TWI675673B - Biofilm removal system - Google Patents

Abstract

The invention discloses a biofilm removal system, which is applied to remove a biofilm in a cavity. The biofilm removal system includes a carrier and a removal device. The carrier includes nanometer magnetic beads, bacteriostatic substance, and an outer film covering the bacteriostatic substance and nanometer magnetic beads. The cleaning device includes a magnetron component and a vibration component. The magnetic control component is used to guide the nanometer magnetic beads to drive the carrier to approach the biofilm. The vibration component is used to generate vibration and focus on the biofilm, while generating micro-bubbles makes at least a part of the biofilm detach from the inner cavity wall, and destroys the outer membrane to release bacteriostatic substances. The invention uses a vibration component to peel off most of the biofilm, and at the same time causes the carrier to release a bacteriostatic substance to suppress the remaining bacteria, thereby achieving high-efficiency and precise treatment.

Description

Biofilm removal system

A system for removing biofilm, especially a system for removing biofilm in cavity using vibration and magnetic force.

With the advancement of medicine, more and more tubing and artificial implants are being used for medical treatment and improving human life. However, while receiving the benefits of these medical aids, the problem of infection is an unavoidable and difficult problem. In particular, bacterial infections often cause complications and sequelae, and even serious enough to cause sepsis. Therefore, the prevention and treatment of bacterial infections is the research direction of current medical research. In particular, urinary tract infections account for about 40% of medically related infections. And each time the urinary catheter is left in the body, the incidence of bacteriuria will increase by 3% to 10%. Therefore, the effective removal of bacterial infections in the bladder is a problem that must be improved at present.

To solve the problem of bacterial infection, the formation of biofilms must be solved first, because the formation of biofilms is a survival strategy for microorganisms. A biofilm is a structure formed by the attachment of microorganisms and extracellular substances secreted by microorganisms to the surface of an object. In other words, a biofilm is a community of microorganisms. The main purpose of group growth is to resist the pressure brought by the external environment, such as: nutritional deficiency, pH value, extreme temperature, or even the threat of detergents or antibiotics.

For the removal of biofilms formed in the human body, the current medical methods are mostly physical treatments such as direct vortexing, scraping, rinsing, and scrubbing of biofilms or antibiotics or Biochemical treatment of medicaments. However, the consensus in antibiotic treatment is that the lower the dose, the better, if necessary, to reduce the risk of bacteria developing resistance. More importantly, it is very easy to damage normal tissues and cells when removing biofilms using conventional methods. Therefore, there is an urgent need for a technology that can implement precision medicine and inhibit biofilm formation.

In view of this, the present invention discloses a biofilm removal system, which is applied to remove a biofilm on an inner cavity wall of a cavity. Liquid is also contained in the cavity. The biofilm removal system includes a carrier and a removal device. The carrier is located in the cavity and contains a nano-magnetic bead, a bacteriostatic substance for targeted inhibition of the biofilm, and a heat-sensitive lipid film covering the bacteriostatic substance and the nano-magnetic bead. The removal device is located outside the cavity and includes a magnetron component and a vibration component. The magnetic control component is used to guide the nanometer magnetic beads to drive the carrier to approach the biofilm. The vibration component is used to generate a plurality of vibrations and focus the vibrations within a defined distance of the biofilm or a biofilm, so as to generate micro-bubbles in the liquid to cause at least a part of the biofilm to escape from the inner cavity wall and damage the thermal sensitivity Lipid film to release bacteriostatic substances.

The vibration assembly further includes a plurality of vibration elements. The vibration elements are arranged to form a phased array. Each vibrating element sequentially generates vibration and the energy of the vibration reaches the biofilm or a limited distance outside the biofilm at the same time.

The vibration component further focuses the vibration into the cavity to cause the liquid to vortex or turbulence, and at least a part of the biofilm is detached from the inner cavity wall by the vortex or turbulence.

The pressure wave generated when the micro-bubbles generated by the vibration component in the cavity is destroyed acts on the biofilm, so that at least a part of the biofilm is separated from the inner cavity wall.

The frequency of the vibration generated by the vibration component is between 0.3 MHz and 4.0 MHz.

The magnetron component further generates a high-frequency AC magnetic field to generate thermal energy to suppress the biofilm and destroy the heat-sensitive lipid film to release bacteriostatic substances.

