US5514587A - DNA fragment encoding a hydrogen peroxide-generating NADH oxidase - Google Patents
DNA fragment encoding a hydrogen peroxide-generating NADH oxidase Download PDFInfo
- Publication number
- US5514587A US5514587A US08/220,677 US22067794A US5514587A US 5514587 A US5514587 A US 5514587A US 22067794 A US22067794 A US 22067794A US 5514587 A US5514587 A US 5514587A
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- ala
- gly
- leu
- val
- glu
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
Definitions
- the present invention relates to a DNA fragment encoding an enzyme that catalyzes the reaction in which NADH is oxidized by oxygen molecules to generate hydrogen peroxide (Japanese Laid-Open Publication 2-407889) and a method for preparing the enzyme with the use of a microorganism including a plasmid containing the fragment.
- the enzyme is useful for detection of a very small amount of substance in vivo with the use of the reaction generating NADH.
- Hydrogen peroxide-generating enzymes can be found in a culture of Streptococus mutans. However, it is difficult to obtain a stable culture condition in which water-generating enzymes can be obtained in a much higher level than the level of hydrogen peroxide-generating enzymes, which can also be found in the culture. Moreover, it is necessary to separate hydrogen peroxide generating enzymes from water-generating enzymes in the culture. Therefore, a method for preparing a large amount of hydrogen peroxide-generating NADH oxidases stably in a rational way, i.e., a method for preparing NADH oxidases with the use of gene recombinant techniques is desired.
- the conventional method for preparing NADH oxidases with a culture of Streptococus mutans is not suitable for obtaining a large amount of a hydrogen peroxide-generating NADH oxidase. Therefore, one of the objectives of the present invention is to provide a gene encoding a NADH oxidase, which can be used in a method for preparing a large amount of a hydrogen peroxide-generating NADH oxidase by gene recombinant techniques.
- the present invention provides a DNA fragment encoding the hydrogen peroxide-generating NADH oxidase.
- the present invention also provides a DNA fragment that encodes a protein containing a NADH binding site of the NADH oxidase having the following amino acid sequence:
- the present invention provides a DNA fragment of following base sequence that encodes a protein containing a NADH binding site of the NADH oxidase:
- the present invention also provides a DNA fragment encoding a protein containing a FAD binding site of the NADH oxidase having the following amino acid sequence:
- the present invention provides a DNA fragment of the following base sequence which encodes a protein containing a FAD binding site of the NADH oxidase:
- the present invention also provides a DNA fragment of following base sequence which encodes the NADH oxidase:
- the present invention also provides a DNA fragment encoding the NADH oxidase having the following amino acid sequence:
- upstream region of the DNA fragment of the present invention may be a non-translational region having the following base sequence:
- the gene encoding the NADH oxidase of the present invention may be cloned by standard methods. For example, first, genomic DNA is prepared from a microorganism having an ability to produce a hydroxy peroxide-generating NADH oxidase. The prepared genomic DNA is digested with an appropriate restriction enzyme to obtain DNA fragments. A vector is also digested with the appropriate restriction enzymes. One of the obtained DNA fragments and the digested vector are ligated by the use of a T4 DNA ligase to obtain recombinant DNA.
- the obtained recombinant DNAs are further treated with appropriate restriction enzymes. Then each of the treated DNA fragments is ligated into a cloning vector and the obtained recombinant vector is introduced into a host microorganism to prepare a transformant.
- the transformants may be screened for desired transformants by a standard method in order to clone a gene encoding the NADH oxidase.
- Streptococus mutans NCIB11723 is cultured in a medium containing appropriate amounts of carbon, nitrogen, inorganic salts, and other nutrient sources and the resulting culture is centrifuged to collect the cells.
- preferable mediums include a brain-heart infusion medium and the like.
- the temperature for culture is in the range from 20° to 40° C., preferably, from 30° to 37° C.
- the culture starts with pH6 to 8, preferably about 7.
- the culture continues until about the middle of the log growth phase by a stand culture.
- the DNA can be prepared from lytic cells.
- Methods for lysing cells include cell treatments with cell wall-lytic enzymes, such as a lysozyme and an ⁇ -glucanase, and optionally together with other enzymes or surfactant, such as sodium lauryl sulfate.
- cell wall-lytic enzymes such as a lysozyme and an ⁇ -glucanase
- surfactant such as sodium lauryl sulfate
- DNA can be isolated and purified from lytic cells according to standard techniques, such as appropriate combination of phenol extraction, protein removing, protease treatment, ribonuclease treatment, alcohol precipitation, centrifugation, and the like.
- Methods for cleaving DNAs include ultrasonication, restriction enzyme treatment and the like. After being cleaved, a base sequence of the terminal of a DNA fragment can be altered by the treatment with modifying enzymes, such as phosphatases and DNA polymerases.
- cleaved DNAs can be gel electrophoresed and subjected to Southern hybridization (Southern, E. M., J. Mol. Biol., 98, 503 (1975)) with the use of synthetic probes based on amino acid sequence of the desired protein. Positive DNA fragment(s) can be extracted from a gel to obtain appropriate length of fragment(s).
- vectors those derived from phages or plasmids, which can autonomically propagate in a host cell can be used.
- ⁇ phages, M13 phages, pBR322, pUC118, and pUC119 can be used for Escherichia coli (E. coli) host cell.
- Method for ligating a DNA fragment and a vector fragment can use any methods using known DNA ligase and the like.
- a DNA fragment and a vector fragment can be ligated in vitro with the action of an appropriate DNA ligase to prepare recombinant DNA.
- any microorganism in which recombinant DNAs can be stably maintained and autonomically propagate, and be expressed, can be used.
- Methods for introducing recombinant DNAs into host microorganism include known methods, for example, when a host microorganism is E. coli, a calcium method can be used (Lederberg. E. M., & Cohen. S. N., J. Bacteriol., 119, 1072 (1974)).
- ⁇ phage particles are first formed by an in vitro packaging method (Horn, B., Methods in Enzymol., 68, 299 (1979)). Then the particles are added to a culture suspension of E. coli to obtain transduced phages with an ability to produce the NADH oxidase.
- Methods for selecting transformed microorganisms containing recombinant DNA include standard methods, such as colony hybridization method for obtaining positive clones.
- the obtained transformants are cultured in a liquid at 37° C. and plasmids in the transformants can be obtained by known methods, such as an alkaline extraction method (Birnboim, H. C. & Doly, J., Nucleic Acids Res., 7, 1513 (1979)).
- the sequence of inserts in the plasmids can be determined by a dideoxy method (Sanger, F., Nickelen, S., & Colusion, A. R., Proc. Natl. Acad. Sci. 74, 5493 (1977)).
- a large amount of the desired hydrogen peroxide-generating NADH oxidase can be obtained by culturing the selected transformants.
- Streptococus mutans NCIB11723 (The National Collection of Industrial and Marin Bacteria, 23 Street, Machar Drive, Aberdeen, U.K.) was cultured in a brain-heart infusion liquid medium at 37° C. for 6 hours and then centrifuged to collect the cells. The obtained cells were washed and treated with a lysozyme, N-acetylmuramidase (2000 U/ml). Then genomic DNA was isolated by a method of Saito-Miura (Saito, H. & Miura, K., Biochem. Biophys. Acta, 72, 619 (1963)). The isolated genomic DNA was dissolved in a Tris-Hcl/EDTA buffer and partially digested with Sau3A1.
