TWI685648B - The stratum corneum adopting tool and the stratum corneum adopting detection kit - Google Patents

Abstract

The invention obtains a kit for detecting the cuticle that can detect and measure the specific components of the cuticle of the skin with high accuracy.

The set includes: a stratum corneum taking tool 10, which includes a rod-shaped portion 11 having a grip portion 12 gripped by a user at one end, a cylindrical portion 15 connected to the other end of the rod-shaped portion 11, and formed The adhesive layer 16 on the peripheral surface of the columnar portion 15; and the solvent container 20, which is a bottomed cylindrical container with an opening in the upper part, and contains the solvent to be extracted into the horny layer of the adhesive layer 16 The columnar portion 15 with the tool 10 is taken from the above-mentioned opening to accommodate the stratum corneum in such a way that the adhesive layer 16 can be dipped in the solvent. In addition, the following parts are provided: the lids 13 and 14 which are immersed in the solvent in the adhesive layer 16 to close the opening of the solvent container 20; and the solvent extraction port 13a formed on the lid in such a manner that the closed state can be maintained 13 and 14, and the cap is opened to remove the solvent S from the solvent container 20 to the outside of the container.

Description

The stratum corneum adopting tool and the stratum corneum adopting detection kit

The invention relates to a horny layer taking tool for taking the horny layer of skin.

Furthermore, the present invention relates to a kit for detecting a stratum corneum layer for detecting specific components from the stratum corneum layer of skin.

Previously, as shown in Patent Documents 1, 2, and 3, for example, it was revealed that a specific component is detected from the stratum corneum of the skin, and the skin properties are judged at a biochemical level based on the measured amount of the component, that is, the rough skin state and the incomplete keratinization state Wait. Examples of skin characteristics determined in this manner include rough skin, such as skin aging caused by ultraviolet radiation, dry skin caused by a decrease in skin moisturizing power accompanied by a decrease in skin barrier function, and psoriasis or atopic dermatitis. Incomplete keratosis, rough skin caused by hay fever, etc.

More specifically, Patent Documents 1 and 2 disclose: Squamos Cell Carcinoma Antigen (Squamos Cell Carcinoma Antigen: hereinafter referred to as "SCCA") to skin stratum corneum cells, and more specifically to SCCA-1 And/or SCCA-2, especially SCCA-1, is quantitatively or qualitatively measured to determine the sensitivity, reactivity, and sensitivity of the skin.

In addition, Patent Document 3 discloses that by measuring the abundance of nerve growth factor and/or nerve nourishing factor 4 in a stratum corneum sample, skin diseases or sensitive skin can be checked.

In order to determine the skin properties as described above, it is necessary to first take a skin cuticle sample. Patent Documents 1 to 3 disclose that a skin cuticle sample is taken by a so-called tape peeling method. The so-called tape peeling method is to peel off the skin after attaching the tape sheet The horny layer of the skin is attached to the peeled tape and the horny layer sample method is adopted.

[Prior Technical Literature] [Patent Literature]

[Patent Document 1] Japanese Patent No. 5059600

[Patent Document 2] Japanese Patent Laid-Open No. 2007-279024

[Patent Document 3] Japanese Patent Laid-Open No. 2004-028698

Although the tape peeling method can be easily implemented, on the other hand, there is room for improvement in the accuracy of detecting specific components of the stratum corneum of the skin. That is, the tape sheet that peels off and maintains the cuticle of the skin is to be immersed in a solvent that extracts specific components of the cuticle of the skin. To this end, a certain amount of solvent must be stored in some containers in advance. Moreover, if the specific components of the stratum corneum of the skin are extracted into a relatively large amount of solvents in this way, the concentration of the specific components in the solvent becomes relatively low, so the detection accuracy of the specific components is easily impaired.

The present invention has been completed in view of the above circumstances, and an object of the present invention is to provide a horny layer collection kit capable of detecting and measuring specific components of the skin horny layer with high accuracy.

In addition, an object of the present invention is to provide a horny layer sampling tool for forming such a horny layer sampling detection kit.

The set for detecting the stratum corneum of the present invention is characterized by comprising: a stratum corneum sampling device, which includes a rod-shaped portion having a grip portion gripped by a user at one end portion and connected to the other end of the rod-shaped portion The columnar part and the adhesive layer formed on the peripheral surface of the columnar part; the solvent container, which is a bottomed cylindrical container with an opening at the upper part, and contains the ingredients to be taken from the stratum corneum of the adhesive layer Extracted solvent in the solvent The columnar portion of the stratum corneum collection tool can be accommodated from the opening by dipping the adhesive layer; and a lid body can be used to close the opening of the solvent container after dipping the adhesive layer in the solvent.

Here, in the kit for detecting the stratum corneum of the present invention, it is preferable to provide a solvent outlet, which is formed in the solvent container or the lid in such a manner as to maintain the closed state, and for the purpose of self-solvent container Remove the above solvent out of the container and open the cap.

In addition, in the set for detecting the stratum corneum of the present invention, it is more preferable that the cross-sectional area in the plane orthogonal to the cylinder axis direction of the internal space of the solvent container is set as compared with the portion where the above-mentioned opening is formed The cross-sectional area of the cylindrical part is smaller.