The removal device further includes a first probe, and the first probe includes the aforementioned magnetron component, vibration component, and a magnetic shield. The magnetic shielding is located between the magnetron component and the vibration component to reduce the electromagnetic effect of the magnetron component on the vibration component.

The carrier is approximately circular, and the diameter of the carrier is less than 0.22 μm.

The carrier further includes a biofilm ligand. The first end of the biofilm ligand is joined to the outside of the thermosensitive lipid film, and the second end of the biofilm ligand is used to join the biofilm.

The biofilm removal system further includes a second probe for receiving an echo, which is generated by the vibration generated by the vibration component or a second vibration generated by a second vibration source touching the cavity and the biofilm.

The biofilm removal system of the present invention provides multiple removal methods, and different effects can be obtained by performing these removal methods separately or simultaneously.

One of the methods to remove the biofilm in the cavity is to inject the carrier into the cavity; the nanomagnetic beads of the magnetic control component are used to guide the nanomagnetic beads to drive the carrier to approach the biofilm; the vibration component is controlled to generate a plurality of vibrations, and the vibrations are focused Within a defined distance from the biofilm or one outside the biofilm; use these vibrations to focus to generate multiple microbubbles that contact the biofilm, so that at least a part of the biofilm is detached from the inner cavity wall; at the same time, the energy of the vibration or the thermal energy generated by the vibration is destroyed The heat-sensitive lipid film releases the bacteriostatic substance. Since the carrier is close to the biofilm, the bacteriostatic substance will also smoothly affect the bacteria in the biofilm.

Another method to remove the biofilm in the cavity is to inject a carrier into the cavity; use the biofilm ligand on the carrier to specifically bind to the biofilm; control the production of the magnetron component The generation of high-frequency AC magnetic field causes the nanometer magnetic beads in the carrier to generate magnetic vibration; the magnetic vibration generates heat energy higher than the biological body temperature to cause the bacteria in the biofilm to die; the thermosensitive lipid film of the carrier is caused by the magnetic vibration and the thermal energy generated by the magnetic vibration Destroyed, releasing bacteriostatic substances inside.

Another method for removing the biofilm in the cavity includes the following steps: close the vibration component to the corresponding outer surface of the cavity; control the vibration component to generate a plurality of vibrations and focus the vibrations on the biofilm; then, use these vibrations to focus and generate The plurality of micro-bubbles are contacted with the biofilm to disengage at least a part of the biofilm from the inner cavity wall.

Compared with the conventional technology, the method for removing the biofilm in the cavity of the present invention can be used to remove the biofilm in the body. The mechanism of using a vibration device with a phased array probe to peel off the biofilm can remove the biofilm deep into the inner cavity wall more than the surgical method using physical debridement. The method of using the vibration component with the phased array probe to peel off the biofilm is more harmless to the patient's body tissue than the current removal method. In the invention, the carrier and the nano-magnetic beads are used to carry the medicine to reach the biofilm site, so that the medicine can perform its functions more accurately. This method can reduce the dose of the drug, avoid damage to the tissue, and reduce the burden of the body's metabolic drugs. The invention can be performed directly from the outside of the patient who placed the urinary catheter, and the removed biofilm is discharged out of the body through the urinary catheter. The device can also enter the patient's bladder along the urinary catheter, and it is easier to grasp the focus position of vibration.

In addition, the vibration module is used to strip most of the obvious biofilms, but it cannot remove all bacteria in the space. Bacteria will still clump quickly after the operation and form a biofilm again. On the other hand, various drugs (such as antibiotics) cannot easily pass through the protection of the biofilm, and even if the dose is increased to the maximum, the bacteria in the biofilm cannot be completely removed. The system of the present invention further combines the advantages of the complementary two, peeling off most of the visible biofilm with the vibration component, and at the same time The carrier releases the agent, and most of the peeled biofilm can be removed by flowing out of the cavity through other methods, and the bacteria or free bacteria remaining in the small amount of biofilm remaining in the cavity will be eliminated by the agent. Therefore, the effect of removing the biofilm by the present invention is far superior to the existing biofilm cleaning technology.

