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WO2011159050A3 - 인간 신경전구세포의 도파민 뉴우런으로의 분화방법 및 분화용 배지 - Google Patents

인간 신경전구세포의 도파민 뉴우런으로의 분화방법 및 분화용 배지 Download PDF

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Publication number
WO2011159050A3
WO2011159050A3 PCT/KR2011/004118 KR2011004118W WO2011159050A3 WO 2011159050 A3 WO2011159050 A3 WO 2011159050A3 KR 2011004118 W KR2011004118 W KR 2011004118W WO 2011159050 A3 WO2011159050 A3 WO 2011159050A3
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WIPO (PCT)
Prior art keywords
progenitor cells
neural progenitor
human neural
medium
differentiation
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Application number
PCT/KR2011/004118
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English (en)
French (fr)
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WO2011159050A2 (ko
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문지숙
Original Assignee
차의과학대학교 산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by 차의과학대학교 산학협력단 filed Critical 차의과학대학교 산학협력단
Priority to EP11795916.3A priority Critical patent/EP2581443B1/en
Priority to US13/704,030 priority patent/US8921107B2/en
Priority to CN201180029604.3A priority patent/CN102985535B/zh
Priority to ES11795916.3T priority patent/ES2532928T3/es
Priority to JP2013515258A priority patent/JP5776101B2/ja
Priority to CA2802661A priority patent/CA2802661C/en
Priority to AU2011265956A priority patent/AU2011265956B2/en
Publication of WO2011159050A2 publication Critical patent/WO2011159050A2/ko
Publication of WO2011159050A3 publication Critical patent/WO2011159050A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/01—Modulators of cAMP or cGMP, e.g. non-hydrolysable analogs, phosphodiesterase inhibitors, cholera toxin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/119—Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/41—Hedgehog proteins; Cyclopamine (inhibitor)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/80—Neurotransmitters; Neurohormones
    • C12N2501/815—Dopamine

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  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Neurology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Neurosurgery (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

본 발명은 인간 신경전구세포를 푸사린산(fusaric acid)을 포함하는 배지 중에서 배양하는 것을 포함하는, 인간 신경전구세포의 도파민 뉴우런으로의 분화방법을 제공한다. 또한, 본 발명은 인간 신경전구세포의 도파민 뉴우런으로의 분화용 배지를 제공한다.
PCT/KR2011/004118 2010-06-14 2011-06-07 인간 신경전구세포의 도파민 뉴우런으로의 분화방법 및 분화용 배지 WO2011159050A2 (ko)

Priority Applications (7)

Application Number Priority Date Filing Date Title
EP11795916.3A EP2581443B1 (en) 2010-06-14 2011-06-07 Method for differentiating human neural progenitor cells into dopaminergic neurons, and medium for differentiation thereof
US13/704,030 US8921107B2 (en) 2010-06-14 2011-06-07 Method for differentiating human neural progenitor cells into dopaminergic neurons, and medium for differentiation thereof
CN201180029604.3A CN102985535B (zh) 2010-06-14 2011-06-07 人类神经前体细胞向多巴胺神经元的分化方法及用于分化的培养基
ES11795916.3T ES2532928T3 (es) 2010-06-14 2011-06-07 Método para diferenciar células progenitoras neurales humanas en neuronas dopaminérgicas, y medio para la diferenciación de las mismas
JP2013515258A JP5776101B2 (ja) 2010-06-14 2011-06-07 ヒト神経前駆細胞のドーパミン性ニューロンへの分化方法及び分化用培地
CA2802661A CA2802661C (en) 2010-06-14 2011-06-07 Method for differentiating human neural progenitor cells into dopaminergic neurons, and medium for differentiation thereof
AU2011265956A AU2011265956B2 (en) 2010-06-14 2011-06-07 Method for differentiating human neural progenitor cells into dopaminergic neurons, and medium for differentiation thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020100055832A KR101106020B1 (ko) 2010-06-14 2010-06-14 인간 신경전구세포의 도파민 뉴우런으로의 분화방법 및 분화용 배지
KR10-2010-0055832 2010-06-14

Publications (2)

Publication Number Publication Date
WO2011159050A2 WO2011159050A2 (ko) 2011-12-22
WO2011159050A3 true WO2011159050A3 (ko) 2012-05-03

Family

ID=45348710

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2011/004118 WO2011159050A2 (ko) 2010-06-14 2011-06-07 인간 신경전구세포의 도파민 뉴우런으로의 분화방법 및 분화용 배지

Country Status (9)

Country Link
US (1) US8921107B2 (ko)
EP (1) EP2581443B1 (ko)
JP (1) JP5776101B2 (ko)
KR (1) KR101106020B1 (ko)
CN (1) CN102985535B (ko)
AU (1) AU2011265956B2 (ko)
CA (1) CA2802661C (ko)
ES (1) ES2532928T3 (ko)
WO (1) WO2011159050A2 (ko)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6419073B2 (ja) * 2013-08-06 2018-11-07 武田薬品工業株式会社 ドパミン神経細胞の製造方法
CN104031882B (zh) * 2014-06-20 2017-03-01 上海安集协康生物技术股份有限公司 人神经干细胞体外定向诱导分化为多巴胺能神经元的方法
CN105483175A (zh) * 2016-01-21 2016-04-13 西北农林科技大学 一种禾谷镰刀菌脱氧雪腐镰刀菌烯醇合成的诱导物及方法
KR101783977B1 (ko) 2016-12-30 2017-10-10 제일약품주식회사 기형종 형성이 억제된 전분화능 줄기세포 유래 신경 전구체구의 제조 방법
KR102744286B1 (ko) * 2020-08-24 2024-12-19 차의과학대학교 산학협력단 신경줄기세포의 도파민 신경세포로의 분화 촉진용 조성물
WO2022045723A1 (ko) * 2020-08-24 2022-03-03 차의과학대학교 산학협력단 신경줄기세포의 도파민 신경세포로의 분화 촉진용 조성물
WO2023278382A1 (en) 2021-06-29 2023-01-05 Staffan Holmin Endoluminal delivery cannula

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KRABBE, C. ET AL.: "Enhanced dopaminergic differentiation of human neural stem cells by synergistic effect of Bel-XL and reduced oxygen tension", JOURNAL OF NEUROCHEMISTRY, vol. 110, 2009, pages 1908 - 1920, XP055098593 *
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Also Published As

Publication number Publication date
EP2581443A4 (en) 2013-10-23
CN102985535B (zh) 2014-11-05
CA2802661C (en) 2018-09-04
CA2802661A1 (en) 2011-12-22
AU2011265956B2 (en) 2015-12-03
EP2581443A2 (en) 2013-04-17
KR101106020B1 (ko) 2012-01-17
WO2011159050A2 (ko) 2011-12-22
US20130089926A1 (en) 2013-04-11
EP2581443B1 (en) 2015-01-07
JP5776101B2 (ja) 2015-09-09
JP2013528396A (ja) 2013-07-11
CN102985535A (zh) 2013-03-20
ES2532928T3 (es) 2015-04-06
AU2011265956A1 (en) 2013-01-10
US8921107B2 (en) 2014-12-30
KR20110136061A (ko) 2011-12-21

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