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WO2012025637A9 - An agglutination assay method and device - Google Patents

An agglutination assay method and device Download PDF

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Publication number
WO2012025637A9
WO2012025637A9 PCT/EP2011/064841 EP2011064841W WO2012025637A9 WO 2012025637 A9 WO2012025637 A9 WO 2012025637A9 EP 2011064841 W EP2011064841 W EP 2011064841W WO 2012025637 A9 WO2012025637 A9 WO 2012025637A9
Authority
WO
WIPO (PCT)
Prior art keywords
lateral flow
agglutination
flow assay
sample
monitoring
Prior art date
Application number
PCT/EP2011/064841
Other languages
French (fr)
Other versions
WO2012025637A1 (en
Inventor
Anthony Joseph Killard
Nigel Kent
Magdalena Maria Dudek
Original Assignee
Dublin City University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dublin City University filed Critical Dublin City University
Publication of WO2012025637A1 publication Critical patent/WO2012025637A1/en
Publication of WO2012025637A9 publication Critical patent/WO2012025637A9/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/4905—Determining clotting time of blood
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/86—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
    • G01N2333/4609—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates from reptiles
    • G01N2333/4613—Snake venom

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Ecology (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention is directed to a method and device for monitoring and measuring agglutination within a sample using a lateral flow assay device. In particular, the present invention is directed to a method for monitoring and measuring the onset of agglutination within a sample on a lateral flow assay device comprising micro-projections to define a lateral flow assay path wherein the method comprises combining the sample with at least one detectable marker and at least one agglutination marker or a combination thereof to form a labelled sample; subjecting the labelled sample to lateral flow; and monitoring the redistribution of the agglutination marker through the lateral flow assay path as agglutination occurs. The invention is also directed to a lateral flow assay device for carrying out the method and a kit comprising the lateral flow assay device and detection means.
PCT/EP2011/064841 2010-08-27 2011-08-29 An agglutination assay method and device WO2012025637A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1014316.2A GB201014316D0 (en) 2010-08-27 2010-08-27 A agglutination assay method and device
GB1014316.2 2010-08-27

Publications (2)

Publication Number Publication Date
WO2012025637A1 WO2012025637A1 (en) 2012-03-01
WO2012025637A9 true WO2012025637A9 (en) 2012-05-18

Family

ID=43013346

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2011/064841 WO2012025637A1 (en) 2010-08-27 2011-08-29 An agglutination assay method and device

Country Status (2)

Country Link
GB (1) GB201014316D0 (en)
WO (1) WO2012025637A1 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012037266A2 (en) * 2010-09-14 2012-03-22 California Institute Of Technology Using blood clot in microfluidic valving applications
JP6359263B2 (en) * 2012-11-15 2018-07-18 オーソ・クリニカル・ダイアグノスティックス・インコーポレーテッド Quality / process control of lateral flow assay devices based on flow monitoring
TWI500921B (en) 2013-01-14 2015-09-21 Ind Tech Res Inst Optical sensing chip
US10073091B2 (en) 2014-08-08 2018-09-11 Ortho-Clinical Diagnostics, Inc. Lateral flow assay device
DE102015222787A1 (en) * 2015-11-18 2017-05-18 Siemens Healthcare Diagnostics Products Gmbh Platelet function analysis system
CA3135087A1 (en) * 2019-03-27 2020-10-01 Haemokinesis Limited Method and device for measuring the fibrinogen concentration in blood samples
CN113395582B (en) * 2020-03-12 2023-04-07 平湖莱顿光学仪器制造有限公司 Transmittance-related intelligent two-dimensional video playing method and video device thereof
CN111856042A (en) * 2020-07-31 2020-10-30 深圳微点生物技术股份有限公司 Device for detecting blood coagulation characteristics and application thereof
CN113740326B (en) * 2021-09-06 2022-04-29 中国人民解放军军事科学院军事医学研究院 Device and method for rapidly detecting concentration of fibrinogen in whole blood

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5469497A (en) 1977-11-12 1979-06-04 Kyoto Daiichi Kagaku Kk Method and device for measuring blood solidification time
US4963498A (en) 1985-08-05 1990-10-16 Biotrack Capillary flow device
US4849340A (en) 1987-04-03 1989-07-18 Cardiovascular Diagnostics, Inc. Reaction system element and method for performing prothrombin time assay
US5039617A (en) 1989-04-20 1991-08-13 Biotrack, Inc. Capillary flow device and method for measuring activated partial thromboplastin time
US5302348A (en) 1992-12-10 1994-04-12 Itc Corporation Blood coagulation time test apparatus and method
US5908786A (en) 1997-12-12 1999-06-01 Akzo Nobel, N.V. Blood coagulation monitoring device with liquid crystal and gradient heater
SE0201738D0 (en) 2002-06-07 2002-06-07 Aamic Ab Micro-fluid structures
SE0400662D0 (en) 2004-03-24 2004-03-24 Aamic Ab Assay device and method
SE528233C2 (en) 2005-06-20 2006-09-26 Aamic Ab Fluid handling device for handling fluid to be assayed, comprises absorbing zone(s) in fluid contact with second end of first passage and comprising projections perpendicular to substrate surface and capable of generating capillary flow
CN101720432A (en) * 2007-06-20 2010-06-02 Mec戴内米克公司 Methods and apparatus for measuring blood coagulation
US20090208975A1 (en) * 2007-12-13 2009-08-20 Beckman Coulter, Inc. Device and methods for detecting a target cell

Also Published As

Publication number Publication date
WO2012025637A1 (en) 2012-03-01
GB201014316D0 (en) 2010-10-13

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