1‧‧‧Biofilm removal system

2‧‧‧Clear device

3‧‧‧ carrier

4‧‧‧biofilm

5‧‧‧ Cavity

21‧‧‧first probe

23‧‧‧Magnetron assembly

24‧‧‧ Magnetic shielding

25‧‧‧Vibration components

30‧‧‧ heat sensitive lipid film

32‧‧‧ Bacteriostatic substance

37‧‧‧Biofilm ligands

38‧‧‧Nano Magnetic Beads

50‧‧‧ Inner cavity wall

51‧‧‧Liquid

251‧‧‧Vibration element

FIG. 1 is a schematic diagram of a cavity and a biofilm in the cavity according to a specific embodiment of the present invention.

FIG. 2 is a schematic diagram of a carrier in a specific embodiment of the present invention.

FIG. 3 is a schematic diagram of a cleaning device in a specific embodiment of the present invention.

FIG. 4 is a schematic diagram of a biofilm removal system in a specific embodiment of the present invention.

FIG. 5 is a schematic diagram of a cleaning device according to another embodiment of the present invention.

FIG. 6 is a schematic diagram of a carrier according to another embodiment of the present invention.

In order to make the advantages, spirits and features of the present invention easier and clearer, it will be detailed and discussed in the following with reference to the embodiments and the accompanying drawings. It is worth noting that these examples are only representative examples of the present invention. But it can be implemented in many different forms and is not limited to the embodiments described in this specification. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

The terms used in the various embodiments disclosed in the present invention are only used for describing the specific embodiments, and are not intended to limit the various embodiments disclosed in the present invention. As used herein, singular forms also include plural forms unless the context clearly indicates otherwise. Unless otherwise defined, all terms (including technical and scientific terms) used in this specification have the same meanings as commonly understood by one of ordinary skill in the art to which the various embodiments disclosed herein belong. The above terms (such as those defined in commonly used dictionaries) will be interpreted as having the same meaning as the contextual meaning in the same technical field, and will not be interpreted as having an idealized or overly formal meaning Unless clearly defined in the various embodiments disclosed herein.

In the description of this specification, the description of the reference terms “an embodiment”, “a specific embodiment” and the like means that specific features, structures, materials, or characteristics described in connection with the embodiment are included in at least one embodiment of the present invention. . In this specification, the schematic expressions of the above terms do not necessarily refer to the same embodiment. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments.

See Figures 1 to 4. FIG. 1 is a schematic diagram of a cavity 5 and a biofilm 4 therein according to a specific embodiment of the present invention. FIG. 2 is a schematic diagram of a carrier 3 in a specific embodiment of the present invention. FIG. 3 is a schematic diagram of a cleaning device 2 in a specific embodiment of the present invention. FIG. 4 is a schematic diagram of a biofilm removal system 1 in a specific embodiment of the present invention. The present invention discloses a biofilm removal system 1 for removing a biofilm 4 from an inner cavity wall 50 in a cavity 5. A liquid 51 is also contained in the cavity 5. The biofilm removal system 1 includes a carrier 3 and a removal device 2. The carrier 3 is located in the cavity 5 and includes a nanomagnetic bead 38, a bacteriostatic substance 32 for targeted inhibition of the biofilm 4, and a thermosensitive lipid film 30 covering the bacteriostatic substance 32 and the nanomagnetic bead 38. . The cleaning device 2 includes a magnetron component 23 and a vibration component 25. The magnetic control component 23 is used to guide the nano-magnetic beads 38 to drive the carrier 3 to approach the biofilm 4. The vibration component 25 is used to generate a plurality of vibrations and focus the vibrations within a defined distance of the biofilm 4 or one of the outside of the biofilm 4 so as to generate micro-bubbles in the liquid 51 to cause at least a part of the biofilm 4 to escape from the inner cavity wall. 50, and the thermosensitive lipid film 30 is destroyed to release the bacteriostatic substance 32.

In the present invention, two principles are used to remove the biofilm 4 in cooperation. One is physical removal, and the energy of the hole effect is used to peel the biofilm 4 from the inner cavity wall 50. Available later The opening of the cavity 5 naturally discharges the peeled biofilm. The other is biochemical removal. The magnetic field is used to drive the carrier 3 to the biofilm 4 and then release the antibacterial substance 32 in the biofilm 4. These two methods of removal have their own advantages and disadvantages. It is difficult to physically remove bacteria in tiny biofilms that are difficult to detect, and biochemical removal is difficult to poison bacteria in a wide range of biofilms. However, with the present invention, two removal methods can be performed in the same course of treatment, and both can use the vibration generated by the vibration component 25 to achieve removal, so that the effect of removing biofilm is more efficient than the traditional method.

