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WO2020176599A1 - Azepino-indoles and other heterocycles for treating brain disorders - Google Patents

Azepino-indoles and other heterocycles for treating brain disorders Download PDF

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Publication number
WO2020176599A1
WO2020176599A1 PCT/US2020/019858 US2020019858W WO2020176599A1 WO 2020176599 A1 WO2020176599 A1 WO 2020176599A1 US 2020019858 W US2020019858 W US 2020019858W WO 2020176599 A1 WO2020176599 A1 WO 2020176599A1
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Prior art keywords
alkyl
heterocycloalkyl
cycloalkyl
compound
combined
Prior art date
Application number
PCT/US2020/019858
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French (fr)
Inventor
David E. Olson
Florence F. Wagner
Original Assignee
The Regents Of The University Of California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Priority to KR1020217030793A priority Critical patent/KR20210134689A/en
Application filed by The Regents Of The University Of California filed Critical The Regents Of The University Of California
Priority to JP2021549987A priority patent/JP2022521789A/en
Priority to SG11202109111VA priority patent/SG11202109111VA/en
Priority to AU2020229779A priority patent/AU2020229779A1/en
Priority to EP20763824.8A priority patent/EP3931189A4/en
Priority to BR112021016620A priority patent/BR112021016620A2/en
Priority to CA3130770A priority patent/CA3130770A1/en
Priority to MX2021010193A priority patent/MX2021010193A/en
Priority to CN202080017483.XA priority patent/CN113784962A/en
Publication of WO2020176599A1 publication Critical patent/WO2020176599A1/en
Priority to US17/345,745 priority patent/US11414423B1/en
Priority to IL285649A priority patent/IL285649A/en
Priority to US17/862,646 priority patent/US20230117791A1/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
    • C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
    • C07D487/04—Ortho-condensed systems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/55—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having seven-membered rings, e.g. azelastine, pentylenetetrazole
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A61P25/16—Anti-Parkinson drugs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/18—Antipsychotics, i.e. neuroleptics; Drugs for mania or schizophrenia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/24—Antidepressants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/30—Drugs for disorders of the nervous system for treating abuse or dependence
    • A61P25/32—Alcohol-abuse
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D453/00—Heterocyclic compounds containing quinuclidine or iso-quinuclidine ring systems, e.g. quinine alkaloids
    • C07D453/06—Heterocyclic compounds containing quinuclidine or iso-quinuclidine ring systems, e.g. quinine alkaloids containing isoquinuclidine ring systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D491/00—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00
    • C07D491/12—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00 in which the condensed system contains three hetero rings
    • C07D491/14—Ortho-condensed systems
    • C07D491/147—Ortho-condensed systems the condensed system containing one ring with oxygen as ring hetero atom and two rings with nitrogen as ring hetero atom

Definitions

  • the psychedelic alkaloid ibogaine has robust anti-addictive properties in the clinic and in animal models. Ibogaine possesses the potential to treat patients addicted to a variety of substances, for example, including opiates, psychostimulants, alcohol, and nicotine.
  • Ibogaine reduces symptoms of drug withdrawal, reduces drug cravings, and prevents relapse. In rodents, ibogaine reduces drug self-administration and prevents drug-induced dopamine release in several brain regions.
  • ibogaine reduces drug self-administration and prevents drug-induced dopamine release in several brain regions.
  • several safety concerns have hindered the clinical development of ibogaine, including, for example, its toxicity, hallucinogenic potential, and proclivity for inducing cardiac arrhythmias via hERG channel inhibition.
  • Ibogaine increases glial cell line-derived neurotrophic factor (GDNF) expression in the ventral tegmental area (VTA), and intra-VTA infusion of ibogaine reduces alcohol seeking behavior in rodents.
  • Ibogaine impacts brain-derived neurotrophic factor (BDNF) and GDNF signaling in multiple brain regions implicated in the pathophysiology of addiction.
  • BDNF brain-derived neurotrophic factor
  • Noribogaine, an active metabolite of ibogaine is a potent psychoplastogen capable of increasing cortical neuron dendritic arbor complexity.
  • psychoplastogens such as lysergic acid diethylamide (LSD) and psilocin (the active metabolite of psilocybin) have anti- addictive properties in the clinic similar to ibogaine.
  • LSD lysergic acid diethylamide
  • psilocin the active metabolite of psilocybin
  • the ability of psychoplastogens to promote structural and functional neural plasticity in addiction-related circuitry can explain their abilities to reduce drug-seeking behavior for weeks to months following a single administration.
  • a particular addictive substance e.g., opioid receptors, nicotinic receptors, etc.
  • psychoplastogens like ibogaine have the potential to be broadly applicable anti-addictive agents.
  • the present invention provides a compound of Formula I:
  • R 1 is hydrogen or Ci- 6 alkyl
  • R 3a and R 3b are each independently hydrogen, Ci- 6 alkyl, Cvx cycloalkyl, or C4-14 alkyl-cycloalkyl
  • R 3c is hydrogen or Ci-6 alkyl
  • the present invention provides a compound, or a pharmaceutically acceptable salt thereof, wherein the compound is
  • the present invention provides a pharmaceutical composition
  • a pharmaceutical composition comprising a compound of the present invention, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient.
  • the present invention provides a method for increasing neuronal plasticity, comprising contacting a neuronal cell with a compound of Formula I:
  • R 1 is hydrogen or Ci-6 alkyl
  • R 3a and R 3b are each independently hydrogen, Ci- 6 alkyl, C vs cycloalkyl, or C 4-14 alkyl-cycloalkyl
  • R 3c is hydrogen or Ci-6 alkyl
  • R 2 and R 3b are combined with the atoms to which they are attached to form a C 5-8 heterocycloalkyl substituted with 1 to 3 R 9 groups which are each independently hydrogen, Ci-6 alkyl, C3-8 cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10
  • the present invention provides a method of treating a brain disorder, comprising administering to a subject in need thereof a therapeutically effective amount of a compound of Formula I:
  • R 1 is hydrogen or Ci-6 alkyl
  • R 3a and R 3b are each independently hydrogen, Ci-6 alkyl, CYx cycloalkyl, or C 4-14 alkyl-cycloalkyl
  • R 3c is hydrogen or Ci- 6 alkyl
  • R 8b R 8c C(0)C(0)N (R 8b R 8c ), -S(0 2 )R 8b , -S(0) 2 N(R 8b R 8c ), C3- 8 cycloalkyl, C3-14 alkyl-cycloalkyl, C - 10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-I8 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, wherein at least one of R 4 , R 5 , R 6 and R 7 is not H; alternatively, R 4 and R 5 , R 5 and R 6 , or R 6 and R 7 are combined with the atoms to which they are each attached to form a C3-6 cycloalkyl, C3-6 heterocycloalkyl, Ce-n aryl or C5-10 heteroaryl; and R 8a , R 8b ,
  • R 8c and R 8d are each independently H, Ci-6 alkyl.
  • the present invention provides a method for increasing at least one of translation, transcription or secretion of neurotrophic factors, comprising contacting a neuronal cell with a compound of Formula I:
  • R 1 is hydrogen or Ci- 6 alkyl
  • R 3a and R 3b are each independently hydrogen, Ci- 6 alkyl, Cvs cycloalkyl, or C 4-14 alkyl-cycloalkyl
  • R 3c is hydrogen or Ci- 6 alkyl
  • R 2 and R 3b are combined with the atoms to which they are attached to form a C5-8 heterocycloalkyl substituted with 1 to 3 R 9 groups which are each independently hydrogen, Ci-6 alkyl, C3-8 cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10
  • R 8a , R 8b , R 8c and R 8d are each independently H, Ci- 6 alkyl.
  • FIG. 1A to FIG. IB show function-oriented synthesis of ibogalogs.
  • FIG. 1 A shows key structural features of ibogaine and related alkaloids. Deletion of either the isoquinuclidine or tetrahydroazepine leads to simplified ibogaine analogs.
  • FIG. IB shows synthesis of tetrahydroazepine-containing ibogalogs.
  • FIG. 2A to FIG.2K show tabemanthalog (TBG) is a safer analog of iboga alkaloids.
  • FIG. 2A shows the design of ibogainalog (IBG) and TBG, simplified analogs of ibogaine and tabemanthine, respectively.
  • FIG. 2C shows solubility of various drugs in a 0.9% saline solution.
  • FIG. 2D shows inhibition of hERG channels expressed in HEK293 cells.
  • FIG. 2E shows that unlike ibogaine, IBG and TBG do not induce bradycardia in larval zebrafish.
  • FIG. 2F shows ratio of atrium to ventricle beats per minute (BPM). Deviations from 1 indicate an increased risk of arrhythmias. Sertindole (SI) was used as a positive control.
  • FIG. 2G shows confusion matrix for classification of IBO, NOR, IBG, and TBG (200 mM), plus VEH and lethal controls. Cells show the proportion of classification between true labels (Y-axis) and predicted labels (X- axis).
  • FIG. 21 shows proportion of viable and non- viable (malformed or dead) zebrafish following treatment with compounds (100 pM).
  • FIG. 2J shows the effects of compound treatment on malformation and death over time.
  • FIG. 3A to FIG. 31 show TBG promotes neural plasticity.
  • FIG. 3B shows Sholl analysis of TBG and VEH treated neurons. The light shadings represent 95% confidence intervals.
  • FIG. 3C shows maximum numbers of crossings (Nmax) of the Sholl plot in b demonstrate that TBG increases dendritic arbor complexity.
  • FIG. 3D shows the effects of TBG on dendritic growth are blocked by the 5- HT2A antagonist ketanserin (KETSN).
  • FIG. 3E shows representative images of secondary branches of rat embryonic cortical neurons (DIV20) after treatment with ibogalogs for 24 h.
  • FIG. 3F shows TBG increases dendritic spine density on rat embryonic cortical neurons (DIV20) after treatment for 24 h.
  • KET ketamine.
  • FIG. 3G shows schematic illustrating the design of in vivo spine dynamics experiments using transcranial 2- photon imaging.
  • FIG. 3H shows representative images of the same dendritic segments from mouse primary sensory cortex before (Day 0) and after (Day 1) treatment with VEH, DOI, or TBG. Blue, red, and white arrowheads represent newly formed spines, eliminated spines, and filopodia, respectively.
  • Scale bar 2 pm.
  • FIG. 31 shows quantification of spine dynamics reveals that DOI and TBG increase spine formation but have no effect on spine elimination.
  • FIG. 4A to FIG. 4E show TBG has antidepressant and anti-addictive properties.
  • FIG. 4A shows schematic illustrating the design of FST experiments. Mice were subjected to a pretest, dosed with compounds, and then retested at 24 h and 7 days following drug administration.
  • FIG. 4B shows quantification of immobility reveals that TBG has antidepressant-like effects.
  • FIG. 4C shows timeline of binge-drinking experiment. Bottles of 20% EtOFI (white droplets) and FEO (blue droplets) were available for 24 h periods every 1- 2 days for 7 weeks. Between drinking sessions, two bottles of FEO were provided.
  • FIG. 4D shows TBG reduces EtOFI consumption and preference during a binge drinking session without impacting FEO intake.
  • FIG. 5A to FIG. 5B show the effects of ibogalogs on dendritogenesis.
  • FIG. 5B shows maximum numbers of crossings (Nmax) of the Sholl plots demonstrate that tetrahydroazepine-containing ibogalogs are more effective at increasing dendritic arbor complexity than are isoquinuclicine-containing ibogalogs.
  • FIG. 6 shows several ibogalogs promote dendritogenesis.
  • the shaded area surrounding each line represents 95% confidence intervals.
  • Control compounds, isoquinuclidines, and tetrahydroazepines are shown in blue, purple, and red, respectively.
  • FIG. 7 shows ibogaine hydrochloride exhibits limited solubility in various saline- based vehicles. Solutions of saline (0.9%) containing various percentages of co
  • solvents/additives were added to finely crushed ibogaine hydrochloride. All attempts to improve its solubility through pulverizing, sonication, and mild heating ( ⁇ 50 °C) were unsuccessful. Moreover, the addition of co-solvents (ethanol, dimethyl sulfoxide, glycerol), surfactants (Kolliphor), or hydrotropes (ATP) to the vehicle did not substantially improve its solubility. It was confirmed the purity and identity of the ibogaine hydrochloride used in these studies through a combination of NMR, LC-MS, and X-ray crystallography experiments.
  • FIG. 8 shows heatmaps representing aggregate larval zebraflsh locomotor activity per well compared to vehicle controls (pseudo-Z-score). Red and blue indicate higher and lower activity than the mean of vehicle controls, respectively, while white indicates activity within ⁇ 1 SD from control. Stimuli applied over time are indicated under the heatmaps. Colors indicate bright LED light of respective colors, black traces represent the waveforms of acoustic stimuli, and gray vertical lines indicate physical tapping as secondary acoustic stimuli.
  • FIG. 9 shows increasing concentrations of IBG and TBG do not produce movement responses in larval zebraflsh similar to a lethal concentration of eugenol (100 mM).
  • Concentration-response curves for compounds subjected to the battery of stimuli shown in FIG. 8. Response is inversely proportional to classification accuracy; 0-20% indicates no ability and 100% indicates perfect classification. Lower percentages indicate treatments that were more often classified as vehicle (blue) or lethal (red). The solid line denotes the median and the shading denotes a 95th percentile confidence interval calculated by bootstrap. N 8 wells / condition (64 animals / condition).
  • FIG. 10 shows TBG does not induce seizures in larval zebraflsh.
  • Transgenic larval zebraflsh expressing GCaMP5G were immobilized in agarose, treated with compounds, and imaged over time.
  • FIG. 1 1A and FIG. 1 IB show TBG (66 pM) does not cause developmental toxicity in zebrafish.
  • FIG. 12 shows concentration-response curves demonstrating the abilities of ibogalogs and related compounds to activate 5-HT and opioid receptors. All compounds were assayed in parallel using the same drug dilutions. Graphs reflect representative
  • FIG. 14 shows screening of DMT derivatives and ibogalogs reveals differences in 5-HT receptorome profiles. Ibogalogs are more selective 5-HT 2A agonists than 5-MeO-DMT.
  • FIG. 15 shows the dose response profile of hallucinogenic and non-hallucinogenic compounds to a 5HT 2A sensor assay in agonist mode.
  • FIG. 16 shows the response profile of hallucinogenic and non-hallucinogenic compounds to a 5HT 2A sensor assay in agonist mode.
  • FIG. 17 shows the dose response profile of 5HT, 6-MeO-DMT (FIG. 17A), and lisuride (FIG. 17B) to a 5HT2 A sensor assay in antagonist mode.
  • FIG. 18 shows the response profile of hallucinogenic and non-hallucinogenic compounds to a 5HT 2A sensor assay in antagonist mode.
  • Ibogaine has garnered attention as a potential plasticity-promoting anti-addictive agent; a single dose of this hallucinogenic compound has demonstrated sustained efficacy for treating addiction to alcohol, opiates, nicotine, and psychostimulants.
  • An analog of ibogaine capable of promoting fibroblast growth factor 2 (FGF2)-induced GDNF release in C6 glioma cells was developed; however, the effects of this compound on neural plasticity are not known.
  • FGF2 fibroblast growth factor 2
  • Ibogaine is highly plasticity-promoting.
  • ibogaine s hallucinogenic effects present barriers to regulatory approval and severely limits its therapeutic potential. It is possible to decouple the hallucinogenic effects of a drug from its ability to promote neural plasticity.
  • Compounds capable of modifying neural circuits that control motivation, anxiety, and drug-seeking behavior have potential for treating depression, post-traumatic stress disorder (PTSD), and substance abuse disorder (SUD). In some instances, such
  • psychoplastogenic medicines produce sustained therapeutic effects because the medicines treat the underlying pathological changes in circuitry.
  • Psychedelic compounds have distinguished themselves in this regard because, for example, they promote structural and functional neural plasticity in key circuits, elicit therapeutic responses in multiple neuropsychiatric disorders, and produce beneficial effects that last for months following a single administration.
  • 5-HT 2A agonists e.g., DMT, LSD, DOI, etc.
  • abrogate the neuritogenesis and spinogenesis effects of hallucinogenic compounds with 5-HT2 A agonist activity e.g., DMT, LSD, and DOI, demonstrating the correlation of 5-HT2 A agonism and the promotion of neural plasticity (Ly et al., 2018; Dunlap et al., 2020).
  • non-hallucinogenic psychoplastogens are provided herein in some embodiments.
  • the non-hallucinogenic ibogaine analogs described herein have improved physiochemical properties as a result of, for example, decreasing total polar surface area.
  • Described herein in some embodiments are non-hallucinogenic compounds that demonstrate similar therapeutic potential as hallucinogenic 5-HT 2A agonists.
  • the non-hallucinogenic compounds described herein provide better therapeutic potential than hallucinogenic 5-HT 2A modulators for neurological diseases.
  • the present invention provides azepino-indoles and other heterocyclic non- hallucinogenic compounds useful for the treatment of a variety of brain disorders and other conditions, as well as increasing neuronal plasticity, or increasing at least one of translation, transcription, or secretion of neurotrophic factors.
  • “A,”“an,” or“the” not only include aspects with one member, but also include aspects with more than one member.
  • the singular forms“a,”“an,” and“the” include plural referents unless the context clearly dictates otherwise.
  • reference to“a cell” includes a plurality of such cells and reference to“the agent” includes reference to one or more agents known to those skilled in the art, and so forth.
  • Alkyl refers to a straight or branched, saturated, aliphatic radical having the number of carbon atoms indicated. Alkyl can include any number of carbons, such as C1-2, Cl-3, Cl-4, Cl-5, Cl-6, Cl-7, Cl-8, Cl-9, Cl-10, C2-3, C2-4, C2-5, C2-6, C3-4, C3-5, C3-6, C4-5, C4-6 and C5-6.
  • Ci- 6 alkyl includes, but is not limited to, methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec-butyl, tert-butyl, pentyl, isopentyl, hexyl, etc.
  • Alkyl can also refer to alkyl groups having up to 20 carbons atoms, such as, but not limited to heptyl, octyl, nonyl, decyl, etc. Alkyl groups can be substituted or unsubstituted.
  • Alkylene refers to a straight or branched, saturated, aliphatic radical having the number of carbon atoms indicated, and linking at least two other groups, i.e., a divalent hydrocarbon radical.
  • the two moieties linked to the alkylene can be linked to the same atom or different atoms of the alkylene group.
  • a straight chain alkylene can be the bivalent radical of -(CFE , where n is 1, 2, 3, 4, 5 or 6.
  • Representative alkylene groups include, but are not limited to, methylene, ethylene, propylene, isopropylene, butylene, isobutylene, sec-butylene, pentylene and hexylene.
  • Alkylene groups can be substituted or unsubstituted.
  • Alkenyl refers to a straight chain or branched hydrocarbon having at least 2 carbon atoms and at least one double bond. Alkenyl can include any number of carbons, such as C2, C2-3, C2-4, C2-5, C2-6, C2-7, C2-8, C2-9, C2-10, C3, C3-4, C3-5, C3-6, C4, C4-5, C4-6, C5, C5-6, and Ce. Alkenyl groups can have any suitable number of double bonds, including, but not limited to, 1, 2, 3, 4, 5 or more.
  • alkenyl groups include, but are not limited to, vinyl (ethenyl), propenyl, isopropenyl, 1-butenyl, 2-butenyl, isobutenyl, butadienyl, 1-pentenyl, 2-pentenyl, isopentenyl, 1,3-pentadienyl, 1,4-pentadienyl, 1-hexenyl, 2-hexenyl, 3-hexenyl, 1,3-hexadienyl, 1 ,4-hexadienyl, 1,5-hexadienyl, 2,4-hexadienyl, or
  • Alkenyl groups can be substituted or unsubstituted.
  • Alkynyl refers to either a straight chain or branched hydrocarbon having at least 2 carbon atoms and at least one triple bond. Alkynyl can include any number of carbons, such as C2, C2-3, C2-4, C2-5, C2-6, C2-7, C2-8, C2-9, C2-10, C3, C3-4, C3-5, C3-6, C4, C4-5, C4-6, C5, C5-6, and Ce.
  • alkynyl groups include, but are not limited to, acetylenyl, propynyl, 1-butynyl, 2-butynyl, butadiynyl, 1-pentynyl, 2-pentynyl, isopentynyl, 1,3-pentadiynyl,
  • Alkynyl groups can be substituted or unsubstituted.
  • Cycloalkyl refers to a saturated or partially unsaturated, monocyclic, bicyclic, fused bicyclic or bridged polycyclic ring assembly containing from 3 to 12 ring atoms, or the number of atoms indicated. Cycloalkyl can include any number of carbons, such as C3-6,
  • Saturated monocyclic cycloalkyl rings include, for example, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, and cyclooctyl.
  • Bicyclic compounds include spirocyclic compounds, fused bicyclic compounds and bridged bicyclic compounds.
  • Saturated bicyclic and polycyclic cycloalkyl rings include, for example, norbomane, [2.2.2] bicyclooctane, decahydronaphthalene and adamantane.
  • Cycloalkyl groups can also be partially unsaturated, having one or more double or triple bonds in the ring.
  • Representative cycloalkyl groups that are partially unsaturated include, but are not limited to, cyclobutene, cyclopentene, cyclohexene, cyclohexadiene (1,3- and 1,4-isomers), cycloheptene, cycloheptadiene, cyclooctene, cyclooctadiene (1,3-, 1,4- and 1,5-isomers), norbomene, and norbomadiene.
  • exemplary groups include, but are not limited to cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl and cyclooctyl.
  • exemplary groups include, but are not limited to cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl. Cycloalkyl groups can be substituted or unsubstituted.
  • Alkyl-cycloalkyl refers to a radical having an alkyl component and a cycloalkyl component, where the alkyl component links the cycloalkyl component to the point of attachment.
  • the alkyl component is as defined above, except that the alkyl component is at least divalent, an alkylene, to link to the cycloalkyl component and to the point of attachment. In some instances, the alkyl component can be absent.
  • the alkyl component can include any number of carbons, such as Ci-6, C1-2, C1-3, CM, C1-5, C2-3, C2-4, C2-5, C2-6, C3-4, C3-5, C3-6,
  • cycloalkyl component is as defined within.
  • exemplary alkyl- cycloalkyl groups include, but are not limited to, methyl-cyclopropyl, methyl-cyclobutyl, methyl-cyclopentyl and methyl-cyclohexyl.
  • Heterocycloalkyl refers to a cycloalkyl as defined above, having from 3 to 12 ring members and from 1 to 4 heteroatoms of N, O and S.
  • Heterocycloalkyl includes bicyclic compounds which include a heteroatom.
  • Bicyclic compounds includes spirocyclic compounds, fused bicyclic compounds, and bridged bicyclic compounds
  • the heteroatoms can also be oxidized, such as, but not limited to, -S(O)- and -S(0) 2 -.
  • Heterocycloalkyl groups can include any number of ring atoms, such as, 3 to 6, 4 to 6, 5 to 6, 3 to 8, 4 to 8, 5 to 8,
  • heterocycloalkyl groups such as 1, 2, 3, or 4, or 1 to 2, 1 to 3, 1 to 4, 2 to 3, 2 to 4, or 3 to 4.
  • the heterocycloalkyl group can include groups such as aziridine, azetidine, pyrrolidine, piperidine, azepane, azocane, quinuclidine, pyrazolidine,
  • heterocycloalkyl groups can also be fused to aromatic or non-aromatic ring systems to form members including, but not limited to, indoline.
  • Heterocycloalkyl groups can be unsubstituted or substituted.
  • Alkyl-heterocycloalkyl refers to a radical having an alkyl component and a heterocycloalkyl component, where the alkyl component links the heterocycloalkyl component to the point of attachment.
  • the alkyl component is as defined above, except that the alkyl component is at least divalent, an alkylene, to link to the heterocycloalkyl component and to the point of attachment.
  • the alkyl component can include any number of carbons, such as Co-6, C1-2, C1-3, CM, C1-5, Ci-6, C2-3, C2-4, C2-5, C2-6, C3-4, C3-5, C3-6, C4-5, C4-6 and C5-6. In some instances, the alkyl component can be absent.
  • the heterocycloalkyl component is as defined above. Alkyl-heterocycloalkyl groups can be substituted or unsubstituted.
  • Halogen refers to fluorine, chlorine, bromine and iodine.
  • Haloalkyl refers to alkyl, as defined above, where some or all of the hydrogen atoms are replaced with halogen atoms.
  • alkyl group haloalkyl groups can have any suitable number of carbon atoms, such as Ci- 6 .
  • haloalkyl includes
  • perfluoro can be used to define a compound or radical where all the hydrogens are replaced with fluorine.
  • perfluoromethyl refers to 1,1,1 -trifluoromethyl.
  • Alkoxy refers to an alkyl group having an oxygen atom that connects the alkyl group to the point of attachment: alkyl-O-.
  • alkyl group alkoxy groups can have any suitable number of carbon atoms, such as Ci- 6 .
  • Alkoxy groups include, for example, methoxy, ethoxy, propoxy, iso-propoxy, butoxy, 2-butoxy, iso-butoxy, sec-butoxy, tert-butoxy, pentoxy, hexoxy, etc.
  • the alkoxy groups can be further substituted with a variety of substituents described within. Alkoxy groups can be substituted or unsubstituted.
  • Haloalkoxy refers to an alkoxy group where some or all of the hydrogen atoms are substituted with halogen atoms.
  • haloalkoxy groups can have any suitable number of carbon atoms, such as Ci-6.
  • the alkoxy groups can be substituted with 1, 2, 3, or more halogens. When all the hydrogens are replaced with a halogen, for example by fluorine, the compounds are per-substituted, for example, perfluorinated.
  • Haloalkoxy includes, but is not limited to, trifluoromethoxy, 2,2,2,-trifluoroethoxy, perfluoroethoxy, etc.
  • “Amine” refers to an -N(R)2 group where the R groups can be hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl, among others.
  • the R groups can be the same or different.
  • the amino groups can be primary (each R is hydrogen), secondary (one R is hydrogen) or tertiary (each R is other than hydrogen).
  • Alkyl amine refers to an alkyl group as defined within, having one or more amino groups.
  • the amino groups can be primary, secondary or tertiary.
  • the alkyl amine can be further substituted with a hydroxy group to form an amino-hydroxy group.
  • Alkyl amines useful in the present invention include, but are not limited to, ethyl amine, propyl amine, isopropyl amine, ethylene diamine and ethanolamine.
  • the amino group can link the alkyl amine to the point of attachment with the rest of the compound, be at the omega position of the alkyl group, or link together at least two carbon atoms of the alkyl group.
  • alkyl amines are useful in the present invention.
  • Aryl refers to an aromatic ring system having any suitable number of ring atoms and any suitable number of rings.
  • Aryl groups can include any suitable number of ring atoms, such as, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 or 16 ring atoms, as well as from 6 to 10, 6 to 12, or 6 to 14 ring members.
  • Aryl groups can be monocyclic, fused to form bicyclic or tricyclic groups, or linked by a bond to form a biaryl group.
  • Representative aryl groups include phenyl, naphthyl and biphenyl. Other aryl groups include benzyl, having a methylene linking group.
  • aryl groups have from 6 to 12 ring members, such as phenyl, naphthyl or biphenyl. Other aryl groups have from 6 to 10 ring members, such as phenyl or naphthyl. Some other aryl groups have 6 ring members, such as phenyl.
  • Aryl groups can be substituted or unsubstituted.
  • Alkyl-aryl refers to a radical having an alkyl component and an aryl component, where the alkyl component links the aryl component to the point of attachment.
  • the alkyl component is as defined above, except that the alkyl component is at least divalent, an alkylene, to link to the aryl component and to the point of attachment.
  • the alkyl component can include any number of carbons, such as Co-6, C1-2, Cm, CM, CM, CM, C2-3, C2-4, C2-5, C2-6, C3-4, C3-5, C3-6, C4-5, C4-6 and C5-6. In some instances, the alkyl component can be absent.
  • the aryl component is as defined above. Examples of alkyl-aryl groups include, but are not limited to, benzyl and ethyl-benzene. Alkyl-aryl groups can be substituted or unsubstituted.
  • Heteroaryl refers to a monocyclic or fused bicyclic or tricyclic aromatic ring assembly containing 5 to 16 ring atoms, where from 1 to 5 of the ring atoms are a heteroatom such as N, O or S. Heteroaryl groups can include any number of ring atoms, such as, 5 to 6,
  • heteroaryl groups can have from 5 to 8 ring members and from 1 to 4 heteroatoms, or from 5 to 8 ring members and from 1 to 3 heteroatoms, or from 5 to 6 ring members and from 1 to 4 heteroatoms, or from 5 to 6 ring members and from 1 to 3 heteroatoms.
  • the heteroaryl group can include groups such as pyrrole, pyridine, imidazole, pyrazole, triazole, tetrazole, pyrazine, pyrimidine, pyridazine, triazine (1,2,3-, 1,2,4- and 1,3,5-isomers), thiophene, furan, thiazole, isothiazole, oxazole, and isoxazole.
  • heteroaryl groups can also be fused to aromatic ring systems, such as a phenyl ring, to form members including, but not limited to, benzopyrroles such as indole and isoindole, benzopyridines such as quinoline and isoquinoline, benzopyrazine (quinoxaline), benzopyrimidine (quinazoline), benzopyridazines such as phthalazine and cinnoline, benzothiophene, and benzofuran.
  • Other heteroaryl groups include heteroaryl rings linked by a bond, such as bipyridine. Heteroaryl groups can be substituted or unsubstituted.
  • Alkyl-heteroaryl refers to a radical having an alkyl component and a heteroaryl component, where the alkyl component links the heteroaryl component to the point of attachment.
  • the alkyl component is as defined above, except that the alkyl component is at least divalent, an alkylene, to link to the heteroaryl component and to the point of attachment.
  • the alkyl component can include any number of carbons, such as Co-6, C1-2, C1-3, CM, C1-5, Ci-6, C2-3, C2-4, C2-5, C2-6, C3-4, C3-5, C3-6, C4-5, C4-6 and C5-6. In some instances, the alkyl component can be absent.
  • the heteroaryl component is as defined within. Alkyl-heteroaryl groups can be substituted or unsubstituted.
  • Salt refers to acid or base salts of the compounds used in the methods of the present invention.
  • Illustrative examples of pharmaceutically acceptable salts are mineral acid (hydrochloric acid, hydrobromic acid, phosphoric acid, and the like) salts, organic acid (fumaric acid, acetic acid, propionic acid, glutamic acid, citric acid and the like) salts, quaternary ammonium (methyl iodide, ethyl iodide, and the like) salts. It is understood that the pharmaceutically acceptable salts are non-toxic. Additional information on suitable pharmaceutically acceptable salts can be found in Remington's Pharmaceutical Sciences, 17th ed., Mack Publishing Company, Easton, Pa., 1985, which is incorporated herein by reference.
  • the neutral forms of the compounds may be regenerated by contacting the salt with a base or acid and isolating the parent compound in the conventional manner.
  • the parent form of the compound differs from the various salt forms in certain physical properties, such as solubility in polar solvents, but otherwise the salts are equivalent to the parent form of the compound for the purposes of the present invention.
  • “Pharmaceutically acceptable salt” refers to a compound in salt form, wherein the compound are suitable for administration to a subject.
  • Representative pharmaceutically acceptable salts include salts of acetic, ascorbic, benzenesulfonic, benzoic, camphorsulfonic, citric, ethanesulfonic, edisylic, fumaric, gentisic, gluconic, glucoronic, glutamic, hippuric, hydrobromic, hydrochloric, isethionic, lactic, lactobionic, maleic, malic, mandelic, methanesulfonic, mucic, naphthalenesulfonic, naphthalene- 1,5-disulfonic, naphthalene-2, 6- disulfonic, nicotinic, nitric, orotic, pamoic, pantothenic, phosphoric, succinic, sulfuric, tartaric, p-toluenesulfonic and xinafoic acid, and the like
  • “Pharmaceutically acceptable excipient” refers to a substance that aids the administration of an active agent to and absorption by a subject.
  • Pharmaceutical excipients useful in the present invention include, but are not limited to, binders, fillers, disintegrants, lubricants, coatings, sweeteners, flavors and colors.
  • binders include, but are not limited to, binders, fillers, disintegrants, lubricants, coatings, sweeteners, flavors and colors.
  • composition refers to a product comprising the specified ingredients in the specified amounts, as well as any product, which results, directly or indirectly, from combination of the specified ingredients in the specified amounts.
  • pharmaceutically acceptable it is meant the carrier, diluent or excipient must be compatible with the other ingredients of the formulation.
  • Isomers refers to compounds with same chemical formula but different connectivity between the atoms in the molecule, leading to distinct chemical structures. Isomers include structural isomers and stereoisomers. Examples of structural isomers include, but are not limited to tautomers and regioisomers. Examples of stereoisomers include but are not limited to diastereomers and enantiomers.
  • administering refers to oral administration, administration as a suppository, topical contact, parenteral, intravenous, intraperitoneal, intramuscular, intralesional, intranasal or subcutaneous administration, intrathecal administration, or the implantation of a slow-release device e.g., a mini-osmotic pump, to the subject.
  • a slow-release device e.g., a mini-osmotic pump
  • Subject refers to animals such as mammals, including, but not limited to, primates (e.g., humans), cows, sheep, goats, horses, dogs, cats, rabbits, rats, mice and the like. In certain embodiments, the subject is a human.
  • “Therapeutically effective amount” or“therapeutically sufficient amount” or “effective or sufficient amount” refers to a dose that produces therapeutic effects for which it is administered. The exact dose will depend on the purpose of the treatment, and will be ascertainable by one skilled in the art using known techniques (see, e.g. , Lieberman, Pharmaceutical Dosage Forms (vols.
  • the therapeutically effective dose can often be lower than the conventional therapeutically effective dose for non- sensitized cells.
  • Neuronal plasticity refers to the ability of the brain to change its structure and/or function continuously throughout a subject’s life. Examples of the changes to the brain include, but are not limited to, the ability to adapt or respond to internal and/or external stimuli, such as due to an injury, and the ability to produce new neurites, dendritic spines, and synapses.
  • Brain disorder refers to a neurological disorder which affects the brain’s structure and function.
  • Brain disorders can include, but are not limited to, Alzheimer’s, Parkinson’s disease, psychological disorder, depression, treatment resistant depression, addiction, anxiety, post-traumatic stress disorder, suicidal ideation, major depressive disorder, bipolar disorder, schizophrenia, stroke, traumatic brain injury, and substance use disorder.
  • Combination therapy refers to a method of treating a disease or disorder, wherein two or more different pharmaceutical agents are administered in overlapping regimens so that the subject is simultaneously exposed to both agents.
  • the compounds of the invention can be used in combination with other pharmaceutically active compounds.
  • the compounds of the invention can be administered simultaneously (as a single preparation or separate preparation) or sequentially to the other drug therapy.
  • a combination therapy envisions administration of two or more drugs during a single cycle or course of therapy.
  • Neurotrophic factors refers to a family of soluble peptides or proteins which support the survival, growth, and differentiation of developing and mature neurons.
  • Modulate or“modulating” or“modulation” refers to an increase or decrease in the amount, quality, or effect of a particular activity, function or molecule.
  • agonists, partial agonists, antagonists, and allosteric modulators e.g., a positive allosteric modulator
  • a G protein-coupled receptor e.g., 5HT2 A
  • Agonism refers to the activation of a receptor or enzyme by a modulator, or agonist, to produce a biological response.
  • “Agonist” refers to a modulator that binds to a receptor or enzyme and activates the receptor to produce a biological response.
  • “5HT2 A agonist” can be used to refer to a compound that exhibits an EC50 with respect to 5HT2 A activity of no more than about 100 mM.
  • “agonist” includes full agonists or partial agonists.
  • “Full agonist” refers to a modulator that binds to and activates a receptor with the maximum response that an agonist can elicit at the receptor.“Partial agonist” refers to a modulator that binds to and activates a given receptor, but has partial efficacy, that is, less than the maximal response, at the receptor relative to a full agonist.
  • “Positive allosteric modulator” refers to a modulator that binds to a site distinct from the orthosteric binding site and enhances or amplifies the effect of an agonist.
  • Antagonist refers to the inactivation of a receptor or enzyme by a modulator, or antagonist.
  • Antagonism of a receptor for example, is when a molecule binds to the receptor and does not allow activity to occur.
  • Antagonist or“neutral antagonist” refers to a modulator that binds to a receptor or enzyme and blocks a biological response.
  • An antagonist has no activity in the absence of an agonist or inverse agonist but can block the activity of either, causing no change in the biological response.
  • IC50 refers to the concentration of a substance (e.g., a compound or a drug) that is required for 50% inhibition of a biological process.
  • IC50 refers to the half maximal (50%) inhibitory concentration (IC) of a substance as determined in a suitable assay.
  • an IC50 is determined in an in vitro assay system.
  • IC50 refers to the concentration of a modulator (e.g., an antagonist or inhibitor) that is required for 50% inhibition of a receptor, for example, 5HT2 A .
  • azepino-indoles and other heterocyclic non- hallucinogenic compounds e.g., Formula (I) or Formula (la)
  • azepino-indoles and other heterocyclic compounds provided herein are 5-HT2 A modulators and promote neural plasticity (e.g., cortical structural plasticity).
  • the present invention provides a compound of Formula I:
  • R 1 is hydrogen or Ci-6 alkyl
  • R 3a and R 3b are each independently hydrogen, Ci-6 alkyl, Cvx cycloalkyl, or C4-14 alkyl-cycloalkyl
  • R 3c is hydrogen or Ci- 6 alkyl
  • heterocycloalkyl and R 6 is Ci- 6 alkyl, halogen, Ci- 6 alkyoxy, -C(0)FI, -NFI2, then at least one of R 4 , R 5 , and R 7 is not hydrogen; and wherein when R 2 and R 3b are combined with the atoms to which they are attached to form a CV, heterocycloalkyl, and R 6 is methyl, halogen, or - C(0)H, then at least one of R 4 , R 5 , and R 7 is not methyl or Cl, or pharmaceutically acceptable salts and isomers thereof.
  • the present invention provides a compound of Formula I
  • R 1 is hydrogen or Ci- 6 alkyl
  • R 3a and R 3b are each independently hydrogen, Ci- 6 alkyl, Cvx cycloalkyl, or C4-14 alkyl-cycloalkyl
  • R 3c is hydrogen or Ci-6 alkyl
  • R 1 is hydrogen or Ci- 6 alkyl. In some embodiments, R 1 is hydrogen, methyl, ethyl, or propyl. In some embodiments, R 1 is hydrogen or methyl. In some embodiments, R 1 is hydrogen. In some embodiments, R 1 is methyl. In some embodiments, the present invention provides a compound, or a pharmaceutically acceptable salt thereof, wherein R 1 is hydrogen or methyl. In some embodiments, the present invention provides a compound, or a pharmaceutically acceptable salt thereof, wherein R 1 is hydrogen.
  • R 3a is hydrogen, Ci- 6 alkyl, CYx cycloalkyl, or C 4-14 alkyl- cycloalkyl. In some embodiments, R 3a is hydrogen or Ci- 6 alkyl. In some embodiments, R 3a is hydrogen, methyl, ethyl, or propyl. In some embodiments, R 3a is hydrogen or methyl. In some embodiments R 3a is methyl. In some embodiments, R 3a is hydrogen. In some embodiments, the present invention provides a compound, or a pharmaceutically acceptable salt thereof, wherein R 3a is hydrogen or Ci- 6 alkyl. In some embodiments, the present invention provides a compound, or a pharmaceutically acceptable salt thereof, wherein R 3a is hydrogen or methyl.
