TWI748207B - Sunblock liposome, and method for preparing the same - Google Patents

Abstract

The present disclosure provides a sunblock liposome prepared by the following steps. A first solution is provided, wherein the first solution is used as a water phase and includes resveratrol and at least one sunscreen. A second solution is provided, wherein the second solution is used as an oil phase and includes lecithin and cholesterol, wherein the lecithin and the surfactant are contained in a weight percentage of 10:1 to 30:1. A water-in-oil emulsification is performed, wherein the water-in-oil emulsification is for mixing the first solution and the second solution so as to obtain an emulsion. A homogenization step is performed, wherein the homogenization step is for homogenizing the emulsion so as to obtain the sunblock liposome. Therefore, the sunblock liposome can increase effectiveness of sunscreen and reduce the amount of chemical sunscreen, so that the sunblock liposome has a marketing potential in the related fields.

Description

Sunscreen liposome and preparation method thereof

The present invention relates to a liposome and a preparation method of liposomes, in particular to a sun-protecting liposome and a preparation method of the sun-protecting liposome.

Ultraviolet rays cause many serious damage to the skin, including skin redness, sunburn, pigmentation, aging, loss of elasticity and wrinkles, etc., and even damage the skin's immune system and increase the chance of skin cancer. Coupled with the destruction of the environment in recent years, the invasion of ultraviolet rays has become more and more serious, so the work of sun protection cannot be ignored.

Sunscreens are often added to cosmetics to filter out harmful ultraviolet radiation. Depending on the mechanism of action, sunscreens can be divided into chemical sunscreens and physical sunscreens. Chemical sunscreens are also so-called ultraviolet absorbers. Most of them are synthetic light-absorbing compounds that absorb ultraviolet energy to achieve sun protection. These compounds can absorb ultraviolet rays of specific wavelengths depending on their chemical structure. Physical sunscreens mainly resist sunlight through the reflection and scattering of sunlight by solid particles, and prevent sunlight from directly contacting the skin. Therefore, inorganic particles are generally used as physical sunscreens.

Although sunscreens help resist the damage of ultraviolet rays, they also have many side effects, including skin irritation, inflammation, photosensitivity, and phototoxic reactions. Among them, chemical sunscreens use energy transfer to achieve the effect of sun protection, but some sunscreens will photodegrade due to the energy provided by ultraviolet rays, produce harmful free radicals and phototoxic substances, and also reduce the sunscreen ability of the product. . In addition, 18.1% of Chinese people's contact allergies are caused by discomfort after using sunscreens. In recent years, many sunscreen ingredients have been proposed to have endocrine activity.

Therefore, how to develop a sunscreen material that can effectively achieve the sunscreen effect, reduce the side effects on the skin and reduce the load on the environment, is the goal of the joint efforts of those skilled in the art.

One aspect of the present invention is to provide a method for preparing sunscreen liposomes, which includes the following steps: providing a first solution, providing a second solution, performing a water-in-oil emulsification reaction, and performing a homogenization step. The first solution is used as an aqueous phase and contains a resveratrol and at least one sunscreen. The second solution is used as an oil phase and contains a lecithin and a cholesterol, wherein the lecithin and the cholesterol are mixed in a ratio of 10:1 to 30:1 by weight. The water-in-oil type emulsification reaction system mixes the first solution and the second solution to form an emulsion. The homogenization step is to homogenize the emulsion with a homogenizer to prepare a sunscreen liposome.

According to the aforementioned method for preparing sunscreen liposomes, at least one sunscreen can be selected from para-aminobenzoic acid sunscreens and benzophenone sunscreens. A group consisting of cinnamate sunscreens and salicylate sunscreens.

According to the aforementioned method for preparing sunscreen liposomes, at least one sunscreen agent can be octyl methoxycinnamate, benzophenone-3 or butylmethoxybenzylmethane.

According to the aforementioned preparation method of sunscreen liposomes, in the water-in-oil type emulsification reaction, the first solution and the second solution can be mixed by ultrasonic vibration and stirring.