In addition, in the set for detection of the stratum corneum of the present invention, it is desirable to set the amount of the solvent so that the liquid level of the solvent in the solvent container is near the upper end of the adhesive layer immersed in the solvent.

In addition, in the set for detecting the stratum corneum of the present invention, it is preferable that the grip portion also serves as the lid.

Furthermore, in the horny layer acquisition detection kit of the present invention, it is more preferable to provide a guide mechanism that takes the horny layer inserted into the solvent container and adopts the adhesive layer so as not to contact the inner wall of the solvent container Positioning.

On the other hand, the stratum corneum adopting device of the present invention is characterized by comprising: a rod-shaped portion having a grip portion gripped by a user at one end; a cylindrical portion connected to the other end of the rod-shaped portion; And an adhesive layer, which is formed on the peripheral surface of the columnar portion.

The horny layer of the present invention is configured to immerse the adhesive layer formed on the peripheral surface of the columnar part in the solvent contained in the solvent container which is a cylindrical container, so the amount of solvent can be suppressed It is the minimum required within the range that the solvent can contact the adhesive layer. That is, the amount of the solvent can be set from the outside to the adhesion formed on the peripheral surface of the columnar portion The amount by which the layer is thinly enclosed. If the amount of solvent in the solvent container is suppressed to be small in this way, the specific component of the stratum corneum that is the object of detection and measurement is extracted into the solvent at a relatively high concentration, thereby performing the specific component with high precision Detection and determination.

1‧‧‧ The cuticle is used for detection kit

2‧‧‧The cuticles are tested

3‧‧‧The cuticle is used for detection

4‧‧‧The cuticles are tested

5‧‧‧The cuticle is used for detection

6‧‧‧The cuticles are tested

10‧‧‧ cuticle

11‧‧‧Bar-shaped part

12‧‧‧ grasping department

13‧‧‧bolt

13a‧‧‧Solvent export

13b‧‧‧Solvent outflow

14‧‧‧ cover

14a‧‧‧Hinge Department

15‧‧‧Columnar part

16‧‧‧adhesive layer

17‧‧‧ cover

20‧‧‧Solvent container

20a‧‧‧ opening

20b‧‧‧Male thread

20c‧‧‧taper

21‧‧‧Temporary hitch

30‧‧‧ grasping department

31‧‧‧Section

31a‧‧‧lock release section

31b‧‧‧Solvent export

32‧‧‧ cover

40‧‧‧bolt

40a‧‧‧Solvent export

40b‧‧‧Solvent outflow

42‧‧‧ Cover

50‧‧‧Solvent container

51‧‧‧Flange

51a‧‧‧Hinge Department

51b‧‧‧ opening

52‧‧‧cover

52a‧‧‧Hinge Department

52b‧‧‧Solvent outflow

52c

52d‧‧‧Cylindrical part

52e‧‧‧Solvent export

A‧‧‧Single point chain line

B‧‧‧Single point chain line

C‧‧‧Shake axis

H‧‧‧ Subject

M‧‧‧hand

S‧‧‧Solvent

Fig. 1 is a perspective view showing a kit for detecting cuticle acquisition according to a first embodiment of the present invention.

FIG. 2 is a schematic diagram illustrating a method of using the detection kit for the cuticle shown in FIG. 1.

FIG. 3 is a schematic diagram illustrating a method of using the cuticle layer detection kit shown in FIG. 1.

FIG. 4 is a perspective view showing a state when the cuticle shown in FIG. 1 is used in the detection kit.

FIG. 5 is a perspective view showing another state when the cuticle shown in FIG. 1 is used in the detection kit.

Fig. 6 is a perspective view showing a kit for detecting a cuticle layer according to a second embodiment of the present invention.

Fig. 7 is a perspective view showing a kit for detecting a stratum corneum according to a third embodiment of the present invention.

FIG. 8 is a schematic diagram illustrating a method of using the cuticle layer detection kit shown in FIG. 7.

FIG. 9 is a perspective view showing a state when the cuticle shown in FIG. 7 is used in the detection kit.

FIG. 10 is a perspective view showing another state when the cuticle shown in FIG. 7 is used in the detection kit.

Fig. 11 is a perspective view showing a kit for detecting cuticle acquisition according to a fourth embodiment of the present invention.

FIG. 12 is a schematic diagram illustrating a method of using the cuticle layer detection kit shown in FIG. 11.

FIG. 13 is a perspective view showing a state when the cuticle shown in FIG. 11 is used in the detection kit.

14 is a perspective view showing another state when the cuticle shown in FIG. 11 is used in the detection kit.

Fig. 15 is a perspective view showing a kit for detecting cuticle acquisition according to a fifth embodiment of the present invention.

FIG. 16 is a schematic diagram for explaining how to use the cuticle layer detection kit shown in FIG. 15.

FIG. 17 is a perspective view showing a state when the cuticle shown in FIG. 15 is used in the detection kit.

Fig. 18 is a perspective view showing another state when the cuticle shown in Fig. 15 is used in the detection kit.

Fig. 19 is a perspective view showing a cuticle collection kit according to a sixth embodiment of the present invention.