  • R 4 , R 5 , R 6 and R 7 are each independently hydrogen, Ci- 6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, halogen, Ci- 6 haloalkyl, Ci- 6 alkylamine, Ci- 6 alkoxy, Ci- 6 haloalkoxy, -OR 8a , -NO2, -CN, -C(0)R 8b , -C(0)OR 8b , -OC(0)R 8b , -OC(0)OR 8b , -N(R 8b R 8c ), cycloalkyl, C 3 - 14 alkyl-cycloalkyl, C 4-10 heterocycloalkyl, C 4-16 alkyl-heterocycloalkyl, C 6-12 aryl, C 7-18 alkyl- aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, wherein at least one of R 4 , R 5 , R
  • the present invention provides a compound, or a
  • R 4 , R 5 , R 6 and R 7 are each independently hydrogen, Ci- 6 alkyl, halogen, Ci- 6 haloalkyl, Ci- 6 alkoxy, Ci- 6 haloalkoxy, -OR 8a , -NO 2 , - CN, -N(R 8b R 8c ), -N(R 8b )C(0)R 8c , -C(0)N(R 8b R 8c ), CYs cycloalkyl, C 3-14 alkyl-cycloalkyl, C 4 - 10 heterocycloalkyl, or C 4-16 alkyl-heterocycloalkyl, wherein at least one of R 4 , R 5 , R 6 and R 7 is not H; alternatively, R 4 and R 5 , R 5 and R 6 , or R 6 and R 7 are combined with the atoms to which they are each attached to form a C3-6 heterocycloalkyl; and R 8a ,
  • the present invention provides a compound, or a
  • R 6 is Ci-6 alkyl, halogen, Ci-6 haloalkyl, Ci- 6 alkoxy, Ci-b haloalkoxy, -OR 8a , -NO 2 , -CN, -N(R 8b R 8c ), -N(R 8b )C(0)R 8c , -C(0)N(R 8b R 8c ), C 3-8 cycloalkyl, C 3-14 alkyl-cycloalkyl, C 4-10 heterocycloalkyl, or C 4-16 alkyl-heterocycloalkyl; alternatively, R 5 and R 6 are combined with the atoms to which they are each attached to form a C 3-6 heterocycloalkyl; and R 8a , R 8b andR 8c are each independently H, Ci- 6 alkyl, or C 3-6 cycloalkyl.
  • the present invention provides a compound, or a
  • R 4 , R 5 , R 6 and R 7 are each independently hydrogen, Ci- 6 alkyl, halogen, Ci- 6 haloalkyl, Ci- 6 alkoxy, Ci- 6 haloalkoxy, -OR 8a , - N(R 8b R 8c ), -N(R 8b )C(0)R 8c , C4-10 heterocycloalkyl, or C4-16 alkyl-heterocycloalkyl, wherein at least one of R 4 , R 5 , R 6 and R 7 is not H; alternatively, R 5 and R 6 are combined with the atoms to which they are each attached to form a C3-6 heterocycloalkyl; and R 8a , R 8b and R 8c are each independently H, Ci-6 alkyl, or C3-6 cycloalkyl.
  • the present invention provides a compound, or a
  • R 6 is Ci- 6 alkyl, halogen, Ci- 6 haloalkyl, Ci-6 alkoxy, Ci-6 haloalkoxy, -OR 8a , -N(R 8b R 8c ), -N(R 8b )C(0)R 8c , C4-10 heterocycloalkyl, or C4-16 alkyl-heterocycloalkyl; alternatively, R 5 and R 6 are combined with the atoms to which they are each attached to form a C3-6 heterocycloalkyl; and R 8a , R 8b and R 8c are each independently H, Ci- 6 alkyl, or C3-6 cycloalkyl.
  • the present invention provides a compound, or a
  • R 4 , R 5 , R 6 and R 7 are each independently hydrogen, Ci-6 alkyl, halogen, Ci-6 haloalkyl, Ci-6 alkoxy, Ci-6 haloalkoxy, -OR 8a , - N(R 8b R 8c ), or -N(R 8b )C(0)R 8c wherein at least one of R 4 , R 5 , R 6 and R 7 is not H; R 8a is H; and R 8b and R 8c are each independently H or -Me; alternatively, R 5 and R 6 are combined with the atoms to which they are each attached to form a C3-6 heterocycloalkyl.
  • the present invention provides a compound, wherein R 4 , R 5 , R 6 and R 7 are each independently hydrogen, Ci- 6 alkyl, halogen, Ci- 6 haloalkyl, Ci- 6 alkoxy, Ci-6 haloalkoxy, or -OR 8a , wherein at least one of R 4 , R 5 , R 6 and R 7 is not H; and R 8a is H; alternatively, R 5 and R 6 can be combined with the atoms to which they are each attached to form a C3-6 heterocycloalkyl.
  • the present invention provides a compound, or a
  • R 4 , R 5 , R 6 and R 7 are each independently H, F, Cl, Br, -OH, -OMe, -CF 3 -OCF3, -Me, -NMe 2 , -NHC(0)Me, or -N(Me)C(0)Me, wherein at least one of R 4 , R 5 , R 6 and R 7 is not H; alternatively, R 5 and R 6 are combined to form a 1,3-dioxole ring, or 1,4-dioxane ring.
  • the present invention provides a compound, or a
  • R 4 is H, F, -Me, -CF3, -OCF3, or -OMe
  • R 5 is H, F, Cl, Br, -Me, -CF3, -OCF3, -OH or -OMe
  • R 6 is H, F, -OH, -OMe, -OiPr, -Me, -CF3, - OCF3, -NMC2, -NHC(0)Me, or -N(Me)C(0)Me
  • R 5 and R 6 are combined to form a 1,3-dioxole ring or 1,4-dioxane ring
  • R 7 is H, wherein at least one of R 4 , R 5 and R 6 is not H.
  • the present invention provides a compound, wherein R 4 , R 5 , R 6 and R 7 are each independently H, F, Cl, Br, -OH, -OMe, -CF3 or -OCF3, wherein at least one of R 4 , R 5 , R 6 and R 7 is not H; alternatively, R 5 and R 6 are combined to form a 1,3-dioxole ring.
  • the present invention provides a compound, wherein R 4 is H, F, or -OMe; R 5 is H, F, Cl, Br, -OH or -OMe; R 6 is H, F, -OMe, -CF3 or -OCF3; alternatively, R 5 and R 6 are combined to form a 1,3-dioxole ring; and R 7 is H, wherein at least one of R 4 , R 5 , R 6 and R 7 is not H.
  • the present invention provides a compound, or a
  • R 6 is F, -OH, -OMe, -OiPr, -Me, -CF3, - OCF3, -NMC2, -NHC(0)Me, or -N(Me)C(0)Me; alternatively, R 5 and R 6 are combined to form a 1,3-dioxole ring or 1,4-dioxane ring.
  • R 4 and R 5 , R 5 and R 6 , or R 6 and R 7 are combined with the atoms to which they are each attached to form a C3-6 cycloalkyl, C3-6 heterocycloalkyl, C6-12 aryl or C5-10 heteroaryl. In some embodiments, R 4 and R 5 , R 5 and R 6 , or R 6 and R 7 are combined with the atoms to which they are each attached to form a C3-6 cycloalkyl or C3-6 heterocycloalkyl. In some embodiments, R 4 and R 5 are combined with the atoms to which they are each attached to form a C3-6 heterocycloalkyl.
  • R 5 and R 6 are combined with the atoms to which they are each attached to form a C3-6 heterocycloalkyl. In some embodiments, R 6 and R 7 are combined with the atoms to which they are each attached to form a C3-6 heterocycloalkyl. In some embodiments, R 5 and R 6 are combined to form a 1,3-dioxole ring or 1,4-dioxane ring.
  • the present invention provides a compound, or a pharmaceutically acceptable salt thereof, wherein R 2 and R 3b are combined with the atoms to which they are attached to form a C 5-8 heterocycloalkyl. In some embodiments, the present invention provides a compound, or a pharmaceutically acceptable salt thereof, wherein R 2 and R 3b are combined with the atoms to which they are attached to form a C 7- 8 heterocycloalkyl.
  • the present invention provides a compound, or a
  • R 6 is Ci- 6 alkyl, C 2-6 alkenyl, C 2-6 alkynyl, halogen, Ci- 6 haloalkyl, Ci- 6 alkylamine, Ci-6 alkoxy, Ci-b haloalkoxy, -OR 8a , -NO2, -CN, -C(0)R 8b , -C(0)0R 8b , -0C(0)R 8b , - 0C(0)0R 8b , -N(R 8b R 8c ), -N(R 8b )C(0)R 8c , -C(0)N(R 8b R 8c ), -N(R 8b )C(0)0R 8c , - 0C(0)N(R 8b R 8c ), -N(R 8b )C(0)0R 8c , - 0C(0)N(R 8b R 8c ), -N(R 8b )C(0)N(R 8c R 8d ), -C(0)
  • the present invention provides a compound of Formula (la), or a pharmaceutically acceptable salt or isomer thereof, wherein the compound has the following structure:
  • R 1 is hydrogen or Ci- 6 alkyl
  • R 3a is hydrogen, Ci- 6 alkyl, C 3-8 cycloalkyl, or C 4-14 alkyl-cycloalkyl
  • R 3c is hydrogen or Ci- 6 alkyl
  • R 4 , R 5 , and R 7 are each independently hydrogen, Ci- 6 alkyl, C2-6 alkenyl, C2-6 alkynyl, halogen, Ci- 6 haloalkyl, Ci- 6 alkylamine, Ci- 6 alkoxy, Ci-e haloalkoxy, -OR 8a , -NO2, -CN, -C(0)R 8b , -C(0)0R 8b , -0C(0)R 8b , -0C(0)0R 8b , -N(R 8b R 8c ), -N(R 8b )C(0)R 8c , -C(0)N(R 8b R 8c ), -N(R 8b )C(0)0R
  • R 8a , R 8b , R 8c , and R 8d are each independently hydrogen, Ci- 6 alkyl, or C 3-6 cycloalkyl; n is 1, 2, or 3; or salts and isomers thereof; and wherein when n is 1 and R 6 is Ci- 6 alkyl, halo, C(0)H, NH 2 , or Ci- 6 alkoxy, then at least one of R 4 , R 5 , and R 7 is not hydrogen; wherein when n is 1 and R 6 is methyl, halogen, or C(0)H, then at least one of R 4 , R 5 , or R 7 is not methyl or Cl; wherein when n is 2 and R 6 is F, Cl, methyl, or OMe, then R 3a is methyl; and wherein when n is 2 and R 6 is F, then at least one of R 4 , R 5 , and R 7 is not hydrogen.
  • the present invention provides a compound, or a
  • R 3a is methyl, and R 2 and R 3b are combined with the atoms to which they are attached to form a Ce-i heterocycloalkyl.
  • the present invention provides a compound, or a
  • the present invention provides a compound, or a pharmaceutically acceptable salt thereof, wherein the compound of Formula I or la has the following structure:
  • the present invention provides a compound, or a pharmaceutically acceptable salt thereof, wherein the compound of Formula I or la has the following structure:
  • R 5 and R 6 are each independently H, F, Cl, Br, I, -OH, -OMe, -OiPr, -Me, -CF3,- OCF3, -NMe2, -NHC(0)Me, or -N(Me)C(0)Me, wherein at least one of R 5 and R 6 is not H; alternatively, R 5 and R 6 are combined to form a 1,3-dioxole ring or 1,4-dioxane ring.
  • the present invention provides a compound, or a pharmaceutically acceptable salt thereof, wherein the compound of Formula I or la has the following structure:
  • R 5 and R 6 are each independently F, Cl, Br, I, -OH, -OMe, -OiPr, -Me, -CF3,-OCF3, -NMe2, -NHC(0)Me, or -N(Me)C(0)Me; alternatively, R 5 and R 6 are combined to form a 1,3-dioxole ring or 1,4-dioxane ring.
  • the present invention provides a compound, wherein the compound of Formula I or la has the following structure:
  • R 5 is H, F, Cl, Br, -OH or -OMe
  • R 6 is H, F, -OMe, -CF3 or -OCF3, wherein at least one of R 5 and R 6 is not H.
  • R 4 and R 6 are each independently H, F, Cl, Br, I, -OH, -OMe, -OiPr, -Me, -CF3,- OCF3, -NMe2, -NHC(0)Me, or -N(Me)C(0)Me, wherein at least one of R 4 and R 6 is not H.
  • the present invention provides a compound, or a
  • R 4 and R 6 are each independently F, Cl, Br, I, -OH, -OMe, -OiPr, -Me, -CF3,-OCF3, -NMe 2 , -NHC(0)Me, or -N(Me)C(0)Me.
  • the present invention provides a compound, wherein the compound is:
  • the present invention provides a compound, or a pharmaceutically acceptable salt thereof, wherein the compound is
  • the present invention provides a compound, or a pharmaceutically acceptable salt thereof, wherein the compound is
  • the present invention provides a compound, or a 5 pharmaceutically acceptable salt thereof, wherein the compound is
  • the present invention provides a compound, or a
  • the present invention provides a compound, or a
  • R 3a is hydrogen, and R 2 and R 3b are combined with the atoms to which they are attached to form a C heterocycloalkyl.
  • the present invention provides a compound, or a pharmaceutically acceptable salt thereof, wherein the compound of Formula I or la has the following structure:
  • the present invention provides a compound, or a pharmaceutically acceptable salt thereof, wherein the compound is
  • R 3c is hydrogen or Ci- 6 alkyl. In some embodiments, R 3c is hydrogen, methyl, ethyl, or propyl. In some embodiments R 3c is hydrogen.
  • R 1 is Ci- 6 alkyl. In some embodiments, R 1 is methyl, ethyl, or propyl. In some embodiments, the present invention provides a compound, or a pharmaceutically acceptable salt thereof, wherein the compound of Formula I or la has the following structure:
  • the present invention provides a compound, or a pharmaceutically acceptable salt thereof, wherein the compound is [0119] In some embodiments, the present invention provides a compound, or a
  • the present invention provides a compound, or a
  • the present invention provides a compound, or a
  • the compounds of the present invention can also be in the salt forms, such as acid or base salts of the compounds of the present invention.
  • salt forms such as acid or base salts of the compounds of the present invention.
  • pharmaceutically acceptable salts are mineral acid (hydrochloric acid, hydrobromic acid, phosphoric acid, and the like) salts, organic acid (fumaric acid, acetic acid, propionic acid, glutamic acid, citric acid and the like) salts, quaternary ammonium (methyl iodide, ethyl iodide, and the like) salts. It is understood that the pharmaceutically acceptable salts are non toxic. Additional information on suitable pharmaceutically acceptable salts can be found in Remington's Pharmaceutical Sciences, 17th ed., Mack Publishing Company, Easton, Pa., 1985, which is incorporated herein by reference.
  • the present invention also includes isotopically-labeled compounds of the present invention, wherein one or more atoms are replaced by one or more atoms having specific atomic mass or mass numbers.
  • isotopes that can be incorporated into compounds of the invention include, but are not limited to, isotopes of hydrogen, carbon, nitrogen, oxygen, fluorine, sulfur, and chlorine (such as 2 H, 3 H, 13 C, 14 C, 15 N, 18 0, 17 0, 18 F,
  • Isotopically-labeled compounds of the present invention are useful in assays of the tissue distribution of the compounds and their prodrugs and metabolites; preferred isotopes for such assays include 3 H and 14 C.
  • substitution with heavier isotopes, such as deuterium ( 2 H) can provide increased metabolic stability, which offers therapeutic advantages such as increased in vivo half-life or reduced dosage requirements.
  • Isotopically-labeled compounds of this invention can generally be prepared according to the methods known by one of skill in the art by substituting an isotopically- labeled reagent for a non-isotopically labeled reagent.
  • Compounds of the present invention can be isotopically labeled at positions adjacent to the basic amine, in aromatic rings, and the methyl groups of methoxy substituents.
  • the present invention includes all tautomers and stereoisomers of compounds of the present invention, either in admixture or in pure or substantially pure form.
  • the compounds of the present invention can have asymmetric centers at the carbon atoms, and therefore the compounds of the present invention can exist in diastereomeric or enantiomeric forms or mixtures thereof. All conformational isomers (e.g., cis and trans isomers) and all optical isomers (e.g., enantiomers and diastereomers), racemic, diastereomeric and other mixtures of such isomers, as well as solvates, hydrates, isomorphs, polymorphs and tautomers are within the scope of the present invention.
  • Compounds according to the present invention can be prepared using diastereomers, enantiomers or racemic mixtures as starting materials.
  • diastereomer and enantiomer products can be separated by chromatography, fractional crystallization or other methods known to those of skill in the art.
  • the present invention provides a pharmaceutical composition
  • a pharmaceutical composition comprising a compound of the present invention, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient.
  • compositions of the present invention can be prepared in a wide variety of oral, parenteral and topical dosage forms.
  • Oral preparations include tablets, pills, powder, capsules, liquids, lozenges, cachets, gels, syrups, slurries, suspensions, etc., suitable for ingestion by the patient.
  • the compositions of the present invention can also be administered by injection, that is, intravenously, intramuscularly, intracutaneously, subcutaneously, intraduodenally, or intraperitoneally.
  • the compositions described herein can be administered by inhalation, for example, intranasally. Additionally, the compositions of the present invention can be administered transdermally.
  • compositions of this invention can also be administered by intraocular, intravaginal, and intrarectal routes including suppositories, insufflation, powders and aerosol formulations (for examples of steroid inhalants, see Rohatagi, J. Clin. Pharmacol. 35: 1187-1193, 1995; Tjwa, Ann. Allergy Asthma Immunol. 75: 107-111, 1995).
  • the present invention also provides pharmaceutical compositions including a pharmaceutically acceptable carrier or excipient and the compounds of the present invention.
  • pharmaceutically acceptable carriers can be either solid or liquid.
  • Solid form preparations include powders, tablets, pills, capsules, cachets, suppositories, and dispersible granules.
  • a solid carrier can be one or more substances, which may also act as diluents, flavoring agents, binders, preservatives, tablet disintegrating agents, or an encapsulating material. Details on techniques for formulation and administration are well described in the scientific and patent literature, see, e.g., the latest edition of Remington's Pharmaceutical Sciences, Maack Publishing Co, Easton PA ("Remington's").
  • the carrier is a finely divided solid, which is in a mixture with the finely divided active component.
  • the active component is mixed with the carrier having the necessary binding properties in suitable proportions and compacted in the shape and size desired.
  • the powders and tablets preferably contain from 5% to 70% or 10% to 70% of the compounds of the present invention.
  • Suitable solid excipients include, but are not limited to, magnesium carbonate; magnesium stearate; talc; pectin; dextrin; starch; tragacanth; a low melting wax; cocoa butter; carbohydrates; sugars including, but not limited to, lactose, sucrose, mannitol, or sorbitol, starch from com, wheat, rice, potato, or other plants; cellulose such as methyl cellulose, hydroxypropylmethyl-cellulose, or sodium carboxymethylcellulose; and gums including arabic and tragacanth; as well as proteins including, but not limited to, gelatin and collagen.
  • disintegrating or solubilizing agents may be added, such as the cross-linked polyvinyl pyrrolidone, agar, alginic acid, or a salt thereof, such as sodium alginate.
  • a low melting wax such as a mixture of fatty acid glycerides or cocoa butter
  • the compounds of the present invention are dispersed homogeneously therein, as by stirring.
  • the molten homogeneous mixture is then poured into convenient sized molds, allowed to cool, and thereby to solidify.
  • Liquid form preparations include solutions, suspensions, and emulsions, for example, water or water/propylene glycol solutions.
  • liquid preparations can be formulated in solution in aqueous polyethylene glycol solution.
  • Aqueous solutions suitable for oral use can be prepared by dissolving the compounds of the present invention in water and adding suitable colorants, flavors, stabilizers, and thickening agents as desired.
  • Aqueous suspensions suitable for oral use can be made by dispersing the finely divided active component in water with viscous material, such as natural or synthetic gums, resins, methylcellulose, sodium carboxymethylcellulose, hydroxypropylmethylcellulose, sodium alginate, polyvinylpyrrolidone, gum tragacanth and gum acacia, and dispersing or wetting agents such as a naturally occurring phosphatide (e.g., lecithin), a condensation product of an alkylene oxide with a fatty acid (e.g., polyoxyethylene stearate), a condensation product of ethylene oxide with a long chain aliphatic alcohol (e.g., heptadecaethylene oxycetanol), a condensation product of ethylene oxide with a partial ester derived from a fatty acid and a
  • the aqueous suspension can also contain one or more preservatives such as ethyl or n-propyl p-hydroxybenzoate, one or more coloring agents, one or more flavoring agents and one or more sweetening agents, such as sucrose, aspartame or saccharin.
  • preservatives such as ethyl or n-propyl p-hydroxybenzoate
  • coloring agents such as a coloring agent
  • flavoring agents such as aqueous suspension
  • sweetening agents such as sucrose, aspartame or saccharin.
  • Formulations can be adjusted for osmolarity.
  • solid form preparations which are intended to be converted, shortly before use, to liquid form preparations for oral administration.
  • liquid forms include solutions, suspensions, and emulsions.
  • These preparations may contain, in addition to the active component, colorants, flavors, stabilizers, buffers, artificial and natural sweeteners, dispersants, thickeners, solubilizing agents, and the like.
  • Oil suspensions can be formulated by suspending the compound of the present invention in a vegetable oil, such as arachis oil, olive oil, sesame oil or coconut oil, or in a mineral oil such as liquid paraffin; or a mixture of these.
  • the oil suspensions can contain a thickening agent, such as beeswax, hard paraffin or cetyl alcohol.
  • Sweetening agents can be added to provide a palatable oral preparation, such as glycerol, sorbitol or sucrose.
  • These formulations can be preserved by the addition of an antioxidant such as ascorbic acid.
  • an injectable oil vehicle see Minto, J. Pharmacol. Exp. Ther. 281 :93-102, 1997.
  • the pharmaceutical formulations of the invention can also be in the form of oil-in-water emulsions.
  • the oily phase can be a vegetable oil or a mineral oil, described above, or a mixture of these.
  • Suitable emulsifying agents include naturally-occurring gums, such as gum acacia and gum tragacanth, naturally occurring phosphatides, such as soybean lecithin, esters or partial esters derived from fatty acids and hexitol anhydrides, such as sorbitan mono- oleate, and condensation products of these partial esters with ethylene oxide, such as polyoxyethylene sorbitan mono-oleate.
  • the emulsion can also contain sweetening agents and flavoring agents, as in the formulation of syrups and elixirs. Such formulations can also contain a demulcent, a preservative, or a coloring agent.
  • compositions of the present invention can also be delivered as microspheres for slow release in the body.
  • microspheres can be formulated for administration via intradermal injection of drug- containing microspheres, which slowly release
  • microspheres for oral administration see, e.g., Eyles, J. Pharm. Pharmacol. 49:669-674, 1997. Both transdermal and intradermal routes afford constant delivery for weeks or months.
  • the pharmaceutical compositions of the present invention can be formulated for parenteral administration, such as intravenous (IV) administration or administration into a body cavity or lumen of an organ.
  • the formulations for administration will commonly comprise a solution of the compositions of the present invention dissolved in a pharmaceutically acceptable carrier.
  • acceptable vehicles and solvents that can be employed are water and Ringer's solution, an isotonic sodium chloride.
  • sterile fixed oils can conventionally be employed as a solvent or suspending medium.
  • any bland fixed oil can be employed including synthetic mono- or diglycerides.
  • fatty acids such as oleic acid can likewise be used in the preparation of injectables. These solutions are sterile and generally free of undesirable matter.
  • formulations may be sterilized by conventional, well known sterilization techniques.
  • the formulations may contain pharmaceutically acceptable auxiliary substances as required to approximate physiological conditions such as pFI adjusting and buffering agents, toxicity adjusting agents, e.g., sodium acetate, sodium chloride, potassium chloride, calcium chloride, sodium lactate and the like.
  • concentration of the compositions of the present invention in these formulations can vary widely, and will be selected primarily based on fluid volumes, viscosities, body weight, and the like, in accordance with the particular mode of administration selected and the patient's needs.
  • the formulation can be a sterile injectable preparation, such as a sterile injectable aqueous or oleaginous suspension.
  • This suspension can be formulated according to the known art using those suitable dispersing or wetting agents and suspending agents.
  • the sterile injectable preparation can also be a sterile injectable solution or suspension in a nontoxic parenterally-acceptable diluent or solvent, such as a solution of 1,3-butanediol.
  • the formulations of the compositions of the present invention can be delivered by the use of liposomes which fuse with the cellular membrane or are endocytosed, i.e., by employing ligands attached to the liposome, or attached directly to the oligonucleotide, that bind to surface membrane protein receptors of the cell resulting in endocytosis.
  • liposomes particularly where the liposome surface carries ligands specific for target cells, or are otherwise preferentially directed to a specific organ, one can focus the delivery of the compositions of the present invention into the target cells in vivo. (See, e.g., Al-Muhammed, J. Microencapsul. 13:293-306, 1996; Chonn, Curr. Opin.
  • compositions of the present invention can be delivered by any suitable means, including oral, parenteral and topical methods.
  • Transdermal administration methods by a topical route, can be formulated as applicator sticks, solutions, suspensions, emulsions, gels, creams, ointments, pastes, jellies, paints, powders, and aerosols.
  • the pharmaceutical preparation is preferably in unit dosage form.
  • the preparation is subdivided into unit doses containing appropriate quantities of the compounds of the present invention.
  • the unit dosage form can be a packaged preparation, the package containing discrete quantities of preparation, such as packeted tablets, capsules, and powders in vials or ampoules.
  • the unit dosage form can be a capsule, tablet, cachet, or lozenge itself, or it can be the appropriate number of any of these in packaged form.
  • the compound of the present invention can be present in any suitable amount, and can depend on various factors including, but not limited to, weight and age of the subject, state of the disease, etc.
  • Suitable dosage ranges for the compound of the present invention include from about 0.1 mg to about 10,000 mg, or about 1 mg to about 1000 mg, or about 10 mg to about 750 mg, or about 25 mg to about 500 mg, or about 50 mg to about 250 mg.
  • Suitable dosages for the compound of the present invention include about 1 mg, 5, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500, 600, 700, 800, 900 or 1000 mg.
  • the compounds of the present invention can be administered at any suitable frequency, interval and duration.
  • the compound of the present invention can be administered once an hour, or two, three or more times an hour, once a day, or two, three, or more times per day, or once every 2, 3, 4, 5, 6, or 7 days, so as to provide the preferred dosage level.
  • representative intervals include 5, 10, 15, 20, 30, 45 and 60 minutes, as well as 1, 2, 4,
  • the compound of the present invention can be administered once, twice, or three or more times, for an hour, for 1 to 6 hours, for 1 to 12 hours, for 1 to 24 hours, for 6 to 12 hours, for 12 to 24 hours, for a single day, for 1 to 7 days, for a single week, for 1 to 4 weeks, for a month, for 1 to 12 months, for a year or more, or even indefinitely.
  • composition can also contain other compatible therapeutic agents.
  • the compounds described herein can be used in combination with one another, with other active agents known to be useful in modulating a glucocorticoid receptor, or with adjunctive agents that may not be effective alone, but may contribute to the efficacy of the active agent.
  • the compounds of the present invention can be co-administered with another active agent.
  • Co-administration includes administering the compound of the present invention and active agent within 0.5, 1, 2, 4, 6, 8, 10, 12, 16, 20, or 24 hours of each other.
  • Co administration also includes administering the compound of the present invention and active agent simultaneously, approximately simultaneously (e.g., within about 1, 5, 10, 15, 20, or 30 minutes of each other), or sequentially in any order.
  • the compound of the present invention and the active agent can each be administered once a day, or two, three, or more times per day so as to provide the preferred dosage level per day.
  • co-administration can be accomplished by co-formulation, i.e., preparing a single pharmaceutical composition including both the compound of the present invention and the active agent.
  • the compound of the present invention and the active agent can be formulated separately.
  • the compound of the present invention and the active agent can be present in the compositions of the present invention in any suitable weight ratio, such as from about 1 :100 to about 100: 1 (w/w), or about 1 :50 to about 50: 1 , or about 1 :25 to about 25: 1 , or about 1 :10 to about 10:1, or about 1 :5 to about 5:1 (w/w).
  • the compound of the present invention and the other active agent can be present in any suitable weight ratio, such as about 1: 100 (w/w), 1 :50, 1 :25, 1 : 10, 1:5, 1 :4, 1 :3, 1:2, 1 : 1, 2: 1, 3:1, 4: 1, 5: 1, 10:1, 25: 1, 50: 1 or 100: 1 (w/w).
  • suitable weight ratio such as about 1: 100 (w/w), 1 :50, 1 :25, 1 : 10, 1:5, 1 :4, 1 :3, 1:2, 1 : 1, 2: 1, 3:1, 4: 1, 5: 1, 10:1, 25: 1, 50: 1 or 100: 1 (w/w).
  • Other dosages and dosage ratios of the compound of the present invention and the active agent are suitable in the compositions and methods of the present invention.
  • the compounds of the present invention can be used for increasing neuronal plasticity.
  • the compounds of the present invention can also be used to treat any brain disease.
  • the compounds of the present invention can also be used for increasing at least one of translation, transcription or secretion of neurotrophic factors.
  • a compound of the present invention is used to treat neurological diseases.
  • the compounds have, for example, anti- addictive properties, antidepressant properties, anxiolytic properties, or a combination thereof.
  • the neurological disease is a neuropsychiatric disease.
  • the neuropsychiatric disease is a mood or anxiety disorder.
  • the neurological disease is a migraine, headaches (e.g., cluster headache), post- traumatic stress disorder (PTSD), anxiety, depression, neurodegenerative disorder,
  • Alzheimer’s disease, Parkinson’s disease psychological disorder, treatment resistant depression, suicidal ideation, major depressive disorder, bipolar disorder, schizophrenia, stroke, traumatic brain injury, and addiction (e.g., substance use disorder).
  • the neurological disease is a migraine or cluster headache.
  • the neurological disease is a neurodegenerative disorder, Alzheimer’s disease, or Parkinson’s disease.
  • the neurological disease is a psychological disorder, treatment resistant depression, suicidal ideation, major depressive disorder, bipolar disorder, schizophrenia, post-traumatic stress disorder (PTSD), addiction (e.g., substance use disorder), depression, or anxiety.
  • the neuropsychiatric disease is a psychological disorder, treatment resistant depression, suicidal ideation, major depressive disorder, bipolar disorder, schizophrenia, post-traumatic stress disorder (PTSD), addiction (e.g., substance use disorder), depression, or anxiety.
  • the neuropsychiatric disease is a psychological disorder, treatment resistant depression, suicidal ideation, major depressive disorder, bipolar disorder, schizophrenia, post-traumatic stress disorder (PTSD), addiction (e.g., substance use disorder), depression, or anxiety.
  • the neuropsychiatric disease is a psychological disorder, treatment resistant depression, suicidal ideation, major depressive disorder, bipolar disorder, schizophrenia, post-traumatic stress disorder (PTSD), addiction (e.g., substance use disorder), depression, or anxiety.
  • the neuropsychiatric disease is a psychological disorder, treatment resistant depression, suicidal ideation, major depressive disorder, bipolar disorder, schizophrenia, post-traumatic stress disorder (PTSD), addiction (e.g., substance use disorder), depression, or anxiety.
  • neuropsychiatric disease or neurological disease is post-traumatic stress disorder (PTSD), addiction (e.g., substance use disorder), schizophrenia, depression, or anxiety.
  • the neuropsychiatric disease or neurological disease is addiction (e.g., substance use disorder).
  • the neuropsychiatric disease or neurological disease is depression.
  • the neuropsychiatric disease or neurological disease is anxiety.
  • the neuropsychiatric disease or neurological disease is post-traumatic stress disorder (PTSD).
  • the neurological disease is stroke or traumatic brain injury.
  • the neuropsychiatric disease or neurological disease is schizophrenia.
  • a compound of the present invention is used for increasing neuronal plasticity.
  • the compounds described herein are used for treating a brain disorder.
  • the compounds described herein are used for increasing at least one of translation, transcription, or secretion of neurotrophic factors.
  • the compounds of the present invention have activity as 5- HT2 A modulators. In some embodiments, the compounds of the present invention have activity as 5-HT 2A modulators. In some embodiments, the compounds of the present invention elicit a biological response by activating the 5-HT 2A receptor (e.g., allosteric modulation or modulation of a biological target that activates the 5-HT 2A receptor). 5-HT 2A agonism has been correlated with the promotion of neural plasticity (Ly et al., 2018).
  • Hallucinogens e.g., LSD and 5-MeO-DMT
  • LSD and 5-MeO-DMT activate a 5HT2 A sensor assay in agonist mode, but their non-hallucinogenic congeners (lisuride (LIS) and 6-MeO-DMT) do not (FIG. 15).
  • compounds such as, for example, 5-MeO-DMT, LSD, DMT, DOI, which are hallucinogenic in animals (e.g., humans), activate the 5HT2 A sensor assay in agonist mode
  • compounds such as, for example, 6-MeO-DMT, LIS, 6-F-DET, L-MDMA, R- MDMA, Ketanserin, BOL148, which are non-hallucinogenic in animals (e.g., humans), do not activate the 5HT2 A sensor assay in agonist mode (FIG. 16, at 10 mM of compound).
  • hallucinogenic potential of a compound of the present invention is determined in vitro.
  • hallucinogenic potential of a compound of the present invention is determined using a 5HT 2A sensor assay.
  • the 5HT 2A sensor assay is in an agonist mode or an antagonist mode.
  • the 5HT 2A sensor assay is in an agonist mode.
  • a compound of the present invention that does not activate the sensor in agonist mode has non-hallucinogenic potential.
  • a compound of the present invention that does not activate the sensor in agonist mode is a non-hallucinogenic compound.
  • non-hallucinogenic compounds e.g., lisuride and 6-MeO-DMT
  • compounds such as, for example, 6-F-DET, Ketanserin, BOL148, which are non-hallucinogenic in animals (e.g., humans)
  • 5-HT binding to 5HT2 A in the antagonist mode sensor assay FIG. 18, at 10 mM of compound.
  • the 5HT2 A sensor assay is in an antagonist mode.
  • the results for the agonist mode sensor assay suggests a compound of the present invention is a non-hallucinogenic ligand of the 5-HT2 A receptor.
  • the results for the antagonist mode sensor assay suggests a compound of the present invention is a non-hallucinogenic ligand of the 5-HT2 A receptor.
  • the results for the agonist mode and antagonist mode sensor assay together suggest a compound of the present invention is a non-hallucinogenic ligand of the 5-HT2 A receptor.
  • the compounds described herein are selective 5-HT2 A modulators. In some embodiments, the compounds described herein are 5-HT 2A modulators and promote neural plasticity (e.g., cortical structural plasticity). In some embodiments, the compounds described herein are selective 5-HT 2A modulators and promote neural plasticity (e.g., cortical structural plasticity). In some embodiments, promotion of neural plasticity includes, for example, increased dendritic spine growth, increased synthesis of synaptic proteins, strengthened synaptic responses, increased dendritic arbor complexity, increased dendritic branch content, increased spinogenesis, increased neuritogenesis, or any combination thereof. In some embodiments, increased neural plasticity includes, for example, increased cortical structural plasticity in the anterior parts of the brain.
  • the 5-HT2 A modulators are non- hallucinogenic.
  • non-hallucinogenic 5-HT 2A modulators e.g., 5-HT 2A agonists
  • the hallucinogenic potential of the compounds described herein is assessed in vitro.
  • the hallucinogenic potential assessed in vitro of the compounds described herein is compared to the hallucinogenic potential assessed in vitro of hallucinogenic homologs.
  • the compounds described herein elicit less hallucinogenic potential in vitro than the hallucinogenic homologs.
  • non-hallucinogenic 5-HT 2A modulators e.g., 5-HT 2A agonists
  • the neurological diseases comprise decreased neural plasticity, decreased cortical structural plasticity, decreased 5-HT 2A receptor content, decreased dendritic arbor complexity, loss of dendritic spines, decreased dendritic branch content, decreased spinogenesis, decreased neuritogenesis, retraction of neurites, or any combination thereof.
  • non-hallucinogenic 5-HT 2A modulators e.g., 5-HT 2A agonists
  • non- hallucinogenic 5-HT 2A modulators e.g., 5-HT 2A agonists
  • non-hallucinogenic 5-HT2 A modulators are used for increasing at least one of translation, transcription, or secretion of neurotrophic factors.
  • Neuronal plasticity refers to the ability of the brain to change structure and/or function throughout a subject’s life. New neurons can be produced and integrated into the central nervous system throughout the subject’s life. Increasing neuronal plasticity includes, but is not limited to, promoting neuronal growth, promoting neuritogenesis, promoting synaptogenesis, promoting dendritogenesis, increasing dendritic arbor complexity, increasing dendritic spine density, and increasing excitatory synapsis in the brain. In some
  • increasing neuronal plasticity comprises promoting neuronal growth, promoting neuritogenesis, promoting synaptogenesis, promoting dendritogenesis, increasing dendritic arbor complexity, and increasing dendritic spine density.
  • increasing neuronal plasticity can treat neurodegenerative disorder, Alzheimer’s, Parkinson’s disease, psychological disorder, depression, addiction, anxiety, post-traumatic stress disorder, treatment resistant depression, suicidal ideation, major depressive disorder, bipolar disorder, schizophrenia, stroke, traumatic brain injury, or substance use disorder.
  • the present invention provides methods for increasing neuronal plasticity, comprising contacting a neuronal cell with any of the compounds of the present invention. In some embodiments, increasing neuronal plasticity improves a brain disorder described herein.
  • a compound of the present invention is used to increase neuronal plasticity.
  • the compounds used to increase neuronal plasticity have, for example, anti- addictive properties, antidepressant properties, anxiolytic properties, or a combination thereof.
  • decreased neuronal plasticity is associated with a neuropsychiatric disease.
  • the neuropsychiatric disease is a mood or anxiety disorder.
  • the neuropsychiatric disease includes, for example, migraine, cluster headache, post-traumatic stress disorder (PTSD), schizophrenia, anxiety, depression, and addiction (e.g., substance abuse disorder).
  • brain disorders include, for example, migraines, addiction (e.g., substance use disorder), depression, and anxiety.
  • the experiment or assay to determine increased neuronal plasticity of any compound of the present invention is a phenotypic assay, a dendritogenesis assay, a spinogenesis assay, a synaptogenesis assay, a Sholl analysis, a concentration- response experiment, a 5-HT 2A agonist assay, a 5-HT 2A antagonist assay, a 5-HT 2A binding assay, or a 5-HT2 A blocking experiment (e.g., ketanserin blocking experiments).
  • the experiment or assay to determine the hallucinogenic potential of any compound of Formula I or Formula (la) is a mouse head-twitch response (HTR) assay.
  • the present invention provides a method for increasing neuronal plasticity, comprising contacting a neuronal cell with a compound of Formula I:
  • R 1 is hydrogen or Ci- 6 alkyl
  • R 3a and R 3b are each independently hydrogen, Ci- 6 alkyl, Cvs cycloalkyl, or C 4-14 alkyl-cycloalkyl
  • R 3c is hydrogen or Ci- 6 alkyl
  • R 2 and R 3b are combined with the atoms to which they are attached to form a C5-8 heterocycloalkyl substituted with 1 to 3 R 9 groups which are each independently hydrogen, Ci-6 alkyl, C3-8 cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10
  • R 8a , R 8b , R 8c and R 8d are each independently H, Ci- 6 alkyl.
  • the present invention provides a method for increasing neuronal plasticity, comprising contacting a neuronal cell with a compound of Formula I, wherein: R 1 is hydrogen or Ci- 6 alkyl; R 3a and R 3b are each independently hydrogen, Ci- 6 alkyl, Civs cycloalkyl, or C 4-14 alkyl-cycloalkyl; R 3c is hydrogen or Ci- 6 alkyl; alternatively,
  • R 8b , R 8c and R 8d are each independently H or Ci- 6 alkyl; or pharmaceutically acceptable salts and isomers thereof, in an amount sufficient to increase neuronal plasticity of the neuronal cell.
  • the present invention provides a method of treating a disease, including administering to a subject in need thereof, a therapeutically effective amount of a compound of the present invention. In some embodiments, the present invention provides a method of treating a brain disorder, including administering to a subject in need thereof, a therapeutically effective amount of a compound of the present invention. In some embodiments, the present invention provides a method of treating a brain disorder with combination therapy, including administering to a subject in need thereof, a therapeutically effective amount of a compound of the present invention and at least one additional therapeutic agent.
