WO2016206599A1 - Array bioimpedance measurement probe for tissue and measurement method thereof - Google Patents
Array bioimpedance measurement probe for tissue and measurement method thereof Download PDFInfo
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- WO2016206599A1 WO2016206599A1 PCT/CN2016/086864 CN2016086864W WO2016206599A1 WO 2016206599 A1 WO2016206599 A1 WO 2016206599A1 CN 2016086864 W CN2016086864 W CN 2016086864W WO 2016206599 A1 WO2016206599 A1 WO 2016206599A1
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- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/05—Detecting, measuring or recording for diagnosis by means of electric currents or magnetic fields; Measuring using microwaves or radio waves
- A61B5/053—Measuring electrical impedance or conductance of a portion of the body
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- the invention belongs to the field of bioimpedance measurement, and in particular relates to an array biological tissue impedance measuring probe and a measuring method thereof.
- the coverage of the impedance detection electrode is required to be small enough, but when the impedance is detected After the electrode coverage is small enough, it is necessary to repeatedly perform multiple detections on the measured tissue, and it is easy to cause missed detection, resulting in low detection efficiency, thereby reducing measurement effectiveness.
- an object of the present invention is to provide an array type biological tissue impedance measuring probe and a measuring method thereof which have high measurement efficiency, low miss detection rate, and improved measurement effectiveness.
- An array type biological tissue impedance measuring probe comprises a substrate and an electrode array embedded on the substrate, the electrode array forming a plurality of detection regions for performing bioimpedance measurement in units of four microelectrodes, the plurality of detections
- the area includes a minimum detection area formed by biological impedance measurement covering the biological tissue to be tested with four microelectrodes near the center, and each detection area overlaps with the smallest detection area as a center.
- the electrode array is formed by arranging a plurality of micro electrodes symmetrically distributed in a center.
- the four micro electrodes of each detection area are respectively located at four corners of the square.
- the electrode array is formed by arranging a plurality of electrodes extending at both ends of a diagonal line of four microelectrodes of a minimum detection area.
- the four microelectrodes of each detection region have any two adjacent microelectrodes as excitation electrode pairs, and any two adjacent microelectrodes are collection electrode pairs.
- the method further includes an excitation unit, a switching unit, and an acquisition signal unit, wherein the probe is respectively connected to the excitation unit and the acquisition signal unit through a switching unit.
- the switching unit is a multi-select switch or a switch array composed of a plurality of multiple-select switches.
- An array type biological tissue impedance measuring probe comprises a substrate and an electrode array embedded on the substrate, wherein the electrode array is uniformly distributed by a plurality of electrodes, and is uniformly distributed in units of four electrodes for use in living organisms a plurality of detection regions of the impedance measurement; the plurality of detection regions include a minimum detection region formed by covering the biological tissue to be tested during bioimpedance measurement with four electrodes near the center, and each detection region has a minimum detection region The centers are overlapped with each other.
- the electrode array is formed by arranging a plurality of electrodes symmetrically distributed in a center.
- the four electrodes of each detection area are respectively located at four corners of the square.
- the electrode array is formed by arranging a plurality of electrodes extending at both ends of a diagonal of four electrodes of a minimum detection area.
- the four electrodes of each detection area have any adjacent two electrodes as excitation electrode pairs, and any adjacent two electrodes are collection electrode pairs.
- the method further includes an excitation unit, a switching unit, and an acquisition signal unit, wherein the probe is respectively connected to the excitation unit and the acquisition signal unit through a switching unit.
- the switching unit is a multi-select switch or a switch array composed of a plurality of multiple-select switches.
- Characteristic parameter set X(i), where i 1, 2, 3...n;
- the invention adopts the above-mentioned electrode array arrangement and switching control electrode signal excitation and signal acquisition, and realizes the omnidirectional no-dead detection of different depths and areas of the biological tissue to be tested by changing the actual detection coverage of the biological tissue to be tested, thereby effectively Avoid missed detection or misdetection; at the same time, it is also helpful to assist the biological tissue to be measured to locate the position of a certain tissue in the impedance characteristic parameter; and to switch the detection area detection of different scales of the biological tissue to be tested, and compare the measured impedance spectral characteristic parameters Whether it conforms to the relevant organizational characteristics of the biological tissue impedance spectrum database, compared with the prior art, the detection efficiency is improved, the missed detection rate is lowered, and a certain organization is more accurately positioned.
- the invention is particularly suitable for the detection of small biological tissue samples to be tested. It is especially suitable for array microscopic and macroscopic analysis of smaller biological tissues to be tested.
- FIG. 1 is a schematic view showing an electrode array distribution of a measuring probe according to Embodiment 1 of the present invention
- FIG. 2 is a schematic structural view of a measuring probe according to Embodiment 1 of the present invention.
- FIG. 3 is a schematic view showing the operation of four microelectrodes in the detection area according to the first embodiment of the present invention
- FIG. 4 is a schematic diagram showing an electrode array distribution of a measuring probe according to Embodiment 2 of the present invention.
- FIG. 5 is a schematic diagram of a basic flow of a measurement method according to Embodiment 3 of the present invention.
- Figure 6 is a schematic view of the distribution of tissue P1 in the biological tissue to be tested according to the present invention.
- Fig. 7 is a schematic view showing the distribution of tissue P1 in the biological tissue to be tested according to the present invention.
- a four-electrode impedance or two-electrode impedance measurement scheme with a fixed electrode tip size is commonly used.
- the prior art solution has a fixed electrode size and arrangement, and is sensitive only to detection targets within a specific size and depth, and has limited resolution; and it is also required to select points on the biological tissue to be tested and measure multiple times, resulting in The measurement of the biological tissue to be tested is low, and it is easy to miss or misdetect.
- the present invention provides an array type biological tissue impedance measuring probe and a measuring method thereof. The following description is only a preferred embodiment of the invention and is not intended to limit the scope of the invention.
- an embodiment of the present invention provides an array type biological tissue impedance measuring probe 10 including a substrate 11 and an electrode array 12 embedded on the substrate 11.
- the electrode array is formed by 12 with four micro electrodes E. 1 , E 2 , E 3 , E 4 (E 5 , E 6 , E 7 , E 8 or E 9 , E 10 , E 11 , E 12 ) are a plurality of detection regions E 1 E 2 for bioimpedance measurement of the unit E 3 E 4 , E 5 E 6 E 7 E 8 , E 9 E 10 E 11 E 12 , the plurality of detection regions include four microelectrodes E 1 , E 2 , E 3 , E 4 near the center Performing a minimum detection area formed by bioimpedance measurement covering the biological tissue to be tested, and each detection area E 1 E 2 E 3 E 4 , E 5 E 6 E 7 E 8 , E 9 E 10 E 11 E 12 with minimum detection
- the regions E 1 E 2 E 3 E 4 are centered on each other.
- the four microelectrodes E 1 , E 2 of each of the detection regions E 1 E 2 E 3 E 4 , E 5 E 6 E 7 E 8 , E 9 E 10 E 11 E 12 , E 3 , E 4 (E 5 , E 6 , E 7 , E 8 or E 9 , E 10 , E 11 , E 12 ) are respectively located at the four corners of the square.
- the spacing between adjacent electrodes is less than P or greater than or equal to 2P, where P is 2 mm.
- the measurement probe further includes an excitation unit 13, a switching unit 14, and an acquisition signal unit 15, wherein the probe 10 and the excitation unit 13 and the acquisition signal are respectively passed through the switching unit 14.
- Unit 15 is connected.
- the four microelectrodes of each detection region have any two adjacent microelectrodes as excitation electrode pairs, and any adjacent two microelectrodes are collection electrode pairs, wherein
- the excitation electrode pair applies an excitation positive and negative signal, and the collection electrode pair is responsible for collecting a voltage signal or a current signal on the excited target (the biological tissue to be tested); switching control by the switching unit 14 causes the collection electrode pair to cover each group of adjacent electrodes and
- the bioimpedance measurement is performed by signal acquisition to achieve a full range of bioimpedance measurement of the biological tissue to be measured.
