Nothing Special   »   [go: up one dir, main page]

WO2008086996A1 - Dispositif d'éclairage à éléments optiques non linéaires pour produire une lumière laser dans un large domaine spectral avec une densité de puissance spectrale homogène - Google Patents

Dispositif d'éclairage à éléments optiques non linéaires pour produire une lumière laser dans un large domaine spectral avec une densité de puissance spectrale homogène Download PDF

Info

Publication number
WO2008086996A1
WO2008086996A1 PCT/EP2008/000232 EP2008000232W WO2008086996A1 WO 2008086996 A1 WO2008086996 A1 WO 2008086996A1 EP 2008000232 W EP2008000232 W EP 2008000232W WO 2008086996 A1 WO2008086996 A1 WO 2008086996A1
Authority
WO
WIPO (PCT)
Prior art keywords
laser radiation
module
nonlinear
emitted
laser
Prior art date
Application number
PCT/EP2008/000232
Other languages
German (de)
English (en)
Other versions
WO2008086996A8 (fr
Inventor
Peter Westphal
Original Assignee
Carl Zeiss Microimaging Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Carl Zeiss Microimaging Gmbh filed Critical Carl Zeiss Microimaging Gmbh
Publication of WO2008086996A1 publication Critical patent/WO2008086996A1/fr
Publication of WO2008086996A8 publication Critical patent/WO2008086996A8/fr

Links

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G—PHYSICS
    • G02—OPTICS
    • G02F—OPTICAL DEVICES OR ARRANGEMENTS FOR THE CONTROL OF LIGHT BY MODIFICATION OF THE OPTICAL PROPERTIES OF THE MEDIA OF THE ELEMENTS INVOLVED THEREIN; NON-LINEAR OPTICS; FREQUENCY-CHANGING OF LIGHT; OPTICAL LOGIC ELEMENTS; OPTICAL ANALOGUE/DIGITAL CONVERTERS
    • G02F1/00—Devices or arrangements for the control of the intensity, colour, phase, polarisation or direction of light arriving from an independent light source, e.g. switching, gating or modulating; Non-linear optics
    • G02F1/35—Non-linear optics
    • G02F1/3528—Non-linear optics for producing a supercontinuum

