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WO2006091892A3 - Methods of producing recombinant proteins - Google Patents

Methods of producing recombinant proteins Download PDF

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Publication number
WO2006091892A3
WO2006091892A3 PCT/US2006/006741 US2006006741W WO2006091892A3 WO 2006091892 A3 WO2006091892 A3 WO 2006091892A3 US 2006006741 W US2006006741 W US 2006006741W WO 2006091892 A3 WO2006091892 A3 WO 2006091892A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
host cell
recombinant protein
systems
recombinant proteins
Prior art date
Application number
PCT/US2006/006741
Other languages
French (fr)
Other versions
WO2006091892A2 (en
Inventor
Alan Greener
Marie Callahan
Xiaodong Wang
Original Assignee
Gene Therapy Systems Inc
Alan Greener
Marie Callahan
Xiaodong Wang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gene Therapy Systems Inc, Alan Greener, Marie Callahan, Xiaodong Wang filed Critical Gene Therapy Systems Inc
Publication of WO2006091892A2 publication Critical patent/WO2006091892A2/en
Publication of WO2006091892A3 publication Critical patent/WO2006091892A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Described herein are methods of producing a recombinant protein. The methods can include providing a host cell comprising a recombinant nucleic acid construct, wherein said recombinant nucleic acid construct encodes a recombinant protein and contacting the host cell with a first compound to which the host cell is sensitive. The methods can also include harvesting the host cells and recombinant protein from said host cell. The methods can be practiced with in vitro systems as well. Also described are systems and kits related to the methods. The methods systems and kits can be used to produce soluble recombinant protein and to increase the level of a recombinant protein relative to cellular protein in a host cell
PCT/US2006/006741 2005-02-25 2006-02-23 Methods of producing recombinant proteins WO2006091892A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US65664905P 2005-02-25 2005-02-25
US60/656,649 2005-02-25
US71085905P 2005-08-24 2005-08-24
US60/710,859 2005-08-24

Publications (2)

Publication Number Publication Date
WO2006091892A2 WO2006091892A2 (en) 2006-08-31
WO2006091892A3 true WO2006091892A3 (en) 2006-11-30

Family

ID=36928087

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/006741 WO2006091892A2 (en) 2005-02-25 2006-02-23 Methods of producing recombinant proteins

Country Status (1)

Country Link
WO (1) WO2006091892A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201101794D0 (en) 2011-02-02 2011-03-16 Fermentas Uab Protein production
US20210040472A1 (en) 2019-08-09 2021-02-11 Nutcracker Therapeutics, Inc. Methods and apparatuses for manufacturing for removing material from a therapeutic composition
WO2024028340A1 (en) * 2022-08-02 2024-02-08 Basf Se Stabilized protein production process using bacillus host cells with salt feed

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5716803A (en) * 1990-02-12 1998-02-10 Regeneron Pharmaceuticals, Inc. Recombinant protein production with reduced expression of selectable markers

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5716803A (en) * 1990-02-12 1998-02-10 Regeneron Pharmaceuticals, Inc. Recombinant protein production with reduced expression of selectable markers

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
WILLIAMS D.C. ET AL.: "Cytoplasmic Inclusion Bodies in Escherichia coli Producing Biosynthetic Human Insulin Proteins", SCIENCE, vol. 215, 5 February 1982 (1982-02-05), pages 687 - 689, XP003005330 *

Also Published As

Publication number Publication date
WO2006091892A2 (en) 2006-08-31

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