- the switching unit 14 in the embodiment of the present invention is a switch array composed of multiple switches or multiple switches.
- an embodiment of the present invention provides an array type biological tissue impedance measuring probe 10, comprising a substrate 11 and an electrode array 12 embedded on the substrate 11, the electrode array forming 12 with four micro electrodes E 1 ', E 2 ', E 3 ', E 4 ' (E 5 ', E 6 ', E 7 ', E 8 ' or E 9 ', E 10 ', E 11 ', E 12 ')
- the detection area includes a minimum detection area E 1 'E 2 ' formed by biological impedance measurement covering the biological tissue to be tested with four microelectrodes E 1 ', E 2 ', E 3 ', and E 4 ' near the center.
- the minimum detection areas E 1 'E 2 'E 3 'E 4 ' are centered on each other.
- the plurality of micro-electrodes of the electrode array 12 is a centrosymmetric distribution E 1, E 2, ...... E n arrayed.
- each of the detection areas E 1 'E 2 'E 3 'E 4 ', E 5 'E 6 'E 7 'E 8 ', E 9 'E 10 'E 11 'E 12 'four microelectrodes E 1 ', E 2 ', E 3 ', E 4 ' (E 5 ', E 6 ', E 7 ', E 8 ' or E 9 ', E 10 ', E 11 ', E 12 ') are located at the four corners of the square.
- the electrode array 12 is composed of a plurality of electrodes E 1 ', E 2 ', and ... extending across the diagonal line E 1 'E 3 ' or E 2 'E 4 ' of the four microelectrodes of the smallest detection area.
- E n ' is arranged.
- the measurement probe according to the embodiment of the present invention further includes an excitation unit 13, a switching unit 14, and an acquisition signal unit 15, wherein the probe 10 and the excitation unit are respectively connected by the switching unit 14.
- the element 13 is connected to the acquisition signal unit 15.
- the switching unit 14 in the embodiment of the present invention is a switch array composed of multiple switches or multiple switches.
- the excitation and collection modes of the four micro-electrodes of each detection area are the same as those shown in FIG. 3, and details are not described herein again.
- the electrode array arrangement and the switching control electrode signal excitation and signal acquisition are adopted, and the actual detection coverage of the biological tissue to be tested is changed to realize different depths of the biological tissue to be tested and
- the area has no dead angle detection, which effectively avoids missed detection or misdetection; it also helps to locate the position of an organization in the measured impedance characteristic parameters of the biological tissue to be tested. It is especially suitable for array microscopic and macroscopic analysis of smaller biological tissues to be tested.
- an embodiment of the present invention provides an array type bio-impedance measurement method using the measurement probe described in the above technical solution, the measurement
- the contact surface of the probe and the biological tissue to be tested forms a plurality of detection regions detected by the four micro electrodes through the switching unit switching control electrode array, wherein the specific steps of the measurement method are as follows:
- Characteristic parameter set X(i), where i 1, 2, 3...n, wherein the minimum detection area E 1 E 2 E 3 E 4 is four electrodes E 1 , E 2 , E 3 near the center, The electrode group formed by E 4 detects the covered area;
- the measurement method in the embodiment of the present invention is specifically to first contact the measurement probe 10 according to the first embodiment of the present invention with the biological tissue to be tested; wherein the detection area of the tissue to be tested is E 1 E 2 E 3 E 4 , E 5 E 6 E 7 E 8 and E 9 E 10 E 11 E 12 and the area relationship of each detection area is The first impedance spectral characteristic parameters X(1), X(2), and X(3) of each detection region that are controlled by the switching unit are sequentially switched from large to small according to the area of the detection area, to the minimum detection area E 1 E 2 E 3 E 4 stops switching, where X(1) is the first impedance spectral characteristic parameter of detection area E 1 E 2 E 3 E 4 and X(2) is the first impedance of detection area E 5 E 6 E 7 E 8 The spectral characteristic parameter, X(3) is the first impedance spectral characteristic parameter of the detection region E 9 E 10 E 11 E 12 , so the obtained first impedance spectral characteristic parameter set is detected
- the detected first impedance spectral characteristic parameters X(1), X(2), and X(3) are respectively compared with a clinical biological tissue impedance database. According to the comparison result, it is judged whether there is an intersection of the detection area corresponding to a certain tissue impedance characteristic and another detection area to verify the accuracy of the impedance spectrum characteristic parameter of the biological tissue to be tested.
- determining X(1) and X(2) of the corresponding detection regions E 1 E 2 E 3 E 4 and E 5 E 6 E 7 E 8 in the biological tissue to be tested according to the comparison analysis result Meets the organization's P1 impedance characteristics. According to the result of the comparison analysis, it is determined that the detection areas covering the tissue P1 in the biological tissue to be tested are E 1 E 2 E 3 E 4 and E 5 E 6 E 7 E 8 , and the detection areas having the coincident intersection in the detection area are statistically detected. Two, so verify that the measured characteristic parameters of the measured biological tissue impedance are accurate.
- the position of the tissue in the biological tissue to be tested is located according to a certain tissue impedance characteristic, for example, the test area covered by the detection area E 9 E 10 E 11 E 12
- the area covered by the biological tissue area relative to the tissue P1 is small, and the impedance characteristics conforming to the tissue P1 may not be detected, and the detection areas E 1 E 2 E 3 E 4 and E 5 E 6 E 7 E 8 conform to the impedance of the tissue P1.
- the tissue P1 is located in the detection area E 1 E 2 E 3 E 4 or E 5 E 6 E 7 E 8 .
- the measurement method in the embodiment of the present invention is specifically to first contact the measurement probe 10 described in the second embodiment of the present invention with the biological tissue to be tested; wherein the detection area of the tissue to be tested is E 1 'E 2 'E 3 'E 4 ', E 5 'E 6 ', E 7 'E 8 ' and E 9 'E 10 'E 11 'E 12 ' and the area relationship of each detection area is S E1 'E2'E3'E4' ⁇ S E5'E6'E7'E8' ⁇ S E9'E10'E11'E12' , the first impedance spectral characteristic parameter X of each detection area controlled by the switching unit according to the area of the detection area in turn from large to small (1), X(2) and X(3), stopping switching after the minimum detection area E 1 'E 2 'E 3 'E 4 ', where X(1) is the detection area E 1 'E 2 'E 3
- the detection regions E 1 'E 2 'E 3 'E 4 ' and E corresponding to X(1), X(2), and X(3) in the biological tissue to be tested are determined based on the results of the comparison analysis.
- 5 'E 6 'E 7 'E 8 ′′ has a tissue P1 impedance characteristic.
- the detection area corresponding to the tissue P1 impedance characteristic covering the biological tissue to be tested is E 1 'E 2 'E 3 'E 4 ' and E 5 'E 6 'E 7 'E 8 ', there are two detection areas with coincident intersections in the detection area by statistics, so it is verified that the measured impedance characteristic parameters of the biological tissue to be tested are accurate.
- the position of the tissue in the biological tissue to be tested is located according to a certain tissue impedance characteristic, for example, due to the detection area E 9 'E 10 'E 11 'E 12 '
- the area of the covered biological tissue to be covered is small relative to the area covered by the tissue P1, and the impedance characteristics conforming to the tissue P1 may not be detected, and the detection areas E 1 'E 2 'E 3 'E 4 ' and E 5 'E 6 'E 7 'E 8 ' conforms to the impedance characteristic of tissue P1, and the tissue P1 is located in the detection area E 1 'E 2 'E 3 'E 4 ' or E 5 'E 6 'E 7 'E 8 '.
