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WO2001060975A3 - Methodologies de disruption genique destinees a la decouverte de medicaments cibles - Google Patents

Methodologies de disruption genique destinees a la decouverte de medicaments cibles Download PDF

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Publication number
WO2001060975A3
WO2001060975A3 PCT/US2001/005551 US0105551W WO0160975A3 WO 2001060975 A3 WO2001060975 A3 WO 2001060975A3 US 0105551 W US0105551 W US 0105551W WO 0160975 A3 WO0160975 A3 WO 0160975A3
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WO
WIPO (PCT)
Prior art keywords
genes
essential
gene
allele
development
Prior art date
Application number
PCT/US2001/005551
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English (en)
Other versions
WO2001060975A2 (fr
Inventor
Terry Roemer
Bo Jiang
Charles Boone
Howard Bussey
Original Assignee
Elitra Pharmaceuticals Inc
Terry Roemer
Charles Boone
Bussy Howard
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Elitra Pharmaceuticals Inc, Terry Roemer, Charles Boone, Bussy Howard filed Critical Elitra Pharmaceuticals Inc
Priority to JP2001560347A priority Critical patent/JP2003523197A/ja
Priority to NZ521037A priority patent/NZ521037A/en
Priority to EP01916144A priority patent/EP1292668A2/fr
Priority to KR1020027010815A priority patent/KR20020097180A/ko
Priority to AU2001243204A priority patent/AU2001243204A1/en
Priority to CA002398861A priority patent/CA2398861A1/fr
Publication of WO2001060975A2 publication Critical patent/WO2001060975A2/fr
Publication of WO2001060975A3 publication Critical patent/WO2001060975A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/10—Antimycotics
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/40—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Candida
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Oncology (AREA)
  • Botany (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Communicable Diseases (AREA)
  • Public Health (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne des procédés et compositions permettant de déterminer de manière expérimentale si un quelconque gène du génome d'un organisme pathogène diploïde est essentiel et s'il est nécessaire à la virulence ou au pouvoir pathogène. Ces procédés consistent à construire des mutants génétiques dans lesquels un allèle d'un gène spécifique est inactivé tandis que l'autre allèle du gène est placé dans des conditions d'éventuelle expression. L'identification de gènes essentiels et de gènes critiques quant au développement d'infections virulentes constitue une base de développement du criblage de nouveaux médicaments dirigés contre ces organismes pathogènes. L'invention concerne encore des gènes ∫i⊃Candida albicans∫/i⊃ qui se sont révélés être essentiels dans le criblage de médicaments et constituent des cibles potentielles à cette fin. On peut utiliser la séquence nucléotidique de ces gènes cibles à des fins de découverte de médicaments, telle que l'expression de la protéine de recombinaison, le dosage d'hybridation et la construction d'ensembles d'acides nucléiques. L'invention concerne enfin l'utilisation de protéines, codées par les gènes essentiels, et de cellules modifiées génétiquement et comprenant des allèles modifiés de gènes essentiels, dans divers procédés de criblage.
PCT/US2001/005551 2000-02-18 2001-02-20 Methodologies de disruption genique destinees a la decouverte de medicaments cibles WO2001060975A2 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
JP2001560347A JP2003523197A (ja) 2000-02-18 2001-02-20 薬物標的探索のための遺伝子破壊法
NZ521037A NZ521037A (en) 2000-02-18 2001-02-20 Gene disruption methodologies for drug target discovery
EP01916144A EP1292668A2 (fr) 2000-02-18 2001-02-20 Methodologies de disruption genique destinees a la decouverte de medicaments cibles
KR1020027010815A KR20020097180A (ko) 2000-02-18 2001-02-20 약물 표적 발견을 위한 유전자 분열 방법
AU2001243204A AU2001243204A1 (en) 2000-02-18 2001-02-20 Gene disruption methodologies for drug target discovery
CA002398861A CA2398861A1 (fr) 2000-02-18 2001-02-20 Methodologies de disruption genique destinees a la decouverte de medicaments cibles

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US18353400P 2000-02-18 2000-02-18
US60/183,534 2000-02-18

Publications (2)

Publication Number Publication Date
WO2001060975A2 WO2001060975A2 (fr) 2001-08-23
WO2001060975A3 true WO2001060975A3 (fr) 2002-04-25

Family

ID=22673211

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/005551 WO2001060975A2 (fr) 2000-02-18 2001-02-20 Methodologies de disruption genique destinees a la decouverte de medicaments cibles

Country Status (7)

