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WO2008005248A3 - Real-time pcr in micro-channels - Google Patents

Real-time pcr in micro-channels Download PDF

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Publication number
WO2008005248A3
WO2008005248A3 PCT/US2007/014863 US2007014863W WO2008005248A3 WO 2008005248 A3 WO2008005248 A3 WO 2008005248A3 US 2007014863 W US2007014863 W US 2007014863W WO 2008005248 A3 WO2008005248 A3 WO 2008005248A3
Authority
WO
WIPO (PCT)
Prior art keywords
real
micro
channels
time pcr
methods
Prior art date
Application number
PCT/US2007/014863
Other languages
French (fr)
Other versions
WO2008005248A2 (en
Inventor
Kenton C Hasson
Gregory A Dale
Hiroshi Inoue
Original Assignee
Canon Us Life Sciences Inc
Kenton C Hasson
Gregory A Dale
Hiroshi Inoue
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/505,358 external-priority patent/US7629124B2/en
Application filed by Canon Us Life Sciences Inc, Kenton C Hasson, Gregory A Dale, Hiroshi Inoue filed Critical Canon Us Life Sciences Inc
Priority to EP07796482.3A priority Critical patent/EP2046988A4/en
Priority to JP2009518241A priority patent/JP2009542210A/en
Publication of WO2008005248A2 publication Critical patent/WO2008005248A2/en
Publication of WO2008005248A3 publication Critical patent/WO2008005248A3/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/143—Quality control, feedback systems
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1838—Means for temperature control using fluid heat transfer medium
    • B01L2300/1844—Means for temperature control using fluid heat transfer medium using fans
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1838—Means for temperature control using fluid heat transfer medium
    • B01L2300/185—Means for temperature control using fluid heat transfer medium using a liquid as fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention relates to methods for amplifying nucleic acids in micro-channels. More specifically, the present invention relates to methods for performing a real-time polymerase chain reaction (PCR) in a continuous-flow microfluidic system and to methods for monitoring real-time PCR in such systems.
PCT/US2007/014863 2006-06-30 2007-06-27 Real-time pcr in micro-channels WO2008005248A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP07796482.3A EP2046988A4 (en) 2006-06-30 2007-06-27 Real-time pcr in micro-channels
JP2009518241A JP2009542210A (en) 2006-06-30 2007-06-27 Real-time PCR in microchannels

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US80644006P 2006-06-30 2006-06-30
US60/806,440 2006-06-30
US11/505,358 2006-08-17
US11/505,358 US7629124B2 (en) 2006-06-30 2006-08-17 Real-time PCR in micro-channels

Publications (2)

Publication Number Publication Date
WO2008005248A2 WO2008005248A2 (en) 2008-01-10
WO2008005248A3 true WO2008005248A3 (en) 2008-07-31

Family

ID=38877102

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/014863 WO2008005248A2 (en) 2006-06-30 2007-06-27 Real-time pcr in micro-channels

Country Status (1)

Country Link
WO (1) WO2008005248A2 (en)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5303983B2 (en) * 2008-03-26 2013-10-02 株式会社島津製作所 Reaction processing method and reaction processing apparatus
JP2009268432A (en) * 2008-05-09 2009-11-19 Canon Inc Method for measuring target nucleic acid
JP2010022315A (en) * 2008-07-23 2010-02-04 Sony Corp Nucleic acid quantity detection device and nucleic acid quantity detection method
WO2010118430A1 (en) * 2009-04-10 2010-10-14 Canon U.S. Life Sciences, Inc. A method of delivering pcr solution to microfluidic pcr chamber
EP2437887B1 (en) 2009-06-04 2016-05-11 Lockheed Martin Corporation Multiple-sample microfluidic chip for dna analysis
CA3016967C (en) * 2010-04-16 2021-08-31 Opko Diagnostics, Llc Systems and devices for analysis of samples
US9861985B2 (en) 2010-08-31 2018-01-09 Canon U.S. Life Sciences, Inc. Slug control during thermal cycling
CA2814720C (en) 2010-10-15 2016-12-13 Lockheed Martin Corporation Micro fluidic optic design
CN103748453A (en) * 2011-04-08 2014-04-23 斯多克斯生物有限公司 End-point optical system and method of use
CN102220226B (en) * 2011-05-23 2013-07-24 北京工业大学 Two-path temperature control polymerase chain reactor and real-time detection device
EP3231861B1 (en) 2012-02-03 2020-01-08 Axxin Pty Ltd Nucleic acid amplification with mathematical calculation of the assay result
CN104271767B (en) * 2012-02-03 2017-08-25 加州理工学院 The coding and decoding of signal in multichannel biochemical measurement
US9322054B2 (en) 2012-02-22 2016-04-26 Lockheed Martin Corporation Microfluidic cartridge
EP2864503B1 (en) 2012-06-26 2019-10-09 Axxin Pty Ltd Nucleic acid amplification and detection kit
EP2878374B1 (en) 2013-11-29 2021-05-12 IMEC vzw Method for performing digital PCR
AU2015346009B2 (en) 2014-11-14 2020-07-23 Axxin Pty Ltd Biological sample collection and storage assembly
GB2548764B (en) 2015-01-27 2020-10-21 Hitachi High Tech Corp Multicolor fluorescence analysis device
EP3325156A4 (en) 2015-07-17 2019-01-16 Axxin Pty Ltd Diagnostic test assembly, apparatus, method
BR112020006004A2 (en) 2017-09-27 2020-10-06 Axxin Pty Ltd diagnostic test system, assembly and method
CN112925006A (en) * 2019-12-05 2021-06-08 核工业西南物理研究院 Fusion neutron real-time energy spectrum resolution method for magnetic confinement device

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050202489A1 (en) * 2004-03-12 2005-09-15 Cho Yoon-Kyoung Method and apparatus for amplifying nucleic acids

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050202489A1 (en) * 2004-03-12 2005-09-15 Cho Yoon-Kyoung Method and apparatus for amplifying nucleic acids

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
PARK ET AL.: "Cylindrical compact thermal-cycling device for continous-flow polymerase chain reaction", ANAL. CHEM., vol. 75, no. 21, 1 November 2003 (2003-11-01), pages 6029 - 6033, XP001047342, DOI: doi:10.1021/ac0346959 *
PARK N. ET AL.: "Cylindrical compact thermal-cycling device for continuous-flow polymerase chain reaction", ANAL. CHEM., vol. 75, no. 21, 1 November 2003 (2003-11-01), pages 6029 - 6033, XP001047342 *
See also references of EP2046988A4 *

Also Published As

Publication number Publication date
WO2008005248A2 (en) 2008-01-10

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