  • 5-HT2 A modulators e.g., 5-HT2 A agonists
  • the brain disorders comprise decreased neural plasticity, decreased cortical structural plasticity, decreased 5-HT 2A receptor content, decreased dendritic arbor complexity, loss of dendritic spines, decreased dendritic branch content, decreased spinogenesis, decreased neuritogenesis, retraction of neurites, or any combination thereof.
  • a compound of the present invention is used to treat brain disorders.
  • the compounds have, for example, anti- addictive properties, antidepressant properties, anxiolytic properties, or a combination thereof.
  • the brain disorder is a neuropsychiatric disease.
  • the neuropsychiatric disease is a mood or anxiety disorder.
  • brain disorders include, for example, migraine, cluster headache, post-traumatic stress disorder (PTSD), anxiety, depression, schizophrenia, and addiction (e.g., substance abuse disorder).
  • brain disorders include, for example, migraines, addiction (e.g., substance use disorder), depression, and anxiety.
  • the present invention provides a method of treating a brain disorder, comprising administering to a subject in need thereof a therapeutically effective amount of a compound of Formula I:
  • R 1 is hydrogen or Ci- 6 alkyl
  • R 3a and R 3b are each independently hydrogen, Ci- 6 alkyl, CYx cycloalkyl, or C 4-14 alkyl-cycloalkyl
  • R 3c is hydrogen or Ci- 6 alkyl
  • the present invention provides a method of treating a brain disorder, comprising administering to a subject in need thereof a therapeutically effective amount of a compound of Formula I, wherein: R 1 is hydrogen or Ci- 6 alkyl; R 3a and R 3b are each independently hydrogen, Ci- 6 alkyl, CYx cycloalkyl, or C 4-14 alkyl-cycloalkyl; R 3c is hydrogen or Ci- 6 alkyl; alternatively, R 2 and R 3b are combined with the atoms to which they are attached to form a CYx heterocycloalkyl substituted with 1 to 3 R 9 groups which are each independently hydrogen, Ci- 6 alkyl, CYx cycloalkyl, C 3-14 alkyl-cycloalkyl, C 4-10
  • R 4 , R 5 , R 6 and R 7 are each independently H or Ci- 6 alkyl; or pharmaceutically acceptable salts and isomers thereof, thereby treating the brain disorder
  • the brain disorder is a neurodegenerative disorder
  • Alzheimer’s, Parkinson’s disease psychological disorder, depression, addiction, anxiety, post-traumatic stress disorder, treatment resistant depression, suicidal ideation, major depressive disorder, bipolar disorder, schizophrenia, stroke, traumatic brain injury, or substance use disorder.
  • the brain disorder is a neurodegenerative disorder
  • the brain disorder is a psychological disorder, depression, addiction, anxiety, or a post-traumatic stress disorder.
  • the brain disorder is depression.
  • the brain disorder is addiction.
  • the brain disorder is treatment resistant depression, suicidal ideation, major depressive disorder, bipolar disorder, schizophrenia, stroke, traumatic brain injury or substance use disorder.
  • the brain disorder is treatment resistant depression, suicidal ideation, major depressive disorder, bipolar disorder, schizophrenia, or substance use disorder.
  • the brain disorder is treatment resistant depression, suicidal ideation, major depressive disorder, bipolar disorder, or substance use disorder.
  • the brain disorder is stroke or traumatic brain injury.
  • the brain disorder is schizophrenia.
  • the brain disorder is alcohol use disorder.
  • the method further comprises administering one or more additional therapeutic agent that is lithium, Olanzapine (Zyprexa), Quetiapine (Seroquel), Risperidone (Risperdal), Ariprazole (Abilify), Ziprasidone (Geodon), Clozapine (Clozaril), divalproex sodium (Depakote), lamotrigine (Lamictal), valproic acid (Depakene), carbamazepine (Equetro), topiramate (Topamax), levomilnacipran (Fetzima), duloxetine (Cymbalta, Yentreve), venlafaxine (Effexor), citalopram (Celexa), fluvoxamine (Luvox), escitalopram (Lexapro), fluoxetine (Prozac), paroxetine (Paxil), sertraline (Zoloft), clomipramine (Anafranil),
  • the compounds of the present invention are used in combination with the standard of care therapy for a neurological disease described herein.
  • the standard of care therapies may include, for example, lithium, olanzapine, quetiapine, risperidone, ariprazole, ziprasidone, clozapine, divalproex sodium, lamotrigine, valproic acid, carbamazepine, topiramate, levomilnacipran, duloxetine, venlafaxine, citalopram, fluvoxamine, escitalopram, fluoxetine, paroxetine, sertraline, clomipramine, amitriptyline, desipramine, imipramine, nortriptyline, phenelzine, tranylcypromine, diazepam, alprazolam, clonazepam, or any combination thereof.
  • Non limiting examples of standard of care therapy for depression are sertraline, fluoxetine, escitalopram, venlafaxine, or aripiprazole.
  • Non-limiting examples of standard of care therapy for depression are citralopram, escitalopram, fluoxetine, paroxetine, diazepam, or sertraline.
  • Neurotrophic factors refers to a family of soluble peptides or proteins which support the survival, growth, and differentiation of developing and mature neurons. Increasing at least one of translation, transcription, or secretion of neurotrophic factors can be useful for, but not limited to, increasing neuronal plasticity, promoting neuronal growth, promoting
  • neuritogenesis promoting synaptogenesis, promoting dendritogenesis, increasing dendritic arbor complexity, increasing dendritic spine density, and increasing excitatory synapsis in the brain.
  • increasing at least one of translation, transcription, or secretion of neurotrophic factors can increasing neuronal plasticity.
  • increasing at least one of translation, transcription, or secretion of neurotrophic factors can promoting neuronal growth, promoting neuritogenesis, promoting synaptogenesis, promoting dendritogenesis, increasing dendritic arbor complexity, and/or increasing dendritic spine density.
  • 5-HT 2A modulators e.g., 5-HT 2A agonists
  • 5-HT 2A agonists are used to increase at least one of translation, transcription, or secretion of neurotrophic factors.
  • a compound of Formula I or Formula (la) described herein is used increase at least one of translation, transcription, or secretion of neurotrophic factors.
  • increasing at least one of translation, transcription or secretion of neurotrophic factors treats a migraine, headaches (e.g., cluster headache), post-traumatic stress disorder (PTSD), anxiety, depression, neurodegenerative disorder, Alzheimer’s disease, Parkinson’s disease, psychological disorder, treatment resistant depression, suicidal ideation, major depressive disorder, bipolar disorder, schizophrenia, stroke, traumatic brain injury, and addiction (e.g., substance use disorder).
  • headaches e.g., cluster headache
  • PTSD post-traumatic stress disorder
  • anxiety depression
  • neurodegenerative disorder e.g., Alzheimer’s disease, Parkinson’s disease
  • psychological disorder e.g., treatment resistant depression
  • suicidal ideation e.g., major depressive disorder
  • bipolar disorder e.g., schizophrenia
  • stroke traumatic brain injury
  • addiction e.g., substance use disorder
  • the experiment or assay used to determine increase translation of neurotrophic factors includes ELISA, western blot, immunofluorescence assays, proteomic experiments, and mass spectrometry.
  • the experiment or assay used to determine increase transcription of neurotrophic factors includes gene expression assays, PCR, and microarrays.
  • the experiment or assay used to determine increase secretion of neurotrophic factors includes ELISA, western blot, immunofluorescence assays, proteomic experiments, and mass spectrometry.
  • the present invention provides a method for increasing at least one of translation, transcription or secretion of neurotrophic factors, comprising contacting a neuronal cell with a compound of Formula I:
  • R 1 is hydrogen or Ci-6 alkyl
  • R 3a and R 3b are each independently hydrogen, Ci- 6 alkyl, Cvx cycloalkyl, or C 4-14 alkyl-cycloalkyl
  • R 3c is hydrogen or Ci-6 alkyl
  • R 2 and R 3b are combined with the atoms to which they are attached to form a C 5-8 heterocycloalkyl substituted with 1 to 3 R 9 groups which are each independently hydrogen, Ci- 6 alkyl, Cvx cycloalkyl, C 3-14 alkyl-cycloalkyl, C 4-10
  • the present invention provides a method for increasing at least one of translation, transcription or secretion of neurotrophic factors, comprising contacting a neuronal cell with a compound of Formula I, wherein: R 1 is hydrogen or Ci- 6 alkyl; R 3a and R 3b are each independently hydrogen, Ci- 6 alkyl, C vx cycloalkyl, or C 4-14 alkyl- cycloalkyl; R 3c is hydrogen or Ci- 6 alkyl; alternatively, R 2 and R 3b are combined with the atoms to which they are attached to form a C 5-8 heterocycloalkyl substituted with 1 to 3 R 9 groups which are each independently hydrogen, Ci- 6 alkyl, CYx cycloalkyl, C 3-14 alkyl- cycloalkyl, C 4-10 heterocycloalkyl, C 4-16 alkyl-heterocycloalkyl, Ce-n aryl, C 7-18 alkyl-aryl, C 5- 10 heteroaryl
  • R 4 , R 5 , R 6 and R 7 are each independently hydrogen, Ci-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, halogen, Ci-6 haloalkyl, Ci-6 alkylamine, Ci-6 alkoxy, Ci-6 haloalkoxy, -OR 8a , -NO2, -CN, -
  • R 8b R 8c C(0)C(0)N (R 8b R 8c ), -S(0 2 )R 8b , -S(0) 2 N(R 8b R 8c ), C3-8 cycloalkyl, C3-14 alkyl-cycloalkyl, C - 10 heterocycloalkyl, C 4-16 alkyl-heterocycloalkyl, Ce-n aryl, C 7-18 alkyl-aryl, C 5-10 heteroaryl, or C4-16 alkyl-heteroaryl, wherein at least one of R 4 , R 5 , R 6 and R 7 is not H; R 8b , R 8c and R 8d are each independently H or Ci- 6 alkyl; or pharmaceutically acceptable salts and isomers thereof, in an amount sufficient to increase neuronal plasticity of the neuronal cell.
  • TLC Thin layer chromatography
  • Nuclear magnetic resonance (NMR) spectra were acquired on either a Bruker 400 operating at 400 and 100 MHz or a Varian-600 operating at 600 and 150 MHz for 1 1 1 and 13 C, respectively, and are referenced internally according to residual solvent signals.
  • Data for 1H NMR are recorded as follows: chemical shift (d, ppm), multiplicity (s, singlet; br s, broad singlet; d, doublet; t, triplet; q, quartet; quint, quintet; sext, sextet; m, multiplet), integration, and coupling constant (Hz).
  • Data for 13 C NMR are reported in terms of chemical shift (d, ppm).
  • Infrared spectra were recorded using a Thermo Scientific Nicolet iS lO spectrometer with Smart iTX Accessory (diamond ATR) and are reported in frequency of absorption. Low-resolution mass spectra were obtained using a Waters Acuity Arc LC-MS.
  • FIG. 1A The pharmacophore of ibogaine is depicted in FIG. 1A.
  • Embryonic rat cortical neurons were treated for a short period of time (1 h) followed by a suitable growth period (71 h) to measure the psychoplastogenic effects of the compounds described herein. Using this short treatment protocol (Dunlap, et al.
  • Ibogaine inhibits hERG channels with an IC50 of approximately 1 mM (FIG. 2D).
  • ibogaine As ibogaine is known to cause seizures at very high doses, seizurogenic potential using larval zebrafish expressing GCaMP5 was assessed. Neither ibogaine nor TBG produced excessive neural activity as was observed following treatment with the known seizure-inducing compound pentylenetetrazole (PTZ) (FIG. 10).
  • PTZ pentylenetetrazole
  • IBG and TBG demonstrated potent agonist activity at human (FIG. 2K) and mouse 5-HT2A receptors (FIG. 12).
  • Many 5-HT2A agonists such as 5-MeO-DMT, are also agonists of 5-HT2B receptors, which can lead to cardiac valvulopathy.
  • IBG and TBG act as antagonists at 5-HT2B receptors (FIG. 2K).
  • 5-MeO-DMT 5-HT2A receptors
  • both compounds displayed distinct profiles as compared to 5-MeO-DMT (FIG. 12, FIG. 13, and FIG. 14), including weaker 5-HT1A and 5-HT2C agonist activity.
  • IBG and TBG exhibit more selective, and potentially safer profiles than the less
  • the oily residue was dissolved in DCM ( ⁇ 25mL) and basified with 1M aqueous NaOH ( ⁇ 20mL). The aqueous layer was extracted with DCM (3 x 20 mL). The combined organic extracts were dried over Na 2 S0 4 and concentrated under reduced pressure to yield a mixture of the 6- and 4-substituted indoles.
  • the 6-substituted indole was purified by chromatography on silica gel (10: 1 DCM:MeOH with 0.5% NH 4 OH).
  • Fumaric acid (77 mg, 0.66 mmol, 0.8 equiv) was added to a sealed tube containing acetone (8 mL). The solution was carefully heated until all of the fumaric acid dissolved. After cooling the solution to room temperature, a solution of 18 free base (193 mg, 0.84 mmol, 1.0 equiv) in acetone (1 mL) was added, and the mixture was cooled in the freezer overnight. The solid was filtered, washed with acetone, and dried under reduced pressure to yield 18 fumarate as the 1 : 1 salt (187 mg, 64%). !
  • fumaric acid (408 mg, 3.5 mmol, 0.8 equiv) was added to a sealed tube containing acetone (20 mL). The solution was carefully heated until all of the fumaric acid dissolved. After cooling the solution to room temperature, a solution of 18 free base (1.028 mg, 4.4 mmol, 1.0 equiv) in acetone (5 mL) was added dropwise, and the mixture was cooled in the freezer overnight. The solid was filtered, washed with acetone, and dried under reduced pressure to yield 18 fumarate as the 1 : 1 salt (1.055 g, 69%).
  • Fraction-2 is the above left compound by 1 1 1 NMR whereas fraction- 1 is the above right compound.
  • fraction- 1 was # (left) by 1 1 1 NMR whereas fraction-2 is # (right).
  • TBG In addition to promoting dendritic growth, TBG also increases dendritic spine density to a comparable extent as ibogaine in mature cortical cultures (DIV20) (FIG. 3E and FIG. 3F).
  • spines were imaged on specific dendritic loci defined by their relation to blood vessel and dendritic architectures.
  • the animals were systemically administered vehicle, TBG, or the hallucinogenic 5-HT2A agonist 2,5- dimethoxy-4-iodoamphetamine (DOI).
  • DOI 2,5- dimethoxy-4-iodoamphetamine
  • ibogaine hydrochloride IBO
  • noribogaine NOR
  • Other chemicals were purchased from commercial sources such as ketamine hydrochloride (KET, Fagron), ketanserin (KETSN, ApexBio), eugenol (Tokyo Chemical Industries), and 5-hydroxytryptamine (Sigma-Aldrich).
  • the fumarate salt of 5- m e t h o x y - /V, /V- d i m e t h y 11 ry p t a m i n e (2: 1, 5-MeO-DMT:fumaric acid) was synthesized in house as described previously and judged to be analytically pure based on NMR and LC-MS data.
  • VEFI 0.1% (agonist studies) or 0.2% (antagonist studies) molecular biology grade dimethyl sulfoxide (Sigma-Aldrich).
  • VEH USP grade saline (0.9%). Free bases were used for all cellular experiments while the fumarate salts of ibogainalog and tabemanthalog were used for the in vivo studies.
  • Dendritogenesis Experiments. For the dendritogenesis experiments conducted using cultured cortical neurons, timed pregnant Sprague Dawley rats were obtained from Charles River Laboratories (Wilmington, MA). Full culturing, staining, and analysis details were performed as previously described. (Dunlap, L. et al., 2019)
  • HTR Head-Twitch Response
  • HEK-293 cells stably expressing hKvl 1.1 (hERG) under G418 selection were a generous gift from Craig January (University of Wisconsin, Madison). Cells were cultured in DMEM containing 10% fetal bovine serum, 2 mM glutamine, 1 mM sodium pyruvate, 100 U/mL penicillin, 100 pg/mL streptomycin, and 500 mg/ml G418.
  • a 2-step pulse (applied every 10 sec) from -80 mV first to 40 mV for 2 sec and then to -60 mV for 4 sec, was used to elicit hERG currents.
  • the percent reduction of tail current amplitude by the drugs was determined and data are shown as mean +/- SD.
  • solutions of the drugs were prepared fresh from 10 mM stock solutions in DMSO. The final DMSO concentration never exceeded 1%.
  • HEK Flp-In 293 T-Rex stable cell lines every plate included a positive control such as 5-HT (for all 5-HT receptors), DADLE (DOR), salvinorin A (KOR), and DAMGO (MOR).
  • 5-HT for all 5-HT receptors
  • DADLE DOR
  • KOR salvinorin A
  • MOR DAMGO
  • 5-HT1D For measurement of 5-HT1D, 5-HT1E, 5-HT4, and 5- HT5A functional assays, -arrestin2 recruitment was measured by the Tango assay utilizing HTLA cells expressing TEV fused- -arrestin2, as described previously with minor modifications. Data for all assays were plotted and non-linear regression was performed using“log(agonist) vs. response” in Graphpad Prism to yield Emax and EC50 parameter estimates.
  • 5HT 2A Sensor Assays HEK293T (ATCC) 5HT2A sensor stable line (sLightl .3s) was generated via lentiviral transduction of HIV-EFla-sLightl.3 and propagated from a single colony. Lentivirus was produced using 2 nd generation lentiviral plasmids pHIV-EFl a - sLightl .3, pHCMV-G, and pCMV-deltaR8.2.
  • sLightl .3s cells were plated in 96-well plates at a density of 40000 24-hours prior to imaging.
  • compounds solubilized in DMSO were diluted from the 1 OOmM stock solution to working concentrations of ImM, IOOmM and lpM with a DMSO concentration of 1%.
  • cells growing in DMEM were washed 2x with HBSS (Gibco) and in agonist mode 180pL of HBSS or in antagonist mode 160pL of HBSS was added to each well after the final wash.
  • Imaging was performed using the Leica DMi8 inverted microscope with a 40x objective using the FITC preset with an excitation of 460nm and emission of 512-542nm.
  • the cellular membrane where the 5HT2A sensor is targeted was autofocused using the adaptive focus controls and 5 images from different regions within the well were taken with each image processed from a 2x2 binning.
  • dFF (Fsat - Fapo)/ Fapo
  • Inactivation score (dFFF(Compound+5HT) - dFF(5HT))/dFF(5HT)
  • mice were anesthetized with an intraperitoneal (i.p.) injection of a mixture of ketamine (87 mg/kg) and xylazine (8.7 mg/kg).
  • ketamine 87 mg/kg
  • xylazine 8.7 mg/kg
  • a small region of the exposed skull was manually thinned down to 20- 30 pm for optical access.
  • mice After handling and habituation to the experimenter, drug-nai ' ve mice first underwent a pretest swim to more reliably induce a depressive phenotype in the subsequent FST sessions. Immobility scores for all mice were determined after the pre-test and mice were randomly assigned to treatment groups to generate groups with similar average immobility scores to be used for the following two FST sessions. The next day, the animals received intraperitoneal injections of TBG (50 mg/kg), a positive control (ketamine, 3 mg/kg), or vehicle (saline). The following day, the animals were subjected to the FST and then returned to their home cages. One week later, the FST was performed again to assess the sustained effects of the drugs. All FSTs were performed between the hours of 8 am and 1 pm. Experiments were video-recorded and manually scored offline. Immobility time— defined as passive floating or remaining motionless with no activity other than that needed to keep the mouse’s head above water— was scored for the last 4 min of the 6 min trial.
  • mice Male C57/BL6J mice (6-8 weeks old) were obtained from The Jackson Laboratory (Bar Harbor, ME) and were individually housed in a reverse light/dark cycle room (lights on 10:00pm-10:00am). Temperature was kept constant at 22 ⁇ 2°C, and relative humidity was maintained at 50 ⁇ 5%. Mice were given access to food and tap water ad libitum. After one week of habituation to the vivarium, the two-bottle choice alcohol-drinking paradigm was conducted as described previously. For 7 weeks, mice were given intermittent access in their home cage to alcohol. On Mondays, Wednesdays, and Fridays, two bottles were made available for 24 h— one containing 20% ethanol and another containing only water.
  • mice were administered TBG (50 mg/kg) or vehicle (saline) via intraperitoneal injection 3 h before the beginning of a drinking session. Ethanol (g/kg) and water (ml/kg) intake were monitored during the first 4 h (initial binge), the first 24 h, and the second 24 h. Next, the animals were only given water for 48 h before the start of another drinking session when ethanol and water consumption was monitored. The placement (right or left) of the bottles was altered in each session to control for side preference. Spillage was monitored using an additional bottle in a nearby unused cage. Alcohol preference was calculated as the ratio between alcohol/(water + alcohol). Mice were tested using a counterbalanced, within subject design with one week of drug-free alcohol drinking regimen between treatments. One mouse was excluded because the bottle was leaking.

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Abstract

The present invention provides azepino-indoles and other heterocycles and methods of using the compounds for treating brain disorders.

Description

AZEPINO-INDOLES AND OTHER HETEROCYCLES FOR TREATING BRAIN
DISORDERS
CROSS-REFERENCES TO RELATED APPLICATIONS
[0001] This application claims priority to U.S. Provisional Application No. 62/811,208 filed February 27, 2019, which is incorporated herein by reference in its entirety for all purposes.
STATEMENT AS TO RIGHTS TO INVENTIONS MADE UNDER FEDERALLY SPONSOERED RESEARCH AND DEVELOPMENT
[0002] This invention was made with Government support under Grant No.
R01GM 128997 awarded by the National Institutes of Health. The Government has certain rights in this invention.
BACKGROUND OF THE INVENTION
[0003] The psychedelic alkaloid ibogaine has robust anti-addictive properties in the clinic and in animal models. Ibogaine possesses the potential to treat patients addicted to a variety of substances, for example, including opiates, psychostimulants, alcohol, and nicotine.
Moreover, its therapeutic effects are long lasting, which has been attributed to its ability to modify addiction-related neural circuitry through activation of neurotrophic factor signaling. Ibogaine reduces symptoms of drug withdrawal, reduces drug cravings, and prevents relapse. In rodents, ibogaine reduces drug self-administration and prevents drug-induced dopamine release in several brain regions. However, several safety concerns have hindered the clinical development of ibogaine, including, for example, its toxicity, hallucinogenic potential, and proclivity for inducing cardiac arrhythmias via hERG channel inhibition.
[0004] Ibogaine increases glial cell line-derived neurotrophic factor (GDNF) expression in the ventral tegmental area (VTA), and intra-VTA infusion of ibogaine reduces alcohol seeking behavior in rodents. Ibogaine impacts brain-derived neurotrophic factor (BDNF) and GDNF signaling in multiple brain regions implicated in the pathophysiology of addiction. Noribogaine, an active metabolite of ibogaine, is a potent psychoplastogen capable of increasing cortical neuron dendritic arbor complexity. Other psychoplastogens, such as lysergic acid diethylamide (LSD) and psilocin (the active metabolite of psilocybin) have anti- addictive properties in the clinic similar to ibogaine. The ability of psychoplastogens to promote structural and functional neural plasticity in addiction-related circuitry can explain their abilities to reduce drug-seeking behavior for weeks to months following a single administration. Moreover, by modifying neural circuitry rather than simply blocking the targets of a particular addictive substance (e.g., opioid receptors, nicotinic receptors, etc.), psychoplastogens like ibogaine have the potential to be broadly applicable anti-addictive agents.
[0005] What is needed are new compounds lacking ibogaine’ s toxicity and hallucinogenic effects but maintain therapeutic efficacy. Surprisingly, the present invention meets this and other needs.
BRIEF SUMMARY OF THE INVENTION
[0006] In one embodiment, the present invention provides a compound of Formula I:
Figure imgf000003_0001
wherein: R1 is hydrogen or Ci-6 alkyl; R3a and R3b are each independently hydrogen, Ci-6 alkyl, Cvx cycloalkyl, or C4-14 alkyl-cycloalkyl; R3c is hydrogen or Ci-6 alkyl; alternatively,
R2 and R3b are combined with the atoms to which they are attached to form a C5- 8 heterocycloalkyl substituted with 1 to 3 R9 groups which are each independently hydrogen, Ci-6 alkyl, C s cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl- heterocycloalkyl, Ce-i2 aryl, C7-18 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R9 groups attached to the same atom are combined to form =0; alternatively, R2 and R3c are combined with the atoms to which they are attached to form a Cvs cycloalkyl, substituted with 1 to 3 R10 groups which are each independently hydrogen, Ci-6 alkyl, C s cycloalkyl, C3- 14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, C6-12 aryl, C7-18 alkyl- aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R10 groups attached to the same atom are combined to form =0; R4, R5, R6 and R7 are each independently hydrogen, Ci-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, halogen, Ci-6 haloalkyl, Ci-6 alkylamine, Ci-6 alkoxy, Ci-6 haloalkoxy, -
Figure imgf000003_0002
N(R8b)C(0)N(R8cR8d), -C(0)C(0)N(R8bR8c), -S(02)R8b, -S(0)2N(R8bR8c), C3-s cycloalkyl, C3- 14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, C6-i2 aryl, C7-I8 alkyl- aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, wherein at least one of R4, R5, R6 and R7 is not H; alternatively, R4 and R5, R5 and R6, or R6 and R7 are combined with the atoms to which they are each attached to form a C3-6 cycloalkyl, C3-6 heterocycloalkyl, Ce-n aryl or C5-10 heteroaryl; R8a, R8b, R8c and R8d are each independently H, Ci-6 alkyl; wherein when R2 and R3b are combined with the atoms to which they are attached to form a C7 heterocycloalkyl, and R3a is methyl, then R5 is not OMe, OH, or Cl; wherein when R2 and R3b are combined with the atoms to which they are attached to form a C7 heterocycloalkyl, and R3a is ethyl, then R6 is not OMe; wherein when R2 and R3b are combined with the atoms to which they are attached to form a C7 heterocycloalkyl, and R6 is methyl, Cl, F, or -OMe, then at least one of R4, R5, and R7 is not hydrogen; wherein when R2 and R3b are combined with the atoms to which they are attached to form a CV, heterocycloalkyl, and R6 is Ci-6 alkyl, halogen, Ci-6 alkyoxy, -C(0)H, -NH2, then at least one of R4, R5, and R7 is not hydrogen; and wherein when R2 and R3b are combined with the atoms to which they are attached to form a C6 heterocycloalkyl, and R6 is methyl, halogen, or -C(0)H, then at least one of R4, R5, and R7 is not methyl or Cl, or pharmaceutically acceptable salts and isomers thereof.
[0007] In another embodiment, the present invention provides a compound, or a pharmaceutically acceptable salt thereof, wherein the compound is
Figure imgf000004_0001
[0008] In another embodiment, the present invention provides a pharmaceutical composition comprising a compound of the present invention, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient.
[0009] In another embodiment, the present invention provides a method for increasing neuronal plasticity, comprising contacting a neuronal cell with a compound of Formula I:
Figure imgf000005_0001
or a pharmaceutically acceptable salt thereof, in an amount sufficient to increase neuronal plasticity of the neuronal cell, wherein: R1 is hydrogen or Ci-6 alkyl; R3a and R3b are each independently hydrogen, Ci-6 alkyl, C vs cycloalkyl, or C4-14 alkyl-cycloalkyl; R3c is hydrogen or Ci-6 alkyl; alternatively, R2 and R3b are combined with the atoms to which they are attached to form a C5-8 heterocycloalkyl substituted with 1 to 3 R9 groups which are each independently hydrogen, Ci-6 alkyl, C3-8 cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10
heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-18 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R9 groups attached to the same atom are combined to form =0; alternatively, R2 and R3c are combined with the atoms to which they are attached to form a C5-8 cycloalkyl, substituted with 1 to 3 R10 groups which are each independently hydrogen, Ci-6 alkyl, C s cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl- heterocycloalkyl, Ce-n aryl, C7-18 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R10 groups attached to the same atom are combined to form =0; R4, R5, R6 and R7 are each independently hydrogen, Ci-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, halogen, Ci-6 haloalkyl, Ci-6
Figure imgf000005_0002
C3-8 cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-18 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, wherein at least one of R4, R5, R6 and R7 is not H; alternatively, R4 and R5, R5 and R6, or R6 and R7 are combined with the atoms to which they are each attached to form a C3-6 cycloalkyl,
C3-6 heterocycloalkyl, Ce-n aryl or C5-10 heteroaryl; and R8a, R8b, R8c and R8d are each independently H, Ci-6 alkyl. [0010] In another embodiment, the present invention provides a method of treating a brain disorder, comprising administering to a subject in need thereof a therapeutically effective amount of a compound of Formula I:
Figure imgf000006_0001
or a pharmaceutically acceptable salt thereof, thereby treating the brain disorder, wherein: R1 is hydrogen or Ci-6 alkyl; R3a and R3b are each independently hydrogen, Ci-6 alkyl, CYx cycloalkyl, or C4-14 alkyl-cycloalkyl; R3c is hydrogen or Ci-6 alkyl; alternatively, R2 and R3b are combined with the atoms to which they are attached to form a C5-8 heterocycloalkyl substituted with 1 to 3 R9 groups which are each independently hydrogen, Ci-6 alkyl, C s cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-18 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R9 groups attached to the same atom are combined to form =0; alternatively, R2 and R3c are combined with the atoms to which they are attached to form a C5-8 cycloalkyl, substituted with 1 to 3 R10 groups which are each independently hydrogen, Ci-6 alkyl, C3-8 cycloalkyl, C3-14 alkyl-cycloalkyl, C4- 10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-18 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R10 groups attached to the same atom are combined to form =0; R4, R5, R6 and R7 are each independently hydrogen, Ci-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, halogen, Ci-6 haloalkyl, Ci-6 alkylamine, Ci-6 alkoxy, Ci-6 haloalkoxy, -OR8a, -NO2, -CN, -
Figure imgf000006_0002
C(0)C(0)N (R8bR8c), -S(02)R8b, -S(0)2N(R8bR8c), C3-8 cycloalkyl, C3-14 alkyl-cycloalkyl, C - 10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-I8 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, wherein at least one of R4, R5, R6 and R7 is not H; alternatively, R4 and R5, R5 and R6, or R6 and R7 are combined with the atoms to which they are each attached to form a C3-6 cycloalkyl, C3-6 heterocycloalkyl, Ce-n aryl or C5-10 heteroaryl; and R8a, R8b,
R8c and R8d are each independently H, Ci-6 alkyl.
[0011] In another embodiment, the present invention provides a method for increasing at least one of translation, transcription or secretion of neurotrophic factors, comprising contacting a neuronal cell with a compound of Formula I:
Figure imgf000007_0001
or a pharmaceutically acceptable salt thereof, in an amount sufficient to increase neuronal plasticity of the neuronal cell, wherein: R1 is hydrogen or Ci-6 alkyl; R3a and R3b are each independently hydrogen, Ci-6 alkyl, Cvs cycloalkyl, or C4-14 alkyl-cycloalkyl; R3c is hydrogen or Ci-6 alkyl; alternatively, R2 and R3b are combined with the atoms to which they are attached to form a C5-8 heterocycloalkyl substituted with 1 to 3 R9 groups which are each independently hydrogen, Ci-6 alkyl, C3-8 cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10
heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-I8 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R9 groups attached to the same atom are combined to form =0; alternatively, R2 and R3c are combined with the atoms to which they are attached to form a C5-8 cycloalkyl, substituted with 1 to 3 R10 groups which are each independently hydrogen, Ci-6 alkyl, C s cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl- heterocycloalkyl, Ce-n aryl, C7-18 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R10 groups attached to the same atom are combined to form =0; R4, R5, R6 and R7 are each independently hydrogen, Ci-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, halogen, Ci-6 haloalkyl, Ci-6
Figure imgf000007_0002
C3-8 cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-I8 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, wherein at least one of R4, R5, R6 and R7 is not H; alternatively, R4 and R5, R5 and R6, or R6 and R7 are combined with the atoms to which they are each attached to form a C3-6 cycloalkyl,
C3-6 heterocycloalkyl, Ce-n aryl or C5-10 heteroaryl; and R8a, R8b, R8c and R8d are each independently H, Ci-6 alkyl.
BRIEF DESCRIPTION OF THE DRAWINGS
[0012] FIG. 1A to FIG. IB show function-oriented synthesis of ibogalogs. FIG. 1 A shows key structural features of ibogaine and related alkaloids. Deletion of either the isoquinuclidine or tetrahydroazepine leads to simplified ibogaine analogs. FIG. IB shows synthesis of tetrahydroazepine-containing ibogalogs.
[0013] FIG. 2A to FIG.2K show tabemanthalog (TBG) is a safer analog of iboga alkaloids. FIG. 2A shows the design of ibogainalog (IBG) and TBG, simplified analogs of ibogaine and tabemanthine, respectively. FIG. 2B shows mouse HTR assays demonstrate that TBG is not hallucinogenic. The doses (mg/kg) of IBG and TBG are indicated. +Ctrl = 5-MeO-DMT (10 mg/kg). FIG. 2C shows solubility of various drugs in a 0.9% saline solution. FIG. 2D shows inhibition of hERG channels expressed in HEK293 cells. FIG. 2E shows that unlike ibogaine, IBG and TBG do not induce bradycardia in larval zebrafish. FIG. 2F shows ratio of atrium to ventricle beats per minute (BPM). Deviations from 1 indicate an increased risk of arrhythmias. Sertindole (SI) was used as a positive control. FIG. 2G shows confusion matrix for classification of IBO, NOR, IBG, and TBG (200 mM), plus VEH and lethal controls. Cells show the proportion of classification between true labels (Y-axis) and predicted labels (X- axis). FIG. 2H shows representative images of zebrafish treated with compounds (100 mM) for 2 dpf. Scale bar = 1 mm. FIG. 21 shows proportion of viable and non- viable (malformed or dead) zebrafish following treatment with compounds (100 pM). FIG. 2J shows the effects of compound treatment on malformation and death over time. FIG. 2K shows agonist and antagonist activities at the 5-HT2A and 5-HT2B receptors as measured by Gq-mediated calcium flux. Data represent percent 5-HT fold-over-basal response performed from at least three independent experiments performed in duplicate. IBO = ibogaine, NOR = noribogaine.
[0014] FIG. 3A to FIG. 31 show TBG promotes neural plasticity. FIG. 3A shows representative images of rat embryonic cortical neurons (DIV6) treated with TBG or VEH. Scale bar = 10 pm. FIG. 3B shows Sholl analysis of TBG and VEH treated neurons. The light shadings represent 95% confidence intervals. FIG. 3C shows maximum numbers of crossings (Nmax) of the Sholl plot in b demonstrate that TBG increases dendritic arbor complexity. FIG. 3D shows the effects of TBG on dendritic growth are blocked by the 5- HT2A antagonist ketanserin (KETSN). FIG. 3E shows representative images of secondary branches of rat embryonic cortical neurons (DIV20) after treatment with ibogalogs for 24 h. Scale bar = 2 pm. FIG. 3F shows TBG increases dendritic spine density on rat embryonic cortical neurons (DIV20) after treatment for 24 h. KET = ketamine. FIG. 3G shows schematic illustrating the design of in vivo spine dynamics experiments using transcranial 2- photon imaging. FIG. 3H shows representative images of the same dendritic segments from mouse primary sensory cortex before (Day 0) and after (Day 1) treatment with VEH, DOI, or TBG. Blue, red, and white arrowheads represent newly formed spines, eliminated spines, and filopodia, respectively. Scale bar = 2 pm. FIG. 31 shows quantification of spine dynamics reveals that DOI and TBG increase spine formation but have no effect on spine elimination.
[0015] FIG. 4A to FIG. 4E show TBG has antidepressant and anti-addictive properties.
FIG. 4A shows schematic illustrating the design of FST experiments. Mice were subjected to a pretest, dosed with compounds, and then retested at 24 h and 7 days following drug administration. FIG. 4B shows quantification of immobility reveals that TBG has antidepressant-like effects. FIG. 4C shows timeline of binge-drinking experiment. Bottles of 20% EtOFI (white droplets) and FEO (blue droplets) were available for 24 h periods every 1- 2 days for 7 weeks. Between drinking sessions, two bottles of FEO were provided. FIG. 4D shows TBG reduces EtOFI consumption and preference during a binge drinking session without impacting FEO intake. FIG. 4E TBG administration results in long-lasting decreases in EtOH consumption.
[0016] FIG. 5A to FIG. 5B show the effects of ibogalogs on dendritogenesis. FIG. 5A shows representative images of rat embryonic cortical neurons (DIV6) treated with compounds. Scale bar = 10 pm. FIG. 5B shows maximum numbers of crossings (Nmax) of the Sholl plots demonstrate that tetrahydroazepine-containing ibogalogs are more effective at increasing dendritic arbor complexity than are isoquinuclicine-containing ibogalogs.
[0017] FIG. 6 shows several ibogalogs promote dendritogenesis. Sholl analysis (circle radii = 1.34 pm increments) demonstrates that cultured cortical neurons treated with several ibogalogs have more complex dendritic arbors as compared to vehicle control (n = 52-83 neurons per treatment). The shaded area surrounding each line represents 95% confidence intervals. Control compounds, isoquinuclidines, and tetrahydroazepines are shown in blue, purple, and red, respectively.
[0018] FIG. 7 shows ibogaine hydrochloride exhibits limited solubility in various saline- based vehicles. Solutions of saline (0.9%) containing various percentages of co
solvents/additives were added to finely crushed ibogaine hydrochloride. All attempts to improve its solubility through pulverizing, sonication, and mild heating (< 50 °C) were unsuccessful. Moreover, the addition of co-solvents (ethanol, dimethyl sulfoxide, glycerol), surfactants (Kolliphor), or hydrotropes (ATP) to the vehicle did not substantially improve its solubility. It was confirmed the purity and identity of the ibogaine hydrochloride used in these studies through a combination of NMR, LC-MS, and X-ray crystallography experiments.
[0019] FIG. 8 shows heatmaps representing aggregate larval zebraflsh locomotor activity per well compared to vehicle controls (pseudo-Z-score). Red and blue indicate higher and lower activity than the mean of vehicle controls, respectively, while white indicates activity within ± 1 SD from control. Stimuli applied over time are indicated under the heatmaps. Colors indicate bright LED light of respective colors, black traces represent the waveforms of acoustic stimuli, and gray vertical lines indicate physical tapping as secondary acoustic stimuli.
[0020] FIG. 9 shows increasing concentrations of IBG and TBG do not produce movement responses in larval zebraflsh similar to a lethal concentration of eugenol (100 mM).
Concentration-response curves for compounds subjected to the battery of stimuli shown in FIG. 8. Response is inversely proportional to classification accuracy; 0-20% indicates no ability and 100% indicates perfect classification. Lower percentages indicate treatments that were more often classified as vehicle (blue) or lethal (red). The solid line denotes the median and the shading denotes a 95th percentile confidence interval calculated by bootstrap. N = 8 wells / condition (64 animals / condition).
[0021] FIG. 10 shows TBG does not induce seizures in larval zebraflsh. Transgenic larval zebraflsh expressing GCaMP5G were immobilized in agarose, treated with compounds, and imaged over time. The known seizure-inducing compound PTZ was used as a positive control. All compounds were treated at 200 pM (n = 2 per condition).
[0022] FIG. 1 1A and FIG. 1 IB show TBG (66 pM) does not cause developmental toxicity in zebrafish. FIG. 11 A shows proportion of viable and non- viable (malformed + dead) zebraflsh following treatment with VEH and TBG (66 pM) for 5 dpf (Fisher’s exact test: p = 0.3864) FIG. 1 IB shows representative images of zebrafish treated with VEH and TBG (66 pM) for 2 and 5 dpf. Scale bar = 2 mm.