The cavity 5 may refer to a bladder, a urethra, or other organs containing liquid and high humidity, and may also be a medical device for holding liquid, a medical device for transmitting liquid, and the like, but is not limited thereto. The biofilm 4 refers to a substrate produced by the breeding of bacteria, so that the bacteria produce a community therein. The heat-sensitive lipid film 30 is formed by heat-sensitive nano-liposomes. The bacteriostatic substance 32 can achieve universal or specific inhibition effects against different types of bacteria, thereby inhibiting biofilm formation or expansion, or directly killing bacteria. For example, antibiotics against Escherichia coli, radioactive substances, antibodies against antigens, compounds that generate heat energy when released from the carrier 3, vaccines against certain viruses or bacteria, alcohol, bactericides or disinfectants, etc. Effective compounds.

Among the elements contained in the carrier 3, the bacteriostatic substance 32 may also be replaced by an environment improving substance. In principle, the environment-improving substance does not directly inhibit bacteria, but it can change the internal environment of the cavity, enhance the immunity of the cavity, make it difficult for bacteria to grow, or prevent the formation of biofilms. The environment-improving substance can be a beneficial bacteria, which is relatively harmless to the organism, but the beneficial bacteria will change the environment of the cavity in which it is located. Such an environment is not suitable for the growth of some species of bacteria, thereby repelling harmful bacteria to the organism. The environment-improving substance can be an acidic compound or a basic compound. When the compound is released, it will change the pH value of the cavity, causing bacteria to die below this pH value, or it is not easy to reach this pH value. Growth inside the cavity. When the cavity system is an organ of an organism, the environment-improving substance can be a nutrient to make the organ get sufficient nutrition, thereby making the organ healthy and naturally improving immunity; when the organ or tissue is damaged by bacterial infection, the environment The improving substance can be a biological repair factor, which can improve organs and tissues and restore their original immunity.

In the present invention, the environment-improving substance and the bacteriostatic substance 32 can be reasonably replaced with each other or be contained in the carrier 3 at the same time.

The cavity 5 is not a sealed structure, or in other words, it is not a sealed structure at least in part, so that the carrier 3 can enter the cavity 5 and the peeled biofilm 4 can leave the cavity 5. The present invention can be applied to clear biofilm in the excretory system of the living body. At this time, the cavity 5 may be a bladder or a urethra. The carrier 3 is formed outside the cavity 5 and can be introduced into the cavity 5 by means of introduction, taking, injection, infusion, and the like. In a specific embodiment, the cavity 5 is a bladder, which is introduced into the bladder through a urinary catheter injection carrier 3 already installed on the patient. The removal device 2 does not need to invade the interior of the cavity 5. The removal device 2 is medically defined as a non-invasive treatment device. The stripped biofilm 4 is then discharged through the catheter. In still another embodiment, the removal device 2 can also enter the interior of the cavity 5 through an existing tube, for example, the inside of the bladder through a urinary catheter, thereby making it easier to control the focus position of the vibration during surgery.

The method for removing a biofilm in a cavity of the present invention can be used to remove a biofilm in a living body, or to remove a biofilm in a medical device. The mechanism of using the vibrating component to peel off the biofilm can remove the biofilm deep into the inner cavity wall more than the surgical method using physical debridement. The method of using the vibrating component to peel off the biofilm is less harmful to the patient's body tissue than the current removal method. In the invention, the carrier and the nano-magnetic beads are used to multiply the bacteriostatic substance to the location of the biofilm, so that the bacteriostatic substance can exert its function more accurately. By this method It can reduce the dose of bacteriostatic substances, thereby reducing the burden on the body's metabolism of bacteriostatic substances, and also reduce the degree of bacterial resistance. The invention can directly remove the removal device into the bladder along the catheter of the patient, or directly remove it from the outside of the body, and the removed biofilm is then discharged out of the body through the catheter.