FIG. 20 is a perspective view showing a part of the cuticle collection detection kit shown in FIG. 19.

FIG. 21 is a perspective view showing a state where the cuticle shown in FIG. 19 is used in the detection kit.

FIG. 22 is a perspective view showing another state when the cuticle shown in FIG. 19 is used in the detection kit.

FIG. 23 is a perspective view showing still another state when the cuticle shown in FIG. 19 is used in the detection kit.

Hereinafter, an embodiment of the present invention will be described in detail with reference to the drawings.

"First Embodiment"

FIG. 1 is a perspective view showing a set 1 for horny layer acquisition detection according to a first embodiment of the present invention. The cuticle collection detection kit 1 includes: a cuticle extraction tool 10 for taking the cuticle from the skin of the subject; and a solvent container 20 containing a specific component in the cuticle taken by extraction Solvent (extract). Except for the adhesive layer 16 described below, the stratum corneum collection tool 10 and the solvent container 20 are basically made of, for example, synthetic resin, such as polystyrene, polypropylene, polyethylene, and ABS resin (Acrylonitrile-Butadiene-Styrene Resin, acrylic Nitrile-butadiene-styrene resin) and the like are formed by molding, but may be formed of other materials.

The stratum corneum harvesting tool 10 has a cylindrical rod-shaped portion 11 as an example, and a grip portion 12 gripped by a user is attached to one end portion thereof. The grip 12 includes a plug 13 and a cover 14. The plug portion 13 is formed in a substantially bottomed cylindrical shape, and the lower side, that is, the rod-shaped portion 11 side is opened, and on the other hand, the opposite side (upper portion) becomes the upper bottom and is closed. The cover 14 is held by the plug portion 13 via its hinge portion 14a so as to freely rock about the rocking axis C. The lower end of the cover 14 and the upper end of the plug 13 are configured, for example, by a locking groove formed in one of them and a locking convex portion (neither shown) formed in the other, thereby maintaining The cover 14 covers the state of the plug 13. In addition, the upper and lower portions of the plug portion 13 are covered and covered by the cover 14 in FIG. 1, but the structure of the upper and lower portions of the plug portion 13 will be described in detail later. In addition, on the inner peripheral surface of the cylindrical plug portion 13, a female thread (not shown) that is screwed into the following male thread 20b formed in the solvent container 20 is formed. In addition, a columnar portion 15 is formed. The columnar portion 15 is set in a state connected to the other end of the rod-shaped portion 11 and has a cylindrical shape with a slightly larger diameter than the rod-shaped portion 11. Furthermore, an adhesive layer 16 is formed on the entire peripheral surface of the columnar portion 15.

The above-mentioned adhesive layer 16 is formed by directly applying an adhesive agent to the outer peripheral surface of the columnar portion 15 or applying an adhesive agent to a sheet-shaped support and attaching it to the outer peripheral surface of the columnar portion 15. Specific examples of the adhesive include natural rubber, polyisobutylene rubber, polybutadiene rubber, silicone rubber, polyisoprene rubber, and styrene-isoprene-benzene. Synthetic rubber such as ethylene block copolymer rubber, synthetic resin such as polypropylene resin, polystyrene resin, polyacrylate copolymer resin, etc. In addition, as the adhesion-imparting agent, rosin, hydrogenated rosin and its ester, polyterpene resin, petroleum resin, etc. can also be used. In addition, specific examples of the support include paper, staple fiber, cotton, cloth, non-woven fabric, polyester resin, urethane resin, polyethylene resin, ethylene-vinyl acetate copolymer, and soft poly Films of various resins such as vinyl chloride, aluminum foil, etc., alone or in combination, but adhesives, adhesion-imparting agents, and supports are not limited to these.

On the other hand, as shown in more detail in FIG. 2, the solvent container 20 is an elongated bottomed cylindrical container having an opening 20 a in the upper portion. A male screw 20b is formed on the peripheral surface of the solvent container 20 in the vicinity of the upper end near the opening 20a. In addition, a portion near the center in the height direction of the solvent container 20 is a tapered portion 20c. The portion below the tapered portion 20c, that is, the portion that accommodates the columnar portion 15 of the stratum corneum harvesting tool 10 when the stratum corneum harvesting tool 10 is incorporated into the solvent container 20 is set to The diameter is smaller than the upper part of the tapered portion 20c. That is, if the cross-sectional area in the plane of the internal space of the solvent container 20 orthogonal to the cylinder axis direction is considered, it becomes a cross-section that receives the columnar portion 15 compared to the portion where the opening 20a is formed. The area is smaller.

The solvent container 20 contains a solvent inside. This solvent extracts the specific components in the stratum corneum that is taken to the stratum corneum with the adhesive layer 16 of 10 as described below. Furthermore, a temporary plug 21 for preventing the outflow of the solvent is attached to the solvent container 20. The temporary plug 21 is a substantially bottomed cylindrical member that is opened on the lower side and closed on the other side, and the upper side becomes the upper bottom and is closed, similar to the plug portion 13 of the stratum corneum extraction tool 10 described above. The temporary plug 21 is attached to the solvent container 20 by screwing a female thread (not shown) formed on the inner peripheral surface thereof to the male thread 20b.

