WO2020057193A1

WO2020057193A1 - Fluorescence detection system for nucleic acid extraction and amplification

Info

Publication number: WO2020057193A1
Application number: PCT/CN2019/091421
Authority: WO
Inventors: 丁永军; 张中; 孙飞; 赵强; 刘振宇; 许德晨; 景宏维; 孙家振; 张国秀
Original assignee: 基蛋生物科技股份有限公司
Priority date: 2018-09-19
Filing date: 2019-06-14
Publication date: 2020-03-26
Also published as: CN108865659A

Abstract

Provided is a fluorescence detection system for nucleic acid extraction and amplification, falling within the field of biological sample analysis and detection. The fluorescence detection system for nucleic acid extraction and amplification comprises: a sample injection device, a kit transportation device, a sample pretreatment device, a nucleic acid extraction device, an amplification detection device and a mechanical arm system. The nucleic acid extraction device is set to extract and process a magnetic bead sample, and the amplification detection device is set to realize a high-throughput signal detection, with a relatively high degree of automation. By integrating the functions of sample injection, kit transportation, sample pretreatment, nucleic acid extraction, amplification detection and waste liquid treatment into one system, the degree of integration is high. Each of the functional modules of the fluorescence detection system for nucleic acid extraction and amplification is reasonable in layout and compact in structure. The whole fluorescence detection system for nucleic acid extraction and amplification has the characteristic of miniaturization, can be adapted to the requirements of individualized medicine, and is used in various medical institutions and for timely diagnosis, etc.

Description

Nucleic acid extraction and amplification fluorescence detection system

Technical field

The present application relates to the field of biological sample analysis and detection, and in particular, to a nucleic acid extraction and amplification fluorescence detection system.

Background technique

Biological sample analysis and testing requires a highly automated, highly integrated, and miniaturized diagnostic system.

"Automation" refers to the automation of the entire process of biomolecule detection and analysis of "sample preparation-target molecule enrichment or amplification-signal detection-data analysis".

"Integration" refers to the integration of functions such as sample storage, sample preparation, analytical reaction, signal detection and analysis that need to be automated equipment.

"Miniaturization" refers to the miniaturization of the automatic molecular diagnostic system to meet the requirements of individualized medical treatment, to be applied to the occasions of various medical institutions, and to timely diagnose.

Summary of the Invention

The purpose of this application is to provide a nucleic acid extraction and amplification fluorescence detection system with high degree of automation, high degree of integration, and miniaturization.

In order to achieve the foregoing objective, the technical solutions adopted in the embodiments of the present application are as follows:

A nucleic acid extraction and amplification fluorescence detection system includes: a sample injection device, the sample injection device includes a plurality of sample racks for placing sample bottles and a sample transport mechanism; a reagent kit transport device, and the reagent kit transport device includes a plurality of Kit storage assembly and kit transport mechanism; sample pretreatment device, sample pretreatment device is used to mix samples with reagents for heating, stirring and centrifugation; nucleic acid extraction device, nucleic acid extraction device is used to carry out sample extraction using reagents Nucleic acid extraction and magnetic separation; Amplification detection device, which is used to make nucleic acid processed samples into microchips for amplification and fluorescence detection analysis; and Robotic arm system, which is used to sample or reagent Transport to a sample injection device, a kit transport device, a sample pretreatment device, a nucleic acid extraction device, or an amplification detection device.

In a preferred embodiment of the present application, the reagent cartridge storage assembly includes a plurality of receiving chambers for storing the reagent cartridges and a cartridge case; each cartridge contains a cartridge case; a plurality of reagents The boxes are stacked in the magazine housing.

In a preferred embodiment of the present application, the kit transport mechanism includes a gripping component, a moving track, and a driving component; the gripping component is used to remove the reagent cartridge from the cartridge housing; the gripping component is drivingly connected to the driving component, To drive the gripping component to move along the moving track.

In a preferred embodiment of the present application, the sample pretreatment device includes a turntable and a suction component for placing the reagent box; the suction component is connected to the turntable and is located above the turntable, and the suction component can enter the reagent box to suck the test solution or Stir.

In a preferred embodiment of the present application, a heating component is provided at the bottom of the turntable for heating the test solution in the reagent box.

