TW509722B - Insecticidal protein toxins from photorhabdus - Google Patents

Abstract

Proteins from the genus photorhabdus are toxic to insects upon exposure. Photorhabdus luminescens (formerly Xenorhabdus luminescens) have been found in mammalian clinical samples and as a bacterial symbiont of entomopathogenic nematodes of genus Heterorhabditis. These protein toxins can be applied to, or genetically engineered into, insect larvae food and plants for insect control.

Description

Printed by the Consumer Cooperatives of the Central Bureau of Standards of the Ministry of Economic Affairs. 5. Description of the invention (1; Technical Phoenix 1 please file the case of the old lady t. Special: fl towel M case—US patent application filed on November 6, 1 Partial Continuation Application Serial No. 08 / 743,699, which is a partial continuation application of US Patent Application Series No. 08/705, 484 filed on August 28, 1996. The previous case was-1996 2 Partial continuation of US Patent Application Serial No. 08/608, 423 filed on March 28. The previous case was a US Patent Application Serial No. 08/395, 947 filed on February 28, 1995 Part of the continuation application. The previous case was a part of the continuation application of US Patent Application Serial No. 08 / 〇63, 615 filed on May 18, 1993. This application is also a Part of the US Patent Provisional Application Series No. 60/007, 25 filed on June 6th, the continuation of the application. The invention is related to toxins isolated from bacteria and the use of these toxins as pesticides. Background of the Invention Many insects are occupants, picnicers, gardeners, farmers, and other investors Those who are susceptible to pests are regarded as pests. Especially in short growing seasons, 'severe pests cause total loss of farmers' income and a large reduction in farmland. The lack of supply of specific agricultural products will inevitably lead to higher costs for food processors, which in turn will Prices of food plants and products derived from these plants have risen. Protecting crops and flowers from pests and clearing them has traditionally relied on powerful organic pesticides with widespread toxicity. These synthetic products are due to their environmental and exposure to these agents It ’s harmful and attacked by the public. Also, please pay attention to MI first, then 1 side binding • 4- This paper size applies Chinese national standard (〇 奶) 8 4 ^ (210 > < 297 mm) 509722 A7 B7 5 2. Description of the invention (2) In non-agricultural areas, residents are satisfied with having insects to protect their home or outdoor food without killing them. The widespread use of chemical pesticides has caused farmers, companies producing pesticides, Environmental and health concerns of government agencies, public interest groups, and the general public. The development of low-interference pest control strategies Society's attention to the environment and the development of biological tools using insect control mechanisms have been encouraged. Biological control agents have shown alternatives to chemical pesticides. At every stage of evolution, organisms have conceived ways to improve their Success and survival. The application of biomolecules as defense and attack tools is widely known in the animal and plant world. In addition, newer tools of genetic engineering allow the modification of biological pesticides to solve specific problems. A kind of bismuth Good: h% QBacillus thuringiensis (Bt) is an effective insecticide and is widely used commercially. In fact, the insecticide of this valence bacterium is a very toxic protein, which can be used as a human food crop at the moment of harvest. For non-standard organisms, the valence toxin is a digestible non-toxic protein. Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs. Another known type of biological insect control agents is certain nematodes, which are known as a vector for insecticidal bacterial symbiotics. Contains insecticidal bacterial nematodes that invade insect larvae. The bacteria then killed the larva. The nematode reproduces inside the larvae's body, and the larvae then devour the carcass from the inside. Nematode larvae containing this bacterium are formed and can invade other larvae. The insect-killing nematodes that met SteinernemaMi and 晷 release green, epithelium {heterorhabditis, were used as insect control agents. Obviously, each nematode is -5- This paper size applies to the Chinese National Standard (CNS) A4 specification (210X297 mm) " 509722 A7 B7 V. Description of the invention (3) Host of a specific bacterial species. In the worms of the genus Nematode, the symbiotic bacteria are first-released and first-released iPhotorhabdus (Iwainescens). Although their nematodes are effective insect control agents, their production, preservation, and distribution as insect control are still difficult, expensive, and inefficient. In terms of skill, we know that an insecticidal toxin can be isolated from G. luminifera, which is active only when injected into lepidopteran and coleopteran insect larvae. This makes it impossible to effectively utilize the insecticidal properties of the nematode or its bacterial symbiote. Applicable persons should use a more practical and less labor-intensive method for spreading insecticidal toxins, which can retain their biological properties after spreading. I am anxious to discover an orally active toxin produced by Photobacterium. For reasons of efficiency, the separation and application of such toxins is as desired. Until the applicant of this case found out, their toxins had not been isolated or given their properties. SUMMARY OF THE INVENTION These natural toxins are protein complexes produced and secreted by bacterial cells growing in the genus Corynebacterium. They are proteins produced by those interested by Corynebacterium radiata. These protein complexes having a molecular weight of about 1,000 kDa can be separated into multiple constituent proteins by SDS-PAGE gel analysis. These toxins do not contain hemolysin, lipase, C-type phospholipase or nuclease activity. These toxins can be significantly toxic to many insects. The present invention provides an insecticidal protein that is easy to administer and the expression of endotoxins in heterologous systems. The present invention also provides a method for dispersing insecticidal toxins, and the paper size of these poisons applies the Chinese National Standard (CNS) A4 specification (210X297 mm). Read the precautions of η f δτ before raising the Central Bureau of Standards of the Ministry of Economic Affairs. Printed by Employee Consumer Cooperatives 509722 A7 B7 V. Description of Invention (4) The element can maintain functional activity and is effective for many orders of insects. The objects, advantages and features of the present invention will be shown in the following patent specifications. Brief Description of the Drawings Figure 1 shows the pairing of selected DNA isolates which are partial sequence genes of the toxin of the present invention. Figure 2 is a map of the three plastids used in the sequencing method. Figure 3 is a map showing the interrelationship of a plurality of DM partial fragments. Figure 4 shows homogeneity analysis between protein protein sequences. Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (also read the notes on f% before filling out this page) Figure 5 shows the evolutionary tree of Bacillus strains. The relationship between Bacillus strains was determined by rep-PCR. The upper axis of FIG. 5 is the percentage of similarity measured on the basis of the score of the rep-PCR product (ie, 〇 [no similarity] to 1.0 [100% similarity]). On the right axis, these numbers and letters represent the different strains tested; 14 = W-14, Hm = Hm, Η9 = Η9, 7 = WX-7, 1 = WX-1, 2 = ffX-2, 88 = HP88, NC-1 = NC-1, 4 = WX-4, 9 = WX-9, 8 = WX-8, 10 = WX-10, WlfHHR, 3 = WX-3, 11 = WX-11, 5 = WX-5, 6 = ffX-6, 12 = WX-12, 14 = ffX-14, 15 = ffX-15, Hb = Hb, B2 = B2, 48 to 52 = ATCC 43948 to ATCC 43952. The vertical line of the divergent horizontal line represents the degree of correlation between the strains or strain groups at the base of the horizontal line (extending from the vertical line to the intersection with the upper axis; for example, the W-14 strain is about 60% similar to the H9 and Hm strains). Figure 6 shows the genome map of W-14 strain. Figure 6A shows the tea and tcb segments and the primary gene product. Figure 7 shows the evolutionary tree of Corynebacterium strains determined by rep-PCR. Figure 7 This paper size applies the Chinese National Standard (CNS) A4 specification (210X297 mm) 509722 A7 B7 V. Description of the invention (5) The upper axis is the percentage of similarity measured based on the score of the rep-PCR product (Ie, 〇 [no similarity] to L0 [100% similarity]) On the right axis, these numbers and letters represent different strains tested. The vertical line of the divergent horizontal line represents the degree of correlation between the strains or strain groups at the base of the horizontal line (extending from the vertical line to the intersection with the upper axis; for example, the Indicus strain is about 30% similar to the MP1 and HB Oswego strains). The bacillus strains on this evolutionary tree are as follows: 14 = W-14; Hm = Hm; H9 = H9; 7 = WX-7; 1 = WX-1; 2 = WX-2; 88 = HP88; NC1 = NC -1; 4 = WX-4; 9 = WX-9; 8 = WX-8; 10 = WX-10; 30 = ff30; WIR = ffIR; 3 = ffX-3; ll = ffX-ll; 5 = ffX -5; 6 = WX-6; 12 = WX-12; 15 = WX ~ 15; Hb = Hb; B2 = B2; 48 = ATCC 43948; 49 = ATCC 43949; 50 = ATCC 43950; 51 = ATCC 43951; 52 = ATCC 43952 〇 Detailed description of the invention Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs The present invention finds a unique type of insecticidal protein toxin derived from the genus Corynebacterium, which has oral toxicity against insects. One of the unique properties of photobacteria is its bioluminescence. Bacillus can be isolated from different sources. One source is nematodes, and the more specific is the nematodes of the genus Heterophylla. Another source is from human clinical trauma specimens (see Farmer ei a / · 1989 J · Clin · Microbiol · 27pp. 1594-1600) 〇 Saprophytic strains are deposited in the American Type Culture Collection, Rockville , MD) ATCC #s 43948, 43949, 43950, 43951 and 43952, and are incorporated herein by reference. Other sources may also contain bacteria of the genus Corynebacterium that produce insecticidal toxins. These sources in the environment can be land or water. -8- This paper size applies the Chinese National Standard (CNS) A4 specification (210X297 mm) '' 509722 A7 __B7_ V. Description of the invention (6) The genus Bacillus genus is classified as a member of the family of Enterobacteriaceae in taxonomy Although it has some traits that are not typical. For example, the strain of this genus is negative for nitrate reduction, produces yellow and red pigments, and is bioluminescent. The latter trait is unknown in the Enterobacteriaceae. Bacillus bacillus has only recently been described as being isolated from a genus of Je / iorAaMi / s (Boemare a / ·, 1993 Int · J · Syst · BacterioL 43, 249-255). This difference is based on DNA-DNA hybridization studies, phenotypic differences between catalytic enzymes and bioluminescence with or without (bacillus bacilli), and the family of nematode hosts (heterobacteria; family, bacilli Fungi; Heteromorphidae). Comparative cellular fatty acid analysis (Janes ei 5 / · 1990, Lett · Appl · Microbiol. 10, 131-135; Suzuki et al. 1990Gen. Appl. Microbiol., 36, 393-401) supports Corynebacterium A. autologous Bacillus was isolated. To confirm the disclosure, the strain collections here consist of Bacillus spp. Strains, which are based on defining the traits of Bacillus spp. And distinguishing traits from other enterobacteriaceae and heterobacteria species. (Farmer, 1984 Bergey's Manual of Systemic Bacteriology Vol. 1 pp. 510-511; Akhurst and Boemare 1988 J. Gen. Microbiol. 134 pp. 1835-1845; Boemare et al. 1993 Int. J. Syst. Bacteriol. 43: 249-255, which is incorporated herein by reference) These traits have been studied as follows: Gram-negative bacilli, cell size, colony pigmentation, inclusion bodies, presence of catalytic enzymes, nitrate reducing capacity, Bioluminescence, dye absorption, gelatin hydrolyzability, growth in selective medium, growth temperature, survivability under anaerobic conditions, motility. This paper size applies to China National Standard (CNS) A4 (210 X 297 mm). Please read the note above.

I Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 509722 A7 B7 V. Description of the invention (7) Fatty acid analysis was used to confirm that the strains belonged to a single Bacillus genus. At present, the genus Corynebacterium is composed of a single defined species, and the bacterium Corynebacterium radiata (ATCC-type strain # 29999, Poinar ei a / ·, 1977, Nematologica 23, 97-102). Different related strains have been described in the literature (for example, Akhursteia 1988 J. Gen. Microbiol., 134, 1835-1845; Boemare et al. 1993 Int. J. Syst. Bacteriol. 43 pp. 249-255; Putz et. al. 1990, Appl. Environ. Microbiol., 56, 181-186). Multiple Bacillus strains have been characterized herein. As there is currently only one bacterial species (Galleria radiata) that is defined within the genus Corynebacterium, the traits of this species are used to characterize these strains herein. As shown in Figure 5, these strains are quite divergent. In the foreseeable future, there will be other species of Bacillus bacillus, which will have the properties of Bacillus radiata, and also have some characteristics that have not yet been defined as the traits of Bacillus radiata. However, the scope of the present invention is to be able to produce any Bacillus species that have a functional activity as a protein for insect control, and do not consider other traits and characteristics. Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs. Furthermore, as shown in this article, bacteria of the genus Bacillus can produce proteins with functional activities defined below. Of particular interest are proteins produced by light-emitting Corynebacterium. The invention herein is not limited to the strains disclosed herein. These strains are the first to show that proteins produced by different isolates of Corynebacterium are toxic to exposed insects. Therefore, the following inventions include the strains specified in this document and any variants thereof, as well as any of the following functional activities of the genus Corynebacterium -10- This paper applies the Chinese National Standard (CNS) A4 specification ( 210X 297 mm) 509722 A7 B7 V. Strain or strain of invention description (8). Many terms used herein have the following special meanings: "functional activity" means here that the protein toxin has the function of an insect control agent. The protein is orally active, or has a toxic effect, or can interrupt or interfere with eating, which does not necessarily cause insect death. When an insect comes into contact with an effective amount of a toxin spread through the expression of a transgenic plant, a prescription plant composition, a sprayable protein composition, a bait matrix, or other distribution system, the result is usually the death of the insect, or the insect cannot Food sources of toxins. The term "genetic material" is used herein to include all genes, nucleic acids, DNA and RNA. "Homogeneous" refers to a monoamino acid sequence that has been identified as having homology with a reference W-14 toxin polypeptide amino acid sequence. Printed by the Consumer Standards Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (please read the notes on the back before filling this page) "Homogeneity" refers to a monoamino acid sequence, which has a similarity index of at least 33% and / or has a reference The W-14 toxin polypeptide amino acid sequence has a similarity index of at least 26%, which uses the BlOsum 62 protein score matrix (Wisconsin Package Version 9.0, Genetic Computer Group (GCG), Madison, WI) to score by GAP calculations . "Identity" refers to a monoamino acid sequence, which has the same residues at a specific position by GAP calculation, which conforms to the arrangement of a reference W-14 toxin polypeptide amino acid sequence. The protein toxins discussed herein are generally used as insecticides. Insecticide means herein that the protein toxins have functional activity as defined later and are used as insect control agents. -11- The dimensions of this paper apply to Chinese National Standard (CNS) A4 (210X297 mm) 509722 A7 ______ B7 V. Description of the invention (9) The use of the term "oligonucleotide" refers to the short-term use of RNA or DNA. A giant molecule composed of chain nucleotides. This length is at least 1 nucleotide, but is usually in the range of 10 to 12 nucleotides. Oligonucleotide length is determined by those skilled in the art and is not limited in this article. Therefore, the oligonucleotide may be less than 10 or greater than 12. The use of the term bacillus toxin refers to any protein produced by a bacillus microorganism strain that has a functional activity against insects. The bacillus toxin is formulated there to be a sprayable composition Species, expressed by transgenic plants, formulated as a bait matrix, spread by baculovirus, or spread by other applicable hosts or distribution systems. The term "toxic" or "toxic" The use herein means that the toxins produced by Corynebacterium have functional activity as defined herein, and "truncated peptides" herein include any peptides that were originally peptides. The fragment has functional activity after being observed. The "actual sequence homogeneity" printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs can have one of the following meanings. A DNA fragment with one nucleotide sequence is sufficient to DNA fragments are similar and can produce proteins with similar biochemical properties; or a polypeptide has a biochemical sequence sufficient to show similar biochemical properties to another polypeptide. The fermentation broths of the selected strains shown in Table 20 were used to determine the following: the breadth of the insecticidal toxins produced by the genus Corynebacterium, the insecticidal spectrum of these toxins, and the raw materials that provide purification of these complexes. These strains are characterized in this article to show that they have oral toxicity against different insect orders. These insect orders include Coleoptera, Homoptera, Scales _____- 12- This paper applies Chinese National Standard (CNS) A4 Specifications (210X297 public shame) 509722 Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs A7 __B7___ V. Description of the invention (10) Hymenoptera, Diptera, Taenia, Hymenoptera and Netoptera, but not limited to this As for other bacterial toxins, the mutation rate of the bacteria in the population leads to the existence of many related toxins that differ slightly in sequence. The toxins of interest here are those that can produce protein complexes that are toxic to different exposed insects, as in As described herein, the toxins are active against Lepidoptera, Coleoptera, Homoptera, Diptera, Hymenoptera, Reticoptera, and Taenia. This invention intends to obtain Protein toxins that are homogeneous to protein toxins produced by these strains and any derivative strains herein, and any protein toxins produced by Bacillus difficile. The sequence of this equivalent protein may be different, but There is no difference in the function of the toxins described in this article. Homogeneous toxins include protein complexes between 300kDa and 2,000kDa, which are composed of at least two subunits, one of which is one phthalate, It may not be the same as other subunits. Different protein subunits have been identified and taught in the examples herein. Usually these protein subunits; between about 18 KDa to about 230 kDa; between about 160 KDa to about Between 230 kDa; between 100 KDa and 160 kDa; between approximately 80 KDa and approximately 100 kDa; and between approximately 50 KDa and approximately 80 kDa. As discussed above, certain Bacillus strains can be isolated from nematodes. Certain nematodes (the long cylindrical parasites of the nematode phylum) have evolved the ability to use insect larvae as a suitable growth environment. This insect larva provides a growing nematode food source and its reproduction environment. One of the main effects of larvae invaded by certain nematodes is the death of larvae. The death of larvae is attributed to certain lines-13- This paper size applies Chinese National Standard (CNS) A4 size (X297 mm) (Please read the precautions on the back before filling this page) 4 509722 A7 B7 V. Description of the invention (11) There are bacteria in the worm body, which can produce an insecticidal toxin to hinder the growth of larvae and inhibit their feeding activities. Interestingly, it seems that each genus of insect parasitic nematodes resides in a special species of bacteria that are uniquely adapted to grow symbiotically with the nematode. Period Since the beginning of this study, the names of the genus Bacillus were reclassified as heterobacteria and Bacillus. The bacteria of the genus Bacillus genus are endowed with the characteristics of the symbiosis of the worm Nematode, which is a symbiote of the species. This change in nomenclature is reflected in this patent specification, but the change in nomenclature does not affect the scope of the invention described below. The peptides and gene lines disclosed herein are named according to the standards recently published in the “Author's Guide” to the Journal of Bacteriology, pages i-xii (January 1996), which are incorporated herein by reference. The following peptides were isolated from the gene line from the strain Corynebacterium W-14. Table 1 Peptide / gene nomenclature, toxin complex (I read the precautions on the back before filling this page) Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs 1 Peptide name 2 Peptide identification sequence number * 3 4 Gene gene name Identification sequence number * ica gene body region TcaA 34c tcaA 33 TcaAi quasi-peptide tcaA-TcaAu [15] a, 34 c tcaA-TcaAiii [4] a, 35c tcaA-TcaAiv [62] a tcaA — TcaB [3] a, (19,20) b, 26c tcaB 25 TcaBi [3] a, (19,20) b, 28c tcaB 27 TcaBu [5] a, 30 c tcaB 29 TcaC [2] a, 32 c tcaC 31 genomic region TcbA 12 c, [16] a, (21, 22 tcbA 11 23,24) b TcbAi quasi-peptide tcbA 1-14- This paper size applies to China National Standard (CNS) A4 specification (210X297 mm) 509722 V. Daming Explanation (l2) _ TcbAu [l] a, (21, 22, 23, 24) b, 53 c TcbAni [40] a, iec gene body region ”P1 domain [8 [76 region body factor ABC ^ cc C cc CC TTT t 55 57 c 59 c

TcdA (17, 18, 37, 38, 39, 42, 43) b, 47 c TcdAi quasi-peptide TcdAn [13] a, (17, 18, 37, 38, 39) b, 49 c TcdAiU [41] a , (42,43) b 51 c TcdB [14] a A7 B7 tcbA 11 tcbA 54 tccA 56 tccB 58 tccC 60 tcdA (36) d, 46 tcdA-tcdA 48 tcdA 50 tcdB — (I read first ^: note of the above Please fill in this page again) a) The sequence identification number in square brackets is the peptide N-terminus. These sequences printed by the Central Bureau of Standards Consumer Cooperatives are grouped by genome regions. More precisely, the light-emitting Corynebacterium (W-14) has at least 4 different genomic regions ica, icZ ?, icc, and icd. As seen in Table 1, the peptide product was generated from these different genomic regions. Furthermore, as shown in the examples (especially Examples 15 and 21), individual gene products generated from the three genomic regions are related to insect activity. There is also considerable homogeneity among these four genomic regions.

As shown in the examples, this gene appears as two possible biologically active protein fragments (TcbA and TcbA i i / i i i) in E. coli. The ic heart gene is also expressed in E. coli. As shown in Example 16, when the untreated TcbA toxin is endogenous metalloproteinase or protease-containing _-15- This paper size applies the Chinese National Standard (CNS) A4 specification (210X297 mm) 509722 A7 B7 V. Description of the invention (l3) In the case of insect intestinal contents, the TcbA toxin is processed into smaller subunits, which are smaller in size than natural peptides and increase the activity of southern corn rootworms. These smaller toxins are still attached as part of the toxin complex. In some cases, please read the note of l. TA fill in% of this page. In some cases, you can expect to increase the activity of these toxins by proteolytic treatment or by using truncated peptides. Therefore, it is expected that truncated peptides will be used in certain applications, such as commercial genetically modified plant applications. In addition to this W-14 strain, other species of the genus Corynebacterium have different functional activities (see especially Examples 20 and 36). Even if the activities are different, some of these amino acid sequences have comparable sequence homogeneity. Furthermore, the molecular probe showed that certain genes contained in the strain were homogeneous with the genes contained in the w-14 strain. Virtually all of the strains shown in this article have homology to one or more W-14 toxin genes. The antibody data in Example 26 and the N-terminal sequence in Example 25 further support the conclusion that the protein toxins produced by these strains are homogeneous and identical (based on the amino acid sequence). At the molecular level, the W_14 gene probe shows that the homogenous substance or the W-14 gene itself is widely present in the genus Corynebacterium. Furthermore, the 'neotoxin gene may exist in other strains printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs, which are not homogeneous with W-14, but still retain all the protein properties (see especially Examples 14 and 25). Even if the toxin genes produced by Corynebacterium are homogeneous and identical, these strains themselves are quite different. Using the polymerase chain reaction discussed in Example 22, most of the strains shown herein are fairly easy to identify. For example, as shown in Figure 5, the relative similarity of the percentages of some strains (such as swollen ribs and muscles) is about 0.8, which shows that these strains are similar; however, the liver 88 and costal paper scales are applicable to Chinese national standards (CNS) A4 specifications (210X297 mm) • 16- Printed by the Consumer Standards Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs A7 B7 V. Description of the invention (14) I '~ ~ 0.1, showing that these strains are different. Therefore, even if the insect toxin genes or the strain's gene products are identical or similar, the strains themselves are still quite different. Rong Yu ^ ^ ^ Signed in the information disclosed in these examples and the discussion in this article 'Obviously 4 new and unique insecticidal protein toxins have been released. These' primes are widely present in the genus Bacillus This strain has been shown within the text *. This is an example where these toxins are widespread in the enterobacteriaceae. The anti-Zhao prepared as described in Example 21 or the genetic probe prepared as described in Example 25 can be used for further screening of enterobacteriaceae to produce functionally active homogeneous toxin strains. It can also be an example where the presence of a new gene can be identified in the genus Corynebacterium or Enterobacteriaceae. As described above, these antibodies can be used to quickly screen homogeneous toxin products as shown in Example 26 in the genus Corynebacterium or enterobacteriaceae. The technique in this technique is quite similar to using antibodies as an analysis or screening tool (see U.S. Patent No. 5,430,137, incorporated by reference below). Furthermore, antibodies are induced to counteract 6 to 20 amino acid residue segments that tend to occupy the exposed surface of dopeptide, which are generally accepted in the literature (Current Protocols in Immunology, Coligan et al., National Institute of Health, John Wiley & Sons, Inc.). The amino acid is usually composed of contiguous amino acid residues, however, in some cases it may be constituted by non-contiguous amino acids, which are limited by special configurations. This amino acid segment recognized by the antibody is highly specific and is often referred to as an epitope. The amino acid fragment can be generated by chemical and / or enzyme cleavage of natural proteins, automatic solid-phase peptide synthesis, or production from genetically engineered organisms. Duosheng version clips can be based on the differences in the art and / or liver ^ and FPLC color layer -π-_ This paper size applies the Chinese National Standard (CNS) A4 specification (21〇 > < 297mm) ( (Please read the notes on the back before filling out this page) 1. -Order · 509722 A7 B7 printed by Central Standards X Consumer Cooperatives V. Description of Invention (15) Combination of analysis methods. The selection of peptide fragments can be exploited by calculations (eg, Kyte and Doolittle, 1982, Journal of Molecular Biology 157: 105-132 and Chou and Fasman, 1974,

Biochemistry 13: 222-245, which predicts which sequences are most likely to be exposed to the surface of a protein). In order to prepare the immunogen containing the polypeptide fragment of interest, the polypeptide usually uses a chemical reaction to borrow an independent amino acid (lysine), a hydrogen sulfide (cysteine), and a phenyl (tyramine). Acid) or carboxyl groups (aspartic acid and glutamic acid) are covalently paired with carrier proteins such as keyhole limpet hemocyanin. An immunogen containing an adjuvant is injected into an animal such as a mouse, rat or chicken to induce an immune response against the immunogen. The analysis of antibodies against polypeptide fragments in the serum of injected animals can be determined by different immunological methods such as Elisa and Western transfusion. Alternatively, a single-source antibody can be prepared by injecting an animal's spleen cells and tumour cells to produce immortal fusion tumor cells (which can produce a single antibody species). The fused tumor cells were selected by immunological methods to produce cell lines that produced antibodies against the Tokachi fragments of interest to us. Purification of antibodies from different sources can be performed by HPLC or FPLC methods with different antigen affinity or antibody affinity columns and other chromatography. The toxins described herein are quite unique and have critical functional activities in developing a pest control strategy. In developing an insect control strategy, it may be possible to delay or overcome protein degradation by directly injecting the animal and avoiding the digestive tract. In these examples, the protein applied to the animal will retain its function until it is degraded and non-specifically degraded. • 18- This paper is suitable for financial standards _ home standard ^ grid (2ιχχ297)---- ------ • Equipment— (Please read ^: Precautions before filling in this page) Order 509722 A7 V. Description of the invention (16 Or use the immune system to expel it in other organisms such as Southern Titanium. One insecticidal The injection of toxins into insects is practical only in the laboratory, and only in large insects that are easy to inject. The insecticidal protein toxins described in this article show toxicity after oral ingestion or contact with the toxins, making them able to An insect control program was developed based solely on the ability to incorporate the protein toxin into insect baits. This program can lead to the production of insect baits. The bacillus toxins can be applied to insects in purified form. The toxins can also be used per liter The broth is distributed in an amount of about 1 to 10 Offlg. This varies depending on the formulation conditions, the conditions of the source of inoculation, the technique of toxin separation, etc. These toxins can exudate secretions or originate from heterologous prokaryotic or eukaryotic host manifestations Cellular proteins are applied. Bacteria are usually hosts for protein expression. Eukaryotic hosts can include, but are not limited to, plants, insects, and yeasts. Optionally, the toxins can be produced from bacteria or genetically transfected in the field Plants' or baculovirus vectors are produced from the body. Usually the toxins enter the insect body by incorporating one or more toxins into the food of the insects. It is effective to kill all the insects that eat it, But it is not necessary to achieve effective toxicity. If the nettle evades the toxin or stops feeding, even if the specific effect is subiethal, it is also useful for some applications. For example, hypothetical insect resistance Sexually transgenic crops are what they want, and insect rejection of these plants is as useful as their lethal toxicity to these quinces, because the ultimate purpose is to focus on the protection of these plants rather than killing them. There are many other ways to incorporate toxins into an insect's secluded food. Such as 1 ... I I. (Please read the notes on the back before filling out this page),? Τ Staff of the Central Standards Bureau of the Ministry of Economic Affairs Printed cooperatives fee