The vibration assembly 25 further includes a plurality of vibration elements 251. The vibration elements 251 are aligned to form a phased array. Each vibration element 251 sequentially generates vibrations and the energy of the vibrations reaches the biofilm 4 or within a limited distance outside the biofilm 4 at the same time. The limited distance is 30mm. An effective operating range can be roughly divided by limiting the distance. The longest distance from the effective operating range to the biofilm 4 is the defined distance.

In a specific embodiment, the vibration component 25 is an ultrasonic vibration component, and the ultrasonic vibration component has a phased array of ultrasonic elements.

For practical applications, the vibration component 25 can use high-intensity focused ultrasound technology to achieve that the energy reaches the desired position at the same time. The principle is that the distance and angle between each vibration element 251 and the target position are different, but the transmission speed of the vibration wave is the same. When the vibrating element 251 far from the target position vibrates first, and the vibrating element 251 closer to the target position vibrates again within a very short time, two vibration energy will reach the target position at the same time, making the target position instantaneous Receiving high energy causes subsequent physical phenomena. The advantage of using this technology is that the material between the vibrating component 25 and the target location will not receive a momentarily high energy and will have a severe impact. This technology is used to remove tissues and organs between or around the biofilm and the vibration component 25 when the biofilm is removed from the body. On the other hand, it can reduce the energy absorbed by tissues and organs, and have enough energy to reach the biofilm of the target location. When the energy is focused in the biofilm 4, the vibration energy causes thermal energy in the biofilm. This thermal energy can exceed 40 degrees, destroying the physiology or structure of bacteria in the biofilm, thereby causing the bacteria to die. Or again In another embodiment, the energy of the vibration affects the chromosomes of the bacteria, causing the chromosomes to break and causing the bacteria to die.

In a specific embodiment, the present invention uses vibration focusing to generate microbubbles that contact the biofilm, and the pressure wave generated when the microbubbles are destroyed acts on the biofilm, so that at least a part of the biofilm is separated from the inner cavity wall. Further, when the liquid in the inner cavity and the extracellular material of the biofilm are focused by vibration, microbubbles will be generated on at least one of the surface of the biofilm, the interior of the biofilm, and between the wall of the inner cavity and the biofilm. Therefore, the pressure wave generated when the bubble is destroyed will act on at least one of the surface of the biofilm, the interior of the biofilm, and the space between the inner cavity wall and the biofilm, and then the biofilm or between the biofilm and the inner cavity wall will be generated. Clearance.

In a specific embodiment, the vibration assembly 25 is disposed on a phased array probe. Each vibration element is sequentially excited according to the distance between each vibration element and the target, so that each vibration element sequentially generates vibrations, and the energy of each vibration reaches the biofilm at the same time. Furthermore, the technology used in the phased array probe is to control each vibrating element to vibrate at different times, and these vibrations will be transmitted in the form of waves. Taking the position of the target object corresponding to the phased array probe as the center point, the vibration element of the phased array probe starts to vibrate in order from the vibrational element farther from this center point to the closer vibrational element in the same distance from the center point. The vibration elements will vibrate simultaneously. When the waves generated by each vibrating element reach the interference point at the same time, these waves will produce constructive interference, and then focus on the last interference point after constructive interference. In the present invention, the interference point is set on the biofilm, so that the biofilm is detached due to micro-bubbles generated by vibration. The above-mentioned way of focusing the vibration can avoid harming other unfocused tissues. When the energy is focused on the operating range around the biofilm, the vibration energy causes micro-bubbles to be generated in the cavity, and the micro-bubbles impact the biofilm 4 and disappear. Microbubble The generated pressure wave acts on the biofilm 4 so that at least a part of the biofilm 4 is separated from the inner cavity wall 50. In another specific embodiment, the emitted vibration energy is adjusted so that the vibration is focused into the cavity 5 to cause the liquid 51 to generate a vortex or turbulence, and at least a part of the biofilm 4 is detached from the vortex or turbulence by the vortex or turbulence.内腔 墙 50。 The inner cavity wall 50.

The frequency of the vibration generated by the vibration component 25 is between 0.3 MHz and 4.0 MHz. Specifically, the generated frequency may fall between 0.3 MHz, 0.5 MHz, 0.8 MHz, 1.0 MHz, 1.2 MHz, 2.0 MHz, 3.0 MHz, 4.0 MHz, or a value between the above frequencies.