In a preferred embodiment of the present application, the amplification detection device includes a detection device, a microchip, and a chip transfer component; the microchip is used to accommodate a plurality of samples to be detected; the detection device includes a thermal cycle system and a real-time signal detection system The thermal cycling system is used to provide a suitable temperature for nucleic acid amplification of the samples in the microchip; the real-time signal detection system is used to perform fluorescence detection on the sample after the nucleic acid amplification; the chip transport component is used to grab the microchip and Transfer the microchip to the detection area of the detection device.

In a preferred embodiment of the present application, the nucleic acid extraction device includes a magnetic separator, a nucleic acid extraction reagent reservoir, a heater assembly, and a pipetting assembly; a magnetic separator is used to separate a test solution from a magnetic bead; a nucleic acid extraction reagent reservoir is used to Stores one or more reagents for extracting nucleic acid from a sample; a heater assembly is used to provide a suitable temperature for the nucleic acid extraction reaction.

In a preferred embodiment of the present application, the nucleic acid extraction and amplification fluorescence detection system further includes a waste liquid common liquid box; the waste liquid common liquid box is disposed between the nucleic acid extraction device and the sample sampling device.

In a preferred embodiment of the present application, each sample rack is provided with a plurality of receiving chambers for placing sample bottles; the sample transport mechanism includes a driving part and a moving guide; the driving part is drivingly connected to the moving guide; each The sample racks are connected to a moving guide.

In a preferred embodiment of the present application, the robotic arm system includes a suction arm and a gripping arm; the suction arm has used the suction test solution and moved it to the required position; the gripping arm is used to grip the test solution box and move it to the required position position.

The beneficial effects of this application are:

A nucleic acid extraction and amplification fluorescence detection system provided by the present application includes a sample sampling device, a kit transport device, a sample pretreatment device, a nucleic acid extraction device, an amplification detection device, and a robotic arm system. The sample injection device includes a plurality of sample racks for placing sample bottles and a sample transport mechanism. The kit transporting device includes a plurality of kit storage components and a kit transport mechanism. The sample pretreatment device is used for heating, stirring and centrifuging the sample and reagent after mixing. The nucleic acid extraction device is used for nucleic acid extraction and magnetic separation using an extraction reagent for a sample. The amplification detection device is used to make a micro-chip after the nucleic acid processed sample is used for amplification and fluorescence detection analysis. The robotic arm system is used to transport a sample or a reagent to a sample sampling device, a kit transport device, a sample pretreatment device, a nucleic acid extraction device, or an amplification detection device. The nucleic acid extraction and amplification fluorescence detection system extracts and processes magnetic bead samples by setting a nucleic acid extraction device, and realizes high-throughput signal detection by setting an amplification detection device, which has a high degree of automation. The nucleic acid extraction and amplification fluorescence detection system integrates various functions of sample injection, kit transportation, sample pretreatment, nucleic acid extraction, amplification detection, and waste liquid treatment into one system, and has a high degree of integration. The functional modules of the nucleic acid extraction and amplification fluorescence detection system have a reasonable layout and compact structure. The entire nucleic acid extraction and amplification fluorescence detection system has the characteristics of miniaturization, can meet the requirements of individualized medical treatment, and is applied to various medical institution occasions Prompt diagnosis, etc.

BRIEF DESCRIPTION OF THE DRAWINGS

In order to explain the technical solution of the embodiments of the present application more clearly, the drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of the present application, so It should be regarded as a limitation on the scope. For those of ordinary skill in the art, other related drawings can be obtained based on these drawings without paying creative work.

1 is a schematic structural diagram of a nucleic acid extraction and amplification fluorescence detection system according to a first embodiment of the present application;

FIG. 2 is an enlarged view at Ⅱ in FIG. 1;

FIG. 3 is a schematic structural diagram of a first perspective of a kit transport device of a nucleic acid extraction and amplification fluorescence detection system provided by a first embodiment of the present application; FIG.

FIG. 4 is a schematic structural view of a second perspective of a kit transport device of a nucleic acid extraction and amplification fluorescence detection system provided by the first embodiment of the present application; FIG.

5 is a schematic structural diagram of a cartridge case of a reagent cartridge transporting device of a nucleic acid extraction and amplification fluorescence detection system according to a first embodiment of the present application;

FIG. 6 is a schematic structural diagram of a first perspective of a sample pretreatment device of a kit transport device of a nucleic acid extraction and amplification fluorescence detection system provided by a first embodiment of the present application; FIG.

FIG. 7 is a schematic structural diagram of a second perspective of a sample pretreatment device of a kit transporting device of a nucleic acid extraction and amplification fluorescence detection system according to a first embodiment of the present application; FIG.

