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WO2000074635A3 - Delivery of single stranded dna for expression - Google Patents

Delivery of single stranded dna for expression Download PDF

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Publication number
WO2000074635A3
WO2000074635A3 PCT/US2000/040134 US0040134W WO0074635A3 WO 2000074635 A3 WO2000074635 A3 WO 2000074635A3 US 0040134 W US0040134 W US 0040134W WO 0074635 A3 WO0074635 A3 WO 0074635A3
Authority
WO
WIPO (PCT)
Prior art keywords
single stranded
expression
delivery
stranded dna
gene
Prior art date
Application number
PCT/US2000/040134
Other languages
French (fr)
Other versions
WO2000074635A2 (en
Inventor
Jon A Wolff
Original Assignee
Mirus Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mirus Corp filed Critical Mirus Corp
Priority to EP00938285A priority Critical patent/EP1246532A2/en
Publication of WO2000074635A2 publication Critical patent/WO2000074635A2/en
Publication of WO2000074635A3 publication Critical patent/WO2000074635A3/en

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Public Health (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Veterinary Medicine (AREA)
  • Plant Pathology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Microbiology (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

Methods are described for generating and delivering a single stranded nucleic acid capable of expressing a gene. The single stranded nucleic acid is delivered to the host cell, where it transfects a host cell resulting in expression of the encoded gene for gene therapy purposes.
PCT/US2000/040134 1999-06-07 2000-06-07 Delivery of single stranded dna for expression WO2000074635A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP00938285A EP1246532A2 (en) 1999-06-07 2000-06-07 Delivery of single stranded dna for expression

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13785599P 1999-06-07 1999-06-07
US60/137,855 1999-06-07

Publications (2)

Publication Number Publication Date
WO2000074635A2 WO2000074635A2 (en) 2000-12-14
WO2000074635A3 true WO2000074635A3 (en) 2002-08-08

Family

ID=22479341

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/040134 WO2000074635A2 (en) 1999-06-07 2000-06-07 Delivery of single stranded dna for expression

Country Status (2)

Country Link
EP (1) EP1246532A2 (en)
WO (1) WO2000074635A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070173465A9 (en) * 1995-10-11 2007-07-26 Monahan Sean D Expression of zeta negative and zeta positive nucleic acids using a dystrophin gene
KR100397275B1 (en) * 2001-03-08 2003-09-17 주식회사 웰진 Novel high-throughput system for functional genomics using unidirectional antisense cDNA library
EP2325193A3 (en) * 2001-11-02 2012-05-02 Insert Therapeutics, Inc. Methods and compositions for therapeutic use of RNA interference
CN112195193A (en) * 2020-09-30 2021-01-08 上海交通大学 Application of single-stranded DNA in gene transfection

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5532001A (en) * 1993-07-07 1996-07-02 Trustees Of Boston University Stimulation of tanning by DNA fragments or single-stranded DNA
US5580859A (en) * 1989-03-21 1996-12-03 Vical Incorporated Delivery of exogenous DNA sequences in a mammal
US5705385A (en) * 1995-06-07 1998-01-06 Inex Pharmaceuticals Corporation Lipid-nucleic acid particles prepared via a hydrophobic lipid-nucleic acid complex intermediate and use for gene transfer
US5843742A (en) * 1994-12-16 1998-12-01 Avigen Incorporated Adeno-associated derived vector systems for gene delivery and integration into target cells
US5874555A (en) * 1987-10-30 1999-02-23 California Institute Of Technology Triple helices and processes for making same
US20010007767A1 (en) * 1995-12-15 2001-07-12 Elazar Rabbani Novel property effecting and/or property exhibiting compositions for therapeutic and diagnostic uses

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5874555A (en) * 1987-10-30 1999-02-23 California Institute Of Technology Triple helices and processes for making same
US5580859A (en) * 1989-03-21 1996-12-03 Vical Incorporated Delivery of exogenous DNA sequences in a mammal
US5532001A (en) * 1993-07-07 1996-07-02 Trustees Of Boston University Stimulation of tanning by DNA fragments or single-stranded DNA
US5843742A (en) * 1994-12-16 1998-12-01 Avigen Incorporated Adeno-associated derived vector systems for gene delivery and integration into target cells
US5705385A (en) * 1995-06-07 1998-01-06 Inex Pharmaceuticals Corporation Lipid-nucleic acid particles prepared via a hydrophobic lipid-nucleic acid complex intermediate and use for gene transfer
US20010007767A1 (en) * 1995-12-15 2001-07-12 Elazar Rabbani Novel property effecting and/or property exhibiting compositions for therapeutic and diagnostic uses

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LEWIS ET AL.: "Angiogenesis by gene therapy: a new horizon for myocardial revascularization?", CARDIOVASCULAR RES., vol. 35, 1 September 1997 (1997-09-01), pages 490 - 497, XP000916748 *
SEGOVIA ET AL.: "Astrocyte-specific expression of tyrosine hydroxylase after intracerebral gene transfer induces behavioral recovery in experimental parkinsonism", GENE THERAPY, vol. 5, December 1998 (1998-12-01), pages 1650 - 1655, XP002909757 *

Also Published As

Publication number Publication date
WO2000074635A2 (en) 2000-12-14
EP1246532A2 (en) 2002-10-09

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