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WO2004072645A3 - Identification of organic compounds capable of modulating the activity of epithelial sodium ion channels - Google Patents

Identification of organic compounds capable of modulating the activity of epithelial sodium ion channels Download PDF

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Publication number
WO2004072645A3
WO2004072645A3 PCT/EP2004/001371 EP2004001371W WO2004072645A3 WO 2004072645 A3 WO2004072645 A3 WO 2004072645A3 EP 2004001371 W EP2004001371 W EP 2004001371W WO 2004072645 A3 WO2004072645 A3 WO 2004072645A3
Authority
WO
WIPO (PCT)
Prior art keywords
activity
sodium ion
ion channels
modulating
identification
Prior art date
Application number
PCT/EP2004/001371
Other languages
French (fr)
Other versions
WO2004072645A2 (en
Inventor
Henry Luke Danahay
Martin Gosling
Original Assignee
Novartis Ag
Novartis Pharma Gmbh
Henry Luke Danahay
Martin Gosling
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis Ag, Novartis Pharma Gmbh, Henry Luke Danahay, Martin Gosling filed Critical Novartis Ag
Publication of WO2004072645A2 publication Critical patent/WO2004072645A2/en
Publication of WO2004072645A3 publication Critical patent/WO2004072645A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method of identifying a substance that modulates the activity of epithelial sodium ion channels and as such may be suitable for use in the treatment of inflammatory diseases. The method involves treating a sample of epithelial cells with a candidate substance and a fluorescent membrane potential sensitive dye and measuring any changes in fluorescence.
PCT/EP2004/001371 2003-02-14 2004-02-13 Identification of organic compounds capable of modulating the activity of epithelial sodium ion channels WO2004072645A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0303506A GB0303506D0 (en) 2003-02-14 2003-02-14 Organic compounds
GB0303506.0 2003-02-14

Publications (2)

Publication Number Publication Date
WO2004072645A2 WO2004072645A2 (en) 2004-08-26
WO2004072645A3 true WO2004072645A3 (en) 2004-10-28

Family

ID=9953081

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2004/001371 WO2004072645A2 (en) 2003-02-14 2004-02-13 Identification of organic compounds capable of modulating the activity of epithelial sodium ion channels

Country Status (2)

Country Link
GB (1) GB0303506D0 (en)
WO (1) WO2004072645A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2658596A1 (en) * 2006-07-21 2008-01-24 Unilever Plc Method for screening an enhancer or a masker of salty taste
US9534035B2 (en) 2008-01-25 2017-01-03 Chromocell Corporation Cell lines expressing ENaC and methods using them

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000023023A1 (en) * 1998-10-20 2000-04-27 The University Of North Carolina At Chapel Hill Methods of hydrating mucosal surfaces
DE10006309A1 (en) * 2000-02-12 2001-08-23 Aventis Pharma Gmbh Method for the identification of substances that modulate the activity of hyperpolarization-activated cation channels
WO2002087306A2 (en) * 2001-05-01 2002-11-07 Senomyx, Inc. High throughput cell-based assay for monitoring sodium channel activity and discovery of salty taste modulating compounds

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000023023A1 (en) * 1998-10-20 2000-04-27 The University Of North Carolina At Chapel Hill Methods of hydrating mucosal surfaces
DE10006309A1 (en) * 2000-02-12 2001-08-23 Aventis Pharma Gmbh Method for the identification of substances that modulate the activity of hyperpolarization-activated cation channels
WO2002087306A2 (en) * 2001-05-01 2002-11-07 Senomyx, Inc. High throughput cell-based assay for monitoring sodium channel activity and discovery of salty taste modulating compounds

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
HOWARD D P J ET AL: "L-arginine effects on Na+ transport in M-1 mouse cortical collecting duct cells: A cationic amino acid absorbing epithelium", JOURNAL OF MEMBRANE BIOLOGY, vol. 180, no. 2, 15 March 2001 (2001-03-15), pages 111 - 121, XP002285851, ISSN: 0022-2631 *
KORBMACHER CHRISTOPH ET AL: "Whole-cell currents in single and confluent M-1 mouse cortical collecting duct cells", JOURNAL OF GENERAL PHYSIOLOGY, vol. 102, no. 4, 1993, pages 761 - 793, XP002285852, ISSN: 0022-1295 *
LEVER J E: "VARIANT MDCK KIDNEY EPITHELIAL CELLS ALTERED IN RESPONSE TO INDUCERS OF DOME FORMATION AND DIFFERENTIATION", JOURNAL OF CELLULAR PHYSIOLOGY, vol. 122, no. 1, 1985, pages 45 - 52, XP008032149, ISSN: 0021-9541 *

Also Published As

Publication number Publication date
GB0303506D0 (en) 2003-03-19
WO2004072645A2 (en) 2004-08-26

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