According to the aforementioned method for preparing sunscreen liposomes, the aforementioned homogenizer can be a high-pressure homogenizer, and the high-pressure homogenization pressure of one of the high-pressure homogenizers can be 400 bar to 1500 bar.

Another aspect of the present invention is to provide a sunscreen liposome, which is prepared by the aforementioned preparation method.

According to the aforementioned sunscreen liposome, an average particle size of the aforementioned sunscreen liposome can be 200nm to 400nm.

According to the aforementioned sunscreen liposome, a zeta potential of the sunscreen liposome can be -60mV to -40mV.

According to the aforementioned sunscreen liposome, the coating rate of resveratrol can be 45% to 60%.

According to the aforementioned sunscreen liposome, the coating rate of at least one sunscreen agent can be 35% to 60%.

Thereby, the preparation process of the sunscreen liposome of the present invention is simple, and the prepared sunscreen liposome can increase the sunscreen performance of chemical sunscreen agents, reduce the amount of chemical sunscreen agents, and reduce chemical sunscreen agents The irritation caused by transdermal absorption has the potential to be applied to the cosmetics market.

100‧‧‧Preparation method of sunscreen liposome

110‧‧‧Provide a first solution

120‧‧‧Provide a second solution

130‧‧‧Perform a water-in-oil type emulsification reaction

140‧‧‧Perform a homogenization step

300‧‧‧Sunscreen Liposome

In order to make the above and other objectives, features, advantages and embodiments of the present invention more comprehensible, the description of the accompanying drawings is as follows: Figure 1 shows the steps of a method for preparing sunscreen liposomes according to an embodiment of the present invention Flow chart; Figure 2 shows a penetrating electron microscope image of a sunscreen liposome according to another embodiment of the present invention; Figures 3 and 4 show the in vitro sunscreen liposome of Example 1 of the present invention The penetration curve of transdermal delivery; Figures 5 and 6 show the penetration curve of the sunscreen liposome of Example 2 of the present invention for transdermal delivery in vitro; Figure 7 shows the implementation of the present invention The results of the analysis of the sun protection ability of the sunscreen preparation containing sunscreen liposomes of Example 3; and Fig. 8 is a graph showing the results of the analysis of the sun protection ability of the sunscreen preparation containing sunscreen liposomes of Example 4 of the present invention.

The following is an explanation of the specific terms used in this specification: The " para- aminobenzoic acid sunscreen" mentioned in the specification refers to a sunscreen containing para aminobenzoic acid or its derivatives, which is a chemical Sunscreen. Para-aminobenzoic acid sunscreens can absorb ultraviolet rays with a wavelength of 200~313nm. Previous studies have shown that para-aminobenzoic acid sunscreens can cause skin inflammation and photosensitivity after being irradiated with ultraviolet rays. In addition, after photolysis, The product is considered a carcinogen.

The "benzophenone sunscreen agent" mentioned in the manual refers to a sunscreen agent containing benzophenones, which is a chemical sunscreen agent, benzophenone-3 (BP-3) It is one of the benzophenone sunscreens. Benzophenone sunscreens are an important class of ultraviolet absorbers. There are two types based on structural classification. One has only one hydroxyl group and can absorb ultraviolet rays with a wavelength of 290-380nm. The other contains two hydroxyl groups, the absorption band shifts to the long-wave direction, and can absorb ultraviolet light with a wavelength of 300 to 400 nm. Therefore, benzophenone ultraviolet absorption can absorb both UVA and UVB sections, and it is a kind of broad-spectrum ultraviolet absorber. But it is prone to oxidation reaction. It has good affinity with the skin and mucous membranes, and is prone to light allergic reactions.