- the digested fragments were separated by a 10 to 40% sucrose density-gradient centrifugation to obtain DNA fragments with 9 to 23 kb.
- the genomic DNA fragments were ligated with arms of ⁇ EMBL3 phage (Stratagene Cloning Systems, 11099 North Torry, Pines Road, La Jolla Calif. 92037, U.S.A.) by the use of T4 DNA ligase.
- the ligated fragments were packed in phage particles by using in vitro packaging kit (Stratagene Cloning Systems, 11099 North Torry, Pines Road, La Jolla Calif. 92037, U.S.A.).
- E. coli P2392 (Stratagene Cloning Systems, 11099 North Torry, Pines Road, La Jolla Calif. 92037, U.S.A.) was infected with the phage particles.
- the infected plaques of E. coli were screened by a plaque hybridization method with the use of synthetic probes based on the N terminal amino acid sequence of hydrogen peroxide-generating NADH oxidase. A positive clone hybridizing with the synthetic probes was selected.
- the positive clone obtained in Example 1 was also reacted with an antibody directed to the hydrogen peroxide-generating NADH oxidase.
- DNA was extracted from the positive clone and digested with BamH1 to obtain a DNA fragment with 4 kbp.
- the DNA fragment and plasmid pMW (a plasmid with an unique BamH1 site and an ampicillin resistance gene; Nippon-Gene, 1-29, Tonyacho, Toyama 930, Japan) digested with BamH1 were mixed and ligated by adding a T4 DNA ligase in the mixture. The mixture was used to introduce the recombinant plasmid into E.
- the positive clone was cultured and centrifuged to collect the cells. After the cells were washed, the plasmid was extracted with an alkaline method. The obtained plasmid was designated as pHS19.
- Deletion variants including inserts with a variety of sizes necessary for the determination of base sequences were prepared with the use of kilo sequencing deletion kit (Takara Shuzo Co., Ltd., Shijo-Higashinotoin, Shimogyo-ku, Kyoto 600-91, Japan).
- a DNA base sequence was determined by a dideoxy nucleotide chain termination method (Messing, J. & Vieira, J., Gene, 19, 269 (1982).
- the obtained base sequence is given in SEQ ID NO: 1.
- 1-278 is a non-translational region containing promoter region
- 279-1810 is a structural DNA.
- An amino acid sequence deduced from the structural DNA is given in SEQ ID NO: 2.
- the obtained amino acid sequence was shown a 54.6% homology with the NADH oxidase encoding DNA fragment derived from Amphibacillus xylanus(GENETYX: Amino Acid Homology Data).
- binding sites for NADH, the substrate of the NADH oxidase and FAD contain amino acid sequence -GXXXXG-, wherein G is glycine and X may be any amino acid residue (Ho-Jin PARK, et. al., Eur. J. Biochem., 205, 875-879(1992)).
- the amino acid sequence given in SEQ ID NO: 2, obtained in above Example 3 was analyzed and find two sites having the sequences corresponding to -GXXXXG-. Therefore, we assumed one of which is a NADH binding site and the another is a FAD binding site.
- a NADH binding site of the NADH oxidase derived from Amphibacilius xylanus contains an amino acid sequence of -GGGPAG- (Gly Gly Gly Pro Ala Gly), and a FAD binding site contains -GGGNSG- (Gly Gly Gly Asn Ser Gly)(Nimura, Y. et. al.: Annual Meeting of Japanese Society of Agricultural Chemistry (1992)).
- 215-220 of SEQ ID NO: 2 is same as the sequence of the NADH binding site, and 354-359 is same as that of FAD binding site. Therefore, it is concluded that in the SEQ ID NO. 2, the regions of 215-220 and 354-359 are each corresponding to a NADH binding site and a FAD binding site of the NADH oxidase.
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Abstract
A gene encoding a hydrogen peroxide-generating NADH oxidase and a method for preparing a large amount of the NADH oxidase with the use of the gene and gene recombinant techniques are disclosed.
Description
1. Field of the Invention
The present invention relates to a DNA fragment encoding an enzyme that catalyzes the reaction in which NADH is oxidized by oxygen molecules to generate hydrogen peroxide (Japanese Laid-Open Publication 2-407889) and a method for preparing the enzyme with the use of a microorganism including a plasmid containing the fragment. The enzyme is useful for detection of a very small amount of substance in vivo with the use of the reaction generating NADH.
2. Description of the Prior Art
NADH oxidases are divided into two groups, one group which generates hydrogen peroxide (hydrogen peroxide-generating enzymes) and the other group which generates water (water-generating enzymes) by the reaction with oxygen molecules.
Hydrogen peroxide-generating enzymes can be found in a culture of Streptococus mutans. However, it is difficult to obtain a stable culture condition in which water-generating enzymes can be obtained in a much higher level than the level of hydrogen peroxide-generating enzymes, which can also be found in the culture. Moreover, it is necessary to separate hydrogen peroxide generating enzymes from water-generating enzymes in the culture. Therefore, a method for preparing a large amount of hydrogen peroxide-generating NADH oxidases stably in a rational way, i.e., a method for preparing NADH oxidases with the use of gene recombinant techniques is desired.
The conventional method for preparing NADH oxidases with a culture of Streptococus mutans is not suitable for obtaining a large amount of a hydrogen peroxide-generating NADH oxidase. Therefore, one of the objectives of the present invention is to provide a gene encoding a NADH oxidase, which can be used in a method for preparing a large amount of a hydrogen peroxide-generating NADH oxidase by gene recombinant techniques.
As the result of our researches to develop a method for preparing a hydrogen peroxide-generating NADH oxidase, we have eventually cloned a gene encoding hydrogen peroxide-generating NADH oxidase derived from Streptococus mutans and sequenced the gene.
The present invention, provides a DNA fragment encoding the hydrogen peroxide-generating NADH oxidase.
