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WO2009063138A3 - Method for producing lipid - Google Patents

Method for producing lipid Download PDF

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Publication number
WO2009063138A3
WO2009063138A3 PCT/FI2008/050663 FI2008050663W WO2009063138A3 WO 2009063138 A3 WO2009063138 A3 WO 2009063138A3 FI 2008050663 W FI2008050663 W FI 2008050663W WO 2009063138 A3 WO2009063138 A3 WO 2009063138A3
Authority
WO
WIPO (PCT)
Prior art keywords
precipitate
lipid
source material
filtrate
thermo
Prior art date
Application number
PCT/FI2008/050663
Other languages
French (fr)
Other versions
WO2009063138A2 (en
Inventor
Ossi Pastinen
Simo Laakso
Sanna Hokkanen
Miia Mujunen
Original Assignee
Univ Helsinki Technology
Ossi Pastinen
Simo Laakso
Sanna Hokkanen
Miia Mujunen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from FI20075810A external-priority patent/FI123960B/en
Priority to NZ585022A priority Critical patent/NZ585022A/en
Priority to EP08848895A priority patent/EP2207886A2/en
Priority to JP2010533628A priority patent/JP2011502526A/en
Priority to AU2008322755A priority patent/AU2008322755B2/en
Priority to UAA201007433A priority patent/UA101637C2/en
Priority to CN2008801247413A priority patent/CN101910407A/en
Priority to CA2705323A priority patent/CA2705323A1/en
Application filed by Univ Helsinki Technology, Ossi Pastinen, Simo Laakso, Sanna Hokkanen, Miia Mujunen filed Critical Univ Helsinki Technology
Priority to AP2010005248A priority patent/AP2830A/en
Priority to MX2010005398A priority patent/MX2010005398A/en
Priority to EA201070434A priority patent/EA017664B1/en
Priority to BRPI0820369-5A priority patent/BRPI0820369A2/en
Publication of WO2009063138A2 publication Critical patent/WO2009063138A2/en
Publication of WO2009063138A3 publication Critical patent/WO2009063138A3/en
Priority to ZA2010/03066A priority patent/ZA201003066B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/649—Biodiesel, i.e. fatty acid alkyl esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6458—Glycerides by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P30/00—Technologies relating to oil refining and petrochemical industry
    • Y02P30/20—Technologies relating to oil refining and petrochemical industry using bio-feedstock

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The invention relates to a method for forming a lipid or a lipid mixture from an organic source material comprising cellulose, hemicellulose, starch, non-starch polysaccharide, any mixture thereof or degradation products thereof. According to the method, the source material is treated one or more times with water, an aqueous solution of acid or alkali, and the precipitate and the filtrate are separated. The precipitate obtained from the treatments mentioned above can be subjected to mechanical or thermo-mechanical grinding, or the precipitate can be treated with a strong acid, or the precipitate can be acidified and ground mechanically or thermo-mechanically. After the treatments, the precipitate and the filtrate are separated. A lipid-producing microorganism is contacted with the source material or the obtained filtrate(s) in a culture medium, whereby the microorganism cells begin to produce lipid, and the lipids are recovered.
PCT/FI2008/050663 2007-11-14 2008-11-14 Method for producing lipid WO2009063138A2 (en)

Priority Applications (12)

Application Number Priority Date Filing Date Title
BRPI0820369-5A BRPI0820369A2 (en) 2007-11-14 2008-11-14 Method for lipid production
CA2705323A CA2705323A1 (en) 2007-11-14 2008-11-14 Method for producing lipid
JP2010533628A JP2011502526A (en) 2007-11-14 2008-11-14 Lipid production method
AU2008322755A AU2008322755B2 (en) 2007-11-14 2008-11-14 Method for producing lipid
UAA201007433A UA101637C2 (en) 2007-11-14 2008-11-14 Method for lipid production
CN2008801247413A CN101910407A (en) 2007-11-14 2008-11-14 Method for producing lipid
AP2010005248A AP2830A (en) 2007-11-14 2008-11-14 Method for producing lipid
NZ585022A NZ585022A (en) 2007-11-14 2008-11-14 Method for producing lipid
EP08848895A EP2207886A2 (en) 2007-11-14 2008-11-14 Method for producing lipid
MX2010005398A MX2010005398A (en) 2007-11-14 2008-11-14 Method for producing lipid.
EA201070434A EA017664B1 (en) 2007-11-14 2008-11-14 Method for producing lipid
ZA2010/03066A ZA201003066B (en) 2007-11-14 2010-05-03 Method for producing lipid