FIG. 8 is a schematic structural diagram of an amplification detection device of a kit transport device of a nucleic acid extraction and amplification fluorescence detection system provided by the first embodiment of the present application; FIG.

FIG. 9 is an enlarged view of a place in FIG. 1;

FIG. 10 is a schematic structural diagram of an amplification detection device of a kit transport device of a nucleic acid extraction and amplification fluorescence detection system provided by the first embodiment of the present application.

Icon: 10-nucleic acid extraction and amplification fluorescence detection system; 20-substrate; 21-first side; 22-second side; 23-third side; 24-fourth side; 100-sample sampling device; 110- Sample rack; 120-sample transport mechanism; 121-drive unit; 122-mobile guide; 200-reagent box transport device; 210-reagent box storage assembly; 211-receiving cavity; 212-clip case; 213-reagent box 220-kit transport mechanism; 221-clamping component; 222-moving track; 223-drive component; 300-sample pretreatment device; 310-turntable; 320-suction component; 330-heating component; 331-thermal conductive ring; 332-heating belt; 333-insulation layer; 400-amplification detection device; 410-detection device; 411-thermal cycle system; 412-real-time signal detection system; 420-micro chip; 430-chip transfer module; 500- Nucleic acid extraction device; 510-bracket; 520-fixer; 530-magnetic separator; 600-waste liquid common liquid box; 610-first layer containing section; 620-second layer containing section; 630-stirring device; 611-chamber; 700-robotic arm system; 710-suction arm; 711-first suction arm; 712-second suction arm; 713-third suction arm; 714-aspiration needle set; 715- Liquid needle body; 716-mounting plate; 720-grasping arm; 721-first grasping arm; 723-third grasping arm; 800-display system.

detailed description

In order to make the objectives, technical solutions, and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be clearly and completely described with reference to the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments These are part of the embodiments of the present application, but not all the embodiments. The components of embodiments of the present application, which are generally described and illustrated in the drawings herein, may be arranged and designed in a variety of different configurations.

Therefore, the following detailed description of the embodiments of the present application provided in the accompanying drawings is not intended to limit the scope of the claimed application, but merely represents selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by a person of ordinary skill in the art without creative efforts shall fall within the protection scope of the present application.

It should be noted that similar reference numerals and letters indicate similar items in the following drawings, so once an item is defined in one drawing, it need not be further defined and explained in subsequent drawings.

In the description of the embodiments of the present application, it should be noted that the orientations or positional relationships indicated by the terms "up" and "inner" are based on the orientations or positional relationships shown in the accompanying drawings, or the products used in this application are often used. The orientation or position relationship of the placement is only for the convenience of describing the application and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, structure and operation in a specific orientation, so it cannot be understood as limit.

In the description of this application, it should also be noted that the terms “setup”, “installation”, “connected”, and “connected” should be understood in a broad sense, for example, fixed connections, unless otherwise specified and limited. It can also be detachable or integrally connected; it can be directly connected, or it can be indirectly connected through an intermediate medium, and it can be the internal communication of two elements. For those of ordinary skill in the art, the specific meanings of the above terms in this application can be understood in specific situations.

Please refer to FIGS. 1 to 10. This embodiment provides a nucleic acid extraction and amplification fluorescence detection system 10, which includes a sample sampling device 100, a reagent cartridge transport device 200, a sample pretreatment device 300, an amplification detection device 400, The nucleic acid extraction device 500, the waste liquid common liquid box 600, and the robot arm system 700. The nucleic acid extraction and amplification fluorescence detection system 10 is provided with a nucleic acid extraction device 500 for extracting and processing magnetic bead samples, and the amplification detection device 400 is provided to realize high-throughput signal detection with a high degree of automation. The nucleic acid extraction and amplification fluorescence detection system 10 integrates various functions of sample injection, kit transportation, sample pretreatment, nucleic acid extraction, amplification detection, and waste liquid processing into one system, and has a high degree of integration. The functional modules of the nucleic acid extraction and amplification fluorescence detection system 10 have a reasonable layout and a compact structure. The entire nucleic acid extraction and amplification fluorescence detection system 10 has the characteristics of miniaturization, can meet the requirements of individualized medicine, and is applied to various medical institutions. Occasions and timely diagnosis.