The present invention also provides a DNA fragment that encodes a protein containing a NADH binding site of the NADH oxidase having the following amino acid sequence:
__________________________________________________________________________
Val
Leu Val Ile
Gly
Gly
Gly
Pro
Ala
Gly
Asn
Ser
Ala
Ala Ile Tyr
Ala
Ala
Arg
Lys
Gly
Val
Lys
Thr
__________________________________________________________________________
The present invention provides a DNA fragment of following base sequence that encodes a protein containing a NADH binding site of the NADH oxidase:
__________________________________________________________________________
GTCCTTGTTA
TTGGTGGGGG
TCCTGCTGGT
AATAGCGCGG
CTATCTATGC
TGCAAGAAAG
GGAGTTAAAA
CA
__________________________________________________________________________
The present invention also provides a DNA fragment encoding a protein containing a FAD binding site of the NADH oxidase having the following amino acid sequence:
__________________________________________________________________________
Val
Ala Val Ile
Gly
Gly
Gly
Asn
Ser
Gly
Leu
Glu
Ala
Ala Ile Asp
Leu
Ala
Gly
Leu
Ala
Ser
His
Val
Tyr
__________________________________________________________________________
The present invention provides a DNA fragment of the following base sequence which encodes a protein containing a FAD binding site of the NADH oxidase:
__________________________________________________________________________
GTCGCTGTCA
TTGGCGGTGG
AAACTCAGGT
TTAGAAGCAG
TCATTGATTT
GGCTGGGTTA
GCTAGCCATG
TCTAT
__________________________________________________________________________
The present invention also provides a DNA fragment of following base sequence which encodes the NADH oxidase:
__________________________________________________________________________
AT GGCATTAGAC
GCAGAAATCA
AAGAGCAGTT
AGGACAGTAT
CTTCAATTAC
TTGAGTGTGA
GATTGTTTTA
CAAGCTCAAT
TAAAAGACGA
TGCTAATTCT
CAAAAAGTTA
AGGAATTTCT
CCAAGAAATC
GTTGCAATGT
CTCCTATGAT
TTCTTTAGAC
GAAAAGGAAC
TTCCGCGAAC
ACCTAGTTTT
CGCATAGCTA
AAAAGGGGCA
AGAATCTGGT
GTTGAATTTG
CTGGCTTACC
CCTTGGTCAC
GAATTTTACT
TCGTTTATCT
TGGCTCTGTT
ACAGGTTTCA
GGGCGTCCGC
TAAGGTAGAG
ACTGATATTG
TCAAACGCAT
TCAAGCTGTT
GATGAACCTA
TGCATTTTGA
AACCTATGTT
AGTTTGACTT
GTCATAATTG
TCCAGATGTT
GTTCAGGCTT
TCAATATCAT
GTCAGTTGTT
AATCCCAACA
TTTCACATAC
AATGGTGGAA
GGTGGCATGT
TTAAAGATGA
AATTGAAGCT
AAGGGAATTA
TGTCTGTGCC
AACTGTCTAT
AAAGATGGAA
CAGAATTTAC
CTCAGGGCGT
GCTAGCATAG
AGCAATTACT
AGACTTGATA
GCAGGTCCTC
TTAAAGAAGA
TGCTTTTGAT
GATAAAGGTG
TTTTTGATGT
CCTTGTTATT
GGTGGGGGTC
CTGCTGGTAA
TAGCGCGGCT
ATCTATGCTG
CAAGAAAGGG
AGTTAAAACA
GGACTTTTAG
CTGAAACCAT
GGGTGGTCAA
GTTATGGAAA
CCGTGGGTAT
TGAAAATATG
ATCGGTACCC
CATATGTTGA
AGGACCCCAA
TTAATGGCTC
AGGTGGAAGA
GCATACCAAG
TCTTATTCTG
TTGACATCAT
GAAGGCACCG
CGTGCTAAGT
CTATTCAAAA
GACAGACTTG
GTTGAAGTTG
AACTTGATAA
TGGAGCTCAT
TTGAAAGCAA
AGACAGCTGT
TTTGGCCTTA
GGTGCCAAGT
GGCGTAAAAT
CAATGTACCA
GGAGAAAAAG
AATTCTTTAA
TAAAGGTGTT
ACTTACTGTC
CGCACTGTGA
TGGTCCTCTT
TTCACAGACA
AAAAAGTCGC
TGTCATTGGC
GGTGGAAACT
CAGGTTTAGA
AGCAGCTATT
GATTTGGCTG
GGTTAGCTAG
CCATGTCTAT
ATTTTAGAAT
TTTTACCTGA
GTTAAAAGCT
GATAAGATCT
TACAAGATCG
TGCGGAAGCT
CTTGATAATA
TTACCATTCT
AACTAATGTT
GCGACTAAAG
AAATTATTGG
CAATGACCAC
GTAGAAGGTC
TTCGTTACAG
TGATCGTACG
ACCAATGAAG
AGTACTTGCT
TGATTTAGAA
GGTGTTTTTG
TTCAAATTGG
ATTGGTACCT
AGTACTGACT
GGTTAAAGGA
TAGTGGACTA
GCACTCAATG
AAAAAGGTGA
AATCATTGTT
GCTAAAGATG
GCGCAACTAA
TATTCCTGCT
ATTTTTGCAG
CTGGTGATTG
CACAGATAGT
GCCTACAAAC
AAATTATCAT
TTCCATGGGT
TCTGGAGCTA
CTGCGGCTTT
AGGTGCCTTT
GATTATTTGA
TTAGAAATT//
__________________________________________________________________________
The present invention also provides a DNA fragment encoding the NADH oxidase having the following amino acid sequence:
__________________________________________________________________________
Met
Ala
Leu
Asp
Ala
Glu
Ile
Lys
Glu
Gln
Leu
Gly
Gln
Tyr
Leu
15
Gln
Leu
Leu
Glu
Cys
Glu
Ile
Val
Leu
Gln
Ala
Gln
Leu
Lys
Asp
30
Asp
Ala
Asn
Ser
Gln
Lys
Val
Lys
Glu
Phe
Leu
Gln
Glu
Ile
Val
45
Ala
Met
Ser
Pro
Met
Ile
Ser
Leu
Asp
Glu
Lys
Glu
Leu
Pro
Arg
60
Thr
Pro
Ser
Phe
Arg
Ile
Ala
Lys
Lys
Gly
Gln
Glu
Ser
Gly
Val
75
Glu
Phe
Ala
Gly
Leu
Pro
Leu
Gly
His
Glu
Phe
Tyr
Phe
Val
Tyr
90
Leu
Gly
Ser
Val
Thr
Gly
Phe
Arg
Ala
Ser
Ala
Lys
Val
Glu
Thr
105
Asp
Ile
Val
Lys
Arg
Ile
Gln
Ala
Val
Asp
Glu
Pro
Met
His
Phe
120
Glu
Thr
Tyr
Val
Ser
Leu
Thr
Cys
His
Asn
Cys
Pro
Asp
Val
Val
135
Gln
Ala
Phe
Asn
Ile
Met
Ser
Val
Val
Asn
Pro
Asn
Ile
Ser
His
150
Thr
Met
Val
Glu
Gly
Gly
Met
Phe
Lys
ASp
Glu
Ile
Glu
Ala
Lys
165
Gly
Ile
Met
Ser
Val
Pro
Thr
Val
Tyr
Lys
Asp
Gly
Thr
Glu
Phe
180
Thr
Ser
Gly
Arg
Ala
Ser
Ile
Glu
Gln
Leu
Leu
Asp
Leu
Ile
Ala
195
Gly
Pro
Leu
Lys
Glu
Asp
Ala
Phe
Asp
Asp
Lys
Gly
Val
Phe
Asp
210
Val
Leu
Val
Ile
Gly
Gly
Gly
Pro
Ala
Gly
Asn
Ser
Ala
Ala
Ile
225
Tyr
Ala
Ala
Arg
Lys
Gly
Val
Lys
Thr
Gly
Leu
Leu
Ala
Glu
Thr
240
Met
Gly
Gly
Gln
Val
Met
Glu
Thr
Val
Gly
Ile
Glu
Asn
Met
Ile
255
Gly
Thr
Pro
Tyr
Val
Glu
Gly
Pro
Gln
Leu
Met
Ala
Gln
Val
Glu
270
Glu
His
Thr
Lys
Ser
Tyr
Ser
Val
Asp
Ile
Met
Lys
Ala
Pro
Arg
285
Ala
Lys
Ser
Ile
Gln
Lys
Thr
Asp
Leu
Val
Glu
Val
Glu
Leu
Asp
300
Asn
Gly
Ala
His
Leu
Lys
Ala
Lys
Thr
Ala
Val
Lue
Ala
Lue
Gly
315
Ala
Lys
Trp
Arg
Lys
Ile
Asn
Val
Pro
Gly
Glu
Lys
Glu
Phe
Phe
330
Asn
Lys
Gly
Val
Thr
Tyr
Cys
Pro
His
Cys
Asp
Gly
Pro
Leu
Phe