The "cinnamate sunscreens" mentioned in the instructions refer to sunscreens containing cinnamates, which are chemical sunscreens. Octyl methoxycinnamate (OMC) is one of the cinnamates. Salt sunscreens. Cinnamic acid salt sunscreens are ultraviolet UVB absorbers. In order to improve their sunscreen effect, they are often used in conjunction with other ultraviolet absorbers. They are currently the most widely used and the largest amount of sunscreens. It is often used in conjunction with UVA ultraviolet absorbers to achieve full sun protection. In the pure compound state, the light stability of OMC is relatively high, but in the emulsification system of sunscreen cosmetics, especially in the presence of water and surfactants, the light stability of OMC under ultraviolet light is not very high. 10 MED After the minimum erythema dose is irradiated with sunlight, more than half of the active ingredients are degraded. The photodegradation products of OMC will have a certain irritation effect on the skin. In recent years, some animal studies have pointed out that OMC may be an estrogen-like component.

The "salicylate sunscreens" mentioned in the instructions refer to sunscreens containing salicylates. It is a chemical sunscreen and is an older type of ultraviolet absorber, which is characterized by its low price. , Low toxicity, good compatibility with other ingredients. The disadvantage is that the ultraviolet absorption rate is low, and its absorption band is relatively narrow (under 340nm), and it is not very stable to ultraviolet rays. After long-term exposure, the rearrangement reaction occurs, and the visible light is obviously absorbed to make it colored.

The "butyl methoxydibenzylmethane (B-MDM)" described in the manual is also known as Avobenzone or Parsol 1789. It is a major ultraviolet UVA sunscreen. It is a chemical sunscreen and can absorb UVA. The 320~400 band can block some UVA-I, but the effect on UVA-II is weak, so it is often mixed with BP-3, which can easily cause skin discomfort such as allergies. In addition, the main disadvantage of butylmethoxydibenzamethane is its low light stability, and it will gradually be decomposed after being irradiated by ultraviolet rays and lose its sunscreen effect.

<Preparation method of sunscreen liposome of the present invention>

Please refer to Fig. 1, which is a flowchart of the steps of a method 100 for preparing sunscreen liposomes according to an embodiment of the present invention. The method 100 for preparing sunscreen liposomes includes step 110, step 120, step 130, and step 140.

Step 110 is to provide a first solution, wherein the first solution is used as an aqueous phase and contains a resveratrol and at least one sunscreen. White Chenopodium The retinol and at least one sunscreen are dissolved in a polar solution to form the aforementioned first solution. In detail, the aforementioned resveratrol (resveratrol) is a non-flavonoid phenolic substance, which belongs to the phytoantitoxin of stilbene compounds. The natural sources of resveratrol are grape, blueberry, raspberry and mulberry peels and Cassia plant. The at least one sunscreen can be selected from a group consisting of para-aminobenzoic acid sunscreens, benzophenone sunscreens, cinnamate sunscreens, and salicylate sunscreens. Preferably, at least one The sunscreen may be octyl methoxycinnamate, benzophenone-3 or butylmethoxybenzoylmethane.

Step 120 is to provide a second solution, wherein the second solution is used as an oil phase and contains a lecithin and a cholesterol, wherein the lecithin and the cholesterol are mixed in a ratio of 10:1 to 30:1 by volume. In detail, the aforementioned lecithin will form the phospholipid bilayer membrane structure of the sunscreen liposome, so that resveratrol and at least one sunscreen agent are coated therein, and the cholesterol molecules will be evenly distributed in the phospholipid bilayer membrane structure In the inner and outer membranes of the sunscreen liposomes, the phase transition temperature of the sunscreen liposomes can be changed to control the permeability and fluidity of the phospholipid bilayer membrane of the sunscreen liposomes. Preferably, the aforementioned lecithin may be Emulmetik 900.

Step 130 is to perform a water-in-oil type emulsification reaction, which involves mixing the first solution and the second solution to form an emulsion. Preferably, the first solution and the second solution are mixed by ultrasonic vibration and stirring, so that the first solution and the second solution can be uniformly dispersed and mixed.