In addition, in general, the above-mentioned cuticle collecting tool 10 and the solvent container 20 are packaged in a packaging bag (not shown) or the like for sale.

Next, a method of using the cuticle collection kit 1 of the present embodiment will be described. During the examination, the stratum corneum harvesting tool 10 is as shown in FIG. M grasps the grip 12 and operates it. In addition, the stratum corneum adopts the adhesive layer 16 of the system 10 to press against the skin of the test subject part of the subject H, that is, for example, the skin of the cheek. In this state, the grip 12 is rotated, even if the adhesive layer 16 is rotated, With this, the skin of the subject H is peeled off and the stratum corneum is taken over the wide range of the adhesive layer 16. Furthermore, it is also possible to wash the surface layer of the skin to remove sebum, dirt, cosmetics, etc. before taking the stratum corneum, but it is not necessary.

Once the stratum corneum is taken, the temporary plug 21 is removed from the solvent container 20 as shown by the one-dot chain line A in FIG. 2 and the opening 20a is exposed. This removal is performed by rotating the temporary bolt 21 to release the screwing with the male thread 20b. Furthermore, the temporary plug 21 can also be removed before the stratum corneum is taken.

As shown by a single dotted line B in FIG. 2, the stratum corneum extracting tool 10 that collects and holds the stratum corneum from the adhesive layer 16 is accommodated in the solvent container 20 from the columnar portion 15 through the opening 20a. Then, the female thread formed on the inner peripheral surface of the plug portion 13 is screwed with the male thread 20 b formed on the solvent container 20 to integrate the stratum corneum harvesting tool 10 and the solvent container 20.

FIG. 4 shows the state in which the stratum corneum collection tool 10 is accommodated in the solvent container 20. In this state, the stratum corneum adopts the adhesive layer 16 of the tool 10 and is immersed in the solvent contained in the solvent container 20. In this state, the opening 20a of the solvent container 20 is closed by the plug portion 13 and the lid 14 of the stratum corneum extraction tool 10. That is, the stratum 13 of the stratum corneum 10 and the cap 13 and the cover 14 constituting the grip 12 also function as a cover that closes the opening 20a.

In addition, when the cuticle extractor 10 and the solvent container 20 are integrated by screwing the female thread and the male thread, the cuticle extractor 10 and the solvent container 20 are coaxial, and the adhesive layer 16 is not in contact with the solvent container 20 The inner wall of the solvent container 20 is kept in contact with the inner wall. That is, when the plug portion 13 having the above-mentioned female thread and the male thread 20b of the solvent container 20 are inserted in the solvent container 20 into the stratum corneum collection tool 10, they are guided so that the adhesive layer 16 does not contact the inner wall of the solvent container 20 The stratum corneum adopts a guiding mechanism with 10 to function.

Furthermore, it is preferable that the amount of the solvent contained in the solvent container 20 is set to the amount where the liquid surface of the solvent is located near the upper end of the adhesive layer 16 when the stratum corneum taking tool 10 is contained in the solvent container 20, by which In this way, the amount of solvent can be suppressed to the minimum required amount. In addition, as long as the cuticle layer 10 is adjusted so that the gap between the inner peripheral surface of the solvent container 20 and the adhesive layer 16 becomes relatively small, the specific components of the cuticle can be extracted into the solvent at a high concentration, so Better. Here, the solvent liquid level may be higher or lower than the upper end of the adhesive layer 16. In addition, specifically, the amount of the solvent is, for example, about 1 mL (milliliter).

By accommodating the stratum corneum 10 in the solvent container 20, the adhesive layer 16 is pre-immersed in a solvent maintained at a specific temperature for a specific time, and the specific components of the stratum corneum taken and maintained by the adhesive layer 16 are extracted into the solvent in. In addition, as this solvent, for example, as shown in the above-mentioned Patent Documents 1 and 2, the expression of squamous cell carcinoma-associated antigen (SCCA) of skin keratinocytes is measured, and the skin of the subject H is determined at a biochemical level In case of sensitivity, reactivity, and sensitivity, Tris-buffer can be used.

By immersing the adhesive layer 16 in the solvent for a certain period of time as described above, the plug 14 is opened as shown in FIG. 5, and the solvent S is taken out from the solvent container 20. That is, a solvent extraction port 13a is formed at the bottom of the cylindrical plug 13 with a bottom, and the solvent S flows out from the solvent extraction port 13a. In addition, the solvent take-out port 13a can maintain the closed state by covering the cover 14 with the plug portion 13, and only open the cover when the cover 14 is opened as shown in FIG.

Here, in particular, the cap 14 and the solvent extraction port 13a may not be provided. When the solvent S is taken out, the plug 13 is taken out from the solvent container 20, and then the solvent S flows out from the exposed opening 20a.

The solvent S taken out of the solvent container 20 is supplied to a measuring device that measures the extracted SCCA. The measurement of SCCA can be performed quantitatively or qualitatively by any known method. Specific examples include immunoassay methods that use specific antibodies for SCCA, for example, ELISA (Enzyme Linked Immunosorbent Assay) method using enzyme labeling, and RIA (radioimmunoassay, radioactivity) using radiolabeling. Sexual immunoassay) method, immunoturbidimetric method, Western blot method, latex agglutination method, red blood cell agglutination method and other methods. The method of immunoassay may include a competition method or a sandwich method.