509722 A7 _______B7 V. Description of the invention (Π) In the example, it is possible to spoil the larval food source by spraying Li-protein solution on the food. Alternatively, the purified protein can be genetically engineered into an otherwise harmless bacterium, which can then be grown by culture, applied to the food source, or allowed to remain in the soil of an insect-fighting area as desired. The protein can also be genetically engineered to directly access an insect food source. For example, the main food source for many insect larvae is plants. The Consumer Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs printed the genetic material that encodes the insecticidal nature of the bacillus toxin into the gene of a plant ingested by a particular pest. death. Many monocotyledonous and dicotyledonous plants have been transformed. Genetically modified agronomic crops and fruits and vegetables are of commercial interest. These crops include, but are not limited to, corn, rice, soybeans, Xiang Yue sunflower, alfalfa, millet, wheat, cotton, peanuts, potato and so on. Many techniques exist to introduce foreign genetic material into plant cells and to obtain plants that stably maintain and express the introduced genes. These techniques include the promotion of genetic material encapsulated in microparticles that enter the cell directly (U.S. Patent No. 4,945,050 issued to Cornell University and 5,141,131 issued to DowElanco). Plants can be transformed using the T. velutii) technology, see US Patent No. 5,177,010 issued to Toledo University, No. 5,104,310 issued to Texas A & M University, and European Patent Application No. 013162461 European Patent Application Nos. 120516, 159418B1, 176, 112 issued to Schilperoot, U.S. Patent Nos. 5,149,645, 5,469,976, 5,464,763 issued to Schilperoot No., -20- This paper size applies the Chinese National Standard (CNS) A4 specification (21 × 297 mm) 509722 A7 B7 ___ V. Invention Description (is) No. 4,940,838, No. 4,693,976, issued to Xianyi? 1 & 1 ^ 1 ^ European Patent Application Nos. 116718, 290799, 320320, and European Patent Application Nos. 604662 and 627752 issued to Japan Tobacco 7, European Patent Application Nos. 0267159, 0292435 and US Patent No. 5,231,059, US Patent Nos. 5,463,174 and 4,762,785 issued to Calgene, and "3〇61: 113" US Patent Nos. 5,004,863 and 5,159,135. Other transformation technologies include Wiskers technology, see U.S. Patent Nos. 5,302,523 and 5,464,765 issued to Zeneca. Electroporation technology has also been used to transform plants, see WO 87/06614 issued to Byyce Thomps Institute and 5,472,869 and 5,384 issued to Dekalb No. 253. WO9209696 and W09321335 issued to PGS. All such transformed plants are incorporated into the publication with reference. In addition to a variety of techniques for transgenic plants, the types of tissues that come into contact with foreign genes can also vary. This tissue may include (but is not limited to) embryogenic tissue, type I and type II healing tissue, hypocotyls, meristems, and the like. Almost all plant tissues can be transformed during differentiation using appropriate techniques familiar to those skilled in the art. Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs Another changeable option is the selection mark. The preference of a particular marker is the judgment of those skilled in the art, but any of the following selection markers can be used with any other gene not listed here, which can function as a selection marker. These selectable markers include (but are not limited to) the amine ligand phosphotransferase gene of the Tn5 transposon, which encodes the resistance to the antibiotic kanamycin; neomycin and G418; 2 The size of the paper is in accordance with the Chinese National Standard (CNS) A4 (210X297 mm). It is printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs. ; Hygromycin; methotrexate; phosphinothricin; imidazolinones; continued fermentation with urea and triazolopyrimidine herbicides, such as gas Saffron (chlorosulfuron); bromooxynil and dalapon. In addition to selecting genes, the use of reporter genes is also desirable. In some cases, a reporter gene can be used without a selectable marker. Reporter genes are genes that are not normally present or expressed in the organism or tissue in which they are received. The reporter gene usually encodes a protein that provides properties as a change in certain phenotypes or enzymes. Examples of such genes are found in K. Weisingeia / · Ann · Rev. genetics, 22,421 (1988), which is incorporated in the reference. A preferred reporter gene is the uroglycerase (GUS) gene. Regardless of the transformation technique, the gene is incorporated into a gene delivery vector, and it is preferred that it can express bacillus toxin in plant cells by containing a plant promoter. In addition to plant promoters, promoters from different sources can be effectively used to express foreign genes in plant cells. For example, bacterial origins such as the octopine synthase initiator, the nopaline synthase initiator, the mannopine synthase initiator; the viral origin initiator, Such as cauliflower mosaic virus (35S and 19S), reconstructed 35S (referred to as 35T, see PCT / US96 / 16582, WO 97/13402, which was published on April Ⅱ, 1997, and incorporated herein by reference) Similar ones can also be used. Plant starters include (but are not limited to this) 1,6_ ribulose carboxylase (RUBP) minor _ -22- This paper size applies to China National Standard (CNS) A4 (210X297 mm) ( (Please read the notes on the back before filling out this page)

, 1T State 722 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs A7 B7 V. Description of the Invention (20) ~ ---- Units (_, Zhenta Total Soybean Plaque Promoter, Phaseolin Promoter, ADH From Promoters, thermal shock initiators, and tissue-specific promoters. Promoters may also contain some promoter sequence components that promote transcription efficiency. Code = promoters include (but are not limited to) Adh- Interval 1 and Adh 6. Intergranular promoters are available. Intrinsic promoters continually direct continuous gene expression in all cells (such as actin, ubiquitin) , CaMV 35S). Tissue-specific initiators are responsible for specific cell or tissue types, such as gene expression of leaves or seeds (such as maize protein, oleosin, napin, ACP). These initiators It can also be used. The initiator can also be active at a certain stage of the plant's hair, or in plant tissues and organs. Examples of such initiators include (but are not limited to) pollen specificity, embryos Specificity, corn Specificity, cotton fiber specificity, root specificity, seed endosperm specific initiator, etc. In some cases it is also desirable to use an inducible initiator. An inducible initiator is responsible for a specific message. The expression of genes, such as physical stimuli (heat shock genes); light (RUBp carboxylase); hormones (Em), metabolites and urgency. Other transcription and translation factors that function in plants can be used Many plant-specific gene delivery vectors are known in the art. In addition, it is known that in order to make bacterial genes highly expressed in plants, the bacterial genes should be reconstructed so as to be more effectively expressed in the cytoplasm of plants. This type of plant is suitable for reconstructing the bacterial gene before transformation to improve the expression of toxins in the plant. Reasons for the reconstruction • 23- This paper size applies the Chinese National Standard (CNS) A4 specification (210 × 297 mm) ) (Please read the notes on the back before filling this page) Order 509722 A7 B7 V. Description of the invention (21) (Please read * Notes on the back before filling this page) One is the extremely low G + C content of natural bacterial genes (resulting in an abnormally high A + T content). This results in the generation or imitation of control sequences of plant genes known to be rich in A + T. Gene DNA of certain introduced plants The presence of A + T-rich sequences (such as TATA regions usually found in gene promoters) can lead to abnormal expression of these genes. On the other hand, the presence of other regulatory sequences within the transcribed mRNA (such as Polyadenine signal sequences (AAUAAA) or sequences that are complementary to small nuclear RNAs that involve pre-mRNA junctions can cause RNA instability. Therefore, designing a reconstructed bacterial gene (known as a plant adaptation gene is better) is a goal In order to produce a DNA sequence with a high G + C content, a plant gene encoding a metabolic enzyme and similar ones are preferred. Another goal in designing plant adaptation genes is to produce a DNA sequence that not only has a high G + C content, but also modifies the sequence without impeding translation. One example of a plant with a high G + C content is corn. The table below shows the high G + C content in corn. As in corn, G + C content in other plants is also considered high. Type of protein printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs a ° / 〇G + C Range average ° / 〇G + Cb Metabolic enzymes (40) 44.4- 75.3 59. 0 (8.0) Stored protein group I (23) 46 0-51.9 48 · 1 (1.3) Group II (13) 60.4-74. 3 67. 5 (3.2) Group I + II (36) 46. 0-74. 3 55 · 1 (9. 6) c -24- This paper size applies Chinese National Standard (CNS) A4 (210 X 297 mm) 509722 A7 B7 V. Description of the invention (22 Structural protein (18) 48. 6-70.5 63 · 6 (6.7) Regulating protein (5) 57 · 2-68. 9 62.0 (4.9) Uncharacterized proteins (9) 41.5-70 · 3 64.3 (7.2) All proteins (108) 44.4- 75.3 60.8 (5.2) Consumption cooperatives print a. The number in the brackets is the number of various types of genes. The standard deviation in the brackets is b. E. The mixed group average calculated by omitting the total mean. Regarding the information in Table 2, the coding regions of these genes are quoted from GenBank (Release 71). The base composition was calculated using the MacVectorTM (IBI, New Haven, CT) program. The spacer sequence was omitted in the calculation. The first and eleventh group storage protein gene sequences It is distinguished by the obvious difference in its base composition. The plasticity provided by the repetitive nature of the genetic code (that is, certain amino acids are designated by more than one codon), genes of different organisms or biological classes The evolution of the body has caused different uses of repeated codons. This so-called "codon bias" is reflected in the average base composition of the protein coding region. For example, organisms with relatively low G + C content use repeated codons The 3rd position is the codon of A or T, and the higher G + C content uses the 3rd position of the codons of G or C. The presence of less codons in the gene's mRNA is considered to reduce the mRNA The absolute translation rate, especially when the relative content of tRNA corresponding to the less-used codon is relatively low. This is extended by reducing the translation rate by a single less-used codon. Additive. Therefore, it has a high relative content of -25- This paper size is applicable to the Chinese National Standard (CNS) A4 specification (210X 297 mm). Read and read the precautions on this page before filling in this page 509722 A7 B7 V. Invention Description (23) The translation rate of the less codon mRNA is relatively low. This rate is reflected in the low amount of synthesis of the encoded protein. Printed by the Consumer Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs (please read the notes on the back before filling out this page). In order to reconstruct these bacterial genes, the plant's codon preferences are determined. The codon preference is a statistical codon distribution that the plant uses to encode its protein. After deciding on their preferences, the frequency of codon percentages in the genes we are interested in is determined. The plant's preferred codons and the second and third choices of the preferred codons are also determined. The amino acid sequence of the protein of interest is reversed and the resulting nucleic acid sequence encodes the same protein as the natural bacterial gene. The resulting nucleic acid sequence corresponds to the preferred codon of the desired plant. This new sequence analyzes restriction enzyme sites that may have been generated by modification. The identified restriction enzyme sites are further modified by substitution of the second and third preferred codons. Other restriction enzymes within the sequence that may hinder the transcription or translation of the gene of interest are the expression segments: 5 ′ or 3 ′ junctions of spacers, poly A signal or RNA polymerase stop signal. This sequence was further analyzed and modified to reduce the frequency of TA or GC doublets. With the exception of this group, consecutive identical G or C sequences of more than 4 residues prevent transcription of the sequence. Therefore, these consecutive sequences are also modified by substituting codons of the first choice or codons of the next choice. Preferably, these plant adaptive genes contain about 63% of the preferred codons, between about 22% and 37% of the codons of choice, and between 15% and 0% of the third choice of codons, of which the total percentage Is 100%. Best of all, these plant adaptive genes contain about 63% of the preferred codons, at least about 22% of the second codons, about 7.5% of the third codons, and about 7.5% of the fourth codons, of which the total percentage is 100. %. The above-mentioned method can make people familiar with this technique. 26- The paper size is applicable to the Chinese National Standard (CNS) A4 specification (210X 297 mm) 509722 A7 ____ B7 V. Description of the invention (24) A person's modification is foreign to a specific plant Genes so that they can be properly expressed in plants. This method is further shown in applications RCT / US96 / 16582, WO 97/13402, published on April Ⅱ, 1997. Therefore, in order to design plant adaptation genes, the non-repetitive genetic code in the codon preference table established for the DNA sequence of a particular plant for transformation is used, and the amino acid sequence of these toxins is translated into a nucleic acid sequence. The obtained DNA sequence (which is completely homogenized in the use of codons) is further modified to create a DNA sequence which, in addition to having a higher degree of codon ambiguity, also contains strategically placed restriction enzyme recognition sites, The desired salt-based composition and the lack of sequences that may interfere with the transcription of the gene or the translation of the product's mRNA. In theory, if bacterial genes are expressed on plastids, these bacterial genes are easier to express in plants. Therefore, without adapting these genes to plant performance, it may be possible to express bacterial genes in plants and obtain high expression of the protein. See U.S. Patent Nos. 4,762,785, 5,451,513, and 5,545,817, which are incorporated herein by reference. Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs. One of the topics on the commercial development of genetically modified plants is drug resistance management. Of particular concern is the thuringiensis toxin. There are many companies that are commercially developed by the company and have noticed that valence toxins develop resistance. One of the strategies for insect resistance management is to combine these toxins generated from photobacteria with, for example, 10,000 ', Ciba Geigy or other toxins. These compositions can be formulated as sprayable applications or molecular compositions. Plants can be produced by the bacillus gene of insect toxins and other insect toxin genes (such as transformations. European Patent Application No. 0400246A1 describes 20,000 in a plant -27- _ Standard Standard (CNS) A4 Specification ( 21〇x297 ^ F) — 509722 A7 B7 printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs 5. Description of the invention (25) The transformation can be any two genes. The other generates a gene containing more than one anthelmintic gene The method of transplanting plants is to generate two kinds of plants, each of which contains an anthelmintic gene. These plants use traditional plant breeding techniques to backcross to produce a plant containing more than one anthelmintic gene. In addition to gene-transgenic plants that adapt to genes, others re-construct the distribution system of these bacterial genes as they wish. Following the same approach (genetic engineering), it is easy to separate isolated protein toxins and attract insects as food sources. The molecule and the insecticidal active fusion of the toxin can be performed and expressed in bacteria or eukaryotic cells using standard, well-known techniques. In experiments After the chamber is purified, this poison and “built-in” bait can be packaged in a standard trap house. Another distribution system scheme is to incorporate the genetic material of the toxin into the baculovirus vector. Baculovirus Infects specific insect hosts, including those who want to be targeted by such bacilliform toxins. Infectious baculoviruses possessing a structure for the expression of bacillus toxins can be introduced into areas where insects parasitize and killed by poison Infected insects. Transmission of insecticidal properties requires the insertion of a nucleic acid sequence encoding the amino acid sequence of a bacillus toxin into a protein expression vector suitable for the host in which the vector resides. One of the methods of protein nucleic acid sequence is to use the information deduced from the amino acid sequence of toxins (mostly published below) to isolate the natural genetic material of toxins produced by Bacillus bacteria. As described below, the method of purifying these toxin proteins is also Revealed. Using N-terminal amino acid sequence data (published below), we can construct -28- Chinese standards as applicable to paper standards Standard (CNS) A4 (210 X 297 mm) --------- Approval of clothing-(t read the precautions on #v side and then fill out this page} Order 509722 A7 Central Bureau of Standards, Ministry of Economic Affairs Cooperative printing by employees 5. Duplicate description of invention (26) Oligonucleotides complementary to all (or part of) j) NA bases encoding the amino acid at the front end of the toxin. These oligonucleotides can be radioactively labeled and Used as a molecular probe to isolate genetic material from a gene bank that constructs the genetic material isolated from free Bacillus strains. The gene bank can be selected for plastids, cosmids, phages, or phages The library can be transformed into E. coli and screened for toxins generated from the transformed cells using direct analysis of antibodies or insect toxicity induced by the toxin. Because the degenerate genetic code allows a single amino acid to be encoded in DNA through a combination of multiple, three nucleotides, the solution requires the generation of many oligonucleotides. For example, amino acid arginine can By the nucleic acid triplets CGA, CGC, CGG, CGT, AGA, AGG are coded. Since it cannot be used for any of the positions within the toxin gene, it must be borrowed to contain every possible triplet present Oligonucleotides. More than one DNA molecule corresponding to one protein subunit is required to construct a sufficient number of oligonucleotide probes to discover the protein subunits required to cause oral toxicity. The amino acid of the purified protein Sequence, the genetic material responsible for the production of toxins can be isolated and cloned in whole or in part into a performance vector using any technique familiar to those skilled in molecular biology. Although other delivery methods include, but are not limited to, this Aggregation vectors, phage plastids, and phages can also be considered. Typical expression vectors are DNA plastids. In addition to the properties required or desired for plastid replication, such as a replication origin and antibiotics Drug resistance or other forms of selection markers (such as streptomycin aquatic (please read the precautions on the back before filling out this page) • Pack · -Order d

509722 A7 B7 V. Description of the invention (27) In addition to [Stretomyces or bar kiesi of Strep tomyces viridochromogenes], protein expression vectors usually require an expression cassette, which is incorporated into a gene transcription And the cis_acting sequence required for translation. These cis-acting sequences required for performance in prokaryotes are different from those required in eukaryotes and plants. A eukaryotic expression cassette requires a transcription initiator located upstream (5 ') of a gene of interest, a transcription termination region (such as a poly A join site), and a first codon upstream of a gene of interest. Ribosome binding site. In bacterial cells, a useful transcription starter that can be included in the body is the T7 RNA polymerase binding initiator. The aforementioned promoters herein are known to effectively promote mRNA transcription. Upstream of the gene of interest, the vector also contains a nucleotide sequence encoding a signal sequence that is known to direct a covalently linked protein to a specific part of the host cell, such as the cell surface. Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs --------- 0 ^ ---- ♦ t (Please read the precautions on the back before filling this page) Insect virus (or baculovirus) is known to infect And affect some insects. The effects of these viruses on insects are slow, and they do not block insect feeding. Therefore, viruses are not considered to be effective pest control agents. Integrating the Bacillus toxin gene into a baculovirus can provide an effective method for transmitting these toxins and increase the lethality of the virus. In addition, because different baculoviruses are specific to different insects, it is possible to selectively attack specific pests with specific toxins. A particularly useful vector for these toxin genes is a nuclear polyhedrosis virus. The delivery vector using this virus has been described, and now it has become the first choice for delivery of foreign genes to insects. -30- This paper size applies to China National Standard (CNS) A4 (210X29? Mm) 509722 Central Bureau of Standards, Ministry of Economic Affairs A7 B7 printed by Employee Consumer Cooperative V. Invention Description (28). The virus-toxin gene recombinant can be constructed by oral transmission. Baculoviruses usually infect insects through the midgut intestinal mucosa. The toxin gene inserted behind the starter of a powerful virus outer protein can express and quickly kill infected insects. In addition to insect vectors, baculovirus, or genetically modified plant distribution systems for use with the protein toxins of the present invention, these proteins can be encapsulated using a thurium coating technique such as, but not limited to, a U.S. patent Nos. 4, 695, 455, 4, 695, 462, and 4, 861, 595, all of which are incorporated herein by reference. Another distribution system for the protein toxins of the present invention is a formulation that adds the protein to a bait matrix, which can be used on ground or underground insect bait locations. Examples of this technology include, but are not limited to, PCT Patent Application No. WO 93/23998, which is incorporated herein by reference. As described above, when expressed in an unnatural host, it is necessary to modify the sequence encoding the protein, because the codon preference of other hosts may be different from that of Corynebacterium. If so, the translation in a new host will be rather inefficient unless the coding sequence is modified. In addition, it is also desirable to modify these amino acid sequences to avoid inhibitory cross-reflection with the protein of the new host or to enhance the insecticidal properties of the protein in the new host. A genetically modified toxin gene can encode a toxin that can exhibit, enhance or reduce toxicity, change insect resistance development, and change stability or specificity of the modified target species. In addition to the genes encoding the toxins of these toxins, the scope of this invention (21GX297:) ------ I -------- 0 ^ — 丨 (A first read the precautions on the back before (Fill in this page) Order 509722 Printed by A7 B7, Consumer Cooperative of the Central Standards Bureau, Ministry of Economic Affairs 5. The description of the invention (29) is extended to include related nucleic acid sequences, which encode amino acid organisms that are homogeneous with these toxin proteins The toxic effects of polymers and retention of Bacillus protein in insect species after oral administration. For example, "the toxins used in the present invention appear to inhibit larval feeding first" and then die. By manipulating the nucleic acid sequence of the Bacillus toxin or its control sequence, the genetic engineer can place the toxin gene in the plant to regulate its ability or mode of action, such as maintaining the feed-inhibiting activity to remove absolute toxicity to larvae. This change allows the transgenic plant to survive to harvest, without causing unnecessary dramatic effects on the ecosystem and destroying all insects. All such modifications to the gene encoding the toxin or the protein encoded by the gene are within the scope of the invention. Other foreseeable modifications of the nucleic acid include the addition of targeting sequences to direct the toxin to specific parts of the insect larva to increase its efficiency. The W-14 strain (ATCC 55397, 43948, 43949, 43950, 43951, 43952) has been deposited in the American Type Culture Collection (12301, ParklawnDrive, Rockville, MD 20852 USA). The amino acid and nucleotide sequence information of W-14 natural toxin (ATCC55397) is shown below. Genomic DNA isolation from bacterial hosts for these toxins is also exemplified herein. Other strains identified in this document have been deposited with the United States Department of Agriculture (1815 North University Drive, Peoria, IL 61604). Standards and molecular biology techniques are followed and taught in the patent specification herein. Other materials can be found in Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989), Molecular cloning, A Laboratory Manual, Cold Spring Harbor Press; Current Protocols 32- ) A4 size (210 X 297 mm) (Please read the notes on the back before filling out this page) i. Order. Printed by the Consumers Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs 509722 A7 B7 V. Description of the invention (3〇) in Molecular Biology, ed. FM Ausubel et al., (1997), both of which are incorporated herein by reference. The following abbreviations are common in these examples: Tris is hydroxymethylaminomethanol; SDS is dodecyl sodium sulfate; EDTA is ethylene glycol diaminotetraacetic acid; IPTG is isopropylthio-B-galactoside , X-gal is 5-mo-4-qi-3-, galactose, CTAB is cetyltrimethylammonium bromide; kbp is a thousand-salt-based pair; I is adenosine, cell carcinoma, guanosine, thymine, and 2'-deoxynucleoside 5'-triphosphate of inosine; ATP is adenosine 5 'triphosphate. Example 1 Demonstration of purified toxin from Corynebacterium radians and toxicity of purified toxin after oral administration The insecticidal protein toxin of the present invention was purified from strain W-14 of Corynebacterium radiata (ATCC Accession No. 55397). Mass culture of Corynebacterium radians was maintained in a Petri dish containing 2% of Proteose Peptone No. 3; PP3, Difco Laboratories, Detroit MI in 1.5% agar. Succession every week. Colonies in the original form of the bacteria were inoculated into 200 ml of a 1-liter triangular cone bottle containing 0.5% — PP3 meat stearic acid polysorbate sorbitan (Tween60, Sigma Chemical Company, St. Loise, M0) In the soup. The broth culture was grown on a convoluted shaker at 30 GC for 72 hours. The toxin protein can be recovered from the growing culture regardless of the presence or absence of Tween; however, the lack of Tween can affect the form of growing bacteria and the protein image produced by the bacteria. In the absence of Tween, a different transformation occurs, and the molecular weight of at least one identified toxin subunit changes from about 200kDa -33- This paper size applies the Chinese National Standard (CNS) A4 specification (210X297 mm) I ---- --- 0 ^-(t read first, note on the back, then fill out this page) Order d 509722 A7 B7 V. Description of the invention (31) is 185kDa. The 72-hour culture was centrifuged at 10,000 xg for 30 minutes to remove cells and debris. The supernatant containing the insecticidal activity was decanted, and an appropriate amount of 1.0 M K2HP〇4 was added to make it 50 mM K2HP〇4. The pH was adjusted to 8.6 by adding potassium hydroxide. The supernatant fraction was then mixed with DEAE-Sephacel (Pharmacia LKB Bio-technology) which had been equilibrated with 50 mM K2HP04. The toxic activity is adsorbed to the DEAE resin. This mixture was then poured into a 2. 6x40cni column 'and washed with 50mM K2HP04 at a flow rate of 30ml / hr at room temperature until the effluent reached a stable baseline of UV absorbance at 280nm. The column was then washed with 150 mM KC1 until the effluent reached a stable baseline of 280 nm again. Finally the column was washed with 300 mM KC1 and the fractions were collected. Printed by the Consumers' Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (A read ^: Precautions before filling in this purchase) The separated part containing the toxin was collected and filtered and sterilized by a membrane filter with a pore size of 0.2 microns. The toxin was then concentrated using an ultrafiltration membrane (Centriprep 100, Amicon Division-W.R. Grace and Company) with a molecular weight cutoff of 100 kDa at 4GC and equilibrated to 100 mM K04. A 3 ml sample of one of the toxin concentrates was applied to the top of a 6x95 cm Sephacry 1 S-400 HR gel transition tube column (Pharmacia LKB Biothechnology). The elution buffer was 100 mM KP04, pH 6.9, and the flow rate was 17 ml / hr under 4GC. The effluent was monitored at 280 nm. Separate fractions were collected and tested for toxic activity. Chromatographic analysis of the toxicity of the separated fractions was tested using a biological analysis of tobacco hornworm (you; sezia) larvae. The separated part is directly added to the food of this insect (Gypsy moth wheat germ feed, ICN Biochemical Division-ICN Biochemical, _____- 34- This paper size applies to China National Standard (CNS) A4 (210X297 mm) ~ Central Standard of the Ministry of Economy Printed by the Bureau's Consumer Cooperatives 509722 A7 B7 V. Invention Description (32)

Inc.) or a 5μ1 sample was injected into the heart embryo cavity and injected into the first protopod of the 4th or 5th instar larva with a 30 gauge needle. The weight of each larva in an experimental group was recorded every 24 hours. If the insect stops eating or dies within a few days after ingestion of the insect feed after treatment, or if it dies within 24 hours after the injection of a separate part, its toxicity can be inferred. The toxic fraction was collected and concentrated using Centirprep-III, and then 7.5111111 and 60 (: 11 ^ 31 [-0 £ 10-400 3 ~ gel permeation column, 100mM potassium phosphate pH 6.9 elution buffer, Passed at 0.4 ml / min and analyzed by HPLC. This analysis showed that the toxin protein was contained in a single sharp spike that elutes from the column within a retention time of about 33.6 minutes. This retention time (Retention time) corresponds to an estimated molecular weight of 1,000 kDa. The spike fraction was collected for further purification and the fraction that did not contain this protein was discarded. The spike eluted from the HPLC absorbed UV light at 218 nm and 280 nm but Does not absorb 405nm. The absorbance at 405nm is a property of an xenorhabdin antibiotic compound. The collected peak-separated fraction was electrophoresed on a non-denaturing agarose gel (Metaphor Agarose, FMC Bio-Products). The protein complex is present in the spikes. The spiked substance buffered with 50mM Tris_HCl, ρΗ7.0 · 0 is stored in 1.5% agarose stacking gel buffered with 100mM Tris_HCl, ρΗ7.0 · 0 and 200mM Tris-boric acid. ρΗ8.3 Buffered 1.9% agarose resolving gel under standard buffer conditions (cathode buffer 1M Tris-HCl, pH8.3; anode buffer 0.025M Tris, 0.192M glycine) It was separated. The gel was electrophoresed at a constant current of 13 mA under 15GC until the phenol red tracking dye reached the end of the gel. Using -35- This paper size is in accordance with Chinese National Standard (CNS) A4 specification (2! 〇'〆297 mm ) (Please read the precautions on the back before filling this page) Γ ⑽722 ⑽722 Printed by the Consumers' Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs A7 —----------- B7 V. Invention 33) ~~~ -Bituminous sapphire blue staining shows 2 protein bands on this agarose gel. The slower moving band is called "the first protein band, and the faster moving band 2 is called" the second protein band. " The amounts of the two protein bands are approximately equal. The ancient Marsh-stained agarose gel was used as an index to correctly cut the two protein bands from the unstained portions of the gels. Bands containing these proteins < Cut pieces are soaked and add a small amount of sterile water. A protein-free portion of the gel was also excised and treated in the same manner as the protein-containing gel pieces as a control group. The protein was eluted at 100 volts (constant voltage) by electricity for 2 hours. From the gel pieces, 100 mM Tris-boronic acid, pH 8.3 was recovered. Optionally, protein can be added by adding 50