Country Link
EP (1) EP1292668A2 (fr)
JP (1) JP2003523197A (fr)
KR (1) KR20020097180A (fr)
AU (1) AU2001243204A1 (fr)
CA (1) CA2398861A1 (fr)
NZ (1) NZ521037A (fr)
WO (1) WO2001060975A2 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030180953A1 (en) * 2000-12-29 2003-09-25 Elitra Pharmaceuticals, Inc. Gene disruption methodologies for drug target discovery
WO2003010333A2 (fr) * 2001-07-24 2003-02-06 Affinium Pharmaceuticals Inc. Methodes de disruption genique et leurs utilisations
WO2003091418A2 (fr) 2002-04-26 2003-11-06 Bristol-Myers Squibb Company Nouveaux polynucleotides fongiques essentiels, polypeptides et techniques d'utilisation
GB0228702D0 (en) * 2002-12-09 2003-01-15 Oxford Glycosciences Uk Ltd Novel therapeutic target
GB0228694D0 (en) * 2002-12-09 2003-01-15 Oxford Glycosciences Uk Ltd Novel threapeutic target
GB0228697D0 (en) * 2002-12-09 2003-01-15 Oxford Glycosciences Uk Ltd Novel therapeutic target
GB0228704D0 (en) * 2002-12-09 2003-01-15 Oxford Glycosciences Uk Ltd Novel therapeutic target
EP1466974A1 (fr) * 2003-04-11 2004-10-13 Max-Delbrück-Centrum Für Molekulare Medizin Mutagenèse dirigé inductible grâce au sauvetage conditionel d'un gène
CN108707554B (zh) * 2018-05-21 2021-06-29 河北省农林科学院植物保护研究所 一对玉蜀黍黑粉菌组合及其应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997016540A1 (fr) * 1995-11-01 1997-05-09 Millennium Pharmaceuticals, Inc. Chitine synthase 1
WO1999031269A2 (fr) * 1997-12-12 1999-06-24 Mcgill University Nouveau candida albicans kre9 et ses utilisations
WO2000053781A1 (fr) * 1999-03-11 2000-09-14 Gpc Biotech, Inc. Generation de mutants de levure conditionnels, techniques et reactifs s'y rapportant

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997016540A1 (fr) * 1995-11-01 1997-05-09 Millennium Pharmaceuticals, Inc. Chitine synthase 1
WO1999031269A2 (fr) * 1997-12-12 1999-06-24 Mcgill University Nouveau candida albicans kre9 et ses utilisations
WO2000053781A1 (fr) * 1999-03-11 2000-09-14 Gpc Biotech, Inc. Generation de mutants de levure conditionnels, techniques et reactifs s'y rapportant

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
GARI ELOI ET AL: "A set of vectors with a tetracycline-regulatable promoter system for modulated gene expression in Saccharomyces cerevisiae", YEAST, CHICHESTER, SUSSEX, GB, vol. 13, no. 9, 1997, pages 837 - 848, XP000926574, ISSN: 0749-503X *
GOW N A R ET AL: "GENES ASSOCIATED WITH DIMORPHISM AND VIRULENCE OF CANDIDA ALBICANS", CANADIAN JOURNAL OF BOTANY, NATIONAL RESEARCH COUNCIL, OTTAWA, CA, vol. 73, no. SUPPL 1, 1995, pages S335 - S342, XP000926595, ISSN: 0008-4026 *
MAO YUXIN ET AL: "Overexpression of a dominant-negative allele of SEC4 inhibits growth and protein secretion in Candida albicans.", JOURNAL OF BACTERIOLOGY, vol. 181, no. 23, December 1999 (1999-12-01), pages 7235 - 7242, XP002180711, ISSN: 0021-9193 *
MENDOZA A ET AL: "Translation elongation factor 2 is encoded by a single essential gene in Candida albicans", GENE, ELSEVIER BIOMEDICAL PRESS. AMSTERDAM, NL, vol. 229, no. 1-2, 18 March 1999 (1999-03-18), pages 183 - 191, XP004161173, ISSN: 0378-1119 *
MIO ET AL: "Cloning of the Candida albicans homolog of Saccharomyces cerevisiae GSC1/ FKS1 and its involvement in beta-1,3-glucan synthesis", JOURNAL OF BACTERIOLOGY, WASHINGTON, DC, US, vol. 179, no. 13, July 1997 (1997-07-01), pages 4096 - 4105, XP002115088, ISSN: 0021-9193 *
MIO TOSHIYUKI ET AL: "Isolation of the Candida albicans homologs of Saccharomyces cerevisiae KRE6 and SKN1: Expression and physiological function", JOURNAL OF BACTERIOLOGY, WASHINGTON, DC, US, vol. 179, no. 7, 1997, pages 2363 - 2372, XP002156281, ISSN: 0021-9193 *

Also Published As

Publication number Publication date
AU2001243204A1 (en) 2001-08-27
CA2398861A1 (fr) 2001-08-23
JP2003523197A (ja) 2003-08-05
NZ521037A (en) 2004-09-24
WO2001060975A2 (fr) 2001-08-23
EP1292668A2 (fr) 2003-03-19
KR20020097180A (ko) 2002-12-31

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