In the set 1 for detecting the stratum corneum of this embodiment, as described above, the amount of the solvent in the solvent container 20 is limited to the minimum amount required. Therefore, the specific component of the stratum corneum that is the object of detection and measurement is extracted into the solvent at a relatively high concentration, whereby the detection and measurement of the specific component are performed with high accuracy.

In addition, in the horny layer collection detection kit 1 of the present embodiment, as described above, the state is set as follows: compared to the cross-sectional area of the portion where the opening 20a is formed in the solvent container 20, the columnar portion 15 is received The cross-sectional area of the part is smaller. As a result, the volume of the portion that receives the columnar portion 15 becomes relatively small, and therefore it is advantageous in setting the amount of the solvent preliminarily contained in the portion to the minimum amount necessary for the adhesive layer 16 to be impregnated. On the other hand, since the portion where the opening 20a is formed has a relatively large diameter, the outer diameter of the grip portion 12 combined with this portion also becomes relatively large, and the grip portion 12 becomes easy to grip.

In the set 1 for detecting the stratum corneum collection according to the present embodiment, the columnar portion 15 has a larger diameter than the rod-shaped portion 11, but it may be constituted by one member having a uniform diameter. In addition to the cylindrical shape, the rod-shaped portion 11 and the cylindrical portion 15 may be formed into, for example, a rectangular columnar shape or a hexagonal columnar shape, and these shapes are further hollow in the inside (for example, a round tube or a square tube). .

In addition, in order to make the volume of the portion of the solvent container 20 that receives the columnar portion 15 relatively small, the wall thickness of this portion may be increased, and the tapered portion 20c is not provided. However, in this case, since the wall thickness of the portion of the solvent container 20 that receives the columnar portion 15 is unnecessarily increased, the usage amount of, for example, synthetic resin forming the solvent container 20 is undesirably increased, which is not preferable.

The cuticle layer detection kit of the present invention can also be constructed as a kit for measuring the abundance of factors related to other arbitrary skin traits, not limited to the above SCCA measurement. For example, as shown in Patent Document 3 above, it can be used as a measure of nerve growth in the stratum corneum It consists of abundance of sub- and/or neurotrophic factor 4. In this case, as a solvent for extracting nerve growth factor or nerve nourishing factor 4, a conventional physiological buffer solution can be used. Particularly preferred are applications such as Dulbeco's phosphate buffered saline, etc., but not limited thereto.

"Second Embodiment"

Next, a second embodiment of the present invention will be described with reference to FIG. 6. In addition, in FIG. 6, the components equivalent to those already described are denoted by the same reference numbers, and descriptions of the components are omitted as long as there is no special need (the same applies hereinafter).

The second embodiment of the stratum corneum collection detection kit 2 is different from that of the stratum corneum collection detection kit 1 shown in FIGS. 1 and 2 in the structure of the lid that closes the opening 20a of the solvent container 20. That is, in the set 2 for horny layer extraction detection, a convex solvent outflow portion 13b is formed on the bottom portion of the plug portion 13 of the horny layer extraction device 10, and is provided inside the solvent outflow portion 13b. The solvent extraction port 13a with an open end. The solvent outflow portion 13b is covered with a lid 17, and the solvent outlet 13a is closed by the lid 17. Furthermore, a male thread is formed on the outer peripheral surface of the solvent outflow portion 13b, and on the other hand, a female thread is formed on the inner peripheral surface of the cover 17, and the two threads are screwed together, whereby a cover is provided on the solvent outflow portion 13b 17.

In the case of using the cuticle collection kit 2 having the above configuration, the use of the cuticle acquisition device 10 to take the cuticle and the cuticle acquisition device 10 to be accommodated in the solvent container 20 are also the first implementation. The horny layer of the form is the same as when the test kit 1 is used. After the adhesive layer 16 is immersed in the solvent contained in the solvent container 20, when the solvent is taken out from the solvent container 20, the cap 17 is rotated to loosen the screwing of the two threads, and the cap 17 is removed from the solvent outflow portion 13b. As a result, the solvent extraction port 13a is in a state where the plug is opened, and the solvent is taken out of the solvent container 20 from there.

"Third Embodiment"

Next, a third embodiment of the present invention will be described with reference to FIGS. 7 to 10. The third embodiment of the stratum corneum collection kit for detection 3 and the stratum corneum collection shown in FIGS. 1 and 2 Compared with the test kit 1, the basic difference is that a cover body that closes the opening 20 a of the solvent container 20 after immersing the adhesive layer 16 in the solvent is formed on the solvent container 20 side.

That is, as shown in FIG. 7, in the stratum corneum sampling device 10 constituting the stratum corneum collection detection kit 3, on one end side of the rod-shaped portion 11, that is, on the side opposite to the columnar portion 15, there is installed The plug portion 13 shown in FIG. 1 is also formed as a bottomed cylindrical grip portion 30. When the stratum corneum harvesting tool 10 is used to pick up the stratum corneum, the grasping portion 30 is gripped by a human hand to operate the stratum corneum harvesting tool 10.