In addition to guiding the magnetic beads close to the biofilm 4, the magnetron component 23 can further generate a high-frequency AC magnetic field to generate thermal energy to suppress the biofilm 4 and destroy the thermosensitive lipid film 30 to release the bacteriostatic substance 32. Both the high-frequency AC magnetic field and the high-frequency vibration have the effect of destroying the heat-sensitive fat film 30, and at the same time, they have a multiplier effect when they are applied to the heat-sensitive fat film 30, and quickly release more antibacterial substances 32 in the heat-sensitive fat film 30. .

Due to the principle of surface tension, the carrier 3 is substantially circular. The diameter of the carrier 3 is less than 0.22 μm, and it can be injected into the living body by injection or oral administration. With the precise removal method mentioned above, the total amount of medicament used at each time can be far less than the traditional way of removing biofilm with only medicament, and achieve the same removal effect. Precise, low-concentration drugs can prevent damage to other tissues or cells in the organism.

Please refer to FIG. 4 and FIG. 6. FIG. 6 is a schematic diagram of a carrier according to another embodiment of the present invention. The carrier 3 further includes a biofilm ligand 37. The first end of the biofilm ligand 37 is connected to the outside of the thermosensitive lipid film 30, and the second end of the biofilm ligand 37 is used to be connected to the biofilm 4. When the magnetic control component operation carrier 3 is close to the biofilm 4, the biofilm ligand 37 will be adsorbed on the biofilm. When the high frequency interactive magnetic field energy is emitted from the magnetic control component 23 of the cleaning device 2, the nano magnetic beads 38 vibrate and Generate heat to kill bacteria in the biofilm; when the cleaning device 2 emits energy to destroy the carrier 3, Bacterial substances can effectively contact the biofilm.

See Figure 5. FIG. 5 is a schematic diagram of a cleaning device according to another embodiment of the present invention. In a specific embodiment, the removal device 2 further includes a first probe 21. The first probe 21 includes the aforementioned magnetron component 23, vibration component 25 and a magnetic shield 24. The magnetic shield 24 is located between the magnetron component 23 and the vibration component 25 to reduce the electromagnetic effect of the magnetron component 23 on the vibration component 25. In this embodiment, the stability of the vibration component 25 can be maintained by the magnetic shielding 24. In addition, the magnetron assembly 23 and the vibration assembly 25 are concentrated on the first probe 21, which is advantageous for one-handed operation.

In another specific embodiment, the removal device further includes a third probe (not shown), the third probe is provided with a magnetron component, and the first probe is provided with a vibration component. In some cases, high-frequency vibration and magnetic fields must transmit energy from different angles of the cavity. At this time, the magnetron component and the vibration component need to be located at different probes, which is conducive to precision medical treatment.

The biofilm removal system 1 further includes a second probe (not shown), and the second probe has a detection component for receiving an echo, or can emit a vibration. The echo is the vibration generated by the vibration component 25, the vibration generated by the detection component, or A vibration generated by a third vibration source touches the cavity and the biofilm. Through the detection of the detection component, the precise position of the biofilm can be searched out, and then the biofilm is removed using the first probe 21. In a specific embodiment, the detection component may be located on the first probe.

In terms of application, the detection component, magnetron component, and vibration component can be set on three different probes, or any two can be on the same probe, or all three can be on the same probe, depending on the case or Practical needs.

In a category of the present invention, a biofilm removal system is further provided, which is used to remove a biofilm on an inner cavity wall of a cavity. The biofilm removal system includes a The cleaning device includes a detection component and a vibration component. The detection component is used to generate a first vibration, so that the cavity generates an echo corresponding to the first vibration, and the detection component further receives the echo. The vibration component further includes a plurality of vibration elements forming a phased array, and each vibration element sequentially generates a second vibration and the energy of the second vibration reaches the biofilm and a range thereof at the same time, so that the biofilm and the range thereof A thermal energy is generated inside, and the temperature of the biofilm is higher than the temperature of the inner cavity wall by the thermal energy. In a specific embodiment, the cavity system is the bladder, and the temperature of the inner cavity wall is the biological body temperature. The thermal energy generated in this way makes the temperature of the biofilm higher than the temperature of the bladder wall, and most bacteria will die at higher temperatures, thus achieving the effect of killing bacteria in the biofilm.