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US305607P 2007-11-14 2007-11-14
FI20075810A FI123960B (en) 2007-11-14 2007-11-14 A process for producing lipid
FI20075810 2007-11-14
US61/003,056 2007-11-14

Publications (2)

Publication Number Publication Date
WO2009063138A2 WO2009063138A2 (en) 2009-05-22
WO2009063138A3 true WO2009063138A3 (en) 2009-08-13

Family

ID=42261768

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FI2008/050663 WO2009063138A2 (en) 2007-11-14 2008-11-14 Method for producing lipid

Country Status (8)

Country Link
EP (1) EP2207886A2 (en)
KR (1) KR20100091217A (en)
AP (1) AP2830A (en)
AU (1) AU2008322755B2 (en)
CA (1) CA2705323A1 (en)
MX (1) MX2010005398A (en)
WO (1) WO2009063138A2 (en)
ZA (1) ZA201003066B (en)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9499635B2 (en) 2006-10-13 2016-11-22 Sweetwater Energy, Inc. Integrated wood processing and sugar production
IT1392910B1 (en) * 2008-10-21 2012-04-02 Eni Spa PROCEDURE FOR THE PRODUCTION OF BIOMASS LIPIDS
WO2010068637A1 (en) 2008-12-09 2010-06-17 Jerry Wayne Horton Ensiling biomass and multiple phase apparatus for hydrolyzation of ensiled biomass
FI122957B (en) * 2009-06-24 2012-09-14 Neste Oil Oyj A process for producing fat
KR101039792B1 (en) 2009-10-28 2011-06-09 주식회사 창해에탄올 Pretreatment device for fabricating of bio fuel and bio chemical material, and pretreatment process and fabricating process of bio fuel and bio chemical material using the same
BR112012022339A2 (en) * 2010-03-11 2015-10-06 Bp Biofuels Uk Ltd biological oils or biofuels and isolated organism and method for producing the same
ES2685502T3 (en) * 2010-05-25 2018-10-09 Neste Oyj Process and microorganisms for lipid production
IT1403244B1 (en) * 2010-10-22 2013-10-17 Eni Spa PROCEDURE FOR THE PRODUCTION OF BIOMASS LIPIDS.
ES2748848T3 (en) * 2010-12-22 2020-03-18 Neste Oyj Enzyme production process
PT2468857E (en) 2010-12-22 2014-12-22 Neste Oil Oyj An integrated process system for lipid production and pulping
US8765430B2 (en) 2012-02-10 2014-07-01 Sweetwater Energy, Inc. Enhancing fermentation of starch- and sugar-based feedstocks
DK2836602T3 (en) * 2012-04-13 2019-01-28 Sweetwater Energy Inc Methods and systems for biomass suction
US8563277B1 (en) 2012-04-13 2013-10-22 Sweetwater Energy, Inc. Methods and systems for saccharification of biomass
ITMI20122249A1 (en) * 2012-12-28 2014-06-29 Eni Spa PROCEDURE FOR THE PRODUCTION OF BIOMASS LIPIDS
US9809867B2 (en) 2013-03-15 2017-11-07 Sweetwater Energy, Inc. Carbon purification of concentrated sugar streams derived from pretreated biomass
EP3134535B1 (en) 2014-04-23 2020-07-01 ENI S.p.A. Process for the production of lipids from biomass employing oleaginous microorganisms
DK3230463T3 (en) 2014-12-09 2022-08-22 Sweetwater Energy Inc FAST PRE-PROCESSING
EP3106520A1 (en) * 2015-06-17 2016-12-21 Institut National De La Recherche Agronomique Mutant yarrowia strain capable of degrading galactose
CN105969676B (en) * 2016-07-04 2020-07-28 江西师范大学 Method for regulating fermentation form of Trichosporon dermatomyces B3
EP3583223A4 (en) 2017-02-16 2020-12-23 Sweetwater Energy, Inc. High pressure zone formation for pretreatment
CN110387332A (en) * 2019-07-09 2019-10-29 天津大学 It is a kind of to be accumulated using artificial municipal wastewater culture chlorella and extract the research method of protein
WO2021133733A1 (en) 2019-12-22 2021-07-01 Sweetwater Energy, Inc. Methods of making specialized lignin and lignin products from biomass
JP2023514832A (en) * 2020-02-19 2023-04-11 ザ ガバメント オブ ザ ユナイテッド ステイツ オブ アメリカ，アズ リプレゼンテッド バイ ザ セクレタリー オブ ザ ネイビー biofilm bioreactor