Step 140 is to perform a homogenization step, which is to homogenize the aforementioned emulsion with a homogenizer to prepare a sunscreen liposome. Preferably, the aforementioned homogenizer can be a high-pressure homogenizer, and one of the high-pressure homogenizers can be a high-pressure homogenizer. It is 400 bar to 1500 bar to further adjust the average particle size, coating rate and zeta potential of the sunscreen liposome.

Thereby, the preparation method 100 of sunscreen liposomes of the present invention can effectively treat resveratrol and at least one sunscreen by mixing the lecithin and cholesterol contained in the second solution in a ratio of 10:1 to 30:1 by weight. The sunscreen liposome is coated with the sunscreen liposome, so that the sunscreen liposome prepared by the method 100 of the present invention has the characteristics of high variability, easy absorption, and appropriate average particle size, interface potential, and coating rate. Increase the sunscreen efficacy of chemical sunscreens to reduce the amount of chemical sunscreens and reduce the irritation caused by the transdermal absorption of chemical sunscreens. Preferably, the sunscreen liposome prepared by the aforementioned method 100 for preparing sunscreen liposome has an average particle size of 200nm to 400nm, and its interface potential can be -60mV to -40mV, wherein the coating of resveratrol The rate may be 45% to 60%, and the coating rate of at least one sunscreen agent may be 35% to 60%.

The following specific test examples are used to further illustrate the present invention, so as to facilitate those who are generally knowledgeable in the technical field of the present invention to fully utilize and practice the present invention without excessive interpretation. These test examples should not be regarded as It limits the scope of the present invention, but is used to illustrate how to implement the materials and methods of the present invention.

<Sunscreen liposome of the present invention>

In order to confirm that the sunscreen liposome preparation method 100 can indeed prepare the sunscreen liposome 300, in this test example, the sunscreen liposome 300 will be prepared through step 110 to step 140, and its structure will be confirmed with a penetrating electron microscope.

Please refer to FIG. 2, which is a transmission electron microscope image of the sunscreen liposome 300 according to another embodiment of the present invention. The results show that the sunscreen liposome 300 of the present invention is a uniform microsphere structure, which shows that the sunscreen liposome 300 can indeed be prepared by the method 100 for preparing the sunscreen liposome.

<Evaluation of the effect of high-pressure homogenization pressure on the physical properties of liposomes>

In this test example, first prepare liposomes containing resveratrol without adding cholesterol and at least one sunscreen (test example 1 to test example 5) to test the best high pressure of the high pressure homogenizer used in the homogenization step Homogenize the pressure, and analyze the average particle size and interface potential of Test Example 1 to Test Example 5 with a Laser Particle Size Analyzer (Malvern), and use a high-performance liquid chromatography (HPLC) system to evaluate Chenopodium album The coating rate of Retrol.

In the test of the average particle size of the liposomes containing resveratrol, first the liposomes containing resveratrol were diluted with deionized water and ultrasonically shaken for 5 minutes, and then a 1ml sample was taken as laser particles The diameter analyzer measures three times, and averages the three results to obtain the average particle diameter and the interface potential of the liposome containing resveratrol.

In the test of the coating rate of resveratrol, first the liposomes containing resveratrol were centrifuged at 10,000 rpm for 15 minutes, and then the supernatant was diluted with methanol and filtered with a membrane (PhreeTM Phospholipid Removal, Phenomenex) After filtration, the filtrate is analyzed by high performance liquid chromatograph, and the detection conditions of high performance liquid chromatograph are as follows: the injection volume is 50μL, and the detection wavelength is based on the test substance (in this test example, it is resveratrol). ) For adjustment, use 88% methanol + 11% water + 1% acetic acid as a mobile isocratic extraction, and the mobile phase flow rate is 1.0 mL/min. Adopt concentration 1 Resveratrol standard solution of ppm~100ppm establishes a calibration line, and each concentration is detected 3 times and the average value is taken. The signal acquisition and processing adopts peak-ABC tomography data processing system software (Great Tide Instruments, Taiwan), and formula I is used to calculate the coverage rate.