345
Thr
Asp
Lys
Lys
Val
Ala
Val
Ile
Gly
Gly
Gly
Asn
Ser
Gly
Leu
360
Glu
Ala
Ala
Ile
Asp
Leu
Ala
Gly
Leu
Ala
Ser
His
Val
Tyr
Ile
375
Leu
Glu
Phe
Leu
Pro
Glu
Leu
Lys
Ala
Asp
Lys
Ile
Leu
Gln
Asp
390
Arg
Ala
Glu
Ala
Lue
Asp
Asn
Ile
Thr
Ile
Leu
Thr
Asn
Val
Ala
405
Thr
Lys
Glu
Ile
Ile
Gly
Asn
Asp
His
Val
Glu
Gly
Leu
Arg
Tyr
420
Ser
Asp
Arg
Thr
Thr
Asn
Glu
Glu
Tyr
Leu
Leu
Asp
Leu
Glu
Gly
435
Val
Phe
Val
Gln
Ile
Gly
Leu
Val
Pro
Ser
Thr
Asp
Trp
Leu
Lys
450
Asp
Ser
Gly
Leu
Ala
Leu
Asn
Glu
Lys
Gly
Glu
Ile
Ile
Val
Ala
465
Lys
Asp
Gly
Ala
Thr
Asn
Ile
Pro
Ala
Ile
Phe
Ala
Ala
Gly
Asp
480
Cys
Thr
Asp
Ser
Ala
Tyr
Lys
Gln
Ile
Ile
Ile
Ser
Met
Gly
Ser
495
Lys
Ala
Thr
Ala
Ala
Leu
Gly
Ala
Phe
Asp
Tyr
Leu
Ile
Arg
Asn
510
__________________________________________________________________________
In the upstream region of the DNA fragment of the present invention may be a non-translational region having the following base sequence:
__________________________________________________________________________
GGATCCCTTC
TCATGTTCTC
TCACAAGGAT
TTGAGGTTTT
AGGTGAAGAT
GGTTTAGCAC
60
AACGTGGAAC
CTTTATTGTA
GATCCGGATG
GTATCATTCA
AATGATGGAA
GTCAATGCAG
120
ATGGTATTGG
TCGTGATGCT
AGTACCTTGA
TTGATAAAGT
TCGTGCAGCT
CAATCTATTC
180
GCCAACATCC
AGGAGAAGTT
TGCCCTGCCA
AATGGAAAGA
GGGAGCTGAA
ACTTTAAAAC
240
CAAGTTTGGT
ACTTGTCGGT
AAAATTTAAG
GAGAAACT
__________________________________________________________________________
The gene encoding the NADH oxidase of the present invention may be cloned by standard methods. For example, first, genomic DNA is prepared from a microorganism having an ability to produce a hydroxy peroxide-generating NADH oxidase. The prepared genomic DNA is digested with an appropriate restriction enzyme to obtain DNA fragments. A vector is also digested with the appropriate restriction enzymes. One of the obtained DNA fragments and the digested vector are ligated by the use of a T4 DNA ligase to obtain recombinant DNA.
The obtained recombinant DNAs are further treated with appropriate restriction enzymes. Then each of the treated DNA fragments is ligated into a cloning vector and the obtained recombinant vector is introduced into a host microorganism to prepare a transformant. The transformants may be screened for desired transformants by a standard method in order to clone a gene encoding the NADH oxidase.
In more detail, Streptococus mutans NCIB11723 is cultured in a medium containing appropriate amounts of carbon, nitrogen, inorganic salts, and other nutrient sources and the resulting culture is centrifuged to collect the cells. Examples of preferable mediums include a brain-heart infusion medium and the like. The temperature for culture is in the range from 20° to 40° C., preferably, from 30° to 37° C. The culture starts with pH6 to 8, preferably about 7. The culture continues until about the middle of the log growth phase by a stand culture. The DNA can be prepared from lytic cells.
Methods for lysing cells include cell treatments with cell wall-lytic enzymes, such as a lysozyme and an α-glucanase, and optionally together with other enzymes or surfactant, such as sodium lauryl sulfate.
DNA can be isolated and purified from lytic cells according to standard techniques, such as appropriate combination of phenol extraction, protein removing, protease treatment, ribonuclease treatment, alcohol precipitation, centrifugation, and the like.
Methods for cleaving DNAs include ultrasonication, restriction enzyme treatment and the like. After being cleaved, a base sequence of the terminal of a DNA fragment can be altered by the treatment with modifying enzymes, such as phosphatases and DNA polymerases.
To select DNA fragments having a base sequence encoding the desired protein, cleaved DNAs can be gel electrophoresed and subjected to Southern hybridization (Southern, E. M., J. Mol. Biol., 98, 503 (1975)) with the use of synthetic probes based on amino acid sequence of the desired protein. Positive DNA fragment(s) can be extracted from a gel to obtain appropriate length of fragment(s).
As for vectors, those derived from phages or plasmids, which can autonomically propagate in a host cell can be used. For example, λ phages, M13 phages, pBR322, pUC118, and pUC119 can be used for Escherichia coli (E. coli) host cell.
Method for ligating a DNA fragment and a vector fragment can use any methods using known DNA ligase and the like. For example, a DNA fragment and a vector fragment can be ligated in vitro with the action of an appropriate DNA ligase to prepare recombinant DNA.
As for host cell, any microorganism in which recombinant DNAs can be stably maintained and autonomically propagate, and be expressed, can be used.
Methods for introducing recombinant DNAs into host microorganism include known methods, for example, when a host microorganism is E. coli, a calcium method can be used (Lederberg. E. M., & Cohen. S. N., J. Bacteriol., 119, 1072 (1974)).
When the vector is derived from λ phage, λ phage particles are first formed by an in vitro packaging method (Horn, B., Methods in Enzymol., 68, 299 (1979)). Then the particles are added to a culture suspension of E. coli to obtain transduced phages with an ability to produce the NADH oxidase.