- the array type bioimpedance tissue measuring method can efficiently obtain the detection areas of the whole biological tissue to be tested according to the detection array of multiple sets of micro bio-impedance detecting electrodes and the statistical analysis processing method. Microscopic impedance spectrum characteristics, and through the comparative analysis with the clinical biological tissue impedance database, and statistical verification, confirm whether abnormal tissue in the biological tissue. At the same time, the invention can accurately locate the position of the abnormal tissue by analyzing the impedance spectrum characteristics between the detection regions.
- the fourth embodiment of the present invention can distribute the same type of electrodes on the same array electrode contact surface, thereby saving the space occupied by the electrode arrangement.
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Abstract
An array bioimpedance measurement probe (10) for a tissue and measurement method thereof. The probe (10) comprises a substrate (11) and an electrode array (12) embedded in the substrate (11). The electrode array (12) forms multiple test regions used to perform bioimpedance measurement with four microelectrodes (121) as a unit. The multiple test regions each comprise a minimal test region formed by the four microelectrodes (121) close to the center and used to perform bioimpedance measurement on a covered biological tissue (20) to be tested. The test regions overlap with each other with the minimal test regions as the centers. Therefore, the present invention provides a comprehensive test on a biological tissue (20) to be tested including different depths and areas, thus preventing omissions and errors in the test. In addition, the present invention facilitates positioning of some tissue (P1) corresponding to a tested impedance characteristic parameter in the biological tissue (20) to be tested.
Description
本发明属于生物阻抗测量领域,尤其是涉及一种阵列式生物组织阻抗测量探头及其测量方法。The invention belongs to the field of bioimpedance measurement, and in particular relates to an array biological tissue impedance measuring probe and a measuring method thereof.
目前,在医院临床组织活检过程中,通常采用冰冻活检或者石蜡活检进行,二者的检测结果对检验人员的个体识别能力有很大要求,因此具备很大的主观性。科学研究表明:不同的组织具备不同的阻抗频谱特征,因此通过测量目标的生物阻抗频谱特性能够准确识别组织类型,但是在实际操作过程中,需要检测的组织形态各异,尤其是异常病变部分大小各异,且可能存在于被测组织的任何部位,导致测量结果无法有效全面反映组织特征,如果要准确地测量到病变组织的阻抗特征,就需要阻抗检测电极的覆盖面足够小,然而当阻抗检测电极覆盖面足够小之后就需要对被测组织重复进行多次检测,并且容易造成漏检,导致检测效率低,从而降低了测量有效性。At present, in the clinical biopsy process of hospitals, frozen biopsy or paraffin biopsy is usually used. The test results of the two have great requirements on the individual identification ability of the inspectors, so they are very subjective. Scientific research shows that different tissues have different impedance spectrum characteristics, so the bio-impedance spectrum characteristics of the target can be accurately identified, but in the actual operation process, the tissue to be detected is different, especially the size of the abnormal lesion. Different, and may exist in any part of the measured tissue, resulting in the measurement results can not effectively reflect the characteristics of the tissue. If the impedance characteristics of the diseased tissue are to be accurately measured, the coverage of the impedance detection electrode is required to be small enough, but when the impedance is detected After the electrode coverage is small enough, it is necessary to repeatedly perform multiple detections on the measured tissue, and it is easy to cause missed detection, resulting in low detection efficiency, thereby reducing measurement effectiveness.
发明内容Summary of the invention
针对上述现有技术存在的不足,本发明的目的是提供一种测量效率高、漏检率低以及提高测量有效性的阵列式生物组织阻抗测量探头及其测量方法。In view of the deficiencies of the prior art described above, an object of the present invention is to provide an array type biological tissue impedance measuring probe and a measuring method thereof which have high measurement efficiency, low miss detection rate, and improved measurement effectiveness.
为了实现上述目的,本发明所采用的技术方案如下:In order to achieve the above object, the technical solution adopted by the present invention is as follows:
一种阵列式生物组织阻抗测量探头,包括基板和嵌于所述基板上的电极阵列,所述电极阵列形成以四个微型电极为单元进行生物阻抗测量的多个检测区域,所述多个检测区域包括以靠近中心处的四个微型电极进行生物阻抗测量所覆盖待测生物组织而形成的最小检测区域,每个检测区域以最小检测区域为中心互相重叠。An array type biological tissue impedance measuring probe comprises a substrate and an electrode array embedded on the substrate, the electrode array forming a plurality of detection regions for performing bioimpedance measurement in units of four microelectrodes, the plurality of detections The area includes a minimum detection area formed by biological impedance measurement covering the biological tissue to be tested with four microelectrodes near the center, and each detection area overlaps with the smallest detection area as a center.
优选的,所述电极阵列是由呈中心对称分布的多个微型电极排列而成。
Preferably, the electrode array is formed by arranging a plurality of micro electrodes symmetrically distributed in a center.
优选的,所述每个检测区域的四个微型电极为分别位于方形的四个角上。Preferably, the four micro electrodes of each detection area are respectively located at four corners of the square.
优选的,所述电极阵列由多个以最小检测区域的四个微型电极的对角线两端延伸分布的电极排列而成。Preferably, the electrode array is formed by arranging a plurality of electrodes extending at both ends of a diagonal line of four microelectrodes of a minimum detection area.
进一步的,所述每个检测区域的四个微型电极有任意相邻两微型电极为激励电极对,另外任意相邻两微型电极为采集电极对。Further, the four microelectrodes of each detection region have any two adjacent microelectrodes as excitation electrode pairs, and any two adjacent microelectrodes are collection electrode pairs.
优选的,还包括激励单元、切换单元以及采集信号单元,其中所述探头通过切换单元分别与所述激励单元和所述采集信号单元连接。Preferably, the method further includes an excitation unit, a switching unit, and an acquisition signal unit, wherein the probe is respectively connected to the excitation unit and the acquisition signal unit through a switching unit.
进一步优选的,所述切换单元为多选一开关或由多个多选一开关构成的开关阵列。Further preferably, the switching unit is a multi-select switch or a switch array composed of a plurality of multiple-select switches.
为了实现上述目的,本发明所采用的又一技术方案如下:In order to achieve the above object, another technical solution adopted by the present invention is as follows:
一种阵列式生物组织阻抗测量探头,包括基板和嵌于所述基板上的电极阵列,所述电极阵列是由多个电极均匀分布而成,并且以四个电极为单元均匀分布形成用于生物阻抗测量的多个检测区域;所述多个检测区域包括以靠近中心处的四个电极在生物阻抗测量过程中所覆盖待测生物组织而形成的最小检测区域,每个检测区域以最小检测区域为中心互相重叠而成。An array type biological tissue impedance measuring probe comprises a substrate and an electrode array embedded on the substrate, wherein the electrode array is uniformly distributed by a plurality of electrodes, and is uniformly distributed in units of four electrodes for use in living organisms a plurality of detection regions of the impedance measurement; the plurality of detection regions include a minimum detection region formed by covering the biological tissue to be tested during bioimpedance measurement with four electrodes near the center, and each detection region has a minimum detection region The centers are overlapped with each other.
优选的,所述电极阵列是由呈中心对称分布的多个电极排列而成。Preferably, the electrode array is formed by arranging a plurality of electrodes symmetrically distributed in a center.
优选的,所述每个检测区域的四个电极为分别位于方形的四个角上。Preferably, the four electrodes of each detection area are respectively located at four corners of the square.
进一步优选的,所述电极阵列由多个以最小检测区域的四个电极的对角线两端延伸分布的电极排列而成。Further preferably, the electrode array is formed by arranging a plurality of electrodes extending at both ends of a diagonal of four electrodes of a minimum detection area.
优选的,所述每个检测区域的四个电极有任意相邻两电极为激励电极对,另外任意相邻两电极为采集电极对。Preferably, the four electrodes of each detection area have any adjacent two electrodes as excitation electrode pairs, and any adjacent two electrodes are collection electrode pairs.