Further, the nucleic acid extraction and amplification fluorescence detection system 10 includes a substrate 20, the aforementioned sample sampling device 100, kit transport device 200, sample pre-processing device 300, nucleic acid extraction device 500, amplification detection device 400, and waste liquid. The liquid tank 600 and the robot arm system 700 are both disposed on the substrate 20.

Further, in this embodiment, the shape of the substrate 20 is a square. The substrate 20 has a first side 21 and an opposite second side 22; and a third side 23 and an opposite fourth side 24. The sample pretreatment device 300 and the reagent cartridge transport device 200 are disposed on the first side 21 and the sample pretreatment device 300 is close to the third side 23. Therefore, it is convenient to transport the reagent kit to the sample pretreatment device 300. Further, the sample sampling device 100 is disposed on the third side 23 and is located at an end remote from the sample pre-processing device 300, so that the sample is conveniently transported away from the sample pre-processing device 300. Further, the nucleic acid extraction device 500 is disposed in the middle of the substrate 20, so that the sample injection device 100 can transport samples, the reagent container transport device 200 can transport reagents, and the sample pretreatment device 300 can transport processed samples. Further, the amplification detection device 400 is disposed on the second side 22 so that the sample on the nucleic acid extraction device 500 can be conveniently transferred to the amplification detection device 400.

Further, the reagent cartridge transporting apparatus 200 includes a plurality of reagent cartridge storage assemblies 210 and a reagent cartridge transport mechanism 220.

Further, the reagent cartridge storage assembly 210 includes a plurality of accommodating chambers 211 for storing reagent cartridges and a magazine holder 212; each of the accommodating chambers 211 houses a magazine holder 212; a plurality of reagent cartridges 213 Stacked inside the magazine housing 212. Further, the reagent cartridge transport mechanism 220 includes a gripping component 221, a moving track 222, and a driving component 223. Further, the gripping component 221 is used to grip the reagent cartridge 213 out of the cartridge housing 212; the gripping component 221 is drivingly connected to the driving component 223 to drive the gripping component 221 to move along the moving track 222.

Further, the sample pre-processing device 300 is used for heating, stirring and centrifuging the sample after mixing the sample with the reagent. Specifically, the sample pretreatment device 300 includes a turntable 310 and a suction component 320 for placing a reagent kit; the suction component 320 is connected to the turntable 310 and is located above the turntable 310, and the suction component 320 can enter the reagent cartridge to suck a test solution or Stir. Further, a heating component 330 is provided at the bottom of the turntable 310 for heating the test solution in the reagent box. The turntable 310 is provided with a plurality of accommodating portions for accommodating a reagent cartridge. During the rotation of the turntable 310, the suction component 320 is used for stirring.

Further, the heating assembly 330 includes a thermally conductive ring 331, a heating belt 332, and a heat insulation layer 333. A heat conducting ring 331 is provided at the bottom of each accommodating portion; a heating belt 332 is provided below the heat conducting ring 331. A heat insulation layer 333 is further provided between the heating belt 332 and the bottom wall of the turntable 310. Therefore, the diffusion of heat is avoided, and the structural reliability of the entire sample pre-processing device 300 is further improved.

Further, the amplification detection device 400 is configured to perform nucleic acid extraction and magnetic separation on the pretreated sample by using an extraction reagent. Specifically, the amplification detection device 400 includes a detection device 410, a microchip 420, and a chip transfer component 430. The microchip 420 is used for accommodating a plurality of samples to be detected. The detection device 410 includes a thermal cycling system 411 and a real-time signal detection system 412. The thermal cycling system 411 is used to provide a suitable temperature for nucleic acid amplification of a sample in the microchip 420. The real-time signal detection system 412 is used to perform fluorescence detection on a sample after nucleic acid amplification. The chip transfer component 430 is used for grasping the microchip 420 and transferring the microchip 420 to a detection area of the detection device 410.

Further, the nucleic acid extraction device 500 includes a magnetic separator 530, a nucleic acid extraction reagent reservoir, a heater assembly, and a pipetting assembly. The magnetic separator 530 is used to separate the test solution from the magnetic beads; the nucleic acid extraction reagent reservoir is used to store one or more reagents for extracting nucleic acid from the sample; the heater assembly is used to provide a suitable temperature for the nucleic acid extraction reaction. Specifically, the above-mentioned magnetic separator 530, nucleic acid extraction reagent reservoir, and heater assembly are all disposed on a plurality of brackets 510, each configured to receive a certain number of samples and a corresponding number of holders 520. Each of these holders 520 includes a processing chamber, a waste chamber, one or more pipette tips, and one or more receptacles. One or more of the reservoirs each contain a sufficient amount of one or more reagents for performing nucleic acid extraction from the sample. A magnetic separator 530 configured to move relative to the processing chamber of each holder 520. A heater assembly configured to independently heat each processing chamber. And a liquid dispenser or the like configured to perform a fluid transfer operation on two or more holders 520 simultaneously.

