in these process, method, system, product or device.
Besides, it should be emphasized that, reference to “embodiment” in the present disclosure implies that specific features, structures or characters described in combination with the embodiment may be included in at least one embodiment of the present disclosure. The phrases appearing in different places in the specification may not necessarily refer to a same embodiment, nor separate or alternative embodiment that is mutually exclusive with other embodiment. It should be explicitly and implicitly understood by those with ordinary skill in the art that embodiment described herein may be combined with other embodiment.
<Quantitative liquid-taking mechanism>
As shown in Figs. 1-4, according to the first aspect of the present disclosure, a quantitative liquid-taking mechanism 10 is provided, which includes:
a liquid carrying rod 100 having a passage 110 formed therein and configured for quantitatively measuring and taking, and transporting a target liquid;
a cavity channel 210 sleeved on the liquid carrying rod 100 and having a first connecting vent 211, a second connecting vent 212 and a third connecting vent 213, wherein the third connecting vent 213 is disposed between the second connecting vent 212 and bottom of the cavity channel 210, when the liquid carrying rod 100 moves in the cavity channel 210 to a predetermined position, one end of the passage 110 is connected to the first connecting vent 211, and the other end of the passage 110 is connected to the second connecting vent 212;
an air-passage 220 configured to connect the second connecting vent 212 to the third connecting vent 213; and
a fluid-passage 230 configured for receiving and outputting the target liquid flowing from the first connecting vent 211.
Specifically, as shown in Fig. 1, the passage 110 has a rectilinear shape. In some other embodiments, the passage 110 may have other different shape, such as curved shape. Besides, one with ordinary skills in the art may design the size of the passage 110 according to actual needs of measuring range, that is, at least one function of the passage 110 is to accurately measuring and taking a target liquid. In addition, for the sake of easy designing, a flow direction of the passage 110 is vertical to a length direction of the liquid carrying rod 100. Certainly, in some other embodiments, the passage 110 can be disposed in a non-vertical manner. Besides, two ends of the passage 110 are connected with external of the liquid carrying rod 100.
Shape of the cavity channel 210 and size of inner space of the cavity channel 210 are matched with the liquid carrying rod 100, after the liquid carrying rod 100 slid into the cavity channel 210, an outer wall of the liquid carrying rod 100 abuts against an inner wall of the cavity channel 210 so as to prevent the target liquid measured and taken in the liquid carrying rod 100 from flowing out through a gap which may formed between the outer wall of the liquid carrying rod 100 and the inner wall of the cavity channel 210.
On the above basis, in order to transport the target liquid in the passage 110 of the liquid carrying rod according to a predetermined plan, the first connecting vent 211, the second connecting vent 212 and the third connecting vent 213 are disposed in the cavity channel 210.
As shown in FIG. 2, for easily design, the first connecting vent 211 and the second connecting vent 212 are symmetrically disposed on two sides of the cavity channel 210. Certainly, in some other embodiments, the first connecting vent 211 and the second connecting vent 212 can be unsymmetrically disposed, it can be designed by one with ordinary skills in the art according to actual needs.
More specifically, relationship between the first connecting vent 211, the second connecting vent 212 and the third connecting vent 213 is set to be that: when the passage 110 moves to a predetermined position, that is, when the passage 110 moves to a position between the first connecting vent 211 and the second connecting vent 212, the first end of the passage 110 is connected to the first connecting vent 211, and the second  end of the passage 110 is connected to the second connecting vent 212, as shown in FIG. 4, such that gases can be easily driven in via the second connecting vent 212, and the target liquid in the passage 110 can be driven into the first connecting vent 211 due to air pressure difference.
On the above basis, as shown in FIG. 2, the third connecting vent 213 is disposed between the second connecting vent 212 and bottom of the cavity channel 210, and a first end of the air-passage 220 is connected to the second connecting vent 212, and a second end of the air-passage 220 is connected to the third connecting vent 213.
When the quantitative liquid-taking mechanism 10 is in actual use, the liquid carrying rod 100 is pushed to move toward the bottom of the cavity channel 210 from an entrance of the cavity channel 210, then gases in the cavity channel 210 are compressed, therefore the gases flow to the second connecting vent 212 through the third connecting vent 213, and then an air pressure difference generates between the second connecting vent 212 and the first connecting vent 211. On that basis, continue pushing the liquid carrying rod 100, until the second connecting vent 212 and the first connecting vent are connected to the passage 110 at the same time, under effect of the aforementioned air pressure difference, the target liquid in the passage 110 can be driven into the fluid-passage 230 through the first connecting vent 211.
Therefore, the quantitative liquid-taking mechanism 10 according to the first aspect of the present disclosure can do sampling work with measuring and taking liquid accurately through several simple structures, such as liquid carrying rod 100, cavity channel 210, air-passage 220 and fluid-passage 230. At the same time, the quantitative liquid-taking mechanism’s structural design is simple, and it is easy to manufacture with controlled manufacturing cost. Besides, these structures, such as liquid carrying rod 100, cavity channel 210, air-passage 220 and fluid-passage 230, are disposed on a base 200, which can further simplify the structural design.
Further, in some embodiments, as shown in FIG. 1, the cavity channel 210, the air-passage 220 and the fluid-passage 230 are all located in the base 200.