In another aspect of the present invention, a biofilm removal system is further provided for removing a biofilm on an inner cavity wall of a cavity. The biofilm removal system includes a carrier and a removal device. The carrier is located in the cavity, and includes a nanometer magnetic bead and an outer film for covering the nanometer magnetic bead. The cleaning device includes a vibration component and a magnetron component. The magnetron component is used to guide the carrier to or around the biofilm. The vibration component is used to generate a vibration to destroy the outer membrane and release the nano-magnetic beads. Next, the magnetic control component generates a high-frequency AC magnetic field to generate thermal energy by magnetically vibrating the nano-magnetic beads. The thermal energy causes the temperature of the biofilm to rise, thereby killing bacteria that are not resistant to high temperature inside the biofilm.

The biofilm removal system of the present invention provides various removal methods. Performing the following removal methods separately can achieve different effects; however, the following methods can also be combined with each other in a reasonable manner to achieve more versatile application methods.

A method for removing a biofilm in a cavity is applied to remove a biofilm attached to an inner cavity wall of a target. The method includes the following steps: providing a removal device including a first probe, the first probe including a vibration component and a magnetron component; and inputting a carrier into the target. The carrier contains bacteriostatic substances and nano-magnetic beads; the first probe is brought close to the corresponding outer surface of the target; the magnetic control component is controlled to bring the carrier close to the biofilm: the vibration component is controlled to generate a plurality of vibrations and the vibrations are focused to the organism On the membrane; using these vibration focusing to generate a plurality of microbubbles that contact the biofilm, so that at least a part of the biofilm is detached from the inner cavity wall, and the carrier releases the antibacterial substance.

Another method for removing the biofilm in the cavity is to inject the carrier into the cavity of the target; use the nanomagnetic beads of the magnetic control component to guide the nanomagnetic beads to drive the carrier to approach the biofilm; control the vibration component to generate a plurality of vibrations, and Vibration is focused to within a defined distance of the biofilm or one outside the biofilm; use these vibrations to focus to generate a number of microbubbles that contact the biofilm, so that at least a part of the biofilm is detached from the inner cavity wall; at the same time the energy of the vibration or vibration Thermal energy destroys the heat-sensitive lipid film to release the bacteriostatic substance. Because the carrier is close to the biofilm, the bacteriostatic substance can also smoothly affect the bacteria in the biofilm.

Another method to remove the biofilm in the cavity is to inject the carrier into the cavity; use the biofilm ligand on the carrier to specifically bind to the biofilm; control the magnetron component to generate a high-frequency AC magnetic field to make the nanometer magnet in the carrier Beads generate magnetic vibrations; magnetic vibrations generate thermal energy higher than the body temperature to kill bacteria in the biofilm; the thermally sensitive lipid film of the carrier is destroyed due to magnetic vibrations and the thermal energy generated by the magnetic vibrations, releasing bacteriostatic substances therein.

Another method for removing the biofilm in the cavity includes the following steps: close the vibration component to the corresponding outer surface of the cavity; control the vibration component to generate a plurality of vibrations and focus the vibrations on the biofilm; then, use these vibrations to focus and generate The plurality of micro-bubbles are contacted with the biofilm to disengage at least a part of the biofilm from the inner cavity wall.

In summary, the vibration component of the present invention is used to peel off most obvious biofilms, but it cannot remove all bacteria in the space. Bacteria can still cluster quickly after surgery, A biofilm is formed again. On the other hand, bacteriostatic substances cannot easily pass through the protection of the biofilm, and even if the dose is increased to the maximum, the bacteria in the biofilm cannot be completely removed. The system of the present invention further combines the advantages of the complementary two, peeling off most of the visible biofilm with the vibration component, and at the same time causing the carrier to release the bacteriostatic substance. Most of the peeled biofilm can be removed from the cavity by other means and removed , The remaining bacteria or free bacteria in the small amount of biofilm remaining in the cavity will be eliminated by the bacteriostatic substance. In addition to killing existing bacteria with bacteriostatic substances, it is also possible to carry environmental improvement substances in the carrier, making it difficult for bacteria to breed in the cavity. Therefore, the effect of the present invention is far superior to the existing biofilm cleaning technology. Compared with the conventional technology, the method for removing the biofilm in the cavity of the present invention can remove the biofilm deeper into the wall of the inner cavity than the previous surgical methods using physical debridement; the method of the present invention is more than today The removal method does not harm the patient's body tissue.