优选的,还包括激励单元、切换单元以及采集信号单元,其中所述探头通过切换单元分别与所述激励单元和所述采集信号单元连接。Preferably, the method further includes an excitation unit, a switching unit, and an acquisition signal unit, wherein the probe is respectively connected to the excitation unit and the acquisition signal unit through a switching unit.
进一步优选的,所述切换单元为多选一开关或由多个多选一开关构成的开关阵列。Further preferably, the switching unit is a multi-select switch or a switch array composed of a plurality of multiple-select switches.
为了实现上述目的,本发明所采用的又一技术方案如下:
In order to achieve the above object, another technical solution adopted by the present invention is as follows:
一种使用上述技术方案中所述测量探头的阵列式生物阻抗测量方法,所述测量探头与待测生物组织的接触面通过切换单元切换控制电极阵列形成由四个微型电极检测而成的多个检测区域,其中该测量方法的具体步骤如下:An array type bio-impedance measuring method using the measuring probe described in the above technical solution, wherein a contact surface of the measuring probe and the biological tissue to be tested is switched by the switching unit to form a plurality of micro-electrodes detected by the switching unit The detection area, wherein the specific steps of the measurement method are as follows:
S1、将测量探头与待测生物组织接触;S1, contacting the measuring probe with the biological tissue to be tested;
S2、根据覆盖待测组织面积的大小依次由大到小逐个切换控制的每个检测区域的信号激励和信号采集,至最小检测区域后停止切换,并得出相对应检测区域的第一阻抗频谱特征参数集X(i),其中i=1、2、3…n;S2. According to the size of the area of the tissue to be tested, the signal excitation and signal acquisition of each detection area are switched from large to small, and the switching is stopped after the minimum detection area, and the first impedance spectrum of the corresponding detection area is obtained. Characteristic parameter set X(i), where i=1, 2, 3...n;
S3、将所检测的第一阻抗频谱特征参数集X(i)与临床生物组织阻抗数据库相关组织阻抗特征进行对照;S3. Comparing the detected first impedance spectral characteristic parameter set X(i) with the tissue impedance characteristic of the clinical biological tissue impedance database;
S4、根据对照结果是否有符合某一组织阻抗特征所对应的检测区域与另一检测区域有交集来验证待测生物组织阻抗频谱特征参数的准确性。S4. According to the comparison result, whether the detection area corresponding to a certain tissue impedance characteristic has an intersection with another detection area to verify the accuracy of the impedance spectrum characteristic parameter of the biological tissue to be tested.
本发明采用上述所述电极阵列排布以及切换控制电极信号激励和信号采集,通过改变检测待测生物组织的实际检测覆盖面,实现对待测生物组织不同深度和面积的全方位无死角检测,从而有效避免漏检或错检;同时也有利于辅助待测生物组织定位所测阻抗特征参数中某一组织的位置;又通过切换对待测生物组织不同尺度的检测区域检测,对照所测阻抗频谱特征参数是否符合生物组织阻抗频谱数据库相关组织特征,相比现有技术提高检测效率、降低漏检率,同时还对某一组织进行较精确的定位。本发明尤其适合较小的待测生物组织样本检测。尤其适合较小的待测生物组织进行阵列式的微观和宏观分析。The invention adopts the above-mentioned electrode array arrangement and switching control electrode signal excitation and signal acquisition, and realizes the omnidirectional no-dead detection of different depths and areas of the biological tissue to be tested by changing the actual detection coverage of the biological tissue to be tested, thereby effectively Avoid missed detection or misdetection; at the same time, it is also helpful to assist the biological tissue to be measured to locate the position of a certain tissue in the impedance characteristic parameter; and to switch the detection area detection of different scales of the biological tissue to be tested, and compare the measured impedance spectral characteristic parameters Whether it conforms to the relevant organizational characteristics of the biological tissue impedance spectrum database, compared with the prior art, the detection efficiency is improved, the missed detection rate is lowered, and a certain organization is more accurately positioned. The invention is particularly suitable for the detection of small biological tissue samples to be tested. It is especially suitable for array microscopic and macroscopic analysis of smaller biological tissues to be tested.
下面结合附图和实施例对本发明进一步说明:The present invention is further described below in conjunction with the accompanying drawings and embodiments:
图1是本发明实施例一所述测量探头的电极阵列分布示意图;1 is a schematic view showing an electrode array distribution of a measuring probe according to Embodiment 1 of the present invention;
图2是本发明实施例一所述测量探头的结构示意图;2 is a schematic structural view of a measuring probe according to Embodiment 1 of the present invention;
图3是本发明实施例一所述检测区域四个微型电极工作示意图;3 is a schematic view showing the operation of four microelectrodes in the detection area according to the first embodiment of the present invention;
图4是本发明实施例二所述测量探头的电极阵列分布示意图;4 is a schematic diagram showing an electrode array distribution of a measuring probe according to Embodiment 2 of the present invention;
图5是本发明实施例三所述测量方法的基本流程示意图;
5 is a schematic diagram of a basic flow of a measurement method according to Embodiment 3 of the present invention;
图6是本发明假定待测生物组织内的组织P1分布示意图一;Figure 6 is a schematic view of the distribution of tissue P1 in the biological tissue to be tested according to the present invention;
图7是本发明假定待测生物组织内的组织P1分布示意图二。Fig. 7 is a schematic view showing the distribution of tissue P1 in the biological tissue to be tested according to the present invention.
附图标记:Reference mark:
10-测量探头,11-基板,12-电极阵列,121-微型电极,13-激励单元,14-切换单元,15-采集信号单元;20-生物组织;P1-组织;10-measurement probe, 11-substrate, 12-electrode array, 121-microelectrode, 13-excitation unit, 14-switching unit, 15-acquisition signal unit; 20-biological tissue; P1-tissue;
E1、E1′、E2、E2′、E3、E3′、E4、E4′、E5、E5′、E6、E6′、E7、E7′、E8、E8′、E9、E9′、E10、E10′、E11、E11′、E12、E12′-微型电极。E1, E1', E2, E2', E3, E3', E4, E4', E5, E5', E6, E6', E7, E7', E8, E8', E9, E9', E10, E10', E11, E11', E12, E12'-microelectrodes.
现有人们通常采用固定电极头尺寸的四电极阻抗或双电极阻抗测量方案。而该现有技术方案存在电极尺寸和排布方式固定,且仅对特定尺寸和深度内的检测目标敏感,分辨率有限;而且还需要在待测生物组织上选点并多次测量,导致对于待测生物组织的测量效率低,且容易漏检或误测。为此,本发明提出一种阵列式生物组织阻抗测量探头及其测量方法。以下所述仅为本发明的较佳实施例,并不因此而限定本发明的保护范围。A four-electrode impedance or two-electrode impedance measurement scheme with a fixed electrode tip size is commonly used. However, the prior art solution has a fixed electrode size and arrangement, and is sensitive only to detection targets within a specific size and depth, and has limited resolution; and it is also required to select points on the biological tissue to be tested and measure multiple times, resulting in The measurement of the biological tissue to be tested is low, and it is easy to miss or misdetect. To this end, the present invention provides an array type biological tissue impedance measuring probe and a measuring method thereof. The following description is only a preferred embodiment of the invention and is not intended to limit the scope of the invention.