Definitions

  • the present invention relates to a lighting device that is particularly suitable for a microscope. Furthermore, the invention relates to a corresponding illumination method.
  • the illumination device is intended to be able to deliver any desired radiation in the spectral range from UV (about 300 nm) to IR (about 2000 nm), the range from 350 to 1000 nm being preferred.
  • US Pat. No. 6,154,310 describes a laser system in which ultrashort pulses are coupled into an optical splitter. In each of the branches of the splitter, wavelength conversion occurs via harmonic or parametric generation. Subsequently, the branches are united again into a beam.
  • the disadvantage here is that after conversion only a few discrete wavelengths are available.
  • US Pat. No. 6,888,674 B1 describes a scanning microscope which contains a primary laser and a single optical component which spectrally widens the primary laser light of the primary laser in such a way that it contains a substantial portion of the entire visible spectrum behind the optical component.
  • the visible spectrum which is generated by the individual optical component, has a disadvantageous course for microscopy, since little light power is delivered in the green-yellow spectral range.
  • WO 2005/119328 A1 describes a light source for a scanning microscope, in which a photonic crystal fiber (PCF) is used to generate continuum.
  • PCF photonic crystal fiber
  • Fig. 2 of WO 2005/119328 A1 a measured power spectrum is shown, wherein the PCF length was chosen so that the total continuum power in the visible spectral range was maximum.
  • This spectrum shows extreme break-ins at different wavelength ranges, in particular around 505 nm and around 545 nm.
  • the PCF length it is also possible to change the length of the laser pulses in order to vary the spectrum. As a result, the gaps are usually only shifted spectrally.
  • DE 101 39 754 B4 describes a device in which both the primary laser light and the frequency-converted by a component laser light is used for microscopic purposes. Apart from the additional use of the primary laser light, this illumination device does not differ significantly from the illumination device of US Pat. No. 6,888,674 B1 and thus also has its disadvantages.
  • EP 1 558 967 B1 describes a device for generating pulsed light in the visible spectral range, which is similar to the illumination device of US Pat. No. 6,888,674 B1.
  • additional optical means are provided to influence the pulse duration.
  • These optical means may also include movable components, thereby significantly increasing the complexity and manufacturing cost of the device.
  • the object is achieved by a device for illuminating an object, according to claim 1.
  • the spectral power density in the entire visible spectral range is relatively constant. In particular, extreme dips in the spectrum are avoided.
  • control loop which measures intensity fluctuations of the laser radiation coming from the module and controls a controllable component of the device depending on the measured intensity fluctuations such that the intensity fluctuations are at least partially compensated, a very constant spectral power density in the entire visible spectral range can be achieved.
  • the device for illuminating may be part of a microscope in particular, so that a microscope is provided with a lighting device that can deliver laser radiation with nearly constant spectral power at least in the green-yellow spectral range and preferably in the entire visible spectral range.
  • the microscope can be a point or line scanning microscope which operates confocally or partially confocal. Furthermore, it may also be a microscope that operates on the principle SPIM (Selective Plane Illumination Microscopy). The microscope may also be an optically operating cytometer or an optically operating Biochiop reader.
  • SPIM Selective Plane Illumination Microscopy
  • the illumination device can be used both for detection and manipulation of microscopic objects.
  • the microscope can be used in particular in the field of fluorescence microscopy. It is particularly suitable for simultaneous or quasi-simultaneous (i.e., with fast wavelength switching) excitation of multiple fluorescent dyes. Because the illumination device of the microscope provides visible light and infrared radiation, it is suitable for both single-photon and multi-photon excitation.
  • OCT Optical Coherence Topography
  • CARS Coherent Anti-Stokes Raman Spectroscopy
  • the wide spectrum of the illumination device can be used advantageously, since in this way a low temporal coherence and thus a high spatial resolution is possible.
  • a CARS microscope can be used to advantage that the at least two different, for the CARS necessary wavelengths can be varied continuously with the lighting device.
  • the microscope can also be embodied as a material microscope, as a cytometer, as a laser-based biochiop reader or as a fundus camera.
  • the laser can emit laser radiation in the infrared spectrum and the spectrum of the laser radiation emitted by the module can extend into the visible region.
  • the spectrum of the laser radiation emitted by the first nonlinear element to lie exclusively in the infrared spectral range.
  • the non-linear optical elements can be designed so that complement the spectral broadening of the individual nonlinear optical elements as complementary as possible, so that a total of a very wide range can be achieved.
  • the spectral ranges of the individual nonlinear optical elements may overlap.
  • At least one of the optical elements may be formed as a tapered optical fiber.
  • the dispersion zero point of the group velocity of the optical fiber is preferably chosen so that it lies below the wavelength of the coupled-in laser radiation. This can be achieved, for example, by varying the length of the tapered portion of the fiber.
  • At least one of the non-linear optical elements may be formed as a photonic crystal fiber, which are often also called microstructured glass fibers.