[0023] FIG. 12 shows concentration-response curves demonstrating the abilities of ibogalogs and related compounds to activate 5-HT and opioid receptors. All compounds were assayed in parallel using the same drug dilutions. Graphs reflect representative
concentration-response curves plotting mean and S.E.M of data points performed in duplicate or triplicate. Assay details are described in the methods. [0024] FIG. 13 shows pharmacological profiles ofibogalogs and related compounds. Table showing EC50 and Emax estimates from at least two independent concentration-response curves performed in duplicate or triplicate. Log(Emax/EC5o) activity is included as an estimate of system agonist activity. ; Inactive = inactive in agonist mode; N.D. = not determined; blue boxes = exhibits antagonist activity; dark grey boxes = inactive in agonist mode but not tested in antagonist mode; orange boxes indicate inverse agonist.
[0025] FIG. 14 shows screening of DMT derivatives and ibogalogs reveals differences in 5-HT receptorome profiles. Ibogalogs are more selective 5-HT2A agonists than 5-MeO-DMT.
[0026] FIG. 15 shows the dose response profile of hallucinogenic and non-hallucinogenic compounds to a 5HT2A sensor assay in agonist mode.
[0027] FIG. 16 shows the response profile of hallucinogenic and non-hallucinogenic compounds to a 5HT2A sensor assay in agonist mode.
[0028] FIG. 17 shows the dose response profile of 5HT, 6-MeO-DMT (FIG. 17A), and lisuride (FIG. 17B) to a 5HT2A sensor assay in antagonist mode.
[0029] FIG. 18 shows the response profile of hallucinogenic and non-hallucinogenic compounds to a 5HT2A sensor assay in antagonist mode.
DETAILED DESCRIPTION OF THE INVENTION
I. General
[0030] Ibogaine has garnered attention as a potential plasticity-promoting anti-addictive agent; a single dose of this hallucinogenic compound has demonstrated sustained efficacy for treating addiction to alcohol, opiates, nicotine, and psychostimulants. An analog of ibogaine capable of promoting fibroblast growth factor 2 (FGF2)-induced GDNF release in C6 glioma cells was developed; however, the effects of this compound on neural plasticity are not known.
[0031] Ibogaine is highly plasticity-promoting. However, ibogaine’s hallucinogenic effects present barriers to regulatory approval and severely limits its therapeutic potential. It is possible to decouple the hallucinogenic effects of a drug from its ability to promote neural plasticity. Compounds described herein, lacking the isoquinuclidine of ibogaine and transposing the methoxy group from the 5- to the 6-position of the indole, are non- hallucinogenic ibogaine derivatives that, for example promote neuronal growth. [0032] Compounds capable of modifying neural circuits that control motivation, anxiety, and drug-seeking behavior have potential for treating depression, post-traumatic stress disorder (PTSD), and substance abuse disorder (SUD). In some instances, such
psychoplastogenic medicines produce sustained therapeutic effects because the medicines treat the underlying pathological changes in circuitry. Psychedelic compounds have distinguished themselves in this regard because, for example, they promote structural and functional neural plasticity in key circuits, elicit therapeutic responses in multiple neuropsychiatric disorders, and produce beneficial effects that last for months following a single administration.
[0033] Described herein are water soluble, non-hallucinogenic, analogs of ibogaine.
Despite lacking hallucinogenic properties, compounds described herein promote structural neural plasticity, reduce alcohol consumption, and produce antidepressant-like effects in mice.
[0034] In some instances, hallucinogenic 5-HT2A agonists (e.g., DMT, LSD, DOI, etc.) are useful for treating neurological diseases, such as neuropsychiatric diseases. (Ly et al., 2018.) However, the hallucinogenic and dissociative potential of such compounds has limited the use of these compounds in the clinic. 5-HT2A antagonists abrogate the neuritogenesis and spinogenesis effects of hallucinogenic compounds with 5-HT2A agonist activity, e.g., DMT, LSD, and DOI, demonstrating the correlation of 5-HT2A agonism and the promotion of neural plasticity (Ly et al., 2018; Dunlap et al., 2020).
[0035] Provided herein in some embodiments are non-hallucinogenic psychoplastogens. In some embodiments, the non-hallucinogenic ibogaine analogs described herein have improved physiochemical properties as a result of, for example, decreasing total polar surface area. Described herein in some embodiments are non-hallucinogenic compounds that demonstrate similar therapeutic potential as hallucinogenic 5-HT2A agonists. In some embodiments, the non-hallucinogenic compounds described herein provide better therapeutic potential than hallucinogenic 5-HT2A modulators for neurological diseases.
[0036] The present invention provides azepino-indoles and other heterocyclic non- hallucinogenic compounds useful for the treatment of a variety of brain disorders and other conditions, as well as increasing neuronal plasticity, or increasing at least one of translation, transcription, or secretion of neurotrophic factors. II. Definitions
[0037] Unless specifically indicated otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the art to which this invention belongs. In addition, any method or material similar or equivalent to a method or material described herein can be used in the practice of the present invention. For purposes of the present invention, the following terms are defined.
[0038] “A,”“an,” or“the” not only include aspects with one member, but also include aspects with more than one member. For instance, the singular forms“a,”“an,” and“the” include plural referents unless the context clearly dictates otherwise. Thus, for example, reference to“a cell” includes a plurality of such cells and reference to“the agent” includes reference to one or more agents known to those skilled in the art, and so forth.
[0039] Abbreviations. VEH = vehicle; KET = ketamine; IBO = ibogaine; NOR = noribogaine; IBG = ibogainalog; TBG = tabemanthalog; KETSN = ketanserin; SI = sertindole; DOI = 2,5-dimethoxy-4-iodoamphetamine; FST = forced swim test; EtOH = ethanol, DMSO = dimethyl sulfoxide, ATP = adenosine triphosphate.
[0040] “Alkyl” refers to a straight or branched, saturated, aliphatic radical having the number of carbon atoms indicated. Alkyl can include any number of carbons, such as C1-2, Cl-3, Cl-4, Cl-5, Cl-6, Cl-7, Cl-8, Cl-9, Cl-10, C2-3, C2-4, C2-5, C2-6, C3-4, C3-5, C3-6, C4-5, C4-6 and C5-6. For example, Ci-6 alkyl includes, but is not limited to, methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec-butyl, tert-butyl, pentyl, isopentyl, hexyl, etc. Alkyl can also refer to alkyl groups having up to 20 carbons atoms, such as, but not limited to heptyl, octyl, nonyl, decyl, etc. Alkyl groups can be substituted or unsubstituted.
[0041] “Alkylene” refers to a straight or branched, saturated, aliphatic radical having the number of carbon atoms indicated, and linking at least two other groups, i.e., a divalent hydrocarbon radical. The two moieties linked to the alkylene can be linked to the same atom or different atoms of the alkylene group. For instance, a straight chain alkylene can be the bivalent radical of -(CFE , where n is 1, 2, 3, 4, 5 or 6. Representative alkylene groups include, but are not limited to, methylene, ethylene, propylene, isopropylene, butylene, isobutylene, sec-butylene, pentylene and hexylene. Alkylene groups can be substituted or unsubstituted. [0042] “Alkenyl” refers to a straight chain or branched hydrocarbon having at least 2 carbon atoms and at least one double bond. Alkenyl can include any number of carbons, such as C2, C2-3, C2-4, C2-5, C2-6, C2-7, C2-8, C2-9, C2-10, C3, C3-4, C3-5, C3-6, C4, C4-5, C4-6, C5, C5-6, and Ce. Alkenyl groups can have any suitable number of double bonds, including, but not limited to, 1, 2, 3, 4, 5 or more. Examples of alkenyl groups include, but are not limited to, vinyl (ethenyl), propenyl, isopropenyl, 1-butenyl, 2-butenyl, isobutenyl, butadienyl, 1-pentenyl, 2-pentenyl, isopentenyl, 1,3-pentadienyl, 1,4-pentadienyl, 1-hexenyl, 2-hexenyl, 3-hexenyl, 1,3-hexadienyl, 1 ,4-hexadienyl, 1,5-hexadienyl, 2,4-hexadienyl, or
1,3,5-hexatrienyl. Alkenyl groups can be substituted or unsubstituted.
[0043] “Alkynyl” refers to either a straight chain or branched hydrocarbon having at least 2 carbon atoms and at least one triple bond. Alkynyl can include any number of carbons, such as C2, C2-3, C2-4, C2-5, C2-6, C2-7, C2-8, C2-9, C2-10, C3, C3-4, C3-5, C3-6, C4, C4-5, C4-6, C5, C5-6, and Ce. Examples of alkynyl groups include, but are not limited to, acetylenyl, propynyl, 1-butynyl, 2-butynyl, butadiynyl, 1-pentynyl, 2-pentynyl, isopentynyl, 1,3-pentadiynyl,
1.4-pentadiynyl, 1-hexynyl, 2-hexynyl, 3-hexynyl, 1,3-hexadiynyl, 1 ,4-hexadiynyl,
1.5-hexadiynyl, 2,4-hexadiynyl, or 1,3,5-hexatriynyl. Alkynyl groups can be substituted or unsubstituted.
[0044] “Cycloalkyl” refers to a saturated or partially unsaturated, monocyclic, bicyclic, fused bicyclic or bridged polycyclic ring assembly containing from 3 to 12 ring atoms, or the number of atoms indicated. Cycloalkyl can include any number of carbons, such as C3-6,
C4-6, C5-6, C3-8, C4-8, C5-8, Ce-8, C3-9, C3-10, C3-11, and C3-12. Saturated monocyclic cycloalkyl rings include, for example, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, and cyclooctyl. Bicyclic compounds include spirocyclic compounds, fused bicyclic compounds and bridged bicyclic compounds. Saturated bicyclic and polycyclic cycloalkyl rings include, for example, norbomane, [2.2.2] bicyclooctane, decahydronaphthalene and adamantane. Cycloalkyl groups can also be partially unsaturated, having one or more double or triple bonds in the ring. Representative cycloalkyl groups that are partially unsaturated include, but are not limited to, cyclobutene, cyclopentene, cyclohexene, cyclohexadiene (1,3- and 1,4-isomers), cycloheptene, cycloheptadiene, cyclooctene, cyclooctadiene (1,3-, 1,4- and 1,5-isomers), norbomene, and norbomadiene. When cycloalkyl is a saturated monocyclic CYx cycloalkyl, exemplary groups include, but are not limited to cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl and cyclooctyl. When cycloalkyl is a saturated monocyclic C3-6 cycloalkyl, exemplary groups include, but are not limited to cyclopropyl, cyclobutyl, cyclopentyl, and cyclohexyl. Cycloalkyl groups can be substituted or unsubstituted.
[0045] “Alkyl-cycloalkyl” refers to a radical having an alkyl component and a cycloalkyl component, where the alkyl component links the cycloalkyl component to the point of attachment. The alkyl component is as defined above, except that the alkyl component is at least divalent, an alkylene, to link to the cycloalkyl component and to the point of attachment. In some instances, the alkyl component can be absent. The alkyl component can include any number of carbons, such as Ci-6, C1-2, C1-3, CM, C1-5, C2-3, C2-4, C2-5, C2-6, C3-4, C3-5, C3-6,
C4-5, C4-6 and C5-6. The cycloalkyl component is as defined within. Exemplary alkyl- cycloalkyl groups include, but are not limited to, methyl-cyclopropyl, methyl-cyclobutyl, methyl-cyclopentyl and methyl-cyclohexyl.
[0046] “Heterocycloalkyl” refers to a cycloalkyl as defined above, having from 3 to 12 ring members and from 1 to 4 heteroatoms of N, O and S. Heterocycloalkyl includes bicyclic compounds which include a heteroatom. Bicyclic compounds includes spirocyclic compounds, fused bicyclic compounds, and bridged bicyclic compounds The heteroatoms can also be oxidized, such as, but not limited to, -S(O)- and -S(0)2-. Heterocycloalkyl groups can include any number of ring atoms, such as, 3 to 6, 4 to 6, 5 to 6, 3 to 8, 4 to 8, 5 to 8,
6 to 8, 3 to 9, 3 to 10, 3 to 11, or 3 to 12 ring members. Any suitable number of heteroatoms can be included in the heterocycloalkyl groups, such as 1, 2, 3, or 4, or 1 to 2, 1 to 3, 1 to 4, 2 to 3, 2 to 4, or 3 to 4. The heterocycloalkyl group can include groups such as aziridine, azetidine, pyrrolidine, piperidine, azepane, azocane, quinuclidine, pyrazolidine,
imidazolidine, piperazine (1,2-, 1,3- and 1,4-isomers), oxirane, oxetane, tetrahydrofuran, oxane (tetrahydropyran), oxepane, thiirane, thietane, thiolane (tetrahydrothiophene), thiane (tetrahydrothiopyran), oxazolidine, isoxazolidine, thiazolidine, isothiazolidine, dioxolane, dithiolane, morpholine, thiomorpholine, dioxane, or dithiane. The heterocycloalkyl groups can also be fused to aromatic or non-aromatic ring systems to form members including, but not limited to, indoline. Heterocycloalkyl groups can be unsubstituted or substituted. For example, heterocycloalkyl groups can be substituted with CM alkyl or oxo (=0), among many others.
[0047] “Alkyl-heterocycloalkyl” refers to a radical having an alkyl component and a heterocycloalkyl component, where the alkyl component links the heterocycloalkyl component to the point of attachment. The alkyl component is as defined above, except that the alkyl component is at least divalent, an alkylene, to link to the heterocycloalkyl component and to the point of attachment. The alkyl component can include any number of carbons, such as Co-6, C1-2, C1-3, CM, C1-5, Ci-6, C2-3, C2-4, C2-5, C2-6, C3-4, C3-5, C3-6, C4-5, C4-6 and C5-6. In some instances, the alkyl component can be absent. The heterocycloalkyl component is as defined above. Alkyl-heterocycloalkyl groups can be substituted or unsubstituted.
[0048] “Halogen” refers to fluorine, chlorine, bromine and iodine.
[0049] “Haloalkyl” refers to alkyl, as defined above, where some or all of the hydrogen atoms are replaced with halogen atoms. As for alkyl group, haloalkyl groups can have any suitable number of carbon atoms, such as Ci-6. For example, haloalkyl includes
trifluoromethyl, flouromethyl, etc. In some instances, the term“perfluoro” can be used to define a compound or radical where all the hydrogens are replaced with fluorine. For example, perfluoromethyl refers to 1,1,1 -trifluoromethyl. Alkylamine
[0050] “Alkoxy” refers to an alkyl group having an oxygen atom that connects the alkyl group to the point of attachment: alkyl-O-. As for alkyl group, alkoxy groups can have any suitable number of carbon atoms, such as Ci-6. Alkoxy groups include, for example, methoxy, ethoxy, propoxy, iso-propoxy, butoxy, 2-butoxy, iso-butoxy, sec-butoxy, tert-butoxy, pentoxy, hexoxy, etc. The alkoxy groups can be further substituted with a variety of substituents described within. Alkoxy groups can be substituted or unsubstituted.
[0051] “Haloalkoxy” refers to an alkoxy group where some or all of the hydrogen atoms are substituted with halogen atoms. As for an alkyl group, haloalkoxy groups can have any suitable number of carbon atoms, such as Ci-6. The alkoxy groups can be substituted with 1, 2, 3, or more halogens. When all the hydrogens are replaced with a halogen, for example by fluorine, the compounds are per-substituted, for example, perfluorinated. Haloalkoxy includes, but is not limited to, trifluoromethoxy, 2,2,2,-trifluoroethoxy, perfluoroethoxy, etc.
[0052] “Amine” refers to an -N(R)2 group where the R groups can be hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl, heterocycloalkyl, aryl, or heteroaryl, among others. The R groups can be the same or different. The amino groups can be primary (each R is hydrogen), secondary (one R is hydrogen) or tertiary (each R is other than hydrogen).
[0053] “Alkyl amine” refers to an alkyl group as defined within, having one or more amino groups. The amino groups can be primary, secondary or tertiary. The alkyl amine can be further substituted with a hydroxy group to form an amino-hydroxy group. Alkyl amines useful in the present invention include, but are not limited to, ethyl amine, propyl amine, isopropyl amine, ethylene diamine and ethanolamine. The amino group can link the alkyl amine to the point of attachment with the rest of the compound, be at the omega position of the alkyl group, or link together at least two carbon atoms of the alkyl group. One of skill in the art will appreciate that other alkyl amines are useful in the present invention.
[0054] “Aryl” refers to an aromatic ring system having any suitable number of ring atoms and any suitable number of rings. Aryl groups can include any suitable number of ring atoms, such as, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15 or 16 ring atoms, as well as from 6 to 10, 6 to 12, or 6 to 14 ring members. Aryl groups can be monocyclic, fused to form bicyclic or tricyclic groups, or linked by a bond to form a biaryl group. Representative aryl groups include phenyl, naphthyl and biphenyl. Other aryl groups include benzyl, having a methylene linking group. Some aryl groups have from 6 to 12 ring members, such as phenyl, naphthyl or biphenyl. Other aryl groups have from 6 to 10 ring members, such as phenyl or naphthyl. Some other aryl groups have 6 ring members, such as phenyl. Aryl groups can be substituted or unsubstituted.
[0055] “Alkyl-aryl” refers to a radical having an alkyl component and an aryl component, where the alkyl component links the aryl component to the point of attachment. The alkyl component is as defined above, except that the alkyl component is at least divalent, an alkylene, to link to the aryl component and to the point of attachment. The alkyl component can include any number of carbons, such as Co-6, C1-2, Cm, CM, CM, CM, C2-3, C2-4, C2-5, C2-6, C3-4, C3-5, C3-6, C4-5, C4-6 and C5-6. In some instances, the alkyl component can be absent. The aryl component is as defined above. Examples of alkyl-aryl groups include, but are not limited to, benzyl and ethyl-benzene. Alkyl-aryl groups can be substituted or unsubstituted.
[0056] “Heteroaryl” refers to a monocyclic or fused bicyclic or tricyclic aromatic ring assembly containing 5 to 16 ring atoms, where from 1 to 5 of the ring atoms are a heteroatom such as N, O or S. Heteroaryl groups can include any number of ring atoms, such as, 5 to 6,
3 to 8, 4 to 8, 5 to 8, 6 to 8, 3 to 9, 3 to 10, 3 to 1 1, or 3 to 12 ring members. Any suitable number of heteroatoms can be included in the heteroaryl groups, such as 1, 2, 3, 4, or 5, or 1 to 2, 1 to 3, 1 to 4, 1 to 5, 2 to 3, 2 to 4, 2 to 5, 3 to 4, or 3 to 5. Heteroaryl groups can have from 5 to 8 ring members and from 1 to 4 heteroatoms, or from 5 to 8 ring members and from 1 to 3 heteroatoms, or from 5 to 6 ring members and from 1 to 4 heteroatoms, or from 5 to 6 ring members and from 1 to 3 heteroatoms. The heteroaryl group can include groups such as pyrrole, pyridine, imidazole, pyrazole, triazole, tetrazole, pyrazine, pyrimidine, pyridazine, triazine (1,2,3-, 1,2,4- and 1,3,5-isomers), thiophene, furan, thiazole, isothiazole, oxazole, and isoxazole. The heteroaryl groups can also be fused to aromatic ring systems, such as a phenyl ring, to form members including, but not limited to, benzopyrroles such as indole and isoindole, benzopyridines such as quinoline and isoquinoline, benzopyrazine (quinoxaline), benzopyrimidine (quinazoline), benzopyridazines such as phthalazine and cinnoline, benzothiophene, and benzofuran. Other heteroaryl groups include heteroaryl rings linked by a bond, such as bipyridine. Heteroaryl groups can be substituted or unsubstituted.
[0057] “Alkyl-heteroaryl” refers to a radical having an alkyl component and a heteroaryl component, where the alkyl component links the heteroaryl component to the point of attachment. The alkyl component is as defined above, except that the alkyl component is at least divalent, an alkylene, to link to the heteroaryl component and to the point of attachment. The alkyl component can include any number of carbons, such as Co-6, C1-2, C1-3, CM, C1-5, Ci-6, C2-3, C2-4, C2-5, C2-6, C3-4, C3-5, C3-6, C4-5, C4-6 and C5-6. In some instances, the alkyl component can be absent. The heteroaryl component is as defined within. Alkyl-heteroaryl groups can be substituted or unsubstituted.
[0058] “Salt” refers to acid or base salts of the compounds used in the methods of the present invention. Illustrative examples of pharmaceutically acceptable salts are mineral acid (hydrochloric acid, hydrobromic acid, phosphoric acid, and the like) salts, organic acid (fumaric acid, acetic acid, propionic acid, glutamic acid, citric acid and the like) salts, quaternary ammonium (methyl iodide, ethyl iodide, and the like) salts. It is understood that the pharmaceutically acceptable salts are non-toxic. Additional information on suitable pharmaceutically acceptable salts can be found in Remington's Pharmaceutical Sciences, 17th ed., Mack Publishing Company, Easton, Pa., 1985, which is incorporated herein by reference.
[0059] The neutral forms of the compounds may be regenerated by contacting the salt with a base or acid and isolating the parent compound in the conventional manner. The parent form of the compound differs from the various salt forms in certain physical properties, such as solubility in polar solvents, but otherwise the salts are equivalent to the parent form of the compound for the purposes of the present invention. [0060] “Pharmaceutically acceptable salt” refers to a compound in salt form, wherein the compound are suitable for administration to a subject. Representative pharmaceutically acceptable salts include salts of acetic, ascorbic, benzenesulfonic, benzoic, camphorsulfonic, citric, ethanesulfonic, edisylic, fumaric, gentisic, gluconic, glucoronic, glutamic, hippuric, hydrobromic, hydrochloric, isethionic, lactic, lactobionic, maleic, malic, mandelic, methanesulfonic, mucic, naphthalenesulfonic, naphthalene- 1,5-disulfonic, naphthalene-2, 6- disulfonic, nicotinic, nitric, orotic, pamoic, pantothenic, phosphoric, succinic, sulfuric, tartaric, p-toluenesulfonic and xinafoic acid, and the like
[0061] “Pharmaceutically acceptable excipient” refers to a substance that aids the administration of an active agent to and absorption by a subject. Pharmaceutical excipients useful in the present invention include, but are not limited to, binders, fillers, disintegrants, lubricants, coatings, sweeteners, flavors and colors. One of skill in the art will recognize that other pharmaceutical excipients are useful in the present invention.
[0062] “Composition” refers to a product comprising the specified ingredients in the specified amounts, as well as any product, which results, directly or indirectly, from combination of the specified ingredients in the specified amounts. By“pharmaceutically acceptable” it is meant the carrier, diluent or excipient must be compatible with the other ingredients of the formulation.
[0063] “Isomers” refers to compounds with same chemical formula but different connectivity between the atoms in the molecule, leading to distinct chemical structures. Isomers include structural isomers and stereoisomers. Examples of structural isomers include, but are not limited to tautomers and regioisomers. Examples of stereoisomers include but are not limited to diastereomers and enantiomers.
[0064] “Administering” refers to oral administration, administration as a suppository, topical contact, parenteral, intravenous, intraperitoneal, intramuscular, intralesional, intranasal or subcutaneous administration, intrathecal administration, or the implantation of a slow-release device e.g., a mini-osmotic pump, to the subject.
[0065] “Subject” refers to animals such as mammals, including, but not limited to, primates (e.g., humans), cows, sheep, goats, horses, dogs, cats, rabbits, rats, mice and the like. In certain embodiments, the subject is a human. [0066] “Therapeutically effective amount” or“therapeutically sufficient amount” or “effective or sufficient amount” refers to a dose that produces therapeutic effects for which it is administered. The exact dose will depend on the purpose of the treatment, and will be ascertainable by one skilled in the art using known techniques (see, e.g. , Lieberman, Pharmaceutical Dosage Forms (vols. 1-3, 1992); Lloyd, The Art, Science and Technology of Pharmaceutical Compounding (1999); Pickar, Dosage Calculations (1999); and Remington: The Science and Practice of Pharmacy, 20th Edition, 2003, Gennaro, Ed., Lippincott, Williams & Wilkins). In sensitized cells, the therapeutically effective dose can often be lower than the conventional therapeutically effective dose for non- sensitized cells.
[0067] “Neuronal plasticity” refers to the ability of the brain to change its structure and/or function continuously throughout a subject’s life. Examples of the changes to the brain include, but are not limited to, the ability to adapt or respond to internal and/or external stimuli, such as due to an injury, and the ability to produce new neurites, dendritic spines, and synapses.
[0068] “Brain disorder” refers to a neurological disorder which affects the brain’s structure and function. Brain disorders can include, but are not limited to, Alzheimer’s, Parkinson’s disease, psychological disorder, depression, treatment resistant depression, addiction, anxiety, post-traumatic stress disorder, suicidal ideation, major depressive disorder, bipolar disorder, schizophrenia, stroke, traumatic brain injury, and substance use disorder.
[0069] “Combination therapy” refers to a method of treating a disease or disorder, wherein two or more different pharmaceutical agents are administered in overlapping regimens so that the subject is simultaneously exposed to both agents. For example, the compounds of the invention can be used in combination with other pharmaceutically active compounds. The compounds of the invention can be administered simultaneously (as a single preparation or separate preparation) or sequentially to the other drug therapy. In general, a combination therapy envisions administration of two or more drugs during a single cycle or course of therapy.
[0070] “Neurotrophic factors” refers to a family of soluble peptides or proteins which support the survival, growth, and differentiation of developing and mature neurons.
[0071] “Modulate” or“modulating” or“modulation” refers to an increase or decrease in the amount, quality, or effect of a particular activity, function or molecule. By way of illustration and not limitation, agonists, partial agonists, antagonists, and allosteric modulators (e.g., a positive allosteric modulator) of a G protein-coupled receptor (e.g., 5HT2A) are modulators of the receptor.
[0072] “Agonism” refers to the activation of a receptor or enzyme by a modulator, or agonist, to produce a biological response.
[0073] “Agonist” refers to a modulator that binds to a receptor or enzyme and activates the receptor to produce a biological response. By way of example only,“5HT2A agonist” can be used to refer to a compound that exhibits an EC50 with respect to 5HT2A activity of no more than about 100 mM. In some embodiments,“agonist” includes full agonists or partial agonists. “Full agonist” refers to a modulator that binds to and activates a receptor with the maximum response that an agonist can elicit at the receptor.“Partial agonist” refers to a modulator that binds to and activates a given receptor, but has partial efficacy, that is, less than the maximal response, at the receptor relative to a full agonist.
[0074] “Positive allosteric modulator” refers to a modulator that binds to a site distinct from the orthosteric binding site and enhances or amplifies the effect of an agonist.
[0075] “Antagonism” refers to the inactivation of a receptor or enzyme by a modulator, or antagonist. Antagonism of a receptor, for example, is when a molecule binds to the receptor and does not allow activity to occur.
[0076] “Antagonist” or“neutral antagonist” refers to a modulator that binds to a receptor or enzyme and blocks a biological response. An antagonist has no activity in the absence of an agonist or inverse agonist but can block the activity of either, causing no change in the biological response.
[0077] “IC50” refers to the concentration of a substance (e.g., a compound or a drug) that is required for 50% inhibition of a biological process. For example, IC50 refers to the half maximal (50%) inhibitory concentration (IC) of a substance as determined in a suitable assay. In some instances, an IC50 is determined in an in vitro assay system. In some embodiments as used herein, IC50 refers to the concentration of a modulator (e.g., an antagonist or inhibitor) that is required for 50% inhibition of a receptor, for example, 5HT2A.
III. Compounds
[0078] The present invention provides azepino-indoles and other heterocyclic non- hallucinogenic compounds (e.g., Formula (I) or Formula (la)) useful for the treatment of a variety of brain disorders and other conditions. In some embodiments, azepino-indoles and other heterocyclic compounds provided herein are 5-HT2A modulators and promote neural plasticity (e.g., cortical structural plasticity).
[0079] In some embodiments, the present invention provides a compound of Formula I:
Figure imgf000022_0001
wherein: R1 is hydrogen or Ci-6 alkyl; R3a and R3b are each independently hydrogen, Ci-6 alkyl, Cvx cycloalkyl, or C4-14 alkyl-cycloalkyl; R3c is hydrogen or Ci-6 alkyl; alternatively,
R2 and R3b are combined with the atoms to which they are attached to form a C5- 8 heterocycloalkyl substituted with 1 to 3 R9 groups which are each independently hydrogen, Ci-6 alkyl, C vs cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl- heterocycloalkyl, Ce-i2 aryl, C7-18 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R9 groups attached to the same atom are combined to form =0; alternatively, R2 and R3c are combined with the atoms to which they are attached to form a Cvs cycloalkyl, substituted with 1 to 3 R10 groups which are each independently hydrogen, Ci-6 alkyl, C s cycloalkyl, C3- 14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-18 alkyl- aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R10 groups attached to the same atom are combined to form =0; R4, R5, R6 and R7 are each independently hydrogen, Ci-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, halogen, Ci-6 haloalkyl, Ci-6 alkylamine, Ci-6 alkoxy, Ci-6 haloalkoxy, - c), -
Figure imgf000022_0002
-8 cycloalkyl, C3- 14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, C6-12 aryl, C7-I8 alkyl- aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, wherein at least one of R4, R5, R6 and R7 is not H; alternatively, R4 and R5, R5 and R6, or R6 and R7 are combined with the atoms to which they are each attached to form a C3-6 cycloalkyl, C3-6 heterocycloalkyl, Ce-n aryl or C5-10 heteroaryl; R8a, R8b, R8c and R8d are each independently H, Ci-6 alkyl; wherein when R2 and R3b are combined with the atoms to which they are attached to form a C7 heterocycloalkyl, and R3a is methyl, then R5 is not OMe, OH, or Cl; wherein when R2 and R3b are combined with the atoms to which they are attached to form a C7 heterocycloalkyl, and R3a is ethyl, then R6 is not OMe; wherein when R2 and R3b are combined with the atoms to which they are attached to form a C7 heterocycloalkyl, and R6 is F, then at least one of R4, R5, and R7 is not hydrogen; wherein when R2 and R3b are combined with the atoms to which they are attached to form a C7 heterocycloalkyl, and R6 is F, Cl, -Me, or -OMe, then R3a is -Me; wherein when R2 and R3b are combined with the atoms to which they are attached to form a C6
heterocycloalkyl, and R6 is Ci-6 alkyl, halogen, Ci-6 alkyoxy, -C(0)FI, -NFI2, then at least one of R4, R5, and R7 is not hydrogen; and wherein when R2 and R3b are combined with the atoms to which they are attached to form a CV, heterocycloalkyl, and R6 is methyl, halogen, or - C(0)H, then at least one of R4, R5, and R7 is not methyl or Cl, or pharmaceutically acceptable salts and isomers thereof.
[0080] In some embodiments, the present invention provides a compound of Formula I
Figure imgf000023_0001
wherein R1 is hydrogen or Ci-6 alkyl; R3a and R3b are each independently hydrogen, Ci-6 alkyl, Cvx cycloalkyl, or C4-14 alkyl-cycloalkyl; R3c is hydrogen or Ci-6 alkyl; alternatively,
R2 and R3b are combined with the atoms to which they are attached to form a C5- 8 heterocycloalkyl substituted with 1 to 3 R9 groups which are each independently hydrogen, Ci-6 alkyl, C s cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl- heterocycloalkyl, Ce-i2 aryl, C7-18 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R9 groups attached to the same atom are combined to form =0; alternatively, R2 and R3b are combined with the atoms to which they are attached to form a Cvs cycloalkyl, substituted with 1 to 3 R10 groups which are each independently hydrogen, Ci-6 alkyl, C s cycloalkyl, C3- 14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-I8 alkyl- aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R10 groups attached to the same atom are combined to form =0; R4, R5, R6 and R7 are each independently hydrogen, Ci-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, halogen, Ci-6 haloalkyl, Ci-6 alkylamine, Ci-6 alkoxy, Ci-6 haloalkoxy, - c), -
Figure imgf000023_0002
-8 cycloalkyl, C3- 14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, C6-12 aryl, C7-18 alkyl- aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, wherein at least one of R4, R5, R6 and R7 is not H; R8b, R8c and R8d are each independently H or Ci-6 alkyl; or pharmaceutically acceptable salts and isomers thereof.
[0081] In some embodiments, R1 is hydrogen or Ci-6 alkyl. In some embodiments, R1 is hydrogen, methyl, ethyl, or propyl. In some embodiments, R1 is hydrogen or methyl. In some embodiments, R1 is hydrogen. In some embodiments, R1 is methyl. In some embodiments, the present invention provides a compound, or a pharmaceutically acceptable salt thereof, wherein R1 is hydrogen or methyl. In some embodiments, the present invention provides a compound, or a pharmaceutically acceptable salt thereof, wherein R1 is hydrogen.
[0082] In some embodiments, R3a is hydrogen, Ci-6 alkyl, CYx cycloalkyl, or C4-14 alkyl- cycloalkyl. In some embodiments, R3a is hydrogen or Ci-6 alkyl. In some embodiments, R3a is hydrogen, methyl, ethyl, or propyl. In some embodiments, R3a is hydrogen or methyl. In some embodiments R3a is methyl. In some embodiments, R3a is hydrogen. In some embodiments, the present invention provides a compound, or a pharmaceutically acceptable salt thereof, wherein R3a is hydrogen or Ci-6 alkyl. In some embodiments, the present invention provides a compound, or a pharmaceutically acceptable salt thereof, wherein R3a is hydrogen or methyl.
[0083] In some embodiments, R4, R5, R6 and R7 are each independently hydrogen, Ci-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, halogen, Ci-6 haloalkyl, Ci-6 alkylamine, Ci-6 alkoxy, Ci-6 haloalkoxy, -OR8a, -NO2, -CN, -C(0)R8b, -C(0)OR8b, -OC(0)R8b, -OC(0)OR8b, -N(R8bR8c),
Figure imgf000024_0001
cycloalkyl, C3- 14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, C6-12 aryl, C7-18 alkyl- aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, wherein at least one of R4, R5, R6 and R7 is not H; alternatively, R4 and R5, R5 and R6, or R6 and R7 are combined with the atoms to which they are each attached to form a C3-6 cycloalkyl, C3-6 heterocycloalkyl, Ce-n aryl or C5-10 heteroaryl; and R8a, R8b, R8c and R8d are each independently H, Ci-6 alkyl.
[0084] In some embodiments, the present invention provides a compound, or a
pharmaceutically acceptable salt thereof, wherein: R4, R5, R6 and R7 are each independently hydrogen, Ci-6 alkyl, halogen, Ci-6 haloalkyl, Ci-6 alkoxy, Ci-6 haloalkoxy, -OR8a, -NO2, - CN, -N(R8bR8c), -N(R8b)C(0)R8c, -C(0)N(R8bR8c), CYs cycloalkyl, C3-14 alkyl-cycloalkyl, C4- 10 heterocycloalkyl, or C4-16 alkyl-heterocycloalkyl, wherein at least one of R4, R5, R6 and R7 is not H; alternatively, R4 and R5, R5 and R6, or R6 and R7 are combined with the atoms to which they are each attached to form a C3-6 heterocycloalkyl; and R8a, R8b or R8c are each independently H, Ci-6 alkyl, or C3-6 cycloalkyl.
[0085] In some embodiments, the present invention provides a compound, or a
pharmaceutically acceptable salt thereof, wherein R6 is Ci-6 alkyl, halogen, Ci-6 haloalkyl, Ci- 6 alkoxy, Ci-b haloalkoxy, -OR8a, -NO2, -CN, -N(R8bR8c), -N(R8b)C(0)R8c, -C(0)N(R8bR8c), C3-8 cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, or C4-16 alkyl-heterocycloalkyl; alternatively, R5 and R6 are combined with the atoms to which they are each attached to form a C3-6 heterocycloalkyl; and R8a, R8b andR8c are each independently H, Ci-6 alkyl, or C3-6 cycloalkyl.
[0086] In some embodiments, the present invention provides a compound, or a
pharmaceutically acceptable salt thereof, wherein R4, R5, R6 and R7 are each independently hydrogen, Ci-6 alkyl, halogen, Ci-6 haloalkyl, Ci-6 alkoxy, Ci-6 haloalkoxy, -OR8a, - N(R8bR8c), -N(R8b)C(0)R8c, C4-10 heterocycloalkyl, or C4-16 alkyl-heterocycloalkyl, wherein at least one of R4, R5, R6 and R7 is not H; alternatively, R5 and R6 are combined with the atoms to which they are each attached to form a C3-6 heterocycloalkyl; and R8a, R8b and R8c are each independently H, Ci-6 alkyl, or C3-6 cycloalkyl.
[0087] In some embodiments, the present invention provides a compound, or a
pharmaceutically acceptable salt thereof, wherein: R6 is Ci-6 alkyl, halogen, Ci-6 haloalkyl, Ci-6 alkoxy, Ci-6 haloalkoxy, -OR8a, -N(R8bR8c), -N(R8b)C(0)R8c, C4-10 heterocycloalkyl, or C4-16 alkyl-heterocycloalkyl; alternatively, R5 and R6 are combined with the atoms to which they are each attached to form a C3-6 heterocycloalkyl; and R8a, R8b and R8c are each independently H, Ci-6 alkyl, or C3-6 cycloalkyl.
[0088] In some embodiments, the present invention provides a compound, or a
pharmaceutically acceptable salt thereof, wherein: R4, R5, R6 and R7 are each independently hydrogen, Ci-6 alkyl, halogen, Ci-6 haloalkyl, Ci-6 alkoxy, Ci-6 haloalkoxy, -OR8a, - N(R8bR8c), or -N(R8b)C(0)R8c wherein at least one of R4, R5, R6 and R7 is not H; R8a is H; and R8b and R8c are each independently H or -Me; alternatively, R5 and R6 are combined with the atoms to which they are each attached to form a C3-6 heterocycloalkyl.
[0089] In some embodiments, the present invention provides a compound, wherein R4, R5, R6 and R7 are each independently hydrogen, Ci-6 alkyl, halogen, Ci-6 haloalkyl, Ci-6 alkoxy, Ci-6 haloalkoxy, or -OR8a, wherein at least one of R4, R5, R6 and R7 is not H; and R8a is H; alternatively, R5 and R6 can be combined with the atoms to which they are each attached to form a C3-6 heterocycloalkyl.
[0090] In some embodiments, the present invention provides a compound, or a
pharmaceutically acceptable salt thereof, wherein: R4, R5, R6 and R7 are each independently H, F, Cl, Br, -OH, -OMe, -CF3 -OCF3, -Me, -NMe2, -NHC(0)Me, or -N(Me)C(0)Me, wherein at least one of R4, R5, R6 and R7 is not H; alternatively, R5 and R6 are combined to form a 1,3-dioxole ring, or 1,4-dioxane ring.
[0091] In some embodiments, the present invention provides a compound, or a
pharmaceutically acceptable salt thereof, wherein: R4 is H, F, -Me, -CF3, -OCF3, or -OMe; R5 is H, F, Cl, Br, -Me, -CF3, -OCF3, -OH or -OMe; R6 is H, F, -OH, -OMe, -OiPr, -Me, -CF3, - OCF3, -NMC2, -NHC(0)Me, or -N(Me)C(0)Me; alternatively, R5 and R6 are combined to form a 1,3-dioxole ring or 1,4-dioxane ring; and R7 is H, wherein at least one of R4, R5 and R6 is not H.
[0092] In some embodiments, the present invention provides a compound, wherein R4, R5, R6 and R7 are each independently H, F, Cl, Br, -OH, -OMe, -CF3 or -OCF3, wherein at least one of R4, R5, R6 and R7 is not H; alternatively, R5 and R6 are combined to form a 1,3-dioxole ring.
[0093] In some embodiments, the present invention provides a compound, wherein R4 is H, F, or -OMe; R5 is H, F, Cl, Br, -OH or -OMe; R6 is H, F, -OMe, -CF3 or -OCF3; alternatively, R5 and R6 are combined to form a 1,3-dioxole ring; and R7 is H, wherein at least one of R4, R5, R6 and R7 is not H.