实施例一Embodiment 1
如图1所示,本发明实施例提出一种阵列式生物组织阻抗测量探头10,包括基板11和嵌于所述基板11上的电极阵列12,所述电极阵列形成12以四个微型电极E1、E2、E3、E4(E5、E6、E7、E8或E9、E10、E11、E12)为单元进行生物阻抗测量的多个检测区域E1E2E3E4、E5E6E7E8、E9E10E11E12,所述多个检测区域包括以靠近中心处的四个微型电极E1、E2、E3、E4进行生物阻抗测量所覆盖待测生物组织而形成的最小检测区域,每个检测区域E1E2E3E4、E5E6E7E8、E9E10E11E12以最小检测区域E1E2E3E4为中心互相重叠。其中所述电极阵列12是由呈中心对称分布的多个微型电极E1、E2、……En排列而成。As shown in FIG. 1 , an embodiment of the present invention provides an array type biological tissue impedance measuring probe 10 including a substrate 11 and an electrode array 12 embedded on the substrate 11. The electrode array is formed by 12 with four micro electrodes E. 1 , E 2 , E 3 , E 4 (E 5 , E 6 , E 7 , E 8 or E 9 , E 10 , E 11 , E 12 ) are a plurality of detection regions E 1 E 2 for bioimpedance measurement of the unit E 3 E 4 , E 5 E 6 E 7 E 8 , E 9 E 10 E 11 E 12 , the plurality of detection regions include four microelectrodes E 1 , E 2 , E 3 , E 4 near the center Performing a minimum detection area formed by bioimpedance measurement covering the biological tissue to be tested, and each detection area E 1 E 2 E 3 E 4 , E 5 E 6 E 7 E 8 , E 9 E 10 E 11 E 12 with minimum detection The regions E 1 E 2 E 3 E 4 are centered on each other. Wherein the plurality of micro-electrodes of the electrode array 12 is a centrosymmetric distribution E 1, E 2, ...... E n arrayed.
在本发明实施例中,所述每个检测区域E1E2E3E4、E5E6E7E8、E9E10E11E12的四个微型电极E1、E2、E3、E4(E5、E6、E7、E8或E9、E10、E11、E12)为分别位于方形的四个角上。所述相邻电极间间距小于P或大于等于2P,其中P为2mm。
In the embodiment of the present invention, the four microelectrodes E 1 , E 2 of each of the detection regions E 1 E 2 E 3 E 4 , E 5 E 6 E 7 E 8 , E 9 E 10 E 11 E 12 , E 3 , E 4 (E 5 , E 6 , E 7 , E 8 or E 9 , E 10 , E 11 , E 12 ) are respectively located at the four corners of the square. The spacing between adjacent electrodes is less than P or greater than or equal to 2P, where P is 2 mm.
如图2所示,本发明实施例所述测量探头还包括激励单元13、切换单元14以及采集信号单元15,其中所述探头10通过切换单元14分别与所述激励单元13和所述采集信号单元15连接。As shown in FIG. 2, the measurement probe according to the embodiment of the present invention further includes an excitation unit 13, a switching unit 14, and an acquisition signal unit 15, wherein the probe 10 and the excitation unit 13 and the acquisition signal are respectively passed through the switching unit 14. Unit 15 is connected.
如图3所示,在本发明实施例中所述每个检测区域的四个微型电极有任意相邻两微型电极为激励电极对,另外任意相邻两微型电极为采集电极对,其中所述激励电极对施加激励正负信号,所述采集电极对负责采集被激励目标(待测生物组织)上的电压信号或电流信号;通过切换单元14切换控制使采集电极对覆盖每组相邻电极并分别通过信号采集进行生物阻抗测量,从而达到对待测生物组织全方位的生物阻抗测量。本发明实施例所述切换单元14为多选一开关或由多个多选一开关构成的开关阵列。As shown in FIG. 3, in the embodiment of the present invention, the four microelectrodes of each detection region have any two adjacent microelectrodes as excitation electrode pairs, and any adjacent two microelectrodes are collection electrode pairs, wherein The excitation electrode pair applies an excitation positive and negative signal, and the collection electrode pair is responsible for collecting a voltage signal or a current signal on the excited target (the biological tissue to be tested); switching control by the switching unit 14 causes the collection electrode pair to cover each group of adjacent electrodes and The bioimpedance measurement is performed by signal acquisition to achieve a full range of bioimpedance measurement of the biological tissue to be measured. The switching unit 14 in the embodiment of the present invention is a switch array composed of multiple switches or multiple switches.
实施例二Embodiment 2
如图4所示,本发明实施例提出一种阵列式生物组织阻抗测量探头10,包括基板11和嵌于所述基板11上的电极阵列12,所述电极阵列形成12以四个微型电极E1′、E2′、E3′、E4′(E5′、E6′、E7′、E8′或E9′、E10′、E11′、E12′)为单元进行生物阻抗测量的多个检测区域E1′E2′E3′E4′、E5′E6′E7′E8′、E9′E10′E11′E12′,所述多个检测区域包括以靠近中心处的四个微型电极E1′、E2′、E3′、E4′进行生物阻抗测量所覆盖待测生物组织而形成的最小检测区域E1′E2′E3′E4′,每个检测区域E1′E2′E3′E4′、E5′E6′E7′E8′、E9′E10′E11′E12′以最小检测区域E1′E2′E3′E4′为中心互相重叠。其中所述电极阵列12是由呈中心对称分布的多个微型电极E1、E2、……En排列而成。As shown in FIG. 4, an embodiment of the present invention provides an array type biological tissue impedance measuring probe 10, comprising a substrate 11 and an electrode array 12 embedded on the substrate 11, the electrode array forming 12 with four micro electrodes E 1 ', E 2 ', E 3 ', E 4 ' (E 5 ', E 6 ', E 7 ', E 8 ' or E 9 ', E 10 ', E 11 ', E 12 ') A plurality of detection areas E 1 'E 2 'E 3 'E 4 ', E 5 'E 6 'E 7 'E 8 ', E 9 'E 10 'E 11 'E 12 ', measured by bioimpedance, said The detection area includes a minimum detection area E 1 'E 2 ' formed by biological impedance measurement covering the biological tissue to be tested with four microelectrodes E 1 ', E 2 ', E 3 ', and E 4 ' near the center. E 3 'E 4 ', each detection area E 1 'E 2 'E 3 'E 4 ', E 5 'E 6 'E 7 'E 8 ', E 9 'E 10 'E 11 'E 12 ' The minimum detection areas E 1 'E 2 'E 3 'E 4 ' are centered on each other. Wherein the plurality of micro-electrodes of the electrode array 12 is a centrosymmetric distribution E 1, E 2, ...... E n arrayed.
在本发明实施例中,所述每个检测区域E1′E2′E3′E4′、E5′E6′E7′E8′、E9′E10′E11′E12′的四个微型电极E1′、E2′、E3′、E4′(E5′、E6′、E7′、E8′或E9′、E10′、E11′、E12′)为分别位于方形的四个角上。所述电极阵列12由多个以最小检测区域的四个微型电极的对角线E1′E3′或E2′E4′两端延伸分布的电极E1′、E2′、……En′排列而成。In the embodiment of the present invention, each of the detection areas E 1 'E 2 'E 3 'E 4 ', E 5 'E 6 'E 7 'E 8 ', E 9 'E 10 'E 11 'E 12 'four microelectrodes E 1 ', E 2 ', E 3 ', E 4 ' (E 5 ', E 6 ', E 7 ', E 8 ' or E 9 ', E 10 ', E 11 ', E 12 ') are located at the four corners of the square. The electrode array 12 is composed of a plurality of electrodes E 1 ', E 2 ', and ... extending across the diagonal line E 1 'E 3 ' or E 2 'E 4 ' of the four microelectrodes of the smallest detection area. E n ' is arranged.
如如图2所示,本发明实施例所述测量探头还包括激励单元13、切换单元14以及采集信号单元15,其中所述探头10通过切换单元14分别与所述激励单
元13和所述采集信号单元15连接。本发明实施例所述切换单元14为多选一开关或由多个多选一开关构成的开关阵列。在本发明实施例中所述每个检测区域的四个微型电极的激励和采集方式均与图3所示工作模式相同,在此不再赘述。As shown in FIG. 2, the measurement probe according to the embodiment of the present invention further includes an excitation unit 13, a switching unit 14, and an acquisition signal unit 15, wherein the probe 10 and the excitation unit are respectively connected by the switching unit 14.
