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WO2001042508A3 - Methods and compositions relating to electrical detection of nucleic acid reactions - Google Patents

Methods and compositions relating to electrical detection of nucleic acid reactions Download PDF

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Publication number
WO2001042508A3
WO2001042508A3 PCT/US2000/033497 US0033497W WO0142508A3 WO 2001042508 A3 WO2001042508 A3 WO 2001042508A3 US 0033497 W US0033497 W US 0033497W WO 0142508 A3 WO0142508 A3 WO 0142508A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
nucleic acid
electrical detection
relates
interactions
Prior art date
Application number
PCT/US2000/033497
Other languages
French (fr)
Other versions
WO2001042508A2 (en
WO2001042508A9 (en
Inventor
Vi-En Choong
Sean Gallagher
Mike Gaskin
Changming Li
George Maracas
Song Shi
Original Assignee
Motorola Inc
Choong Vi En
Sean Gallagher
Mike Gaskin
Changming Li
George Maracas
Song Shi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/458,533 external-priority patent/US20020051975A1/en
Priority claimed from US09/459,685 external-priority patent/US6518024B2/en
Application filed by Motorola Inc, Choong Vi En, Sean Gallagher, Mike Gaskin, Changming Li, George Maracas, Song Shi filed Critical Motorola Inc
Priority to CA002393733A priority Critical patent/CA2393733A1/en
Priority to EP00993326A priority patent/EP1238114A2/en
Priority to JP2001544379A priority patent/JP2003516165A/en
Priority to US10/149,319 priority patent/US20030209432A1/en
Priority to AU29072/01A priority patent/AU2907201A/en
Publication of WO2001042508A2 publication Critical patent/WO2001042508A2/en
Publication of WO2001042508A3 publication Critical patent/WO2001042508A3/en
Publication of WO2001042508A9 publication Critical patent/WO2001042508A9/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

This invention relates to the detection of molecular interactions between biological molecules. Specifically, the invention relates to electrical detection of interactions such as hybridization between nucleic acids or peptide antigen-antibody interactions using arrays of peptides or oligonucleotides. In particular, the invention relates to an apparatus and methods for detecting nucleic acid hybridization or peptide binding using electronic methods including AC impedance. In some embodiments, no electrochemical or other label moieties are used. In others, electrochemically active labels are used to detect reactions on hydrogel arrays, including genotyping reactions such as the single base extension reaction.
PCT/US2000/033497 1999-12-09 2000-12-11 Methods and compositions relating to electrical detection of nucleic acid reactions WO2001042508A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA002393733A CA2393733A1 (en) 1999-12-09 2000-12-11 Methods and compositions relating to electrical detection of nucleic acid reactions
EP00993326A EP1238114A2 (en) 1999-12-09 2000-12-11 Methods and compositions relating to electrical detection of nucleic acid reactions
JP2001544379A JP2003516165A (en) 1999-12-09 2000-12-11 Methods and compositions for electrical detection of nucleic acid reactions
US10/149,319 US20030209432A1 (en) 2000-12-11 2000-12-11 Methods and compositions relating to electrical detection of nucleic acid reactions
AU29072/01A AU2907201A (en) 1999-12-09 2000-12-11 Methods and compositions relating to electrical detection of nucleic acid reactions

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US45850199A 1999-12-09 1999-12-09
US09/458,533 US20020051975A1 (en) 1999-12-09 1999-12-09 Reporterless genosensors using electrical detection methods
US09/458,501 1999-12-09
US09/458,533 1999-12-09
US09/459,685 1999-12-13
US09/459,685 US6518024B2 (en) 1999-12-13 1999-12-13 Electrochemical detection of single base extension

Publications (3)

Publication Number Publication Date
WO2001042508A2 WO2001042508A2 (en) 2001-06-14
WO2001042508A3 true WO2001042508A3 (en) 2002-03-14
WO2001042508A9 WO2001042508A9 (en) 2002-05-30

Family

ID=27412734

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/033497 WO2001042508A2 (en) 1999-12-09 2000-12-11 Methods and compositions relating to electrical detection of nucleic acid reactions

Country Status (5)

Country Link
EP (1) EP1238114A2 (en)
JP (1) JP2003516165A (en)
AU (1) AU2907201A (en)
CA (1) CA2393733A1 (en)
WO (1) WO2001042508A2 (en)