[0094] In some embodiments, the present invention provides a compound, or a
pharmaceutically acceptable salt thereof, wherein R6 is F, -OH, -OMe, -OiPr, -Me, -CF3, - OCF3, -NMC2, -NHC(0)Me, or -N(Me)C(0)Me; alternatively, R5 and R6 are combined to form a 1,3-dioxole ring or 1,4-dioxane ring.
[0095] In some embodiments, R4 and R5, R5 and R6, or R6 and R7 are combined with the atoms to which they are each attached to form a C3-6 cycloalkyl, C3-6 heterocycloalkyl, C6-12 aryl or C5-10 heteroaryl. In some embodiments, R4 and R5, R5 and R6, or R6 and R7 are combined with the atoms to which they are each attached to form a C3-6 cycloalkyl or C3-6 heterocycloalkyl. In some embodiments, R4 and R5 are combined with the atoms to which they are each attached to form a C3-6 heterocycloalkyl. In some embodiments, R5 and R6 are combined with the atoms to which they are each attached to form a C3-6 heterocycloalkyl. In some embodiments, R6 and R7 are combined with the atoms to which they are each attached to form a C3-6 heterocycloalkyl. In some embodiments, R5 and R6 are combined to form a 1,3-dioxole ring or 1,4-dioxane ring.
[0096] In some embodiments, R3a and R3b are each independently hydrogen, Ci-6 alkyl, C3-8 cycloalkyl, or C4-14 alkyl-cycloalkyl; alternatively, R2 and R3b are combined with the atoms to which they are attached to form a C5-8 heterocycloalkyl substituted with 1 to 3 R9 groups which are each independently hydrogen, Ci-6 alkyl, CYx cycloalkyl, C3-14 alkyl-cycloalkyl, C4- 10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-18 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R9 groups attached to the same atom are combined to form =0; alternatively, R2 and R3c are combined with the atoms to which they are attached to form a C5-8 cycloalkyl, substituted with 1 to 3 R10 groups which are each independently hydrogen, Ci-6 alkyl, C3-8 cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl- heterocycloalkyl, Ce-n aryl, C7-18 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R10 groups attached to the same atom are combined to form =0.
[0097] In some embodiments, the present invention provides a compound of the present invention, or a pharmaceutically acceptable salt thereof, wherein R2 and R3b are combined with the atoms to which they are attached to form a C5-8 heterocycloalkyl substituted with 1 to 3 R9 groups which are each independently hydrogen, Ci-6 alkyl, C3-8 cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-I8 alkyl- aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R9 groups attached to the same atom are combined to form =0. In some embodiments, the present invention provides a compound, or a pharmaceutically acceptable salt thereof, wherein R2 and R3b are combined with the atoms to which they are attached to form a C5-8 heterocycloalkyl. In some embodiments, the present invention provides a compound, or a pharmaceutically acceptable salt thereof, wherein R2 and R3b are combined with the atoms to which they are attached to form a C7- 8 heterocycloalkyl.
[0098] In some embodiments, the present invention provides a compound, or a
pharmaceutically acceptable salt thereof, wherein the compound of Formula I has the following structure:
Figure imgf000028_0001
wherein: R6 is Ci-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, halogen, Ci-6 haloalkyl, Ci-6 alkylamine, Ci-6 alkoxy, Ci-b haloalkoxy, -OR8a, -NO2, -CN, -C(0)R8b, -C(0)0R8b, -0C(0)R8b, - 0C(0)0R8b, -N(R8bR8c), -N(R8b)C(0)R8c, -C(0)N(R8bR8c), -N(R8b)C(0)0R8c, - 0C(0)N(R8bR8c), -N(R8b)C(0)N(R8cR8d), -C(0)C(0)N(R8bR8c), -S(0 )R8b, -S(0) N(R8bR8c), C3-8 cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-18 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl; alternatively, R4 and R5, R5 and R6, or R6 and R7 are combined with the atoms to which they are each attached to form a C3-6 cycloalkyl, C3-6 heterocycloalkyl, Ce-n aryl or C5-10 heteroaryl; R8a, R8b, R8c and R8d are each independently H, Ci-6 alkyl; and n is 1, 2, or 3; wherein when n is 2, and R6 is F, then at least one of R4, R5, and R7 is not hydrogen; wherein when n is 2 and R6 is F, Cl, methyl, or OMe, then R3a is methyl; wherein when n is 1, and R6 is Ci-6 alkyl, halogen, Ci-6 alkyoxy, - C(0)H, -NH2, then at least one of R4, R5, and R7 is not hydrogen; and wherein when n is 1, and R6 is methyl, halogen, or -C(0)H, then at least one of R4, R5, and R7 is not methyl or Cl.
[0099] In some embodiments, the present invention provides a compound of Formula (la), or a pharmaceutically acceptable salt or isomer thereof, wherein the compound has the following structure:
Figure imgf000028_0002
wherein: R1 is hydrogen or Ci-6 alkyl; R3a is hydrogen, Ci-6 alkyl, C3-8 cycloalkyl, or C4-14 alkyl-cycloalkyl R3c is hydrogen or Ci-6 alkyl; R4, R5, and R7 are each independently hydrogen, Ci-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, halogen, Ci-6 haloalkyl, Ci-6 alkylamine, Ci-6 alkoxy, Ci-e haloalkoxy, -OR8a, -NO2, -CN, -C(0)R8b, -C(0)0R8b, -0C(0)R8b, -0C(0)0R8b, -N(R8bR8c), -N(R8b)C(0)R8c, -C(0)N(R8bR8c), -N(R8b)C(0)0R8c, -0C(0)N(R8bR8c), - N (R8b)C(0)N (R8cR8d), -C(0)C(0)N (R8bR8c), -S(0 )R8b, -S(0) N(R8bR8c), C3-8 cycloalkyl, C3- 14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, C6-12 aryl, C7-18 alkyl- aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl; R6 is Ci-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, halogen, Ci-6 haloalkyl, Ci-6 alkylamine, Ci-6 alkoxy, Ci-6 haloalkoxy, -OR8a, -NO2, -CN, - C(0)R8b, -C(0)OR8b, -OC(0)R8b, -OC(0)OR8b, -N(R8bR8c), -N(R8b)C(0)R8c, - C(0)N(R8bR8c), -N (R8b)C(0)0R8c, -0C(0)N(R8bR8c), -N(R8b)C(0)N(R8cR8d), - C(0)C(0)N (R8bR8c), -S(02)R8b, -S(0)2N(R8bR8c), CYx cycloalkyl, C3-14 alkyl-cycloalkyl, C - 10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-18 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl; alternatively, R4 and R5, R5 and R6, or R6 and R7, can be combined with the atoms to which they are each attached to form a CYs cycloalkyl or a C3-6
heterocycloalkyl; R8a, R8b, R8c, and R8d are each independently hydrogen, Ci-6 alkyl, or C3-6 cycloalkyl; n is 1, 2, or 3; or salts and isomers thereof; and wherein when n is 1 and R6 is Ci-6 alkyl, halo, C(0)H, NH2, or Ci-6 alkoxy, then at least one of R4, R5, and R7 is not hydrogen; wherein when n is 1 and R6 is methyl, halogen, or C(0)H, then at least one of R4, R5, or R7 is not methyl or Cl; wherein when n is 2 and R6 is F, Cl, methyl, or OMe, then R3a is methyl; and wherein when n is 2 and R6 is F, then at least one of R4, R5, and R7 is not hydrogen.
[0100] In some embodiments, the present invention provides a compound, or a
pharmaceutically acceptable salt thereof, wherein the compound of Formula I or la has the following structure:
Figure imgf000029_0001
[0101] In some embodiments, R3a is methyl, and R2 and R3b are combined with the atoms to which they are attached to form a CYx heterocycloalkyl substituted with 1 to 3 R9 groups which are each independently hydrogen, Ci-6 alkyl, Cvs cycloalkyl, C3-14 alkyl-cycloalkyl, C4- 10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-18 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R9 groups attached to the same atom are combined to form =0. In some embodiments, R3a is methyl, and R2 and R3b are combined with the atoms to which they are attached to form a Ce-i heterocycloalkyl.
[0102] In some embodiments, the present invention provides a compound, or a
pharmaceutically acceptable salt thereof, wherein R3a is methyl. In some embodiments, the present invention provides a compound, or a pharmaceutically acceptable salt thereof, wherein the compound of Formula I or la has the following structure:
Figure imgf000030_0001
[0103] In some embodiments, the present invention provides a compound, or a pharmaceutically acceptable salt thereof, wherein the compound of Formula I or la has the following structure:
Figure imgf000030_0002
wherein: R5 and R6 are each independently H, F, Cl, Br, I, -OH, -OMe, -OiPr, -Me, -CF3,- OCF3, -NMe2, -NHC(0)Me, or -N(Me)C(0)Me, wherein at least one of R5 and R6 is not H; alternatively, R5 and R6 are combined to form a 1,3-dioxole ring or 1,4-dioxane ring.
[0104] In some embodiments, the present invention provides a compound, or a pharmaceutically acceptable salt thereof, wherein the compound of Formula I or la has the following structure:
Figure imgf000030_0003
wherein: R5 and R6 are each independently F, Cl, Br, I, -OH, -OMe, -OiPr, -Me, -CF3,-OCF3, -NMe2, -NHC(0)Me, or -N(Me)C(0)Me; alternatively, R5 and R6 are combined to form a 1,3-dioxole ring or 1,4-dioxane ring.
[0105] In some embodiments, the present invention provides a compound, wherein the compound of Formula I or la has the following structure:
Figure imgf000030_0004
wherein R5 is H, F, Cl, Br, -OH or -OMe; and R6 is H, F, -OMe, -CF3 or -OCF3, wherein at least one of R5 and R6 is not H. [0106] In some embodiments, the present invention provides a compound, or a
pharmaceutically acceptable salt thereof, wherein the compound of Formula I or la has the following structure:
Figure imgf000031_0001
wherein: R4 and R6 are each independently H, F, Cl, Br, I, -OH, -OMe, -OiPr, -Me, -CF3,- OCF3, -NMe2, -NHC(0)Me, or -N(Me)C(0)Me, wherein at least one of R4 and R6 is not H.
[0107] In some embodiments, the present invention provides a compound, or a
pharmaceutically acceptable salt thereof, wherein the compound of Formula I or la has the following structure:
Figure imgf000031_0002
wherein: R4 and R6 are each independently F, Cl, Br, I, -OH, -OMe, -OiPr, -Me, -CF3,-OCF3, -NMe2, -NHC(0)Me, or -N(Me)C(0)Me.
[0108] In some embodiments, the present invention provides a compound, wherein the compound is:
Figure imgf000031_0003
Figure imgf000032_0001
or a pharmaceutically acceptable salt thereof.
[0109] In some embodiments, the present invention provides a compound, or a pharmaceutically acceptable salt thereof, wherein the compound is
Figure imgf000032_0002
[0110] In some embodiments, the present invention provides a compound, or a pharmaceutically acceptable salt thereof, wherein the compound is
Figure imgf000033_0001
[0111] In some embodiments, the present invention provides a compound, or a 5 pharmaceutically acceptable salt thereof, wherein the compound is
Figure imgf000033_0002
Figure imgf000034_0001
[0112] In some embodiments, the present invention provides a compound, or a
pharmaceutically acceptable salt thereof, wherein the compound is
Figure imgf000034_0002
[0113] In some embodiments, the present invention provides a compound, or a
pharmaceutically acceptable salt thereof, wherein the compound is
Figure imgf000034_0003
[0114] In some embodiments, R3a is hydrogen, and R2 and R3b are co with the atoms to which they are attached to form a C x heterocycloalkyl substituted with 1 to 3 R9 groups which are each independently hydrogen, Ci-6 alkyl, Cvs cycloalkyl, C3-14 alkyl-cycloalkyl, C4- 10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-18 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R9 groups attached to the same atom are combined to form =0. In some embodiments, R3a is hydrogen, and R2 and R3b are combined with the atoms to which they are attached to form a C heterocycloalkyl.
[0115] In some embodiments, the present invention provides a compound, or a pharmaceutically acceptable salt thereof, wherein the compound of Formula I or la has the following structure:
Figure imgf000035_0001
[0116] In some embodiments, the present invention provides a compound, or a pharmaceutically acceptable salt thereof, wherein the compound is
Figure imgf000035_0002
[0117] In some embodiments, R3c is hydrogen or Ci-6 alkyl. In some embodiments, R3c is hydrogen, methyl, ethyl, or propyl. In some embodiments R3c is hydrogen.
[0118] In some embodiments, R1 is Ci-6 alkyl. In some embodiments, R1 is methyl, ethyl, or propyl. In some embodiments, the present invention provides a compound, or a pharmaceutically acceptable salt thereof, wherein the compound of Formula I or la has the following structure:
Figure imgf000035_0003
[0119] In some embodiments, the present invention provides a compound, or a pharmaceutically acceptable salt thereof, wherein the compound is
Figure imgf000035_0004
[0120] In some embodiments, the present invention provides a compound, or a
pharmaceutically acceptable salt thereof, wherein R2 and R3c are combined with the atoms to which they are attached to form a C5-8 cycloalkyl, substituted with 1 to 3 R10 groups which are each independently hydrogen, Ci-6 alkyl, C3-8 cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-I8 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R10 groups attached to the same atom are combined to form =0.
[0121] In some embodiments, the present invention provides a compound, or a
pharmaceutically acceptable salt thereof, wherein R2 and R3c are combined with the atoms to which they are attached to form a C5-6 cycloalkyl, substituted with 1 to 2 R10 groups which are each hydrogen, or two R10 groups attached to the same atom are combined to form =0.
[0122] In some embodiments, the present invention provides a compound, or a
pharmaceutically acceptable salt thereof, wherein the compound is
Figure imgf000036_0001
[0123] The compounds of the present invention can also be in the salt forms, such as acid or base salts of the compounds of the present invention. Illustrative examples of
pharmaceutically acceptable salts are mineral acid (hydrochloric acid, hydrobromic acid, phosphoric acid, and the like) salts, organic acid (fumaric acid, acetic acid, propionic acid, glutamic acid, citric acid and the like) salts, quaternary ammonium (methyl iodide, ethyl iodide, and the like) salts. It is understood that the pharmaceutically acceptable salts are non toxic. Additional information on suitable pharmaceutically acceptable salts can be found in Remington's Pharmaceutical Sciences, 17th ed., Mack Publishing Company, Easton, Pa., 1985, which is incorporated herein by reference.
[0124] The present invention also includes isotopically-labeled compounds of the present invention, wherein one or more atoms are replaced by one or more atoms having specific atomic mass or mass numbers. Examples of isotopes that can be incorporated into compounds of the invention include, but are not limited to, isotopes of hydrogen, carbon, nitrogen, oxygen, fluorine, sulfur, and chlorine (such as 2H, 3H, 13C, 14C, 15N, 180, 170, 18F,
35S and 36C1). Isotopically-labeled compounds of the present invention are useful in assays of the tissue distribution of the compounds and their prodrugs and metabolites; preferred isotopes for such assays include 3H and 14C. In addition, in certain circumstances substitution with heavier isotopes, such as deuterium (2H), can provide increased metabolic stability, which offers therapeutic advantages such as increased in vivo half-life or reduced dosage requirements. Isotopically-labeled compounds of this invention can generally be prepared according to the methods known by one of skill in the art by substituting an isotopically- labeled reagent for a non-isotopically labeled reagent. Compounds of the present invention can be isotopically labeled at positions adjacent to the basic amine, in aromatic rings, and the methyl groups of methoxy substituents.
[0125] The present invention includes all tautomers and stereoisomers of compounds of the present invention, either in admixture or in pure or substantially pure form. The compounds of the present invention can have asymmetric centers at the carbon atoms, and therefore the compounds of the present invention can exist in diastereomeric or enantiomeric forms or mixtures thereof. All conformational isomers (e.g., cis and trans isomers) and all optical isomers (e.g., enantiomers and diastereomers), racemic, diastereomeric and other mixtures of such isomers, as well as solvates, hydrates, isomorphs, polymorphs and tautomers are within the scope of the present invention. Compounds according to the present invention can be prepared using diastereomers, enantiomers or racemic mixtures as starting materials.
Furthermore, diastereomer and enantiomer products can be separated by chromatography, fractional crystallization or other methods known to those of skill in the art.
IV. Pharmaceutical Compositions and Formulations
[0126] In some embodiments, the present invention provides a pharmaceutical composition comprising a compound of the present invention, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient.
[0127] The pharmaceutical compositions of the present invention can be prepared in a wide variety of oral, parenteral and topical dosage forms. Oral preparations include tablets, pills, powder, capsules, liquids, lozenges, cachets, gels, syrups, slurries, suspensions, etc., suitable for ingestion by the patient. The compositions of the present invention can also be administered by injection, that is, intravenously, intramuscularly, intracutaneously, subcutaneously, intraduodenally, or intraperitoneally. Also, the compositions described herein can be administered by inhalation, for example, intranasally. Additionally, the compositions of the present invention can be administered transdermally. The compositions of this invention can also be administered by intraocular, intravaginal, and intrarectal routes including suppositories, insufflation, powders and aerosol formulations (for examples of steroid inhalants, see Rohatagi, J. Clin. Pharmacol. 35: 1187-1193, 1995; Tjwa, Ann. Allergy Asthma Immunol. 75: 107-111, 1995). Accordingly, the present invention also provides pharmaceutical compositions including a pharmaceutically acceptable carrier or excipient and the compounds of the present invention.
[0128] For preparing pharmaceutical compositions from the compounds of the present invention, pharmaceutically acceptable carriers can be either solid or liquid. Solid form preparations include powders, tablets, pills, capsules, cachets, suppositories, and dispersible granules. A solid carrier can be one or more substances, which may also act as diluents, flavoring agents, binders, preservatives, tablet disintegrating agents, or an encapsulating material. Details on techniques for formulation and administration are well described in the scientific and patent literature, see, e.g., the latest edition of Remington's Pharmaceutical Sciences, Maack Publishing Co, Easton PA ("Remington's").
[0129] In powders, the carrier is a finely divided solid, which is in a mixture with the finely divided active component. In tablets, the active component is mixed with the carrier having the necessary binding properties in suitable proportions and compacted in the shape and size desired. The powders and tablets preferably contain from 5% to 70% or 10% to 70% of the compounds of the present invention.
[0130] Suitable solid excipients include, but are not limited to, magnesium carbonate; magnesium stearate; talc; pectin; dextrin; starch; tragacanth; a low melting wax; cocoa butter; carbohydrates; sugars including, but not limited to, lactose, sucrose, mannitol, or sorbitol, starch from com, wheat, rice, potato, or other plants; cellulose such as methyl cellulose, hydroxypropylmethyl-cellulose, or sodium carboxymethylcellulose; and gums including arabic and tragacanth; as well as proteins including, but not limited to, gelatin and collagen.
If desired, disintegrating or solubilizing agents may be added, such as the cross-linked polyvinyl pyrrolidone, agar, alginic acid, or a salt thereof, such as sodium alginate.
[0131] For preparing suppositories, a low melting wax, such as a mixture of fatty acid glycerides or cocoa butter, is first melted and the compounds of the present invention are dispersed homogeneously therein, as by stirring. The molten homogeneous mixture is then poured into convenient sized molds, allowed to cool, and thereby to solidify. [0132] Liquid form preparations include solutions, suspensions, and emulsions, for example, water or water/propylene glycol solutions. For parenteral injection, liquid preparations can be formulated in solution in aqueous polyethylene glycol solution.
[0133] Aqueous solutions suitable for oral use can be prepared by dissolving the compounds of the present invention in water and adding suitable colorants, flavors, stabilizers, and thickening agents as desired. Aqueous suspensions suitable for oral use can be made by dispersing the finely divided active component in water with viscous material, such as natural or synthetic gums, resins, methylcellulose, sodium carboxymethylcellulose, hydroxypropylmethylcellulose, sodium alginate, polyvinylpyrrolidone, gum tragacanth and gum acacia, and dispersing or wetting agents such as a naturally occurring phosphatide (e.g., lecithin), a condensation product of an alkylene oxide with a fatty acid (e.g., polyoxyethylene stearate), a condensation product of ethylene oxide with a long chain aliphatic alcohol (e.g., heptadecaethylene oxycetanol), a condensation product of ethylene oxide with a partial ester derived from a fatty acid and a hexitol (e.g., polyoxyethylene sorbitol mono-oleate), or a condensation product of ethylene oxide with a partial ester derived from fatty acid and a hexitol anhydride (e.g., polyoxyethylene sorbitan mono-oleate). The aqueous suspension can also contain one or more preservatives such as ethyl or n-propyl p-hydroxybenzoate, one or more coloring agents, one or more flavoring agents and one or more sweetening agents, such as sucrose, aspartame or saccharin. Formulations can be adjusted for osmolarity.
[0134] Also included are solid form preparations, which are intended to be converted, shortly before use, to liquid form preparations for oral administration. Such liquid forms include solutions, suspensions, and emulsions. These preparations may contain, in addition to the active component, colorants, flavors, stabilizers, buffers, artificial and natural sweeteners, dispersants, thickeners, solubilizing agents, and the like.
[0135] Oil suspensions can be formulated by suspending the compound of the present invention in a vegetable oil, such as arachis oil, olive oil, sesame oil or coconut oil, or in a mineral oil such as liquid paraffin; or a mixture of these. The oil suspensions can contain a thickening agent, such as beeswax, hard paraffin or cetyl alcohol. Sweetening agents can be added to provide a palatable oral preparation, such as glycerol, sorbitol or sucrose. These formulations can be preserved by the addition of an antioxidant such as ascorbic acid. As an example of an injectable oil vehicle, see Minto, J. Pharmacol. Exp. Ther. 281 :93-102, 1997. The pharmaceutical formulations of the invention can also be in the form of oil-in-water emulsions. The oily phase can be a vegetable oil or a mineral oil, described above, or a mixture of these. Suitable emulsifying agents include naturally-occurring gums, such as gum acacia and gum tragacanth, naturally occurring phosphatides, such as soybean lecithin, esters or partial esters derived from fatty acids and hexitol anhydrides, such as sorbitan mono- oleate, and condensation products of these partial esters with ethylene oxide, such as polyoxyethylene sorbitan mono-oleate. The emulsion can also contain sweetening agents and flavoring agents, as in the formulation of syrups and elixirs. Such formulations can also contain a demulcent, a preservative, or a coloring agent.
[0136] The compositions of the present invention can also be delivered as microspheres for slow release in the body. For example, microspheres can be formulated for administration via intradermal injection of drug- containing microspheres, which slowly release
subcutaneously (see Rao, J. Biomater Sci. Polym. Ed. 7:623-645, 1995; as biodegradable and injectable gel formulations (see, e.g., Gao Pharm. Res. 12:857-863, 1995); or, as
microspheres for oral administration (see, e.g., Eyles, J. Pharm. Pharmacol. 49:669-674, 1997). Both transdermal and intradermal routes afford constant delivery for weeks or months.
[0137] In some embodiments, the pharmaceutical compositions of the present invention can be formulated for parenteral administration, such as intravenous (IV) administration or administration into a body cavity or lumen of an organ. The formulations for administration will commonly comprise a solution of the compositions of the present invention dissolved in a pharmaceutically acceptable carrier. Among the acceptable vehicles and solvents that can be employed are water and Ringer's solution, an isotonic sodium chloride. In addition, sterile fixed oils can conventionally be employed as a solvent or suspending medium. For this purpose, any bland fixed oil can be employed including synthetic mono- or diglycerides. In addition, fatty acids such as oleic acid can likewise be used in the preparation of injectables. These solutions are sterile and generally free of undesirable matter. These formulations may be sterilized by conventional, well known sterilization techniques. The formulations may contain pharmaceutically acceptable auxiliary substances as required to approximate physiological conditions such as pFI adjusting and buffering agents, toxicity adjusting agents, e.g., sodium acetate, sodium chloride, potassium chloride, calcium chloride, sodium lactate and the like. The concentration of the compositions of the present invention in these formulations can vary widely, and will be selected primarily based on fluid volumes, viscosities, body weight, and the like, in accordance with the particular mode of administration selected and the patient's needs. For IV administration, the formulation can be a sterile injectable preparation, such as a sterile injectable aqueous or oleaginous suspension. This suspension can be formulated according to the known art using those suitable dispersing or wetting agents and suspending agents. The sterile injectable preparation can also be a sterile injectable solution or suspension in a nontoxic parenterally-acceptable diluent or solvent, such as a solution of 1,3-butanediol.
[0138] In some embodiments, the formulations of the compositions of the present invention can be delivered by the use of liposomes which fuse with the cellular membrane or are endocytosed, i.e., by employing ligands attached to the liposome, or attached directly to the oligonucleotide, that bind to surface membrane protein receptors of the cell resulting in endocytosis. By using liposomes, particularly where the liposome surface carries ligands specific for target cells, or are otherwise preferentially directed to a specific organ, one can focus the delivery of the compositions of the present invention into the target cells in vivo. (See, e.g., Al-Muhammed, J. Microencapsul. 13:293-306, 1996; Chonn, Curr. Opin.
Biotechnol. 6:698-708, 1995; Ostro, Am. J. Hosp. Pharm. 46: 1576-1587, 1989).
V. Administration
[0139] The compositions of the present invention can be delivered by any suitable means, including oral, parenteral and topical methods. Transdermal administration methods, by a topical route, can be formulated as applicator sticks, solutions, suspensions, emulsions, gels, creams, ointments, pastes, jellies, paints, powders, and aerosols.
[0140] The pharmaceutical preparation is preferably in unit dosage form. In such form the preparation is subdivided into unit doses containing appropriate quantities of the compounds of the present invention. The unit dosage form can be a packaged preparation, the package containing discrete quantities of preparation, such as packeted tablets, capsules, and powders in vials or ampoules. Also, the unit dosage form can be a capsule, tablet, cachet, or lozenge itself, or it can be the appropriate number of any of these in packaged form.
[0141] The compound of the present invention can be present in any suitable amount, and can depend on various factors including, but not limited to, weight and age of the subject, state of the disease, etc. Suitable dosage ranges for the compound of the present invention include from about 0.1 mg to about 10,000 mg, or about 1 mg to about 1000 mg, or about 10 mg to about 750 mg, or about 25 mg to about 500 mg, or about 50 mg to about 250 mg. Suitable dosages for the compound of the present invention include about 1 mg, 5, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 200, 300, 400, 500, 600, 700, 800, 900 or 1000 mg.
[0142] The compounds of the present invention can be administered at any suitable frequency, interval and duration. For example, the compound of the present invention can be administered once an hour, or two, three or more times an hour, once a day, or two, three, or more times per day, or once every 2, 3, 4, 5, 6, or 7 days, so as to provide the preferred dosage level. When the compound of the present invention is administered more than once a day, representative intervals include 5, 10, 15, 20, 30, 45 and 60 minutes, as well as 1, 2, 4,
6, 8, 10, 12, 16, 20, and 24 hours. The compound of the present invention can be administered once, twice, or three or more times, for an hour, for 1 to 6 hours, for 1 to 12 hours, for 1 to 24 hours, for 6 to 12 hours, for 12 to 24 hours, for a single day, for 1 to 7 days, for a single week, for 1 to 4 weeks, for a month, for 1 to 12 months, for a year or more, or even indefinitely.
[0143] The composition can also contain other compatible therapeutic agents. The compounds described herein can be used in combination with one another, with other active agents known to be useful in modulating a glucocorticoid receptor, or with adjunctive agents that may not be effective alone, but may contribute to the efficacy of the active agent.
[0144] The compounds of the present invention can be co-administered with another active agent. Co-administration includes administering the compound of the present invention and active agent within 0.5, 1, 2, 4, 6, 8, 10, 12, 16, 20, or 24 hours of each other. Co administration also includes administering the compound of the present invention and active agent simultaneously, approximately simultaneously (e.g., within about 1, 5, 10, 15, 20, or 30 minutes of each other), or sequentially in any order. Moreover, the compound of the present invention and the active agent can each be administered once a day, or two, three, or more times per day so as to provide the preferred dosage level per day.
[0145] In some embodiments, co-administration can be accomplished by co-formulation, i.e., preparing a single pharmaceutical composition including both the compound of the present invention and the active agent. In other embodiments, the compound of the present invention and the active agent can be formulated separately.
[0146] The compound of the present invention and the active agent can be present in the compositions of the present invention in any suitable weight ratio, such as from about 1 :100 to about 100: 1 (w/w), or about 1 :50 to about 50: 1 , or about 1 :25 to about 25: 1 , or about 1 :10 to about 10:1, or about 1 :5 to about 5:1 (w/w). The compound of the present invention and the other active agent can be present in any suitable weight ratio, such as about 1: 100 (w/w), 1 :50, 1 :25, 1 : 10, 1:5, 1 :4, 1 :3, 1:2, 1 : 1, 2: 1, 3:1, 4: 1, 5: 1, 10:1, 25: 1, 50: 1 or 100: 1 (w/w). Other dosages and dosage ratios of the compound of the present invention and the active agent are suitable in the compositions and methods of the present invention.
VI. Methods of Treatment
[0147] The compounds of the present invention can be used for increasing neuronal plasticity. The compounds of the present invention can also be used to treat any brain disease. The compounds of the present invention can also be used for increasing at least one of translation, transcription or secretion of neurotrophic factors.
[0148] In some embodiments, a compound of the present invention is used to treat neurological diseases. In some embodiments, the compounds have, for example, anti- addictive properties, antidepressant properties, anxiolytic properties, or a combination thereof. In some embodiments, the neurological disease is a neuropsychiatric disease. In some embodiments, the neuropsychiatric disease is a mood or anxiety disorder. In some embodiments, the neurological disease is a migraine, headaches (e.g., cluster headache), post- traumatic stress disorder (PTSD), anxiety, depression, neurodegenerative disorder,
Alzheimer’s disease, Parkinson’s disease, psychological disorder, treatment resistant depression, suicidal ideation, major depressive disorder, bipolar disorder, schizophrenia, stroke, traumatic brain injury, and addiction (e.g., substance use disorder). In some embodiments, the neurological disease is a migraine or cluster headache. In some embodiments, the neurological disease is a neurodegenerative disorder, Alzheimer’s disease, or Parkinson’s disease. In some embodiments, the neurological disease is a psychological disorder, treatment resistant depression, suicidal ideation, major depressive disorder, bipolar disorder, schizophrenia, post-traumatic stress disorder (PTSD), addiction (e.g., substance use disorder), depression, or anxiety. In some embodiments, the neuropsychiatric disease is a psychological disorder, treatment resistant depression, suicidal ideation, major depressive disorder, bipolar disorder, schizophrenia, post-traumatic stress disorder (PTSD), addiction (e.g., substance use disorder), depression, or anxiety. In some embodiments, the
neuropsychiatric disease or neurological disease is post-traumatic stress disorder (PTSD), addiction (e.g., substance use disorder), schizophrenia, depression, or anxiety. In some embodiments, the neuropsychiatric disease or neurological disease is addiction (e.g., substance use disorder). In some embodiments, the neuropsychiatric disease or neurological disease is depression. In some embodiments, the neuropsychiatric disease or neurological disease is anxiety. In some embodiments, the neuropsychiatric disease or neurological disease is post-traumatic stress disorder (PTSD). In some embodiments, the neurological disease is stroke or traumatic brain injury. In some embodiments, the neuropsychiatric disease or neurological disease is schizophrenia.
[0149] In some embodiments, a compound of the present invention is used for increasing neuronal plasticity. In some embodiments, the compounds described herein are used for treating a brain disorder. In some embodiments, the compounds described herein are used for increasing at least one of translation, transcription, or secretion of neurotrophic factors.
[0150] In some embodiments, the compounds of the present invention have activity as 5- HT2A modulators. In some embodiments, the compounds of the present invention have activity as 5-HT2A modulators. In some embodiments, the compounds of the present invention elicit a biological response by activating the 5-HT2A receptor (e.g., allosteric modulation or modulation of a biological target that activates the 5-HT2A receptor). 5-HT2A agonism has been correlated with the promotion of neural plasticity (Ly et al., 2018).
Hallucinogens (e.g., LSD and 5-MeO-DMT) activate a 5HT2A sensor assay in agonist mode, but their non-hallucinogenic congeners (lisuride (LIS) and 6-MeO-DMT) do not (FIG. 15). Moreover, compounds, such as, for example, 5-MeO-DMT, LSD, DMT, DOI, which are hallucinogenic in animals (e.g., humans), activate the 5HT2A sensor assay in agonist mode, whereas compounds, such as, for example, 6-MeO-DMT, LIS, 6-F-DET, L-MDMA, R- MDMA, Ketanserin, BOL148, which are non-hallucinogenic in animals (e.g., humans), do not activate the 5HT2A sensor assay in agonist mode (FIG. 16, at 10 mM of compound). In some embodiments, hallucinogenic potential of a compound of the present invention is determined in vitro. In some embodiments, hallucinogenic potential of a compound of the present invention is determined using a 5HT2A sensor assay. In some embodiments, the 5HT2A sensor assay is in an agonist mode or an antagonist mode. In some embodiments, the 5HT2A sensor assay is in an agonist mode. In some embodiments, a compound of the present invention that does not activate the sensor in agonist mode has non-hallucinogenic potential. In some embodiments, a compound of the present invention that does not activate the sensor in agonist mode is a non-hallucinogenic compound. [0151] Furthermore, non-hallucinogenic compounds (e.g., lisuride and 6-MeO-DMT) compete off 5-HT when the 5HT2A sensor assay is run in antagonist mode (FIG. 17A and FIG. 17B). Additionally, compounds, such as, for example, 6-F-DET, Ketanserin, BOL148, which are non-hallucinogenic in animals (e.g., humans), compete with 5HT binding to 5HT2A in the antagonist mode sensor assay (FIG. 18, at 10 mM of compound). In some
embodiments, a compound of the present invention that prevents binding of 5-HT to 5HT2A- In some embodiments, the 5HT2A sensor assay is in an antagonist mode. In some
embodiments, a compound of the present invention that prevents binding of 5-HT to 5HT2A and has non-hallucinogenic potential. In some embodiments, a compound of the present invention that prevents binding of 5-HT to 5HT2A and is non-hallucinogenic. In some embodiments, a compound of the present invention that prevents binding of 5-HT to 5HT2A in antagonist mode has non-hallucinogenic potential. In some embodiments, a compound of the present invention that prevents binding of 5-HT in antagonist mode is a non- hallucinogenic compound. In some embodiments, a compound of the present invention that inhibits the response of the sensor assay in antagonist mode has non-hallucinogenic potential. In some embodiments, a compound of the present invention that inhibits the response of the sensor assay in antagonist mode is a non-hallucinogenic compound.
[0152] In some embodiments, the results for the agonist mode sensor assay suggests a compound of the present invention is a non-hallucinogenic ligand of the 5-HT2A receptor. In some embodiments, the results for the antagonist mode sensor assay suggests a compound of the present invention is a non-hallucinogenic ligand of the 5-HT2A receptor. In some embodiments, the results for the agonist mode and antagonist mode sensor assay together suggest a compound of the present invention is a non-hallucinogenic ligand of the 5-HT2A receptor.
[0153] In some embodiments, the compounds described herein are selective 5-HT2A modulators. In some embodiments, the compounds described herein are 5-HT2A modulators and promote neural plasticity (e.g., cortical structural plasticity). In some embodiments, the compounds described herein are selective 5-HT2A modulators and promote neural plasticity (e.g., cortical structural plasticity). In some embodiments, promotion of neural plasticity includes, for example, increased dendritic spine growth, increased synthesis of synaptic proteins, strengthened synaptic responses, increased dendritic arbor complexity, increased dendritic branch content, increased spinogenesis, increased neuritogenesis, or any combination thereof. In some embodiments, increased neural plasticity includes, for example, increased cortical structural plasticity in the anterior parts of the brain.
[0154] In some embodiments, the 5-HT2A modulators (e.g., 5-HT2A agonists) are non- hallucinogenic. In some embodiments, non-hallucinogenic 5-HT2A modulators (e.g., 5-HT2A agonists) are used to treat neurological diseases, which modulators do not elicit dissociative side-effects. In some embodiments, the hallucinogenic potential of the compounds described herein is assessed in vitro. In some embodiments, the hallucinogenic potential assessed in vitro of the compounds described herein is compared to the hallucinogenic potential assessed in vitro of hallucinogenic homologs. In some embodiments, the compounds described herein elicit less hallucinogenic potential in vitro than the hallucinogenic homologs.
[0155] In some embodiments, non-hallucinogenic 5-HT2A modulators (e.g., 5-HT2A agonists) are used to treat neurological diseases. In some embodiments, the neurological diseases comprise decreased neural plasticity, decreased cortical structural plasticity, decreased 5-HT2A receptor content, decreased dendritic arbor complexity, loss of dendritic spines, decreased dendritic branch content, decreased spinogenesis, decreased neuritogenesis, retraction of neurites, or any combination thereof.
[0156] In some embodiments, non-hallucinogenic 5-HT2A modulators (e.g., 5-HT2A agonists) are used for increasing neuronal plasticity. In some embodiments, non- hallucinogenic 5-HT2A modulators (e.g., 5-HT2A agonists) are used for treating a brain disorder. In some embodiments, non-hallucinogenic 5-HT2A modulators (e.g., 5-HT2A agonists) are used for increasing at least one of translation, transcription, or secretion of neurotrophic factors.
A. Methods for Increasing Neuronal Plasticity
[0157] Neuronal plasticity refers to the ability of the brain to change structure and/or function throughout a subject’s life. New neurons can be produced and integrated into the central nervous system throughout the subject’s life. Increasing neuronal plasticity includes, but is not limited to, promoting neuronal growth, promoting neuritogenesis, promoting synaptogenesis, promoting dendritogenesis, increasing dendritic arbor complexity, increasing dendritic spine density, and increasing excitatory synapsis in the brain. In some
embodiments, increasing neuronal plasticity comprises promoting neuronal growth, promoting neuritogenesis, promoting synaptogenesis, promoting dendritogenesis, increasing dendritic arbor complexity, and increasing dendritic spine density.
[0158] In some embodiments, increasing neuronal plasticity can treat neurodegenerative disorder, Alzheimer’s, Parkinson’s disease, psychological disorder, depression, addiction, anxiety, post-traumatic stress disorder, treatment resistant depression, suicidal ideation, major depressive disorder, bipolar disorder, schizophrenia, stroke, traumatic brain injury, or substance use disorder.
[0159] In some embodiments, the present invention provides methods for increasing neuronal plasticity, comprising contacting a neuronal cell with any of the compounds of the present invention. In some embodiments, increasing neuronal plasticity improves a brain disorder described herein.
[0160] In some embodiments, a compound of the present invention is used to increase neuronal plasticity. In some embodiments, the compounds used to increase neuronal plasticity have, for example, anti- addictive properties, antidepressant properties, anxiolytic properties, or a combination thereof. In some embodiments, decreased neuronal plasticity is associated with a neuropsychiatric disease. In some embodiments, the neuropsychiatric disease is a mood or anxiety disorder. In some embodiments, the neuropsychiatric disease includes, for example, migraine, cluster headache, post-traumatic stress disorder (PTSD), schizophrenia, anxiety, depression, and addiction (e.g., substance abuse disorder). In some embodiments, brain disorders include, for example, migraines, addiction (e.g., substance use disorder), depression, and anxiety.
[0161] In some embodiments, the experiment or assay to determine increased neuronal plasticity of any compound of the present invention is a phenotypic assay, a dendritogenesis assay, a spinogenesis assay, a synaptogenesis assay, a Sholl analysis, a concentration- response experiment, a 5-HT2A agonist assay, a 5-HT2A antagonist assay, a 5-HT2A binding assay, or a 5-HT2A blocking experiment (e.g., ketanserin blocking experiments). In some embodiments, the experiment or assay to determine the hallucinogenic potential of any compound of Formula I or Formula (la) is a mouse head-twitch response (HTR) assay.