  • At least one of the elements may also be formed as a nonlinear crystal.
  • a spectral filter unit can be arranged between the module and the illumination optics.
  • This filter unit may be controllable, e.g. advantageous in the use of the illumination optics in a CARS microscope.
  • the wavelength or the wavelengths can be filtered out of the spectrum of the laser radiation emitted by the module, which are required for the respective application.
  • the controllable component can be designed as a controllable intensity modulator, which is arranged between the module and the illumination optics. , ,
  • the component may be e.g. also the laser itself and / or a component in the illumination optics.
  • a device for illuminating an object having an illumination optical system, a module arranged upstream of the illumination optical unit, which has at least two non-linear optical elements arranged one behind the other, and a window
  • Module upstream laser that emits laser radiation to the module, which passes in sequence through the elements arranged one behind the other and is delivered to the illumination optics, which can illuminate the object with the laser radiation coming from the module, each non-linear element causes due to its non-linear effect, that the spectral
  • Bandwidth of the laser radiation emitted by the nonlinear element is greater than the spectral bandwidth of the laser radiation coupled into the nonlinear element.
  • the object is further achieved by a method according to claim 10.
  • the primary laser radiation may be in the infrared spectrum and the spectrum of the laser radiation emitted by the last element may extend into the visible region.
  • the spectrum of the laser radiation emitted by the first non-linear element, to which primary laser radiation is incident, can lie exclusively in the infrared spectral range.
  • At least one of the optical elements may be formed as a tapered optical fiber. It is also possible to form at least one of the non-linear optical elements as a photonic crystal fiber or as a nonlinear crystal.
  • the laser radiation emitted by the last element can be spectrally filtered and the object can be illuminated with the spectrally filtered laser radiation.
  • the laser radiation coming from the last element can be guided through a controllable intensity modulator, which is controlled accordingly and emits the laser radiation with reduced intensity fluctuations for illumination of the object.
  • the illumination of the object can be carried out in particular for a microscopy method, so that a microscopy method is provided which has an illumination which has an approximately constant spectral power density in the visible spectral range.
  • a method for illuminating an object in which a primary laser radiation passes through at least two nonlinear optical elements arranged one behind the other and the laser radiation emitted by the last optical element is used to illuminate the optical object, each nonlinear element due to its nonlinear effect causes the spectral bandwidth of the laser radiation emitted by the non-linear element is greater than the spectral bandwidth of the laser radiation coupled into the non-linear element.
  • Fig. 1 is a schematic representation of a device for illuminating an object for explaining an embodiment of the invention
  • FIGS. 2a-2e each show the spectral power of the laser radiation emitted by the individual elements
  • FIG 3 shows an embodiment of the lighting device according to the invention
  • Fig. 4 shows a further embodiment of the lighting device according to the invention.
  • Fig. 5 shows a further embodiment of the lighting device according to the invention.
  • the device 1 for illuminating an object comprises a laser 2, a module 3 with three nonlinear optical elements 4, 5, 6, a spectral filter 7 and an illumination optical system 8, for example, be part of a microscope can.
  • the laser 2 emits pulsed laser radiation having a wavelength of 1064 nm and a pulse length of 5 fs at a pulse rate of 100 MHz and a mean optical power of 25 watts.
  • the spectral power (y-axis) of the laser radiation of the laser 2 as a function of wavelength in nm (x-axis) is plotted.
  • the laser radiation of the laser 2 strikes the first non-linear optical element 4, which may be formed for example as a highly tapered glass fiber.
  • the first nonlinear optical element 4 At the output of the first nonlinear optical element 4, a broader spectrum of the laser radiation is present in comparison to the spectrum of the laser radiation L1 impinging on the first nonlinear optical element 4.
  • the spectral power of the laser radiation L2 emitted by the first non-linear optical element is shown schematically in FIG. 2b, wherein the wavelength of the coupled-in laser radiation L1 is denoted by L1.
  • the scale of the y-axis may differ in FIGS. 2a-2e, since FIGS. 2a-2e are only intended to illustrate the qualitative course of the spectral power.
  • the first non-linear optical element 4 is designed so that a significant light output is present only for wavelengths greater than 800 nm and thus exclusively in the infrared spectral range.
  • the laser radiation L2 then impinges on the second non-linear optical element 5, which due to non-linear optical effects leads to a broadening of the spectral bandwidth and emits laser radiation L3.
  • the spectral power of the laser beams L3 is shown schematically in FIG. 2c.
  • the laser radiation L3 passes through the third non-linear optical element 6, which leads to a further spectral broadening of the bandwidth.
  • the emitted laser radiation L4 has an approximately equal spectral power density in the visible range, as can be seen from FIG. 2d.
  • the laser radiation L4 strikes a spectral filter 7, which filters out the desired wavelengths from the spectrum of the laser radiation L4 and supplies them to the illumination optics 8 as laser radiation L5.
  • the spectral power of the laser radiation L5 is shown in FIG. 2e.