Further, the waste liquid common liquid cartridge 600 is disposed between the nucleic acid extraction device 500 and the sample sampling device 100. Specifically, the waste liquid common liquid box 600 includes a first-layer accommodating portion 610, a second-layer accommodating portion 620, and a stirring device 630. The first-layer accommodating portion 610 includes a plurality of chambers 611 which are not connected to each other. Each chamber 611 has at least one inlet. At least one chamber 611 is a waste liquid collection chamber. The second layer accommodating portion 620 is disposed below the first layer accommodating portion 610. The second layer accommodating portion 620 is configured to contain waste liquid. Each waste liquid collection chamber is connected to the second-layer accommodating portion 620. The stirring device 630 is connected to the waste liquid collection chamber. The space layout of the multiple chambers 611 and the stirring device 630 of the waste liquid common liquid box 600 is compact and reasonable, which is convenient for operators. Providing a common reagent storage section and a waste liquid collection section in one box not only saves space, but also reduces the number of times the user needs to replace consumables, saves time, and improves the efficiency of use. The setting of the stirring device 630 solves the problem of precipitation of magnetic beads, and the use effect is better.

Further, the sample injection device 100 includes a plurality of sample racks 110 for placing sample bottles and a sample transport mechanism 120. Further, each sample rack 110 is provided with a plurality of receiving chambers for placing sample bottles; the sample transport mechanism 120 includes a driving part 121 and a moving guide 122; the driving part 121 is drivingly connected to the moving guide 122; each sample The racks 110 are all connected to the moving rail 122. Specifically, in this embodiment, the sample injection device 100 includes 4 rows of sample racks 110 for sampling, and each row of the sample racks 110 can store 12 sample bottles.

Further, the robot arm system 700 is configured to transport a sample or a reagent to the sample injection device 100, the reagent cartridge transport device 200, the sample pretreatment device 300, the nucleic acid extraction device 500, or the amplification detection device 400. Further, the robot arm system 700 includes a suction arm 710 and a grasping arm 720. Further, the sucking arm 710 is used to suck the test solution and move to the required position; the gripping arm 720 is used to grab the test solution box and move to the required position. Specifically, in this embodiment, the suction arm 710 includes a first suction arm 711 provided along the extending direction of the first side 21 of the substrate 20, a second suction arm 712 provided along the extending direction of the second side 22 of the substrate 20, and a sliding arrangement The third suction arm 713 and the liquid suction needle group 714 between the first suction arm 711 and the second suction arm 712. The liquid suction needle set 714 is slidably connected to the third suction arm 713, and can move back and forth on the third suction arm 713. At the same time, when the third suction arm 713 slides on the second suction arm 712 and the first suction arm 711, it can be realized The aspiration needle group 714 moves in both X and Y directions. Further, the aspiration needle group 714 includes an aspiration needle body 715 and a mounting plate 716, and the aspiration needle body 715 is slidably connected to the installation plate 716, thereby realizing the movement of the aspiration needle body 715 in the Z-axis direction, thereby making the entire aspiration The arm 710 can move over the entire range of the substrate 20.

Further, the gripping arm 720 is disposed inside the suction arm 710 and has a lower height than the gripping arm 720. The grasping arm 720 is mainly used for grasping a reagent cartridge. The grasping arm 720 includes a first grasping arm 721, a second grasping arm (not shown), a third grasping arm 723, and a jaw assembly (not shown). The first grasping arm 721 is connected to the back plate provided on the fourth side 24 of the substrate 20 and is disposed along the extending direction of the fourth side 24; the second grasping arm is disposed along the direction of the first side 21 and is vertically connected to the first A grasping arm 721 is connected to the first grasping arm 721 in a direction perpendicular to the plane of the substrate 20. The gripper assembly is slidably connected to the second grasping arm, so that when the gripper assembly can move in three directions of XYZ, the reagent cartridge is gripped.