In this test example, the preparation conditions of Test Example 1 to Test Example 5 are prepared according to the method for preparing sunscreen liposomes of the present invention, but the first solution does not contain at least one sunscreen agent, and the second solution does not contain cholesterol , The detailed test conditions used in Test Example 1 to Test Example 5 are listed in Table 1.

The average particle size of the liposomes containing resveratrol of Test Example 1 to Test Example 5, the average particle size after two weeks, the coating rate of resveratrol, the coating rate and interface of resveratrol after two weeks The potential results are listed in Table 2.

It can be seen from the results in Table 2 that the higher the high-pressure homogenization pressure, the smaller the average particle size of the liposomes containing resveratrol, but the result of the difference in the coating rate of the liposomes containing resveratrol to resveratrol It is very small, showing that the high-pressure homogenizing pressure of the high-pressure homogenizer can effectively coat resveratrol under the condition of 400 bar to 1500 bar. Therefore, the method for preparing sunscreen liposomes of the present invention can use a high-pressure homogenizing pressure of 400 bar. The high-pressure homogenizer of bar to 1500 bar performs homogenization to obtain sunscreen liposomes with excellent average particle size, resveratrol coating rate and interface potential. However, the results in Table 2 show that when the high-pressure homogenization pressure is 600 bar, the best resveratrol coating rate can be obtained with resveratrol-containing liposomes (the coating rate is 43.93±0.37%). The high-pressure homogenization pressure in the subsequent test conditions was used to prepare sunscreen liposomes at 600 bar.

<Evaluation of the effect of cholesterol addition ratio on the physical properties of liposomes>

In this test example, first prepare resveratrol liposomes without adding at least one sunscreen agent (test example 6 to test example 8) to test the optimal mixing ratio of lecithin and cholesterol in the second solution, and use the lecithin The particle size analyzer analyzes the average particle size and interface potential of Test Example 6 to Test Example 8, and uses a high performance liquid chromatography (HPLC) system to evaluate the coating rate of resveratrol. For the analysis method of the coating rate, average particle size and interface potential of resveratrol, please refer to the previous paragraph, so I will not repeat it here.

In this test example, the preparation conditions of Test Example 6 to Test Example 8 are prepared according to the method for preparing sunscreen liposomes of the present invention, and the high-pressure homogenization pressure in the homogenization step is 600 bar, but the first solution does not contain At least one sunscreen, and the detailed test conditions used in Test Example 6 to Test Example 8 are listed in Table 3.

Results of the average particle size of the resveratrol liposomes of Test Example 6 to Test Example 8, the average particle size after two weeks, the coating rate of resveratrol, the coating rate of resveratrol and the interface potential after two weeks Listed in Table 4.

It can be seen from the results in Table 4 that the results of the resveratrol liposomes of Test Example 6 to Test Example 8 in the average particle size, the coating rate of resveratrol and the interface potential are very small, indicating that the lecithin in the second solution Resveratrol can be effectively coated under the conditions of mixing with a ratio of 10:1 to 30:1 by weight of cholesterol, which is the weight percentage of lecithin and cholesterol in the method for preparing sunscreen liposomes of the present invention It can be mixed in a ratio of 10:1 to 30:1, and a sunscreen liposome with excellent average particle size, resveratrol coating rate and interface potential can be obtained. However, the results in Table 4 show that when the weight percentage of lecithin and cholesterol is 15:1, resveratrol liposomes with the best resveratrol coating rate can be obtained (the coating rate is 42.25±0.47%) And after two weeks of storage at room temperature, the coating rate of resveratrol remains roughly constant. Therefore, in the subsequent test conditions, the weight percentage of lecithin and cholesterol in the second solution is 15:1 The preparation of sunscreen liposomes is carried out.

