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WO2000053735A3 - Methods for identifying modulators of synaptic plasticity and cell motility - Google Patents

Methods for identifying modulators of synaptic plasticity and cell motility Download PDF

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Publication number
WO2000053735A3
WO2000053735A3 PCT/IB2000/000505 IB0000505W WO0053735A3 WO 2000053735 A3 WO2000053735 A3 WO 2000053735A3 IB 0000505 W IB0000505 W IB 0000505W WO 0053735 A3 WO0053735 A3 WO 0053735A3
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WO
WIPO (PCT)
Prior art keywords
methods
cell motility
synaptic plasticity
candidate compound
identifying modulators
Prior art date
Application number
PCT/IB2000/000505
Other languages
French (fr)
Other versions
WO2000053735A9 (en
WO2000053735A2 (en
Inventor
Stephen Morris
Richard Murphy
James Fawcett
Timothy Kennedy
Colleen Mannit
Original Assignee
Univ Mcgill
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Filing date
Publication date
Application filed by Univ Mcgill filed Critical Univ Mcgill
Priority to AU36676/00A priority Critical patent/AU3667600A/en
Publication of WO2000053735A2 publication Critical patent/WO2000053735A2/en
Publication of WO2000053735A3 publication Critical patent/WO2000053735A3/en
Publication of WO2000053735A9 publication Critical patent/WO2000053735A9/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5011—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing antineoplastic activity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5029—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on cell motility
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5058—Neurological cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0393—Animal model comprising a reporter system for screening tests
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/475—Assays involving growth factors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Neurology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Neurosurgery (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention features a method for determining whether a candidate compound modulates the expression of a gene operably linked to a netrin promoter, including: a) providing a cell expressing a gene operably linked to a netrin promoter; b) contacting the cell with a candidate compound; c) detecting or measuring expression of the gene following contact of the cell with the candidate compound; and d) determining whether the candidate compound modulates the expression of the gene.
PCT/IB2000/000505 1999-03-10 2000-03-09 Methods for identifying modulators of synaptic plasticity and cell motility WO2000053735A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU36676/00A AU3667600A (en) 1999-03-10 2000-03-09 Methods for identifying compounds that modulate synaptic plasticity and cell motility

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12363699P 1999-03-10 1999-03-10
US60/123,636 1999-03-10

Publications (3)

Publication Number Publication Date
WO2000053735A2 WO2000053735A2 (en) 2000-09-14
WO2000053735A3 true WO2000053735A3 (en) 2000-12-28
WO2000053735A9 WO2000053735A9 (en) 2002-08-29

Family

ID=22409885

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2000/000505 WO2000053735A2 (en) 1999-03-10 2000-03-09 Methods for identifying modulators of synaptic plasticity and cell motility

Country Status (2)

Country Link
AU (1) AU3667600A (en)
WO (1) WO2000053735A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2573720A1 (en) 2004-07-14 2006-02-23 University Of Utah Research Foundation Netrin-related compositions and uses
JPWO2015025770A1 (en) * 2013-08-19 2017-03-02 国立大学法人大阪大学 Method for screening pain suppressing substance and pharmaceutical composition for preventing or treating pain

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5824775A (en) * 1996-04-19 1998-10-20 The Regents Of The University Of California Human netrin-1
US5939271A (en) * 1997-02-19 1999-08-17 The Regents Of The University Of California Netrin receptor
WO1999061585A1 (en) * 1998-05-22 1999-12-02 University Technology Corporation Assays for identifying anti-metastatic and neurite-promoting agents and uses thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5824775A (en) * 1996-04-19 1998-10-20 The Regents Of The University Of California Human netrin-1
US5939271A (en) * 1997-02-19 1999-08-17 The Regents Of The University Of California Netrin receptor
WO1999061585A1 (en) * 1998-05-22 1999-12-02 University Technology Corporation Assays for identifying anti-metastatic and neurite-promoting agents and uses thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
COLAMARINO, C. AND TESSIER-LAVIGNE, M.: "The axonal chemoattractant Netrin-1 is also a chemorepellent for trochlear motor axons", CELL, vol. 81, May 1995 (1995-05-01), pages 621 - 629, XP002147485 *
KENNEDY T E ET AL: "NETRINS ARE DIFFUSIBLE CHEMOTROPIC FACTORS FOR COMMISSURAL AXONS IN THE EMBRYONIC SPINAL CORD", CELL,US,CELL PRESS, CAMBRIDGE, NA, vol. 78, 12 August 1994 (1994-08-12), pages 425 - 435, XP002017868, ISSN: 0092-8674 *
MING, G-L. ET AL: "Phospholipase C-gamma and phosphoinositide 3-kinase mediate cytoplasmic signalling in nerve growth cone guidance", NEURON, vol. 23, May 1999 (1999-05-01), pages 139 - 148, XP000946117 *

Also Published As

Publication number Publication date
AU3667600A (en) 2000-09-28
WO2000053735A9 (en) 2002-08-29
WO2000053735A2 (en) 2000-09-14

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