Tris-HC1, ρΗ7 · 0 was then passively eluted from the gel pieces by incubation at 30 GC for 16 hours. This allows the protein to diffuse from the gel into the buffer and then be collected. The results of an insect toxicity test using HPLC-purified toxins (33.6 min spike) and agarose gel-purified toxins showed the toxicity of the extract. Injection of this HPLC purified protein 1.5 μδ will be fatal within 24 hours. By passive elution or electroelution, injection of both the first and second protein bands recovered from the agarose gel can be fatal. The estimated protein concentration of these samples was lower than 50ng per larva. Comparison of weight gain and mortality between the two groups injected with the first or second protein band showed that the first protein band was more toxic by injection. When HPLC-purified toxins were added to larval food at a concentration of 7.5 pg per larva, the larval weight was stagnated (24 larvae tested). The larvae initially ate, but started to eat a small amount of food treated with toxins. -36- Exhausted paper Huainfandu General Ningguo National Standard (CNS) A4 specification (210X297 public celebration) (Please read the back Note: Please fill in this page again) | Binding · Bookmark -n ϋϋ 509722 Printed by A7 B7, Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 5. Description of the invention (34) Present the pathological symptoms induced by the toxin and stop feeding. The insect's feces fade and most larvae show symptoms of diarrhea. Adding multiple 5 pg toxin doses to food during the 7-day period caused significant insect death. The agarose-isolated first protein band significantly inhibited larval weight gain at a dose of 200012 per larva. Larvae that ingested the second protein band at similar concentrations were not inhibited and gained the same rate as the control larvae. Twelve larvae were fed with eluted protein, and 45 larvae were fed with protein-containing agarose pieces. These two sets of data show that the first protein band is orally toxic to tobacco hornworm. The second protein band in the experiment did not appear to be toxic to the tobacco hornworm. Further analysis of the i and 2 protein bands under denaturing conditions by SDS-PAGE revealed that each band consists of multiple smaller protein subunits. Proteins were stained with bituminous sapphire blue, followed by silver staining for maximum sensitivity. The protein subunits in the two bands are very similar. The first protein band contains eight protein subunits of 25.1, 56.2., 60.8, 65.6, 166, 171, 184, and 208kDa. The second protein band has the same image, except for 25.1, 60.8, and 65.61 ^) & proteins. The 56.2, 608, 656 and 184 kDa proteins are present in the protein complex at approximately the same concentration, and the total protein content of the complex is above 80%. Toxins purified by natural HPLC were further characterized as follows. The toxin is antipyretic. It is heated to 60GC for 15 minutes, and the toxin loses its ability to cause death or inhibit weight gain after injection or feeding of tobacco hornworm larvae. These analyses are designed to detect lipase, C-type phospholipase, nuclease or red blood cell hemolytic activity and to detect purified toxins. None of these activities exist -37- --------- • Pack II * ^ (Please read the notes on the back before filling this page) * 1Τ 509722 A7 B7 V. Description of the invention (35). Antibiotic band inhibition analysis was also performed. The purified toxin failed to inhibit the growth of Gram-negative or positive bacteria, yeast or filamentous fungi, suggesting that the toxin is not an isoformin antibiotic. This natural HPLC purified toxin was tested for its ability to kill insects other than tobacco hornworms. Table 3 lists the insects killed by luminescent Bacillus toxin purified by HPLC in this study. Table 3 Insects killed by luminescent bacillus toxins JL genus and species name Route of administration Tobacco hornworm Lepidoptera Manduca sexta Oral injection, Oryzae coleoptera Tenebrio molitor Oral pharaohs Hymenoptera Monomorium ph a roan is Oral German roach Further features of Oral, injectable mosquitoes, Aedes aegypti, and high-molecular-weight toxin complexes of Blete 11a germanica have been printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (please read the precautions on the back before filling out this page). In the analysis, the toxin-protein complex was further characterized by W-14 growth medium. The culture conditions are the same as those described above for the initial purification through an S-400 HR column. After separating the polymer toxin complex from the S-400 HR column separation section, the toxic separation section was equilibrated with 10 mM Tris-HC1, ρΗ8.6, and concentrated in a Centriplus-100 (Amicon) concentrator. The protein toxin complex was then applied to a weak ion exchange (WAX) column (Vydac 301VPH575; Hesparia, CA) at a flow rate of 5 ml / min. These proteins are eluted with a linear gasification clock gradient. The -38-This paper is in accordance with the Chinese National Standard (CNS) A4 (210 X 297 mm) 509722 Α7 Β7 5. Description of the invention (36) The gradient is at 10mM Tris 0-250 mM KC1 in HC1, ρΗ8 · 6 for 50 minutes. Eight protein spikes were measured by absorption at 280 nm. All the separated fractions eluted from the HPLC column were subjected to the analysis of young southern corn rootworm (and / aAn? &Ca; ca undecimpunctata Aourarcij ·, SCR) young A and tobacco Xiao A (Manduca sex ta, 1). THW was bred with Gypsy Moth Wheat Germ Feed (ICN) at 25GC, 16 hours light and 8 hours of darkness. SCR was Southern Corn Rootworm larva insect feed (Bioserv) at 25GC, 16 hours light / 8 hours of darkness Printed by the Consumer Cooperatives of the Central Bureau of Standards of the Ministry of Economic Affairs (A reads the precautions on side t before filling out this page) The highest mortality rates of SCR and THW appear at the 6th peak, which was eluted with ca.ll2mM to 132mM KC1. The SDS-PAGE analysis of the 6th peak showed 170kDa, 66kDa, 63kDa, 59.5kDa, and 31kDa superior peptides. The 6th peak protein separated part against TcaAu-syn, TcaAHi-syn, TcaBii-syn, TcaC-syn, and TcbAii The multi-source antibody mixture of the -syn peptide (see Example 21) and the single-source antibody C5F2 against TcbAiii-syn peptide were subjected to Western rupture analysis. The sixth peak contains 170 kDa, 90 kDa, 66 kDa, 5 kDa, and 31 kDa immune response bands. Its points It is similar to the predicted size of TcaC (166kDa), TcaAH + TcaAUi (92kDa), TcaAin (66kDa), TcaBH (60kDa) and TcaAu (25kDa). As described in this article, the 6th by natural agarose gel electrophoresis analysis The spike moves in a single band, which has the same motility as the first protein band. The protein concentration of the purified 6-spot toxin protein was determined using BCA reagent (Pierce). The protein was made with 10mM Tris, ρΗ8.6 The dilution is used for feed biological analysis. After 240 hours, feed biological-39- This paper size applies to Chinese National Standard (CNS) A4 specification (210X 297 mm) 509722 A7 B7 V. Description of invention (37) All The newborn larvae that ingested 450ng or more of the 6th spike protein isolate died. The larvae group that consumed 90ng of the same isolate had a 40% mortality rate. After 240 hours, 90ng and 20ng of the 6th spike protein isolate were ingested. The survivors were ca. 10% and 70% of the control weight, respectively. Example 2 Practicality and toxicity of the insecticide practicality of G. luminifera was further imparted. G. luminescence (W-14 strain) The culture broth was prepared as follows. The production medium was printed in Mill iQ® deionized water with No. 3 Bacto protein glycoprotein gland to the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (please read the precautions on the back before filling this page) 2 % (PP3, Difco Laboratories, Detroit, Michigan). The culture flask containing 175 ml of the culture medium was placed in a 500 ml tribaf fled flask with a Delong neck, sealed with a Kaput, and sterilized with 250GF for 20 minutes. A 500 ml production culture bottle was placed in an 8 liter three-way flask with a Delong neck, and was sealed with a Shin-etsu silicone foam plug. These were sterilized at 250 QF for 20 minutes. This strain culture was grown at 280C at 150 rpm in a rotary spinner incubator with a 2-inch stroke. After 16 hours of growth, 1% of the strain culture was placed in a generation flask, which allowed growth for 24 hours before harvest. The toxin appears to be growing in the log phase. The microbial broth was transferred to a 1 liter centrifuge bottle and the cell bioagglomerates were pelleted (at 2500 RPM at 40 ° C for 30 minutes [RCF ca. 1600] HG-4L spinner RC3 Sorval centrifuge, Dupont, Wilmington, DE ). The first-generation broth was cooled at 40 ° C for 8-16 hours and centrifuged for at least 2 hours (conditions as before) to further clarify the broth with the presumed mucopolysaccharide. (An optional treatment method combines two steps and involves the use of a 16-hour clarification centrifugation. The same conditions as __ -40- This paper size applies to China National Standard (CNS) A4 (2i0x297 mm). Economic Printed by the Consumer Standards Cooperative of the Ministry of Standards of the People's Republic of China 509722 A7 B7 V. Description of the Invention (38)) This broth was then stored at 40 ° C before bioanalysis or filtration. The bacillus broth culture broth and the protein toxin purified from this broth exhibit anti-activity against a variety of insects (mortality and / or growth inhibition, and reduced adult paralysis). More specifically, this activity is effective against corn rootworms (larvae and adults), Colorado horsefly potato beetles and grassworms, which are members of the order Coleoptera. Other members of the Coleoptera include a beetle, a pollen beetle, a flea beetle, a seed beetle, and a weevil. The activity has also been shown to be able to fight against Astrodia, a member of the homoptera order. Other members of the order Homoptera include plant hoppers, pear wood, apple flukes, scale insects, white flies, blowworms, and many host-specific species of aphids. The toxin and the purified isolated part are also active against beet burglar moth, cabbage worm, brown sugar moth, tobacco budworm, European corn perforator and apple moth, which are members of Hymenoptera. Other typical members of this order are tapeworms, Indian rice moths, leaf rollers, cabbage worms, cotton moths, sleeping bagworms, oriental tent caterpillars, lawn networms, and autumn night moths. The activity is also seen against larvae against fruit flies and mosquitoes, which are members of the order Diptera. Other members of the Diptera order are pea maggots, carrot flies, cabbage roots, grape root flies, onion ropes, long-legged ropes, home ropes, and different mosquito species. The activity is also seen against carpenter ants and Argentine ants, including fire ants, scented house ants and small black ants. The broth / separation portion can be used to reduce the population of insects and used in a method of suppressing an insect population. The method may include applying an effective insect-inactivating amount of the active substance to an area of the insect. The results are published in Table 4. (Please read the precautions on the back before filling in this page} ’Order The following test against the activity of corn rootworm larvae.

509722 A7 B7 V. Description of the invention (39) (Please read the notes on the back before filling out this page) Broth (遽 sterilized 'without cells) or purified HPLC separation part is directly added to the surface of 0.25ml artificial feed ( (Approximately 1.5 cm2), adding 30 μl portions, and diluting with control medium or 10 mM sodium phosphate buffer, ρΗ7.0 The feed tray was air-dried in a sterile operation box, and the wells were placed into a single-stage Diabrotica undecimpunctata howardi (Southern Corn Rootworm, SCR; its self-sterilized egg hatching), grown on artificial feed SCR second instar A, IDiabrotica virgifera virgifera {Western corn rootworm, WCR; it is raised on corn seedlings grown on Metromix®). Second instar larvae were weighed before being placed on the feed. The culture plates were sealed and placed in a humidified growth box for a period of 27GC (appropriate for SCR newborns and adults on the 4th, WCR larvae on 2-5th, and second-instar SCR larvae on 7-14). Mortality and weight measurements are recorded as shown. In general, 16 insects were used for each treatment in all studies. The mortality rate of the control group is as follows: less than 5% of newborn larvae and 5% of adult larvae. Printed by the Consumer Standards Cooperative of the Central Bureau of Standards of the Ministry of Economics The activity against Colorado potato beetles was tested as follows. The surface of the bacilliform culture broth or control medium was added to a 1.5 ml standard artificial feed (approximately 2.0 cm2) fixed in a well of a 24-well tissue culture plate. Each well was added with 50 μl of the treatment and air-dried. Individual second-in-class Colorado potato crusts (XLeptinotarsa decemlineata, CW) were used on this feed, and mortality was recorded after 4 days. Ten insects were used for each treatment in all studies. Control group mortality was 3.3%. The activity against Japanese tapeworm larvae and adults was tested as follows. Collect turf beetle larvae (eighth;? /// 3 / apow / ca, 2nd to 3rd instar) from the infected grassland, and add juniper tablets as soil for feed in the laboratory / ___- 42- This paper size applies to Chinese National Standard (CNS) A4 (210 X 297 mm). Printed by the Consumer Cooperatives of the Central Bureau of Standards of the Ministry of Economic Affairs. 509722 A7 B7. 5. Description of the invention (4) Peat mixture breeding. Lawn beetles are trapped locally with pheromone, maple leaves are used as food in the laboratory, and they are kept in plastic containers. Then add undiluted bacillus broth culture broth or control medium to corn rootworm artificial feed (30μ1 / 1. 54cm2 'adults') or carrot slices (larvae). Both stages are individually housed in a feeding well and observed Its mortality and feeding situation. In both cases, a significant reduction in feed intake (and fecal production) was observed. The activity against mosquito larvae was tested as follows. The analysis was performed in a 96-well microtiter plate. Each well contained 200 μl of an aqueous solution (bacteriform culture broth, control medium or water) and about 20 1-day-old larvae (Egyptian mosquito). There are 6 wells for each treatment. These results were interpreted 2 hours after joining and remained unchanged during the 3-day observation period. No death occurred in the control group. The activity against fruit rope was tested as follows. The purchased fruit iron calendar medium was prepared by using 50% dry medium and 50% water, a control medium or a bare rod-shaped culture liquid for broth. This is made by placing 3.0 ml of dry medium in each rearing vial of each treatment and adding 8.0 ml of a suitable liquid ^ 10 sisters of the last-age fruit rope are then added to each bottle . The bottles were placed on a laboratory table under fluorescent light at room temperature. Pupae and adults were counted 3, 7 and 10 days after exposure. Relative to water or control medium, the addition of bacilliform culture broth to the feed medium caused a slight (Π%) but insignificant reduction in the hatching of fruit fly pupae on the 10th day (3% reduction in the control medium). The activity against Astrodia spp. Was tested as follows. The feeding analysis for Astrodia spp. Is designed in the absence of other foreign ____ · 43- This paper size is applicable to the Chinese National Standard (CNS) Α4 specification (210X297 mm) — '(Please read the precautions on the back before filling in this page)

-、 1T 509722 Printed by the Consumer Cooperatives of the Central Bureau of Standards of the Ministry of Economic Affairs A7 B7 V. Description of the invention (41) The active substance was ingested under contact. The active substance / "food solution grain trough" is made by drilling two holes in the center of the bottom of a 35x10mm petri dish. A 2-inch 1: 1 inch square Paraf ilin M® is placed on the petri dish and the The clothes were fixed. Then put about 7 jumping worms in a 1 ounce plastic cup. "Place the food container on the cup (Parafilm down). Then add the test solution into the container through the hole. Use the undiluted polished rod shape. In the experiment conducted by the bacterial culture broth, the broth and the control medium were dialyzed against water to reduce the mortality rate of the control group. In the second day, the mortality rate of the control group was published as 26.5%. In the experiment using the purified fraction, 5 The final concentration of% sucrose was used in all treatments to improve the survival rate of Spodoptera exigua. The analysis was performed in a 28GC, 70% relative humidity, 16/8 photoperiod incubator. The analysis was evaluated at 72 hours Mortality in the control group was 5.5%. The activity against the Argentine ants was tested as follows. 10 ml of the culture broth of Bacillus spp., Control medium or 1.5 ml of water were added to 2.0 ml transparent glass bottles. The bottle is sealed with a piece soaked with a suitable treatment Dental cotton (cottondental wick). Each bottle is placed in a 60 乂 16111111 petri dish with 8 to 12 Argentine ant adult ants (2 // 7 Indian / Dead /? Friends to talk about 77). Each Three treatments were replicated. The bioanalytical disc was placed on an experimental table under fluorescent light at room temperature. The mortality was determined after 5 days of exposure. The mortality rate in the control group was 240/00. The activity against carpenter ants was as follows. Black carpenter ants The work sacrifice (Ca / 75/7/757 / IV was collected from the tree of the DowElanco industry in Indianapolis, Indiana. The tests performed on the culture broth of Bacillus spp. Are as follows. Each plastic bioanalytical container ( 7 ι / 8 ,, χ 3 ,,) -44 · This paper is again applicable to China National Standard (CNS) Α4 size (210 X Norm) (Please read the precautions on the back before filling this page) ~ | ^ Install ·, fill in the items too

、 1T 509722 A7 B7 printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs ----_ 5. Description of the invention (42) Put 15 worker ants, 1 paper hiding place and 10ml in a plastic shallow cup Broth or control medium. A cotton lamp core feeds the treatment to the ants through a hole in the lid of the cup. All treatments contained 5% sucrose. Bioanalysis was performed in room 1 in the dark and evaluated on day 19. The mortality rate in the control group was ⑽. The analysis of the purified separated part by dispersing artificial ant feed was mixed the treated product (purified separated part or control solution) at a ratio of 0.2 ml of treated material to 2.0 g of feed in a plastic test tube. The final protein concentration of the purified fraction is less than per gram of feed. In each group, only one tank, one water source, a hiding place, and the treated feed were placed in a sealed plastic container and raised in a 2'70 ° dark tide fishing incubator. Mortality was recorded on the 10th day. There was no death in the control group. The activity against different Lepidoptera larvae was tested as follows. The pull culture broth or purified isolated part of Bacillus bacillus was directly added to the surface of the quasi artificial material (approximately 1.5 cm2) of 0.25 m, and the added part was 30 μ1, and the control medium or l〇 mM sodium phosphate buffer, pH 7 · 0 dilution. The feed tray was air-dried in a "sterile" operation box, and the wells were placed into a single newborn larva. The eggs of European corn perforator (such as with cornworm and earworm; and zea) were supplied from commercial sources and hatched in Indoor. And beet burglars (E. chinensis), Korean cabbage ruler i% iTrichoplusian〇, 'l ^% ^ AUIeliothisviresceiis), black elephant weave {Lasoeyresia pomonel la), and black and green moth ^ (Agrotis / ;? 幻 7 〇 / 7) The larvae are supplied from the inside. After the larvae are added, the feeding trays are sealed, placed in a humidified growth box, and maintained in the dark for an appropriate period of 270C. Determination of mortality and weight The broth culture broth is 5-7 __- 45 -___ This paper size is applicable to China National Standard (CNS) A4 (210X297 mm) (Please read the precautions on the back before filling this page}. Packing ·

1T.4 509722 V. Description of the invention (43: The purified fraction is 4-7 days "—In general, 16 insects per treatment were used in all studies. Range of mortality in the control group: control selection Newborn larvae 4-12.5%, phosphate buffer solution is below. —Pulmonary bacillus (W-14 strain) culture broth. Pure purity = effect of 生长 on mortality and growth inhibition of different insect orders / species Ministry of Economic Affairs Printed isolated part of insect order / seed broth by Central Standards Bureau Consumer Cooperatives% Mort. [% GI% Mort.% GI nt southern / adult 45 nt nt nt western / second instar larva na 35 nt nt Colorado horse cockroach 93 nt nt nt worm, second instar larva turf tapeworm second instar larva na af nt nt adult na af nt nt diptera fruit fly (Adult hatching) 17 nt nt nt Mosquito larva 100 na nt nt Homoptera stellate worm 96.5 na 100 na Hymenoptera Argentina ant 75 na nt na Carpenter ant 71 na 100 na Lepidoptera beet armyworm 12.5 36 .75 41.4 Brown Moth nt nt 0 71.2 Belle Ruler 筻 nt nt 21.9 66.8 Apple moth nt nt 6. 25 45. 9 Corn cotton fluke 56.3 94.2 97.9 na European corn perforator 96. 7 98.4 100 na Tobacco budworm 13.5 52.5 19.4 85.6 ------- -^ — (T first read the notes on the back before filling this page)

Mort · = mortality, G.I. = growth inhibition. -46- This paper size applies to China National Standards (CNS) A4 (210X 297 mm) Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 509722 A7 B7 V. Description of the invention (44) na = No applicability 'nt = Untested 'af = Food Rejection Example 3 Insecticide is applied to soil. The culture broth of Corynebacterium radians (W-14 strain) showed that it had a direct effect when applied directly to soil or a soil mixture (Metromix®). Active against corn rootworm. The activity tests against SCR and WCR in newborn Metromix® are as follows (Table 5). The test was performed using a maize seedling (United Agriseeds CL614 variety) that germinated on a damp filter paper for 6 days in the light. After the roots are approximately 3-6 cm long, a single corn kernel / plant is planted in a 591 ml clear plastic cup with 50 gm of dried Metromix®. Twelve newborn SCRs or WCRs were then placed directly on the roots of the plants and covered with Metromix®. After the insects were added, the corn seedlings were then saturated with a 50 ml total volume of the diluted broth solution. After soaking, the cups were sealed and placed at room temperature for 7 days in a bright place. The corn seedlings were finally washed to remove all Metromix®, the roots were cut and weighed. Activity was estimated as the percentage of corn root residues relative to control plants and leaf damage due to feeding. Leaf damage was assessed visually and estimated as-, +, ++ or +++. -Means no damage, +++ means severe damage. The activity test against the new SCR in the soil is as follows (Table 6). The test was performed using a corn seedling (United Agriseeds CL614 variety) that germinated on a damp filter paper for 6 days in the light. After the root is about 3-6 cm long, a single corn kernel / plant is planted in a 591 ml transparent plastic cup with 150 gm taken from the soil where Lebanon, Indiana had planted corn last year. This soil has not previously been treated with insecticides. Twelve newborn SCRs were then placed directly on the roots of the seedlings and covered with soil. After the insects were added, the corn seedlings were then saturated with a 50 ml total volume of the diluted broth solution. -47- This paper size is in accordance with Chinese National Standard (CNS) A4 specification (2 丨 0'〆297mm) --------- ^-pack-(t read the precautions on the back of Μ before filling This page), 1T d 509722 A7 B7 V. Description of the invention (45) After soaking, the unsealed cups were cultivated in a high relative humidity box (80%) of 78GF. The corn seedlings were finally washed to remove all soil, the roots were cut and weighed. Activity was estimated as the percentage of corn root residues relative to control plants and leaf damage due to feeding. Leaf damage was assessed visually and estimated as-, +, ++ or +++. One means no damage and +++ means severe damage. ^ 5 The effect of parasitic maggots inoculated by the parasitic worm broth (W-14 strain) on parasitic larvae. Metromix® treatment of larval leaf damage to root weight (g)% Southern corn rootworm (please read first Note on the back, please fill in this page again) One pack · — Medium (2.0% ν / ν)-Broth (6.25% ν / ν)-Water + Medium (2.0 ° /. Ν / ν) + Meat Soup (6.25% ν / ν) + Western corn rootworm water, one medium (2.0% ν / ν)-water + medium (2.0% ν / ν) +-0.4916 + 0.023-0.4416 ± 0.029-0.4641 ± 0.081 +++ 0. 1410 ± 0.006 +++ 0.1345 ± 0. 028-0.4830 ± 0. 031-0.444610. 019-0 · 4069 ± 0 · 026-0 · 2202 ± 0 · 015-0.3879 ± 0. 013 7 4 ο ο. · 4 0 0 8 0 0 1i IX 2 3 Ι ο ο 9 5 ο ο 4 9 11 11 Funeral 6 The brilliance of light-emitting Bacillus spp. (W-14 strain) culture broth is soaked after parasitization. Effects of Southern Yulai rootworm larvae · Printed processing broth (50% v / v) Broth (50% v / v) Broth (50% v / v) Broth (+) Root weight (g) 0 2148 ± 0. 014 0 · 2260 ± 0 · 016 0 · 0916 ± 0. 009 0. 2428 ± 0. 032% 0 3 3 3 0 0 4 1 11 1 The activity of the culture broth of Corynebacterium radiata (W-14 strain) against second-instar lawn beetle larvae in Metromix® is shown in the tests performed below -48-_ This paper size applies to Chinese national standards (CNS) A4 specification (21〇 < 297g t) 509722 A7 B7 V. Description of the invention (46) (Table 7). Approximately 50 gm of dry Metromix® was added to a 591 ml clear plastic cup. The Metromix® was then saturated with a 50 ml total volume of diluted bare bacillus broth solution. The crude broth is diluted with water or 25ml of crude broth is added to 25ml of water to make 50% of the total volume of 500ml broth. A 1% solution of No. 3 protein glycoprotein gland (PP3) (which is a 50% dilution of normal medium concentration) was used as a broth control group. After soaking, 5 second-instar lawn larvae were placed on the Yurun's Metromix®. Healthy lawn tapeworm larvae quickly penetrate Metromix®. Those who did not drill within 1 hour were removed and replaced with fresh larvae. The cups were sealed and placed in a dark 28QC incubator. After 7 days, the larvae were removed from Metromix® and their mortality was recorded. Activity was evaluated as a percentage of death relative to the control group. Table 7 The effect of Mushroom beetle larvae larvae (Metromix®) after infiltration of the culture broth of W. luminescens (W-14 strain) before parasitization rate 151920 death 7/2/7 / death 11 mortality% 47 63 85 Processed water control medium (1.0% w / v) Broth (50 liters v / v) * Example 4 expressed as the ratio of dead / surviving larvae 4 Application of insecticides to leaves Application Printed by the Consumers' Cooperative of the Central Standards Bureau, Ministry of Economic Affairs (Please read the notes on the back before filling this page.) The activity of the bacilliform broth against European corn perforator is shown when the broth is directly applied to the surface of corn leaves (Table 8). In these analyses, the bacilliform broth was diluted 100-fold with the medium and applied to the surface of the cut corn leaves by hand at a ratio of about 6.0 μΐ / cm2 of the leaf surface. The leaves were air-dried and cut into equal square pieces of approximately 2x2 inches. The leaves are rolled up and secured with paper clips and placed in a 1 oz plastic shallow cup. The shallow cup _-49- This paper size is in accordance with Chinese National Standard (CNS) A4 specification (210X 297 mm) 509722 A7 B7 5. Description of the invention (47) There is 0.25 inch 2% agar at the bottom to provide moisturization. Twelve newborn European maize perforators were then placed on the rolled leaves and the cups were sealed. After 5 days of incubation in the darkness under 28GC, the larvae of these samples that were damaged due to feeding were recorded. Table 8 Effect of light-emitting Bacillus spp. (W-14 strain) culture broth on parasitic corn leaves after parasitization, and effects on European corn perforator larvae Treatment of leaf damage and recovery of larvae of heavy larvae (mg) Water extensive 55 / 120 0.42mg control medium widely 40/120 0 · 50mg broth (1.0% ν / ν) slight 3/120 0. 15mg printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (A read the precautions on the back before filling (This page) The broth's activity against Nicotiana tabacum A (Good eJiotAis virescens) was demonstrated using a leaf soaking method. Fresh cotton leaves were cut from the plants, and the leaves were cut with a 18.5 mm cork drill. The leaves were immersed in the control group culture solution (PP3) or the Amicon, Proflux M12 tangent filtration system with a 10kDa filter, which concentrated about 10 times the culture broth of Bacillus spp. (W-14 strain). Excess fluid is removed and the center of the leaf is clamped with straight paper clips. The paper clip was then squeezed into a 0-ounce plastic shallow cup containing about 2.0 ml of 1% agar. This line suspends the leaves on the agar. After the leaf was dried, a newborn tobacco budworm larva was placed on the leaf and the cup was capped. The cups were then sealed in a plastic bag and placed in a dark, 27GC incubator for 5 days. Larvae and leaves were weighed at this time to measure leaf damage. Table 9 Effect of Illumination on Bacillus luminescens (W-14 strain) culture broth on a cotton leaf soaked in a cotton leaf soak analysis of the final Bacillus spp. (Mg) treatment of leaf larvae-50-This paper size applies to Chinese national standards ( CNS) A4 specification (210X297 mm) 509722 A7 B7 Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs, printed and invented (48) Control leaf control culture medium bacilliform broth 3 9 4 ··· 11 ΟΛ- 11 土 土Soil 7 0 3 5 · 4 · 4. 5 3 5 na 4 · 3 ± 0 · 91 0. 〇 ** * No applicability, u No live larva was found. Example 5, Characterization of the toxin peptide composition of part A In the following analysis, the subunit of the toxin protein isolated from the bands in Example 1 was coated on a 7 ° / 〇SDS polyacrylamide electrophoresis gel with a ratio of 30: 0.8 (acrylamide: BIS-acrylamide) Parsing. This gel matrix improves the better resolution of larger proteins. The gel system used to estimate the molecular weights of the first and second subunits in Example 1 is an 18% gel with a ratio of 38: 0. 18 (acrylamide: BIS-acrylamide). Separation over a wider range of molecular sizes, but lower resolution for higher molecular weight components. In this analysis, 10 rather than 8 protein bands were resolved. Table 10 lists the molecular weights of 10 calculated analytical bands, and directly compares these molecular weights estimated under these conditions with those of the previous examples. It was not surprising to detect additional bands under the different separation conditions used in this example. Under different analytical conditions, differences between previous and new molecular weight estimates are also expected. Due to the improved resolution, higher molecular weight estimates in the analysis of this experiment were considered to be more quasi-cancer than in Example 1. However, this is an estimate based on SDS-PAGE analysis, which is usually not analytically accurate and is not used for estimates of peptides whose peptides have been further changed due to translation or modifications that accompany translation. The amino acid sequence of the N-terminal portion of 5 of the 10 resolved peptides has been determined. Table 10+ correlates the molecular weight of these proteins with the identified sequences. In the serial identification number 2, some analysts believe that the 5th-51- (please read the precautions on the t-face before filling out this page) This paper size applies the Chinese National Standard (CNS) A4 specification (210X297) (Centi) 509722 A7 B7 V. Description of the invention (49) " ^ The proline residue of the residue should be aspartic acid H amine (asn in the sequence_identification No. 3, some analyses believe that the 13th and 14th positions The amino acid residues are all arginine (airg). In the sequence identification number 4, the amino acid residue at the eighth and sixth position should be alanine (akf or 7 silk: acid (ser). In the sequence identification number 5 'Some analyses believe that the amino acid residue at position 3 should be aspartic acid (asp). Table 10 Value tt Estimated wood value ft Estimated new 201132 at 0.5 .8.5.83.Base 076652 J 2 1 value tt 6 8 2 1 estimate 8 4 · · · · ί 085065 towards 2 16 6 5 2 *