The element 13 is connected to the acquisition signal unit 15. The switching unit 14 in the embodiment of the present invention is a switch array composed of multiple switches or multiple switches. In the embodiment of the present invention, the excitation and collection modes of the four micro-electrodes of each detection area are the same as those shown in FIG. 3, and details are not described herein again.
本发明所述实施例一和实施例二,采用上述所述电极阵列排布以及切换控制电极信号激励和信号采集,通过改变检测待测生物组织的实际检测覆盖面,实现对待测生物组织不同深度和面积的全方位无死角检测,从而有效避免漏检或错检;同时也有利于辅助待测生物组织定位所测阻抗特征参数中某一组织的位置。尤其适合较小的待测生物组织进行阵列式的微观和宏观分析。In the first embodiment and the second embodiment of the present invention, the electrode array arrangement and the switching control electrode signal excitation and signal acquisition are adopted, and the actual detection coverage of the biological tissue to be tested is changed to realize different depths of the biological tissue to be tested and The area has no dead angle detection, which effectively avoids missed detection or misdetection; it also helps to locate the position of an organization in the measured impedance characteristic parameters of the biological tissue to be tested. It is especially suitable for array microscopic and macroscopic analysis of smaller biological tissues to be tested.
实施例三Embodiment 3
如图5所示,为了提高测量的效率、降低漏检率以及提高测量有效性,本发明实施例提出了一种使用上述技术方案中所述测量探头的阵列式生物阻抗测量方法,所述测量探头与待测生物组织的接触面通过切换单元切换控制电极阵列形成由四个微型电极检测而成的多个检测区域,其中该测量方法的具体步骤如下:As shown in FIG. 5, in order to improve the efficiency of measurement, reduce the rate of missed detection, and improve the effectiveness of measurement, an embodiment of the present invention provides an array type bio-impedance measurement method using the measurement probe described in the above technical solution, the measurement The contact surface of the probe and the biological tissue to be tested forms a plurality of detection regions detected by the four micro electrodes through the switching unit switching control electrode array, wherein the specific steps of the measurement method are as follows:
S1、将测量探头与待测生物组织接触;S1, contacting the measuring probe with the biological tissue to be tested;
S2、根据覆盖待测组织面积的大小依次由大到小逐个切换控制的每个检测区域的信号激励和信号采集,至最小检测区域后停止切换,并得出相对应检测区域的第一阻抗频谱特征参数集X(i),其中i=1、2、3…n,其中所述最小检测区域E1E2E3E4为靠近中心处的四个电极E1、E2、E3、E4构成的电极组检测所覆盖的区域;S2. According to the size of the area of the tissue to be tested, the signal excitation and signal acquisition of each detection area are switched from large to small, and the switching is stopped after the minimum detection area, and the first impedance spectrum of the corresponding detection area is obtained. Characteristic parameter set X(i), where i=1, 2, 3...n, wherein the minimum detection area E 1 E 2 E 3 E 4 is four electrodes E 1 , E 2 , E 3 near the center, The electrode group formed by E 4 detects the covered area;
S3、将所检测的第一阻抗频谱特征参数集X(i)与临床生物组织阻抗数据库相关组织阻抗特征进行对照,其中所述临床生物组织阻抗数据库为根据临床实验获取异常组织的阻抗频谱特征参数而建立数据库;S3. Comparing the detected first impedance spectral characteristic parameter set X(i) with a tissue impedance characteristic of a clinical biological tissue impedance database, wherein the clinical biological tissue impedance database is an impedance spectrum characteristic parameter obtained by acquiring an abnormal tissue according to a clinical experiment. And establish a database;
S4、根据对照结果是否有符合某一组织阻抗特征所对应的检测区域与另一检测区域有交集来验证待测生物组织阻抗频谱特征参数的准确性。S4. According to the comparison result, whether the detection area corresponding to a certain tissue impedance characteristic has an intersection with another detection area to verify the accuracy of the impedance spectrum characteristic parameter of the biological tissue to be tested.
如图6所示,本发明实施例所述测量方法具体为先将本发明实施例一中所
述的测量探头10与待测生物组织接触;其中覆盖待测组织检测区域为E1E2E3E4、E5E6E7E8和E9E10E11E12且每个检测区域的面积关系为
通过切换单元根据检测区域面积依次由大到小逐个切换控制的每个检测区域第一阻抗频谱特征参数X(1)、X(2)和X(3),至最小检测区域E1E2E3E4后停止切换,其中X(1)为检测区域E1E2E3E4的第一阻抗频谱特征参数,X(2)为检测区域E5E6E7E8的第一阻抗频谱特征参数,X(3)为检测区域E9E10E11E12的第一阻抗频谱特征参数,所以检测所得第一阻抗频谱特征参数集X(2),X(3)};将所检测的第一阻抗频谱特征参数X(1)、X(2)和X(3)分别与临床生物组织阻抗数据库进行对照。根据对照结果判断是否有符合某一组织阻抗特征所对应的检测区域与另一检测区域有交集来验证待测生物组织阻抗频谱特征参数的准确性。As shown in FIG. 6 , the measurement method in the embodiment of the present invention is specifically to first contact the measurement probe 10 according to the first embodiment of the present invention with the biological tissue to be tested; wherein the detection area of the tissue to be tested is E 1 E 2 E 3 E 4 , E 5 E 6 E 7 E 8 and E 9 E 10 E 11 E 12 and the area relationship of each detection area is The first impedance spectral characteristic parameters X(1), X(2), and X(3) of each detection region that are controlled by the switching unit are sequentially switched from large to small according to the area of the detection area, to the minimum detection area E 1 E 2 E 3 E 4 stops switching, where X(1) is the first impedance spectral characteristic parameter of detection area E 1 E 2 E 3 E 4 and X(2) is the first impedance of detection area E 5 E 6 E 7 E 8 The spectral characteristic parameter, X(3) is the first impedance spectral characteristic parameter of the detection region E 9 E 10 E 11 E 12 , so the obtained first impedance spectral characteristic parameter set is detected. X(2), X(3)}; the detected first impedance spectral characteristic parameters X(1), X(2), and X(3) are respectively compared with a clinical biological tissue impedance database. According to the comparison result, it is judged whether there is an intersection of the detection area corresponding to a certain tissue impedance characteristic and another detection area to verify the accuracy of the impedance spectrum characteristic parameter of the biological tissue to be tested.
继续图6所示,根据比对分析结果确定待测生物组织内所对应的检测区域E1E2E3E4和E5E6E7E8的X(1)和X(2)存符合组织P1阻抗特征。根据比对分析结果确定覆盖待测生物组织存在组织P1的检测区域为E1E2E3E4和E5E6E7E8,经统计所述检测区域中具有重合交集的检测区域有两个,故验证所测待测生物组织阻抗频谱特征参数是准确性。Continuing with FIG. 6, determining X(1) and X(2) of the corresponding detection regions E 1 E 2 E 3 E 4 and E 5 E 6 E 7 E 8 in the biological tissue to be tested according to the comparison analysis result Meets the organization's P1 impedance characteristics. According to the result of the comparison analysis, it is determined that the detection areas covering the tissue P1 in the biological tissue to be tested are E 1 E 2 E 3 E 4 and E 5 E 6 E 7 E 8 , and the detection areas having the coincident intersection in the detection area are statistically detected. Two, so verify that the measured characteristic parameters of the measured biological tissue impedance are accurate.