Further, in some embodiments, as shown in FIG. 2, a concave portion 260 is formed on the bottom of the cavity channel 210 and sunken towards interior of the cavity channel 210. Specifically, the concave portion 260 has a concave direction toward the entrance of the cavity channel 210. When the liquid carrying rod moves in the cavity channel 210, it is kept within a certain area by the concave portion, such that a certain space is always maintained between the liquid carrying rod 100 and bottom of  the cavity channel 210, as shown in FIG. 4. The function of the concave portion 260 is to maintain the connection of the cavity channel 210 and the air-passage 220 during the process of the liquid carrying rod 100 moving to bottom of the cavity channel 210 and to avoid the fluid carrying rod 100 being blocked in the cavity channel 210. Then compressed gas in the cavity channel 210 produces positive air pressure and would be continuously released through the air-passage 220, and liquid in the air-passage 220 or in the fluid-passage 230 is driven to flow into subsequent structures.
Further, in some embodiments, as shown in FIG. 1, 3 and 4, the quantitative liquid-taking mechanism 10 further includes a balancing hole 240 and a liquid-injecting hole 250, the balancing hole 240 and the liquid-injecting hole 250 are connected to the cavity channel 210 and are disposed at an area between the first connecting vent 211, the second connecting vent 212, and an entrance of the cavity channel 210; that is, when the liquid carrying rod 100 moves from the entrance of the cavity channel 210 toward the bottom of the cavity channel 210, it firstly passes through the balancing hole 240 and the liquid-injecting hole 250, and then passes through the first connecting vent 211 and the second connecting vent 212.
When the passage 110 moves to a second predetermined position, two ends of the passage 110 are connected to the balancing hole 240 and the liquid-injecting hole 250 respectively. That is, one end of the passage 110 is connected to the balancing hole 240, and the other end of the passage 110 is connected to the liquid-injecting hole 250, as shown in FIG. 3.
The main function of the balancing hole 240 and the liquid-injecting hole 250 is to add liquid into the passage 110 accurately, the specific operation can be as follows: sufficient amount of liquid is added to the liquid-injecting hole 250, and then, under effect of gravity and capillary siphon, the liquid flows from the liquid-injecting hole 250 to the balancing hole 240, and when the liquid stored on the liquid-injecting hole 250 and the balancing hole 240 reaches a state of balance, the cavity channel 110 is filled with measured liquid, so as to realize accuracy liquid-taking and liquid-adding work.
It should be noted that, the balancing hole 240 and the liquid-injecting hole 250 can be disposed on the base 200.
Further, in some embodiments, the balancing hole 240 and the liquid-injecting hole 250 are symmetrically disposed on two sides of the cavity channel 210.
<Second aspect: micro-fluidic device>
A micro-fluidic device is provided according to second aspect of the present disclosure, which includes:
a quantitative liquid-taking mechanism 10 according to the first aspect of the present disclosure; and
a micro-fluidic chip.
The micro-fluidic chip can be realized depend on existing available technology.
Since micro-fluidic chip has a high requirement to the accuracy of sample liquid, on the first hand, when the quantitative liquid-taking mechanism 10 mentioned in all embodiments according to the first aspect is used together with the micro-fluidic chip, a very good practical effect can be achieved in quantitative liquid adding.
Although embodiments of the present disclosure have been described in the above description, it is not limited to the listed in the described herein and embodiments, it can be applied to various fields which are suitable for the present disclosure, for one with ordinary skill in the art, it can easily achieve other modifications, therefore, the present disclosure is not limited to the specific details and illustrations that are shown and described here when without deviating from the general concept that is limited by the claims and their equivalents.

Claims (9)

  1. A quantitative liquid-taking mechanism, comprising:
    a liquid carrying rod having a passage formed therein and configured for quantitatively measuring and taking, and transporting a target liquid;
    a cavity channel sleeved on the liquid carrying rod and having a first connecting vent, a second connecting vent and a third connecting vent, wherein the third connecting vent is disposed between the second connecting vent and a bottom of the cavity channel, when the liquid carrying rod moves in the cavity channel to a predetermined position, one end of the passage is connected to the first connecting vent, and the other end of the passage is connected to the second connecting vent;
    an air-passage configured to connect the second connecting vent with the third connecting vent; and
    a fluid-passage configured for receiving and outputting the target liquid flowing from the first connecting vent.
  2. The quantitative liquid-taking mechanism according to claim 1, wherein the passage has a rectilinear shape.
  3. The quantitative liquid-taking mechanism according to claim 1, wherein a flow direction of the passage is vertical to a length direction of the liquid carrying rod.
  4. The quantitative liquid-taking mechanism according to claim 1, wherein the first connecting vent and the second connecting vent are symmetrically disposed on two sides of the cavity channel.
  5. The quantitative liquid-taking mechanism according to claim 1, wherein a concave portion is formed on the bottom of the cavity channel and sunken towards interior of the cavity channel.
  6. The quantitative liquid-taking mechanism according to claim 1, wherein the cavity channel, the air-passage and the fluid-passage are provided by a base.
  7. The quantitative liquid-taking mechanism according to claim 1, further comprising a  balancing hole and a liquid-injecting hole, wherein the balancing hole and the liquid-injecting hole are connected to the cavity channel and are disposed at an area between the first connecting vent, the second connecting vent and an entrance of the cavity channel;
    wherein when the passage moves to a second predetermined position, the two ends of the passage are connected to the balancing hole and the liquid-injecting hole respectively.
  8. The quantitative liquid-taking mechanism according to claim 7, wherein the balancing hole and the liquid-injecting hole are symmetrically disposed on two sides of the cavity channel.
  9. A micro-fluidic device, comprising:
    a quantitative liquid-taking mechanism according to claims 1; and
    a micro-fluidic chip.
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