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PI DS S ο Tiger tu tu tu § ΜΊ Yun Yun Yun Yun f5 歹 IX oxw 0〇4 * ro 1i In order to cut off k, k, k, k, k, k, k, k, k, k, k, k, k, k, k, k, k, k, k, k, k, k, k, k, k, k, k, k, k, k. Symptoms: Poisonous, Fine, Undivided, Partial, Partial, Part B, Analysis, Examples, and Reality (Please read the precautions on the back before filling out this page) k pack ·-order New N-terminal sequence (ie, sequence identification number 15), AlaGlnAspGly

Asn Gin Asp Thr Phe Phe Ser Gly Asn Thr, as described in Example 5, by the consumer cooperation of the former Central Bureau of quasi-Ministry of Economic Affairs of the Ministry of Economic Affairs, described in Example 5, Part B was isolated from the natural HPLC-purified toxin for further n-terminal sequencing . This peptide is derived from the icaj gene. The peptide is labeled TcaAu, starting at position 254 and starting at position 491 from TcaAUi (sequence identification number 4). Based on the gene sequence, the peptide size was estimated to be 25,240 Da 〇fAL · Qin Susheng. The characteristics of the composition were conferred in another analysis. The toxin protein complex uses a 10% -80 ° / 〇sulfur (CNS) μ specification. 509722 A7 B7 V. Description of the invention (50) Ammonium acid precipitation and one of the subsequent ion exchange chromatography steps (Mono Q) and two molecular size determination chromatography steps are re-isolated from the growth medium of Bacillus radians. These conditions are similar to those used in Example 1. In the first molecular determination step, a spike with a biological activity was found once at 100 ± 10 kDa. Based on protein measurements, this fraction is 20-50 times less active than the large (or predominant) active spike of approximately 860 ± 100 kDa (natural). In this separation experiment, a small activity spike of approximately 325 to 50 kDa, while retaining a significant proportion of significant biological activity, was also resolved. The 352kDa spike is believed to be related to or originated from the 860kDa spike. A 56 kDa protein was resolved in this analysis. The N-terminal sequence of this protein is represented by sequence identification number 6. It is worth mentioning that this protein has significant identity and conservation at the N-terminus and sequence identification number 5. It is believed that the two can be encoded by different members of a gene family, and each gene The proteins produced are quite similar to those operable in the insecticidal toxin complex. Printed by the Consumer Cooperatives of the Central Bureau of Standards of the Ministry of Economic Affairs (read the precautions on t * before filling out this page) The 185kDa protein, which is a major and significant one, continues to appear in an amount equivalent to the third protein in Table 10. May be the same protein or protein fragment. The N-terminal sequence of this 185kDa protein is shown in Sequence ID No. 7. Another N-terminal amino acid sequence was also obtained from the isolated protein. The determined N-terminal sequence does not appear to be identical to any of the proteins in Table 10. Other proteins are present in the isolated preparation. This protein has an estimated molecular weight of 108 kDa, and its N-terminal sequence is shown as sequence identification number 8. This protein has an estimated molecular weight of 80kDa, and its N-terminal sequence is _-53-. This paper size applies the Chinese National Standard (CNS) A4 specification (210X297 mm). 509722 Printed by A7, Consumers Cooperative of the Central Standards Bureau, Ministry of Economic Affairs B7 V. Description of the invention (51) The sequence identification number No. 9 is displayed. When the protein material in the active peak of about 325 kDa was analyzed for its size, it showed a band of about 51, 31, 28, and 22 kDa. As in the case where the molecular weight is determined by the analysis of electrophoretic kinematics, these molecular weights are subject to errors due to differences in ionic strength and electrophoretic strength. One of the known techniques is that a well-defined molecular weight value cannot be determined using these standard methods and causes ambiguity. It is assumed that proteins of this size are degradation products of larger protein species (about 200 kDa in size) present in larger major toxin complexes. Finally, many preparations contain a protein having an N-terminal sequence represented by sequence identification number 10. This sequence is highly homologous to a known chaperon in protein (known to function in a large protein complex combination and a helper protein). Although the applicant is unable to consider the function of this combination as the function of the protein identified by sequence identification number 10, it is consistent with the existence of the toxin-protein complex (this concomitant protein protein may involve a combination thereof). . Furthermore, although these proteins are not directly considered to have toxic activity, this protein may be important in determining the overall structural properties of the protein toxin, and therefore may cause toxic activity or persistence of the complex in vivo after oral administration. This protein toxin complex was then analyzed for stability against proteinase K. After incubating the complex for 24 hours in the presence of proteinase K more than 10 times the number of moles, the activity almost disappeared (oral mortality decreased to about 5%). These data confirm the protein properties of the toxin. This toxic activity was also retained by a dialysis membrane, which confirmed the day again. -54 · This paper size is applicable to the Chinese National Standard (CNS) A4 specification (210X297 mm) (Please read the caution on the back J. ▼ item before Fill. Pack-: Write this page) Order 4 509722 Printed by A7 B7, Consumer Cooperatives of the Central Standards Bureau, Ministry of Economic Affairs 5. Description of Invention (52) Of course, the toxin complex is large. Green Example 7 The bacteriotoxicity of the bacillus versicolor, and the partial amino acid sequence of Lao Xunzi, and the N-methylamine sulfamidine, in a group of experiments similar to those in Examples 5 and 6, were performed. Protein ammonium sulfate precipitation is slowly added by crystallization of ammonium sulfate to adjust the bacilliform broth (usually 2-3 liters) to a final concentration of 10% or 20%. After stirring at 40 ° C for 1 hour, the material was centrifuged at 12,000 xg for 30 minutes. The supernatant was adjusted to 80% ammonium sulfate, stirred at 4GC for 1 hour, and centrifuged at 12, OOOxg for 60 minutes. The precipitate was resuspended in 1/10 volume of 100 mM Na2P04, ρΗ7.0, and dialyzed against 4GC with the same sulfate buffer. The dialyzed material was centrifuged at 12,000 xg for 1 hour before ion exchange chromatography. -An HR16 / 50 Q Sepharose (Pharmacia) anion exchange column was equilibrated with 10 mM Na2P04, ρΗ7.0. After centrifugation, the sulphuric acid precipitates after dialysis were added to the Q Sepharose column at a rate of 1.5 ml / min, and rinsed with a balance buffer of 3.0 ml / min until the optical density (0 · D · 280) was less than 0. · 100. Next, a 60-minute NaCl gradient ranging from 0 to 0.5M was added to the column at 3 ml / min, or a series of stages was washed using 0.1 M, 0.4 M, and finally 1.0 M NaCl for 60 minutes. Add to the column. The separated fractions were collected and centrifuged using Centriprep 100. Alternatively, the 'protein can be eluted by washing with a single 0.4 M NaCl without prior elution with 0.4 M NaCl. The 2m 1 part of the concentrated Q Sepharose sample was added at 0.5m 1 / miη to an HR 16 / 5〇Suprose 55 balanced with 10mM Na2P04, ρΗ7.0. This paper size applies the Chinese National Standard (CNs) a4 specifications (210X297 mm) (Please read the precautions on the back before filling out this page) k Pack ·, ιτ Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 509722 A7 B7 V. Description of the invention (53) 12 (Pharmacia) gel filtration Tubing. The column was washed with the same buffer at 5 ml / min for 240 minutes and a sample was collected for 2 minutes. The void volume material was collected and centrifuged using a Centriprep. A 2 ml portion of the concentrated Sepharosel 2 sample was added at a rate of 0.5 ml / min to an HR 16/50 Sepharose 4B-CL (Pharmacia) gel filtration column equilibrated with 10 mM Na2P04, pH 7.0. The column was washed with the same buffer at 0.5 ml / min for 240 minutes and a sample was collected for 2 minutes. The excluded protein spikes were separated a second time by adding to a gel filtration column using Sepharose CL-4B resin (proteins ranging from about 30 kDa to 1000 kDa). This fraction was resolved into 2 spikes; a minor spike (> 1000 kDa) in the empty volume and a major spike (eluted at a significant molecular weight of about 860 kDa). During the 1 week period, the subsequent gel filtration sample gradually showed a third peak (about 325 kDa), which appeared to originate from the main peak, perhaps due to limited protein breakdown. Biological analysis of the third spike showed that the void peak was inactive and the 860kDa toxin complex isolated part was highly active. The 325kDa peak was quite effective but had low activity. SDS-PAGE analysis of Sepharose CL-4B toxin complex from different fermentation products showed two different peptide maps' indicated by "p" and "S". In this two-part spectrum, the concentration of the peptide composition and the molecular weight are significantly different in the separated parts. For the longest time, the "S" pattern contains 4 high molecular weight peptides and the "p" pattern contains 3 high molecular weight peptides. In addition, the "S" peptide isolated portion was found to be resistant to European corn perforator 2- 3 times the activity. This change may be related to the generation number of inoculation and / or other factors based on long-term growth parameters. _____ ^ _ This paper size applies the Chinese National Standard (CNS) A4 specification (210X297 mm) ---- ----- 0 ^-(I read the t-face precautions before filling out this page), tr d printed by the Consumer Cooperatives of the Central Bureau of Standards of the Ministry of Economic Affairs 509722 A7 B7___ V. Description of the invention (54) Milligram amount The spiked toxin complex separated from the peptide map identified as "P" or "S" was prepared by SDS-PAGE and transferred to a PVDF membrane (ProBlottTM, Applied Biosystems) with TRIS-glycine (SeprabuffTM) for 3-4 Hours. The crushed material was sent to Harvard MicroChem and Cambridge ProChem for amino acid analysis and N-terminal amino acid sequencing. The three winning versions in the "S" map are compared with the sequences identified in the previous examples, which are unique. N-terminal amino acid sequence. One 2 The 01kDa (TcdAH) peptide is hereinafter referred to as sequence identification number 13 and has 33% amino acid identity and 50% similarity to sequence identification number 1 (TcbAii) (similarity and identity are borrowed from Calculate). (The vertical line in Table 10 indicates that the amino acid is the same, and the colon indicates the retained amino acid substitute.) A 197kDa secondary peptide (sequence identification number 14, TcdB), which is the same as the sequence identification number No. 2 (TcaC) has 42% amino acid identity and 58% similarity (similarity and identity are calculated by hand). A third peptide of 205kDa is represented by "TcdA". In addition, a winning version of at least 235 kDa with a limited N-terminal amino acid sequence (sequence identification number 16, TcbA) is similar to the amino acid sequence (sequence identification number 12) derived from a selected gene. And it contains the deduced amino acid sequence corresponding to the sequence identification number 1 (TcbAu) (sequence identification number η). This shows that the larger 235+ kDa peptide is proteolytically processed into the 201 kDa peptide (TcbAu, sequence identification number 1) during fermentation, which may lead to activation of the molecule. In another sequence, the sequence originally published as Sequence Identification Number 5 (1 ^ & 11) in the previous Example 5 was found to contain a day aspartate residue (Asp) instead of glycine at position 3 Acid (Gly) and amino acids Gly and Asp at positions 8 and 9, respectively. In the other two sequences ____ 二 57- This paper size is applicable to the Chinese National Standard (CNS) A4 specification (210X297 male H ~ --- -------- ^ equipment-(t first read the f side Please pay attention to fill in this purchase) Order d 509722 A7 B7_ __ V. Description of the invention (55) column (sequence identification number 2 [TcaC] and sequence identification number 3 [TcaBi]) to obtain additional amino acid sequences Density quantification was performed using a sample identical to the "S" preparation submitted for N-terminal analysis. This analysis shows that the 201 kDa and 197 kDa peptides represent the total bitterness in the "S" profile, respectively. 7.0% and 7.2% of sapphire blue-stained proteins, which are similar in content to other superior peptides. These peptides are speculated to represent protein homogeneity, similar to other bacterial toxins (such as different Cry I Bt toxins). These proteins have similarities ranging from 40-90% in their N-terminal amino acid sequences (which constitute a toxic fragment). Internal amino acid sequencing Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (t (Read the precautions on the r side before filling out this page.) To promote the selection of toxin peptide genes, select The internal amino acid sequence of the peptide was obtained as follows. A milligram amount of SDS-PAGE prepared from the 2A spike separation portion of the peptide pattern identified as "P" or "s" was converted to TRIS-glycine (SeprabuffTM) PVDF membrane (ProBlottTM, Applied Biosystems), which lasted for 3 to 4 hours. The disrupted material was sent to Harvard MicroChem and Cambridge ProChem for amino acid analysis and N-terminal amino acid sequencing. They were called TcbAH (including sequence identification number) No. 1), TcdAn and TcaBi (including sequence identification number No. 3) three peptides were trypsinized by Harvard MicrocroChem, and HPLC chromatography was used to isolate individual peptides. Selected trypsinization The peptide fragments were analyzed for N-terminal amino acids. The internal peptides of the two TcdAu peptides (205 kDa peptides) were sequenced and called TcdAH-PTlll (sequence identification number 17) and Τε (1ΑΗ- PT79 (sequence identification number 18). The two kinds of TcaBi peptides (68 kDa peptides) are sequenced and called TcaBi-PT158 (sequence identification code ____ ^ _ This paper standard applies to Chinese national standards (CNS) A4 size (210X297mm) Central Standard of Ministry of Economic Affairs 509722 A7 B7 printed by quasi-station employees' cooperatives V. Invention Description (56) No. 19) and TcaBi_PT108 (Serial Identification Number 20). Within the four TcbAu wins (201 kDa peptides), the peptides were determined Sequence, called TcbAn-PT103 (sequence identification number 21), TcbAu-PT56 (sequence identification number 22), TcbAn-PTSKa) (sequence identification number 23) and TcbAH-PT81 (b) (sequence Identification number 24). Table 11 N-terminal amino acid sequence (similarity and identity by hand calculation) 201 kDa (33% identity and 50% similarity to sequence identification number 1) LIGYNNQFSG * A Sequence identification number 13

: II I: I fiqgysdlfgn-a sequence identification number 1 197 kDa (42% identity and 58% similarity to sequence identification number 2)

MQNSQTFSVGEL sequence identification number No. 14 11: 1 I :: IMQDS Ρ Ε VSITTL sequence identification number No. 2 Exemplary case 8 Condensing body of D. luminifera W-14 bacillus gene DM Chi Min • Construction of the library and its Screening to isolate a peptide encoding the peptides constituting the rolled-up white matter toiletry. Prerequisites for the production of Toxococcus elegans insect toxic proteins and other uses in heterogeneous hosts require the isolation and assignment of those encoding their peptides. Genetic characteristics. This goal is done simultaneously. One of the methods described later is based on the use of single-source and multi-source antibodies against the purified toxin, which was subsequently used to isolate strains from the expressive gene bank. Another method described in this example is based on the use of N-terminal and internal amino acid sequence data to design degenerate oligonucleotides for PCR amplification. Either method is used to identify DNA strains containing peptide-encoding genes to isolate individual DNA base sequences. Tuya -----------------59 _ This paper is suitable for the national standard (CNS) A4 size (21GX & 7mm) of the country of wealth ------ (please first (Please read the notes on the back and fill in this page.) -Installed ·, ιτ 509722 A7 B7 Printed by the Consumers' Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs. 5. Description of the invention (57) Isolation of DNA from light-emitting Bacillus sp. W-14 strain ( ATCC Accession No. 55397) was grown on 2% No. 3 protein glycoprotein gland (PP3, Difco Laboratories, Detroit, MI). The insecticidal toxin ability was inherited by subsequent biological analysis using the method described in Example 1. maintain. A 50 ml shake culture was generated in a 175 ml throttling flask containing 2% of the No. 3 proteoglycan peptone medium at 280 C at 150 rpm for about 24 hours. 15 ml of this culture was pelleted by centrifugation and frozen at -20% in its medium until it was thawed for DNA isolation. The thawed culture was centrifuged (700 xg, 30 minutes) and the floating orange mucopolysaccharide material was removed. The remaining cellular material was centrifuged (25,000 x g, 15 minutes) to pellet the bacterial cells, the medium was removed and discarded. Genomic DNA was isolated by modification of the CTAB method described in Current Protocols in Molecular Biology (Ausubal ei a / · Eds, John Wiley & Sons, Section 2.1. [The modification includes a salt Shock and increase all volumes by a factor of 10]. The pelleted cells were resuspended in TE buffer (10mM Tris-HCl, ImMEDTA, ρΗ8 · 0) to a final volume of 10ml, and 12ml of 5M NaCl was added; Centrifuge at 2000xg for 20 minutes. The pellet was resuspended in 5.7 ml TE buffer, and 300 ml of 10% SDS and 60 ml of 20 mg / ml proteinase K (Gibso BRL Products, Grand Island, NY; in sterile distilled water) were added. In this suspension, this mixture lasted for 1 hour at 37QC; about 10 mg of lysosome (Worthington Biochemical Corp., Freehold, NJ) was added. After 45 minutes, 1 ml of 5M NaCl and 800 ml of CTAB / NaCl solution ( 10% w / v CTAB, 0.7M NaCl). This preparation is cultured at 65 ° C-60 ° (Please read the precautions on the back before filling this page) 4-Refill the item · Packing · Binding paper size applies China National Standard (CNS) A4 specification (210X297 mm Printed by the Consumers' Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs 509722 A7 _______B7_ V. Description of the Invention (58) The incubation lasts for 10 minutes, then gently shakes and incubates again, and shakes for about 20 minutes to help clarify the cell material. Add an equal volume of gas Imitation / isoamyl alcohol solution (24: 1, v / v), gently mix and centrifuge. 2 times with equal volume of PCI (phenol / chloroform / isoamyl alcohol; 50: 49: 1, v / v / v; lMTris-HC1, ρΗ8 · 0 equilibration; after extraction by Intermountain Scientific Corporation, Kaysvilie (UT), the DNA was precipitated with 0.6 times the volume of isoamyl alcohol. The DNA sink was gently removed with a glass rod and 70% The strips were washed twice with ethanol and dried, and dissolved in 2 ml of STE (10 mM Tris-HCl, pH 8.0, 10 mM NaCl, 1 mM EDTA). This preparation contained 2.5 mg / ml DNA, which was borrowed at an optical density of 260 nm ( That is, OD26Q) was measured. The molecular weight range of the isolated genomic DNA was evaluated for the applicability of gene bank construction. CHEF gel analysis was performed on a BioRad CHEF-DR 11 device with a Pulsewave 760 exchanger (Bio-Rad Laboratories, Inc., Richmond, CA) in 0.5X TBE buffer (44.5 mM Tris-HCl, ρΗ8 0, 44.5mMH3B03, 111111 £ 0 Ding) in 1.5% adipose sugar (86 & 1 ^ 111®! ^, FMC BioProducts, Rockland, ME). The execution parameters are: the initial A time is 3 seconds; the final A time is 12 seconds; 200 volts; the execution temperature is 4-18QC; the execution time is 16. 5 hours. Ethidium bromide staining and gelation under UV light showed that the size of the DNA was between 30-250 kbp. Construction of gene bank This DNA preparation of Bacillus genus was partially digested. This method is based on Ausube 1 (previously) section 3.1.3. Modifications include performing smaller-scale reactions under different conditions until near-appropriate results are achieved. Perform many scale-up reactions under different conditions and combine these results _ _-61- This paper size applies to China National Preparation Standard (CNS) Α4 specifications (210, / 297 male thin 7 (Please read the notes on the back before filling (This page) 1 pack, 1T 509722 A7 B7 V. Description of the invention (59) Analysis on CHEF gel, only the best large-scale preparation will continue. In a suitable example, the 200 μδ bacillus genomic DNA is in 2ml total volume of IX NEB 4 buffer (provided by the manufacturer as ι〇χ) and 1.5 units of 5 ^ B / (New England Biolabs, "NEB ,,, Beverly, MA) at 37 ° C Central Standards of the Ministry of Economic Affairs Printed by the Bureau's Consumer Cooperative (A read the precautions on t * face and then fill out this page) and incubate for 15 minutes. The reaction was terminated by adding 2ml PCI and centrifuged at 8000xg for 10 minutes. 200μ1 of 5M NaCl and 6ml of ice-temperature ethanol Supernatant. This preparation was refrigerated at -20GC for 30 minutes and then centrifuged at 12,000xg for 15 minutes. This supernatant was removed and the precipitate was dried in a vacuum oven at 40oC and then resuspended in 400μ1 Within STE. Spectral analysis shows input Approximately 40% of the DNA is recovered. The digested DNA is separated in size on a sucrose gradient according to Section 5.3.2 of CPMB (previously). A 10% to 40% (w / v) linear sucrose gradient is divided by- The gradient maker was prepared in an Ultra-ClearTM tube (Beckman Instruments, Inc., Palo Alto, CA), and the MA sample was overlaid. After centrifugation (26, OOO rpm, 17 hours, Beckman SW41 turntable, 20QC), the separation portion (about 750μ1) was extracted from the top of the gradient and analyzed by CHEF gel electrophoresis (previously). The separation part containing Aί / fragment / fragment with a size ranging from 20-40kbp was selected, and MA was borrowed from Ausubel (previously) in Section 5.3.3 Modification of the method (the amount of all solutions increased by about 3 times) and precipitated. After overnight precipitation, the DNA was collected by centrifugation (17,000xg, 15 minutes), dried, redissolved in TE (to a total volume of 80 μ1) and 8 μ1 of 3M sodium acetate and 220 μ1 of ethanol were added to reprecipitate. The precipitate collected by centrifugation as above was resuspended in 12 μ1 TE. The concentration of the DNA was borrowed from a Hoefer TK0100 fluorometer (Hoefer Scientific Instruments, San Francisco, CA Hoechst 33258 According to the size ___ -62 · _ This paper size is applicable to the Chinese National Standard (CNS) A4 specification (210 > < 297 mm) Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 509722 A7 __________B7 _ V. Invention Note (60) Approximately 2.5 pg from the DNA was recovered. 3 (^ g condensate pWE15 DNA (Stratagene, La Jolla, CA) completes its digestion with 100 units of BamHl restriction enzyme in the manufacturer's buffer (final concentration of 200 μl, 37 GC, 1 hour). ΙΟΟμΙ PCI extraction, the DNA was precipitated from the aqueous phase by adding 20μ1 of 3M sodium acetate and 550μ1 of -20G absolute ethanol. After 20 minutes at -70 ° C, the DNA was centrifuged (17, OOOxg, 15 minutes). Collect, dry in vacuum, and dissolve in 180μ1 of 10mM Tris-HCL, ρΗ8 · 0. Add 20μ1 of 10X CIP buffer (100mM Tris-HC1, ρΗ8 · 3; 10mM ZnCl2; 10mM MgCl2) and 1μΐ (0.25 units) ) 1: 4 dilution of calf intestinal alkaline phosphatase (Boehringer Mannheim Corporation, Indianapolis, IN). After 30 minutes at 37 GC, add the following: 2 μΐ 0.5 M EDTA, pH 8. 0; 10 μ1 10% SDS; 0.5 μl of 20 mg / ml proteinase K (as before), followed by incubation at 55 GC for 30 minutes. After continuous extraction with 100 μΙΡΠ and 100μΙ phenol (Intermountain Scientific Corporation, 1M Tris-HC1, ρΗ8 · 0 equilibration), the removal was performed. Phosphorylated DNA by adding 72μ1 of 7.5 M ammonium acetate and 550μ1 of -20QC ethanol were incubated on ice for 30 minutes and then centrifuged as above to be precipitated. The precipitated DNA was washed once with 500μ1 of -20oC ethanol, dried in vacuum, and then dissolved in 20μ1 TE buffer The size / fragment-based separation / fragmentation with the pWE15 vector digested and acidified was performed using T4 ligase, with the modification of the experimental procedure in Section 33 of Ausubel (using l0 provided by the premix manufacturer). x connection buffer). The connection was performed overnight at 15GC in a total volume of 20μ1, and then stored at -20oC. __- 63- This paper size applies the Chinese National Standard (CNS) A4 specification (210X297 mm) I- ------- 0 ^ ------ 1T ------ 0-* (Please read the precautions on the back before filling out this page) Printed by the Employees' Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs 509722 A7 B7 V. Description of the invention (61) 4μ1 condensate DNA ligation reaction product (containing about 1μ2 DNA) was wrapped into lambda phage using a commercial packaging extract (Gigapack® III Gold Packaging Extract, Stratagene) according to the manufacturer's instructions Inside. The wrapped preparation was stored in 4GC before use. The encapsulated agglomerate preparation was used to infect E. coli XL1 Blue MR cells (Stratagene) according to the Gigapack® 111 Gold experimental procedure ("Titrate the agglomerate gene bank") as follows. XL1 Blue MR cells were grown at 37GC in LB medium containing 0.2% (w / v) maltose plus 10 mM MgS04 (g / L: Bacto-tryptone, 10; Bacto-yeast extract, 5; Bacto-agar, 15; NaCl, 5; [Difco Laboratories, Detroit, MI]). After 5 hours of growth, the cells were sunk at 700 xg (15 minutes) and resuspended in 6 ml of 10 mM MgS04. This culture density was adjusted to OD6QG = 0.5 with 10 mM MgS04. The encapsulated condensate gene bank was diluted 1:10 or 1:20, 25μ1 in sterile SM medium (0 · 1M NaCl, 10mM MgS04, 50mM Tris-HCl, ρΗ7.5, 0.01% w / v gelatin). The diluted preparation was mixed with 25 μ1 of diluted XL1 Blue MR cells. The mixture was incubated at 25GC for 30 minutes (without shaking), then 200 μl of LB broth was added, and the incubation was continued for about 1 hour with gentle shaking from time to time. A portion (20-40 µ1) of this culture was spread on LB-Amp1G () containing 100 mg / l of ampicillin and incubated overnight at 37GC. In order to preserve the gene library without amplification, pick a single colony and inoculate it into individual wells of a sterile 96-well microwell plate; each well contains 75 μ1 Terrific Broth (TB medium: 12g / l Bacto-tryptone, 24g / l Bacto-yeast extract, 0.4% v / v glycerol, 17mM KH2P04, 72mM K2HP04) plus 100mg / l ampicillin (also known as TB-Amp1Q ()), cultivated overnight at 37GC-64- This paper scale applies Chinese National Standard (CNS) A4 Specifications (210X297 mm) I -------- ^ ------ IT ------- (t read the precautions on the back before filling in this page) 509722 A7 B7 V. Description of the invention (62) (no shock). After copying the 96-well plate to a duplicate plate, 75 μl / well of filter-sterilized TB: glycerol (1: 1, ν / ν; with or without 100 mg / l ampicillin) was added to the disk. The 370C was briefly oscillated at 100 rpm, followed by