Methods for selecting transformed microorganisms containing recombinant DNA include standard methods, such as colony hybridization method for obtaining positive clones. The obtained transformants are cultured in a liquid at 37° C. and plasmids in the transformants can be obtained by known methods, such as an alkaline extraction method (Birnboim, H. C. & Doly, J., Nucleic Acids Res., 7, 1513 (1979)). The sequence of inserts in the plasmids can be determined by a dideoxy method (Sanger, F., Nickelen, S., & Colusion, A. R., Proc. Natl. Acad. Sci. 74, 5493 (1977)). A large amount of the desired hydrogen peroxide-generating NADH oxidase can be obtained by culturing the selected transformants.
Streptococus mutans NCIB11723 (The National Collection of Industrial and Marin Bacteria, 23 Street, Machar Drive, Aberdeen, U.K.) was cultured in a brain-heart infusion liquid medium at 37° C. for 6 hours and then centrifuged to collect the cells. The obtained cells were washed and treated with a lysozyme, N-acetylmuramidase (2000 U/ml). Then genomic DNA was isolated by a method of Saito-Miura (Saito, H. & Miura, K., Biochem. Biophys. Acta, 72, 619 (1963)). The isolated genomic DNA was dissolved in a Tris-Hcl/EDTA buffer and partially digested with Sau3A1. The digested fragments were separated by a 10 to 40% sucrose density-gradient centrifugation to obtain DNA fragments with 9 to 23 kb. The genomic DNA fragments were ligated with arms of λEMBL3 phage (Stratagene Cloning Systems, 11099 North Torry, Pines Road, La Jolla Calif. 92037, U.S.A.) by the use of T4 DNA ligase. The ligated fragments were packed in phage particles by using in vitro packaging kit (Stratagene Cloning Systems, 11099 North Torry, Pines Road, La Jolla Calif. 92037, U.S.A.). E. coli P2392 (Stratagene Cloning Systems, 11099 North Torry, Pines Road, La Jolla Calif. 92037, U.S.A.) was infected with the phage particles.
The infected plaques of E. coli were screened by a plaque hybridization method with the use of synthetic probes based on the N terminal amino acid sequence of hydrogen peroxide-generating NADH oxidase. A positive clone hybridizing with the synthetic probes was selected.
The positive clone obtained in Example 1 was also reacted with an antibody directed to the hydrogen peroxide-generating NADH oxidase. DNA was extracted from the positive clone and digested with BamH1 to obtain a DNA fragment with 4 kbp. The DNA fragment and plasmid pMW (a plasmid with an unique BamH1 site and an ampicillin resistance gene; Nippon-Gene, 1-29, Tonyacho, Toyama 930, Japan) digested with BamH1 were mixed and ligated by adding a T4 DNA ligase in the mixture. The mixture was used to introduce the recombinant plasmid into E. coli JM109 (Takara Shuzo Co., LTD., Shijo-Higashinotoin, Shimogyo-ku, Kyoto 600-91, Japan). The clone that reacted with the above-mentioned antibody was selected from the transformants.
The positive clone was cultured and centrifuged to collect the cells. After the cells were washed, the plasmid was extracted with an alkaline method. The obtained plasmid was designated as pHS19.
The plasmid PHS19 obtained in Example 2 was digested with EcoR1 to obtain DNA fragments with 1 kbp and 3 kbp. The DNA fragments were ligated with plasmid PUC118 or PUC119 and used for cloning of a gene encoding hydrogen peroxide-generating NADH oxidase. A transformant including the 1 kbp DNA fragment and a transformant including the 3 kbp DNA fragment were obtained. Plasmids were extracted from both of the transformants. Deletion variants including inserts with a variety of sizes necessary for the determination of base sequences were prepared with the use of kilo sequencing deletion kit (Takara Shuzo Co., Ltd., Shijo-Higashinotoin, Shimogyo-ku, Kyoto 600-91, Japan). A DNA base sequence was determined by a dideoxy nucleotide chain termination method (Messing, J. & Vieira, J., Gene, 19, 269 (1982).
The obtained base sequence is given in SEQ ID NO: 1. In this base sequence, 1-278 is a non-translational region containing promoter region, and 279-1810 is a structural DNA. An amino acid sequence deduced from the structural DNA is given in SEQ ID NO: 2. The obtained amino acid sequence was shown a 54.6% homology with the NADH oxidase encoding DNA fragment derived from Amphibacillus xylanus(GENETYX: Amino Acid Homology Data).
In an amino acid sequence of a NADH oxidase, it is presumed that binding sites for NADH, the substrate of the NADH oxidase and FAD (flavin coenzyme) contain amino acid sequence -GXXXXG-, wherein G is glycine and X may be any amino acid residue (Ho-Jin PARK, et. al., Eur. J. Biochem., 205, 875-879(1992)). The amino acid sequence given in SEQ ID NO: 2, obtained in above Example 3 was analyzed and find two sites having the sequences corresponding to -GXXXXG-. Therefore, we assumed one of which is a NADH binding site and the another is a FAD binding site.
A NADH binding site of the NADH oxidase derived from Amphibacilius xylanus contains an amino acid sequence of -GGGPAG- (Gly Gly Gly Pro Ala Gly), and a FAD binding site contains -GGGNSG- (Gly Gly Gly Asn Ser Gly)(Nimura, Y. et. al.: Annual Meeting of Japanese Society of Agricultural Chemistry (1992)).
Compared with both of the NADH and FAD binding sites of the NADH oxidase of Amphibacillus xylanus, 215-220 of SEQ ID NO: 2 is same as the sequence of the NADH binding site, and 354-359 is same as that of FAD binding site. Therefore, it is concluded that in the SEQ ID NO. 2, the regions of 215-220 and 354-359 are each corresponding to a NADH binding site and a FAD binding site of the NADH oxidase.
Accordingly, it is concluded that 921-938 of SEQ ID NO:1 is the region encoding the NADH binding site and 1338-1355 is that encoding the FAD binding site.
