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WO2006040373A3 - Identificación de adn en alimentos crudos o procesados y piensos compuestos - Google Patents

Identificación de adn en alimentos crudos o procesados y piensos compuestos Download PDF

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Publication number
WO2006040373A3
WO2006040373A3 PCT/ES2005/000486 ES2005000486W WO2006040373A3 WO 2006040373 A3 WO2006040373 A3 WO 2006040373A3 ES 2005000486 W ES2005000486 W ES 2005000486W WO 2006040373 A3 WO2006040373 A3 WO 2006040373A3
Authority
WO
WIPO (PCT)
Prior art keywords
compound feeds
origin
dna
processed foods
identification
Prior art date
Application number
PCT/ES2005/000486
Other languages
English (en)
French (fr)
Other versions
WO2006040373A2 (es
WO2006040373B1 (es
Inventor
Santa Cruz Jose Manue Bautista
Catalina Antonio Puyet
Meyer Susana Schoenhuth
Original Assignee
Univ Madrid Complutense
Santa Cruz Jose Manue Bautista
Catalina Antonio Puyet
Meyer Susana Schoenhuth
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Madrid Complutense, Santa Cruz Jose Manue Bautista, Catalina Antonio Puyet, Meyer Susana Schoenhuth filed Critical Univ Madrid Complutense
Publication of WO2006040373A2 publication Critical patent/WO2006040373A2/es
Publication of WO2006040373A3 publication Critical patent/WO2006040373A3/es
Publication of WO2006040373B1 publication Critical patent/WO2006040373B1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/02—Food

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La invención consiste en un procedimiento de identificación del origen del ADN presente en los productos alimenticios a analizar. Mediante este procedimiento se detecta y diferencia si en la composición se halla presente ADN de origen vegetal o animal y si, dentro de este último grupo, existe ADN de mamíferos, aves o peces. Para llevar a cabo el procedimiento, se diseñan 9 oligonucleótidos cebadores específicos. Estos oligonucleótidos han sido diseñados de forma que tras la amplificación mediante PCR múltiple se puede diferenciar la procedencia de los alimentos que integran el producto, mediante el tamaño de los fragmentos amplificados. Esta invención tiene aplicación en el correcto etiquetado de los alimentos procesados cuya composición se precise a nivel general, como puede ser la certificación de piensos compuestos para acuicultura (100% pescado), o para rumiantes (10% vegetal).
PCT/ES2005/000486 2004-09-09 2005-09-08 Identificación de adn en alimentos crudos o procesados y piensos compuestos WO2006040373A2 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ES200402166A ES2249176B1 (es) 2004-09-09 2004-09-09 Identificacion de adn en alimentos crudos o procesados y piensos compuestos.
ESP200402166 2004-09-09

Publications (3)

Publication Number Publication Date
WO2006040373A2 WO2006040373A2 (es) 2006-04-20
WO2006040373A3 true WO2006040373A3 (es) 2006-08-24
WO2006040373B1 WO2006040373B1 (es) 2006-09-21

Family

ID=36101258

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/ES2005/000486 WO2006040373A2 (es) 2004-09-09 2005-09-08 Identificación de adn en alimentos crudos o procesados y piensos compuestos

Country Status (2)

Country Link
ES (1) ES2249176B1 (es)
WO (1) WO2006040373A2 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106434984A (zh) * 2016-11-25 2017-02-22 淮海工学院 一种检测大分子水产胶原蛋白或明胶的pcr引物及方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0807690A1 (en) * 1996-05-13 1997-11-19 Karl-Schmitz-Scholl-Fonds für Umweltrecht und Umweltpolitik e.V. Method and compositions for identification of species origin of caviar
WO1998008972A1 (de) * 1996-08-31 1998-03-05 Eberhard-Karls-Universität Tübingen Sequentielle hybridisierung von pilzzellen-dna sowie verfahren zum nachweisen von pilzzellen in klinischem material
WO2002077278A1 (en) * 2001-03-28 2002-10-03 Council Of Scientific And Industrial Research Universal primers for wildlife identification
WO2002101090A2 (fr) * 2001-06-13 2002-12-19 Centre National De La Recherche Scientifique Procede de determination de l'existence de melanges d'origines animales ou vegetales dans des substrats organiques

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0807690A1 (en) * 1996-05-13 1997-11-19 Karl-Schmitz-Scholl-Fonds für Umweltrecht und Umweltpolitik e.V. Method and compositions for identification of species origin of caviar
WO1998008972A1 (de) * 1996-08-31 1998-03-05 Eberhard-Karls-Universität Tübingen Sequentielle hybridisierung von pilzzellen-dna sowie verfahren zum nachweisen von pilzzellen in klinischem material
WO2002077278A1 (en) * 2001-03-28 2002-10-03 Council Of Scientific And Industrial Research Universal primers for wildlife identification
WO2002101090A2 (fr) * 2001-06-13 2002-12-19 Centre National De La Recherche Scientifique Procede de determination de l'existence de melanges d'origines animales ou vegetales dans des substrats organiques

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
DALMASSO A. ET AL.: "A multiplex PCR assay for the identification of animal species in feedstuffs", MOL. CELL PROBES, vol. 18, no. 2, April 2004 (2004-04-01), pages 81 - 87 *
TELETCHEA F. ET AL.: "Food and Forensic molecular identification: update and challenges", TRENDS IN BIOTECHNOLOGY, vol. 23, no. 7, July 2005 (2005-07-01), pages 359 - 36 *

Also Published As

Publication number Publication date
ES2249176A1 (es) 2006-03-16
ES2249176B1 (es) 2006-12-16
WO2006040373A2 (es) 2006-04-20
WO2006040373B1 (es) 2006-09-21

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