Parafilm® (American National Can, Greenwish, CT) was sealed and stored in a -70 ° C refrigerator. The replica disk is grown and processed in the same way as the master disk. A total of 40 such master disks (with their duplicate disks) were prepared. Screened with radioactively labeled stinger needles. Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (I read the precautions on the back before filling out this page). To prepare a colony filter for detection of radioactively labeled probes, Ten 96-well plates were thawed at 25 ° C (desktop at room temperature). A replication tool with 96 prongs was used to inoculate a fresh 96-well replication disk containing 75 μl / well of TB-Amp1Q (). The replicated discs were grown (still) at 37GC overnight, followed by shaking at 37 rpm at 37 rpm for about 30 minutes. Since some isolates were not growing, these replicated dishes represented a total of 800 colonies. This replication tool was used to inoculate a replicated version of the 96-well analysis to 11 & Bo 118 (1 ^ 1,? 631; 1) 〇1'〇, ΜΑ) Cailong membrane (0.45 micron, 220x250mm), which has been It was placed on a solid LB-Amp1Q () (100ml / plate) in a Bio-assay plastic plate (Nunc, 243x243x18mm; Curtin Mathison Scientific, Inc., Wood dale, IL). The colonies were grown on the membrane at 37GC for about 3 hours. 0 A positive control colony (a colony containing a GZ4 sequence insert, see below) was grown on LB medium (ie LB- Cam35) is a separate Magna NT membrane (Nunc, 0.45 micron, 82mm round), which is treated with the gene bank colony membrane. The colonies on these membranes were lysed, and the DNA was in accordance with GeniusTM System User Guide Version 2.0 (Boehringer -65- This paper size applies to the Chinese National Standard (CNS) A4 specification (210X297 mm) 509722 A7 B7 V. Description of the invention (63)

One of the experimental procedures of Mannheim, Indianapolis, IN) was denatured and neutralized. Place the membrane with the colony facing up, place it on filter paper dipped in 0.5NNaOH and 1.5M NaCl for 15 minutes to denature, and filter paper dipped in 1M Tris-HC1, pH 8. 0, 1.5 M NaCl for 15 minutes And it. After crosslinking with a Stratagene UV Stratalinker set up in an automatic horizontal connection, the membranes were dried and stored at 25GC for use. The membrane was trimmed into strips containing a replicated version of a single 96-well plate, followed by extensive washing by CPMB section 6.4: 1 at 25 ° C in 3XSSC, 0.1% (w / v) SDS Prepared for the hybridization step for 3 hours, followed by hours at 650C in the same solution, followed by dipping in 2XSSC. (20XSSC = 3M NaCl, 0.3M sodium citrate, ρΗ7.0). Gene specific gene body fragment amplification is based on the N-terminal amino acid sequence (herein the sequence identification number 2) determined for the purified TcaC peptide isolated portion, a pool of degenerate oligonucleotides (S4Psh group) Applying standard β-cyanoethyl chemistry, applied

BioSystem ABI394 DNA / RNA Synthesizer (Perkin Elmer,

Foster City, CA). These nucleotides are printed at 55QC to protect the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (if you read the notes on the back and then fill out this page) (deprotect) 8 hours, dissolved in water, quantified by spectrometer measurement, Dilution standby. This group corresponds to the N-terminal amino acid sequence of the TcaC peptide determined. The determined amino acid sequence and the corresponding degenerate DNA sequence are as follows, where A, C, G, and T are standard DNA bases, and I represents inosine: Met Gin Asp S.er Pro Glu Val S4Psh 5'ATG CA (A / G) GA (T / C) (T / A) (C / G) (T / A) CCI GA (A / G) GT 3, another set of degenerate nucleotides was Synthetic (P2 · 3.5R group), its representative sequence ___- 66- This paper size is applicable to China National Standard (CNS) A4 specification (210X297 mm) 509722 Printed by A7 B7 of the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs Description of the Invention (64) The complement of the coding unit of the amino acid sequence determined by the identification number 17: Ala Phe Asn lie Asp Asp Codon 5'GCN TT (T / C) AA (T / C) AT (A / T / C) GA (T / C) GA (IVC) 3 'P2.3.5R 3, CG (A / C / G / T) AA (A / G) TT (A / G) TA ( T / A / G) CT (A / G) CT (A / G) 3 These oligonucleotides are used as primers in polymerase chain reaction (PCR®, Roche Molecular Systems, Branchburg, NJ) to prepare from A specific MA fragment was amplified from the genomic DNA of the Corynebacterium W-14 strain (see above). A typical reaction (50μ1) contains P2Psh and P2. 3 5R primer groups each 125 pmol and genomic template DNA 253 ng, dATP, dCTP, dGTP and dTTP lOnmol each, IX GeneAmp® PCR buffer and 2.5 units of AmpliTaq® DNA polymerase ( Both are from Roche Molecular Systems; 10X GeneAmp® buffer is 100 mM Tris-HCl, ρ 8.3, 500 mM KC1, 0.01% w / v gelatin). The amplification system used 35 cycles of 94 ° C (1.0 minutes), 55GC (1.0 minutes), 72GC (3.0 minutes), and one subsequent extension period of 72oC (7.0 minutes) in a Perkin Elmer Cetus DNA Thermal Cycler. (Perkin Elmer, Foster City, CA) Performed 0 amplification products by electrophoresis through 2% w / v NuSieve® 3: 1 agarose (FMC Bioproducts in TEA buffer (40mM Tris-acetate, 2mM EDTA, ρΗ8 · 0)) ) Analyze it. By staining the gel with ethidium bromide (5 pg / mi) and viewing under ultraviolet light, a specific product with an estimated size of 250 bp appeared in many amplification products. An area of the gel containing about 250 bp of product was excised, and a small plug (0.5 mm in diameter) was removed to provide a template for PCR amplification (40 cycles). _ _67_ This paper size is in accordance with Chinese National Standard (CNS) A4 specification (2ΗΓ × 297 公 嫠 Ί " (Please read the notes on the back _ 4 • Items are refilled and installed-write this page) Order 509722 A7 __B7 V. Invention Explanation (65) (Please read the precautions on the back before filling out this page) This reaction (50μ1) contains the same composition as above, but no genomic template MA. After amplification, 1 unit of T4DNA polymerase ( NEB), lnmol ATP, and 2.15 T4 kinase (Pharmacia Biotech Inc., Piscataway, NJ) were incubated at 25QC for 20 minutes to make the ends of these fragments blunt and phosphorylated. The DNA fragments were passed by electrophoresis 1% w / vGTG® agarose (FMC) in TEA was separated from the remaining primers. A gel slice containing a clear 250 bp fragment was excised 'The DNA was made using a Quaex kit (Qiagen Inc., Chatsworth, CA) Extracted. Printed by the Consumer Cooperatives of the Central Bureau of Standards of the Ministry of Economic Affairs.

The extracted DNA fragment was ligated to a plastid carrier PBC KS (+) (Stratagene) which had been digested with restriction enzyme 3 / complete digestion and extracted in a similar manner to the aforementioned PWE15. A typical ligation reaction (16.3 μ1) in IX ligation buffer (50 mM Tris-HC1, ρΗ7.4; 10 mM MgCl2; 10 mM dithiothreitol; ImM spermine, lmMATP, 100 mg / ml bovine serum albumin) It contains 100ng digested pBC KS (+) DNA, 70ng 250bp fragment MA, lnmol [Co (NH3) 6] C1, and T4 DNA ligase in 3.9 Weiss units. After overnight incubation at 14QC, the ligation product was transformed into frozen competent E.coli DH5oc cells (Gibco BRL) according to the supplier's recommendations and spread on IPTG (119pg / ml) and J-Fin / (50pg / ml) ) On the LB-Cam35 disc. Independent white colonies were picked and plastid DNA was prepared by a modified alkali-lysis / PEG precipitation method (PRISMTM Ready Reaction DyedeoxyTM Terminator Cycle Sequencing Kit protocols; ABI / Perkin Elmer). The nucleotide sequence of the two strands of the embedded DNA uses the T7 primer [pBC KS (+) 601-623 bases: AAACGACGGCCAGTGAGCGCG], LacZ -68- This paper is in accordance with the Chinese National Standard (CNS) A4 specification (210 X 297 public) 509722 A7 B7 V. Explanation of the invention (66) Primer [pBC KS (+) 792-816 salt-based ATGACCATGATTACGCCAAG-CGCGC] and the experimental procedure provided by the PRISMTM sequencing kit (ABI / Perkin Elmer) determine. Unincorporated dye-terminating dideoxyribonucleotides were removed by Centri-Sep 100 columns (Princeton separations, Inc., Adelphia, NJ) according to the manufacturer's instructions. The DNA sequence was obtained by analyzing the sample on an ABI Model 373A DNA Sequencer (ABI / Perkin / Elmer). The DNA sequences of the two isolates GZ4 and HB14 are shown in Figure 1. Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs · (Please read the notes on the back before filling this page) This sequence shows the following characteristics: i) The 1-20th base represents the S4Psh degenerate oligonucleotide. One of the 64 possible sequences, ii) the sequence of the 1-3 and 6-12 amino acids exactly corresponds to the N-terminus of TcaC (sequence identification number 2 herein), iii) the The four amino acids are cysteine and not serine. This difference is encoded in the degenerate serine codon (see above), iv) the fifth amino acid encoded is proline, which corresponds to TcaC known as sequence identification number 2 N-terminal sequence, v) the 257-276th base code is designed into the 192 possible sequences of the degenerate group vi) the TGA stop codon of the 268-270th base is introduced into the corresponding position The result of the complementarity (which is constructed in the oligonucleotide group) does not indicate a shorter read structure of the corresponding gene. A TcaC peptide gene specific probe is labeled corresponding to the above 276 base-based DNA fragments amplified by PCR® in a 100 μ1 reaction volume (35 cycles), which uses P2PshP and 2.3.5R primers Each lOOpmol, using 10ngGZ4 or HB14 plastids as templates, dATP, dCTP, ____ This paper size applies Chinese National Standard (CNS) A4 specification (210X 297 mm) " 509722 A7 B7 V. Description of the invention (67) dGTP and dTTP Each 20nmol, 5 units of AmpliTaq® DNA polymerase and IX concentration GeneAmp® PCR buffer were prepared at the same temperature as before. The amplified products were extracted from a 1% GTG® agarose gel using a Qiaex kit and quantified by a fluorometer. The extracted amplification product (about 400ng) from HB14 plastids was divided into 5 equal portions and labeled with 32P-dCTP using the High Prime Labeling Mix (Boehringer Mannheim) according to the manufacturer's instructions. Unincorporated radioactive isotopes were removed by a NucTrap® Probe Purification Column (Stratagene) according to the supplier's instructions. The specific activity of the labeled DNA product can be determined by scintillation counting to be 3.11 X 108 dpm / pg. The labeled DNA was used to probe the membranes of 800 members of the gene bank. Printed with a TcaC-winning version of a specific probe produced by the Consumers' Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (#. Read the precautions on page t before filling this page) Boil the radioactively labeled HB14 probe for about 10 Minutes, and then added to the "minimal hyb" solution. [The "minimum hybridization" method is taken from the CERES experimental procedure; "Restriction Fragment Length Polymorphism Laboratory Manual" 4th edition, sections 4-40 and 4-47; CERES / NPI, Salt Lake City, UT. NPI is now abolished , Its successor operates under the name Linkage Genetics]. The "minimal hyb" solution contains 10% w / v PEG (polyethylene glycol, W. about 8000), 7% w / v SDS, 0 · 6X SSC, 10 mM sodium phosphate buffer (from a 1M concentrate containing 95 g / l NaH2P04.lH20 and 84.5 g / l Na2HP04.7H20), 5 mM EDTA, and 100 mg / ml denatured salmon sperm MA. The membrane was briefly dried, Subsequent to prehybridization, 5 membranes were placed on the _-_ containing 75ml "minimum hybridization" and 2. 6ng / ml radioactive label. This paper is in accordance with China National Standard (CNS) A4 (210 X 297) (Mm) 509722 Printed by A7 ___B7 of the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 5. Description of the invention (68) 2 plastic boxes of HB14 probes. These are cultivated overnight at 60QC with slow shaking (50rpm). These Filter in 25GC "minimum hybridization wash solution (0.25X SSC, 0 · 2% SDS) "for 3 times, each time about 10 minutes, and then washed in the same solution at 60 GC for 2 times for 30 minutes with slow shaking. These filters were placed in Saran Wrap® (Dow Brands). , Indianapolis, IN) on a piece of paper with 2 Dupont Cronex Lightning-Plus Cl enhancements (enhancers) in a light-shielding automatic radiographic development cassette so that x—〇matx light film (Kodak, Rochester, NY) is in the- Exposure at 70 ° C for 4 hours. After development (standard photographic procedure), a clear signal appears in a weak and irregular high-volume background. The filters are washed at 68GC in the "minimum hybridization washing solution" for about After 4 hours, it was placed in the cassette, and the film was exposed at -70 ° C overnight. Due to strong signals on the two replicated 96-well colony replicas, 12 possible positives were identified. Negative control membrane ( The colony of XL1 Blue MR cells containing PWE15) showed no signal, and a very strong signal was seen in the positive control membrane (the DH5a cell of the GZ4 isolate containing the PCR product) processed at the same time as the experimental sample. Twelve putative hybridization-positive colonies were taken from frozen 96-well gene bank trays' and grown on solid LB-Amp1QQ medium at 370C overnight. They were then transferred to solid LB-Amp1G () medium (3 per plate, plus 3 negative controls: XL1 61116 cells containing the PWE15 vector). Two sets of membranes (from Naruto, 0.45 micron) were prepared for hybridization. In the first group, a filter membrane was directly placed on a colony in a plate, and the attached bacterial cells were removed and treated as follows. The second set of filter membranes was placed on a plate containing LB-Amp1G () medium, and then the cells on the plate were transferred to the filter plates for seeding. 37GC growth overnight _ _ -71-_ This paper size applies Chinese National Standard (CNS) A4 specification (210X297 male foot 1 ~ " --------------- 1T ---- -• * (Please read the precautions on the back before filling out this page) 509722 A7 B7 5. After the description of the invention (69), the filters are removed from the discs and processed. Bacterial cells on the filters After being lysed, the DNA was denatured by placing each filter colony upward on 0.5N NaOH (1.0 ml) in a plastic dish, which was denatured for 3 minutes. The filters were blotted on a paper towel, and then Repeat the procedure with fresh 0.5N NaOH. After blotting, the filters were neutralized by placing each piece on 1ml of 1M Tris_HCl, ρΗ7.5 for 3 minutes, blotted dry, and neutralized again with fresh buffer. Immediately immerse it in 0.5M Tris-HCl, ρΗ7.5 and 5M NaCl twice (5 minutes each time). After blotting, the DNA is cross-linked to the filter by UV (as before). The membrane was washed in approximately 100 ml of 3X SSC plus 0.1% (w / v) SDS (25GC, 100 rpm; 4 times with fresh buffer each time). It was then placed in the minimum volume before the hybridization solution. Medium [6X SSC plus Ficoll 400 (Pharmacia), polyvinylpyrrolidone (average MW 360,000; Sigma) and bovine serum albumin 5th separation (Sigma) 1% w / v each at 65GC, 50 rpm for 2 hours. Remove the pre-hybridization solution Replaced with the HB14 32P-labeled probe stored in the previous hybridization of the gene library membrane, which has been denatured at 95GC for 5 minutes. The hybridization was shaken at 60 rpm at 50 rpm for 16 hours. Remove the labeled probe After injecting the solution, the membranes were washed 3 times in 3X SSC (approximately 150ml each wash) at 25GC (50rpm, 15 minutes). They were added at 0.25X SSC and 0.2% SDS (minimum hybridization washing solution) at 68oC (50rpm). Wash for 3 hours, and then expose the X-ray film for 1.5 hours at 25GC as described above (no enhancement screen). This exposure shows strong hybridization signals on the aggregates 22G12, 25A10, 26A5 and 26B10, and the aggregate Isolate 8B10 showed a very weak signal. Negative control (pWE15) showed no sign of colony, a very strong -72- This paper size applies Chinese National Standard (CNS) A4 specification (210 X297 mm) ------ --- ^ 装-(t read first, fill in this page with notes on the back) Order d Ministry of Economic Affairs Central Bureau of Standards staff consumer cooperative printed 509722 A7 B7 V. Description of the Invention (7〇) signal found in the positive control membrane (DH5oc cells containing the GZ4 isolate of the PCR product) simultaneously with the test sample processing. 7 ^ and one of the specific genomic fragments are based on the N-terminal amino acid sequence (herein the sequence identification number 3) determined for the purified TcaBi peptide isolated portion, a group of degenerate oligo Nucleotides (P8F group) were synthesized as described for the TcaC peptide. The determined amino acid sequence and the corresponding degenerate DNA sequence are as follows, where A, C, G, and T are standard DNA bases, and I represents inosine: Leu Phe Thr Gin Thr Leu Lys Glu Ala Arg P8F 5, (C / T) TI TTT ACI CA (A / G) ACI (C / T) TI AAA GAA GCI (A / C) G 3 'Another set of degenerate oligonucleotides was synthesized (P8. 108 3R group), which represents the complement of the coding amino acid sequence (sequence identification number 20) of the peptide in TcaBi-PT108: aminoamidine

Met Tyr Tyr lie Gin Ala Gin Gin Codon ATG TA (T / C) TA (T / C) ATCT / C / A) CA (A / G) GC (A / C / G / T) CA (A / G ) CA (A / G)

P8. 108. 3R TAC AT (A / G) AT (A / G) TA (A / G / T) GT (T / C) CG (T / G / C / A) GT (T / C) GT ( T / C) Printed by the Consumer Cooperatives of the Central Bureau of Standards of the Ministry of Economic Affairs (please read the precautions on the back before filling this page) These oligonucleotides are used for the use of HotStart 50 TubesTM (Molecular Bio-Products, Inc., San Diego, CA) primers for PCR® to amplify a specific DNA fragment from genomic DNA prepared from a strain of Corynebacterium W-14 (see above). A typical reaction (50μ1) contains 25pmol each of P8F and P8.108.3R primer groups in (lower) 1X GeneAmp® PCR buffer, and 2nmol each of dATP, dCTP, dGTP, and dTTP; (top) in IX GeneAmp® PCR buffer Contains 230ng of genomic template MA, dATP, ______- 73-_ This paper size applies to China National Standard (CNS) A4 (210X297 mm) Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 509722 A7 B7 V. Description of the invention (71 ) dCTP, dGTP and dTTP each 8nmol and 2.5 units AmpliTaq® DNA polymerase. Amplification was performed using 35 cycles as described for the TcaC victory. Amplified products were analyzed by electrophoresis through 0.7% w / v SeaKem® LE Agarose (FMC) in TEA buffer. A specific product of an estimated size of 1600bp was found. Four of these reactions were collected, and the amplified DNA was extracted from a 1.0% w / v SeaKem® LE gel using the Qiaex kit described in TcaC peptides. The extracted DNA was directly used as a template (PRISMtmS sequence set) for sequence determination using the P8F and P & 108.3R primer groups. Each reaction contained approximately 100 ng of template DNA and a primer group of 25 pmol, which were processed according to standard experimental procedures described for TcaC peptides. Analysis of the sequence derived from the P8F primer extension revealed the short DNA sequence (with the encoded amino acid): GAT GCA TTG NTT GCT Asp Ala Leu (Val) Ala which corresponds to the sequence identification number 3 ( TcaBi) is part of the N-terminal peptide sequence. -TcaB; labeling of peptide-specific probes. Approximately 50ng of gel-purified TcaBi DNA fragments were labeled as 32P-dCTP as described above. Unincorporated radioisotopes were removed by a NICK Column® (Pharmacia). The specific activity of the labeled DNA product was determined to be 6 × 109 dpm / ^ g. This labeled DNA is used to detect and prepare the colony membrane from a member of the gene bank that has hybridized with the TcaC peptide-specific probe. These membranes containing 12 colonies identified by TcaC-probe gene bank screening (see above) were boiled for about 30 minutes 2 times, each time using 1 liter 0.1X _______: 74-This paper size is applicable to the country of China Standard (CNS) A4 specification (210 × 297 ^^ --------- ^ equipment ------ order ------ ^ * Μ (Please read the precautions on the back before filling in this page) 509722 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs A7 B7 V. Description of the invention (72) SSC is added to 0.1% SDS to remove the radioactive Tcac specific probe. The radioactive label is removed by a 6-hour film Check for exposure. The cleared membrane is then incubated with the TcaBi-specific probe prepared as described above. The labeled DNA is denatured by boiling for 10 minutes, and then added to the solution already in the minimum, hybridization at 60 0C. The filters were incubated for 1 hour. After passing through the hybridization isolator at this temperature, the probe solution was removed, and the filters were washed as follows (both in 3X SSC plus 0.1% SDS): 5 minutes at 25 0C 1 time, 1 hour at 60 ° C in fresh solution, 63 ° C: 1 hour in fresh solution. After borrowing After 1.5 hours of X-ray film exposure in quasi-procedure, four strongly hybridized colonies appeared. As made with TcaC specific probes, they were isolates 22G12, 25A10, 26A5, and 26B10. The same TcaBi probe solution waited The volume (about 100 ml), the minimum hybridization solution was diluted, and then used to screen these membranes containing 800 members of the gene bank. After hybridization, washing and X-ray film as described above After exposure, only 4 condensate strains 22G12, 25A10, 26A5, and 26B10 were found to hybridize strongly to the probe. Containing _ Straight. .Ma TcaC and YcaB; Separation of a Cause of Winning Version, DNA Salt It was decided that three hybrid positive aggregates in the XL1 Blue MR strain were grown overnight in 100 ml of TB-Amp! Mp at 30GC with shaking (200 rpm). After harvesting these cells by centrifugation, the aggregates DNA It was prepared using a commercially available kit (BIGprepTM, 5 Prime 3 Prime, Inc., Boulder, CO) in accordance with the manufacturer's experimental procedures. Only 1 agglomerate 26A5 was successfully separated by this procedure. When Digested with restriction enzymes; Μ KNEB) and coagulated -75- Applicable on paper size National Standard (CNS) Α4 Specification (210 X 297 mm) (Please read the precautions on the back before filling out this page) Installation,? Τ 509722 A7 B7 5. Description of the invention (73) Measured during gel electrophoresis analysis Fragments with approximate sizes of 14, 10, 8 (vector), 5, 3, 2.9, and 1.5 kbp. The second attempt to isolate condensate DNA from the same three strains (8ml culture, TB-Amp1GG, 30GC) was by using a boiling miniprep method (Boiling miniprep method; Evans G. and G. Wahl., 1987, "Cosmid vectors for genomic walking and rapid restriction mapping" in Guide to Molecular Cloning Techniques, Meth. Enzymology, Vol. 152, S. Berger and A. Kimmel, eds., Pgs. 604-610) has only 1 aggregation The plastid 25A10 was successfully separated by this procedure. When digested with the restriction enzyme valence M KNEB) and analyzed by gel electrophoresis, this aggregated body showed the same segmented pattern as previously seen aggregated body 26A5. Printed by the Consumers' Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (read the _ precautions on the back and then fill out this page). 26A5 0.15pg sample of condensate DNA was used to borrow 50ml of E. coli DH5a cells (Gibco BRL) Transformed by the supplier's experimental procedures. A single colony isolate of that strain was inoculated to 4 ml of TB-Amp1 () () and grown at 37 GC for 8 hours. Chloramphenicol was added to a final concentration of 225 pg / ml, the incubation was continued for 24 hours, and then the cells were harvested by centrifugation and frozen at -20 GC. The separation of 26A5 agglomerates is made by dissolving a small amount of precipitate with a standard alkali (Maniatis ei a 厶, p. 382), which is modified at each step by increasing all the volume to 50%, stirring or gently mixing instead of shaking. Of it. The DNA precipitate was washed in 70% ethanol, and then dissolved in TE containing 25gg / ml of ribonuclease A (Boehringer Mannheim). The identification of the hybridization / fragment derived from the GZ4 and TcaBt probes was about 0.4 pg. Condensate 25A10 (from XL1 Blue MR cells) and condensate 26A5 (from DH5oc cells expanded by amycin) of about 0.5 pg, respectively. ____- 76-_ This paper applies Chinese National Standards (CNS) A4 specification (210X297 mm) 509722 A7 B7 Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs. 5. Description of the invention (74) Digest with about 15 units of ibo and / (NEB) for 85 minutes, freeze overnight, and then heat at 65 0C. 5 Min, run on a 0.7% agarose gel (SeaKem® LE, 1XTEA, 80 volts, 90 minutes). The DNA was stained with a desertified beta bond as described above and photographed under ultraviolet light. The aggregate and digestion of the aggregates 25A10 are completely digested, but the sample of the aggregates 26A5 is only partially digested under these conditions. The agarose gel containing the DNA fragment was depurinated, denatured, and neutralized, followed by a southern turn on a Magna NT and Dragon membrane using a high-salt experiment (20X SSC), all described in Ausubel ei aA (CPMB) in Section 2.9. The transferred DNA was then UV-crosslinked to the nylon membrane as before. A TcaC-specific DNA fragment corresponding to the insert of the plastid isolate GZ4 was amplified by pcR® in a 100 mi reaction volume as previously described. The amplification products from three such reactions were collected and extracted from a 1% GTG® agarose gel using the Qiaex kit as previously described and quantified by a fluorometer. The gel-purified DNA (100 ng) was labeled as described above using High Prime Labeling Mix (Boehringer Mannheim) at 32P-dCTP to achieve a specific activity of 6.34 x 108 dpm / pg. Boil the 32P-dCTP-labeled GZ4 probe for 10 minutes, add to the "minimum hybridization" solution (lng / ml), then add the southern transmembrane containing digested condensate DNA, and gently shake at 50 rpm Incubate for 4 hours at 60 GC. The membrane is then washed 3 times at 25 GC for 5 minutes each (minimum hybridization wash solution), followed by 2 washings at 60 GC for 30 minutes each. The exposure time of the film (using the enhanced screen) lasted about 30 minutes. The GZ4 probe is strongly 5.0 kbp (appearance size) of this two aggregates (26A5 and 25A10). ___- 77- This paper size applies to China Standard (CNS) Α4 specification (210 × 297 mm) (Please read the notes on the back before filling out this page) Binding · d 509722 A7 B7 Printed by the Consumers' Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs ) / Fragment hybridization. The membrane was removed by boiling in 0.1X SSC plus 0.1% SDS for about 30 minutes to remove radioactivity. The radioactive label was checked by exposure to a film. It was subsequently mixed with (denatured) TcaBi at 60 ° C. The probe was previously used to screen the colony membrane (described above). Hybridize in the “minimum hybridization” buffer for 3.5 hours, and wash as described above. Expose the light film with 2 enhancement screens at -70 ° C for 40 minutes. For 2 aggregates, the ^ 3 probe slightly 50 / fragment hybridization, which strongly hybridizes to a fragment of about 2.9 kbp. The sample of agglomerates 26A5 (from DH5a cells) as described above is used as a DNA source, from which the band of interest is sub-selected This DNA (2.5 pg) was digested at about 3 unit price M / (NEB) in a total volume of 30 μl for 1.5 hours to provide a portion of the digest, as confirmed by gel electrophoresis. lOpg pBC KS (+) DNA (Stratagene) was digested at 20 units / in a total volume of 20 μ1 for 1.5 hours to provide a complete digest as confirmed by gel electrophoresis. This bivalent M / cut DNA preparation The material was diluted to 50μ1 with water, and equal volumes of PCI were added separately. The suspension was gently mixed, centrifuged in a microcentrifuge, and the aqueous supernatant was collected. The DNA was precipitated by 150μ1 ethanol, and the mixture was placed in -20 GC. Overnight. After centrifugation and drying, the and / digested PBC KS (+) was dissolved in ΙΟ ΟμΙ TE; The partially digested 26A5 was dissolved in 20μ1 TE ° DNA and the recovery was checked by a fluorometer. In individual reactions, about 60ng of valence M / digested pBC KS (+) DNA and about 180ng or 270ng of partially digested The DNA of aggregated body 26A5 was ligated. The linking system uses T4 ligase-78 from New England BioLabs. This paper size is applicable to the Chinese National Standard (CNS) A4 size (210X297 mm) (Please read the notes on the back J. ▼ item and fill in t-): (Write this page)

