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WO2002046457A3 - Verfahren zur codierung von hybriddisierungssonden - Google Patents

Verfahren zur codierung von hybriddisierungssonden Download PDF

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Publication number
WO2002046457A3
WO2002046457A3 PCT/EP2001/014391 EP0114391W WO0246457A3 WO 2002046457 A3 WO2002046457 A3 WO 2002046457A3 EP 0114391 W EP0114391 W EP 0114391W WO 0246457 A3 WO0246457 A3 WO 0246457A3
Authority
WO
WIPO (PCT)
Prior art keywords
overhang
encoders
dna fragment
attached
encoder
Prior art date
Application number
PCT/EP2001/014391
Other languages
English (en)
French (fr)
Other versions
WO2002046457A2 (de
Inventor
Achim Fischer
Dieter Newrzella
Original Assignee
Axaron Bioscience Ag
Achim Fischer
Dieter Newrzella
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Axaron Bioscience Ag, Achim Fischer, Dieter Newrzella filed Critical Axaron Bioscience Ag
Priority to AU2002216078A priority Critical patent/AU2002216078A1/en
Publication of WO2002046457A2 publication Critical patent/WO2002046457A2/de
Publication of WO2002046457A3 publication Critical patent/WO2002046457A3/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Die Erfindung betrifft ein Verfahren zur Identifikation von Nukleinsäuren, gekennzeichnet durch die folgenden Schritte: a) Bereitstellung mindestens eines DNA-Fragments mit einem überhängenden Ende bekannter Länge und unbekannter Sequenz; b) Befestigung eines bestimmten Encoders aus einer Mischung von Encodern mit ebenfalls überhängendem Ende an dem überhängenden Ende unbekannter Sequenz des DNA-Fragments, wobei das überhängende Ende des befestigten Encoders und das überhängende Ende unbekannter Sequenz des DNA-Fragments vollständig komplementär zueinander sind; c) gegebenenfalls Abtrennung derjenigen Encoder, welche in Schritt (b) an keinem DNA-Fragment befestigt wurden; d) Markierung des Befestigungsprodukts aus Schritt (b), sofern nicht bereits markierte Encoder zur Befestigung eingesetzt wurden; e) Hybridisierung des markierten Befestigungsprodukts mit einem Array von Oligonukleotiden, welche im wesentlichen komplementär zu einem der Stränge aller möglichen Encoder aus der Mischung von Encodern oder einer Auswahl hiervon sind.
PCT/EP2001/014391 2000-12-07 2001-12-07 Verfahren zur codierung von hybriddisierungssonden WO2002046457A2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002216078A AU2002216078A1 (en) 2000-12-07 2001-12-07 Method for encoding hybridization probes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10060827.2 2000-12-07
DE10060827A DE10060827A1 (de) 2000-12-07 2000-12-07 Verfahren zur Codierung von Hybridisierungssonden

Publications (2)

Publication Number Publication Date
WO2002046457A2 WO2002046457A2 (de) 2002-06-13
WO2002046457A3 true WO2002046457A3 (de) 2002-12-19

Family

ID=7666146

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2001/014391 WO2002046457A2 (de) 2000-12-07 2001-12-07 Verfahren zur codierung von hybriddisierungssonden

Country Status (3)

Country Link
AU (1) AU2002216078A1 (de)
DE (1) DE10060827A1 (de)
WO (1) WO2002046457A2 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115058469A (zh) * 2022-04-25 2022-09-16 深圳大学 一种短dna片段3’端生物素标记的方法
CN118072835B (zh) * 2024-04-19 2024-09-17 宁波甬恒瑶瑶智能科技有限公司 基于机器学习的生物信息学数据处理方法、系统及介质

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5710000A (en) * 1994-09-16 1998-01-20 Affymetrix, Inc. Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping
WO1998010095A1 (en) * 1996-09-05 1998-03-12 Brax Genomics Limited Characterising dna
WO2000009756A1 (en) * 1998-08-17 2000-02-24 The Perkin-Elmer Corporation Adapter directed expression analysis
WO2000060124A2 (en) * 1999-04-06 2000-10-12 Yale University Fixed address analysis of sequence tags
WO2001012855A2 (en) * 1999-08-13 2001-02-22 Yale University Binary encoded sequence tags

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5710000A (en) * 1994-09-16 1998-01-20 Affymetrix, Inc. Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping
WO1998010095A1 (en) * 1996-09-05 1998-03-12 Brax Genomics Limited Characterising dna
WO2000009756A1 (en) * 1998-08-17 2000-02-24 The Perkin-Elmer Corporation Adapter directed expression analysis
WO2000060124A2 (en) * 1999-04-06 2000-10-12 Yale University Fixed address analysis of sequence tags
WO2001012855A2 (en) * 1999-08-13 2001-02-22 Yale University Binary encoded sequence tags

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BRENNER SYDNEY ET AL: "Gene expression analysis by massively parallel signature sequencing (MPSS) on microbead arrays", NATURE BIOTECHNOLOGY, NATURE PUB. CO, NEW YORK, NY, US, vol. 18, no. 6, June 2000 (2000-06-01), pages 630 - 634, XP002199492, ISSN: 1087-0156 *

Also Published As

Publication number Publication date
DE10060827A1 (de) 2002-06-13
WO2002046457A2 (de) 2002-06-13
AU2002216078A1 (en) 2002-06-18

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