<Example>

The example is to prepare the sunscreen liposome of the present invention under the aforementioned optimal preparation conditions, and in Example 1, at least one sunscreen is OMC, and in Example 2, at least one sunscreen is OMC and B-MDM. In addition, Comparative Example 1 with no cholesterol added was prepared as a control group. The prepared sunscreen liposomes of Example 1, Example 2 and Comparative Example 1 were further subjected to average particle size analysis, interface potential analysis, resveratrol coating rate analysis, and sunscreen coating rate analysis. For the analysis methods of the coating rate, average particle size and interface potential of resveratrol, please refer to the previous paragraph, which will not be repeated here. The analysis method of the coating rate of sunscreen is the same as that of resveratrol, but the analyte is replaced with a sunscreen from resveratrol.

The detailed test procedure is as follows: weigh different proportions of resveratrol, OMC and B-MDM and dissolve them in 5% by weight ethanol aqueous solution, which is the first solution of the present invention. Then weigh different proportions of lecithin and cholesterol and dissolve them in 5% by weight ethanol aqueous solution, which is the second solution of the present invention. Next, mix the second solution and the first solution at a volume percentage of 1:1, stir evenly with a DC mixer (EURO-ST D S1 So, IKA, Germany) at 500 rpm, and ultrasonic vibration (DC300H, DELTA, Taiwan) After hours, the first solution and the second solution were uniformly dispersed to form an emulsion, and the aforementioned emulsion was oscillated with intermittent ultrasound for 2 minutes, and then homogenized at high pressure with a high-pressure homogenizer (APV-2000, SPXFLOW, Germany) The homogenization was performed 5 times under the condition of a pressure of 600 bar to prepare the sunscreen liposome of the present invention. The detailed preparation conditions of Example 1, Example 2 and Comparative Example 1 of the present invention are listed in Table 5.

Please refer to Table 6, which is the average particle size of the sunscreen liposomes of Example 1, Example 2 and Comparative Example 1, the average particle size after two weeks, the coating rate of resveratrol, and the resveratrol after two weeks. Test results of the coverage rate, interface potential, OMC coverage rate, OMC coverage rate after two weeks, B-MDM coverage rate and B-MDM coverage rate after two weeks.

From the results in Table 6, it can be seen that the coating rate of resveratrol in the sunscreen liposomes of Example 1 and Example 2 reached more than 50% (50.07±0.67% and 55.09±0.98%, respectively), and at room temperature After two weeks of storage, the coating rate of resveratrol remained above 40%. In addition, the OMC coating rate of the sunscreen liposomes of Example 1 and Example 2 is about 40% (39.77±0.51% and 40.10±0.08%, respectively), and after two weeks of storage at room temperature, the OMC The coverage rate is still maintained at more than 30%. In Example 2, where OMC and B-MDM are simultaneously coated, the coverage rate of B-MDM is also as high as 50.45±0.21%. Shows that the preparation method of sunscreen liposomes of the present invention is not It can only effectively coat resveratrol and at least one sunscreen, and the sunscreen liposomes prepared therefrom also have good stability, which is conducive to subsequent applications.

<Measurement of the transdermal delivery ability of sunscreen liposomes>

This test example further used in vitro transdermal transmission analysis to test whether the sunscreen liposome of the present invention has the penetrating properties that affect the human body, so as to evaluate the skin sensitization doubt of the sunscreen liposome of the present invention.