、 1T A7 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs " " " —— ____ B7 __ V. Description of the Invention (76) and buffer solution ’in a volume of 20μ1 at 15 ° C for 5 hours. This ligation mixture diluted to 100 μl with sterile TE was used to transform Lengkang competent DH5a cells according to the supplier's instructions. Different amounts (25-200μ1) of transformed cells were spread on freshly prepared solid LB-Cani35 medium containing lmMX_gal. The plate was placed at 37 ° C for 5 hours, and then refrigerated in the dark for about 3 hours to enhance the color of the insert selection. Pick white letters and drop them onto patch piates of the same composition, and incubate overnight at 37 GC. Two provoked colonies of each selected griddle were prepared as follows. After picking the white colonies on a fresh plate, press the round BMagna NT resistant dragon film onto the grid plate, pick up the films, and denature, neutralize and cross-connect the UV of the gene bank colony film as described above. . This horizontally provoked colony is being washed intensively 'which includes gentle wiping of excess cell debris with toilet paper. One group was hybridized with the GZ4 (TcaC) probe solution described earlier, and the other was based on the "minimum hybridization" experimental procedure to hybridize with the 1 ^ 1 probe solution described earlier, and then as described above. The gene bank colony membrane was washed and exposed to film. Colonies showing hybridization signals, whether only with the GZ4 probe, with both the GZ4 and TcaBi probes, or only the probes, were selected for further work. Isolate single colonies on LB-Cam35 medium containing IPTG and X-gal as before. About 35 single colonies from different isolates were picked into liquid LB-Cam35 medium and grown overnight at 37 ° C. These cells were collected by centrifugation, and the plastid DNA was isolated by dissolving the micro-precipitation according to one of the standard alkalis of Maniatisei a (p. 368). (: Milk) 8 4 specifications (210, 乂 297 mm) '(Please read the notes on the back of the 4 items, and then fill in and write II to write this page),? Τ.4 509722 A7 B7 V. Description of the invention (77) The precipitate was dissolved in TE + 25pg / ml ribonuclease A, and the DNA concentration was measured by a fluorometer. The analysis was performed by gel electrophoresis. The following isolates were selected for use. Isolate A17.2 contained only reconnected pBC KS (+) and was used as a (negative) control. Isolates D38 · 3 and C44. 1 each contained only 2.9 kbp TcaBi hybrid AM / fragments embedded with PBC KS (+). These plastids were named PDAB2000 and pDAB2001, respectively, and are shown in Figure 2. Isolates A35 .3 contains only about 5.0 kbp GZ4 hybrid Fen I fragment embedded in pBC KS (+). This plastid is named pDAB2002 (also shown in Figure 2). These isolates provide a template for DNA sequencing. Plastid PDAB20 0 0 and pDAB2001 were prepared as before using the BI GprepTM kit. Cultures (30 ml) were grown overnight in LB-Cam35 to an OD6G () of 2 and the plastids were then isolated according to the manufacturer's instructions. DNA precipitates were separately It was re-dissolved in 100μΙ TE, and the integrity of the sample was checked by M / digestion and gel electrophoresis analysis. Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs I -------- 0 batch of clothes _ 丨 · -(Please read the notes on the back before filling out this page) 4 Sequencing reactions are performed in two groups, one using PD AB2000 DNA was used as a template, and the other group used PDAB2001 DNA as a template. The reactions were performed using the dideoxy dye terminator cycle sequencing method, as described previously for the sequencing of GZ4 / HB14 DNA. The preliminary sequencing uses the foregoing The LacZ and T7 primers are used as primers, plus primers based on the TcaBi PCR amplification product (THl = ATmGACTGOCMTOGCTTOGG, TH12 = GAGAGTAT0CAGA00GCGGATGATCTG) 〇 After each sequenced output is arranged and edited, each is borrowed from Perkin Elmer Applied Biosystems Division SeqEd 675 software's judgment of the integrity of the chromatographic data has been shortened to between 250 and 350 bases. -80- This paper size applies the Chinese National Standard (CNS) A4 specification (210'〆297 mm). Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 509722 A7 B7 V. Invention Description (78). The next sequencing "step" is performed by selecting the appropriate sequence for the new primers. With a few exceptions, the primers (as synthesized above) are 24 bases in length and contain 50% G + C composition. The sequence of this method is applied to the two strands of about 2.9 kbp and the I segment. To further serve as a template for DNA sequencing, plastid DNA from PDAB2002 was prepared using the BIGprepTM kit. The sequencing reaction was performed and analyzed as described above. Initially, a T3 primer (pBS SK (+) 774-796 bases: CGCGCAATTAACCCTCACTAAAG) and a T7 primer (pBS KS (+) 621-643 base: GGCCGTAATACGACTCACTATAG) were used to lead the vector from the outside The sequence reads into the sequencing reaction of the embedded DNA. Another set of primers (GZ4F: GTATCGATTACAACGCTGTCACTTCCC; TH13: GGGAAGTGACAGCGTTGTAATCGATAC; TH14: ATGTTGGGTGCGTCGGCTAATGGACATAAC and LW1-204: GGGAAGTGACAGCGTTGTAATCGATAC) were made from the internal sequence to lead to the oligopeptide, and the internal sequence was borrowed from the oligo. The sequencing of the glycoside mediator is determined. From the data generated in the preliminary sequencing, new primer sets were designed and used to read the full length of about 5 kbp fragments. A total of 55 oligo primers were used to identify contiguous sequences of 4,832 total base pairs. When the DNA sequence of the PDA / fragment insert of PDAB2002 was combined with a part of the determined sequence of the pDAB2000 / pDAB2001 isolate, a total continuous sequence of 6005 bp was generated (herein disclosed as sequence identification number 25). When the long open reading frame is translated into the corresponding amino acid, the sequence clearly shows the TcaBi N-terminal peptide (revealed as sequence identification number 3), which is encoded by the 68-124 base The paper size immediately following a methylsulfide-8 sheet is applicable to the Chinese National Standard (CNS) A4 specification (210X297 mm) '~ ^ (Please read the precautions on the back before filling this page). Assembling and ordering 509722 Ministry of Economic Affairs A7 B7 printed by the Consumer Standards Cooperative of the Central Bureau of Standards 5. Description of the Invention (79) Amino acid residues (start of translation). There is a possible ribosome binding site upstream (51-58 bases), and the downstream encodes the TcaBi-PT158 endopeptide at 215-277 bases (herein disclosed as sequence identification number 19). Further downstream, a sequence encoding the peptide within the TcaBi-PT108 exists at the 1787-1822 base of the same reading structure (the sequence is disclosed herein as the sequence identification number 20). In the same reading structure, the 1946-1972 base also encodes the TcaBuN-terminal peptide (revealed here as the sequence identification number 5), and the reading structure is uninterrupted and continues to the 3632-3634 core. Translation stop codons for nucleotides. The lack of an in-frame stop codon between the end of the sequence encoding the TcaBi_PT108 and the beginning of the TcaBu coding region, and the lack of an identifiable ribosome binding site not far upstream of the coding region. Encoded by a single open read architecture of a 65th base pair starting with sequence identification number 25, these peptides are most likely derived from a single primary of 1189 amino acids by translational cleavage Gene product TcaB (131, 586 Daltons; disclosed here as sequence identification number 26). If the amino acid immediately before the TcaBu N-terminal peptide represents the C-terminal amino acid of TcaBi peptide, the predicted mass of TcaBn (627 amino acids) is 70,814 Daltons (at This disclosure is sequence identification number 28), which is slightly higher than the size (68 kDa) displayed by SDS-PAGE. This peptide may be encoded by continuous extension of a 1881 base pair (disclosed here as Sequence Identification Number 27). The C-terminus of natural TcaBi is considered to be closer to the C-terminus of TcaBrPT108. The molecular weight of PT108 [3,438 kDa; determined in the analysis of the N-terminal amino acid sequence of this peptide] is predicted to be 30 82 · This paper is again applicable to the Chinese National Standard (CNS) A4 specification (210 × 297 mm) ( Please read the notes on the back before filling out this page) Binding and printing Printed by the Consumers' Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs 509722 Α7 Β7 5. Description of the invention (80) Size of amino acids. Use the size of the winning version to name the C-terminus of the TcaBi coding region [located at 111 of the sequence identification number 28th and 604th position]. The size of the derivative of 1 ^ & 61 was determined to be 604 amino acids or 68,463 Dalton is more in line with the experimental findings. Translation of the TcaBii peptide coding region of 1686 base-pairs (here disclosed as sequence identification number 29) yields 562 amino acids with a predicted mass of 60,789 Daltons (revealed here) It is a protein with sequence identification number 30), which corresponds exactly to the observed 61 kDa. A possible ribosome binding site (3682-3687 bases) was found 48 bp upstream of the stop codon of the open reading architecture. A sequence encoding the N-terminus of the peptide TcaC (disclosed as sequence identification number 2) was found at the 3694-3726 base. The open reading structure from this N-terminal peptide has continued uninterrupted to the 6005th base (2361 base pairs, which is disclosed here as the 2361 base pairs before the sequence identification number 31) . A gene encoding a complete peptide of TcaC (approximately 165 kDa in size and about 1500 amino acids) is composed of about 4500 bp. Another isolate containing the selected valence / fragment of aggregates 26A5 and E20.6 was also identified for its homogeneity with the previously mentioned GZ4 and TcaBi probes. Agarose gel analysis of fcoi? / Digests of DNA from plastids (PDAB2004, Figure 2) preserved by this strain revealed embedded fragments of estimated size 2.9, 5 and kbp. DNA sequence analysis of the primers starting from the sequence design of the plastid pDAB2002 showed that the 3.3 kbp square cM / fragment of this pDAB2004 was adjacent to the 5 kbp valence M / fragment in PDAB2002. The open reading structure of the 2361 salt-based pairs found in PDAB2002 was uninterrupted to -83- • This paper size is applicable to the Chinese National Standard (CNS) Α4 specification (210 × 297 mm) (Please read the precautions on the back first (Fill in this page again) • Binding-Printed by the Consumer Standards Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs 509722 A7 B7 V. Description of the Invention (81) Another 2094 bases in PDAB2004 [Revealed here is the serial identification number 31 2362 to 4458 base pairs]. DNA sequence analysis using the parental condensate 26A5 DNA as a template confirmed the continuity of the open reading architecture. In short, the open reading architecture Ucaf sequence identification number 31) is composed of 4455 base pairs and encodes a protein of 1485 amino acids (TcaC; disclosed here as the sequence identification number 32). The calculated molecular size of the 166, 214 Dalton is the same as the estimated size of the TcaC peptide (165 kDa), and the derived amino acid sequence is the TcaC N-terminal sequence [sequence identification number 2] The revealers match exactly. The amino acid sequence corresponding to sequence identification number 17 (used to design the degenerate oligonucleotide primer group within the found sequence) was shown to be found in isolates GZ4 and HB14 (formerly known as The primers in the preliminary gene bank screening) were generated by PCR-products by the reverse-strand priming of one of the primers in the degenerate primer group. In addition, the derived protein sequence does not include the internal fragment disclosed herein as Sequence Identification Number 18. These sequences show that plastid PDAB2004 contains the complete coding region of the TcaC peptide. Further analysis of sequence identification number 25 shows the end of an open reading structure (1-43 bases), which encodes the last 13 amino acids of the TcaAiH peptide, and is disclosed here as the sequence identification number Number 35. Only 24 bases separate the end of the TcaAiU coding region from the beginning of the TcaBi coding region. Contained in the 24 bases are sequences that can be used as ribosome binding sites. Although possible, a promoter of the Bacillus gene is unlikely to be encoded in this short region. We assume that there are 3 long open reading architectures -84- This paper size is applicable to the Chinese National Standard (CNS) A4 specification (210X297 mm) (Notes for filling in this page)

1T 509722 A7 B7 V. Description of the Invention (82) [icaJ (Serial Identification Number 33), ica (Serial Identification Number 25, 65-36334 bases), and only borrowed 59 bases and ica The end of the sequence separated by sequence identification number 31)] is a operon, which has the transcription start upstream of the starting point of the icaJ gene (sequence identification number 33) and leads to the formation A polycistronic messenger RNA. Example 9 Screening of the gene library of the Bacillus solani gene encoding the TebA peptide peptide This example describes a method for identifying a DNA strain containing the TebAH peptide-encoding gene, isolation of the gene, and partial DNA salts Decision of base sequence. Primer and PCR reaction The TcbAn polypeptide of this insect active preparation is about 206 kDa. The amino acid sequence of the N-terminus of this peptide is disclosed as sequence identification number 1. Four groups of degenerate oligonucleotide primers ("forward primers": TH-4, TH-5, TH-6, and TH-7) were synthesized to encode a portion of this amino acid sequence, as in the example Described in 8 and shown below. Table 12 Printed by the Consumer Cooperatives of the Central Bureau of Standards, Ministry of Economic Affairs (I read the precautions on t before filling this page) Amino acid Phe lie Gin Gly Tyr Ser Asp Leu Phe TH-4 5 '-TT (T / C) ATI CA (A / G) GGI TA (T / C) TCI GA (T / C) CTI TT-3' TH-5 5, -TTCT / C) ATI CA (A / G ) GGI TA (T / C) AG (T / C) GA (T / C) CTI TT-3 'TH-6 5, -TT (T / C) ATI CA (A / G) GGI TA (T / C ) TCI GA (T / C) TT (A / G) TT-3 'TH-7 5, -TT (T / C) ATI CA (A / G) GGI TA (T / C) AG (T / C) GA (T / C) TT (A / G) TT3'- In addition, one primary ("a") and one primary ("b") sequence of one endopeptide preparation (TcbAH-PT81) has been determined. The descriptions are the serial identification number 23 and serial identification number 24. _ ^ _ This paper size applies to the Chinese National Standard (CNS) A4 specification (210X297 mm) Printed by the Employees' Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs 509722 A7 B7 V. Description of the invention (83) 4 groups of degenerate oligonucleotides The primers ("forward primers" · TH-4, TH-5, TH-6, and TH-7) are similarly designed and synthesized with a reverse complement of the coding sequence (the coding sequence is identified by the number 23 peptide) A part of it is shown below. Table 13 Amino acid Thr Tyr Leu Thr Ser Phe Glu Gin Val Ala Asn TH-8 3, TGI AT (A / G) GAI TGI AGI AA (A / G) CT (T / C ) GT (T / C) CAI CGI TT (G / A) -5 'TH-9 3, TGI AT (A / G) TT (A / G) TGI AGI AA (A / G) CT (T / C) GT (T / C) CAI CGI TT (G / A) -5 'ΉΗΟ 3'TGI AT (A / G) GAI TGI TC (G / A) AA (A / G) CT (T / C) GT (T / C) CAI CGI TT (G / A) -5 'TH-11 3, TGI AT (A / G) TT (A / G) TGI TC (G / A) AA (A / G) CT (T / C ) GT (T / C) CAI CGI TT (G / A) -5 'The set of these primers was used in a PCR® reaction to amplify and encode the W-14 DNA of Corynebacterium radians prepared from Example 6 TcbAiii gene fragment. All PCR® reactions are performed using Ampl iWaxTM gemstones and other Perkin Elmer reagents and experimental procedures using "hot start" technology. Typically, a mixture of MgCl2, dNTP's, 10X GeneAmp® PCR Buffer II and primers (total volume 11 μ1) is added to a tube containing a single wax bead. [10X GeneAmp® PCR buffer is composed of 100 mM Tris_HCl, ρ · 8.3 and 500 mM KC1]. The tubes were heated to 80GC for 2 minutes and allowed to cool. A solution containing 10X GeneAmp® PCR Buffer II, DNA template and AmpliTaq® DNA polymerase was added to the top of the wax seal. After melting the wax seal and mixing the composition by heat cycle, the final reaction conditions (50 μ1 in volume) are: 10 mM Tris-HC1, ρΗ8.3; 50 mM KC1; 2.5 mM MgCl2; dATP, dCTP, dGTP, dTTP 200 μM each; single Forward primer group 1.25mM; single reverse primer group 1.25μM, 1.25 units of AmpliTaq® DNA polymerase and 170ng of template DNA. These reactions are placed in a thermal cycler (such as Example 8) and the following formula _-86- This paper size applies Chinese National Standard (CNS) A4 specification (210X297 mm) I " I ^ 0 (tfirst Read the precautions on the back of this page and fill in this page again) • Binding · Order 509722 A7 B7 V. Description of the invention (84) Operation: Table 14 Temperature time cycle repeats 94 ° C 2 minutes IX 94 ° C 15 seconds 55-650C 30 seconds 30X 72 ° C 1 minute 72 ° C 7 minutes IX 15 ° C constant (please read the precautions on the back before filling out this page)-loading · A series of amplification systems use this degenerate primer group at 3 different mixing temperatures (55G, 60G, and 65GC). The reaction at 65GC was not amplified by agarose gel electrophoresis. The reaction with a 60QC conjugation temperature and containing the TT-5 + TH-10 primers resulted in an amplification product with motility corresponding to 2.9 kbp. A smaller amount of the 2.9 kbp product was generated under these conditions with TH-7 + TH-10 primers. When the reaction was condensed at 55GC, these primer pairs produced more 2.9 kbp products, and this product was also generated by the TT-5-5 + TH-8 and TT-5-5T_11 primer pairs. Another ambiguous 2.9 kbp band is found in the column containing amplification products from primer pairs from TH-7 plus TH-8, TH-9, TH-10, or TH-11. In order to obtain sufficient PCR amplification products for selection and DNA sequence determination, 10 individual PCR reactions were set using TH-5 + TH-10 primers and performed using the aforementioned conditions with a 55% mixing temperature. All reactions were combined and the 2.9 kbp product was purified from an agarose gel by Qiaex extraction as previously described. The other sequences determined by the TcbAH internal peptide are disclosed here. The sequence is -87- This paper size applies the Chinese National Standard (CNS) A4 specification (210X 297 mm). Ordered by the Central Consumers Bureau of the Ministry of Economic Affairs and printed by the Consumer Cooperative. 509722 A7 ____B7_ 5. Description of the invention (85) Column identification number 21 and sequence identification number 22. As before, the degenerate oligonucleotides (reverse primers TH-17 and TH-18) were made to correspond to the sequence (please read the notes on the back before filling this page) reverse complement, which encodes these wins Part of the peptide is an amino acid sequence. Table 15 Taken from the sequence identification number No. 21 amino acid Met Glu Thr Gin Asn lie Gin Gin Pro TH-17 3'-TAC CTT / C TGI GTT / C TTA / G TAI GTT / C GTT / C GG-5 ' Table 16 Taken from the sequence identification number No. 22 Amino acid Asn Prolle Asn lie Asn Thr Gly lie Asp TH-18 3, -TT (A / G) GGI TAI TT (A / G) TAI TT (A / G) TGI CCI TAI CT (A / G) -5, degraded oligos TH-17 and TH-18 printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs were used for Amplification experiments using TT-4, TT-5, TH-6 or TT-7 primers as 5 '-(forward) primers. These reactions amplify products of approximately 4 kbp and 4.5 kbp, respectively. These DNAs were transferred from agarose gels to Nylon membranes, and amplified using the prepared 4-5 + 1111-10 primer pair. A 32P-labeled probe of the product was hybridized. The 4 kbp and 4.5 kbp amplified products strongly hybridized with the 2.9 kbp product. These results were used to construct a map of the peptide sequences within the TcbAH (as shown in Figure 3). The approximate distances between these primers are shown in Figure 3 in nucleotide units. DNA sequence of 2 ^ 9kbD reef segment. About 200ng of purified 2.9kbp fragment (prepared as above) was precipitated with ethanol and dissolved in 17ml of water. One half was used as a sequence template and the 25-pniol TD-5 group was used as a primer; the other half was used as a template with TD-10 as a primer. The sequencing reaction was performed as in Example 8. No reliable sequence was generated using this T10-10 primer group; however, the reaction generation of the T-5-5 primer group revealed the following order: -88- This paper size applies the Chinese National Standard (CNS) A4 specification (210X297 mm) 509722 A7 ___B7 V. Description of invention (86) Column:

1 AATCGTGTTG ATCCCTATGC CGNGCCGGGT TCGGTGGAAT CGATGTCCTC ACCGGGGGTT 61 TATTNGAGGG ANTNGTCCCG TGAGGCCAAA AANTGGAATG AAAGAAGTTC AATTTNTTAC 121 CTAGATAAAC GTCGCCCGGN TTTAGAAAGN TTANTGNTCA GCCAGAAAAT TTTGGTTGAG 181 GAAATTCCAC CGNTGGTTCT CTCTATTGAT TNGGGCCTGG CCGGGTTCGA ANNAAAACNA 241 GGAAATNCAC AAGTTGAGGT GATGGNTTTG TNGCNANCTT NTCGTTTAGG TGGGGAGAAA 301 CCTTNTCANC ACGNTTNTGA AACTGTCCGG GAAATCGTCC ATGANCGTGA NCCAGGNTTN 361 CGCCATTGG the basis of this sequence, A certain sequence primer (TH-21, 5'-CCGGGCGACGTTTATCTAGG-3 ') was designed to be complementary to the 120-139th base, and started towards the 5' end of the gel-purified 2.9 kbp encoding TcbAn PCR fragment (that is, TH -5 end). The determined sequence is shown below and compared with the N-terminal peptide sequence of the biochemical assay of TcbAn sequence identification number 1.

TcbA ^ 2.9 kbo PCRK sequence verification

Queue identification number 笫 1: F I Q G Y S D L F G--A

I I I I I I I I I I I

2. 9 kbp sequence GC ATG CAG GGG TAT AGT GAC CTG ΤΓΤ GGT AAT CGT GCT M QGYSDL FGNRA > From the homogeneity of the derived amino acid sequence and the biochemical tester, the 2.9 kbp PCR fragment obviously represents the TbW Coding area. This 2.9 kbp fragment was then used as a hybrid primer to screen the Corynebacterium W-14 gene body gene library prepared in Example 8 for the aggregates containing the gene encoding TcbAn. • 89- This paper size is in accordance with Chinese National Standard (CNS) A4 (210X297mm) (Please read the notes on the back first. Fill in--items before filling this page

、 1T printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 509722 A7 ____B7_ V. Description of the invention (87) Screening of light solid skin condensate base solid library Note: Please fill in this page again.) This 2.9 kbp gel-purified PCR fragment was shown at 32 pg using the Boehringer Mannheim High Prime labeling kit as described in Example 8. The membrane containing approximately 800 colony residues from the condensate gene bank was screened as described previously (Example 8), and positive colonies were streaked to obtain isolated colonies and re-screened. After multiple screening and characterization steps, three colonies (8A11, 25G8, and 26D1) were positive. The 1 ^ human "specificity probe did not hybridize to any of the 4 aggregates (which contained the icaf gene) identified in Example 8. DNA from aggregates 8A11, 25G8, and 26D1 was used to limit enzyme design. / // 、 公 〇 ^ // ## ;? {//// (alone or in combination with other) digestion, these fragments are separated on an agarose gel and transferred to a as described in Example 8 On the Nylon membrane, the membrane was hybridized with a 32P labeled probe prepared from the 4.5 kbp fragment (produced by amplification of the DNA of the Bacillus bacillus gene with TH-5 + TH-17 primers). 8A11 and The map produced by 26D1 condensate DNA is the same as that produced by similarly cut genomic DNA on the same membrane. It was concluded that condensate 8A11 and 26D1 are the correct representatives of the TcbAu coding segment of the gene. However, condensate 25G8 Contains a single mother 7 // fragment slightly larger than the DNA of the genome. This may be due to the position of the insert in the vector. The membrane hybridization analysis of the coded MA sequence condensate 26D15 showed 4.5 kbp Probe hybridizes to a single large fcM / fragment (greater than 9 kbp). This fragment is gel purified and faithful 8 is connected to the PBC KS (the price M / site to generate the plastid pBC-S1 / R1. DNA part of this plastid DNA sequence is borrowed, primers ___- 90- This paper size applies China National Standard (CNS) A4 specification (2 × 0 × 297 mm) Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 509722 A7 B7 V. Description of the invention (88) “primer walking” The carrier sequence from the side was determined. The procedure used is described in Example 8. Further sequences were generated by new oligonucleotide extensions designed from previously determined sequences. The determined DNA sequence and genes identified by other methods (here The disclosure is sequence identification number 11 which is described in Example 12 below.) When compared, complete homogeneity was found at nucleotides 1-272, 319-826, 2578-3036, and 3068-3540 ( The total number of bases is 1712). It was concluded that two methods can be used to identify DNA fragments encoding these TcbAu peptides. Analysis of the amino acid sequence of the derivative of Glycan Gene was confirmed as sequence identification number 11 The sequence of a DNA fragment encodes a protein The derived amino acid sequence is disclosed herein as sequence identification number 12. Many characteristics confirm the identity of the gene to the one encoding the TcbAu protein. The N-terminal peptide of the TcbAH (sequence identification number 1; Phe (Lie Gin Gly Tyr Ser Asp Leu Phe Gly Asn Arg Ala) is encoded as the 88th to 100th amino acid. The TcbAii internal peptide TcbAH-PT81 (a) (sequence identification number 23) is encoded as the 1065-1077 amino acid, and TcbAH-PT81 (b) (sequence identification number 24) is encoded as the 1571 -1592 amino acids. Furthermore, the endopeptide TcbAirPT56 (sequence identification number 22) is encoded as 1474-1488 amino acids, and the endopeptide TcbAu-PT103 (sequence identification number 21) is encoded as 1614-1639 Amino acids. It is clear that the only reliable strain of this gene, which encodes the TcbAu peptide, is an insecticidal protein preparation isolated from a strain of Corynebacterium luminifera W-14. The protein, such as the TcbAH peptide, is derived from a larger peptide -91. The size of this paper applies the Chinese National Standard (CNS) A4 specification (210X297 mm) --------- 0 — ^- ----- 1T ----- * Crazy (Please read the precautions on the back before filling this page) 509722 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs A7 B7 V. Invention Description (89) Cutting. This evidence is based on the fact that the N-terminal version of the TcbAu sequence identification number 1 is located at 261 bases (sequence number 87) of a larger open reading structure (sequence identification number 11). The fact that it is near the N-terminal amino acid) is provided. This reading structure begins with a nucleotide encoding the amino acid sequence Met GlnAsn Ser Leu (which corresponds to the TcbAiN-terminal sequence of the peptide), and is disclosed herein as the sequence identification number No. 6-6. TcbA is considered to be a TcbAu precursor protein. The relationship between tcjM, icdB and tcl? C genes should be closely linked to the icM gene and may be transcribed into a single mRNA (Example 8). This gene is carried by aggregates that do not contain 虻, and different aggregates are identified due to the screening of individual gene pools. However, comparison of the amino acid sequence encoded by the icawan and icaC genes with the ic plus gene showed a considerable degree of homogeneity. The amino acid sequence retention (Protein Alignment Mode of MacVectorTM Sequence Analysis Software, scored with matrix pam250, hash value = 2; Oxford Molecular Group, Campbell, CA) is shown in Figure 4. In the scoring lines of each graph in Figure 4, the upper tip Γ) indicates homogeneity or retained amino acid value, and the lower tip (v) represents non-homogeneity. This analysis shows the amino acid sequence of the TcbA peptide from residues 1739 to 1894 and the 441 to 603 amino acids of the TcaBi peptide (162 out of the total 627 amino acids of TcaB, the sequence Identification number 28) is highly homogeneous. In addition, TcbA's 1932 to 2459 amino acid sequences are highly homogeneous with TcaBH peptides' 12 to 531 amino acids (520 of the total 562 amino acids; sequence identification number 30). If you consider the TcbA peptide-92- This paper size is applicable to the Chinese National Standard (CNS) A4 specification (210X 297 mm) m · I —ϋ · ϋ ϋϋ · ϋι n * Λ (Please read the precautions on the back and fill in (This page)

、 1T 509722 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs A7 B7 ____ 5. Description of the Invention (9) (Serial Identification Number 12) consists of 2,505 amino acids, which are located near the C-terminus and total 684 Each amino acid is homogeneous with the TcaBi and tcaBH peptides. This homogeneity is aligned with the alignment of the putative TcaB preprotein (sequence identification number 26). A considerable gap within the homogeneity of TcbA coincides with the junction between the TcaBi and tcaBii portions of the TcaB preprotein. Obviously, the TcbA and TcaB gene products are evolutionarily related, and they are speculated to share some common functions within the bacillus.