__________________________________________________________________________ SEQUENCE LISTING (1) GENERAL INFORMATION: (iii) NUMBER OF SEQUENCES: 2 (2) INFORMATION FOR SEQ ID NO:1: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 1809 base pairs (B) TYPE: nucleic acid (C) STRANDEDNESS: doubule (D) TOPOLOGY: linear (ii) MOLECULE TYPE: genomic DNA (iii) HYPOTHETICAL: No (iv) ANTI-SENSE: No (v) ORIGINAL SOURCE: (B) STRAIN:Streptococcus mutans NCIB11723 (vi) SEQUENCE DESCRIPTION: SEQ ID NO: 1: GGATCCCTTCTCATGTTCTCTCACAAGGATTTGAGGTTTTAGGTGAAGATGGTTTAGCAC60 AACGTGGAACCTTTATTGTAGATCCGGATGGTATCATTCAAATGATGGAAGTCAATGCAG120 ATGGTATTGGTCGTGATGCTAGTACCTTGATTGATAAAGTTCGTGCAGCTCAATCTATTC180 GCCAACATCCAGGAGAAGTTTGCCCTGCCAAATGGAAAGAGGGAGCTGAAACTTTAAAAC240 CAAGTTTGGTACTTGTCGGTAAAATTTAAGGAGAAACTATGGCATTAGACGCAGAAATCA300 AAGAGCAGTTAGGACAGTATCTTCAATTACTTGAGTGTGAGATTGTTTTACAAGCTCAAT360 TAAAAGACGATGCTAATTCTCAAAAAGTTAAGGAATTTCTCCAAGAAATCGTTGCAATGT420 CTCCTATGATTTCTTTAGACGAAAAGGAACTTCCGCGAACACCTAGTTTTCGCATAGCTA480 AAAAGGGGCAAGAATCTGGTGTTGAATTTGCTGGCTTACCCCTTGGTCACGAATTTTACT540 TCGTTTATCTTGGCTCTGTTACAGGTTTCAGGGCGTCCGCTAAGGTAGAGACTGATATTG600 TCAAACGCATTCAAGCTGTTGATGAACCTATGCATTTTGAAACCTATGTTAGTTTGACTT660 GTCATAATTGTCCAGATGTTGTTCAGGCTTTCAATATCATGTCAGTTGTTAATCCCAACA720 TTTCACATACAATGGTGGAAGGTGGCATGTTTAAAGATGAAATTGAAGCTAAGGGAATTA780 TGTCTGTGCCAACTGTCTATAAAGATGGAACAGAATTTACCTCAGGGCGTGCTAGCATAG840 AGCAATTACTAGACTTGATAGCAGGTCCTCTTAAAGAAGATGCTTTTGATGATAAAGGTG900 TTTTTGATGTCCTTGTTATTGGTGGGGGTCCTGCTGGTAATAGCGCGGCTATCTATGCTG960 CAAGAAAGGGAGTTAAAACAGGACTTTTAGCTGAAACCATGGGTGGTCAAGTTATGGAAA1020 CCGTGGGTATTGAAAATATGATCGGTACCCCATATGTTGAAGGACCCCAATTAATGGCTC1080 AGGTGGAAGAGCATACCAAGTCTTATTCTGTTGACATCATGAAGGCACCGCGTGCTAAGT1140 CTATTCAAAAGACAGACTTGGTTGAAGTTGAACTTGATAATGGAGCTCATTTGAAAGCAA1200 AGACAGCTGTTTTGGCCTTAGGTGCCAAGTGGCGTAAAATCAATGTACCAGGAGAAAAAG1260 AATTCTTTAATAAAGGTGTTACTTACTGTCCGCACTGTGATGGTCCTCTTTTCACAGACA1320 AAAAAGTCGCTGTCATTGGCGGTGGAAACTCAGGTTTAGAAGCAGCTATTGATTTGGCTG1380 GGTTAGCTAGCCATGTCTATATTTTAGAATTTTTACCTGAGTTAAAAGCTGATAAGATCT1440 TACAAGATCGTGCGGAAGCTCTTGATAATATTACCATTCTAACTAATGTTGCGACTAAAG1500 AAATTATTGGCAATGACCACGTAGAAGGTCTTCGTTACAGTGATCGTACGACCAATGAAG1560 AGTACTTGCTTGATTTAGAAGGTGTTTTTGTTCAAATTGGATTGGTACCTAGTACTGACT1620 GGTTAAAGGATAGTGGACTAGCACTCAATGAAAAAGGTGAAATCATTGTTGCTAAAGATG1680 GCGCAACTAATATTCCTGCTATTTTTGCAGCTGGTGATTGCACAGATAGTGCCTACAAAC1740 AAATTATCATTTCCATGGGTTCTGGAGCTACTGCGGCTTTAGGTGCCTTTGATTATTTGA1800 TTAGAAATT1809 (2) INFORMATION FOR SEQ ID NO:2: (i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 510 amino acids (B) TYPE: amino acids (D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein (iii) HYPOTHETICAL: No (iv) ANTI-SENSE: No (v) ORIGINAL SOURCE: (B) STRAIN:Streptococcus mutans NCIB11723 (vi) SEQUENCE DESCRIPTION: SEQ ID NO: 2: MetAlaLeuAspAlaGluIleLysGluGlnLeuGlyGlnTyrLeu 151015 GlnLeuLeuGluCysGluIleValLeuGlnAlaGlnLeuLysAsp 202530 AspAlaAsnSerGlnLysValLysGluPheLeuGlnGluIleVal 354045 AlaMetSerProMetIleSerLeuAspGluLysGluLeuProArg 505560 ThrProSerPheArgIleAlaLysLysGlyGlnGluSerGlyVal 657075 GluPheAlaGlyLeuProLeuGlyHisGluPheTyrPheValTyr 808590 LeuGlySerValThrGlyPheArgAlaSerAlaLysValGluThr 95100105 AspIleValLysArgIleGlnAlaValAspGluProMetHisPhe 110115120 GluThrTyrValSerLeuThrCysHisAsnCysProAspValVal 125130135 GlnAlaPheAsnIleMetSerValValAsnProAsnIleSerHis 140145150 ThrMetValGluGlyGlyMetPheLysAspGluIleGluAlaLys 155160165 GlyIleMetSerValProThrValTyrLysAspGlyThrGluPhe 170175180 ThrSerGlyArgAlaSerIleGluGlnLeuLeuAspLeuIleAla 185190195 GlyProLeuLysGluAspAlaPheAspAspLysGlyValPheAsp 200205210 ValLeuValIleGlyGlyGlyProAlaGlyAsnSerAlaAlaIle 215220225 TyrAlaAlaArgLysGlyValLysThrGlyLeuLeuAlaGluThr 230235240 MetGlyGlyGlnValMetGluThrValGlyIleGluAsnMetIle 245250255 GlyThrProTyrValGluGlyProGlnLeuMetAlaGlnValGlu 260265270 GluHisThrLysSerTyrSerValAspIleMetLysAlaProArg 275280285 AlaLysSerIleGlnLysThrAspLeuValGluValGluLeuAsp 290295300 AsnGlyAlaHisLeuLysAlaLysThrAlaValLeuAlaLeuGly 305310315 AlaLysTrpArgLysIleAsnValProGlyGluLysGluPhePhe 320325330 AsnLysGlyValThrTyrCysProHisCysAspGlyProLeuPhe 335340345 ThrAspLysLysValAlaValIleGlyGlyGlyAsnSerGlyLeu 350355360 GluAlaAlaIleAspLeuAlaGlyLeuAlaSerHisValTyrIle 365370375 LeuGluPheLeuProGluLeuLysAlaAspLysIleLeuGlnAsp 380385390 ArgAlaGluAlaLeuAspAsnIleThrIleLeuThrAsnValAla 395400405 ThrLysGluIleIleGlyAsnAspHisValGluGlyLeuArgTyr 410415420 SerAspArgThrThrAsnGluGluTyrLeuLeuAspLeuGluGly 425430435 ValPheValGlnIleGlyLeuValProSerThrAspTrpLeuLys 440445450 AspSerGlyLeuAlaLeuAsnGluLysGlyGluIleIleValAla 455460465 LysAspGlyAlaThrAsnIleProAlaIlePheAlaAlaGlyAsp 470475480 CysThrAspSerAlaTyrLysGlnIleIleIleSerMetGlySer 485490495 LysAlaThrAlaAlaLeuGlyAlaPheAspTyrLeuIleArgAsn 500505510
Claims (5)