  • the spectral filter 7 may also be omitted, e.g. if a broadband laser radiation L4 is desired or a spectral filtering in the illumination optical system 8 takes place.
  • the illumination device 1 thus comprises a module with three non-linear optical elements, which lead to a spectral broadening of the laser radiation L1 of the laser 2, , ,
  • the broadening due to the non-linear optical elements 4 to 6 connected in series causes the spectral power density in the visible spectral range to be approximately the same or at least to have no pronounced dips in the visible wavelength range.
  • FIG. 3 shows a development of the lighting device 1 according to the invention of FIG. 1, wherein identical elements are designated by the same reference numerals and reference is made to the above explanations for their description.
  • a fast photodiode 9 (which can be a PIN or an avalanche photodiode, for example, a PIN or an avalanche photodiode) is additionally arranged between the spectral filter 7 and the illumination optics 8 in conjunction with a partial beam extraction for measuring the intensity of the laser radiation L 4, wherein only a small portion of the radiation L5 is coupled out for the photodiode 9.
  • the temporal intensity fluctuation measured with the photodiode 9 is supplied to a control system 10 (arrow P1) which influences the laser 2 (as indicated by the arrow P2) to counteract the intensity fluctuation.
  • the fluctuation is measured in terms of time and extrapolated from the intensity changes of the future intensity course.
  • the laser 2 is then influenced (as indicated by the arrow P2) so that the intensity fluctuations of the extrapolated intensity curve are compensated.
  • the control system 10 can influence not only the laser 2 but, for example, also the spectral filter 7 (arrow P3). Furthermore, it is possible for the control system to influence an optionally provided intensity modulator 11 (shown in dashed lines in FIG. 3) (arrow P3), which is arranged between the photodiode 9 and the illumination optical unit 8.
  • the intensity modulator 11 can be designed, for example, as an acousto-optic modulator (AOM) or as an electro-optical modulator (EOM).
  • control system 10 may drive a controllable component (not shown) of the illumination optics 8, as shown schematically by the arrow P5.
  • the controllable component of the illumination optical unit 8 may be, for example, an AOM, an EOM, a tilting mirror matrix, an LCD element, a mechanically changeable diaphragm or an attenuator.
  • the control system 10 can use one of the control circuits P2-P5, several of the control circuits P2-P5 or even all of the control circuits P2-P5. If the control loop P4 is not used, the intensity modulator 11 is omitted.
  • FIG. 4 a further embodiment of the lighting device 1 is shown schematically, wherein the same elements as in the embodiment of Fig. 1 are designated by the same reference numerals and reference is made to the description thereof to the above statements.
  • the embodiment of FIG. 4 may comprise a control system 10 in the same way as in FIG.
  • an optical diode 12 is disposed between the laser 2 and the first non-linear optical element 4.
  • the optical diode 12 serves to avoid interference of the laser 2 by back reflections from the further beam path (to the right of the diode 12 in FIG. 4).
  • the first non-linear optical element 4 is designed here as a tapered fiber whose dispersion zero point of the group velocity is below the wavelength of the laser radiation L1 of 1064 nm. This ensures that the laser radiation L1 lies in the abnormal dispersion region of the tapered fiber 4, which is a prerequisite for effective continuum generation.
  • the tapered portion of the first nonlinear optical element 4 has a length of 50-200 mm and can be used as a design parameter.
  • the length of the tapered portion can be selected so that the spectral range of the laser radiation L2 emitted from the first nonlinear optical element 4 extends from about 800 to 1300 nm.
  • the diameter of the tapered area is about 2.5 microns.
  • the fibers 4 and 5 can also be optically connected by means of direct splicing (fusing or gluing).
  • the PCF 5 is designed so that its dispersion zero point is below 800 nm, so that it is ensured here that the wavelengths of the spectral range of the laser radiation L2 are in the abnormal dispersion range of the PCF 5.
  • the length of the PCF 5 is a design parameter. The length is chosen so that the visible continuum of the wavelength L3 behind the PCF 5 has the highest possible optical power and / or spectrally possible is homogeneous.
  • the core diameter and the diameter of the PCF 5 air holes are each about 2 ⁇ m.
  • the length of the PCF 5 is in the range of 0.5 - 10 m.
  • the module 3 thus exactly two nonlinear optical elements 4, 5, which are preferably selected so that the continuums generated by them are as complementary as possible, so that in the sum of a substantially homogeneous spectrum.
  • the module 3 may have more than two nonlinear optical elements. This can be used, for example, to further improve the spectral homogeneity.
  • FIG. 5 shows a modification of the lighting device 1 of FIG. 4.
  • the embodiment of FIG. 5 can also have a control system 10 in the same way as in FIG. 3.
  • the laser 2 emits laser radiation having a wavelength of 1064 nm with a pulse length of 300 fs and a pulse rate of 50 MHz at an average optical power of 5 watts.
  • the tapered portion of the fiber 4 in Fig. 5 has a diameter of 3 microns and thus a slightly larger Diameter as the tapered portion of the fiber 4 of the lighting device 1 of Fig. 4 on.
  • the second non-linear optical element 5 is also in the embodiment of Fig. 5 formed as a tapered fiber (and not as PCF), with their dispersion zero point is below 800 nm.
  • the length of the tapered portion of the fiber 5 is again a design parameter and is in the range of 50-200 mm; the diameter of the tapered section is about 2 microns.
  • the described embodiments can be trained or further developed as a microscope.