Example 10 The characteristics of zinc metalloproteinases in the bacilliform broth broth made, classified, and purified by Jingbaiyu # Protease inhibition and classification analysis: Protease analysis uses FITC-casein dissolved in water as the substrate (0.08% final analysis Concentration) ° Proteolytic reaction was performed in 25 μ1 bacilliform broth in a suitable buffer for 1 hour at 25 GC (total reaction volume 1501). The samples were also analyzed in the presence and absence of dithiobutofurfurol. After incubation, an equal volume of 12% trichloroacetic acid was added to precipitate undigested protein. After precipitation for 0.5 hours and subsequent centrifugation, ΙΟΟμΐ supernatant was placed in a 96-well microtiter plate, and the pH of the solution was adjusted by adding an equal volume of 4N NaOH. The protein digestion was then quantified using a Fluoroskan II fluorescent disc reader at excitation and emission wavelengths of 485 and 538 nm, respectively. Protease activity was tested in a range from pH 5.0 to 10.0 (0.5 unit increments). The following buffers were used at a final concentration of 50 mM: sodium acetate (pH 5.0-5-6), Tris-HCl (pH 7.0-8.0-8), and bis-Tris propanol (pH 8.5-8-10). In order to identify the type of protease observed, the crude broth with different protease inhibitors (0.5 · 5 / μ1 final concentration) -93 · _ This paper size applies the Chinese National Standard (CNS) Α4 size (210x297 mm) I ------- 0 ^-(Please read the precautions on the back before filling out this page),?! 509722 A7 B7 V. Description of the invention (91), and then use the aforementioned subject matter to be tested at PH8.0. Determination. These protease inhibitors used include E-64 (L-trans-epoxypolyalkylaminaminylamino [4-,-guanidino] -butane), 3,4-digas isocoumarin , Leupeptin, pepstatin, astatin, ethylene glycol diamine tetraacetic acid (EDTA), and 1,10-phenanthroline O Protease analysis performed over a pH range showed that the protease did indeed exist, showing maximum activity at approximately pH 8.0 (Table 17). The addition of DTT had no effect on the protease. Crude broth was then treated with different protease inhibitors (Table 18). Treatment of the crude broth with these inhibitors as described above shows that when added at a final han of 50 pg, 1,10-fenansolin causes complete inhibition of all protease activity, which 105 mg = 100 μl 2 mg / 5μ2 in ml of crude broth solution. These data indicate that the most abundant proteases found in the bacilliform broth are zinc metalloproteinases. Table 17 Effect of pH on the protease activity found in the first day production of Corynebacterium radians (W-14 strain) I ------- t-coat ------ IT ------ * * (Please read the notes on the back before filling this page)

pH

Flu. Unit; percentage of activity printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 55677788990 3013 taxi 78 17 7994 soil 448 45 12965 soil 483 74 14390 soil 1291 82 14386 soil 1287 82 14135 soil 198 80 17582 soil 831 100 16183 soil 953 92 16795 760 96 16279 soil 1022 93 15225 soil 210 87 This paper size applies to the Chinese National Standard (CNS) A4 (210 X 297 mm) -94- 509722 A7 B7 V. Description of the invention (92) a Flu. Unit is fluorescent Units (maximum value is about 28,000; background value is about 2200) b Relative to the percentage of activity at the maximum value of ρΗ8 · 0 ~ Different protease inhibitor pairs, the phylloxamine and 篦 1 J are found in dH8 Protein Pro-Live Night-time Mysterious Pain Inhibitor _ Modified Flu. Unit a Percent Inhibition b 1. Gas inhibitory enzyme inhibition group T diacid white and white control 46104-amine egg egg sool on E-1,3 White stomach apricot DM tananin-based inhibitor group f-factor * c M bean-sugar-factor 0〇Qu rDr > 3: d_ IXd * no L〇551574702 02195044401 347 (33222 '9 9 9 3 I ------- 0 ^ 1 * · (Please read the notes on the back before filling this page) Member of the Central Standards Bureau of the Ministry of Economic Affairs Consumption cooperative prints a corrected Flu · unit = fluorescent unit-background value (2200 fluorescent units). B Percent inhibition of protease activity at ρΗ8 · 0. CInhibitor is dissolved in methanol. DInhibition The material was dissolved in DMSO. The separation of the metalloproteinase was performed by adding the dialyzed 10-80% ammonium sulfate precipitate to a Q Sepharose column as in Example 5 for bacillus toxins. The column It was equilibrated with 50mM Na2P04, ρΗ7 · 0. After extensive washing, a gradient of 0 to 0.5M NaCl was used to elute the toxin protein. Most biological activities and proteins were eluted from 0.15-0.45M NaCl However, most of the proteolytic activity is apparently present in the 0.25-0.35M NaCl fraction, and some activities are in the 0.15-0.25M NaCl fraction. The SDS-PAGE analysis of the 0.25--0.35M NaCl fraction appears to be approximately the same. The main peptide band of 60kDa. The 0 · 15-0. 25M NaCl separation part contains a similar 60kDa band but the relative protein concentration is low. The gel following this separation part is 95- ^ Paper size applies to Chinese National Standard (CNS ) A4 size (210X297 mm) " ~ Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 509722 A7 B7 V. Description of Invention (93) The filtration system uses a Superose 12 HR 16/50 column. As a result, there is a major spike that moves to 57.5kDa, which contains a borrowed SDS -PAGE analysis of a significant 58.5 kDa band (over 90% of total stained protein). This isolated part determined the protease to be a zinc metalloproteinase by other analysis of different protease inhibitors as described above. Almost all protease activities present in the bacilliform broth broth on the first day of fermentation corresponded to this approximately 58 kDa zinc metalloproteinase. In the secondary separation of zinc metalloproteinases, W-14 bacilliform broth was obtained for 3 days, and the protease activity was as described in Schmidt, TM, Bleakley, B. and Nealson, K.M. with gelatin Observed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). SDS analysis gel (running gel; 5 · 5 X 8cm) is made of 12 · 5% polypropylamine (40% concentrated solution of acrylamide / bis-polyacrylamide; Sigma Chemical Co., St. Louis, MO), where the final concentration of 0.1% gelatin (Biorad EIA-grade reagent; Richmond, CA) was incorporated by dissolving in water. The SDS-stacked gel (1.0 X 8cm) was made with 5% polypropylene, and also replaced with 0.1% gelatin. Typically, 2.5 pg of the protein to be tested was diluted in 0.03 ml of SDS-PAGE loading buffer without dithiobutanol (DTT) and loaded onto the gel. Proteins were electrophoresed in 0GC in SDS running buffer (Laemmli, U.K. 1970. Nature 227, 680) at 8 mA. After the end of the electrophoresis, the gel was washed in 2.5% Triton X-100 for 2 hours. The gel was then incubated at 37GC for 1 hour in 0.1 M glycerol (pH 8.0). After incubation, the gel was fixed and stained in 0.1% melamine-acetic acid-water (30:10:60, 〇〇. / ¥ 〇 1.1.80; · Sigma Chemical Co.) Overnight. The protease activity is considered as a light-colored area compared to the black and ammonium black stained background. Its paper size is in accordance with Chinese National Standard (CNS) A4 (210X297 mm). (Please read the precautions on the back before (Fill in this page) Packing · 509722 Printed by A7 __B7 of the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 5. Description of the Invention (94) Due to the breakdown of proteins and the spread of gelatin that is incorporated later. At least three different bands generated by proteolytic activity at 58, 41, and 38 kDa were found. The analysis of the activity of different proteases in the culture of W-14 on the 3rd day was performed using FITC-casein (0.02% final analytical concentration) dissolved in water as the substrate. Proteolysis experiments were performed with different protein fractions in a total volume of 0.15 ml, and incubated at 370 C in 0.1 MTris-HCl (pH 8. 0) for 0-0.5 hours. The reaction was stopped by adding an equal volume of 12% trigas acetic acid (TCA) dissolved in water. After incubation at room temperature for 0.25 hours, the sample was centrifuged at 10,000 xg for 0.25 hours, and a 0.10 ml portion was transferred to a 96-well microtiter plate. The solution was then neutralized by the addition of an equal volume of 2N sodium argon oxide, and then quantified at the excitation and emission wavelengths of 485 and 538 nm using a Fluoroskan II fluorescent disc reader, respectively. Activity measurement was performed using FITC-casein and different protease concentrations at 370C for 0-10 minutes. 1 unit activity is defined as the amount of enzyme required to generate 1000 fluorescent units per minute, and the specific activity is defined as the unit / mg protease. Inhibition studies were performed using zinc metalloproteinase, 1,10-fenansolin and N-rhamnose glycosyloxyhydroxyl hypophosphite) -Leu-Trp (methadone phosphate) and dissolved in 100% A concentrated solution of these inhibitors in ethanol and water is implemented. Concentrations are usually 10 mg / ml and 5 mg / ml for 1, 10-fenansolin and methadone diacetate, respectively. The final concentration of inhibitor is 0.5-1.0 mg / ml per reaction. Treatment of W-14 crude broth with 1,10_ fenansolin (an inhibitor of all metalloproteinases) on day 3 resulted in the complete removal of all protease activity, whereas methadone phosphate (a similar to thermolysin) Inhibitors; Weaver, L · H ·, Kester, W · R ·, -97- This paper size applies to China National Standard (CNS) 8% 4% (210X 297 mm) (Please read the precautions on the back before (Fill in this page) 4 items and 4 packs-11 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 509722 A7 B7 V. Description of Invention (95) and Matthews, BW 1977. J. Mol. Biol. 114, 119-132) Treatment resulted in approximately 56% reduction in protease activity. This residual proteolytic activity cannot be further reduced by increasing methadone phosphate. The protease of W-14 bare bacillus broth was purified as follows: 4.0 liters of broth was concentrated using an Ami con spiral ultrafiltration cartridge S1Y100 connected to an Ami con M-12 transition unit. The filtered material (3.8 litres) with natural protein of less than 100 kDa was concentrated to 0.375 litres using an Amicon spiral ultrafiltration cartridge SIΥ10 connected to an Amicon M-12 filter device. The indwelling substance contains a protein ranging in size from 10 to 100 kDa. This material was packed into a Pharmacia HR16 / 10 column, which was packed with a PerSeptive Biosystem (Framington) Poros® 50 HQ strong anion exchange packing which was equilibrated with 10 mM sodium scale buffer (ρΗ7.0). The protein was filled at a flow rate of 5 ml / min, and the unbound protein was washed away with buffer until a28Q = o.oo. Finally, the protein was eluted in 40 minutes using a NaCl gradient of 0. 1.0 M NaCl at a flow rate of 7.5 ml / min. The isolated fraction is analyzed for its protease activity (as before) and the active isolated fractions are pooled. The proteolytically active fraction was diluted with 50% (v / v) 1M sodium phosphate buffer (ρΗ7 · 0) and packed into a Pharmacia HR 10/10 Mono Q column equilibrated with 10 mM sodium phosphate. After washing the column with buffer until A28Q = 0.00, the protein was eluted in a NaCl gradient of 0-0.5M NaCl at a flow rate of 2.0 ml / min within 1 hour. The isolated fraction was analyzed for its protease activity. Their separated fractions with the highest amount of methadone phosphate-sensitive protease activity were pooled, which was due to the 41/38 kDa protease described later. These separated parts were issued -98 · This paper size is applicable to Chinese National Standard (CNS) A4 specification (210X 297mm) I ------- 0 ^-(* · Read the precautions on the back before (Fill in this page)

1T 509722 A7 B7 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 5. Description of the invention (96) Now eluted in the range of 0 · 15-0.25M NaCl. Isolated fractions containing the methadone phosphate-insensitive protease activity (the 58 kDa protease) were also pooled. These fractions were found to elute in the range of 0.25-0.35M NaCl. The isolated portion of the methadone-phosphate-sensitive protease was subsequently concentrated to a final volume of 0.75 ml with a 111 1 1 0 1 11 11 & 66 @ -15 centrifugal transition device at a 0 1 13 1 -5 1 & 1 1 1 2 1. This material was added to a Pharmacia HR10 / 30 column at a flow rate of 0.5 ml / min. The column was plugged with Pharmacia Sephadex G-50 equilibrated with 1WM sodium phosphate buffer (pH 7.0) /0.1M NaCl. . The fractions with the highest amount of methadone-sensitive protease activity were subsequently pooled and centrifuged in a Millipore Ultrafree®-15 centrifugal filtration unit Biomax-5K NMWL membrane. The aforementioned proteolytic activity analysis revealed that this substance has only methadone-sensitive protease activity. The methadone phosphate-sensitive protease (58 kDa protein) was pooled, concentrated in a Millipore Ultrafree®-15 centrifugal filtration device Biomax-5KNMWL, and further separated in a Pharmacia Superdex-75 column. The isolated fractions containing the protease were pooled. Analysis of purified 58 and 41/38 kDa purified proteases showed that although type 2 proteases were completely inhibited by 1,10-fenansolin, only this 41/38 kDa protease was inhibited by methadone phosphate. Further analysis of the crude broth revealed that the protease activity of W-14 broth in the first day was due to the 41/38 kDa protease, which accounted for 23 ° / of the total protease activity. On day 3, W-14 broth increased to 44 ° / 〇. Standard SDS-PAGE analysis performed to test protein purity and obtain amino-terminal sequences was performed using a 4-20% gradient MiniPlus SepraGels purchased from the Integrated Separation System (Natick, MA). • 99- (Please read the notes on the back before filling this page)

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、 1T This paper size applies Chinese National Standard (CNS) A4 specification (210X 297 mm) Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 509722 A7 ____ B7_ 5. Description of the invention (97) The protein for sequencing at the amine end is in After purification (described later), it was transferred to a PVDF membrane (ProBlott® Membranes; Applied Biosystems, Foster City, CA), developed with 0.1% amide black, cut out, and sent to Cambridge Prochem; Cambridge, MA for sequencing. Derived from the 58 (sequence identification number 45) and 41 / 38kDa (sequence identification number 44) protease derived from W-14 broth on the 3rd, the amino acid end sequences of the protease were DV-GSEKANEKLK (sequence Identification number 45) and DSGDDDKVTNTDIHR (serial identification number 44). The 41/38 kDa protease sequence shows multiple amine ends, each containing an additional amino acid that is removed by proteolysis. Examination of the first, second, third and fourth order of the 38 and 41 kDa polypeptides resulted in a deduction of the sequence shown previously and showed that the diprotease is homogeneous. Example 11, Part A was screened by a gene library of the Bacillus genus gene through the use of an anti-tamper-encoding antibody against TcbA. In parallel with the aforementioned sequence, appropriate detection and sequencing were performed with the TcbAH victory (sequence identification number No. 1). This sequence was performed by preparing a bacterial culture broth and purifying the toxin as described in Examples 1 and 2 above. Genomic DNA was isolated from a strain of Corynebacterium radians W-14 grown in Grace's insect tissue culture medium. The bacteria were grown at 28 C and 250 rpm in 5 ml culture medium in a 250 ml grienmeyer flask for about 24 hours. Bacterial cells from the 100m culture medium were precipitated at 500Og for 10 minutes. The supernatant was discarded and the cell pellet was subsequently used for the isolation of genomic DNA. -100- This paper size is in accordance with Chinese National Standard (CNS) Α4 specification (2 丨 〇 > < 297 mm) --------- clothing-(A read the precautions on the back before filling in this page)

, 1T 509722 Printed by A7 ____ _B7_ of the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 5. Description of the invention (98) The gene 0 was modified by using one of the CTAB methods described in Section 1.13 of Human 1131 ^ 61 (mentioned above). While being separated. The section entitled "Large CsCl Precipitation of Bacterial Genomic DNA" was followed to step 6. At this point, an additional gas-imitation / isoamyl alcohol (24 ·· 1) extraction was performed, followed by a phenol / aerodynamic / Isoamyl alcohol (25: 24: 1) extraction step and a final gas imitation / isoamyl alcohol (24: 1) extraction. The DNA was precipitated by the addition of 0.6 times the volume of isoamyl alcohol. The precipitated DNA It was wrapped around the end of a curved glass rod, briefly immersed in 70% ethanol as the final wash, and then dissolved in 3ml TE. The DNA concentration estimated by the optical density of 280 / 260nm was about 2mg / ml. Using this gene Somatic DNA, a gene library was prepared. About 50 pg of the somatic DNA was digested along the part. Subsequently, NaCl density gradient centrifugation was used to separate the size of the partially digested DNA fragment. Assay by agarose gel electrophoresis contained 12kb (Or larger) isolated portions of DNA fragments of average size are ligated into BluScript plastids (Stratagene, La Joua, 0 & 11 {〇 :: 1 ^ 3) and enter an E. coli 0115〇6 or 0ye10 The strain transformed it. Separately, the purified part of the protein was sent to The Biotechnology Fusion Oncology Center of the University of Cornison (Madison) to generate antibodies against these proteins. The material sent was a HPLC-purified fraction containing natural first and second bands that had been denatured at 65 ° C. Twenty pg of this was injected into each of the four mice. A stable, single-source antibody-producing fusion tumor cell line was recovered after spleen cells from an unimmunized mouse were fused with a stable myeloma cell line. Fusion tumor recovery. -101- This paper size is applicable to China National Standard (CNS) A4 specification (210X297 mm)-~ --------- 11 ^ 装-(A read first_note on the back Please fill in this page again for details),? Τ 4 509722 A7 B7 V. Description of the invention (99) Separately, the multi-source anti-system was produced by obtaining the first band protein purified by natural agarose gel (see Example 1). It was subsequently used to immunize a New Zealand white rabbit. The protein was cut from the natural agarose gel, the gel pieces were briefly heated to 65GC to melt the agarose, and then quickly emulsified with an adjuvant to Prepared. Freund's Complete Adjuvant For the first immunization, Frode's incomplete adjuvant was used for 3 additional injections at intervals of 1 month. Each injection containing 50 to 100 mg of protein (about 0.2 ml of emulsified band 1) was given multiple subcutaneous injections Enter the back of the rabbit. Serum was obtained 10 days after the last injection, and blood was collected for another 3 weeks per week. The serum complement was inactivated by heating to 56QC for 15 minutes, and then stored at -20GC. The single-source and multi-source antibodies It is then used to screen the gene bank for antigenic performance, and the antigen can be measured by epitope. Positive strains were detected on nitrocellulose filter colonies. One of these positive strains was analyzed for immune collapse. The analysis of these strains, defined by both immunosuppression and southern analysis, led to tentative discrimination of the 4 genomic regions. Printed by the Consumers' Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (please read the notes on the back before filling this page) The first area is a gene encoding the peptide named TcbAH. The entire DNA sequence of this gene was obtained. It was published as sequence identification number 11. The identification of the internal sequence of the sequence coding sequence identification number 1 was proved by the presence of the amino acid 88 of the sequence identification number 1 and the amino acid came from the open reading by the sequence identification number 11 Deduced amino acid sequence. This can be confirmed by referring to the sequence identification number No. 12, which is the deduced amino acid sequence generated by the sequence identification number No. 11. -102- This paper size applies to China National Standard (CNS) A4 (210X 297 mm) 509722 Printed by A7 B7 of the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 5. Description of the invention (100) The second area of the toxin peptide contains These paragraphs are called TcaBi, TcaBn and TcaC. After screening the gene pool with a multi-source antiserum, this second region of the toxin gene was identified by generating multiple strains of proteins of different sizes. 'All of these proteins are on the immune turn with the multi-source antibody. Cross-reaction, and ^ ... homogeneity was also found on a southern turn. Sequence comparison showed that they belonged to the gene complex named Fangyu. Regions of two other antibody toxin strains were also isolated in this multi-source screening. These regions produce proteins that cross-react with a multi-source antibody, and also share DNA homogeneity with these regions as determined by Southern transduction. Therefore, the extracellular protein gene of Corynebacterium radians appears to represent an evolutionarily related gene family. To further study the theory that the toxin peptides in this organism may be evolutionarily related mutations, two methods were implemented to test whether other strains of Corynebacterium radians had the relevant proteins. This pCR amplification of the genomic DNA and the immunocompression analysis using the single-source or multi-source antibodies were performed. These results show that the related proteins are bacillus radians wx_2, WX-3, WX-4, WX-5, WX-6, WX-7, WX-8, WX-11, WX-12, WX -15 and Generation of W-14 strain. 1. Case analysis of the toxin strain of the β part of the toxin strain. Further DNA sequencing was performed on plastids isolated from the E. coli strain described in part A of the example. The nucleotide sequence from the 3rd region of the E. coli strain shows that it is three tightly connected open reading frameworks located in this genome segment. This section is named icc, and it has 3 sections named Hua _ -103- 、, water is very good! General T country national standard (eNS) A4 specification (210X297 mm (please read the precautions on the back before filling this page)-Pack.

、 1T 509722 A7 B7 printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 5. Description of the invention (1G1) Serial ID No. 56, ICCC million ID No. 58 and ICCC Serial ID No. 60 . The tight connection between these open reading frameworks is examined by sequence identification number 56 (which has 93bp of the stop codon that was iced and the start codon of kcZ? [Sequence identification number 56-29- 2994 bases] and a review by sequence identification number 58 (which has 131 bp of the stop codon of 纟 cc million and iccC [4930-4932 bases of sequence identification number 58] On) while being exposed. This physical map is shown in Figure 6B. The deduced amino acid sequence from the iccJ open reading architecture revealed that the gene encodes a 105,459 Da protein. This protein was named TccA (sequence identification number 57). The first 12 amino acids of this protein correspond to the N-terminal sequence obtained from a 108 kDa protein (sequence identification number 8, previously identified as part of the toxin complex). The deduced amino acid sequence from the 虻 β open read architecture shows that the gene encodes a 175,716 Da protein. This protein was named TccB (sequence identification number 59). The first 11 amino acids of this protein corresponded to the N-terminal sequence obtained from a protein with an estimated molecular weight of 185 kDa (sequence identification number 7). Similarity analysis showed that the TccB protein was related to the following proteins: those identified as TcbA sequence identification number 12 (37% similarity is 28%), TcdA sequence identification number 47 (35% similarity to 28 ° /. Same) as the TcaB sequence identification number 26 (32% similarity to 26%). (Using this GAP calculation, Wisconsin Package Version 9.0, Genetic Computer Group [GCG], Madison Wisconsin) o • 104 · _ This paper size applies to China National Standard (CNS) A4 specification (210X 297 public directors) (Please read first Note on the back, please fill out this page again) 4 items to refill

1T 509722 A7 B7 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 5. Description of the invention (102) The deduced amino acid sequence of iccC shows that this open reading architecture encodes a 111,694 Da protein, the protein product is named TccC (sequence identification number 61). Example ϋ The characteristics of the strains of the first-handed strains are conferred as broken. The collection described here contains the strains of Bacillus spp., Where the strains are evaluated based on confirmed microbial traits, and the traits are Bacillus spp. And distinguish it from other enterobacteriaceae and heterobacteria (Farmer, JJ 1984. Bergey5s Manual of Systemic Bacteriology, Vol 1. Pp. 510-511. [Ed. Kreig NR and Holt, JG] Wi11iams & amp Wilkins, Baltimore; Akhurst and Boemare, 1988 Boemare eis / ·, 1993). These unique traits are as follows: Gram-staining negative bacilli, biological size of width 0 5_2μιη and length 2-10μπι, red / yellow colony pigment formation, presence of crystal inclusions, presence of catalytic enzymes, no ability to reduce nitrate, bioluminescence Sexual existence, with the ability to take up dyes from the growth medium, positive protease production, growth temperature range below 37GC, can survive in anaerobic conditions and have mobility (Table 20). References for E. coli, heterobacteria, and Bacillus strains are included in all runs for comparison. The results of this population are consistent with all strains belonging to the Enterobacteriaceae and the genus Corynebacterium. A photometer was used to confirm the bioluminescence of each strain and to provide quantitative and relative measurements of light production. Regarding the measurement of relative luminescence units, these broths from each strain (cells and media) were measured at 3 time intervals (6, 12 and 24 hours) after inoculation in liquid culture and compared with background luminescence (uninoculated Medium vs. water). Measure the light from different broths -105- This paper size is applicable to China National Standard (CNS) A4 specification (210X297mm) (Please read the notes on the back _ 4 first, then fill in and install —: write this page) Order • 噃Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 509722 A7 B7 V. Description of the Invention (103) Prior to radiation, the cell density was measured in a Gil ford Systems (Oberl in, 0H) spectrometer by using a sipper ceU. Absorbance (560nm) confirms this. Then perform appropriate dilution (to normalize the optical density to 1.0 units) before measuring the luminosity. The portion of the dilution broth is then placed in test tubes (300 μ1 per tube) and Interpreted by Bi〇-〇rbit 1251 photometer (Bio-Orbit 0y, Twiku, Pinland). The integration time for each sample was 45 seconds. These samples are continuously mixed (rotated in a test tube with a throttle plate) during interpretation to provide gas utilization. A positive test was determined to be 5 times or more the background luminescence (about 5-10 units). In addition, the luminescent properties of the colonies were measured by covering with photographic films and examining the films in a dark room. These Gram staining characteristics of each strain were confirmed by combining the commercial Gram staining kit (BB1, Cockeysviile, MD) with Gram staining control slides (Fisher Scientific, Pittsburgh, pA). . Microscopy followed by Zeiss microscope (Carl

Zeiss, Germany) was implemented with a 100X oil lens (with 10X eyepiece and 2x lens magnification). Microscopic examination of biological size, cell characteristics, and inclusion bodies (the latter after logarithmic growth) of individual strains uses wet mount slides (1 OX eyepieces, 2X lens magnification, and 4 × objective lens) with oil and Phase difference microscopy with micrometer (Akhurst, RJ and Boemare, NE 1990. Entomopathogenic Nematodes in Biological Control "ed · Gaugler, R. and Kaya, H.]. Pp. 75-90. CRC Press, Boca Raton, USA .; Baghdiguian S ·, Boyer-Giglio, M ···, Thaler, JO ·, Bonnot, G., Boemare, N · 1993 · Biol · Cell 79, 177- -106- Paper Standards are applicable to China National Standard (CNS) A4 specifications (210X 297 mm I -------- φ-batch clothing-m * (Please read the precautions on the back before filling out this page) Order Central Printed by 509722 A7 B7, Standards Bureau Consumer Cooperative V. Invention Description (104) 185). Colony pigmentation was observed after inoculation on Bacto Nutrition Agar (Difco Laboratories, Detroit, MI; prepared as labelled). Inoculation was performed at 28GC, These features are generated after 5_7 days To test for the presence of the enzyme-catalyzed enzyme, a colony of the test organism was prepared from a nutrient agar plate and placed on a small plug, and placed on the bottom of a glass test tube. 1 ml of a household argon peroxide solution was gently moved along the side of the tube. Add. When a bubble (assuming oxygen) appears immediately or within 5 seconds, a positive reaction is recorded. A control group of uninoculated nutrient agar and hydrogen peroxide solution is also tested. To test for nitrate reduction, each culture The material was inoculated in 10 ml of Bacto nitrate broth (Difco Laboratories, Detroit, MI). After incubation at 28 ° C for 24 hours, the formation of nitrite was achieved by adding 2 drops of sulfanilic acid reagent and 2 drops of α- 2-naphthalene Tested with amine reagents (see Difco Manual, 10th edition, Difco Laboratories, Detroit, MI, 1984). A unique pink or red production indicates nitrite formation from nitrate. The ability of each strain to take up dye from the growth medium Bacto MacConkey agar containing neutral red dye, Bacto Tergi to 1-7 agar containing bromine thyme pan blue dye, and Bacto EMB agar containing eosin-Y dye (agar from Difco Laborator ies, Detroit, MI, all prepared according to label instructions) Test it. After inoculation on these media, dye uptake was recorded after incubation at 28GC for 5 days. Growth on these latter media is characteristic of members of the Enterobacteriaceae. The motility of each strain was prepared using Bacto Motility Test Medium (Di fco