The test procedure for in vitro transdermal transmission analysis is as follows. First clean the pig ear skin with deionized water, take the pig ear epithelium with a scalpel (to remove the fat layer), and then cut it into a ^{penetrating membrane with an area of 1.5×1.5cm 2} and a thickness of 650 μm. After soaking the bag with PBS Frozen for later use. Before the test, the penetrating membrane was immersed in PBS physiological solution to thaw to room temperature to restore the natural state of swelling between the keratinocytes, and the penetrating membrane was fixed in the Franz-type diffusion cell (LOGAN FDC-6, USA), the diffusion area is 0.636cm ² , in order to test the integrity of the skin, the upper and lower layers of the diffusion tank are filled with 50% ethanol + 50% PBS, and the bubbles in the lower tank are removed, and then placed on the upper donor cell. 0.5g sample, the tested samples include Example 1, Example 2 and Comparative Example 1, and OMC, OMC-B-MDM and OMC-1789 were used as the control group. Place 50% ethanol + 50% PBS in the lower drug-receiving end (receptor cell, volume 5.3mL), use a constant temperature water tank heater, control the water temperature at 35℃±1, and stir it with a magnet to balance the drug-receiving end Drug concentration distribution. In each experiment, 5 diffusion tanks were used for measurement at the same time, and the average value was taken. A sample of 50 μL from the drug-receiving end was analyzed by HPLC, and then the same volume of physiological solution was backfilled to ensure that the volume in the drug-receiving end remained unchanged. In each experiment, 5 diffusion tanks were used for measurement at the same time, and the average value was taken. A sample of 50 μL from the drug-receiving end was analyzed by HPLC, and then the same volume of physiological solution was backfilled to ensure that the volume in the drug-receiving end remained unchanged.

Please refer to Figure 3, Figure 4, Figure 5 and Figure 6, where Figure 3 and Figure 4 are graphs showing the in vitro transdermal penetration curve of the sunscreen liposome of Example 1 of the present invention. The control group in Figures 3 and 4 is OMC. Figures 5 and 6 are graphs showing the penetration curve of the sunscreen liposomes in vitro for transdermal delivery of Example 2 of the present invention. The control group in Figure 5 is OMC-B-MDM, and the control in Figure 6 The group is OMC-1789.

The results in Figures 3 and 4 show that OMC has percutaneous penetration after 0.5 hours of the test, but neither Example 1 nor Comparative Example 1 has a rupture release in terms of percutaneous penetration. The results in Figure 5 show that OMC-B-MDM has percutaneous penetration after 0.5 hours of the test, but the cumulative permeability of Example 2 during the 6 hours of the test is less than 0.1 μg/cm ² . The results in Figure 6 show that OMC-1789 has percutaneous penetration after 1 hour of the test, but there is no rupture and release in the percutaneous penetration of Example 2. The above results show that the sunscreen liposomes of the present invention can indeed achieve long-term protection, and will not cause sensitization due to transdermal absorption.

<Analysis of sunscreen ability of sunscreen liposomes>

In this test example, the sunscreen liposome of the present invention is further prepared as a sunscreen preparation, and the sunscreen ability of the sunscreen liposome of the present invention is analyzed by using in vitro ultraviolet protection ability analysis. The in vitro UV protection ability analysis system uses Optomeric SPF-290S sun protection factor tester to measure the in vitro SPF value (sun protection factor) and explore the defense against UV rays. During the in vitro test, 3M (Transpore ^TM Tape) was used as artificial leather to quantitatively apply 2mg/cm ² sunscreen to receive the solar simulation lamp of the SPF meter, and the SPF evaluation parameters were calculated from the test results.

Please refer to Table 7 for the detailed weight percentage ratio of the sunscreen preparation containing the sunscreen liposome of the present invention.

Please refer to Figure 7, Figure 8, Table 8 and Table 9. Figure 7 shows the results of the analysis of the sun protection ability of the sunscreen formulation containing sunscreen liposomes according to Example 3 of the present invention, and Figure 8 shows the present invention. The results of the analysis of the sunscreen ability of the sunscreen formulation containing sunscreen liposomes in Example 4. Table 8 shows the analysis results of the sunscreen ability of the sunscreen formulation containing sunscreen liposomes of Example 3, and Table 9 shows the results of the sunscreen liposome containing sunscreen of Example 4 The analysis result of sunscreen ability of sunscreen preparation.