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4368056A (en) * 1981-05-20 1983-01-11 Pierce Sammy M Diesel fuel by fermentation of wastes
US5221357A (en) * 1979-03-23 1993-06-22 Univ California Method of treating biomass material
WO2001032715A1 (en) * 1999-11-02 2001-05-10 Waste Energy Integrated Sytems, Llc Process for the production of organic products from lignocellulose containing biomass sources

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5221357A (en) * 1979-03-23 1993-06-22 Univ California Method of treating biomass material
US4368056A (en) * 1981-05-20 1983-01-11 Pierce Sammy M Diesel fuel by fermentation of wastes
WO2001032715A1 (en) * 1999-11-02 2001-05-10 Waste Energy Integrated Sytems, Llc Process for the production of organic products from lignocellulose containing biomass sources

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ANGERBAUER C ET AL: "Conversion of sewage sludge into lipids by Lipomyces starkeyi for biodiesel production", BIORESOURCE TECHNOLOGY, vol. 99, no. 8, 24 August 2007 (2007-08-24), pages 3051 - 3056, XP002532683, ISSN: 0960-8524 *
DAI CHUAN-CHAO ET AL: "Biodiesel generation from oleaginous yeast Rhodotorula glutinis with xylose assimilating capacity", AFRICAN JOURNAL OF BIOTECHNOLOGY, vol. 6, no. 18, September 2007 (2007-09-01), pages 2130 - 2134, XP002532682, ISSN: 1684-5315 *
FALL R ET AL: "BIO CONVERSION OF XYLAN TO TRI GLYCERIDES BY OIL RICH YEASTS", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 47, no. 5, 1984, pages 1130 - 1134, XP002532684, ISSN: 0099-2240 *
JACOB Z ET AL: "STUDIES ON PHYSICOCHEMICAL CHARACTERISTICS AND FATTY ACID COMPOSITION OF LIPIDS PRODUCED BY A STRAIN OF RHODOTORULA-GRACILIS CFR-1", JOURNAL OF THE AMERICAN OIL CHEMISTS' SOCIETY, vol. 67, no. 10, 1990, pages 642 - 645, XP002532685, ISSN: 0003-021X *

Also Published As

Publication number Publication date
WO2009063138A2 (en) 2009-05-22
ZA201003066B (en) 2011-06-29
AP2830A (en) 2014-01-31
MX2010005398A (en) 2010-09-10
AU2008322755B2 (en) 2013-11-14
AU2008322755A1 (en) 2009-05-22
CA2705323A1 (en) 2009-05-22
KR20100091217A (en) 2010-08-18
AP2010005248A0 (en) 2010-06-30
EP2207886A2 (en) 2010-07-21

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