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WO2010059206A2 - Compositions de nucléotides modulaires et utilisations de celles-ci - Google Patents

Compositions de nucléotides modulaires et utilisations de celles-ci Download PDF

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Publication number
WO2010059206A2
WO2010059206A2 PCT/US2009/006177 US2009006177W WO2010059206A2 WO 2010059206 A2 WO2010059206 A2 WO 2010059206A2 US 2009006177 W US2009006177 W US 2009006177W WO 2010059206 A2 WO2010059206 A2 WO 2010059206A2
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WO
WIPO (PCT)
Prior art keywords
composition
group
linkage
covalent
coupled
Prior art date
Application number
PCT/US2009/006177
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English (en)
Other versions
WO2010059206A3 (fr
Inventor
Jonas Korlach
Jeffrey Wegener
Original Assignee
Pacific Biosciences Of California, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pacific Biosciences Of California, Inc. filed Critical Pacific Biosciences Of California, Inc.
Publication of WO2010059206A2 publication Critical patent/WO2010059206A2/fr
Publication of WO2010059206A3 publication Critical patent/WO2010059206A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites

Definitions

  • the functional group is coupled to the polyphosphate groups through a configurable linkage that allows in situ attachment of the functional group to the polyphosphate chain.
  • the linkage included within the compositions of the invention allows for a cassette approach to the selection and configuration of nucleotides in order to tailor such compositions for a desired application.
  • This cassette approach takes advantage of a facile linkage process which does not require the use of complex synthetic schemes to covalently couple functional groups to nucleotide compositions.
  • Use of such facile linkage processes permits one to rapidly choose and link a desired functional group to a nucleotide for use in a desired application. Further, one can configure sets of nucleotides according to a wide variety of application specific criteria.
  • a schematic illustration of the cassette-based linkage process is illustrated in Figure 1.
  • Figure 6A, B and C show detection of incorporation of: A568-SAv-Biotin-dT6P in conjunction with A648-dG6P ( Figure 6A), A568-dT6P in conjunction with A648-SAv-dG6P ( Figure 6B), and A568-SAv-Biotin-dT6P in conjunction with A648-SAv-dG6P ( Figure 6C).
  • Figure 6A Figure 6A
  • Figure 6B shows detection of incorporation of blocks of T and G analogs.

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

La présente invention concerne des compositions d’acide nucléique, des procédés de préparation et d’utilisation de telles compositions qui comprennent des groupes fonctionnels modulaires qui peuvent être configurés pour conférer une fonctionnalité souhaitée à différents types de nucléotide par l’intermédiaire d’un composant de liaison échangeable et de préférence non covalent. De telles compositions sont utiles dans différentes applications comprenant des analyses d’acide nucléique.
PCT/US2009/006177 2008-11-19 2009-11-18 Compositions de nucléotides modulaires et utilisations de celles-ci WO2010059206A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11620208P 2008-11-19 2008-11-19
US61/116,202 2008-11-19

Publications (2)

Publication Number Publication Date
WO2010059206A2 true WO2010059206A2 (fr) 2010-05-27
WO2010059206A3 WO2010059206A3 (fr) 2010-08-05

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2009/006177 WO2010059206A2 (fr) 2008-11-19 2009-11-18 Compositions de nucléotides modulaires et utilisations de celles-ci

Country Status (2)

Country Link
US (6) US8252910B2 (fr)
WO (1) WO2010059206A2 (fr)

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Also Published As

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US20180163267A1 (en) 2018-06-14
US20160244824A1 (en) 2016-08-25
US20130022968A1 (en) 2013-01-24
US10745750B2 (en) 2020-08-18
US20100152424A1 (en) 2010-06-17
US20190185926A1 (en) 2019-06-20
US20170159116A1 (en) 2017-06-08
US9879319B2 (en) 2018-01-30
WO2010059206A3 (fr) 2010-08-05
US8252910B2 (en) 2012-08-28
US8846881B2 (en) 2014-09-30
US9551031B2 (en) 2017-01-24
US10161002B2 (en) 2018-12-25

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