1. A DNA molecular comprising nucleotides 279 through 1809 of sequence I.D. No. 1, wherein the molecule has the following base sequence:
______________________________________
AT GGCATTAGAC GCAGAAATCA
AAGAGCAGTT
AGGACAGTAT CTTCAATTAC
TTGAGTGTGA
GATTGTTTTA CAAGCTCAAT
TAAAAGACGA
TGCTAATTCT CAAAAAGTTA
AGGAATTTCT
CCAAGAAATC GTTGCAATGT
CTCCTATGAT
TTCTTTAGAC GAAAAGGAAC
TTCCGCGAAC
ACCTAGTTTT CGCATAGCTA
AAAAGGGGCA
AGAATCTGGT GTTGAATTTG
CTGGCTTACC
CCTTGGTCAC GAATTTTACT
TCGTTTATCT
TGGCTCTGTT ACAGGTTTCA
GGGCGTCGGC
TAAGGTAGAG ACTGATATTG
TCAAACGCAT
TCAAGCTGTT GATGAACCTA
TGCATTTTGA
AACCTATGTT AGTTTGACTT
GTCATAATTG
TCCAGATGTT GTTCAGGCTT
TCAATATCAT
GTCAGTTGTT AATCCCAACA
TTTCACATAC
AATGGTGGAA GGTGGCATGT
TTAAAGATGA
AATTGAAGCT AAGGGAATTA
TGTCTGTGCC
AACTGTCTAT AAAGATGGAA
CAGAATTTAC
CTGAGGGCGT GCTAGCATAG
AGCAATTACT
AGACTTGATA GCAGGTCCTG
TTAAAGAAGA
TGCTTTTGAT GATAAAGGTG
TTTTTGATGT
CCTTGTTATT GGTGGGGGTC
CTGCTGGTAA
TAGCGCGGCT ATCTATGCTG
CAAGAAAGGG
AGTTAAAACA GGACTTTTAG
CTGAAACCAT
GGGTGGTCAA GTTATGGAAA
CCGTGGGTAT
TGAAAATATG ATCGGTACCC
CATATGTTGA
AGGACCCCAA TTAATGGCTC
AGGTGGAAGA
GCATACCAAG TCTTATTCTG
TTGACATCAT
GAAGGCACCG CGTGCTAAGT
CTATTCAAAA
GACAGACTTG GTTGAAGTTG
AACTTGATAA
TGGAGCTCAT TTGAAAGCAA
AGACAGCTGT
TTTGGCCTTA GGTGCCAAGT
GGCGTAAAAT
CAATGTACCA GGAGAAAAAG
AATTCTTTAA
TAAAGGTGTT ACTTACTGTC
CGCACTGTGA
TGGTCCTCTT TTCACAGACA
AAAAAGTCGC
TGTCATTGGC GGTGGAAACT
CAGGTTTAGA
AGCAGCTATT GATTTGGCTG
GGTTAGCTAG
CCATGTCTAT ATTTTAGAAT
TTTTACCTGA
GTTAAAAGCT GATAAGATCT
TACAAGATCG
TGCGGAAGCT CTTGATAATA
TTACCATTGT
AACTAATGTT GCGACTAAAG
AAATTATTGG
CAATGACCAC GTAGAAGGTC
TTCGTTACAG
TGATCGTACG ACCAATGAAG
AGTACTTGCT
TGATTTAGAA GGTGTTTTTG
TTCAAATTGG
ATTGGTACCT AGTACTGACT
GGTTAAAGGA
TAGTGGACTA GGACTCAATG
AAAAAGGTGA
AATCATTGTT GCTAAAGATG
GCGCAACTAA
TATTCCTGCT ATTTTTGCAG
CTGGTGATTG
CACAGATAGT GCCTACAAAC
AAATTATCAT
TTCCATGGGT TCTGGAGCTA
CTGCGGCTTT
AGGTGCCTTT GATTATTTGA
TTAGAAATT/.
______________________________________
2. A DNA molecule comprising the amino acid sequence I.D. No. 2, wherein the molecule encodes the following amino acid sequence:
__________________________________________________________________________
Met
Ala
Leu
Asp
Ala
Glu
Ile
Lys
Glu
Gln
Leu
Gly
Gln
Tyr
Leu
15
Gln
Leu
Leu
Glu
Cys
Glu
Ile
Val
Leu
Gln
Ala
Gln
Leu
Lys
Asp
30
Asp
Ala
Asn
Ser
Gln
Lys
Val
Lys
Glu
Phe
Leu
Gln
Glu
Ile
Val
45
Ala
Met
Ser
Pro
Met
Ile
Ser
Leu
Asp
Glu
Lys
Glu
Leu
Pro
Arg
60
Thr
Pro
Ser
Phe
Arg
Ile
Ala
Lys
Lys
Gly
Gln
Glu
Ser
Gly
Val
75
Glu
Phe
Ala
Gly
Leu
Pro
Leu
Gly
His
Glu
Phe
Tyr
Phe
Val
Tyr
90
Leu
Gly
Ser
Val
Thr
Gly
Phe
Arg
Ala
Ser
Ala
Lys
Val
Glu
Thr
105
Asp
Ile
Val
Lys
Arg
Ile
Gln
Ala
Val
Asp
Glu
Pro
Met
His
Phe
120
Glu
Thr
Tyr
Val
Ser
Leu
Thr
Cys
His
Asn
Cys
Pro
Asp
Val
Val
135
Gln
Ala
Phe
Asn
Ile
Met
Ser
Val
Val
Asn
Pro
Asn
Ile
Ser
His
150
Thr
Met
Val
Glu
Gly
Gly
Met
Phe
Lys
ASp
Glu
Ile
Glu
Ala
Lys
165
Gly
Ile
Met
Ser
Val
Pro
Thr
Val
Tyr
Lys
Asp
Gly
Thr
Glu
Phe
180
Thr
Ser
Gly
Arg
Ala
Ser
Ile
Glu
Gln
Leu
Leu
Asp
Leu
Ile
Ala
195
Gly
Pro
Leu
Lys
Glu
Asp
Ala
Phe
Asp
Asp
Lys
Gly
Val
Phe
Asp
210
Val
Leu
Val
Ile
Gly
Gly
Gly
Pro
Ala
Gly
Asn
Ser
Ala
Ala
Ile
225
Tyr
Ala
Ala
Arg
Lys
Gly
Val
Lys
Thr
Gly
Leu
Leu
Ala
Glu
Thr
240
Met
Gly
Gly
Gln
Val
Met
Glu
Thr
Val
Gly
Ile
Glu
Asn
Met
Ile
255
Gly
Thr
Pro
Tyr
Val
Glu
Gly
Pro
Gln
Leu
Met
Ala
Gln
Val
Glu
270
Glu
His
Thr
Lys
Ser
Tyr
Ser
Val
Asp
Ile
Met
Lys
Ala
Pro
Arg
285
Ala
Lys
Ser
Ile
Gln
Lys
Thr
Asp
Leu
Val
Glu
Val
Glu
Leu
Asp
300
Asn
Gly
Ala
His
Leu
Lys
Ala
Lys
Thr
Ala
Val
Lue
Ala
Lue
Gly
315
Ala
Lys
Trp
Arg
Lys
Ile
Asn
Val
Pro
Gly
Glu
Lys
Glu
Phe
Phe
330
Asn
Lys
Gly
Val
Thr
Tyr
Cys
Pro
His
Cys
Asp
Gly
Pro
Leu
Phe
345
Thr
Asp
Lys
Lys
Val
Ala
Val
Ile
Gly
Gly
Gly
Asn
Ser
Gly
Leu
360
Glu
Ala
Ala
Ile
Asp
Leu
Ala
Gly
Leu
Ala
Ser
His
Val
Tyr
Ile
375
Leu
Glu
Phe
Leu
Pro
Glu
Leu
Lys
Ala
Asp
Lys
Ile
Leu
Gln
Asp
390
Arg
Ala
Glu
Ala
Lue
Asp
Asn
Ile
Thr
Ile
Leu
Thr
Asp
Val
Ala
425
Thr
Lys
Glu
Ile
Ile
Gly
Asn
Asp
His
Val
Glu
Gly
Leu
Arg
Tyr
420
Ser
Asp
Arg
Thr
Thr
Asn
Glu
Glu
Tyr
Leu
Leu
Asp
Leu
Glu
Gly
435
Val
Phe
Val
Gln
Ile
Gly
Leu
Val
Pro
Ser
Thr
Asp
Trp
Leu
Lys
450
Asp
Ser
Gly
Leu
Ala
Leu
Asn
Glu
Lys
Gly
Glu
Ile
Ile
Val
Ala
465
Lys
Asp
Gly
Ala
Thr
Asn
Ile
Pro
Ala
Ile
Phe
Ala
Ala
Gly
Asp
480
Cys
Thr
Asp
Ser
Ala
Tyr
Lys
Gln
Ile
Ile
Ile
Ser
Met
Gly
Ser
495
Lys
Ala
Thr
Ala
Ala
Leu
Gly
Ala
Phe
Asp
Tyr
Leu
Ile
Arg
Asn
510.