[0162] In some embodiments, the present invention provides a method for increasing neuronal plasticity, comprising contacting a neuronal cell with a compound of Formula I:
Figure imgf000048_0001
or a pharmaceutically acceptable salt thereof, in an amount sufficient to increase neuronal plasticity of the neuronal cell, wherein: R1 is hydrogen or Ci-6 alkyl; R3a and R3b are each independently hydrogen, Ci-6 alkyl, Cvs cycloalkyl, or C4-14 alkyl-cycloalkyl; R3c is hydrogen or Ci-6 alkyl; alternatively, R2 and R3b are combined with the atoms to which they are attached to form a C5-8 heterocycloalkyl substituted with 1 to 3 R9 groups which are each independently hydrogen, Ci-6 alkyl, C3-8 cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10
heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-I8 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R9 groups attached to the same atom are combined to form =0; alternatively, R2 and R3c are combined with the atoms to which they are attached to form a C5-8 cycloalkyl, substituted with 1 to 3 R10 groups which are each independently hydrogen, Ci-6 alkyl, C s cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl- heterocycloalkyl, Ce-n aryl, C7-18 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R10 groups attached to the same atom are combined to form =0; R4, R5, R6 and R7 are each independently hydrogen, Ci-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, halogen, Ci-6 haloalkyl, Ci-6
Figure imgf000048_0002
C3-8 cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-I8 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, wherein at least one of R4, R5, R6 and R7 is not H; alternatively, R4 and R5, R5 and R6, or R6 and R7 are combined with the atoms to which they are each attached to form a C3-6 cycloalkyl,
C3-6 heterocycloalkyl, Ce-n aryl or C5-10 heteroaryl; and R8a, R8b, R8c and R8d are each independently H, Ci-6 alkyl.
[0163] In some embodiments, the present invention provides a method for increasing neuronal plasticity, comprising contacting a neuronal cell with a compound of Formula I, wherein: R1 is hydrogen or Ci-6 alkyl; R3a and R3b are each independently hydrogen, Ci-6 alkyl, Civs cycloalkyl, or C4-14 alkyl-cycloalkyl; R3c is hydrogen or Ci-6 alkyl; alternatively,
R2 and R3b are combined with the atoms to which they are attached to form a C5- 8 heterocycloalkyl substituted with 1 to 3 R9 groups which are each independently hydrogen, Ci-6 alkyl, C vs cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl- heterocycloalkyl, Ce-i2 aryl, C7-I8 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R9 groups attached to the same atom are combined to form =0; alternatively, R2 and R3b are combined with the atoms to which they are attached to form a Cvs cycloalkyl, substituted with 1 to 3 R10 groups which are each independently hydrogen, Ci-6 alkyl, C s cycloalkyl, C3- 14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-I8 alkyl- aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R10 groups attached to the same atom are combined to form =0; R4, R5, R6 and R7 are each independently hydrogen, Ci-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, halogen, Ci-6 haloalkyl, Ci-6 alkylamine, Ci-6 alkoxy, Ci-6 haloalkoxy, -
Figure imgf000049_0001
N (R8b)C(0)N (R8cR8d), -C(0)C(0)N (R8bR8c), -S(0 )R8b, -S(0) N(R8bR8c), C3-8 cycloalkyl, C3- 14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, C6-12 aryl, C7-18 alkyl- aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, wherein at least one of R4, R5, R6 and R7 is not H; R8b, R8c and R8d are each independently H or Ci-6 alkyl; or pharmaceutically acceptable salts and isomers thereof, in an amount sufficient to increase neuronal plasticity of the neuronal cell.
B. Methods of Treating a Brain Disorder
[0164] In some embodiments, the present invention provides a method of treating a disease, including administering to a subject in need thereof, a therapeutically effective amount of a compound of the present invention. In some embodiments, the present invention provides a method of treating a brain disorder, including administering to a subject in need thereof, a therapeutically effective amount of a compound of the present invention. In some embodiments, the present invention provides a method of treating a brain disorder with combination therapy, including administering to a subject in need thereof, a therapeutically effective amount of a compound of the present invention and at least one additional therapeutic agent.
[0165] In some embodiments, 5-HT2A modulators (e.g., 5-HT2A agonists) are used to treat a brain disorder. In some embodiments, the brain disorders comprise decreased neural plasticity, decreased cortical structural plasticity, decreased 5-HT2A receptor content, decreased dendritic arbor complexity, loss of dendritic spines, decreased dendritic branch content, decreased spinogenesis, decreased neuritogenesis, retraction of neurites, or any combination thereof.
[0166] In some embodiments, a compound of the present invention is used to treat brain disorders. In some embodiments, the compounds have, for example, anti- addictive properties, antidepressant properties, anxiolytic properties, or a combination thereof. In some embodiments, the brain disorder is a neuropsychiatric disease. In some embodiments, the neuropsychiatric disease is a mood or anxiety disorder. In some embodiments, brain disorders include, for example, migraine, cluster headache, post-traumatic stress disorder (PTSD), anxiety, depression, schizophrenia, and addiction (e.g., substance abuse disorder). In some embodiments, brain disorders include, for example, migraines, addiction (e.g., substance use disorder), depression, and anxiety.
[0167] In some embodiments, the present invention provides a method of treating a brain disorder, comprising administering to a subject in need thereof a therapeutically effective amount of a compound of Formula I:
Figure imgf000050_0001
or a pharmaceutically acceptable salt thereof, thereby treating the brain disorder, wherein: R1 is hydrogen or Ci-6 alkyl; R3a and R3b are each independently hydrogen, Ci-6 alkyl, CYx cycloalkyl, or C4-14 alkyl-cycloalkyl; R3c is hydrogen or Ci-6 alkyl; alternatively, R2 and R3b are combined with the atoms to which they are attached to form a C5-8 heterocycloalkyl substituted with 1 to 3 R9 groups which are each independently hydrogen, Ci-6 alkyl, C s cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-I8 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R9 groups attached to the same atom are combined to form =0; alternatively, R2 and R3c are combined with the atoms to which they are attached to form a C5-8 cycloalkyl, substituted with 1 to 3 R10 groups which are each independently hydrogen, Ci-6 alkyl, C3-8 cycloalkyl, C3-14 alkyl-cycloalkyl, C4- 10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-I8 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R10 groups attached to the same atom are combined to form =0; R4, R5, R6 and R7 are each independently hydrogen, Ci-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, halogen, Ci-6 haloalkyl, Ci-6 alkylamine, Ci-6 alkoxy, Ci-6 haloalkoxy, -OR8a, -NO2, -CN, - C(0)R8b, -C(0)OR8b, -OC(0)R8b, -OC(0)OR8b, -N(R8bR8c), -N(R8b)C(0)R8c, - C(0)N(R8bR8c), -N (R8b)C(0)0R8c, -0C(0)N(R8bR8c), -N(R8b)C(0)N(R8cR8d), - C(0)C(0)N (R8bR8c), -S(02)R8b, -S(0)2N(R8bR8c), CYx cycloalkyl, C3-14 alkyl-cycloalkyl, C - 10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, C6-i2 aryl, C7-I8 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, wherein at least one of R4, R5, R6 and R7 is not H; alternatively, R4 and R5, R5 and R6, or R6 and R7 are combined with the atoms to which they are each attached to form a C3-6 cycloalkyl, C3-6 heterocycloalkyl, C6-i2 aryl or C5-10 heteroaryl; and R8a, R8b, R8c and R8d are each independently H, Ci-6 alkyl.
[0168] In some embodiments, the present invention provides a method of treating a brain disorder, comprising administering to a subject in need thereof a therapeutically effective amount of a compound of Formula I, wherein: R1 is hydrogen or Ci-6 alkyl; R3a and R3b are each independently hydrogen, Ci-6 alkyl, CYx cycloalkyl, or C4-14 alkyl-cycloalkyl; R3c is hydrogen or Ci-6 alkyl; alternatively, R2 and R3b are combined with the atoms to which they are attached to form a CYx heterocycloalkyl substituted with 1 to 3 R9 groups which are each independently hydrogen, Ci-6 alkyl, CYx cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10
heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, C6-i2 aryl, C7-I8 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R9 groups attached to the same atom are combined to form =0; alternatively, R2 and R3b are combined with the atoms to which they are attached to form a C5-8 cycloalkyl, substituted with 1 to 3 R10 groups which are each independently hydrogen, Ci-6 alkyl, C3-8 cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl- heterocycloalkyl, C6-i2 aryl, CYi x alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R10 groups attached to the same atom are combined to form =0; R4, R5, R6 and R7 are each independently hydrogen, Ci-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, halogen, Ci-6 haloalkyl, Ci-6
Figure imgf000051_0001
C3-8 cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, C6-i2 aryl, C7-18 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, wherein at least one of R4, R5, R6 and R7 is not H; R8b, R8c and R8d are each independently H or Ci-6 alkyl; or pharmaceutically acceptable salts and isomers thereof, thereby treating the brain disorder
[0169] In some embodiments, the brain disorder is a neurodegenerative disorder,
Alzheimer’s, Parkinson’s disease, psychological disorder, depression, addiction, anxiety, post-traumatic stress disorder, treatment resistant depression, suicidal ideation, major depressive disorder, bipolar disorder, schizophrenia, stroke, traumatic brain injury, or substance use disorder.
[0170] In some embodiments, the brain disorder is a neurodegenerative disorder,
Alzheimer’s, or Parkinson’s disease. In some embodiments, the brain disorder is a psychological disorder, depression, addiction, anxiety, or a post-traumatic stress disorder. In some embodiments, the brain disorder is depression. In some embodiments, the brain disorder is addiction. In some embodiments, the brain disorder is treatment resistant depression, suicidal ideation, major depressive disorder, bipolar disorder, schizophrenia, stroke, traumatic brain injury or substance use disorder. In some embodiments, the brain disorder is treatment resistant depression, suicidal ideation, major depressive disorder, bipolar disorder, schizophrenia, or substance use disorder. In some embodiments, the brain disorder is treatment resistant depression, suicidal ideation, major depressive disorder, bipolar disorder, or substance use disorder. In some embodiments, the brain disorder is stroke or traumatic brain injury. In some embodiments, the brain disorder is schizophrenia. In some embodiments, the brain disorder is alcohol use disorder.
[0171] In some embodiments, the method further comprises administering one or more additional therapeutic agent that is lithium, Olanzapine (Zyprexa), Quetiapine (Seroquel), Risperidone (Risperdal), Ariprazole (Abilify), Ziprasidone (Geodon), Clozapine (Clozaril), divalproex sodium (Depakote), lamotrigine (Lamictal), valproic acid (Depakene), carbamazepine (Equetro), topiramate (Topamax), levomilnacipran (Fetzima), duloxetine (Cymbalta, Yentreve), venlafaxine (Effexor), citalopram (Celexa), fluvoxamine (Luvox), escitalopram (Lexapro), fluoxetine (Prozac), paroxetine (Paxil), sertraline (Zoloft), clomipramine (Anafranil), amitriptyline (Elavil), desipramine (Norpramin), imipramine (Tofranil), nortriptyline (Pamelor), phenelzine (Nardil), tranylcypromine (Parnate), diazepam (Valium), alprazolam (Xanax), or clonazepam (Klonopin).
[0172] In some embodiments, the compounds of the present invention are used in combination with the standard of care therapy for a neurological disease described herein. Non- limiting examples of the standard of care therapies, may include, for example, lithium, olanzapine, quetiapine, risperidone, ariprazole, ziprasidone, clozapine, divalproex sodium, lamotrigine, valproic acid, carbamazepine, topiramate, levomilnacipran, duloxetine, venlafaxine, citalopram, fluvoxamine, escitalopram, fluoxetine, paroxetine, sertraline, clomipramine, amitriptyline, desipramine, imipramine, nortriptyline, phenelzine, tranylcypromine, diazepam, alprazolam, clonazepam, or any combination thereof. Non limiting examples of standard of care therapy for depression are sertraline, fluoxetine, escitalopram, venlafaxine, or aripiprazole. Non-limiting examples of standard of care therapy for depression are citralopram, escitalopram, fluoxetine, paroxetine, diazepam, or sertraline.
C. Methods of increasing at least one of translation, transcription, or secretion of neurotrophic factors
[0173] Neurotrophic factors refers to a family of soluble peptides or proteins which support the survival, growth, and differentiation of developing and mature neurons. Increasing at least one of translation, transcription, or secretion of neurotrophic factors can be useful for, but not limited to, increasing neuronal plasticity, promoting neuronal growth, promoting
neuritogenesis, promoting synaptogenesis, promoting dendritogenesis, increasing dendritic arbor complexity, increasing dendritic spine density, and increasing excitatory synapsis in the brain. In some embodiments, increasing at least one of translation, transcription, or secretion of neurotrophic factors can increasing neuronal plasticity. In some embodiments, increasing at least one of translation, transcription, or secretion of neurotrophic factors can promoting neuronal growth, promoting neuritogenesis, promoting synaptogenesis, promoting dendritogenesis, increasing dendritic arbor complexity, and/or increasing dendritic spine density.
[0174] In some embodiments, 5-HT2A modulators (e.g., 5-HT2A agonists) are used to increase at least one of translation, transcription, or secretion of neurotrophic factors. In some embodiments, a compound of Formula I or Formula (la) described herein is used increase at least one of translation, transcription, or secretion of neurotrophic factors. In some embodiments, increasing at least one of translation, transcription or secretion of neurotrophic factors treats a migraine, headaches (e.g., cluster headache), post-traumatic stress disorder (PTSD), anxiety, depression, neurodegenerative disorder, Alzheimer’s disease, Parkinson’s disease, psychological disorder, treatment resistant depression, suicidal ideation, major depressive disorder, bipolar disorder, schizophrenia, stroke, traumatic brain injury, and addiction (e.g., substance use disorder).
[0175] In some embodiments, the experiment or assay used to determine increase translation of neurotrophic factors includes ELISA, western blot, immunofluorescence assays, proteomic experiments, and mass spectrometry. In some embodiments, the experiment or assay used to determine increase transcription of neurotrophic factors includes gene expression assays, PCR, and microarrays. In some embodiments, the experiment or assay used to determine increase secretion of neurotrophic factors includes ELISA, western blot, immunofluorescence assays, proteomic experiments, and mass spectrometry.
[0176] In some embodiments, the present invention provides a method for increasing at least one of translation, transcription or secretion of neurotrophic factors, comprising contacting a neuronal cell with a compound of Formula I:
Figure imgf000054_0001
or a pharmaceutically acceptable salt thereof, in an amount sufficient to increase neuronal plasticity of the neuronal cell, wherein: R1 is hydrogen or Ci-6 alkyl; R3a and R3b are each independently hydrogen, Ci-6 alkyl, Cvx cycloalkyl, or C4-14 alkyl-cycloalkyl; R3c is hydrogen or Ci-6 alkyl; alternatively, R2 and R3b are combined with the atoms to which they are attached to form a C5-8 heterocycloalkyl substituted with 1 to 3 R9 groups which are each independently hydrogen, Ci-6 alkyl, Cvx cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10
heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-18 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R9 groups attached to the same atom are combined to form =0; alternatively, R2 and R3c are combined with the atoms to which they are attached to form a C5-8 cycloalkyl, substituted with 1 to 3 R10 groups which are each independently hydrogen, Ci-6 alkyl, C s cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl- heterocycloalkyl, Ce-n aryl, C7-18 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R10 groups attached to the same atom are combined to form =0; R4, R5, R6 and R7 are each independently hydrogen, Ci-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, halogen, Ci-6 haloalkyl, Ci-6
Figure imgf000054_0002
C3-8 cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-18 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, wherein at least one of R4, R5, R6 and R7 is not H; alternatively, R4 and R5, R5 and R6, or R6 and R7 are combined with the atoms to which they are each attached to form a C3-6 cycloalkyl, C3-6 heterocycloalkyl, Ce-n aryl or C5-10 heteroaryl; and R8a, R8b, R8c and R8d are each independently H, Ci-6 alkyl.
[0177] In some embodiments, the present invention provides a method for increasing at least one of translation, transcription or secretion of neurotrophic factors, comprising contacting a neuronal cell with a compound of Formula I, wherein: R1 is hydrogen or Ci-6 alkyl; R3a and R3b are each independently hydrogen, Ci-6 alkyl, C vx cycloalkyl, or C4-14 alkyl- cycloalkyl; R3c is hydrogen or Ci-6 alkyl; alternatively, R2 and R3b are combined with the atoms to which they are attached to form a C5-8 heterocycloalkyl substituted with 1 to 3 R9 groups which are each independently hydrogen, Ci-6 alkyl, CYx cycloalkyl, C3-14 alkyl- cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-18 alkyl-aryl, C5- 10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R9 groups attached to the same atom are combined to form =0; alternatively, R2 and R3b are combined with the atoms to which they are attached to form a C5-8 cycloalkyl, substituted with 1 to 3 R10 groups which are each independently hydrogen, Ci-6 alkyl, C vx cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10
heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-18 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R10 groups attached to the same atom are combined to form =0; R4, R5, R6 and R7 are each independently hydrogen, Ci-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, halogen, Ci-6 haloalkyl, Ci-6 alkylamine, Ci-6 alkoxy, Ci-6 haloalkoxy, -OR8a, -NO2, -CN, -
Figure imgf000055_0001
C(0)C(0)N (R8bR8c), -S(02)R8b, -S(0)2N(R8bR8c), C3-8 cycloalkyl, C3-14 alkyl-cycloalkyl, C - 10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-18 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, wherein at least one of R4, R5, R6 and R7 is not H; R8b, R8c and R8d are each independently H or Ci-6 alkyl; or pharmaceutically acceptable salts and isomers thereof, in an amount sufficient to increase neuronal plasticity of the neuronal cell.
VII. Examples
[0178] Chemistry (General). All reagents were obtained commercially unless otherwise noted. Reactions were performed using glassware that was oven dried (120°C) unless otherwise stated. Air- and moisture-sensitive liquids and solutions were transferred via syringe or stainless-steel cannula. Organic solutions were concentrated under reduced pressure (~5 Torr) by rotary evaporation. Solvents were purified by passage under 12 psi N2 through activated alumina columns. Chromatography was performed using Fisher Chemical™ Silica Gel Sorbent (230-400 Mesh, Grade 60). Compounds purified by chromatography were typically applied to the adsorbent bed using the indicated solvent conditions with a minimum amount of added dichloromethane as needed for solubility. Thin layer chromatography (TLC) was performed on Merck silica gel 60 F254 plates (250 pm). Visualization of the developed chromatogram was accomplished by fluorescence quenching or by staining with butanolic ninhydrin, aqueous potassium permanganate, ethanolic vanillin, or aqueous ceric ammonium molybdate (CAM).
[0179] Nuclear magnetic resonance (NMR) spectra were acquired on either a Bruker 400 operating at 400 and 100 MHz or a Varian-600 operating at 600 and 150 MHz for 11 1 and 13C, respectively, and are referenced internally according to residual solvent signals. Data for 1H NMR are recorded as follows: chemical shift (d, ppm), multiplicity (s, singlet; br s, broad singlet; d, doublet; t, triplet; q, quartet; quint, quintet; sext, sextet; m, multiplet), integration, and coupling constant (Hz). Data for 13C NMR are reported in terms of chemical shift (d, ppm). Infrared spectra were recorded using a Thermo Scientific Nicolet iS lO spectrometer with Smart iTX Accessory (diamond ATR) and are reported in frequency of absorption. Low-resolution mass spectra were obtained using a Waters Acuity Arc LC-MS.
[0180] The specific procedures used to synthesize the compounds reported in this manuscript are detailed below along with characterization data. Spectral data ( ' l l and 13C NMR spectra) for each compound tested in biological assays is included.
Example 1. Synthesis and Biological Activity of Ibogalogs
[0181] The pharmacophore of ibogaine is depicted in FIG. 1A. A series of
tetrahydroazepine-containing compounds that lacked the isoquinuclidine characteristic of ibogaine (FIG. IB) was synthesized.
[0182] Embryonic rat cortical neurons were treated for a short period of time (1 h) followed by a suitable growth period (71 h) to measure the psychoplastogenic effects of the compounds described herein. Using this short treatment protocol (Dunlap, et al.
Identification of Psychoplastogenic N,N-Dimethylaminoisotryptamine (isoDMT) Analogs Through Structure -Activity Relationship Studies. J. Med. Chem., 2019), ibogaine
demonstrates psychoplastogenic effects as measured via Sholl analysis. Therefore, the effects of ibogalogs 8-17 on dendritic growth was assessed. [0183] Except for 11a, ibogalogs containing the isoquinuclidine but lacking the tetrahydroazepine ring (8-10 and lib) were weak psychoplastogens or did not promote neuronal growth compared to the vehicle (VEH) control (FIG. 5 and FIG. 6). Ibogalogs lacking the isoquinuclidine but retaining the tetrahydroazepine (13-16) were efficacious (FIG. 5 and FIG. 6). Indole substitution at C5 with fluorine or chlorine was tolerated, but a more sterically demanding bromine substituent was not. IBG (13) performed comparably to ibogaine despite having a drastically simplified chemical structure.
[0184] A HTR assay using 5-MeO-DMT (10 mg/kg) as a positive control (FIG. 2B) was used to evaluate the hallucinogenic potential of IBG and tabemanthalog (TBG). While 5- MeO-DMT produces a robust HTR, its conformationally restricted analog IBG exhibits significantly reduced hallucinogenic potential. The 6-methoxy substituent of TBG did not display hallucinogenic potential as measured by the HTR assay. For these in vivo studies, the fumarate salts of IBG and TBG were utilized. Unlike ibogaine hydrochloride, they are readily soluble in 0.9% saline up to 40 mg/mL (FIG. 2C). Ibogaine’s lack of water solubility (FIG. 7) is a potential issue not only for clinical formulation, but also for administration to animals during preclinical testing.
[0185] The lipophilicity of ibogaine not only poses practical issues for its administration, it is likely a major factor contributing to its toxicity and adverse cardiac effects. Ibogaine inhibits hERG channels with an IC50 of approximately 1 mM (FIG. 2D). In contrast, IBG and TBG are approximately 10- and 100-fold less potent than ibogaine, respectively (IBG IC50 = 19.3 mM; TBG IC50 = 148 pM), indicating a lower potential for cardiotoxicity.
Administration of ibogaine to immobilized larval zebrafish resulted in a visually noticeable decrease in heart rate (FIG. 2E) and an increased likelihood for inducing arrhythmias as measured by the ratio of atrium to ventricle beats per minute (BPM) (FIG. 2F). Neither IBG nor TBG induced these undesirable phenotypes.
[0186] To compare the acute behavioral effects of ibogaine, IBG, and TBG, larval zebrafish for 1 h were treated and then quantified their movement activity during a battery of light and acoustic stimuli as described previously (FIG. 8). With increasing concentration, ibogaine, noribogaine, and the hERG inhibitors haloperidol, sertindole, and terfenadine become more phenotypically distinct from the vehicle control, while more closely resembling the lethal control (eugenol, 100 pM) (FIG. 9). At the highest concentrations tested (200 pM), noribogaine and a toxic pesticide (endosulfan) were phenotypically consistent with the lethal control. In contrast, IBG and TBG did not produce this phenotype. A machine learning approach showed that, at the highest concentrations, IBG and TBG caused movement responses that were more similar to vehicle controls than to ibogaine, noribogaine, or lethal controls (FIG. 2G). This suggests that the novel compounds have superior acute safety profiles.
[0187] As ibogaine is known to cause seizures at very high doses, seizurogenic potential using larval zebrafish expressing GCaMP5 was assessed. Neither ibogaine nor TBG produced excessive neural activity as was observed following treatment with the known seizure-inducing compound pentylenetetrazole (PTZ) (FIG. 10).
[0188] Finally, in a well-established zebrafish developmental toxicity assay, Ibogaine (100 mM) significantly increased malformations and mortality at 2 and 5 days post- fertilization (dpf), respectively (FIG. 2H and FIG. 21). At both timepoints, the proportion of viable to non- viable fish was significantly different from vehicle control (Fisher’s exact test, p < 0.0001). Ibogaine-treated animals suffered from numerous malformations. Noribogaine treatment resulted in greater survival, but the majority of the animals exhibited yolk sac and/or pericardial edemas. In sharp contrast, both IBG and TBG treatments (100 mM) resulted in significantly fewer non-viable fish than ibogaine or noribogaine treatments at both 2 and 5 dpf (Fisher’s exact test, p < 0.0001 for ibogaine vs. IBG and ibogaine vs. TBG at both 2 and 5 dpf; p < 0.0001 for noribogaine vs. IBG at both 2 and 5 dpf and noribogaine vs. TBG at 2 dpf; p = 0.0083 for NOR vs. TBG at 5 dpf). Moreover, the effects of compound- induced malformations and/or death were both time- (FIG. 2J) and concentration-dependent (FIG. 11). Importantly, reducing the concentration of TBG from 100 to 66 pM resulted in a proportion of viable to non-viable fish that was statistically indistinguishable from vehicle control after 5 dpf (Fisher’s exact test, p = 0.3864).
[0189] To validate the targets of IBG and TBG, a panel of serotonin (5-HT) and opioid receptor functional assays assessing canonical GPCR signaling was performed. Unlike noribogaine, IBG and TBG showed weak or no opioid agonist activity (FIG. 12 and FIG.
13). However, IBG and TBG demonstrated potent agonist activity at human (FIG. 2K) and mouse 5-HT2A receptors (FIG. 12). Many 5-HT2A agonists, such as 5-MeO-DMT, are also agonists of 5-HT2B receptors, which can lead to cardiac valvulopathy. Fortunately, IBG and TBG act as antagonists at 5-HT2B receptors (FIG. 2K). When profiled across the 5-HT receptorome, both compounds displayed distinct profiles as compared to 5-MeO-DMT (FIG. 12, FIG. 13, and FIG. 14), including weaker 5-HT1A and 5-HT2C agonist activity. In fact, IBG and TBG exhibit more selective, and potentially safer profiles than the less
conformationally restricted 5-MeO-DMT with a strong preference for activating 5-HT2A receptors over similar 5-HT receptors or opioid receptors. Example 2. Compounds 4a and 4b
[0190] To a solution of pyridine (10.0 g, 126.4 mmol, 1.0 equiv) and sodium borohydride (4.8 g, 126.4 mmol, 1.0 equiv) in MeOH (56 mL, 2.3 M) at -78oC was added
benzylchloroformate (18.0 mL, 21.6 g, 126.4 mmol, 1.0 equiv) via slow dropwise addition. Gas evolution was observed. The mixture was stirred for 3h at -78°C, diluted with Et20 (100 mL) and H2O (50 mL), and warmed to room temperature. The aqueous layer was extracted with Et20 (3 x 50 mL). The combined organic extracts were washed with brine (3 x 50 mL), dried over Na2SO t, and concentrated under reduced pressure to yield a clear oil. The produce was passed through a short plug of silica gel using hexanes:EtOAc (95:5) as the eluent. The resulting filtrate was concentrated under reduced pressure. After purging with nitrogen, methyl vinyl ketone (10.5 mL, 126.4 mmol, 1.0 equiv) was added to the flask and the neat solution was heated to 80°C for 24h. After cooling the mixture to room temperature, MeOH (250 mL) was added followed by 25% w/w aqueous NaOMe (2.4 mL, 8.9 mmol, 0.07 equiv). The resulting mixture was stirred for 15 min, quenched with H2O (5 mL), then concentrated under reduced pressure. The residue was dissolved in DCM (250 mL) and then washed with H2O (100 mL) followed by brine (50 mL). The organic layer was dried over Na2S04 and concentrated under reduced pressure. The produce was purified by chromatography on silica gel (3: 1 hexanes :EtO Ac) to give a clear oil containing a mixture of 4a and 4b (26.5 g, 73% over 3 steps, ~1 : 1 exo:endo as a mixture of rotamers).
Figure imgf000059_0001
Me A)
4a 4b
[0191] ¾ NMR (CDCI3, 400 MHz) d 7.4-7.2 (m, 5H), 6.57-6.23 (m, 2H), 5.22-4.94 (m,
3H), 3.33 (t, ¾ J = 10.3 Hz), 3.18-2.58 (m, 3H), 2.35-2.05 (m, 3H), 1.91-1.66 (m, 1H), 1.64-1.23 (m, lH) ppm; IR (Smart iTX Diamond) v 3060, 2957, 2878, 1699, 1417, 1366, 1337, 1300, 1279, 1113, 764, 699 cm 1; LC-MS (ES+) calcd for C17H20NO3 [M + H] 286.14 found 286.22. Example 3. Compounds 5a and 5b
[0192] To a mixture of 4a and 4b (4.8 g, 17.0 mmol, 1.0 equiv) in anhydrous THF (13 mL, 1.3 M) was added p-toluenesulfonyl hydrazide (3.16 g, 17.0 mmol, 1 equiv). The mixture was refluxed for 15-20 hour until starting material was consumed as determined by TLC. The reaction mixture was concentrated under reduced pressure, siluted with Et20 (20 mL), and sonicated for 15 min. The white precipitate was filtered and washed with Et20 (4 x 5 mL) to yield 5b. The filtrate was concentrated under reduced pressure and purified by
chromatography on silica gel (gradient elution: 5: 1 1 : 1 hexanes :EtO Ac) to give 5a (75%; exo = 5.88 g, endo = 7.33 g, mixture of exo and endo = 2.62 g).
Figure imgf000060_0001
[0193] Isolated as a white solid (5.88 g, mixture of rotamers); 11 1 NMR (CDCI3, 400 MHz) d 7.80 (dd, 2H, J = 20.1, 8.3 Hz), 7.43-7.26 (m, 4H), 7.25-7.1 1 (m, 3H), 6.49-6.36 (m, 2H), 5.10 (dd, 1H, J = 36.0, 12.3 Hz), 4.80-4.57 (m, 2H), 3.02 (dd, 1H, J = 10.0, 2.1 Hz), 2.92- 2.84 (m, 1H), 2.75-2.67(m, 1H), 2.48-2.13 (m, 5H), 1.89 (s, 2H), 1.59 (s, 1H), 1.47 (s, 1H), 1.39-1.27 (m, 1H) ppm; IR (Smart iTX Diamond) v 3207, 2952, 2876, 1675, 1418, 1368,
1337, 1303, 1258, 1 165, 1 1 17, 1030, 917, 813, 763, 705, 667, 552 cm 1; LC-MS (ES+) calcd for C24H28N2O4S [M + H] 454.18 found 454.33.
Figure imgf000060_0002
[0194] Isolated as a white solid (7.33 g, mixture of rotamers); 11 1 NMR (CDCb, 400 MHz) d 7.79 (t, 2H, J = 8.9 Hz), 7.42-7.27 (m, 6H), 7.09 (d, 1H, J = 8.0 Hz), 6.21 (t, 1H, J = 7.3 Hz), 6.08-6.00 (m, 1H), 5.23 -5.05 (m, 2H), 4.93 -4.82 (m, 1H), 3.33-3.23 (m, 1H), 3.06- 2.96 (m, 1H), 2.96-2.86 (m, 1H), 2.81 -2.71 (m, 1H), 2.41 (s, 3H), 1.93 - 1.46 (m, 6H) ppm;
IR (Smart iTX Diamond) v 3105, 2939, 2878, 1671, 1446, 1420, 1372, 1338, 1322, 1298, 1280, 1262, 1241, 1 167, 976, 932, 818, 804, 766, 724, 670, 550 cm4; LC-MS (ES+) calcd for C24H28N2O4S [M + H] 454.18 found 454.33. Example 4. Compounds 6a and 6b
[0195] To a solution of 5a or 5b (3.78 g, 8.35 mmol, 1.0 equiv) in THF (16.7 mL, 2 M) was added sodium cyanoborohydride (2.1 g, 33.4 mmol, 4 equiv) and p-toluenesulfonic acid (159 mg, 0.835 mmol, 0.1 equiv). The mixture was refluxed for 18 h, diluted with H2O (30 mL), and extracted with cyclohexane (5 x 25 mL). The combined organic extracts were washed with H2O (20mL), saturated aqueous NaHCCb (20 mL), and brine (20 mL) before being dried over Na2S04 and concentrated under reduced pressure to yield either 6a or 6b.
Figure imgf000061_0001
[0196] Isolated as a clear oil (2.01g, 57%, mixture of rotamers); !H NMR (CDCb, 400 MHz) d 7.41 -7.27 (m, 5H), 6.55-6.38 (m, 1H), 6.33 (q, 1H, J = 7.7 Hz), 5.22-5.02 (m, 2H),
4.58 (d, 1H, J = 6.1 Hz), 3.30-3.20 (m, 1H), 3.08-2.96 (m, 1H), 2.75-2.58 (m, 1H), 1.69-
1.59 (m, 1H), 1.53 - 1.30 (m, 3H), 1.05-0.98 (m, 1H), 0.98-0.84 (m, 3H) ppm; IR (Smart iTX Diamond) v 3052, 2957, 2873, 1699, 1417, 1335, 1295, 1 106, 986, 764, 699 cm 1; LC-MS (ES+) calcd for C17H21NO2 [M + H] 272.17 found 272.29.
Figure imgf000061_0002
[0197] Isolated as a clear oil (2.66 g, 62%, mixture of rotamers); 11 1 NMR (CDCI3, 400 MHz) d 7.39-7.26 (m, 5H), 6.42-6.22 (m, 2H), 5.18-5.08 (m, 2H), 4.73 -4.55 (m, 1H), 3.32- 3.20 (m, 1H), 3.04-2.95 (m, 1H), 2.77-2.62 (m, 1H), 2.05- 1.91 (m, 1H), 1.87- 1.77 (m, 1H), 1.28 - 1.11 (m, 1H), 1.05-0.82 (m, 5H) ppm; IR (Smart iTX Diamond) v 3052, 2958, 2931, 2874, 1699, 1416, 1333, 1301, 1276, 1 1 1 1, 977, 766, 698 cm 1. LC-MS (ES+ ) calcd for
C17H21NO2 [M + H] 272.17 found 272.29.
Example 5. Compounds 8a and 8b
[0198] A solution of either 6a or 6b (132 mg, 0.95 mmol, 1.0 equiv) in acetic acid (0.5 mL, 1.9 M) was heated to 55°C prior to the addition of sodium borohydride (165 mg, 4.37 mmol, 4.6 equiv). Gas evolution was observed. The mixture was stirred at 55°C for 10 h, diluted with H2O (2 mL), cooled to 0°C and basified with solid NaOH (until pH = 14). The aqueous layer was extracted with Et20 (4 x 10 mL), dried over Na2SO t, and concentrated under reduced pressure. The residue was purified by chromatography on silica gel (100: 1
DCM:MeOH with 0.5% NH4OH) to afford product.
Figure imgf000062_0001
[0199] Isolated as a yellow oil (12 mg, 7%); ¾ NMR (CDC , 400 MHz) d 3.05-2.96 (m,
1H), 2.47 (q, 2H, J = 7.2 Hz), 2.37 (t, 1H, J = 2.4 Hz), 2.20 (dt, 1H, J = 9.3, 1.5 Hz), 2.00- 1.89 (m, 1H), 1.73 - 1.64 (m, 1H), 1.64- 1.58 (m, 1H), 1.56- 1.36 (m, 5H), 1.32- 1.23 (m, 1H), 1.19- 1.11 (m, 1H), 1.00 (t, 3H, J = 7.2 Hz), 0.86 (t, 3H, J = 7.1 Hz) ppm; 13C NMR (CDCb, 100 MHz) d 56.67, 51.92, 49.18, 40.36, 32.76, 27.15, 26.89, 25.34, 21.52, 13.64, 1 1.98 ppm; IR (Smart iTX Diamond) v 2955, 2926, 2857, 2793, 1458, 1372, 1259, 1 100, 1048, 799 cm 1; LC-MS (ES+ ) calcd for C11H21N [M + H] 168.18 found 168.33.
Figure imgf000062_0002
[0200] Isolated as a yellow oil (71.5 mg, 47%); !H NMR (CDCb, 400 MHz) d 2.76 (dt, 1H, J = 9.8, 2.7 Hz), 2.65-2.46 (m, 3H), 2.45-2.39 (m, 1H), 1.91 - 1.70 (m, 3H), 1.66- 1.55 (m, 3H), 1.47- 1.37 (m, 1H), 1.35- 1.20 (m, 2H), 1.07 (d, 3H, J = 14.4 Hz), 0.99 (dt, 1H, J = 6.3,
2.2 Hz), 0.88 (t, 3H, J = 7.4 Hz) ppm; 13C NMR (CDCb, 100 MHz) d 55.71, 52.90, 49.55, 35.32, 32.94, 27.67, 26.63, 25.30, 20.02, 13.47, 12.19 ppm; IR (Smart iTX Diamond) v 2958, 2931, 2872, 2860, 2792, 1464, 1371, 1217, 1 161, 1094, 819, 756 cm4; LC-MS (ES+) calcd for C11H21N [M + H] 168.17 found 168.25. Example 6. Preparation of 5-Substitiited Tryptophols
[0201] General Procedure for Preparing Hydrazines. Most hydrazines were
commercially available, however the 4-iodo and 4-benzyloxy hydrazines were synthesized in house according to the following procedure. A 0.5 M solution of 4-substituted aniline (3.99 mmol, 1 equiv) in aqueous concentrated HC1 was cooled to 0°C before the addition of 2.0 M aqueous NaNC (3.91 mmol, 0.98 equiv). The solution was stirred for 20 min at 0°C. Next, a solution of SnCk 2¾0 (10.4 mmol, 2.6 equiv) dissolved in concentrated aqueous HC1 (2.4 mL) was added. The mixture was stirred for 2 h, warmed to room temperature, filtered, and rinsed with H2O and Et20. The solids were dried under reduced pressure and used immediately without further purification.
[0202] All 5-substituted tryptophols were prepared from the corresponding 4-substituted hydrazines as outlined here. General Scheme:
Figure imgf000063_0001
[0203] A solution of phenyl hydrazine (0.5 g, 3.46 mmol, 1 equiv) in 10 mL of a 1 : 1 mixture of DMA and 4% aqueous H2SO4 was heated to 100°C. To this solution was added 1 ,2-dihydrofuran (0.29 mL, 266 mg, 3.8 mmol, 1.1 equiv) via dropwise addition. The resulting mixture was stirred at 100°C until starting material was consumed as determined by TLC (~3 h). The reaction was then cooled to room temperature and diluted with EtOAc (lOmL). The aqueous layer was extracted with EtOAc (5 x 10 mL). The combined organic extracts were washed with 5% aqueous LiCl (15 mL), saturated aqueous NaHC03(15 mL), and H2O (15 mL) before being dried over Na2S04. The resulting product was purified by chromatography on silica gel (3: 1 hexanes: EtOAc) to yield the desired tryptophol as a brown solid (258 mg, 92%); *H NMR (CDCI3, 400 MHz) d 8.23 (s, 1H), 7.70-7.59 (m, 1H), 7.36 (d, 1H, J = 8.1 Hz), 7.25 -7.19 (m, 1H), 7.15 (td, 1H, J = 7.6, 7.1, 1.0 Hz), 7.09-7.00 (m, 1H), 3.91 (t, 2H, J = 6.4 Hz), 3.04 (t, 2H, J = 6.4 Hz), 1.86 (s, 1H) ppm; 13C NMR (CDCI3, 100 MHz) d 136.47, 127.44, 122.54, 122.23, 1 19.49, 1 18.86, 1 12.27, 1 1 1.26, 62.64, 28.77 ppm; IR (Smart iTX Diamond) v 3390, 3322, 3059, 2933, 2905, 2863, 1456, 1424, 1352, 1338, 1229, 1094, 1045, 1005, 738, 591 cm 1; LC-MS (ES+) calcd for CioHnNO [M + H] 162.09 found 162.21.