Landscapes

  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Optical Modulation, Optical Deflection, Nonlinear Optics, Optical Demodulation, Optical Logic Elements (AREA)
  • Microscoopes, Condenser (AREA)
  • Lasers (AREA)

Abstract

L'invention concerne un dispositif pour éclairer un objet, comprenant : une optique d'éclairage (8) ; un module (3) précédant l'optique d'éclairage (8) et présentant au moins deux éléments optiques non linéaires (4 ; 5 ; 6) qui sont disposés en succession ; et un laser (2) précédant le module (3) et délivrant au module (3) un rayonnement laser (L1) qui traverse dans l'ordre les éléments (4-6) disposés en succession et est délivré à l'optique d'éclairage (8), qui peut éclairer l'objet avec le rayonnement laser (L4) provenant du module (3). Chaque élément non linéaire (4 ; 5 ; 6), du fait de son action non linéaire, a pour effet que la largeur de bande spectrale du rayonnement laser (L2 ; L3 ; L4) délivré par l'élément non linéaire (4 ; 5 ; 6) est supérieure à la largeur de bande spectrale du rayonnement laser (L1 ; L2 ; L3) injecté dans l'élément non linéaire (4 ; 5 ; 6). Le dispositif présente un circuit de régulation qui mesure les fluctuations d'intensité du rayonnement laser (L4) provenant du module (3) et qui, en fonction des fluctuations d'intensité mesurées, commande un élément asservissable du dispositif de telle sorte que les fluctuations d'intensité sont au moins partiellement compensées.
PCT/EP2008/000232 2007-01-16 2008-01-14 Dispositif d'éclairage à éléments optiques non linéaires pour produire une lumière laser dans un large domaine spectral avec une densité de puissance spectrale homogène WO2008086996A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102007002203A DE102007002203A1 (de) 2007-01-16 2007-01-16 Beleuchtungsvorrichtung und Beleuchtungsverfahren
DE102007002203.6 2007-01-16

Publications (2)

Publication Number Publication Date
WO2008086996A1 true WO2008086996A1 (fr) 2008-07-24
WO2008086996A8 WO2008086996A8 (fr) 2010-10-14

Family

ID=39149233

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2008/000232 WO2008086996A1 (fr) 2007-01-16 2008-01-14 Dispositif d'éclairage à éléments optiques non linéaires pour produire une lumière laser dans un large domaine spectral avec une densité de puissance spectrale homogène

Country Status (2)

Country Link
DE (1) DE102007002203A1 (fr)
WO (1) WO2008086996A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102008018476B4 (de) 2008-04-11 2022-12-15 Carl Zeiss Microscopy Gmbh Mikroskopievorrichtung
DE102009024941A1 (de) * 2009-06-09 2010-12-23 Carl Zeiss Surgical Gmbh Beleuchtungsvorrichtung und medizinisch-optisches Beobachtungsgerät
DE102012218624B4 (de) * 2012-09-07 2020-07-09 Leica Microsystems Cms Gmbh Konfokales Laser-Raster-Mikroskop mit einer gepulst angesteuerten Laserlichtquelle

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0886174A2 (fr) * 1997-06-18 1998-12-23 Nippon Telegraph And Telephone Corporation Source d'impulsions de lumière blanche
US6888674B1 (en) * 2000-06-17 2005-05-03 Leica Microsystems Heidelberg Gmbh Arrangement for examining microscopic preparations with a scanning microscope, and illumination device for a scanning microscope
WO2005119328A1 (fr) * 2004-06-03 2005-12-15 University Of Strathclyde Source lumineuse amelioree pour microscope a balayage
DE102004032463A1 (de) * 2004-06-30 2006-02-09 Jenoptik Laser, Optik, Systeme Gmbh Verfahren und optische Anordnung zur Erzeugung eines Breitbandspektrums