On the other hand, in the solvent container 20, a plug portion 31 formed into a bottomed cylindrical shape like the temporary plug 21 shown in FIG. 1 is attached. The plug portion 31 is attached to the upper portion of the solvent container 20 by screwing a female thread (not shown) formed on its inner peripheral surface with a male thread 20b shown in FIG. 8. In addition, a cover 32 that constitutes the cover body together with the plug portion 31 is attached to the plug portion 31. The lower end of the cover 32 and the upper end of the plug 31 are configured, for example, in such a manner that the locking groove formed in one of them and the locking convex portion (both not shown) formed in the other are locked, whereby The state in which the cover 32 covers the plug portion 31 is maintained. In addition, a part of the outer peripheral surface of the plug part 31 is provided with a lock release part 31a which assists the release of the lock of the plug part 31 and the cover 32 by bending when pressed.

Hereinafter, a method of using the cuticle collection kit 3 of the present embodiment will be described. In the case of using the cuticle to take the detection kit 3, the use of the cuticle to take the horny layer 10 and the cuticle to take the horn 10 to be accommodated in the solvent container 20 are also the same as using the cuticle of the first embodiment The same applies to the case where the layer takes the detection kit 1. In addition, before storing the stratum corneum harvesting tool 10 in the solvent container 20, as shown by the single-dot chain line A in FIG. 8, the plug portion 31 is removed from the upper portion of the solvent container 20. Also, as shown by the single-dot chain line B in FIG. 8, the stratum corneum collection tool 10 is accommodated in the solvent container 20 from the columnar portion 15 through the opening 20 a. The state at this time is shown in Fig. 9.

If the adhesive layer 16 is immersed in the solvent in the solvent container 20 for a certain period of time as described above, the specific components of the stratum corneum taken and maintained by the adhesive layer 16 are extracted to In the solvent, the stratum corneum extractor 10 is taken out from the solvent container 20. Then, the plug part 31 is attached to the upper part of the solvent container 20 again, and the opening 20a is closed.

Then, as shown in FIG. 10, the locking of the plug portion 31 and the cover 32 is released, and the cover 32 is removed from the plug portion 31. At the top and bottom of the plug portion 31, a solvent extraction port 31b is opened, and when the cover 32 is removed, the solvent extraction port 31b becomes a plug-opening state. In this state, if the solvent container 20 is in a posture with its upper part facing down, the solvent S containing the extracted specific components of the stratum corneum is taken out of the solvent container 20 from the solvent extraction port 31b.

Furthermore, in the kit for detecting the stratum corneum of the present invention, the solvent container 20 may be formed of a relatively high rigidity material, or may be formed of a relatively low rigidity and flexible material. In the case where the solvent container 20 is formed of a material like the latter, even if the solvent extraction port 31b is small and the solvent S is difficult to flow out from there, the outer surface of the solvent container 20 can still be gently pressed to deform it, thereby making The solvent S flows out smoothly.

"Fourth Embodiment"

Next, a fourth embodiment of the present invention will be described with reference to FIGS. 11 to 14. Compared with the cuticle layer detection kit 2 shown in FIG. 6 in the fourth embodiment, the cuticle layer detection kit 4 is basically different in that the solvent container 20 is immersed in the solvent after the adhesive layer 16 is immersed in the solvent. A lid whose opening 20a is closed is formed on the solvent container 20 side.

That is, as shown in FIG. 11, on the horny layer sampling device 10 constituting the horny layer collection detection kit 4, on one end side of the rod-shaped portion 11, that is, on the side opposite to the column-shaped portion 15, a The plug portion 13 shown in FIG. 1 is also formed as a bottomed cylindrical grip portion 30. When the stratum corneum harvesting tool 10 is used to pick up the stratum corneum, the grasping portion 30 is gripped by a human hand to operate the stratum corneum harvesting tool 10.

On the other hand, a plug portion 40 formed in a cylindrical shape with a bottom is attached to the solvent container 20. The plug portion 40 is attached to the upper portion of the solvent container 20 by screwing a female thread (not shown) formed on its inner peripheral surface with a male thread 20b shown in FIG. 12. A convex solvent outflow portion 40b is formed on the upper and lower portions of the plug portion 40, and inside the solvent outflow portion 40b is provided at the upper end The face opening solvent extraction port 40a. The solvent outflow portion 40b is covered with a cover 42, and the cover 42 is used to close the solvent extraction port 40a. In addition, a male thread is formed on the outer peripheral surface of the solvent outflow portion 40b, and on the other hand, a female thread is formed on the inner peripheral surface of the cover 42 to screw the two threads together, thereby covering the solvent outflow portion 40b with盖42。 42.

Hereinafter, a method of using the cuticle layer detection kit 4 of the present embodiment will be described. In the case of using the cuticle to take the test kit 4, the use of the cuticle to take the cuticle 10 and the cuticle to take the 10 in the solvent container 20 are also the same as using the cuticle of the first embodiment The same applies to the case where the layer takes the detection kit 1. In addition, before storing the stratum corneum harvesting tool 10 in the solvent container 20, the plug 40 is removed from the upper portion of the solvent container 20 as indicated by the single-dot chain line A in FIG. Also, as shown by the single-dot chain line B in FIG. 12, the stratum corneum taking tool 10 passes through the opening 20 a from the columnar portion 15 and is accommodated in the solvent container 20. The state at this time is shown in Fig. 13.

