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WO1991010910A3 - Process for enzymatically treating substrates - Google Patents

Process for enzymatically treating substrates Download PDF

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Publication number
WO1991010910A3
WO1991010910A3 PCT/EP1991/000086 EP9100086W WO9110910A3 WO 1991010910 A3 WO1991010910 A3 WO 1991010910A3 EP 9100086 W EP9100086 W EP 9100086W WO 9110910 A3 WO9110910 A3 WO 9110910A3
Authority
WO
WIPO (PCT)
Prior art keywords
fusion protein
supporting base
substrate
codes
brought
Prior art date
Application number
PCT/EP1991/000086
Other languages
German (de)
French (fr)
Other versions
WO1991010910A2 (en
Inventor
Rainer Rudolph
Erhard Kopetzki
Stephan Fischer
Adelbert Grossmann
Baerbel Hoell-Neugebauer
Original Assignee
Boehringer Mannheim Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE4001508A external-priority patent/DE4001508A1/en
Priority claimed from DE4002636A external-priority patent/DE4002636A1/en
Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh
Publication of WO1991010910A2 publication Critical patent/WO1991010910A2/en
Priority to FI914389A priority Critical patent/FI914389A0/en
Priority to NO913673A priority patent/NO913673D0/en
Priority to KR1019910701147A priority patent/KR920701822A/en
Publication of WO1991010910A3 publication Critical patent/WO1991010910A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G01N33/56988—HIV or HTLV
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/735—Fusion polypeptide containing domain for protein-protein interaction containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16111—Human Immunodeficiency Virus, HIV concerning HIV env
    • C12N2740/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16211—Human Immunodeficiency Virus, HIV concerning HIV gagpol
    • C12N2740/16222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Virology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • AIDS & HIV (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

In order to treat a substrate with enzymes, the substrate to be treated is brought into contact with a biocatalyzer obtained by combining a gene that codes for a biologically active substance and a fragment of DNA that codes for a binding peptide capable of interacting with a supporting base, to produce a fusion protein. The fusion protein is inserted in an appropriate vector, transformed in an appropriate organism, the organism is cultivated, its cells are dissolved and the lysate containing the fusion protein is brought into contact with a supporting base capable of binding with the binding peptide. The fusion protein is bound to the supporting base by intermolecular interaction, and the enzymatically treated substrate is thus finally obtained.
PCT/EP1991/000086 1990-01-19 1991-01-18 Process for enzymatically treating substrates WO1991010910A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
FI914389A FI914389A0 (en) 1990-01-19 1991-09-18 FOERFARANDE FOER ENZYMATISK BEHANDLING AV SUBSTRAT.
NO913673A NO913673D0 (en) 1990-01-19 1991-09-18 PROCEDURE FOR ENZYMATIC TREATMENT OF SUBSTRATES.
KR1019910701147A KR920701822A (en) 1990-01-19 1991-09-18 Method of enzymatic treatment of substrate

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DEP4001508.4 1990-01-19
DE4001508A DE4001508A1 (en) 1990-01-19 1990-01-19 Treating substrate with immobilised enzyme as fusion protein - with binding peptide specifically interacting with carrier, and new immunoassay using receptor-binding peptide fusion protein
DEP4002636.1 1990-01-30
DE4002636A DE4002636A1 (en) 1990-01-30 1990-01-30 EXPRESSION OF HIV1 AND 2 POLYPEPTIDES AND THEIR USE

Publications (2)

Publication Number Publication Date
WO1991010910A2 WO1991010910A2 (en) 1991-07-25
WO1991010910A3 true WO1991010910A3 (en) 1991-10-03

Family

ID=25889228

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1991/000086 WO1991010910A2 (en) 1990-01-19 1991-01-18 Process for enzymatically treating substrates

Country Status (6)

Country Link
EP (1) EP0464184A1 (en)
JP (1) JPH04503610A (en)
KR (1) KR920701822A (en)
AU (1) AU633686B2 (en)
CA (1) CA2047235A1 (en)
WO (1) WO1991010910A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6316004B1 (en) * 1993-06-22 2001-11-13 T. Tikhonenko Chimeric somatostatin containing protein and encoding DNA, plasmids of expression, method for preparing chimeric protein, strain-producers, immunogenic composition, method for increasing the productivity of farm animals

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0089626A2 (en) * 1982-03-19 1983-09-28 G.D. Searle & Co. Process for the preparation of polypeptides utilizing a charged amino acid polymer and exopeptidase
EP0282042A2 (en) * 1987-03-10 1988-09-14 F. Hoffmann-La Roche Ag Fusion proteins and their purification
EP0286239A1 (en) * 1987-03-10 1988-10-12 New England Biolabs, Inc. Production and purification of a protein fused to a binding protein
EP0289339A1 (en) * 1987-05-01 1988-11-02 Cambridge Bioscience Corporation Detection of antibodies to human immunodeficiency virus
EP0326100A2 (en) * 1988-01-29 1989-08-02 Abbott Laboratories Ion-capture assays and devices
EP0339389A1 (en) * 1988-04-25 1989-11-02 F. Hoffmann-La Roche Ag Test device
EP0348780A2 (en) * 1988-06-30 1990-01-03 Hoechst Aktiengesellschaft Process for producing proteins

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0089626A2 (en) * 1982-03-19 1983-09-28 G.D. Searle & Co. Process for the preparation of polypeptides utilizing a charged amino acid polymer and exopeptidase
EP0282042A2 (en) * 1987-03-10 1988-09-14 F. Hoffmann-La Roche Ag Fusion proteins and their purification
EP0286239A1 (en) * 1987-03-10 1988-10-12 New England Biolabs, Inc. Production and purification of a protein fused to a binding protein
EP0289339A1 (en) * 1987-05-01 1988-11-02 Cambridge Bioscience Corporation Detection of antibodies to human immunodeficiency virus
EP0326100A2 (en) * 1988-01-29 1989-08-02 Abbott Laboratories Ion-capture assays and devices
EP0339389A1 (en) * 1988-04-25 1989-11-02 F. Hoffmann-La Roche Ag Test device
EP0348780A2 (en) * 1988-06-30 1990-01-03 Hoechst Aktiengesellschaft Process for producing proteins

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Biotechnology, Band 7, Nr. 6, Juni 1989, (New York, US), E. Ong et al.: "Enzyme immobilization using the cellulose-binding domain of a cellulomonas fimi exoglucanase", Seiten 604-607 *

Also Published As

Publication number Publication date
CA2047235A1 (en) 1991-07-20
WO1991010910A2 (en) 1991-07-25
AU633686B2 (en) 1993-02-04
JPH04503610A (en) 1992-07-02
EP0464184A1 (en) 1992-01-08
KR920701822A (en) 1992-08-12
AU7072491A (en) 1991-08-05

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