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WO2007123684A3 - Methods and devices for thawing and/or imaging frozen biological samples - Google Patents

Methods and devices for thawing and/or imaging frozen biological samples Download PDF

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Publication number
WO2007123684A3
WO2007123684A3 PCT/US2007/007915 US2007007915W WO2007123684A3 WO 2007123684 A3 WO2007123684 A3 WO 2007123684A3 US 2007007915 W US2007007915 W US 2007007915W WO 2007123684 A3 WO2007123684 A3 WO 2007123684A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
thawing
devices
imaging
biological samples
Prior art date
Application number
PCT/US2007/007915
Other languages
French (fr)
Other versions
WO2007123684A2 (en
Inventor
Mark A Voelker
Original Assignee
Biotime Inc
Mark A Voelker
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/601,442 external-priority patent/US20070231787A1/en
Application filed by Biotime Inc, Mark A Voelker filed Critical Biotime Inc
Publication of WO2007123684A2 publication Critical patent/WO2007123684A2/en
Publication of WO2007123684A3 publication Critical patent/WO2007123684A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/42—Low-temperature sample treatment, e.g. cryofixation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0278—Physical preservation processes
    • A01N1/0284—Temperature processes, i.e. using a designated change in temperature over time
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0278—Physical preservation processes
    • A01N1/0289—Pressure processes, i.e. using a designated change in pressure over time
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31—Apparatus therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00346—Heating or cooling arrangements

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

Methods of freezing, thawing and imaging a biological sample, one or more times, in a manner sufficient to freeze, thaw, and/or image the biological sample, while maintaining the structural integrity and/or viability of the sample are provided. Aspects of embodiments of the methods include freezing, thawing and/or imaging a biological sample under pressure in a manner sufficient to maintain the structural integrity and/or viability of the sample. Also provided are devices and systems for use in practicing the methods.
PCT/US2007/007915 2006-04-04 2007-03-30 Methods and devices for thawing and/or imaging frozen biological samples WO2007123684A2 (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US78954106P 2006-04-04 2006-04-04
US60/789,541 2006-04-04
US78954106A 2006-11-17 2006-11-17
US11/789,541 2006-11-17
US11/601,442 US20070231787A1 (en) 2006-04-04 2006-11-17 Methods and devices for imaging and manipulating biological samples
US11/601,442 2006-11-17

Publications (2)

Publication Number Publication Date
WO2007123684A2 WO2007123684A2 (en) 2007-11-01
WO2007123684A3 true WO2007123684A3 (en) 2008-12-11

Family

ID=38625465

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/007915 WO2007123684A2 (en) 2006-04-04 2007-03-30 Methods and devices for thawing and/or imaging frozen biological samples

Country Status (1)

Country Link
WO (1) WO2007123684A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011022369A2 (en) * 2009-08-18 2011-02-24 Lifecell Corporation Method for processing tissues
CN106404614A (en) * 2016-08-25 2017-02-15 哈尔滨商业大学 Ice crystal observation method of ice structure protein
CN107402213B (en) * 2017-08-29 2023-09-22 苏州欧谱曼迪科技有限公司 Probe, probe device and microscope system
CN118443669B (en) * 2024-07-08 2024-09-13 深圳市中医院 Biological tissue low temperature section imaging device

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5257128A (en) * 1988-06-22 1993-10-26 Board Of Regents, The University Of Texas System Freezing/perfusion microscope stage

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5257128A (en) * 1988-06-22 1993-10-26 Board Of Regents, The University Of Texas System Freezing/perfusion microscope stage

Also Published As

Publication number Publication date
WO2007123684A2 (en) 2007-11-01

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