If the adhesive layer 16 is immersed in the solvent in the solvent container 20 for a certain period of time as described above, and the specific components of the stratum corneum taken and maintained by the adhesive layer 16 have been extracted into the solvent, it is taken out from the solvent container 20 The cuticle takes 10. Then, the plug 40 is attached to the upper part of the solvent container 20 again, and the opening 20a is closed.

Then, as shown in FIG. 14, the screwing of the solvent outflow portion 40 b and the cover 42 is released, and the cover 42 is removed from the plug portion 40. When the cover 42 is removed in this way, the solvent extraction port 40a is in the unblocked state. In this state, if the solvent container 20 is in a posture with its upper part facing down, the solvent S containing the extracted specific components of the stratum corneum is taken out of the solvent container 20 from the solvent extraction port 40a.

"Fifth Embodiment"

Next, a fifth embodiment of the present invention will be described with reference to FIGS. 15 to 18. The 5th embodiment of the stratum corneum collection detection kit 5 is compared with the stratum corneum collection detection kit 3 shown in FIG. 7 to FIG. 10, and the basic difference is that:持部30。 30 holding section. That is, in this embodiment, one end of the rod-shaped portion 11 itself is provided To grasp the stratum corneum, the grasping portion of the tool 10 is used.

The method of using the cuticle layer detection kit 5 of the present embodiment will be described below. In the case of using the cuticle to take the set 5 for detection, the use of the cuticle to take the cuticle 10 is taken, and the cuticle is taken from the solvent container 20 and the third embodiment of the cuticle The case where the layer adopts the detection kit 3 is basically the same. However, in this case, as described above, the grasping of the stratum corneum 10 is performed by grasping one end of the rod-shaped portion 11.

Before accommodating the stratum corneum harvesting tool 10 in the solvent container 20, the plug portion 31 is removed from the upper portion of the solvent container 20 as shown by the single-dot chain line A in FIG. Also, as shown by the one-dot chain line B in FIG. 16, the stratum corneum collecting tool 10 is accommodated in the solvent container 20 from the columnar portion 15 through the opening 20 a. Thereafter, the plug portion 31 is attached to the upper portion of the solvent container 20 again, and the opening 20a is closed. The state at this time is shown in Fig. 17. Furthermore, inside the plug portion 31, a guide member (guide mechanism) loosely fitted with the rod-like portion 11 is formed. By this fitting, the stratum corneum taking tool 10 and the solvent container 20 are coaxial and provided The adhesive layer 16 is held in the solvent container 20 without being in contact with the inner wall of the solvent container 20.

In the manner described above, the adhesive layer 16 is immersed in the solvent in the solvent container 20 for a specific time, and the specific components of the stratum corneum taken and maintained by the adhesive layer 16 are extracted into the solvent. Then, as shown in FIG. 18, the locking of the plug portion 31 and the cover 32 is released, and the cover 32 is removed from the plug portion 31. At the top and bottom of the plug portion 31, a solvent extraction port 31b is opened, and when the cover 32 is removed, the solvent extraction port 31b becomes a plug-opening state. In this state, if the solvent container 20 is in a posture with its upper part facing down, the solvent S containing the extracted specific components of the stratum corneum is taken out of the solvent container 20 from the solvent extraction port 31b.

"Sixth Embodiment"

Next, a sixth embodiment of the present invention will be described with reference to FIGS. 19 to 23. As shown in FIG. 19, the horny layer collection kit 6 of the sixth embodiment includes the same horny layer collection tool 10 as shown in FIG. 15, and a relatively thin bottomed cylindrical solvent container 器50.

As shown in FIG. 20, the solvent container 50 also has a flange portion 51 at the upper end. The flange portion 51 has a hinge portion 51a protruding laterally and an opening 51b. The above-mentioned opening 51b of the flange portion 51 functions as an opening of the solvent container 50 for receiving the cuticle. In addition, the solvent container 50 including the flange portion 51 and the lid 52 described below are formed of, for example, synthetic resin.

A cover 52 is disposed on the opening 51b. The cover 52 has a hinge portion 52a protruding laterally, and the hinge portion 52a and the hinge portion 51a are hinged by bending by a synthetic resin. Thereby, the lid body 52 can obtain the lid closing position where the opening 51b is closed as shown in FIG. 19, and the lid opening position where the opening 51b is exposed as shown in FIG. 20.

A solvent outflow portion 52b is formed on the upper surface of the lid 52. The solvent outflow part 52b has the following solvent extraction port 52e (refer to FIGS. 22 and 23), and one end of the solvent extraction port 52e passes through a communication hole (not shown) formed in the cover 52 and is inside the cover 52 Surface opening. In addition, the other end of the solvent extraction port 52e is closed by a peeling piece 52c integrally formed with the solvent outflow portion 52b before the cuticle layer collection kit 6 is used. A cylindrical portion 52d is formed on the inner surface of the lid body 52. When the lid body 52 is set to the above-mentioned closing position, the cylindrical portion 52d is fitted into the opening 51b substantially tightly.