It should be understood that, for the specific structure of the above-mentioned gripper assembly, a gripper structure commonly used in the art and capable of gripping a reagent cartridge can be selected.

Further, the nucleic acid extraction and amplification fluorescence detection system 10 is further provided with a display system 800 so that detection data can be displayed.

The above description is only a preferred embodiment of the present application, and is not intended to limit the present application. For those skilled in the art, this application may have various modifications and changes. Any modification, equivalent replacement, or improvement made within the spirit and principle of this application shall be included in the protection scope of this application. It should be noted that similar reference numerals and letters indicate similar items in the following drawings, so once an item is defined in one drawing, it need not be further defined and explained in subsequent drawings.

Claims

A nucleic acid extraction and amplification fluorescence detection system, comprising:

A sample injection device, which includes a plurality of sample racks for placing sample bottles and a sample transport mechanism;

A reagent kit transporting device, which includes a plurality of reagent kit storage components and a reagent kit transporting mechanism;

A sample pretreatment device, which is used for heating, stirring and centrifuging the sample after mixing the sample with the reagent;

A nucleic acid extraction device for nucleic acid extraction and magnetic separation using an extraction reagent for a sample;

An amplification detection device for performing amplification and fluorescence detection analysis after the nucleic acid-treated sample is made into a microchip; and

A robotic arm system for transporting the sample or reagent to the sample injection device, the kit transport device, the sample pretreatment device, the nucleic acid extraction device, or the amplification Detection device.
The nucleic acid extraction and amplification fluorescence detection system according to claim 1, wherein:

The kit storage assembly includes a plurality of accommodating chambers for storing reagent kits, and a cartridge case; each of the accommodating chambers contains one cartridge case; and a plurality of the reagent cartridge stacks. Placed in the magazine case.
The nucleic acid extraction and amplification fluorescence detection system according to claim 2, wherein:

The kit transport mechanism includes a gripping component, a moving track, and a driving component;

The gripping component is used for gripping and removing the reagent cartridge from the magazine housing; the gripping component is drivingly connected to the driving component to drive the gripping component to move along the moving track.
The nucleic acid extraction and amplification fluorescence detection system according to claim 1, wherein:

The sample pretreatment device includes a turntable and a suction component for placing a reagent kit; the suction component is connected to the turntable and is located above the turntable, and the suction component is capable of entering the reagent cartridge for a suction test Liquid or stir.
The nucleic acid extraction and amplification fluorescence detection system according to claim 4, wherein:

The bottom of the turntable is provided with a heating component for heating the test solution in the reagent box.
The nucleic acid extraction and amplification fluorescence detection system according to claim 1, wherein:

The amplification detection device includes a detection device, a microchip, and a chip transporting component; the microchip is used to accommodate a plurality of samples to be detected; the detection device includes a thermal cycle system and a real-time signal detection system, and the thermal The circulation system is used to provide a suitable temperature for nucleic acid amplification of the sample in the microchip; the real-time signal detection system is used to perform fluorescence detection on the sample after the nucleic acid amplification occurs; the chip is transported The component is used for grasping the microchip and transferring the microchip to a detection area of the detection device.
The nucleic acid extraction and amplification fluorescence detection system according to claim 1, wherein:

The nucleic acid extraction device includes a magnetic separator, a nucleic acid extraction reagent reservoir, a heater assembly, and a pipetting assembly;

The magnetic separator is used to separate a test solution from magnetic beads; the nucleic acid extraction reagent reservoir is used to store one or more reagents for extracting nucleic acid from a sample; and the heater assembly is used to provide a suitable temperature for a nucleic acid extraction reaction.
The nucleic acid extraction and amplification fluorescence detection system according to any one of claims 1 to 7, wherein:

The nucleic acid extraction and amplification fluorescence detection system further includes a waste liquid common liquid box; the waste liquid common liquid box is disposed between the nucleic acid extraction device and the sample sampling device.
The nucleic acid extraction and amplification fluorescence detection system according to claim 8, wherein:

Each of the sample racks is provided with a plurality of receiving chambers for placing sample bottles;

The sample transport mechanism includes a driving section and a moving guide; the driving section is drivingly connected to the moving guide; each of the sample racks is connected to the moving guide.
The nucleic acid extraction and amplification fluorescence detection system according to claim 1, wherein:

The robotic arm system includes a suction arm and a grasping arm;

The suction arm is used to suck the test solution and move it to the required position; the grasping arm is used to grab the test solution box and move it to the required position.