Figure imgf000063_0002
[0204] A solution of /r-MeO-pheny I hydrazine (2g, 1 1.45 mmol, 1 equiv) in 32.6 mL of a 1 : 1 mixture of DMA and 4% aqueous H2SO4 was heated to 100°C. To this solution was added 1 ,2-dihydrofuran (0.95 mL, 0.883 g, 12.6 mmol, 1.1 equiv) via dropwise addition. The resulting mixture was stirred at 100°C until starting material was consumed as determined by TLC (~5 h). The reaction was then cooled to room temperature and diluted with EtOAc (20 mL). The aqueous layer was extracted with EtOAc (4 x 25 mL). The combined organic extracts were washed with 5% aqueous LiCl (20 mL), saturated aqueous NaHC03 (20 mL), and H2O (20 mL) before being dried over Na2SO t. The resulting product was purified by chromatography on silica gel (gradient elution: 3 : 1 - 1 : 1 hexanes: EtOAc) to yield the desired product as a yellow oil (1.71 g, 78%); !H NMR (CDCL, 400 MHz) d 8.01 (s, 1H), 7.25 (s, 1H), 7.06 (d, 2H, J = 2.5 Hz), 6.88 (dd, 1H, J = 8.8, 2.4 Hz), 3.90 (t, 2H, J = 6.4 Hz), 3.87 (s, 3H), 3.01 (t, 2H, J = 6.4 Hz), 1.70 (s, 1H) ppm; 13C NMR (CDCI3, 100 MHz) d 154.09, 131.70, 127.91, 123.46, 112.45, 1 12.09, 1 1 1.97, 100.76, 62.67, 56.06, 28.82 ppm; IR (Smart iTX Diamond) v 3409, 2938, 2831, 1624, 1584, 1486, 1456,1440, 1214, 1067, 1043, 922, 798 cm 1; LC-MS (ES+) calcd for C11H13NO2 [M + H] 192.10 found 192.27.
Figure imgf000064_0001
[0205] A solution of /?-l3nO-phenyl hydrazine (398 mg, 1.59 mmol, 1 equiv) in 4.54 mL of a 1 : 1 mixture of DMA and 4% aqueous H2SO4 was heated to 100°C. To this solution was added 1 ,2-dihydrofuran (0.13 mL, 123 mg, 1.75 mmol, 1.1 equiv) via dropwise addition. The resulting mixture was stirred at 100°C until starting material was consumed as determined by TLC (~3 h). The reaction was then cooled to room temperature and diluted with EtOAc (lOmL). The aqueous layer was extracted with EtOAc (4 x 10 mL). The combined organic extracts were washed with 5% aqueous LiCl (10 mL), saturated aqueous NaHC03 (10 mL), and H2O (2 x 10 mL) before being dried over Na2SO t. The resulting product was purified by chromatography on silica gel (gradient elution: 3 : 1 - 1 : 1 hexanes: EtOAc) to yield the desired product as a brown oil (160 mg, 38%); ¾ NMR (CDCb, 400 MHz) d 7.95 (s, 1H), 7.49 (d, 2H, J = 7.4 Hz), 7.43 -7.30 (m, 3H), 7.26 (s, 1H), 7.15 (d, 1H, J = 2.3 Hz), 7.06 (d, 1H, J =
2.2 Hz), 6.96 (dd, 1H, J = 8.8, 2.4 Hz), 5.12 (s, 2H), 3.89 (t, 2H, J = 6.3 Hz), 3.00 (t, 2H, J =
6.3 Hz), 1.59 (s, 1H) ppm; 13C NMR (CDCI3, 100 MHz) d 153.25, 137.61, 131.79, 128.53, 127.85, 127.82, 127.63, 123.34, 1 13.13, 1 12.07, 1 1 1.93, 102.44, 71.04, 62.59, 28.77 ppm; IR (Smart iTX Diamond) v 3419, 3062, 3032, 2929, 2878, 1624, 1582, 1483, 1454, 1381, 1293, 1219, 1 194, 1064, 1043, 1025, 797, 740, 698 cm 1; LC-MS (ES+) calcd for CI7HI8NC>2 [M + H] 268.13 found 268.24. Example 7. Compounds 9a and 9b
[0206] First, the hydroxyl groups of the tryptophols shown above were converted to the iodides. To accomplish this transformation, a solution of iodine (251 mg, 0.99 mmol, 1.4 equiv) and triphenyl phosphine (260 mg, 0.99 mmol, 1.4 equiv) in DCM (2.75 mL, 0.25 M) was cooled to 0°C. Next, a tryptophol (0.71 mmol, 1.0 equiv) was added dropwise. The solution was stirred until starting material had been consumed as determined by TLC (~5 h). The reaction mixture was concentrated under reduced pressure to afford the alkyl iodide, which was used immediately without further purification.
[0207] In a separate flask, a mixture of 6a or 6b (271 mg, 1.0 mmol, 1.0 equiv) and 10% Pd/C (85 mg, 0.08 mmol, 0.08 equiv) was stirred in MeOH (5 mL, 0.2 M) under an atmosphere of N2. The flask was then purged with ¾ gas and stirred vigorously under an atmosphere of ¾ until the starting material had been consumed as determined by TLC (~3 h). The mixture was filtered over a pad of celite, which was washed with 50 mL of MeOH containing 1% aqueous NH4OH. The filtrate was then dried over Na2S04 and concentrated under reduced pressure to yield a clear oil. The oil was immediately dissolved in DMF (2.5 mL, 0.4 M) and added to a flask containing the unpurified alkyl iodide (271 mg, 1.0 mmol,
1.0 equiv, generated as described above) and solid NaHC03 (168 mg, 2 mmol, 2 equiv.). The mixture was heated at 80°C for 20h before being cooled to room temperature and diluted with 1M aqueous Na2CC>3 (15 mL) and EtOAc (15 mL). The aqueous layer was extracted with EtOAc (3 x 10 mL). The combined organic extracts were washed with H2O (10 mL), 5% aqueous LiCl (10 mL), and brine (10 mL) before being dried over Na2S04 and concentrated under reduced pressure. The product was purified by chromatography on silica gel (100: 1 DCM:MeOH with 0.5% NH4OH).
Figure imgf000065_0001
[0208] Isolated as a tan solid (30 mg, 24%, 2 steps); ¾ NMR (CDCb, 400 MHz) d 7.95 (s,
1H), 7.64 (d, 1H, J - 7.8 Hz), 7.35 (d, 1H, J = 8.0 Hz), 7.24-7.16 (m, 1H), 7.16-7.10 (m, 1H),
7.05 (d, 1H, J = 1.8 Hz), 3.19-3.12 (m, 1H), 2.96-2.72 (m, 4H), 2.49 (s, 1H), 2.36 (d, 1H, J
9.2 Hz), 2.09- 1.99 (m, 1H), 1.79- 1.71 (m, 1H), 1.71 - 1.65 (m, 1H), 1.63- 1.42 (m, 5H), 1.40- 1.31 (m, 1H), 1.24- 1.16 (m, 1H), 0.90 (t, 3H, J = 7.1 Hz) ppm; 13C NMR (CDC13, 100 MHz) (5 136.29, 127.83, 121.84, 121.65, 119.13, 119.04, 115.21, 111.15,56.89,56.82, 53.30, 40.62, 32.87, 27.44, 26.95, 25.35, 24.40, 21.97, 12.04 ppm; IR (Smart iTX Diamond) v3418, 3170, 3055,2930,2860, 2798, 1456, 1357, 1228, 1147, 1092, 740 cm1; LC-MS (ES+) calcd for C19H26N2 [M + H] 283.22 found 283.35.
Figure imgf000066_0001
[0209] Isolated as a tan solid (66 mg, 23%, 2 steps); ¾ NMR (CDC , 400 MHz) d 8.07 (s, 1H), 7.65 (d, 1H, J - 7.8 Hz), 7.35 (d, 1H, J = 8.0 Hz), 7.19 (t, 1H, J = 7.4 Hz), 7.12 (t, 1H, J = 7.4 Hz), 7.07-7.03 (m, 1H), 3.01-2.91 (m, 3H), 2.91-2.83 (m, 2H), 2.77-2.70 (m, 1H), 2.55-2.50 (m, 1H), 1.97-1.78 (m, 3H), 1.73-1.56 (m, 3H), 1.52-1.41 (m, 1H), 1.36-1.26 (m, 2H), 1.08-1.00 (m, 1H), 0.89 (t, 3H, J = 7.4 Hz) ppm; 13C NMR (CDCb, 100 MHz) d 136.37,
127.73, 122.03, 121.65, 119.30, 119.10, 114.97, 111.21,57.30, 56.20, 53.91,35.48, 32.92, 27.68, 26.67, 25.26, 24.39, 20.15, 12.19 ppm; IR (Smart iTX Diamond) v 3416, 3139, 3055, 2926, 2858, 2823, 1456, 1354, 1231, 1112, 1088, 738 cm1; LC-MS (ES+) calcd for C19H26N2 [M + H] 283.22 found 283.35. Example 8. Compounds 10a, and 10b
Figure imgf000066_0002
[0210] Isolated as an amorphous brown solid (71.2 mg, 44%, 2 steps); ¾ NMR (CDCb, 400 MHz) d 8.05 (s, 1H), 7.23 (d, 1H, J = 8.7 Hz), 7.09 (d, 1H, J = 2.0 Hz), 7.04-6.98 (m, 1H), 6.86 (dd, 1H, J = 8.8, 2.3 Hz), 3.88 (s, 3H), 3.27-3.12 (m, 1H), 2.96-2.76 (m, 4H), 2.54 (s, 1H), 2.37 (d, 1H, J = 9.3 Hz), 2.11 - 1.98 (m, 1H), 1.81-1.72 (m, 1H), 1.72-1.65 (m, 1H),
1.65-1.43 (m, 5H), 1.42-1.33 (m, 1H), 1.28-1.16 (m, 1H), 0.91 (t, 3H, J = 7.1 Hz) ppm; 13C NMR (CDCb, 100 MHz) d 153.91, 131.50, 128.16, 122.60, 114.58, 111.97, 111.87, 101.05, 56.85, 56.57, 56.13, 53.35, 40.43, 32.66, 27.38, 26.78, 25.15, 24.20, 21.90, 12.03 ppm; IR (Smart iTX Diamond) v 3415, 3045,2931,2860, 2828, 1624, 1585, 1485, 1456, 1215, 1172, 1064, 1032, 796 cm1; LC-MS (ES+) calcd for C20H29N2O [M + H] 313.23 found 313.34.
Figure imgf000067_0001
[0211] Isolated as an amorphous brown solid (56 mg, 18%, 2 steps); !H NMR (CDCh, 400 MHz) d 8.67 (s, 1H), 7.23 (d, 1H, J = 8.8 Hz), 7.08 (d, 1H, J = 2.3 Hz), 7.00 (d, 1H, J = 1.9 Hz), 6.86 (dd, 1H, J = 8.8, 2.4 Hz), 3.85 (s, 3H), 3.02-2.93 (m, 3H), 2.93 -2.86 (m, 2H), 2.80- 2.73 (m, 1H), 2.63 -2.56 (m, 1H), 2.02- 1.83 (m, 3H), 1.75- 1.59 (m, 3H), 1.55 - 1.44 (m, 1H),
1.40- 1.29 (m, 2H), 1.07 (m, 1H), 0.92 (t, 3H, J = 7.4 Hz) ppm; 13C NMR (CDCI3, 100 MHz) d 153.77, 131.57, 127.99, 122.66, 1 14.24, 1 14.21, 1 1 1.94, 100.83, 57.10, 56.10, 55.99, 53.63, 35.34, 32.87, 27.58, 26.60, 25.23, 24.32, 20.10, 12.14 ppm; IR (Smart iTX Diamond) v 3412, 3041, 2930, 2871, 2827, 1625, 1586, 1486, 1456, 1216, 1 173, 1064, 1032, 795 cm4; LC-MS (ES+) calcd for C20H29N2O [M + H] 313.23 found 313.34.
Example 9. Compound 11a and lib
[0212] First, the 5-benzyloxy-isoquinuclidine was synthesized as described above. Next, this starting material (100 mg, 0.26 mmol, 1.0 equiv) was dissolved MeOH (1.3 mL, 0.2 M) containing 10% Pd/C (22 mg, 0.02 mmol, 0.08 equiv) under a N2 atmosphere. The flask was then purged with ¾ gas and stirred vigorously under an atmosphere of ¾ until the starting material had been consumed as determined by TLC (~24 h). The mixture was filtered over a pad of celite, which was washed with 50 mL of MeOH containing 1% aqueous NH4OH. The filtrate was then dried over Na2S04 and concentrated under reduced pressure to yield a brown oil. The product was purified by chromatography on silica gel (30: 1 DCM:MeOH with 1% NH4OH)
Figure imgf000067_0002
[0213] Isolated as a tan foam (32 mg, 41%, 3 steps); ¾ NMR (CDCh, 400 MHz) d 7.96 (s, 1H), 7.14 (d, 1H, J - 8.6 Hz), 6.94 (s, 1H), 6.83 (d, 1H, J = 1.8 Hz), 6.80-6.72 (dd, 1H, J = 8.6, 1.8 Hz), 5.74 (bs, 1H), 3.13 (dt, 1H, J = 9.2, 2.9 Hz), 2.93 -2.71 (m, 4H), 2.56-2.49 (m, 1H), 2.35 (d, 1H, J = 9.5 Hz), 2.08 - 1.95 (m, 1H), 1.80- 1.71 (m, 1H), 1.70- 1.64 (m, 1H),
1.63 - 1.40 (m, 5H), 1.39- 1.17 (m, 2H), 0.88 (t, 3H, J = 7.1 Hz) ppm; 13C NMR (CDCh, 100 MHz) d 149.82, 131.40, 128.36, 122.85, 1 14.05, 1 12.26, 1 1 1.91, 103.71, 56.99, 56.59, 53.67, 40.64, 32.59, 27.43, 26.66, 25.01, 23.95, 22.08, 12.17 ppm; IR (Smart iTX Diamond) v 3400, 3278, 2929, 2860, 1625, 1581, 1456, 1362, 1 186, 1092, 936, 796, 754 cm 1; LC-MS (ES+) calcd for C19H27N2O [M + H] 299.21 found 299.34.
Figure imgf000068_0001
[0214] Isolated as a tan foam (20 mg, 15%, 3 steps); ¾ NMR (CDC , 600 MHz) d 7.98 (s,
1H), 7.16 (d, 1H, J = 8.6 Hz), 6.94 (s, 1H), 6.88 (d, 1H, J = 2.0 Hz), 6.78 (dd, 1H, J = 8.6, 2.2 Hz), 3.03 -2.92 (m, 4H), 2.76-2.71 (m, 1H), 2.62-2.57 (m, 1H), 2.01 - 1.82 (m, 3H), 1.69 (s, 1H), 1.67- 1.57 (m, 2H), 1.48- 1.41 (m, 1H), 1.35- 1.19 (m, 3H), 1.05-0.99 (m, 1H), 0.83 (t, 3H, J = 7.4 Hz) ppm; 13C NMR (CDCb, 150 MHz) d 150.66, 131.34, 128.18, 122.68, 1 13.51, 1 13.03, 1 12.03, 103.77, 57.33, 55.57, 54.52, 34.85, 32.65, 27.52, 26.35, 24.90, 24.01, 19.49,
12.09 ppm; IR (Smart iTX Diamond) v 3402, 3286, 2924, 2857, 1625, 1581, 1456, 1377, 1208, 1080, 936, 795, 751 cm 1; LC-MS (ES+) calcd for C19H27N2O [M + H] 299.21 found 299.34.
Example 10. Compound 12 [0215] To a solution of 4-substituted phenyl hydrazine hydrochloride (1.0 mmol) in EtOH
(0.1 M) was added 1 -methylazepan-4-one hydrochloride (164 mg, 1.0 mmol, 1.0 equiv) followed by concentrated aqueous HC1 (0.5 mL, 6.0 mmol, 6.0 equiv). The mixture was refluxed for 24h and then concentrated under reduced pressure. The oily residue was dissolved in DCM (~25mL) and basified with 1M aqueous NaOH (~20mL). The aqueous layer was extracted with DCM (3 x 20 mL). The combined organic extracts were dried over Na2S04 and concentrated under reduced pressure to yield an oil that was purified by chromatography on silica gel (20: 1 DCM:MeOH with 0.5% NH4OH).
General Scheme:
Figure imgf000068_0002
[0216] Isolated as a brown solid (127 mg, 64%); !H NMR (CDCb, 400 MHz) d 7.78 (s, 1H), 7.50-7.43 (m, 1H), 7.28 (d, 1H , J = 1.6 Hz), 7.15-7.05 (m, 2H), 3.01-2.92 (m, 4H), 2.92-2.82 (m, 4H), 2.53 (s, 3H) ppm; 13C NMR (CDCb-,,, 100 MHz) d 136.10, 134.68, 129.05, 121.12, 1 19.36, 117.74, 1 12.89, 1 10.44, 58.05, 56.30, 46.16, 28.59, 23.95 ppm; IR (Smart iTX Diamond) v 3140, 3055, 3032, 2904, 2829, 2756, 1451, 1337, 739 cm4; FC-MS (ES+) calcd for C13H17N2 [M + H] 201.14 found 201.33.
Example 11. Compound 13
Figure imgf000069_0001
[0217] Isolated as a light brown solid (146 mg, 63%; !H NMR (CDCb, 400 MHz) d 7.74 (s, 1H), 7.15 (d, 1H, J = 8.7 Hz), 6.92 (d, 1H, J = 2.4 Hz), 6.77 (dd, 1H, J = 8.7, 2.4 Hz), 3.85
(s, 3H), 2.98-2.82 (m, 8H), 2.53 (s, 3H) ppm; 13C NMR (100 MHz, CDCh) d 154.13, 137.12, 129.79, 129.40, 1 12.70, 11 1.15, 1 10.91, 100.13, 58.04, 56.29, 56.1 1, 46.15, 28.61, 24.00 ppm. IR (Smart iTX Diamond) v 3136, 3035, 2924, 2882, 2822, 1594, 1451, 1215, 1 107, 1035, 837, 791 cm4; LC-MS (ES+) calcd for CM H19N2O [M + H] 231.15 found 231.32. Example 12. Compound 14
Figure imgf000069_0002
[0218] Synthesized from the /3-benzyloxyhydrazine followed by hydrogenolysis of the benzyl group. Following Fisher indole cyclization, the benzyloxy indole was added to a mixture of 10% Pd/C (0.08 equiv) in MeOH (4.6 mF) under an atmosphere of N2. The slurry was stirred at room temperature for 12 hours, filtered over a small pad of Celite, and rinsed with 50 mF of methanol and 1% ammonium hydroxide. The filtrates were concentrated under reduced pressure to a brown oil. The desired product was purified by chromatography on silica gel (30: 1 DCM:MeOH with 0.5% NH4OH) to yield a white crystalline solid (80 mg, 40%, two steps). ¾ NMR (DMSO-rfe, 400 MHz) d 10.32 (s, 1H), 8.46 (s, 1H), 6.99 (d, 1H, J = 8.5 Hz), 6.65 (d, 1H, J = 2.2 Hz), 6.47 (dd, 1H , J = 8.5, 2.2 Hz), 2.87-2.79 (m, 2H), 2.70-
2.68 (m, 6H), 2.39 (s, 3H) ppm; 13C NMR (DMSO-rfe, 100 MHz) d 150.18, 137.63, 129.39, 129.05, 1 1 1.02, 1 10.58, 110.00, 101.62, 57.95, 56.14, 45.80, 27.76, 23.70 ppm; IR (Smart iTX Diamond) v 3391, 3273, 3047, 2927, 1590, 1455, 1200, 1 1 1 1, 928, 840, 797, 735 cm4. FC-MS (ES+) calcd for C13H17N2O [M + H] 217.13 found 217.32. Example 13. Compound 15
Figure imgf000070_0001
[0219] Isolated as a yellow solid (97 mg, 88%); !H NMR (CDC , 400 MHz) d 7.79 (s,
1H), 7.16 (dd, lH, = 8.8, 4.4 Hz), 7.09 (dd, 1H, J = 9.77, 2.37 Hz), 6.84 (td, 1H, J = 9.0, 2.4 Hz), 3.01-2.92 (m, 2H), 2.91-2.80 (m, 6H), 2.52 (s, 3H) ppm. 13C NMR (CDCb, 100 MHz) d 158.01 (d, = 233.9 Hz), 138.24, 131.12, 129.50 (d, .7 = 9.5 Hz), 1 13.23 (d, = 4.6 Hz),
1 10.90 (d, J = 9.7 Hz), 109.11 (d, J = 26.3 Hz), 102.86 (d, J = 23.4 Hz), 57.95, 56.19, 46.21, 28.77, 24.09 ppm. IR (Smart iTX Diamond) v 3146, 3103, 3040, 2929, 2880, 2809, 2753, 1584, 1453, 1 169, 1 103, 932, 855, 794, 767, 749 cm4. LC-MS (ES+) calcd for CI3HI6FN2 [M + H] 219.13 found 219.31.
Example 14. Compound 16
Figure imgf000070_0002
[0220] Isolated as a yellow solid (89 mg, 62%); !H NMR (CDCb, 400 MHz) d 7.99 (s, 1H), 7.41 (d, lH, = 1.7 Hz), 7.14 (d, 1H, = 8.5 Hz), 7.04 (dd, 1H, .7 = 8.5, 1.9 Hz), 2.98- 2.79 (m, 8H), 2.52 (s, 3H) ppm; 13C NMR (CDCI3, 100 MHz) d 137.82, 133.01, 130.16, 125.00, 121.13, 1 17.33, 112.68, 1 1 1.37, 57.84, 56.08, 46.04, 28.55, 23.85 ppm; IR (Smart iTX Diamond) v 3128, 3085, 3020, 2922, 2849, 2736, 2694, 1446, 1361, 1316, 1052, 917, 851, 783, 718, 601 cm . LC-MS (ES+) calcd for C13H16CIN2 [M + H] 235.10 found 235.30.
Example 15. Compound 17
Figure imgf000070_0003
[0221] Isolated as a brown solid (161 mg, 65%); !H NMR (CDCb, 400 MHz) d 7.93 (s, 1H), 7.57 (d, 1H, J = 1.24 Hz), 7.17 (dd, 1H, .7 = 8.5, 1.5 Hz), 7.10 (d, 1H, = 8.5 Hz), 2.99- 2.77 (m, 8H), 2.52 (s, 3H) ppm; 13C NMR (CDCb, 100 MHz) d 137.66, 133.27, 130.84, 123.71, 120.45, 1 12.66, 112.58, 1 1 1.82, 57.86, 56.1 1, 46.12, 28.62, 23.91 ppm; IR (Smart iTX Diamond) v 3126, 3082, 3020, 2922, 2848, 2733, 1575, 1450, 1316, 1 1 13, 1046, 914, 852, 781, 739, 594 cm4. LC-MS (ES+) calcd for Ci3Hi6BrN2 [M + H] 279.05 found 279.22. Example 16. Compound 18
[0222] Compound 18 (TBG), was prepared analogously to compounds 12-17 with slight modifications.
General Scheme:
Figure imgf000071_0001
[0223] To an ice-cold solution of aqueous 6 M HC1 (15 mL) was added 3-methoxyaniline (2.2 ml, 20.0 mmol, 1.0 equiv) dropwise. Next, NaNO2 (1.520 g, 22.0 mmol, 1.1 equiv) was dissolved in ¾0 (15 mL) and added to the solution slowly. After stirring at 0 °C for 15 minutes, SnCL (11.4 g, 60.0 mmol, 3.0 equiv) dissolved in concentrated aqueous HC1 (15 mL) was added to the solution dropwise. After stirring at 0 °C for 2.5 h, the reaction mixture was filtered, washed with hexanes, and dried under reduced pressure to yield the product as a light yellow solid that was used without further purification.
[0224] To a solution of 3-methoxyphenylhydrazine hydrochloride (1.566 g, 9.0 mmol, 3.0 equiv) in 0.1 M EtOH (30 mL) was added 1 -methylazepan-4-one hydrochloride (489 mg, 3.0 mmol, 1.0 equiv) followed by concentrated aqueous HC1 (1.0 mL, 12.0 mmol, 4.0 equiv). The mixture was refluxed for 12 h and then concentrated under reduced
pressure. The oily residue was dissolved in DCM (~25mL) and basified with 1M aqueous NaOH (~20mL). The aqueous layer was extracted with DCM (3 x 20 mL). The combined organic extracts were dried over Na2S04 and concentrated under reduced pressure to yield a mixture of the 6- and 4-substituted indoles. The 6-substituted indole was purified by chromatography on silica gel (10: 1 DCM:MeOH with 0.5% NH4OH).
Figure imgf000071_0002
[0225] Isolated as light yellow solid (379 mg, 55%); !H NMR (CDCL, 400 MHz) d 7.67 (br s, 1H), 7.32 (d, 1H , J= 8.5 Hz), 6.80 - 6.73 (m, 2H), 3.83 (s, 3H), 2.98 - 2.83 (m, 8H), 2.53 (s, 3H) ppm; 13C NMR (CDCI3, 100 MHz) d 155.93, 135.33, 134.68, 123.59, 118.29,
112.53, 108.80, 94.66, 57.98, 56.34, 55.95, 45.93, 28.36, 23.91 ppm; IR (Smart iTX
Diamond) v 3153, 3121, 3073, 2940, 2879, 1628, 1458, 1197, 1033, 910, 834, 799 cm 1; LC- MS (ES+) calcd for C14H19N2O [M+H] 231.15 found 231.36. Example 17. Procedure for Preparing Compound 13 ½ fumarate
[0226] Fumaric acid (116 mg, 1.0 mmol, 0.8 equiv) was added to a sealed tube containing acetone (12 mL). The solution was carefully heated until all of the fumaric acid dissolved. After cooling the solution to room temperature, a solution of 13 free base (288 mg, 1.25 mmol, 1.0 equiv) in acetone (1 mL) was added, and the mixture was cooled in the freezer overnight. The solid was filtered, washed with acetone, and dried under reduced pressure to yield 13 fumarate as the 2: 1 salt (220 mg, 61%); !H NMR (CD3OD, 400 MHz) d 7.15 (d, 1H , J = 8.7 Hz), 6.92 (s, 1H), 6.72 (d, 1H , J = 8.7 Hz), 6.68 (s, 1H), 3.80 (s, 3H), 3.42-3.34 (m, 4H), 3.18 (t, 2H, J= 5.5 Hz), 3.10 (t, 2H , J= 5.5 Hz), 2.91 (s, 3H) ppm; 13C NMR (CD3OD, 100 MHz) d 171.76, 155.37, 136.32, 135.55, 131.75, 129.59, 112.49, 1 12.31,
1 10.58, 100.58, 58.77, 56.70, 56.27, 44.77, 25.09, 21.55 ppm. IR (Smart iTX Diamond) v 3384, 2979, 2924, 2297, 2822, 1695, 1551, 1353 1167, 982, 912, 802 cm4; LC-MS (ES+) calcd for C14H19N2O [M+H] 231.15 found 231.36.
Example 18. Procedure for Preparing Compound 18 fumarate
[0227] Fumaric acid (77 mg, 0.66 mmol, 0.8 equiv) was added to a sealed tube containing acetone (8 mL). The solution was carefully heated until all of the fumaric acid dissolved. After cooling the solution to room temperature, a solution of 18 free base (193 mg, 0.84 mmol, 1.0 equiv) in acetone (1 mL) was added, and the mixture was cooled in the freezer overnight. The solid was filtered, washed with acetone, and dried under reduced pressure to yield 18 fumarate as the 1 : 1 salt (187 mg, 64%). !H NMR (CD3OD, 400 MHz) d 7.28 (d, 1H , J = 8.7 Hz), 6.82 (s, 1H), 6.71 (s, 2H), 6.68 (d, 1H, J= 8.7 Hz), 3.79 (s, 3H), 3.54-3.45 (m, 4H), 3.22 (t, 2H, J= 5.3 Hz), 3.13 (t, 2H , J= 5.3 Hz), 3.00 (s, 3H) ppm; 13C NMR (CD3OD, 100 MHz) d 170.71, 157.57, 137.23, 135.99, 133.23, 123.71, 118.86, 1 10.47,
1 10.10, 95.31, 58.62, 56.68, 55.99, 44.48, 24.77, 21.35 ppm; IR (Smart iTX Diamond) v 3375, 3008, 2899, 2505, 1694, 1556, 1337, 1 162, 1034, 967, 814, 775 cm 1; LC-MS (ES+) calcd for C14H19N2O [M+H] 231.15 found 231.36.
Example 19. Procedure for the Large Scale Preparation of Compound 18 fumarate
[0228] To an ice-cold solution of aqueous 6 M HC1 (30 mL) was added 3-methoxyaniline (4.4 ml, 40.0 mmol, 1.0 equiv) dropwise. Next, NaNC (3.040 g, 44.0 mmol, 1.1 equiv) was dissolved in H2O (30 mL) and added to the solution slowly. After stirring at 0 °C for 15 minutes, SnCL (22.8 g, 120.0 mmol, 3.0 equiv) dissolved in concentrated aqueous HC1 (30 mL) was added to the solution dropwise. After stirring at 0 °C for 2.5 h, the reaction mixture was filtered, washed with hexanes, and dried under reduced pressure to yield 3- methoxyphenylhydrazine hydrochloride as a light yellow solid (5.4 g, 78%) that was used without further purification. [0229] To a solution of 3-methoxyphenylhydrazine hydrochloride (4.802 g, 27.6 mmol, 3.0 equiv) in 0.15 M EtOH (60 mL) was added l-methylazepan-4-one hydrochloride (1.5 g, 9.2 mmol, 1.0 equiv) followed by concentrated aqueous HC1 (3.1 mL, 36.8 mmol, 4.0 equiv).
The mixture was refluxed for 12 h and then concentrated under reduced pressure. The oily residue was dissolved in DCM (~50 mL) and basified with 1M aqueous NaOH (~50 mL). The aqueous layer was extracted with DCM (3 x 30 mL). The combined organic extracts were dried over Na2S04 and concentrated under reduced pressure to yield a mixture of the 6- and 4-substituted indoles. Addition of EtOAc to the unpurified product mixture resulted in precipitation of the 6-substituted isomer, which was isolated via filtration. The mother liquor was concentrated, and this procedure was repeated six times until a total of 1.028 g of tabemanthalog free base (49%) was collected.
[0230] Next, fumaric acid (408 mg, 3.5 mmol, 0.8 equiv) was added to a sealed tube containing acetone (20 mL). The solution was carefully heated until all of the fumaric acid dissolved. After cooling the solution to room temperature, a solution of 18 free base (1.028 mg, 4.4 mmol, 1.0 equiv) in acetone (5 mL) was added dropwise, and the mixture was cooled in the freezer overnight. The solid was filtered, washed with acetone, and dried under reduced pressure to yield 18 fumarate as the 1 : 1 salt (1.055 g, 69%).
Example 20. General Procedure for Examples 21 to 24
General synthetic scheme:
Figure imgf000073_0001
General procedure:
[0231] To a solution of substituted phenyl hydrazine hydrochloride (1.0 mmol) in EtOH (0.1 M) was added 1 -methylazepan-4-one hydrochloride (1.0 equiv) followed by concentrated aqueous HC1 (6.0 equiv). The mixture was refluxed for 24h and the progress of the reaction is monitored by TLC.
[0232] Work up and purification Procedure: after completion of the reaction, the reaction mixture was concentrated under reduced pressure. The oily residue was dissolved in DCM (~25mL) and basified with 1M aqueous NaOH (~20mL). The aqueous layer was extracted with DCM (3 x 20 mL). The combined organic extracts were dried over Na2S04 and concentrated under reduced pressure to yield an oil that was purified by combi-flash using 0.5% NH4OH with varying % of MeOH in CH2CI2. The cleaner fractions by TLC were evaporated and then the obtained residue was diluted with EtOAc and washed with water for couple of times. The organic layer was separated and then evaporated and dried to get pure product.
[0233] A few compounds are purified by prep-HPLC purification and the purification methods are captured at respective target compounds.
Example 21. Compound 19
Figure imgf000074_0001
[0234] Yield: 150 mg (8%, Off white solid). LCMS: 99%, m/z=217.1 [M+H]+ ¾ NMR (DMSO-ί/ή, 400 MHz): d 10.42 (s, 1H), 7.20 (d, J= 8.56 Hz, 1H), 6.72 (d, J= 2.20 Hz, 1H),
6.57 (dd, J= 2.20, 8.56 Hz, 1H), 3.72 (s, 3H), 2.87-2.91 (m, 4H), 2.79-2.82 (m, 2H), 2.68-2.72 (m, 2H).
Example 22. Compound 20 & Compound 21
Figure imgf000074_0002
[0235] The crude was purified by prep-HPLC to get fraction- 1 and fraction-2. Fraction-2 is the above left compound by 11 1 NMR whereas fraction- 1 is the above right compound.
[0236] Prep-HPLC purification method:
Preparative HPLC Column : Viridis BEH-2 EP OBD (250* 19mm, 5m)
Mobile Phase A : 0.1% DEA in n- Hexane
Mobile Phase B : EtOH: MeOH ( 50:50)
Flow rate : 16.0 mL/min
Isocratic Table :
Figure imgf000075_0002
Solvents used for dilution : Methanol/Ethanol
[0237] Compound 20: Yield: 12.8% (80 mg , pale brown solid). LCMS: 99.2%, m/z=285.2 [M+Hf *H NMR (DMSO- , 400 MHz): d 10.97 (s, 1H), 7.43 (d, J=8.56 Hz,
1H), 7.18 (d, J=0.86 Hz, 1H), 6.87-6.92 (m, 1H), 2.88-2.93 (m, 2H), 2.72-2.83 (m, 6H), 2.41 (s, 3H). [0238] Compound 21: Yield: 1.6% (10 mg, Off white solid). LC-MS: 99.6%, m/z=285.2
[M+Hf ¾ NMR (DMSO- , 400 MHz): d 1 1.20 (br s, 1H), 7.27 (d, J=8.07 Hz, 1H), 7.02 (t, J= 7.95 Hz, 1H), 6.87 (br d, J= 7.09 Hz, 1H), 2.97-3.00 (m, 2H), 2.91-2.94 (m, 2H), 2.69-2.73 (m, 4H), 2.40 (s, 3H).
Example 23. Compound 22
Figure imgf000075_0001
[0239] Prep-HPLC purification method:
Preparative HPLC Column : X-select CSH C18 (250* 19mm), 5um
Mobile Phase A : Acetonitrile
Mobile Phase B : 0.05% Ammonia in H20
Flow rate : 15.0 mL/min
Gradient Table
Figure imgf000076_0003
Solvents used for dilution : Acetonitrile/MeOH
[0240] Yield: 15.6% (30 mg, Off white solid). LCMS: 99.6%, m/z=261.1 [M+Hf *H NMR (DMSO-ί/ή, 400 MHz): d 10.32 (s, 1H), 6.87 (s, 1H), 6.76 (s, 1H), 3.73 (d, J= 1.59 Hz, 6H), 2.81-2.85 (m, 2H), 2.68-2.74 (m, 6H), 2.39 (s, 3H).
Example 24. Compound 23
Figure imgf000076_0001
23
[0241] Yield: 220 mg (34%, Pale brown solid). LCMS: 100%, m/z=245.1 [M+H]+ 11 1 NMR (DMSO-ί/ή, 400 MHz): d 10.45 (br s, 1H), 6.84 (s, 1H), 6.75 (s, 1H), 5.86 (s, 2H), 2.79- 2.82 (m, 2H), 2.68 (br s, 6H), 2.37 (s, 3H).
Example 25. Compound 24 & Compound 25
Figure imgf000076_0002
[0242] The crude was purified by prep-HPLC to get fraction- 1 and If action-2. The fraction- 1 is # (left) by 11 1 NMR whereas fraction-2 is # (right).
[0243] Prep-HPLC purification method:
Preparative HPLC Column : Viridis BEH-2 EP OBD (250* 19mm, 5m)
Mobile Phase A : 0.1 % DEA in n- Hexane Mobile Phase B : EtOH: MeOH ( 50:50)
Flow rate : 16.0 mL/min
Isocratic Table :
Figure imgf000077_0002
Solvents used for dilution : Methanol/Ethanol
[0244] Compound 24: Yield: 9.1% (80 mg , off white solid). LCMS: 98.7%, m/z=215.2 [M+Hf *H NMR (DMSO- , 400 MHz): d 10.47 (s, 1H), 7.22 (d, J=7.95 Hz, 1H), 7.00 (s, 1H), 6.74 (dd, J= 0.98, 7.95 Hz, 1H), 2.84-2.88 (m, 2H), 2.70-2.76 (m, 6H), 2.40 (s, 3H), 2.35 (s, 3H). [0245] Compound 25: Yield: 3.4% (30 mg, Off white solid). LC-MS: 95.8%, m/z=215.2
[M+Hf *H NMR (DMSO-ώ.400 MHz): d 10.68 (br s, 1H), 7.05 (d, J=7.95 Hz, 1H), 6.82 (t, J= 7.52 Hz, 1H), 6.62 (d, J=7.09 Hz, 1H), 3.10-3.14 (m, 2H), 2.89-2.92 (m, 2H), 2.81 (br s, 4H), 2.56 (s, 3H), 2.46 (br s, 3H).
Example 26. Compound 26 & Compound 27
Figure imgf000077_0001
[0246] The crude was purified by prep-HPLC to get fraction- 1 and If action-2. The ffaction- 1 is # (right) by 11 1 NMR whereas fraction-2 is # (left).
[0247] Prep-HPLC purification method: Preparative HPLC Column : Chiralpak IC (250*30mm,5p)
Mobile Phase A : 0.1% DEA in n- Hexane
Mobile Phase B : EtOH: MeOH (80:20)
Flow rate : 35.0 mL/min
Isocratic Table
Figure imgf000078_0002
Solvents used for dilution : Methanol/Ethanol
[0248] Compound 26: Yield: 24.6% (160 mg , off white solid). LCMS: 100%, m/z=245.1 [M+Hf *H NMR (DMSO- , 400 MHz): d 10.33 (s, 1H), 7.07 (s, 1H), 6.71 (s, 1H), 3.75 (s, 3H), 2.81-2.85 (m, 2H), 2.69-2.73 (m, 6H), 2.39 (s, 3H), 2.18 (s, 3H).
[0249] Compound 27: Yield: 3.1% (20 mg, Off white solid). LC-MS: 100%, m/z=245.1 [M+Hf ¾ NMR (DMSO-ώ, 400 MHz): d 10.63 (s, 1H), 6.89 (d, J=8.07 Hz, 1H), 6.75 (d, J= 8.07 Hz, 1H), 3.65 (s, 3H), 3.03-3.06 (m, 2H), 2.82-2.85 (m, 2H), 2.64-2.67 (m, 4H), 2.37 (s, 3H), 2.23 (s, 3H). Example 27. Compound 28
Figure imgf000078_0001
[0250] Compound 28: Yield: 4.7% (30 mg, pale brown solid). LC-MS: 96.2%, m/z=261.2 [M+Hf *H NMR (DMSO- , 400 MHz): d 10.44 (s, 1H), 6.32 (d, JM .96 Hz, 1H), 6.05 (d, JM .96 Hz, 1H), 3.76 (s, 3H), 3.71 (s, 3H), 3.02-3.04 (m, 2H), 2.77-2.80 (m, 2H), 2.64 (br dd, J=3.24, 6.17 Hz, 4H), 2.35 (s, 3H).
Plasticity Effects.
[0251] Treatment of rat embryonic cortical neurons with TBG led to increased dendritic arbor complexity at 6 days in vitro (DIV6) as measured by Sholl analysis (FIG. 3A-C). The effect of TBG on dendritic growth appears to be 5-HT2A-dependent, as pretreatment with ketanserin— a 5-HT2A antagonist— completely abrogated its effects (FIG. 3D), which further validates in vitro receptor profiling.
[0252] In addition to promoting dendritic growth, TBG also increases dendritic spine density to a comparable extent as ibogaine in mature cortical cultures (DIV20) (FIG. 3E and FIG. 3F). The effects of TBG on cortical dendritic spine dynamics in vivo using transcranial 2-photon imaging (FIG. 3G) was assessed. First, spines were imaged on specific dendritic loci defined by their relation to blood vessel and dendritic architectures. Next, the animals were systemically administered vehicle, TBG, or the hallucinogenic 5-HT2A agonist 2,5- dimethoxy-4-iodoamphetamine (DOI). After 24 h, the same dendritic segments were re imaged, and the number of spines gained or lost was quantified. Neither TBG nor DOI affected spine elimination. Both compounds increased spine formation in mouse primary sensory cortex (FIG. 3H and FIG. 31).