Hereinafter, a method of using the cuticle collection kit 6 of the present embodiment will be described. In the case of using the cuticle to take the set 6 for detection, the use of the cuticle to take the cuticle 10 is also carried out in the same way as when the cuticle shown in FIG. 15 is used to take the set of 5 for detection .

The stratum corneum picking tool 10 that picks up and holds the stratum corneum in the adhesive layer 16 is housed in the solvent container 50 with the lid 52 set to the lid opening position as shown in FIG. 20 through the opening 51b. When the stratum corneum harvesting tool 10 is accommodated in the solvent container 50, the lid 52 is set to the lid closing position, and the opening 51b is closed. The state at this time is shown in FIG. 21. In this way, the solvent in the solvent container 50 is impregnated with the adhesive layer 16 that takes and holds the stratum corneum. Furthermore, in the cylindrical portion 52d Inside, a guide member (guide mechanism) loosely fitted with the rod-shaped portion 11 is formed, and by this fitting, the stratum corneum taking tool 10 is coaxial with the solvent container 50, and the adhesive layer 16 is not in contact with the solvent The inner wall of the container 50 is held in the solvent container 50 in a state of contact.

When the adhesive layer 16 is immersed in the solvent for a certain period of time, as shown in FIG. 22, the peeling sheet 52c is peeled from the solvent outflow portion 52b. As a result, the solvent extraction port 52e formed in the solvent outflow portion 52b is in a state of being opened. Furthermore, the solvent outflow portion 52b is formed at a position deviated from the rod-shaped portion 11 held in the solvent container 50.

Then, as shown in FIG. 23, if the upper part of the solvent container 50 is directed downward, the solvent S containing the extracted specific components of the stratum corneum is taken out of the solvent container 50 from the solvent extraction port 52e.

In addition, the horny layer sampling device 10 can be used alone for the horny layer sampling application in addition to the above-described solvent container 20 or 50 to constitute the horny layer sampling detection kit. If such a stratum corneum harvesting tool 10 is used, the stratum corneum can be harvested in a simpler operation than the well-known tape peeling method or the like.

10‧‧‧ cuticle

11‧‧‧Bar-shaped part

13‧‧‧bolt

13a‧‧‧Solvent export

14‧‧‧ cover

14a‧‧‧Hinge Department

15‧‧‧Columnar part

16‧‧‧adhesive layer

20‧‧‧Solvent container

20c‧‧‧taper

S‧‧‧Solvent

Claims (11)

A cuticle collection detection kit, comprising: a cuticle collection tool, which includes a rod-shaped portion having a grip portion gripped by a user at one end, and a cylindrical portion connected to the other end of the rod-shaped portion , And an adhesive layer formed on the peripheral surface of the columnar portion; a solvent container, which is a bottomed cylindrical container with an opening in the upper part, and contains the solvent to be extracted into the horny layer of the adhesive layer , The columnar portion containing the cuticle taken from the opening in such a way that the adhesive layer can be immersed in the solvent; and a lid body, which immerses the adhesive layer in the solvent, closes the opening of the solvent container; and Furthermore, it has a solvent extraction port formed in the solvent container or the lid so as to maintain a closed state, and the cap is opened to take the solvent out of the solvent container out of the solvent container. If the cuticle of claim 1 is a test kit, wherein the cross-sectional area of the internal space of the solvent container in the plane orthogonal to the direction of the cylinder axis is set as compared to the cross-sectional area of the portion where the opening is formed , The cross-sectional area of the part that accepts the columnar part is smaller. If the cuticle of claim 1 is a detection kit, the amount of the solvent is set such that the liquid surface of the solvent is located near the upper end of the adhesive layer immersed in the solvent. If the cuticle of claim 2 is a detection kit, the amount of the solvent is set such that the liquid surface of the solvent is located near the upper end of the adhesive layer immersed in the solvent. If the stratum corneum of claim 1 adopts a kit for detection, the above-mentioned gripping part also serves as the above Cover body. If the cuticle of claim 2 adopts a kit for detection, the gripping part also serves as the cover. If the cuticle of claim 3 is a detection kit, the gripping part also serves as the lid. If the horny layer of claim 1 is a detection kit, which has a guide mechanism, the guide mechanism takes the horny layer inserted into the solvent container in such a manner that the adhesive layer does not contact the inner wall of the solvent container Positioning. If the horny layer of claim 2 is a detection kit, which has a guide mechanism, the guide mechanism takes the horny layer inserted into the solvent container in such a manner that the adhesive layer does not contact the inner wall of the solvent container Positioning. If the horny layer of claim 3 is a detection kit, it has a guide mechanism, which guides the horny layer inserted into the solvent container in such a way that the adhesive layer does not contact the inner wall of the solvent container Positioning. If the horny layer of claim 4 is a detection kit, it has a guide mechanism, which guides the horny layer inserted into the solvent container in such a manner that the adhesive layer does not contact the inner wall of the solvent container Positioning.

Images