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6154310A (en) 1997-11-21 2000-11-28 Imra America, Inc. Ultrashort-pulse source with controllable multiple-wavelength output
DE10139754B4 (de) 2001-08-13 2004-07-08 Leica Microsystems Heidelberg Gmbh Beleuchtungsverfahren für ein Scanmikroskop und Scanmikroskop
DE102004009068B4 (de) 2003-02-25 2007-05-24 Toptica Photonics Ag Faseroptische Verstärkung von Lichtimpulsen
DE10340964A1 (de) * 2003-09-05 2005-03-31 Leica Microsystems Heidelberg Gmbh Lichtquelle mit einem mikrostrukturierten optischen Element

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0886174A2 (fr) * 1997-06-18 1998-12-23 Nippon Telegraph And Telephone Corporation Source d'impulsions de lumière blanche
US6888674B1 (en) * 2000-06-17 2005-05-03 Leica Microsystems Heidelberg Gmbh Arrangement for examining microscopic preparations with a scanning microscope, and illumination device for a scanning microscope
WO2005119328A1 (fr) * 2004-06-03 2005-12-15 University Of Strathclyde Source lumineuse amelioree pour microscope a balayage
DE102004032463A1 (de) * 2004-06-30 2006-02-09 Jenoptik Laser, Optik, Systeme Gmbh Verfahren und optische Anordnung zur Erzeugung eines Breitbandspektrums

Also Published As

Publication number Publication date
DE102007002203A1 (de) 2008-07-17
WO2008086996A8 (fr) 2010-10-14

Similar Documents

Publication Publication Date Title
DE19622359B4 (de) Vorrichtung zur Einkopplung der Strahlung von Kurzpulslasern in einem mikroskopischen Strahlengang
EP1184701B1 (fr) Dispositif d'éclairage
EP1164406B1 (fr) Méthode et appareil pour illuminer un objet
EP1714187B1 (fr) Microscope comprenant une source de lumiere comprenant plusieurs elements optiques microstructures
DE10243449B4 (de) CARS-Mikroskop und Verfahren zur CARS-Mikroskopie
DE102011106916B4 (de) Konfokales Auflicht-Rastermikroskop, Verfahren und Programm zum Betrieb eines solchen Mikroskops
DE10115589B4 (de) Konfokales Scanmikroskop
EP1186882B1 (fr) Procédé et dispositif pour la détection de lumière fluorescente dans la microscopie confocale à balayage
DE10235914A1 (de) Lichtquelle zur Beleuchtung mikroskopischer Objekte und Scanmikroskopsystem
DE20122791U1 (de) Scanmikroskop
EP1164400B1 (fr) Dispositif d'inspection des preparations microscopiques avec un microscope à balayage et dispositif d'illumination pour un microscope à balayage
DE10139754B4 (de) Beleuchtungsverfahren für ein Scanmikroskop und Scanmikroskop
DE102004032463B4 (de) Verfahren und optische Anordnung zur Erzeugung eines Breitbandspektrums mittels modengekoppelter Picosekunden-Laserimpulse
WO2008086996A1 (fr) Dispositif d'éclairage à éléments optiques non linéaires pour produire une lumière laser dans un large domaine spectral avec une densité de puissance spectrale homogène
DE102007039498B4 (de) Verfahren und Vorrichtung zur Beleuchtung und/oder Bestrahlung eines Objekts oder einer Probe
DE29609850U1 (de) Vorrichtung zur Einkopplung der Strahlung von Kurzpulslasern in einem mikroskopischen Strahlengang
DE19744302B4 (de) Vorrichtung zur Einkopplung der Strahlung von Kurzpulslasern in einem mikroskopischen Strahlengang
DE10313987B4 (de) Vorrichtung und Verfahren zur Beleuchtung eines Objekts
WO2003096116A1 (fr) Oscillateur et amplificateur parametriques otiques
DE102007009659B4 (de) Verwendung eines diodengepumpten Festkörperlasers und Laser-Scanning-Mikroskop mit einem UV-Beleuchtungsstrahlengang
DE102022206605A1 (de) Beleuchtungsvorrichtung und Mikroskopieverfahren zum Erzeugen eines zusammengesetzten Bildes einer Probe
DE20122785U1 (de) Vorrichtung zur Beleuchtung eines Objekts
DE20022256U1 (de) Anordnung zum Untersuchen mikroskopischer Präparate mit einem Scanmikroskop und Beleuchtungseinrichtung für ein Scanmikroskop

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 08707036

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 08707036

Country of ref document: EP

Kind code of ref document: A1