[0253] As increased cortical structural plasticity in the anterior parts of the brain mediates the sustained (>24 h) antidepressant- like effects of ketamine and play a role in the therapeutic effects of 5-HT2A agonists, the impact of TBG on forced swim test (FST) behavior (FIG. 4A and FIG. 4B) was evaluated. First, a pretest was used to induce a depressive phenotype. Drugs were administered 24 h after the pre-test, and the FST was performed 24 h and 7 d post drug administration. Both ketamine and TBG significantly reduced immobility 24 h after drug administration.
[0254] To assess the anti-addictive potential of TBG, an alcohol drinking paradigm that models heavy alcohol use and binge drinking behavior in humans was employed. Using a 2- bottle choice setup (20% ethanol (v/v), EtOFI vs. water, FEO), mice were subjected to repeated cycles of binge drinking and withdrawal over the course of 7 weeks (FIG. 4C).
This schedule results in heavy EtOH consumption (11.44 ± 0.76 g/kg/24h), binge drinking like behavior (3.89 ± 0.33 g/kg/4h), and generates blood alcohol content equivalent to that of human subjects suffering from alcohol use disorder (AUD). Next, TBG or vehicle were administered via intraperitoneal injection 3 h prior to a drinking session, and EtOH and ¾0 consumption was monitored (FIG. 4C). As shown in FIG. 4D, TBG robustly reduces binge drinking during the first 4 h with every animal (19 total) decreasing EtOH consumption. Water intake was not affected (FIG. 4D). Consumption of ethanol was lower for at least two days following TBG administration with no effect on water intake (FIG. 4E). Similar effects have been observed previously for ibogaine suggesting that TBG may be a highly efficacious agent for the treatment of AUD.
Biological Protocols.
[0255] Data Analysis and Statistics. Treatments were randomized, and data were analyzed by experimenters blinded to treatment conditions. Statistical analyses were performed using GraphPad Prism (version 8.1.2) unless noted otherwise. All comparisons were planned prior to performing each experiment. Data are represented as mean ± SEM, unless otherwise noted, with asterisks indicating *p < 0.05, **p < 0.01 , ***p < 0.001, and ****p < 0.0001. Boxplots depict the three quartile values of the distribution with whiskers extending to points that lie within 1.5 IQRs (interquartile range) of the lower and upper quartile. Observations falling outside this range are displayed independently. For FIGs. 2B, 3C, 3D, 3F, 31, 4B and 5B, compound treatments were compared to the VEH control using a one-way ANOVA with Dunnett’s post hoc test. For FIGs. 2E and F, time 0 h and time 1 h (before and after drug administration, respectively) were compared using a paired t-test. For FIGs. 21 and 11, compound treatments were compared using Fisher’s exact test and p-values are indicated in the text. For FIG. 4D, data were analyzed using a paired t-test. FIG. 4E, data were analyzed using a two-way ANOVA with Sidak’s post hoc test.
[0256] Drugs. The NIFI Drug Supply Program provided ibogaine hydrochloride (IBO) and noribogaine (NOR). Other chemicals were purchased from commercial sources such as ketamine hydrochloride (KET, Fagron), ketanserin (KETSN, ApexBio), eugenol (Tokyo Chemical Industries), and 5-hydroxytryptamine (Sigma-Aldrich). The fumarate salt of 5- m e t h o x y - /V, /V- d i m e t h y 11 ry p t a m i n e (2: 1, 5-MeO-DMT:fumaric acid) was synthesized in house as described previously and judged to be analytically pure based on NMR and LC-MS data. For cell culture experiments, VEFI = 0.1% (agonist studies) or 0.2% (antagonist studies) molecular biology grade dimethyl sulfoxide (Sigma-Aldrich). For in vivo experiments, VEH = USP grade saline (0.9%). Free bases were used for all cellular experiments while the fumarate salts of ibogainalog and tabemanthalog were used for the in vivo studies.
[0257] Animals. All experimental procedures involving animals were approved by either the UCD, UCSF, or UCSC Institutional Animal Care and Use Committee (IACUC) and adhered to principles described in the National Institutes of Health Guide for the Care and Use of Laboratory Animals. The University of California, Davis (UCD), the University of California, San Francisco (UCSF), and the University of California, Santa Cruz (UCSC) are accredited by the Association for Assessment and Accreditation of Laboratory Animal Care International (AAALAC).
[0258] Calculation of CNS MPO Score. CNS MPO scores were calculated using a previously published method. Predicted PKA values were determined using Marvin Sketch (19.25.0). LogP and total polar surface area were predicted using Molinspiration. LogD was calculated using the following equation LogD = LogP - LOGio(l+10(pka-7 4)). [0259] Dendritogenesis Experiments. For the dendritogenesis experiments conducted using cultured cortical neurons, timed pregnant Sprague Dawley rats were obtained from Charles River Laboratories (Wilmington, MA). Full culturing, staining, and analysis details were performed as previously described. (Dunlap, L. et al., 2019)
[0260] Head-Twitch Response (HTR). The head-twitch response assay was performed as described previously using both male and female C57BL/6J mice (2 per treatment). The mice were obtained from Jackson Laboratory (Sacramento, C.A.) and were approximately 8 weeks old at the time of the experiments. Compounds were administered via intraperitoneal injection (5 mL/kg) using 0.9% saline as the vehicle. As a positive control, 5-MeO-DMT fumarate (2:1 amine/acid) was utilized, which was synthesized as described previously. Behavior was videotaped, later scored by two blinded observers, and the results were averaged (Pearson correlation coefficient = 0.93).
[0261] hERG Inhibition Studies. All experiments were conducted manually using an EPC-10 amplifier (HEKA, Lambrecht/Pfalz, Germany) at room temperature in the whole-cell mode of the patch-clamp technique. HEK-293 cells stably expressing hKvl 1.1 (hERG) under G418 selection were a generous gift from Craig January (University of Wisconsin, Madison). Cells were cultured in DMEM containing 10% fetal bovine serum, 2 mM glutamine, 1 mM sodium pyruvate, 100 U/mL penicillin, 100 pg/mL streptomycin, and 500 mg/ml G418. Before experiments, cells were cultured to 60-80% confluency and lifted using TrypLE and plated onto poly-L-lysine-coated coverslips. Patch pipettes were pulled from soda lime glass (micro-hematocrit tubes) and had resistances of 2-4 MW. For the external solution, normal sodium Ringer was used (160 mM NaCl, 4.5 mM KC1, 2 mM CaCh, 1 mM MgCk, 10 mM HEPES, pH 7.4 and 290-310 mOsm). The internal solution used was potassium fluoride with ATP (160 mM KF, 2 mM MgCL, 10 mM EGTA, 10 mM HEPES, 4 mM NaATP, pH = 7.2 and 300-320 mOsm). A 2-step pulse (applied every 10 sec) from -80 mV first to 40 mV for 2 sec and then to -60 mV for 4 sec, was used to elicit hERG currents. The percent reduction of tail current amplitude by the drugs was determined and data are shown as mean +/- SD. For all experiments, solutions of the drugs were prepared fresh from 10 mM stock solutions in DMSO. The final DMSO concentration never exceeded 1%.
[0262] Serotonin and Opioid Receptor Functional Assays. Functional assay screens at 5- HT and opioid receptors were performed in parallel using the same compound dilutions and 384-well format high-throughput assay platforms. Assays assessed activity at all human isoforms of the receptors, except where noted for the mouse 5-HT2A receptor. Receptor constructs in pcDNA vectors were generated from the Presto-Tango GPCR library with minor modifications. All compounds were serially diluted in drug buffer (HBSS, 20 mM HEPES, pH 7.4 supplemented with 0.1% bovine serum albumin and 0.01% ascorbic acid) and dispensed into 384-well assay plates using a FLIPRTETRA (Molecular Devices). Every plate included a positive control such as 5-HT (for all 5-HT receptors), DADLE (DOR), salvinorin A (KOR), and DAMGO (MOR). For measurements of 5-HT2A, 5-HT2B, and 5- HT2C Gq-mediated calcium flux function, HEK Flp-In 293 T-Rex stable cell lines
(Invitrogen) were loaded with Fluo-4 dye for one hour, stimulated with compounds and read for baseline (0-10 seconds) and peak fold-over-basal fluorescence (5 minutes) at 25°C on the FLIPRTETRA. For measurement of 5-HT6 and 5-HT7a functional assays, Gs-mediated cAMP accumulation was detected using the split-luciferase GloSensor assay in HEKT cells measuring luminescence on a Microbeta Trilux (Perkin Elmer) with a 15 min drug incubation at 25°C. For 5-HT1A, 5-HT1B, 5-HT1F, MOR, KOR, and DOR functional assays, Gi/o- mediated cAMP inhibition was measured using the split-luciferase GloSensor assay in HEKT cells, conducted similarly as above, but in combination with either 0.3 mM isoproterenol (5- HT1A, 5-HT1B, 5-HT1F) or 1 pM forskolin (MOR, KOR, and DOR) to stimulate endogenous cAMP accumulation. For measurement of 5-HT1D, 5-HT1E, 5-HT4, and 5- HT5A functional assays, -arrestin2 recruitment was measured by the Tango assay utilizing HTLA cells expressing TEV fused- -arrestin2, as described previously with minor modifications. Data for all assays were plotted and non-linear regression was performed using“log(agonist) vs. response” in Graphpad Prism to yield Emax and EC50 parameter estimates.
[0263] 5HT2A Sensor Assays. HEK293T (ATCC) 5HT2A sensor stable line (sLightl .3s) was generated via lentiviral transduction of HIV-EFla-sLightl.3 and propagated from a single colony. Lentivirus was produced using 2nd generation lentiviral plasmids pHIV-EFl a - sLightl .3, pHCMV-G, and pCMV-deltaR8.2.
[0264] For the screening of the 41 compounds, sLightl .3s cells were plated in 96-well plates at a density of 40000 24-hours prior to imaging. On the day of imaging, compounds solubilized in DMSO were diluted from the 1 OOmM stock solution to working concentrations of ImM, IOOmM and lpM with a DMSO concentration of 1%. Immediately prior to imaging, cells growing in DMEM (Gibco) were washed 2x with HBSS (Gibco) and in agonist mode 180pL of HBSS or in antagonist mode 160pL of HBSS was added to each well after the final wash. For agonist mode, images were taken before and after the addition of the 20pL compound working solution into the wells containing 180pL HBSS. This produced final compound concentrations of 100mM, 1 OmM and IOOhM with a DMSO concentration of 0.1%. For antagonist mode, images were taken before and after addition of 20pL of 900nM 5-HT and again after 20pL of the compound working solutions to produce final concentrations of lOOnM for 5HT and 100mM, 10m M and lOOnM for the compounds with a DMSO
concentration of 0.1%. Each compound was tested in triplicates (3 wells) for each concentration (100mM, 10mM and lOOnM). Additionally, within each plate, lOOnM 5HT and 0.1% DMSO controls were also imaged.
[0265] Imaging was performed using the Leica DMi8 inverted microscope with a 40x objective using the FITC preset with an excitation of 460nm and emission of 512-542nm. For each well, the cellular membrane where the 5HT2A sensor is targeted was autofocused using the adaptive focus controls and 5 images from different regions within the well were taken with each image processed from a 2x2 binning.
[0266] For data processing, the membranes from each image was segmented and analyzed using a custom algorithm written in MATLAB producing a single raw fluorescence intensity value. For each well the 5 raw fluorescence intensity values generated from the 5 images were averaged and the change in fluorescence intensity (dFF) was calculated as: dFF = (Fsat - Fapo)/ Fapo
[0267] For both agonist and antagonist modes, the fluorescence intensity values before compound addition in FIBSS only were used as the Fapo values while the fluorescence intensity values after compound addition were used as the Fsat values.
[0268] For agonist mode, data are as percent activation relative to 5HT, where 0 is the average of the DMSO wells and 100 is the average of the 100 mM 5HT wells. For antagonist mode, the inactivation score was calculated as:
Inactivation score = (dFFF(Compound+5HT) - dFF(5HT))/dFF(5HT)
[0269] Spinogenesis Experiments. Spinogenesis experiments were performed as previously described with the exception that cells were treated on DIV 19 and fixed 24 h after treatment on DIV20. (Ly, C. et al., 2018) The images were taken on a Nikon HCA Confocal microscope a with a 100x/NA 1.45 oil objective. DMSO and ketamine (10 mM) were used as vehicle and positive controls, respectively. [0270] In Vivo Spine Dynamics. Male and female Thyl- GFP-M line mice (n = 5 per condition) were purchased from The Jackson Laboratory (JAX #007788) and maintained in UCSC animal facilities according to an IACUC approved protocol. In vivo transcranial two- photon imaging and data analysis were performed as previously described. Briefly, mice were anesthetized with an intraperitoneal (i.p.) injection of a mixture of ketamine (87 mg/kg) and xylazine (8.7 mg/kg). A small region of the exposed skull was manually thinned down to 20- 30 pm for optical access. Spines on apical dendrites in mouse primary sensory cortices were imaged using a Bruker Ultima IV two-photon microscope equipped with an Olympus water- immersion objective (40x, NA = 0.8) and a TLSapphire laser (Spectra-Physics Mai-Tai, excitation wavelength 920 nm). Images were taken at a zoom of 4.0 (pixel size 0.143 x 0.143 pm) and Z-step size of 0.7 pm. The mice received an i.p. injection (injection volume = 5 mL/kg) of DOI (10 mg/kg) or TBG (50 mg/kg) immediately after they recovered from anesthesia given prior to the first imaging session. The animals were re-imaged 24 h after drug administration. Dendritic spine dynamics were analyzed using ImageJ. Spine formation and elimination were quantified as percentages of spine number on day 0.
[0271] Forced Swim Test (FST). Male C57/BL6J mice (9-10 weeks old at time of experiment, n = 10 per condition) were obtained from the Jackson Lab and housed 4-5 mice/cage in a UCD vivarium following an IACUC approved protocol. After 1 week in the vivarium each mouse was handled for approximately 1 minute by a male experimenter for 3 consecutive days leading up to the first FST. All experiments were carried out by the same male experimenter who performed handling. During the FST, mice underwent a 6 min swim session in a clear Plexiglas cylinder 40 cm tall, 20 cm in diameter, and filled with 30 cm of 24 ± 1°C water. Fresh water was used for every mouse. After handling and habituation to the experimenter, drug-nai've mice first underwent a pretest swim to more reliably induce a depressive phenotype in the subsequent FST sessions. Immobility scores for all mice were determined after the pre-test and mice were randomly assigned to treatment groups to generate groups with similar average immobility scores to be used for the following two FST sessions. The next day, the animals received intraperitoneal injections of TBG (50 mg/kg), a positive control (ketamine, 3 mg/kg), or vehicle (saline). The following day, the animals were subjected to the FST and then returned to their home cages. One week later, the FST was performed again to assess the sustained effects of the drugs. All FSTs were performed between the hours of 8 am and 1 pm. Experiments were video-recorded and manually scored offline. Immobility time— defined as passive floating or remaining motionless with no activity other than that needed to keep the mouse’s head above water— was scored for the last 4 min of the 6 min trial.
[0272] Alcohol Consumption. Male C57/BL6J mice (6-8 weeks old) were obtained from The Jackson Laboratory (Bar Harbor, ME) and were individually housed in a reverse light/dark cycle room (lights on 10:00pm-10:00am). Temperature was kept constant at 22 ± 2°C, and relative humidity was maintained at 50 ± 5%. Mice were given access to food and tap water ad libitum. After one week of habituation to the vivarium, the two-bottle choice alcohol-drinking paradigm was conducted as described previously. For 7 weeks, mice were given intermittent access in their home cage to alcohol. On Mondays, Wednesdays, and Fridays, two bottles were made available for 24 h— one containing 20% ethanol and another containing only water. On Tuesdays, Thursdays, Saturdays, and Sundays, the animals were only given access to water. After 7 weeks, mice were administered TBG (50 mg/kg) or vehicle (saline) via intraperitoneal injection 3 h before the beginning of a drinking session. Ethanol (g/kg) and water (ml/kg) intake were monitored during the first 4 h (initial binge), the first 24 h, and the second 24 h. Next, the animals were only given water for 48 h before the start of another drinking session when ethanol and water consumption was monitored. The placement (right or left) of the bottles was altered in each session to control for side preference. Spillage was monitored using an additional bottle in a nearby unused cage. Alcohol preference was calculated as the ratio between alcohol/(water + alcohol). Mice were tested using a counterbalanced, within subject design with one week of drug-free alcohol drinking regimen between treatments. One mouse was excluded because the bottle was leaking.
[0273] Although the foregoing invention has been described in some detail by way of illustration and Example for purposes of clarity of understanding, one of skill in the art will appreciate that certain changes and modifications may be practiced within the scope of the appended claims. In addition, each reference provided herein is incorporated by reference in its entirety to the same extent as if each reference was individually incorporated by reference. Where a conflict exists between the instant application and a reference provided herein, the instant application shall dominate.

Claims

WHAT IS CLAIMED IS:
1. A compound of Formula I:
Figure imgf000086_0001
wherein:
R1 is hydrogen or Ci-6 alkyl;
R3a and R3b are each independently hydrogen, Ci-6 alkyl, Cvs cycloalkyl, or C4-14 alkyl-cycloalkyl;
R3c is hydrogen or Ci-6 alkyl;
alternatively, R2 and R3b are combined with the atoms to which they are attached to form a C5-8 heterocycloalkyl substituted with 1 to 3 R9 groups which are each independently hydrogen, Ci-6 alkyl, C s cycloalkyl, C3-14 alkyl-cycloalkyl, C4- 10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-18 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R9 groups attached to the same atom are combined to form =0;
alternatively, R2 and R3c are combined with the atoms to which they are attached to form a C5-8 cycloalkyl, substituted with 1 to 3 R10 groups which are each independently hydrogen, Ci-6 alkyl, C vs cycloalkyl, C3-14 alkyl-cycloalkyl, C4- 10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-I8 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R10 groups attached to the same atom are combined to form =0;
R4, R5, R6 and R7 are each independently hydrogen, Ci-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, halogen, Ci-6 haloalkyl, Ci-6 alkylamine, Ci-6 alkoxy, Ci-6 haloalkoxy, -OR8a, -NO2, -CN, -C(0)R8b, -C(0)0R8b, -0C(0)R8b, -0C(0)0R8b, -
Figure imgf000086_0002
S(0)2N(R8bR8c), Cvs cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-I8 alkyl-aryl, C5-10 heteroaryl, or C4- 16 alkyl-heteroaryl, wherein at least one of R4, R5, R6 and R7 is not H; alternatively, R4 and R5, R5 and R6, or R6 and R7 are combined with the atoms to which they are each attached to form a C3-6 cycloalkyl, C3-6 heterocycloalkyl, Ce-i2 aryl or C5-10 heteroaryl;
R8a, R8b, R8c and R8d are each independently H, Ci-6 alkyl;
wherein when R2 and R3b are combined with the atoms to which they are attached to form a C7 heterocycloalkyl, and R3a is methyl, then R5 is not OMe, OH, or Cl; wherein when R2 and R3b are combined with the atoms to which they are attached to form a C7 heterocycloalkyl, and R3a is ethyl, then R6 is not OMe; wherein when R2 and R3b are combined with the atoms to which they are attached to form a C7 heterocycloalkyl, and R6 is F, then at least one of R4, R5, and R7 is not hydrogen;
wherein when R2 and R3b are combined with the atoms to which they are attached to form a C7 heterocycloalkyl, and R6 is F, Cl, -Me, or -OMe, then R3a is -Me; wherein when R2 and R3b are combined with the atoms to which they are attached to form a C6 heterocycloalkyl, and R6 is Ci-6 alkyl, halogen, Ci-6 alkyoxy, - C(0)H, or -NH2, then at least one of R4, R5, and R7 is not hydrogen; and wherein when R2 and R3b are combined with the atoms to which they are attached to form a C6 heterocycloalkyl, and R6 is methyl, halogen, or -C(0)H, then at least one of R4, R5, and R7 is not methyl or Cl,
or pharmaceutically acceptable salts and isomers thereof.
2. The compound of claim 1, or a pharmaceutically acceptable salt thereof, wherein R1 is hydrogen or methyl.
3. The compound of claim 1 or 2, or a pharmaceutically acceptable salt thereof, wherein R1 is hydrogen.
4. The compound of any one of claims 1 to 3, or a pharmaceutically acceptable salt thereof, wherein R3a is hydrogen or Ci-6 alkyl.
5. The compound of any one of claims 1 to 4, or a pharmaceutically acceptable salt thereof, wherein R3a is hydrogen or methyl.
6. The compound of any one of claims 1 to 5, or a pharmaceutically acceptable salt thereof, wherein: R4, R5, R6 and R7 are each independently hydrogen, Ci-6 alkyl, halogen, Ci-6 haloalkyl, Ci-6 alkoxy, Ci-6 haloalkoxy, -OR8a, -NO2, -CN, -N(R8bR8c), - N(R8b)C(0)R8c, -C(0)N(R8bR8c), C3-8 cycloalkyl, C3-14 alkyl-cycloalkyl, Guo heterocycloalkyl, or C4-16 alkyl-heterocycloalkyl, wherein at least one of R4, R5, R6 and R7 is not H;
alternatively, R4 and R5, R5 and R6, or R6 and R7 are combined with the atoms to which they are each attached to form a C3-6 heterocycloalkyl; and R8a, R8b or R8c are each independently H, Ci-6 alkyl, or C3-6 cycloalkyl.
7. The compound of any one of claims 1 to 6, or a pharmaceutically acceptable salt thereof, wherein
R6 is Ci-6 alkyl, halogen, Ci-6 haloalkyl, Ci-6 alkoxy, Ci-6 haloalkoxy, -OR8a, -NO2, - CN, -N(R8bR8c), -N(R8b)C(0)R8c, -C(0)N(R8bR8c), C3-8 cycloalkyl, C3-14 alkyl- cycloalkyl, C4-10 heterocycloalkyl, or C4-16 alkyl-heterocycloalkyl; alternatively, R5 and R6 are combined with the atoms to which they are each attached to form a C3-6 heterocycloalkyl; and
R8a, R8b andR8c are each independently H, Ci-6 alkyl, or C3-6 cycloalkyl.
8. The compound of any one of claims 1 to 6, or a pharmaceutically acceptable salt thereof, wherein:
R4, R5, R6 and R7 are each independently hydrogen, Ci-6 alkyl, halogen, Ci-6
haloalkyl, Ci-6 alkoxy, Ci-6 haloalkoxy, -OR8a, -N(R8bR8c), -N(R8b)C(0)R8c, C4-10 heterocycloalkyl, or C4-16 alkyl-heterocycloalkyl, wherein at least one of R4, R5, R6 and R7 is not H;
alternatively, R5 and R6 are combined with the atoms to which they are each attached to form a C3-6 heterocycloalkyl; and
R8a, R8b and R8c are each independently H, Ci-6 alkyl, or C3-6 cycloalkyl.
9. The compound of any one of claims 1 to 8, or a pharmaceutically acceptable salt thereof, wherein:
R6 is Ci-6 alkyl, halogen, Ci-6 haloalkyl, Ci-6 alkoxy, Ci-6 haloalkoxy, -OR8a, - N(R8bR8c), -N(R8b)C(0)R8c, C4-10 heterocycloalkyl, or C4-16 alkyl- heterocycloalkyl;
alternatively, R5 and R6 are combined with the atoms to which they are each attached to form a C3-6 heterocycloalkyl; and R8a, R8b and R8c are each independently H, Ci-6 alkyl, or C3-6 cycloalkyl.
10. The compound of any one of claims 1 to 6 or 8, or a pharmaceutically acceptable salt thereof, wherein:
R4, R5, R6 and R7 are each independently hydrogen, Ci-6 alkyl, halogen, Ci-6
haloalkyl, Ci-6 alkoxy, Ci-6 haloalkoxy, -OR8a, -N(R8bR8c), or -N(R8b)C(0)R8c wherein at least one of R4, R5, R6 and R7 is not H;
R8a is H; and
R8b and R8c are each independently H or -Me;
alternatively, R5 and R6 are combined with the atoms to which they are each attached to form a C3-6 heterocycloalkyl.
1 1. The compound of any one of claims 1 to 6, 8 or 10, or a pharmaceutically acceptable salt thereof, wherein:
R4, R5, R6 and R7 are each independently H, F, Cl, Br, -OFI, -OMe, -CF3 -OCF3, -Me, -NMe2, -NHC(0)Me, or -N(Me)C(0)Me,
wherein at least one of R4, R5, R6 and R7 is not H;
alternatively, R5 and R6 are combined to form a 1,3-dioxole ring, or 1 ,4-dioxane ring.
12. The compound of any one of claims 1 to 6, 8, 10 or 11, or a pharmaceutically acceptable salt thereof, wherein:
R4 is H, F, -Me, -CF3, -OCF3, or -OMe;
R5 is H, F, Cl, Br, -Me, -CF3, -OCF3, -OH or -OMe;
R6 is H, F, -OH, -OMe, -OiPr, -Me, -CF3, -OCF3, -NMe2, -NHC(0)Me, or - N(Me)C(0)Me;
alternatively, R5 and R6 are combined to form a 1,3-dioxole ring or 1,4-dioxane ring; and
R7 is H, wherein at least one of R4, R5 and R6 is not H.
13. The compound of any one of claims 1 to 12, or a pharmaceutically acceptable salt thereof, wherein
R6 is F, -OH, -OMe, -OiPr, -Me, -CF3, -OCF3, -NMe2, -NHC(0)Me, or - N(Me)C(0)Me;
alternatively, R5 and R6 are combined to form a 1,3-dioxole ring or 1,4-dioxane ring.
14. The compound of any one of claims 1 to 13, or a pharmaceutically acceptable salt thereof, wherein R2 and R3b are combined with the atoms to which they are attached to form a C5-8 heterocycloalkyl substituted with 1 to 3 R9 groups which are each independently hydrogen, Ci-6 alkyl, Cvs cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10
heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-I8 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R9 groups attached to the same atom are combined to form =0.
15. The compound of any one of claims 1 to 14, or a pharmaceutically acceptable salt thereof, wherein R2 and R3b are combined with the atoms to which they are attached to form a C5-8 heterocycloalkyl.
16. The compound of any one of claims 1 to 15, or a pharmaceutically acceptable salt thereof, wherein R2 and R3b are combined with the atoms to which they are attached to form a C7-8 heterocycloalkyl.
17. The compound of any one of claims 1 to 16, or a pharmaceutically acceptable salt thereof, wherein the compound of Formula I has the following structure:
Figure imgf000090_0001
wherein:
R6 is Ci-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, halogen, Ci-6 haloalkyl, Ci-6 alkylamine, Ci-6 alkoxy, Ci-e haloalkoxy, -OR8a, -NO2, -CN, -C(0)R8b, -C(0)0R8b, -
Figure imgf000090_0002
C(0)C(0)N (R8bR8c), -S(02)R8b, -S(0)2N(R8bR8c), C3-8 cycloalkyl, C3-14 alkyl- cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7- 18 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl;
alternatively, R4 and R5, R5 and R6, or R6 and R7 are combined with the atoms to which they are each attached to form a C3-6 cycloalkyl, C3-6 heterocycloalkyl, Ce-n aryl or C5-10 heteroaryl;
R8a, R8b, R8c and R8d are each independently H, Ci-6 alkyl; and
n is 1, 2, or 3; wherein when n is 2 and R6 is F then at least one of R4, R5, and R7 is not hydrogen; wherein when n is 2 and R6 is F, Cl, methyl, or OMe, then R3a is methyl;
wherein when n is 1, and R6 is Ci-6 alkyl, halogen, Ci-6 alkyoxy, -C(0)FI, -NFI2, then at least one of R4, R5, and R7 is not hydrogen; and
wherein when n is 1 , and R6 is methyl, halogen, or -C(0)H, then at least one of R4, R5, and R7 is not methyl or Cl.
18. The compound of any one of claims 1 to 16, or a pharmaceutically acceptable salt thereof, wherein the compound of Formula I has the following structure:
Figure imgf000091_0001
19. The compound of any one of claims 1 to 18, or a pharmaceutically acceptable salt thereof, wherein R3a is methyl.
20. The compound of any one of claims 1 to 19, or a pharmaceutically acceptable salt thereof, wherein the compound of Formula I has the following structure:
Figure imgf000091_0002
21. The compound of any one of claims 1 to 20, or a pharmaceutically acceptable salt thereof, wherein the compound of Formula I has the following structure:
Figure imgf000091_0003
wherein:
R5 and R6 are each independently H, F, Cl, Br, I, -OFI, -OMe, -OiPr, -Me, -CF3,- OCF3, -NMe2, -NHC(0)Me, or -N(Me)C(0)Me,
wherein at least one of R5 and R6 is not H;
alternatively, R5 and R6 are combined to form a 1,3-dioxole ring or 1,4-dioxane ring.
22. The compound of any one of claims 1 to 21 , or a pharmaceutically acceptable salt thereof, wherein the compound of Formula I has the following structure:
Figure imgf000092_0001
wherein:
R5 and R6 are each independently F, Cl, Br, I, -OH, -OMe, -OiPr, -Me, -CF3,-OCF3, - NMe2, -NHC(0)Me, or -N(Me)C(0)Me;
alternatively, R5 and R6 are combined to form a 1,3-dioxole ring or 1,4-dioxane ring.
23. The compound of any one of claims 1 to 20, or a pharmaceutically acceptable salt thereof, wherein the compound of Formula I has the following structure:
Figure imgf000092_0002
wherein:
R4 and R6 are each independently H, F, Cl, Br, I, -OH, -OMe, -OiPr, -Me, -CF3,- OCF3, -NMe2, -NHC(0)Me, or -N(Me)C(0)Me,
wherein at least one of R4 and R6 is not H.
24. The compound of any one of claims 1 to 20 or 23, or a pharmaceutically acceptable salt thereof, wherein the compound of Formula I has the following structure:
Figure imgf000092_0003
wherein:
R4 and R6 are each independently F, Cl, Br, I, -OH, -OMe, -OiPr, -Me, -CF3,-OCF3, - NMe2, -NHC(0)Me, or -N(Me)C(0)Me.
25. The compound of any one of claims 1 to 20, or a pharmaceutically acceptable salt thereof, wherein the compound is
Figure imgf000093_0001
26. The compound of any one of claims 1 to 20 or 25, or a pharmaceutically acceptable salt thereof, wherein the compound is
Figure imgf000093_0002
27. The compound of any one of claims 1 to 20, or a pharmaceutically acceptable salt thereof, wherein the compound is
Figure imgf000093_0003
Figure imgf000094_0001
1 28. The compound of any one of claims 1 to 20, or a pharmaceutically
2 acceptable salt thereof, wherein the compound is
Figure imgf000095_0001
29. The compound of any one of claims 1 to 18, or a pharmaceutically acceptable salt thereof, wherein the compound of Formula I has the following structure:
Figure imgf000095_0002
30. The compound of any one of claims 1 to 18, or a pharmaceutically acceptable salt thereof, wherein the compound of Formula I has the following structure:
Figure imgf000095_0003
31. The compound of claim 30, or a pharmaceutically acceptable salt thereof, wherein the compound is
Figure imgf000095_0004
32. The compound of any one of claims 1 to 13, or a pharmaceutically acceptable salt thereof, wherein R2 and R3c are combined with the atoms to which they are attached to form a CVx cycloalkyl, substituted with 1 to 3 R10 groups which are each independently hydrogen, Ci-6 alkyl, C s cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-I8 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R10 groups attached to the same atom are combined to form =0.
33. The compound of any one of claims 1 to 12 or 32, or a pharmaceutically acceptable salt thereof, wherein R2 and R3c are combined with the atoms to which they are attached to form a C5-6 cycloalkyl, substituted with 1 to 2 R10 groups which are each hydrogen, or two R10 groups attached to the same atom are combined to form =0.
34. A compound, or a pharmaceutically acceptable salt thereof, wherein the compound is:
Figure imgf000096_0001
35. A pharmaceutical composition comprising a compound of any one of claims 1 to 34, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient.
36. A method for increasing neuronal plasticity, comprising contacting a neuronal cell with a compound of Formula I:
Figure imgf000096_0002
or a pharmaceutically acceptable salt thereof, in an amount sufficient to increase neuronal plasticity of the neuronal cell, wherein:
R1 is hydrogen or Ci-6 alkyl;
R3a and R3b are each independently hydrogen, Ci-6 alkyl, Cvs cycloalkyl, or C4-14 alkyl-cycloalkyl;
R3c is hydrogen or Ci-6 alkyl;
alternatively, R2 and R3b are combined with the atoms to which they are attached to form a C5-8 heterocycloalkyl substituted with 1 to 3 R9 groups which are each independently hydrogen, Ci-6 alkyl, C vs cycloalkyl, C3-14 alkyl-cycloalkyl, C4- 10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-18 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R9 groups attached to the same atom are combined to form =0;
alternatively, R2 and R3c are combined with the atoms to which they are attached to form a C5-8 cycloalkyl, substituted with 1 to 3 R10 groups which are each independently hydrogen, Ci-6 alkyl, C vs cycloalkyl, C3-14 alkyl-cycloalkyl, C4- 10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-I8 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R10 groups attached to the same atom are combined to form =0;
R4, R5, R6 and R7 are each independently hydrogen, Ci-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, halogen, Ci-6 haloalkyl, Ci-6 alkylamine, Ci-6 alkoxy, Ci-6 haloalkoxy,
Figure imgf000097_0001
S(0)2N(R8bR8c), Cvs cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-18 alkyl-aryl, C5-10 heteroaryl, or C4- 16 alkyl-heteroaryl, wherein at least one of R4, R5, R6 and R7 is not H;
alternatively, R4 and R5, R5 and R6, or R6 and R7 are combined with the atoms to which they are each attached to form a C3-6 cycloalkyl, C3-6 heterocycloalkyl, C6-12 aryl or C5-10 heteroaryl; and
R8a, R8b, R8c and R8d are each independently H, Ci-6 alkyl.
37. A method of treating a brain disorder, comprising administering to a subject in need thereof a therapeutically effective amount of a compound of Formula I:
Figure imgf000098_0001
or a pharmaceutically acceptable salt thereof, thereby treating the brain disorder, wherein:
R1 is hydrogen or Ci-6 alkyl;
R3a and R3b are each independently hydrogen, Ci-6 alkyl, Cvs cycloalkyl, or C4-14 alkyl-cycloalkyl;
R3c is hydrogen or Ci-6 alkyl;
alternatively, R2 and R3b are combined with the atoms to which they are attached to form a C5-8 heterocycloalkyl substituted with 1 to 3 R9 groups which are each independently hydrogen, Ci-6 alkyl, C s cycloalkyl, C3-14 alkyl-cycloalkyl, C4- 10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-18 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R9 groups attached to the same atom are combined to form =0;
alternatively, R2 and R3c are combined with the atoms to which they are attached to form a C5-8 cycloalkyl, substituted with 1 to 3 R10 groups which are each independently hydrogen, Ci-6 alkyl, C vs cycloalkyl, C3-14 alkyl-cycloalkyl, C4- 10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-18 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R10 groups attached to the same atom are combined to form =0;
R4, R5, R6 and R7 are each independently hydrogen, Ci-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, halogen, Ci-6 haloalkyl, Ci-6 alkylamine, Ci-6 alkoxy, Ci-6 haloalkoxy,
Figure imgf000098_0002
S(0)2N(R8bR8c), Cvs cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-18 alkyl-aryl, C5-10 heteroaryl, or C4- 16 alkyl-heteroaryl, wherein at least one of R4, R5, R6 and R7 is not H;
alternatively, R4 and R5, R5 and R6, or R6 and R7 are combined with the atoms to which they are each attached to form a C3-6 cycloalkyl, C3-6 heterocycloalkyl, C6-12 aryl or C5-10 heteroaryl; and
R8a, R8b, R8c and R8d are each independently H, Ci-6 alkyl.
38. The method of claim 37, wherein the brain disorder is a
neurodegenerative disorder, Alzheimer’s, or Parkinson’s disease.
39. The method of claim 37, wherein the brain disorder is a psychological disorder, depression, addiction, anxiety, or a post-traumatic stress disorder.
40. The method of claim 39, wherein the brain disorder is depression.
41. The method of claim 39, wherein the brain disorder is addiction.
42. The method of claim 37, wherein the brain disorder is treatment resistant depression, suicidal ideation, major depressive disorder, bipolar disorder, or substance use disorder.
43. The method of claim 37, wherein the brain disorder is schizophrenia.
44. The method of claim 37, wherein the brain disorder is alcohol use disorder.
45. The method of claim 37, wherein the brain disorder is stroke or traumatic brain injury.
46. The method of any one of claims 37 to 45, comprising administering one or more additional therapeutic agent that is lithium, Olanzapine (Zyprexa), Quetiapine (Seroquel), Risperidone (Risperdal), Ariprazole (Abilify), Ziprasidone (Geodon), Clozapine (Clozaril), divalproex sodium (Depakote), lamotrigine (Lamictal), valproic acid (Depakene), carbamazepine (Equetro), topiramate (Topamax), levomilnacipran (Fetzima), duloxetine (Cymbalta, Yentreve), venlafaxine (Effexor), citalopram (Celexa), fluvoxamine (Luvox), escitalopram (Lexapro), fluoxetine (Prozac), paroxetine (Paxil), sertraline (Zoloft), clomipramine (Anafranil), amitriptyline (Elavil), desipramine (Norpramin), imipramine (Tofranil), nortriptyline (Pamelor), phenelzine (Nardil), tranylcypromine (Parnate), diazepam (Valium), alprazolam (Xanax), or clonazepam (Klonopin).
47. A method for increasing at least one of translation, transcription or secretion of neurotrophic factors, comprising contacting a neuronal cell with a compound of Formula I:
Figure imgf000100_0001
or a pharmaceutically acceptable salt thereof, in an amount sufficient to increase neuronal plasticity of the neuronal cell, wherein:
R1 is hydrogen or Ci-6 alkyl;
R3a and R3b are each independently hydrogen, Ci-6 alkyl, Cvs cycloalkyl, or C4-14 alkyl-cycloalkyl;
R3c is hydrogen or Ci-6 alkyl;
alternatively, R2 and R3b are combined with the atoms to which they are attached to form a C5-8 heterocycloalkyl substituted with 1 to 3 R9 groups which are each independently hydrogen, Ci-6 alkyl, C s cycloalkyl, C3-14 alkyl-cycloalkyl, C4- 10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-I8 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R9 groups attached to the same atom are combined to form =0;
alternatively, R2 and R3c are combined with the atoms to which they are attached to form a C5-8 cycloalkyl, substituted with 1 to 3 R10 groups which are each independently hydrogen, Ci-6 alkyl, C vs cycloalkyl, C3-14 alkyl-cycloalkyl, C4- 10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-18 alkyl-aryl, C5-10 heteroaryl, or C4-16 alkyl-heteroaryl, or two R10 groups attached to the same atom are combined to form =0;
R4, R5, R6 and R7 are each independently hydrogen, Ci-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, halogen, Ci-6 haloalkyl, Ci-6 alkylamine, Ci-6 alkoxy, Ci-6 haloalkoxy,
Figure imgf000100_0002
S(0)2N(R8bR8c), Cvs cycloalkyl, C3-14 alkyl-cycloalkyl, C4-10 heterocycloalkyl, C4-16 alkyl-heterocycloalkyl, Ce-n aryl, C7-I8 alkyl-aryl, C5-10 heteroaryl, or C4- 16 alkyl-heteroaryl, wherein at least one of R4, R5, R6 and R7 is not H;
alternatively, R4 and R5, R5 and R6, or R6 and R7 are combined with the atoms to which they are each attached to form a C3-6 cycloalkyl, C3-6 heterocycloalkyl, C6-12 aryl or C5-10 heteroaryl; and 34 R8a, R8b, R8c and R8d are each independently H, Ci-6 alkyl.
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