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WO2000049410A3 - Lcm (laser capture microdissection) for cellular protein analysis - Google Patents

Lcm (laser capture microdissection) for cellular protein analysis Download PDF

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Publication number
WO2000049410A3
WO2000049410A3 PCT/US2000/004023 US0004023W WO0049410A3 WO 2000049410 A3 WO2000049410 A3 WO 2000049410A3 US 0004023 W US0004023 W US 0004023W WO 0049410 A3 WO0049410 A3 WO 0049410A3
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WO
WIPO (PCT)
Prior art keywords
cells
protein
analysis
methods
normal
Prior art date
Application number
PCT/US2000/004023
Other languages
French (fr)
Other versions
WO2000049410A2 (en
WO2000049410A9 (en
Inventor
Lance A Liotta
Nicole Simone
Michael Emmert-Buck
Emmanuel F Petricoin Iii
Original Assignee
Us Health
Lance A Liotta
Nicole Simone
Emmert Buck Michael
Emmanuel F Petricoin Iii
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Us Health, Lance A Liotta, Nicole Simone, Emmert Buck Michael, Emmanuel F Petricoin Iii filed Critical Us Health
Priority to AU33671/00A priority Critical patent/AU772680B2/en
Priority to US09/913,667 priority patent/US6969614B1/en
Priority to JP2000600101A priority patent/JP2002537561A/en
Priority to EP00911845A priority patent/EP1153300A2/en
Priority to CA002359649A priority patent/CA2359649A1/en
Publication of WO2000049410A2 publication Critical patent/WO2000049410A2/en
Publication of WO2000049410A3 publication Critical patent/WO2000049410A3/en
Publication of WO2000049410A9 publication Critical patent/WO2000049410A9/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • G01N2001/2833—Collecting samples on a sticky, tacky, adhesive surface
    • G01N2001/284—Collecting samples on a sticky, tacky, adhesive surface using local activation of adhesive, i.e. Laser Capture Microdissection
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2550/00—Electrophoretic profiling, e.g. for proteome analysis
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

The present invention describes devices and methods for performing protein analysis on laser capture microdissected cells, which permits proteomic analysis on cells of different populations. Particular disclosed examples are analysis of normal versus malignant cells, or a comparison of differential protein expression in cells that are progressing from normal to malignant. The protein content of the microdissected cells may be analyzed using techniques such as immunoassays, 1D and 2D gel electrophoresis characterization, Western blotting, liquid chromatography quadrapole ion trap electrospray (LCQ-MS), Matrix Assisted Laser Desorption Ionization/Time of Flight (MALDI/TOF), and Surface Enhanced Laser Desorption Ionization Spectroscopy (SELDI). In addition to permitting direct comparison of qualitative and quantitative protein content of tumor cells and normal cells from the same tissue sample, the methods also allow for investigation of protein characteristics of tumor cells, such as binding ability and amino acid sequence, and differential expression of proteins in particular cell populations in response to drug treatment. The present methods also provide, through the use of protein fingerprinting, a rapid and reliable way to identify the source tissue of a tumor metastasis.
PCT/US2000/004023 1999-02-16 2000-02-16 Lcm (laser capture microdissection) for cellular protein analysis WO2000049410A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AU33671/00A AU772680B2 (en) 1999-02-16 2000-02-16 LCM (Laser capture microdissection) for cellular protein analysis
US09/913,667 US6969614B1 (en) 1999-02-16 2000-02-16 Methods for the isolation and analysis of cellular protein content
JP2000600101A JP2002537561A (en) 1999-02-16 2000-02-16 Methods and apparatus for isolating and analyzing cellular protein components
EP00911845A EP1153300A2 (en) 1999-02-16 2000-02-16 Lcm (laser capture microdissection) for cellular protein analysis
CA002359649A CA2359649A1 (en) 1999-02-16 2000-02-16 Lcm (laser capture microdissection) for cellular protein analysis

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12028899P 1999-02-16 1999-02-16
US60/120,288 1999-02-16

Publications (3)

Publication Number Publication Date
WO2000049410A2 WO2000049410A2 (en) 2000-08-24
WO2000049410A3 true WO2000049410A3 (en) 2001-03-08
WO2000049410A9 WO2000049410A9 (en) 2001-08-30

Family

ID=22389357

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/004023 WO2000049410A2 (en) 1999-02-16 2000-02-16 Lcm (laser capture microdissection) for cellular protein analysis

Country Status (5)

Country Link
EP (1) EP1153300A2 (en)
JP (1) JP2002537561A (en)
AU (1) AU772680B2 (en)
CA (1) CA2359649A1 (en)
WO (1) WO2000049410A2 (en)

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Also Published As

Publication number Publication date
AU772680B2 (en) 2004-05-06
JP2002537561A (en) 2002-11-05
EP1153300A2 (en) 2001-11-14
AU3367100A (en) 2000-09-04
WO2000049410A2 (en) 2000-08-24
WO2000049410A9 (en) 2001-08-30
CA2359649A1 (en) 2000-08-24

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