__________________________________________________________________________
3. A DNA molecule wherein the 5' terminal non-translated region comprises the following base sequence:
__________________________________________________________________________
GGATCCCTTC
TCATGTTCTC
TCACAAGGAT
TTGAGGTTTT
AGGTGAAGAT
GGTTTAGCAC
60
AACGTGGAAC
CTTTATTGTA
GATCCGGATG
GTATCATTCA
AATGATGGAA
GTCAATGCAG
120
ATGGTATTGG
TCGTGATGCT
ACTACCTTGA
TTGATAAAGT
TGTGCAGCT CAATCTATTC
180
GCCAACATCC
AGGAGAAGTT
TGCCCTGCCA
AATGGAAAGA
GGGAGCTGAA
ACTTTAAAAC
240
CAAGTTTGGT
ACTTGTCGGT
AAAATTTAAG
GAGAAACT.
__________________________________________________________________________
4. A plasmid including a DNA molecule encoding an NADH oxidase according to claim 1.
5. A plasmid including a DNA molecule encoding an NADH oxidase according to claim 2.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5-073989 | 1993-03-31 | ||
| JP7398993 | 1993-03-31 | ||
| JP25445993 | 1993-10-12 | ||
| JP5-254459 | 1993-10-12 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| US5514587A true US5514587A (en) | 1996-05-07 |
Family
ID=26415131
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| US08/220,677 Expired - Fee Related US5514587A (en) | 1993-01-12 | 1994-03-31 | DNA fragment encoding a hydrogen peroxide-generating NADH oxidase |
Country Status (2)
| Country | Link |
|---|---|
| US (1) | US5514587A (en) |
| EP (1) | EP0623677A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110045548A1 (en) * | 2008-01-17 | 2011-02-24 | Keio University | Novel hydrogen peroxide-forming nadh oxidase and dna encoding the same |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3953578B2 (en) * | 1997-05-09 | 2007-08-08 | ユニチカ株式会社 | Thermostable diaphorase gene |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3725851A1 (en) * | 1986-08-12 | 1988-02-18 | Takara Shuzo Co | NAD (P) H-OXIDASE, METHOD FOR ITS INSULATION AND APPLICATION |
| EP0385415A1 (en) * | 1989-02-28 | 1990-09-05 | Mitsubishi Petrochemical Co., Ltd. | Process for producing NADH oxidase |
| JPH04365478A (en) * | 1990-12-27 | 1992-12-17 | Nippon Paint Co Ltd | Nadh oxidase and its production |
| DE4221830A1 (en) * | 1991-07-25 | 1993-01-28 | Biotechnolog Forschung Gmbh | Escherichia coli expression vector for NADH-oxidase gene - derived from 26.8kD gene isolated from Thermus thermophilus, useful as highly stable bio-sensor |
| JPH05344890A (en) * | 1992-06-15 | 1993-12-27 | Mitsubishi Petrochem Co Ltd | Gene capable of coding nadh oxidase and its utilization |
-
1994
- 1994-03-31 EP EP94105134A patent/EP0623677A1/en not_active Withdrawn
- 1994-03-31 US US08/220,677 patent/US5514587A/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3725851A1 (en) * | 1986-08-12 | 1988-02-18 | Takara Shuzo Co | NAD (P) H-OXIDASE, METHOD FOR ITS INSULATION AND APPLICATION |
| EP0385415A1 (en) * | 1989-02-28 | 1990-09-05 | Mitsubishi Petrochemical Co., Ltd. | Process for producing NADH oxidase |
| JPH04365478A (en) * | 1990-12-27 | 1992-12-17 | Nippon Paint Co Ltd | Nadh oxidase and its production |
| DE4221830A1 (en) * | 1991-07-25 | 1993-01-28 | Biotechnolog Forschung Gmbh | Escherichia coli expression vector for NADH-oxidase gene - derived from 26.8kD gene isolated from Thermus thermophilus, useful as highly stable bio-sensor |
| JPH05344890A (en) * | 1992-06-15 | 1993-12-27 | Mitsubishi Petrochem Co Ltd | Gene capable of coding nadh oxidase and its utilization |
Non-Patent Citations (6)
| Title |
|---|
| "Identification of Two Distinct NADH Oxidases Corresponding to H202-forming Oxidase Induced in Streptococcus Mutans"; J. Gen. Microbiology; pp. 2343-2351; vol. 139, No. PT10; Oct. 1993. |
| "Molecular Cloning And Sequence Analysis Of The Gene Encoding The H202-Forming NADH Oxidase From Streptococcus Mutans"; Abstract; Feb. 11, 1994; Higuchi, et al.; Database EMBL. |
| Higuchi (1992) Oral Microbiol. Immunol. 7:309 314, Park, et al. (1992) Eur. J. Biochem. 205(3):875 879. * |
| Higuchi (1992) Oral Microbiol. Immunol. 7:309-314, Park, et al. (1992) Eur. J. Biochem. 205(3):875-879. |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110045548A1 (en) * | 2008-01-17 | 2011-02-24 | Keio University | Novel hydrogen peroxide-forming nadh oxidase and dna encoding the same |
| US8546113B2 (en) | 2008-01-17 | 2013-10-01 | Keio University | Hydrogen peroxide-forming NADH oxidase and DNA encoding the same |
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