The results in Figure 7 and Table 8 show that the SPF value of Example 3 is 8.45±0.06, which is better than the SPF values of Comparative Examples 2 and 3 (7.60±0.09 and 8.17±0.04, respectively). After being damaged by the ultraviolet ray simulator, the sun protection factor of Example 3 decreased by 6.11%, and the sun protection factors of Comparative Example 2 and Comparative Example 3 decreased by 8.91% and 8.12%, respectively. Example 3 can effectively protect OMC from degradation, showing that the present invention The sun protection effect and durability of Example 3 are better than those of OMC without liposomes.

The results in Figure 8 and Table 9 show that the SPF value of Example 4 is 15.59±0.32, which is better than the SPF value of Comparative Example 4 (14.55±0.18). After being damaged by the ultraviolet ray simulator, the sun protection factor of Example 4 was reduced by 38%, and the sun protection factor of Comparative Example 4 was reduced by 44.92%. Example 4 can effectively protect OMC-B-MDM from degradation, showing an example of the present invention The sun protection effect and durability of 4 are better than that of OMC-B-MDM without liposomes.

In summary, the present invention provides a sunscreen liposome and a preparation method of the sunscreen liposome. The preparation method of the sunscreen liposome of the present invention has a simple process, and the prepared sunscreen liposome is a novel sunscreen material. Furthermore, the preparation method of sunscreen liposomes of the present invention can effectively treat resveratrol and at least one sunscreen by mixing the lecithin and cholesterol contained in the second solution in a ratio of 10:1 to 30:1 by weight. The sunscreen liposome is coated with the sunscreen liposome, so that the sunscreen liposome prepared by the method 100 of the present invention has the characteristics of high variability, easy absorption, and appropriate average particle size, interface potential, and coating rate. Increasing the sunscreen efficacy of chemical sunscreens, reducing the amount of chemical sunscreens, and reducing the irritation caused by the transdermal absorption of chemical sunscreens, has the potential to be applied to the cosmetics market.

Although the present invention has been disclosed in the above embodiments, it is not intended to limit the present invention. Anyone who is familiar with the art can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, the protection of the present invention The scope shall be subject to the scope of the attached patent application.

100‧‧‧Preparation method of sunscreen liposome

110‧‧‧Provide a first solution

120‧‧‧Provide a second solution

130‧‧‧Perform a water-in-oil type emulsification reaction

140‧‧‧Perform a homogenization step

Claims (7)

A method for preparing sunscreen liposomes includes the following steps: providing a first solution, wherein the first solution is used as an aqueous phase and contains a resveratrol and octyl methoxycinnamate, wherein the resveratrol and The octyl methoxycinnamate is mixed in a ratio of 1:100 by weight; a second solution is provided, which is used as an oil phase and contains a lecithin and a cholesterol, wherein the lecithin and the cholesterol are Mix in a ratio of 10:1 to 30:1 by weight; perform a water-in-oil emulsification reaction, which is to mix the first solution and the second solution to form an emulsion; and perform a homogenization step, which is A homogenizer homogenizes the emulsion to prepare a sunscreen liposome, wherein the homogenizer is a high-pressure homogenizer, and a high-pressure homogenization pressure of the high-pressure homogenizer is 400 bar to 1500 bar. According to the method for preparing sunscreen liposomes as described in item 1 of the scope of patent application, in the water-in-oil emulsification reaction, the first solution and the second solution are mixed by ultrasonic vibration and stirring. A sunscreen liposome, which is prepared by the preparation method according to any one of items 1 to 2 of the scope of patent application. The sunscreen liposome described in item 3 of the scope of patent application, wherein an average particle size of the sunscreen liposome is 200nm to 400nm. The sunscreen liposome described in item 3 of the scope of patent application, wherein an interface potential of the sunscreen liposome is -60mV to -40mV. The sunscreen liposome described in item 3 of the scope of patent application, wherein the coating rate of the resveratrol is 45% to 60%. The sunscreen liposome described in item 3 of the scope of patent application, wherein the coating rate of the octyl methoxycinnamate is 35% to 60%.

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