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US6071699A (en) 1996-06-07 2000-06-06 California Institute Of Technology Nucleic acid mediated electron transfer
US6824669B1 (en) * 2000-02-17 2004-11-30 Motorola, Inc. Protein and peptide sensors using electrical detection methods
GB0118931D0 (en) * 2001-08-02 2001-09-26 Oxford Biosensors Ltd Voltammetric ion-selective biosensor
DE10210051B4 (en) 2002-03-07 2005-10-13 Siemens Ag Device for the electrochemical detection of a nucleotide sequence, analysis cassette for such a device and method for producing such an analysis cassette
DE10224567B4 (en) * 2002-06-03 2014-10-23 Boehringer Ingelheim Vetmedica Gmbh Sensor arrangement and method for operating a sensor arrangement
DE10228260A1 (en) * 2002-06-25 2004-01-22 Bayer Ag Method and device for the impedimetric detection of one or more analytes in a sample
GB2391867A (en) * 2002-08-13 2004-02-18 Smartbead Technologies Ltd Analysis system using coded supports
KR100858080B1 (en) * 2002-11-12 2008-09-10 삼성전자주식회사 A method for detecting a PCR product by measuring a electrical signal
AU2003266711A1 (en) * 2002-11-14 2004-06-03 Cosel Co., Ltd. Method of detecting hybridization
DE10259819B4 (en) 2002-12-19 2006-07-13 Siemens Ag Method for PCR amplification and detection of nucleotide sequences
WO2005016115A2 (en) 2003-01-23 2005-02-24 Montana State University Biosensors utilizing dendrimer-immobilized ligands and their use thereof
AT413214B (en) * 2003-01-24 2005-12-15 Sy Lab Vgmbh MEASUREMENT ARRANGEMENT AND METHOD FOR DETECTING A DNA SEQUENCE
EP1608775A1 (en) * 2003-03-05 2005-12-28 University of Saskatchewan Technologies Inc. Nucleic acid mismatch detection
WO2005001122A2 (en) * 2003-06-27 2005-01-06 Adnavance Technologies, Inc. Electrochemical detection of dna binding
DE10327756B4 (en) * 2003-06-18 2005-12-29 november Aktiengesellschaft Gesellschaft für Molekulare Medizin Method for real-time quantification of a nucleic acid
KR100590569B1 (en) * 2004-02-14 2006-06-15 삼성전자주식회사 Method for real-time detection of Polymerase Chain Reaction
US20080227651A1 (en) * 2004-03-25 2008-09-18 Yanxiu Zhou Cyclic voltammetry (CV) for identifying genomic sequence variations and detecting mismatch base pairs, such as single nucleotide polymorphisms
WO2005093418A1 (en) * 2004-03-26 2005-10-06 Toto, Ltd. Method of specifically detecting test substance by using photocurrent and electrodes, measurement cell, measurement device and buffer solution to be used therefor
US8097134B2 (en) 2004-04-01 2012-01-17 Nanyang Technological University Addressable chem/bio chip array
KR100613612B1 (en) * 2004-04-27 2006-08-18 삼성전자주식회사 Apparatus and method of detecting bio bonding using circuit of inductor and capacitor
WO2006079009A2 (en) 2005-01-21 2006-07-27 University Of Rochester Methods for separating short single-stranded nucleic acid from long single- and double-stranded nucleic acid, and associated biomolecular assays
WO2009041917A1 (en) * 2007-09-28 2009-04-02 Agency For Science, Technology And Research Method of electrically detecting a nucleic acid molecule
NZ610129A (en) 2010-10-04 2014-08-29 Genapsys Inc Systems and methods for automated reusable parallel biological reactions
US9399217B2 (en) 2010-10-04 2016-07-26 Genapsys, Inc. Chamber free nanoreactor system
US9184099B2 (en) 2010-10-04 2015-11-10 The Board Of Trustees Of The Leland Stanford Junior University Biosensor devices, systems and methods therefor
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
US8585973B2 (en) 2011-05-27 2013-11-19 The Board Of Trustees Of The Leland Stanford Junior University Nano-sensor array
EP2785868B1 (en) 2011-12-01 2017-04-12 Genapsys Inc. Systems and methods for high efficiency electronic sequencing and detection
US9809852B2 (en) 2013-03-15 2017-11-07 Genapsys, Inc. Systems and methods for biological analysis
EP3792921A1 (en) 2013-12-11 2021-03-17 Genapsys, Inc. Systems and methods for biological analysis and computation
EP3556864B1 (en) 2014-04-18 2020-12-09 Genapsys, Inc. Methods and systems for nucleic acid amplification
EP3488017A4 (en) 2016-07-20 2020-02-26 Genapsys Inc. Systems and methods for nucleic acid sequencing
SG11202002516WA (en) 2017-09-21 2020-04-29 Genapsys Inc Systems and methods for nucleic acid sequencing

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EP0226470A2 (en) * 1985-12-13 1987-06-24 Unilever Plc Materials and methods for microchemical testing
EP0372911A2 (en) * 1988-12-05 1990-06-13 CHIRANA Vyzkumny ustav zdravotnicke techniky koncernova ucelova organizace Recoverable enzyme sensor with replacement membrane system
US5001048A (en) * 1987-06-05 1991-03-19 Aurthur D. Little, Inc. Electrical biosensor containing a biological receptor immobilized and stabilized in a protein film
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US5001048A (en) * 1987-06-05 1991-03-19 Aurthur D. Little, Inc. Electrical biosensor containing a biological receptor immobilized and stabilized in a protein film
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US5972692A (en) * 1990-09-28 1999-10-26 Kabushiki Kaisha Toshiba Gene detection method
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US5770721A (en) * 1993-08-11 1998-06-23 University Of Chicago Method of manufacturing a matrix for the detection of mismatches
WO1996006946A1 (en) * 1994-08-26 1996-03-07 Igen, Inc. Biosensor for and method of electrogenerated chemiluminescent detection of nucleic acid adsorbed to a solid surface
WO1997012030A1 (en) * 1995-09-27 1997-04-03 Nanogen, Inc. Apparatus and methods for active programmable matrix devices
WO1998012539A1 (en) * 1996-09-17 1998-03-26 Meso Scale Technologies, Llc Multi-array, multi-specific electrochemiluminescence testing
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WO1999007879A1 (en) * 1997-08-12 1999-02-18 University Of Southern California Electrochemical reporter system for detecting analytical immunoassay and molecular biology procedures
WO1999015893A1 (en) * 1997-09-22 1999-04-01 Aventis Research & Technologies Gmbh & Co. Kg Addressable modular recognition system, production mode and use
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Also Published As

Publication number Publication date
EP1238114A2 (en) 2002-09-11
WO2001042508A2 (en) 2001-06-14
CA2393733A1 (en) 2001-06-14
JP2003516165A (en) 2003-05-13
AU2907201A (en) 2001-06-18
WO2001042508A9 (en) 2002-05-30

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