同时根据每个检测区域所检测的阻抗频谱特征参数符合某一组织阻抗特征来定位该组织的在待测生物组织内的位置,比如由于检测区域E9E10E11E12所覆盖的待测生物组织区域相对组织P1所被覆盖的区域很小,可能检测不到符合组织P1的阻抗特征,而检测区域E1E2E3E4和E5E6E7E8符合组织P1的阻抗特征,则定位该组织P1存在于检测区域E1E2E3E4或E5E6E7E8内。At the same time, according to the impedance spectrum characteristic parameter detected by each detection area, the position of the tissue in the biological tissue to be tested is located according to a certain tissue impedance characteristic, for example, the test area covered by the detection area E 9 E 10 E 11 E 12 The area covered by the biological tissue area relative to the tissue P1 is small, and the impedance characteristics conforming to the tissue P1 may not be detected, and the detection areas E 1 E 2 E 3 E 4 and E 5 E 6 E 7 E 8 conform to the impedance of the tissue P1. Characteristic, the tissue P1 is located in the detection area E 1 E 2 E 3 E 4 or E 5 E 6 E 7 E 8 .
如图7所示,本发明实施例所述测量方法具体为先将本发明实施例二中所述的测量探头10与待测生物组织接触;其中覆盖待测组织检测区域为E1′E2′E3′E4′、E5′E6′、E7′E8′和E9′E10′E11′E12′且每个检测区域的面积关系为SE1′E2′E3′E4′<SE5′E6′E7′E8′<SE9′E10′E11′E12′,通过切换单元根据检测区域面积依次由大到小逐个切换控制的每个检测区域第一阻抗频谱特征参数X(1)、X(2)和X(3),至最小
检测区域E1′E2′E3′E4′后停止切换,其中X(1)为检测区域E1′E2′E3′E4′的第一阻抗频谱特征参数,X(2)为检测区域E5′E6′、E7′E8′的第一阻抗频谱特征参数,X(3)为检测区域E9′E10′E11′E12′的第一阻抗频谱特征参数,所以检测所得第一阻抗频谱特征参数集X(2),X(3)};将所检测的第一阻抗频谱特征参数X(1)、X(2)和X(3)分别与临床生物组织阻抗数据库进行比对对照。根据对照结果判断是否有符合某一组织阻抗特征所对应的检测区域与另一检测区域有交集来验证待测生物组织阻抗频谱特征参数的准确性。As shown in FIG. 7 , the measurement method in the embodiment of the present invention is specifically to first contact the measurement probe 10 described in the second embodiment of the present invention with the biological tissue to be tested; wherein the detection area of the tissue to be tested is E 1 'E 2 'E 3 'E 4 ', E 5 'E 6 ', E 7 'E 8 ' and E 9 'E 10 'E 11 'E 12 ' and the area relationship of each detection area is S E1 'E2'E3'E4'<SE5'E6'E7'E8'<SE9'E10'E11'E12' , the first impedance spectral characteristic parameter X of each detection area controlled by the switching unit according to the area of the detection area in turn from large to small (1), X(2) and X(3), stopping switching after the minimum detection area E 1 'E 2 'E 3 'E 4 ', where X(1) is the detection area E 1 'E 2 'E 3 The first impedance spectral characteristic parameter of 'E 4 ', X(2) is the first impedance spectral characteristic parameter of the detection regions E 5 'E 6 ', E 7 'E 8 ', and X(3) is the detection region E 9 ' The first impedance spectral characteristic parameter of E 10 'E 11 'E 12 ', so the obtained first impedance spectral characteristic parameter set is detected X(2), X(3)}; the detected first impedance spectral characteristic parameters X(1), X(2), and X(3) are compared with the clinical biological tissue impedance database, respectively. According to the comparison result, it is judged whether there is an intersection of the detection area corresponding to a certain tissue impedance characteristic and another detection area to verify the accuracy of the impedance spectrum characteristic parameter of the biological tissue to be tested.
继续图7所示,根据比对分析结果确定待测生物组织内X(1)、X(2)和X(3)所对应的检测区域E1′E2′E3′E4′和E5′E6′E7′E8″存在组织P1阻抗特征。根据比对分析结果确定覆盖待测生物组织符合组织P1阻抗特征所对应的检测区域为E1′E2′E3′E4′和E5′E6′E7′E8′,经统计所述检测区域中具有重合交集的检测区域有两个,故验证所测待测生物组织阻抗频谱特征参数是准确性。Continuing with FIG. 7, the detection regions E 1 'E 2 'E 3 'E 4 ' and E corresponding to X(1), X(2), and X(3) in the biological tissue to be tested are determined based on the results of the comparison analysis. 5 'E 6 'E 7 'E 8 ′′ has a tissue P1 impedance characteristic. According to the result of the comparison analysis, it is determined that the detection area corresponding to the tissue P1 impedance characteristic covering the biological tissue to be tested is E 1 'E 2 'E 3 'E 4 ' and E 5 'E 6 'E 7 'E 8 ', there are two detection areas with coincident intersections in the detection area by statistics, so it is verified that the measured impedance characteristic parameters of the biological tissue to be tested are accurate.
同时根据每个检测区域所检测的阻抗频谱特征参数符合某一组织阻抗特征来定位该组织的在待测生物组织内的位置,比如由于检测区域E9′E10′E11′E12′所覆盖的待测生物组织区域相对组织P1所被覆盖的区域很小,可能检测不到符合组织P1的阻抗特征,而检测区域E1′E2′E3′E4′和E5′E6′E7′E8′符合组织P1的阻抗特征,则定位该组织P1存在于检测区域E1′E2′E3′E4′或E5′E6′E7′E8′内。At the same time, according to the impedance spectrum characteristic parameter detected by each detection area, the position of the tissue in the biological tissue to be tested is located according to a certain tissue impedance characteristic, for example, due to the detection area E 9 'E 10 'E 11 'E 12 ' The area of the covered biological tissue to be covered is small relative to the area covered by the tissue P1, and the impedance characteristics conforming to the tissue P1 may not be detected, and the detection areas E 1 'E 2 'E 3 'E 4 ' and E 5 'E 6 'E 7 'E 8 ' conforms to the impedance characteristic of tissue P1, and the tissue P1 is located in the detection area E 1 'E 2 'E 3 'E 4 ' or E 5 'E 6 'E 7 'E 8 '.
本发明实施例三和实施例四所述的阵列式生物阻抗组织测量方法根据采用多组微型生物阻抗检测电极组成检测阵列以及结合统计分析处理方式,高效获得整个待测生物组织的各个检测区域的微观阻抗谱特征,并通过与临床生物组织阻抗数据库进行比对分析,以及统计验证后,确认待生物组织内是否异常组织。同时本发明还可以通过分析检测区域间的阻抗频谱特征,精确定位异常组织的位置。其中本发明实施例三相比较本发明实施例四在同一阵列式电极接触面能分布排列同种类型的电极,节省了电极排布所占用的空间。The array type bioimpedance tissue measuring method according to the third embodiment and the fourth embodiment of the present invention can efficiently obtain the detection areas of the whole biological tissue to be tested according to the detection array of multiple sets of micro bio-impedance detecting electrodes and the statistical analysis processing method. Microscopic impedance spectrum characteristics, and through the comparative analysis with the clinical biological tissue impedance database, and statistical verification, confirm whether abnormal tissue in the biological tissue. At the same time, the invention can accurately locate the position of the abnormal tissue by analyzing the impedance spectrum characteristics between the detection regions. In the embodiment of the present invention, the fourth embodiment of the present invention can distribute the same type of electrodes on the same array electrode contact surface, thereby saving the space occupied by the electrode arrangement.
上述内容仅为本发明的较佳实施例,对于本领域的普通技术人员,依据本发明的思想,在具体实施方式及应用范围上均会有改变之处,本说明书内容不
应理解为对本发明的限制。
The above is only a preferred embodiment of the present invention, and those skilled in the art will have any changes in the specific embodiments and application scope according to the idea of the present invention.
It should be understood that the invention is limited.