With the detailed description of the above specific embodiments, it is hoped that the features and spirit of the present invention can be described more clearly, rather than limiting the scope of the present invention with the specific embodiments disclosed above. On the contrary, the intention is to cover various changes and equivalent arrangements within the scope of the patents to be applied for in the present invention.

Claims (10)

A biofilm removal system is used to remove a biofilm on an inner cavity wall of a cavity. The cavity contains a liquid. The biofilm removal system includes: a carrier located in the cavity, which contains : A bacteriostatic substance for targeted inhibition of the biofilm; and a nanometer magnetic bead; and a removal device including: a magnetron component for guiding and magnetically vibrating the nanometer magnetic bead; and The vibration component is used for generating a vibration to generate a plurality of micro-bubbles in the liquid, so that at least a part of the biofilm is separated from the inner cavity wall, and the carrier is destroyed to release the bacteriostatic substance. The biofilm removal system described in item 1 of the scope of the patent application, wherein the vibration component further includes a plurality of vibration elements, and each vibration element sequentially generates the vibration and the energy of the vibration reaches the biofilm at the same time. The biofilm removal system according to item 1 of the patent application scope, wherein the removal device further includes a first probe and a magnetic shield, the magnetic shield, the magnetron component and the vibration component are disposed on the first probe, and The magnetic shielding is located between the magnetron component and the vibration component to reduce the electromagnetic effect of the magnetron component on the vibration component. The biofilm removal system according to item 1 of the patent application scope, wherein the magnetron component is further used to generate a high-frequency AC magnetic field, and the carrier further includes a heat-sensitive lipid film to cover the bacteriostatic substance and the Nanometer magnetic beads, and when the carrier receives the vibration generated by the vibration component or the high-frequency AC magnetic field generated by the magnetron component, the heat-sensitive lipid film is destroyed to release the bacteriostatic substance. The biofilm removal system according to item 4 of the scope of the patent application, wherein the carrier further comprises a biofilm ligand, a first end of one of the biofilm ligands is joined to the outside of the thermosensitive lipid film, and the biofilm ligand A second end of one of the bodies is used to join the biofilm. The biofilm removal system according to item 1 of the scope of patent application, wherein the bacteriostatic substance is selected from the group consisting of antibiotics, radioactive substances, antibodies, exothermic compounds, vaccines, alcohol, disinfectants and bactericides At least one of them. The biofilm removal system according to item 1 of the scope of the patent application, wherein the frequency of the vibration generated by the vibration component is between 0.3 MHz and 4.0 MHz. A biofilm removal system is used to remove a biofilm on an inner cavity wall of a cavity. The cavity contains a liquid. The biofilm removal system includes: a carrier located in the cavity, which contains : An environment-improving substance for improving the environment in the cavity, the environment-improving substance is at least one selected from the group consisting of beneficial bacteria, acidic compounds, basic compounds, nutritional agents, and bioremediation factors And a nanometer magnetic bead; and a cleaning device including: a magnetron component for guiding and magnetically vibrating the nanometer magnetic bead; and a vibration component for generating a vibration to generate a plurality of numbers in the liquid Micro-bubbles, disengage at least a part of the biofilm from the inner cavity wall, and destroy the carrier to release the environment-improving substance. A biofilm removal system for removing a biofilm on an inner cavity wall of a cavity. The biofilm removal system includes: a carrier located in the cavity, which includes: a nanometer magnetic bead; and An outer film to cover the nano magnetic beads; and a cleaning device including: a vibration component for generating a vibration to destroy the outer film to release the nano magnetic beads; and a magnetic control component, It is used to guide the carrier to the biofilm and generate a high-frequency AC magnetic field to magnetically vibrate the nano-magnetic beads to generate a thermal energy to raise the temperature of the biofilm. According to the biofilm removal system described in item 9 of the scope of patent application, wherein the cavity contains a liquid, the vibration component further focuses the vibrations into the cavity to cause the liquid to generate a vortex or a turbulent flow. At least a part of the biofilm is separated from the inner cavity wall by the vortex or the turbulence.