Claims (15)
- 一种阵列式生物组织阻抗测量探头,包括基板和嵌于所述基板上的电极阵列,所述电极阵列是由多个电极均匀分布而成并形成以四个电极为单元进行生物阻抗测量的多个检测区域,所述多个检测区域包括以靠近中心处的四个电极进行生物阻抗测量所覆盖待测生物组织而形成的最小检测区域,每个检测区域以最小检测区域为中心互相重叠。An array type biological tissue impedance measuring probe comprises a substrate and an electrode array embedded on the substrate, wherein the electrode array is uniformly distributed by a plurality of electrodes and forms a bioimpedance measurement by using four electrodes as a unit And a plurality of detection areas, wherein the plurality of detection areas comprise a minimum detection area formed by biological impedance measurement covering the biological tissue to be tested with four electrodes near the center, and each detection area overlaps with the smallest detection area as a center.
- 根据权利要求1所述的阵列式生物组织阻抗测量探头,其特征在于,所述电极阵列是由呈中心对称分布的多个电极排列而成。The array type biological tissue impedance measuring probe according to claim 1, wherein the electrode array is formed by arranging a plurality of electrodes symmetrically distributed in a center.
- 根据权利要求1所述的阵列式生物组织阻抗测量探头,其特征在于,所述每个检测区域的四个电极为分别位于方形的四个角上。The array type biological tissue impedance measuring probe according to claim 1, wherein the four electrodes of each of the detecting regions are respectively located at four corners of the square.
- 根据权利要求1所述的阵列式生物组织阻抗测量探头,其特征在于,所述电极阵列由多个以最小检测区域的四个电极的对角线两端延伸分布的电极排列而成。The array type biological tissue impedance measuring probe according to claim 1, wherein the electrode array is formed by arranging a plurality of electrodes extending at both ends of a diagonal line of four electrodes of a minimum detection area.
- 根据权利要求1所述的阵列式生物组织阻抗测量探头,其特征在于,所述每个检测区域的四个电极有任意相邻两电极为激励电极对,另外任意相邻两电极为采集电极对。The array type biological tissue impedance measuring probe according to claim 1, wherein any four electrodes of each of the detecting regions have any two adjacent electrodes as excitation electrode pairs, and any adjacent two electrodes are collecting electrode pairs. .
- 根据权利要求1所述的阵列式生物组织阻抗测量探头,其特征在于,还包括激励单元、切换单元以及采集信号单元,其中所述探头通过切换单元分别与所述激励单元和所述采集信号单元连接。The array type biological tissue impedance measuring probe according to claim 1, further comprising an excitation unit, a switching unit, and an acquisition signal unit, wherein the probe is respectively coupled to the excitation unit and the acquisition signal unit by a switching unit connection.
- 根据权利要求6所述的阵列式生物组织阻抗测量探头,其特征在于,所述切换单元为多选一开关或由多个多选一开关构成的开关阵列。The array type biological tissue impedance measuring probe according to claim 6, wherein the switching unit is a multi-select switch or a switch array composed of a plurality of multi-select switches.
- 一种阵列式生物组织阻抗测量探头,包括基板和嵌于所述基板上的电极阵列,所述电极阵列是由多个电极均匀分布而成,并且以四个电极为单元均匀分布形成用于生物阻抗测量的多个检测区域;所述多个检测区域包括以靠近中心处的四个电极在生物阻抗测量过程中所覆盖待测生物组织而形成的最小检测 区域,每个检测区域以最小检测区域为中心互相重叠而成。An array type biological tissue impedance measuring probe comprises a substrate and an electrode array embedded on the substrate, wherein the electrode array is uniformly distributed by a plurality of electrodes, and is uniformly distributed in units of four electrodes for use in living organisms a plurality of detection regions of impedance measurement; the plurality of detection regions including a minimum detection formed by biological electrodes covered by the four electrodes near the center during bioimpedance measurement In the area, each detection area is formed by overlapping the smallest detection area.
- 根据权利要求8所述的阵列式生物组织阻抗测量探头,其特征在于,所述电极阵列是由呈中心对称分布的多个电极排列而成。The array type biological tissue impedance measuring probe according to claim 8, wherein the electrode array is formed by arranging a plurality of electrodes symmetrically distributed in a center.
- 根据权利要求8所述的阵列式生物组织阻抗测量探头,其特征在于,所述每个检测区域的四个电极为分别位于方形的四个角上。The array type biological tissue impedance measuring probe according to claim 8, wherein the four electrodes of each of the detecting regions are respectively located at four corners of the square.
- 根据权利要求10所述的阵列式生物组织阻抗测量探头,其特征在于,所述电极阵列由多个以最小检测区域的四个电极的对角线两端延伸分布的电极排列而成。The array type biological tissue impedance measuring probe according to claim 10, wherein the electrode array is formed by arranging a plurality of electrodes extending at both ends of a diagonal line of four electrodes of a minimum detection area.
- 根据权利要求8所述的阵列式生物组织阻抗测量探头,其特征在于,所述每个检测区域的四个电极有任意相邻两电极为激励电极对,另外任意相邻两电极为采集电极对。The array type biological tissue impedance measuring probe according to claim 8, wherein each of the four electrodes of each detecting area has any adjacent two electrodes as excitation electrode pairs, and any adjacent two electrodes are collecting electrode pairs. .
- 根据权利要求8所述的阵列式生物组织阻抗测量探头,其特征在于,还包括激励单元、切换单元以及采集信号单元,其中所述探头通过切换单元分别与所述激励单元和所述采集信号单元连接。The array type biological tissue impedance measuring probe according to claim 8, further comprising an excitation unit, a switching unit, and an acquisition signal unit, wherein the probe is respectively coupled to the excitation unit and the acquisition signal unit by a switching unit connection.
- 根据权利要13所述的阵列式生物组织阻抗测量探头,其特征在于,所述切换单元为多选一开关或由多个多选一开关构成的开关阵列。The array type biological tissue impedance measuring probe according to claim 13, wherein the switching unit is a multi-selection switch or a switch array composed of a plurality of multi-select switches.
- 一种使用测量探头的阵列式生物阻抗测量方法,所述测量探头与待测生物组织的接触面通过切换开关单元切换控制电极阵列形成由四个电极检测而成的多个检测区域,其中该测量方法的具体步骤如下:An array type bio-impedance measuring method using a measuring probe, the measuring surface of the measuring probe and the biological tissue to be tested is switched by the switching switch unit to form a plurality of detecting regions detected by four electrodes, wherein the measuring The specific steps of the method are as follows:S1、将测量探头与待测生物组织接触;S1, contacting the measuring probe with the biological tissue to be tested;S2、根据覆盖待测组织面积的大小依次由大到小逐个切换控制的每个检测区域的信号激励和信号采集,至最小检测区域后停止切换,并得出相对应检测区域的第一阻抗频谱特征参数集X(i),其中i=1、2、3…n;S2. According to the size of the area of the tissue to be tested, the signal excitation and signal acquisition of each detection area are switched from large to small, and the switching is stopped after the minimum detection area, and the first impedance spectrum of the corresponding detection area is obtained. Characteristic parameter set X(i), where i=1, 2, 3...n;S3、将所检测的第一阻抗频谱特征参数集X(i)与临床生物组织阻抗数据库相关组织阻抗特征进行对照;S3. Comparing the detected first impedance spectral characteristic parameter set X(i) with the tissue impedance characteristic of the clinical biological tissue impedance database;S4、根据对照结果是否有符合某一组织阻抗特征所对应的检测区域与另一 检测区域有交集来验证待测生物组织阻抗频谱特征参数的准确性。 S4. According to the comparison result, whether there is a detection area corresponding to a certain tissue impedance characteristic and another The detection area has an intersection to verify the accuracy of the characteristic parameters of the impedance spectrum of the biological tissue to be tested.
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