Laboratories, Detroit, MI) solution was tested. Each strain was subjected to bottom puncture inoculation, and the motility spread out through the inoculation line to spread out the growth -107- This paper size applies to the Chinese National Standard (CNS) A4 specification (210X297 mm) --------- -^ 装 ------ Order ------ • * (Please read the precautions on the back before filling out this page) 509722 A7 _B7 5. The invention description (1G5) area was judged microscopically. In many cases, motility is also observed microscopically from liquid cultures under wet wipes. The biochemical nutrition assessment of each strain was performed using BBL Enterotube II (Benton, Dickinson. Germany). Product instructions are followed by the exception of 28GC which lasted for 5 days. The results are consistent with previously cited reports for Corynebacterium. The protease was produced by using a Bacto gelatin plate (Difco Laboratories, Detroit, MI) made as described on the label to observe the hydrolysis of the gelatin. Cultures were inoculated and the plates were grown at 28QC for 5 days. To evaluate growth at different temperatures, the agar plate [2% No. 3 protein-glycoprotein dissolved in deionized water, printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs, printed glands and 2% Bacto-Agar (Difco Laboratories, Detroit, MI) ] Lined with a common source of inoculum. Plates were sealed with Nesco® sheets and incubated at 20, 28 and 37GC for up to 3 weeks. No growth was seen in the 37QC discs. No cell survival was seen after one week in the 28QC incubator. The oxygen demand of the Bacillus strain was tested as follows. A bottom puncture into fluid thioglycolate broth (Difco, Detroit, MI) was performed. The tubes were incubated at room temperature for 1 week, and the culture was then examined for patterns and extent of growth. The resazurin indicator shows the degree of oxidation of the culture medium or the aerobic living area (Difco Handbook '10th Edition, Difco Laboratories, Detroit, MI). The experimental results of the growth zone obtained from the tested bacillus strain were consistent with those of a facultative anaerobic microorganism. _Taxonomic Traits of Corynebacterium Strains Evaluated Traits *

Strain | A | B c D E | F G | H I J K L M N 0 P Q -108- This paper size it financial standard (CNS) A4 specifications (21GX297 mm 7 509722 Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs A7

«. ο Limb, luminescent, D = cell shape, E = motility, F = nitrate reduction, G = presence of catalytic enzymes, H = gelatin hydrolysis, 1 = dye uptake, j = pigmentation, £ = in service 8 Growth on agar, L = growth on MacConkey agar, shame for growth on 丨 Qie 7 agar, N = facultative anaerobic, 0 = growth on 20QC, P = • 109 on 28GC -This paper size is applicable to China National Standard (CNS) A4 specification (210X 297 mm) (please read the notes on the back of the asset before filling out this page) • Packing.-Order 509722 A7 B7 Printed by the Central Consumers Bureau of Ministry of Economic Affairs System V. Description of the invention (107) Growth, Q = Growth at 37GC, +-/ + = Positive or Negative, rd = Bacilli, S = Size within the genus description range, Ro = Red- Orange, LR = light red, R'tc, 0 = orange, Y = yellow, T = brown, LY = light yellow, YT = yellow brown, L0 = light orange. Cell fatty acid analysis is a recognizable tool for characterizing bacteria at the genus and species level. (Tornabene, TG · 1985 · Lipid Analysis and the Relationship to Chemotaxonamy in Methods in Microbiology, Vol · 18, 209-234 ·; Goodfellow, M · and O'Donnell, A · G · 1993 · Roots of Bacterial Systematics in Handbook of New Bacterial Systematics (ed. Goodfel low, M. & O'Donnell, AG pp. 3-54. London: Academic Press Ltd.), these references are incorporated herein by reference and are used to confirm our The collection of waits is relevant at the level of the genus. The culture was shipped to a foreign contracting laboratory, and a microbe ID (MIDI, Newark, DE, USA) microbiological identification system (MIS) was used for fatty acid methyl ester analysis (FAME). The MIS system consists of a Hewlett Packard HP5890A gas chromatograph equipped with a 25 Li X 0.2 mm 5% toluene-based fused silica capillary column. Argon is used as a carrier gas and a flame ionization detector has the function of connecting with an automatic sampler, integrator and computer. The computer compares the fatty acid methyl ester of the sample with a fatty acid database of a microorganism and uses a calibration mixture of known fatty acids as a control. As selected by the contracting laboratory, the pre-analytical strain was grown on trypsin soy agar at 28GC for 24 hours. The extraction of the sample was performed by the contracting laboratory in accordance with the standard FAME methodology. No direct identification of these strains has been performed on other luminescent bacteria groups other than Bacillus spp. • 110- This paper size applies Chinese National Standard (CNS) A4 specifications (210X297 mm) (Please read the precautions on the back before filling in this (Page) 4 • Fill in the items again. Binding and ordering printed by the Consumer Cooperatives of the Central Bureau of Standards of the Ministry of Economic Affairs 509722 A7 B7 V. Description of the invention (108). When the analysis of the group is performed (which compares the fatty acid image of a group of isolates), the fatty acid image of the strain is related at the genus level. The evolutionary ambiguity of these Bacillus strains in our collection was measured using the genomic DNA from each strain by analysis of the fingerprinting of the genomic DNA of the PCR (polymerase chain reaction) vector. . This technique is based on the fact that families of repeated DNA sequences are ubiquitous in the genes of disparate strains (by Versalovic, J., Schneider, M., DE Bruijn, FJ and Lupski, JR 1994. Methods Mol. Cell. Biol., 5, 25-40. (Rereview). Three of these, the repeated extragenic palindromic sequence (REP), the intestinal bacteria repeat intergenic consensus sequence (ERIC), and the BOX factor are considered to play an important role in the composition of the bacterial genome. The composition of the genome is believed to be shaped by selecting the different distributions of these factors in the genes of closely related strains, which can be used to distinguish these strains (such as Louws, F.J., Fulbright, D.W., Stephens, CT and DE Bruijn, FJ 1994. Appl. Environ. Micro. 60, 2286-2295). Rep-PCR uses oligonucleotides complementary to these repeats to amplify Guan A fragments of different sizes in between. The obtained products were separated by electrophoresis to establish the DNA fingerprint of each strain, the genomic DNA was isolated from our strains, and the cell pellet was resuspended in TE buffer solution Tris-HCl, ImM EDTA, ρΗ8 0) to 10ml

To a final volume, 12 ml of 5M NaCl were subsequently added. This mixture was centrifuged at 15, OOOxg for 20 minutes. The resulting precipitate was resuspended in 5.7 ml TE and added with 300 μ1 10% SDS and 60 μ1 20 mg / ml proteinase K (Gibco BRL -111- This paper is for Chinese national ladder Standard (CNS) A4 specification (2ι〇χ297 public director) --------- ^-install-* 0 (Please read the precautions on the back before filling out this page) Order the staff of the Central Bureau of Standards of the Ministry of Economic Affairs Printed by the cooperative 509722 A7 __B7_ V. Description of the invention (109)

Products, Grand Island, NY). This mixture was incubated at 370c for 1 hour, followed by the addition of 10 mg of lysozyme for 45 minutes. Subsequently, 1 ml of 5M NaCl and 800 μl of CTAB / NaCl solution (10% w / v CTAB, 0.7M NaCl) were added, and the mixture was incubated at 65QC for 1 hour, gently mobilized, and then incubated and stirred for 20 minutes to promote the cellularity. Material clarification. Add an equal volume of aerosol / isoamyl alcohol solution (24: 1, v / v) and mix gently and centrifuge. Extraction was then performed twice with an equal volume of phenol / aerobic / isoamyl alcohol solution (50: 49: 1). Genomic DNA was precipitated with 0.6 volumes of isoamyl alcohol. The precipitated DNA was removed with a glass rod, washed twice with 70% ethanol, dried and dissolved in 2 ml STE (10 mM Tris-HCl, pH 8.0; 10 mM NaCl, 1 mM EDTA). The DNA was then quantified by an optical density of 260 nm. The following primers were used for rep-PCR analysis of the bacillus genomic DNA: REP1R-I; 5, -IIIICGICGICATCIGGC-3 'and REP2-I..5, -ICGICTTATCIGGCCT-AC-3'. The PCR was performed using the following 25μ1 reaction: 7.75μ1 Η20, 2.5μ1 10 × LA buffer (PanVera Corp ·, Madison, WI), 16μ1 dNTP (2.5mM each), 1μ 每种 each (50ρM / μ1) 1 μΐ DMSO, 1.5 μ1 genomic DNA (concentration range 0.075-0.48 (^ g / pl) and 0.25 μ1 TaKaRa EX Taq (PanVera Corp., Madison, WI). The PCR amplification system uses the following conditions in a Perkin Elmer DNA Thermal Cycler (Norwalk, CT): 950C / 7 minutes, followed by 35 cycles of 94GC / 1 minute, 44GC / 1 minute, 65GC / 8 minute, and then 15 minutes at 65QC. After the cycle, the 25μ1 reaction 5μ1 6 × gel filling buffer (0.25% bromophenol blue in Η20, 40 ° / .w / v sucrose) was added. Then a 15 X 20cm 1 ° /. Agarose gel was taken _ -112- This paper Standards are applicable to China National Standard (CNS) A4 specifications (210X297 mm) ----------- (t read the precautions on the back of t before filling out this page)-order 509722 employee consumption of the Central Standards Bureau of the Ministry of Economic Affairs Cooperative printed A7 B7 V. Description of invention (no) 8μ1 of each reaction in TBE buffer (0.09M Tris-borate, 0.002M EDTA) Internal gel electrophoresis. The gel was electrophoresed at 45v for about 16 hours. The gel was then stained in 20pg / ml ethidium bromide for 1 hour and decolorized in tbe buffer for about 3 hours. The gels were then photographed under UV lighting Polaroid® photos. The presence or absence of each strain in a specific size band was evaluated from these photos and entered in a similarity matrix in a numerical taxonomy software program (NTSYS-pc; Exeter Software, Setauket, NY). The control group of Escherichia coli HB101 and a control group of lanthoinonas oryzae pv. Oryzae were analyzed at the same time to generate a PCR fingerprint that is consistent with the published report. , 1 (〇6111: 11, 1 \ & 11 (11 叩 31 ^,: ^. 1991 ·

Nucleic Acid Res. 19, 6823-6831; Vera Cruz, CM, Halda-Alija, L., Louws, F., Skinner, DZ, George, ML, Nelson, RJ, DEBruijn, FJ, Rice, C. and Leach, JE 1995. Int. Rice Res. Notes, 20, 23-24; VeraCruz, CM, Ardales, EY, Skinner, DZ, Talag, J. Nelson, RJ, Louws, FJ, Leung, H., Mew, TW and Leach , JE 1996 Phytopathology, published separately). The data from Bacillus bacillus were then analyzed by a series of programs in NTSYS-pc (as follows): SIMQUAL (similarity of qualitative data) to generate a matrix of similarity coefficients (using Jaccard coefficients); SAHM cluster (Sequential, Agglomerative, heirarchical and Nested clustering) [Using UPGMA (Unweighted Pair-Group Method with Arithmetic Averages) method] can classify related strains and can be represented by an evolutionary tree (Figure 5). C0PH (cophenetic -113- This paper size is applicable to Chinese National Standard (CNS) A4 size (210X297 mm) (Please read the precautions on the back before filling out this page) " 0 " installed · • item then Fill in too

、 1T 509722 Printed by A7 B7_, Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs. 5. The invention description (in) values) and MXC0MP (matrix comparison) programs were used to generate a matrix of phenotypic values and compare the values between this value and as Correlation between the underlying raw matrices of the cluster. As a result, a normalized Mantel statistical value (. Is generated, which is a measure of good fit for a cluster analysis (r = 0 · 8-0 · 9 represents _excellent fit). In our example r = 0.919. Therefore, our collection contains representative strains of this bacillus genus that are divergent and easily distinguishable. Example 13 The toxin produced by different bacillus strains ^ The original "bacterial" culture lines of different bacillus strains A primal mutant secondary selection was inoculated to 175 ml of a 2% No. 3 protein glycoprotein gland (PP3; Difco Laboratories, Detroit, MI) liquid medium, which was contained in a 500 ml three-way junction sealed with Kaput and one of Diron's neck In a flow bottle. The inoculum for each strain is derived from an agar slant culture or a plate culture. After inoculation, these bottles are incubated on a rotary stirrer at 150 rpm at 280C for 16 hours. These bacteria This culture was then used as a uniform inoculum source for fermentation of each strain. In addition, the log culture after the log period was covered with sterile mineral oil, a sterile magnetic stir bar was added for subsequent resuspension, and the Train The material is stored in a dark place at room temperature to provide long-term storage of the inoculum in a toxin-competent state. The resulting broth is supplemented by adding 1% of the active growing culture of the bacteria to a fresh 2% PP3 medium (For example, adding 1.75ml per 175ml of fresh medium) and inoculated. The broth is produced in a 500ml three-way throttle bottle or a 2800 convex bottom throttle bottle (500ml volume) covered with silicone foam. Incubate under the aforementioned conditions for 24-28 hours. After incubation, the broth is poured into a -114- This paper size applies to China National Standard (CNS) A4 specifications (210X297 male thin 1 (Please read the precautions on the back before (Fill in this page) 4 refills · Packing ·-Renewal · 509722 Printed by the Consumers' Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs A7 B7 V. Description of the invention (112) Sterilized in a 1L polyethylene bottle at 2600xg at 10QC 1 Hours and separated from the cell and debris sediment. The liquid broth was then filtered through Whatman GF / D (2.7 pM retention) and GF / B (1.0 μM retention) glass filters to remove debris. Further broth Clarify the system to make use of All lines of a 5μ open-channel filter are flowed to a microfiltration device (Pall Filtron, Northborough, MA). If necessary, additional clarification can be achieved by cooling the broth (to 4GC) at 2600xg Centrifuge for several hours. Following these procedures, the broth was sterilized by filtration with a 0.2 μM nitrocellulose membrane filter. Sterile broth was then used directly for biological analysis, biochemical analysis or 10,000MW to block M12 An ultrafiltration unit (Ami con, Beverly, MA) or a centrifugal concentrator (Millipore, Bedford, MA and Pal 1 Filtron, Northborough, MA) with a pore size of 10,000 MW to concentrate it. In the example of a centrifugal concentrator, the broth was centrifuged at 2000 xg for about 2 hours. The 10,000 MW permeate is added to the corresponding retentate to achieve the desired composition concentration of greater than 10,000 MW. The inactivated heating broth samples were heated in a heat block filled with sand and heated at 100 GC for 10 minutes. These broth and toxin complexes derived from different strains of Coryneform bacteria are useful for reducing insect populations and are used in a method of inhibiting an insect population, which comprises applying an effective insect inactive amount of the active substance In an area of the insect. The breadth of insecticidal activity observed in broths fermented by a group of selected Bacillus strains as described above is shown in Table 20. Other insecticidal activity may be increased by increasing the concentration of the broth or should be -115- This paper size is applicable to China National Standard (CNS) A4 specifications (2 丨 〇 > < 297 mm) ------- --II * · (Please read the precautions on the back before filling this page) Order printed by the Central Consumers Bureau of the Ministry of Economic Affairs and printed by the Consumer Cooperative 509722 A7 _____B7 _ V. Description of the invention (113) Different fermentation methods are used in these strains. Measured. Consistent with the activity associated with a protein, the insecticidal activity of all the strains tested was athermal (see above). Culture broths from different Bacillus strains showed different insecticidal activity (mortality and / or growth inhibition, reduced adult hatching) against a variety of insects. More specifically, this activity is seen against corn rootworm larvae and cotton weevil larvae, which are members of the insect Coleoptera. Other members of the Coleoptera include a beetle, a pollen beetle, a flea beetle, a seed beetle, and a Colorado potato beetle. Activity has also been observed to fight against Spodoptera spp. And corn plant jumping worms, which are members of the homoptera order. Other members of the homoptera order include plant worms, pear wood winds, fruit-like trematodes, scale insects, whiteheads, blowworms, and many host-specific aphid species. These broths and purified toxin complexes are also active against tobacco budworms, tobacco hornworms, and European corn perforators, which are members of the order Lepidoptera. Other typical members of this order are beet armyworm, Korean cabbage moth, brown sugar moth, tobacco cottonworm, apple moth, tapeworm, Indian rice moth, leaf roller, cabbage bug, cotton moth, sleeping bagworm, oriental tent-like Caterpillars, lawn networms, and autumn night moths. Activity is also seen against fruit flies and mosquito larvae, which are members of the order Diptera. Other members of the Diptera order are pea maggots, carrot flies, cabbage root flies, root grape flies, onion flies, long-leg flies, house flies and different mosquito species. The activity of the broth and toxin complex has also been shown to combat the two-spotted spider maggots. It contains the spider maggot maggot, broad maggots, red maggots, red maggots, and pear tree disease. Ascaris and member of the order Ascaris of Ascaris lumbricoides. -116- This paper size applies to Chinese National Standard (CNS) A4 (210X297mm) ---------------, possible ----- · «(Please read the Please fill out this page again} Printed by the Consumer Cooperatives of the Central Bureau of Standards of the Ministry of Economic Affairs 509722 A7 __; ___ B7__ V. Description of the Invention (114) The activity against corn rootworm larvae was tested as follows. The bacilliform culture broth (0- 15 times concentrated, filtered and sterilized), 2% No. 3 protein glycoprotein gland, purified toxin complex, 10 mM sodium phosphate buffer solution (ρΗ7 · 0) were directly applied to the surface of artificial feed in 40 μ1 portions (about 1 5cm2) (Rose, R.I. and McCabe, JM 1973. J. Econ. Entomol. 66, 398-400). The toxin complex was diluted with 10 mM sodium phosphate buffer (pH 7.0). The wells were air-dried in a sterile operation box, and the wells were placed in a single, new, Diabrotica undecimpunctata (Southern Corn Root Worm, SCR) that was dissolved by the surface Tibetan Mastiff. The trays were sealed and placed in a humid growth box. 27GC was maintained for an appropriate period of time (3-5 days). Mortality and larval weight were subsequently recorded. Usually, all studies Each treatment used 16 insects. The mortality rate in the control group was less than 5%. The activity against cotton weevil (such as Acwonas ·) was tested as follows. Concentrated (1-10 times) bacilliform broth, control medium ( 2% of No. 3 protein glycoprotein peptone), purified toxin complex (.23mg / ml) or 10mM sodium phosphate buffer (ρΗ7.0 ·) was applied to 0.35g artificial feed (Stoneville Yellow) in 60μ1 aliquots. Coleoptera feed) and allowed it to dry. A single, 12-24 hour cotton weevil larva was placed on the feed, and the wells were sealed and kept at 25QC, 50% RH for 5 days. Mortality and The weight of the larvae was subsequently evaluated. The mortality rate in the control group ranged from 0 to 13%. The activity against mosquito larvae was tested as follows. The analysis was performed in a 96-well microtiter plate. Each well contained 200 μl of an aqueous solution (10 Concentrated bacilliform culture broth, control medium (2% No. 3 protein glycoprotein gland), 10 mM sodium dish buffer (ρΗ7.0), toxin complex (0.23 mg / ml) -117-present Paper size applies to China National Standard (CNS) A4 (210 X297 mm) -------------- IT ----- * 璧 (Please read the notes on the back before filling this page) 509722 A7 B7 V. Description of the invention (η5) or Water) was applied to 0.35 g of artificial feed (Stoneville Yellow Coleoptera feed) and about 20 1-day-old larvae (e.g., 5 ae along) in 60 μl aliquots. There are 6 wells for each treatment. These results were interpreted 3-4 days after vaccination. The mortality rate in the control group ranged from 0 to 20%. The activity against Drosophila was tested as follows. The purchased Drosophila culture medium was prepared using 50% dry medium and 50% liquid. The liquid was water, a control medium (2% No. 3 protein glycoprotein gland), a 10-fold concentrated bacilliform culture broth, and purified Toxin complex (0.23 mg / ml) or 10 mM sodium phosphate buffer (ρΗ7.0). This is done by placing 4.0 ml of dry medium in each of the three feeding bottles of each treatment and adding 4 ml of the appropriate liquid. Ten sisters of fruit ropes were then added to each 25ml bottle. The bottles were placed on an experimental table at room temperature and under fluorescent light. Pupae or adults were counted 15 days after exposure. Adult hatching compared with water and control medium (decrease by 0-16%). Printed by the Consumer Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs (please read the notes on the back before filling this page). The activity against adult worms (ifecros 纟 e / es and corn plant A) (Peregrinus maudis) is the same. Tested by ingestion assay, the analysis is designed to allow the ingestion of the active substance without any external contact. The active / "food" solution container is borrowed from the center of the bottom of a 35 X 10mm petri dish It was made by drilling 2 holes. A 2-inch pair of Parafilm M® squares was placed on the orphan and secured with a ring. A 1-ounce plastic cup was then placed into about 7 jumping worms and the container was placed in the Above the cup (Parafilm face down). The test solution was then added to the container through the hole. In the test using a 10-fold concentrated bacillus fungi culture broth, the broth was compared with the control medium (2% 3rd No. glycoprotein gland) was dialyzed with 10 mM sodium phosphate buffer solution (pH 7.0), sucrose (to 5 ° / 〇) -118-This paper size applies Chinese National Standard (CNS) A4 specification (210X297 mm) ^ " 509722 A7 B7 Central Standard of the Ministry of Economic Affairs Printed by the Bureau ’s Consumer Cooperatives. 5. Description of the Invention (116) was added to the resulting solution to reduce the mortality of the control group. Purified toxin complex (0.23 mg / ml) or 10 mM sodium phosphate buffer (P7. 〇). ) Were also tested. Mortality was recorded on day 3. The analysis was placed in a 28GC, 70% RH, 16/8 photoperiod incubator. These analyses scored mortality at π hours. Control group The mortality rate is less than 6%. The activity against Lepidoptera larvae was tested as follows. Concentrated (10 times) broth culture broth, control medium (2% No. 3 protein glycoprotein gland), purified toxin complex ( 0.02 mg / ml) or 10 mM sodium acid buffer (ρΗ7.0) was directly applied to the surface of a standard artificial lepidopteran feed (Stoneville Yellow feed) (about ι · 5〇η2) in 40 μ1 aliquots. The feed tray It was air-dried in a sterile operation box, and each well was put into a single newborn larva. The eggs of European corn perforator and tobacco horn (Sezia) were obtained from commercial sources and hatched indoors, however ffeHothis virescens The equipment is supplied by U Bank. After putting in the larvae, the feed trays were sealed, placed in a humid growth box and maintained at 270C and darkness for an appropriate period of time. On the fifth day, the mortality and larval weight were recorded. Generally, 16 insects were used for each treatment in all studies. The mortality of the control group usually ranges from about 4 to about 12.5% for the control medium and less than 10% for phosphate buffered saline. TetranycAus urt / cae activity was tested as follows. Young pumpkin seedlings were pruned to a single cotyledon and concentrated in a 10-fold broth, control medium (2% No. 3 protein glycoprotein gland), purified toxin complex (0.23 mg / ml), 10 mM sodium phosphate buffer (PH7. 0) sprayed to the liquid stream. After drying, these plants are a mixed family of spider maggots ____ -119- This paper size applies to the Chinese national 'CNS) A4 size (210X 297 mm) (Please read the precautions on the back before filling this page) — • I refill

1T 509722 A7 B7 V. Explanation of the invention (in) group infection, which lasted 72 hours under the temperature and humidity of the laboratory. Surviving roundworms were then counted to determine levels in the control group. (Please read the notes on the back before filling this page) Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 20- 11 This paper size is applicable to Chinese National Standard (CNS) A4 (210X 297 mm) 509722 V. Description of the invention (11S)

Table 20 Derived from the broth of different stalk makeup and stuffing sticks ^ Observed hiding A M. The worm species with sensitivity 1 WX -1 WX-2 WX-3 WX-4 WX-5 WX- 6 WX-7 WX-8 WX-9 WX-10 WX-11 WX -12 WX-14 WX-15 W30 NOl WIR HP88 Hb Hm H9 [14 ATCC 43948 ATCC 43949 ATCC 43950 ATCC 43951 ATCC 43952 32, 4, 5, 6, 7, 8 2.4 1.4 4 3, 4, 5, 6, 7, 8 1.2.4 1.2.4 4 ο QJ 11 8,88, 9 9 9 7 8 7 7 8 Cii8 87, ccr6, 9 fff, J , 7 5778555 6,84,6,5,7,4,4,4, 45,445,3,5,4,5333, 2,4,2,2,42334222 li 1i li oo lx oxw 11 oo IX lx lx IX It it ft ft

Printed by the Central Bureau of Standards, Ministry of Economic Affairs, for consumer cooperation. 1 The mortality and / or growth inhibition is 25% or more compared with the control group. 2: Tobacco budworm; 2: European corn perforator; 3: Tobacco hornworm; 4; Southern corn rootworm; 5: Cotton weevil; 6: Mosquito; 7: Drosophila; 8: Star-shaped jumping worm; 9: Maize plant worm; 10: Two-spot spider tapeworm. -121-Τ '---____ 1Z1 The paper size is applicable to the Chinese National Standard (CNS) A4 (210 X 297 mm) 509722 Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs A7 __B7 V. Description of Invention (ll9) Example 14 _Non ~ -14 strains of Coryneform bacteria: Purification, characterization of activity spectrum purification This experimental procedure (below) is similar to that developed for the purification of W-14, in order to purify their The most active isolated fraction, which was determined based on the bioassay of Example 13, was established. Typically, 4-20 L of filtered broth (as described in Example 13) was obtained and concentrated using an Amicon spiral ultrafiltration cartridge S1Y100 connected to an Amicon M-12 filter unit. The retentate contains natural proteins composed of molecules with a size greater than 100 kDa, but the flow-through material contains natural proteins less than 100 kDa. Most of the anti-SCR activity is present in the 100 kDa retentate. The retentate was then continuously diafiltered with 10 mM sodium phosphate (pH = 7.0) until the filtrate reached A28Q < 0.01. Unless otherwise stated, the buffers for all subsequent procedures are defined by 1 OmM sodium phosphate (pH 7.0). The retentate was then concentrated to a final volume of about 0.20 L and filtered through a 0.45 mm NalgeneTM sterile filtration unit. The filtered material was packed into a Pharmacia HR16 / 10 column at 7.5 ml / min. It was packed with a PerSeptive Biosystem Poros® 50 HQ strong anion exchange matrix equilibrated in buffer using a PerSeptive Biosystem Sprint® HPLC system. After packing, the column was washed with buffer until A280 < 0.01 was reached. The protein was then eluted from the column at 2.5 ml / min using a buffer containing 0.4 M NaCl for 20 minutes, with a total buffer volume of 50 ml. The column was then washed with a buffer containing 1.0 M NaCl at the same flow rate for another 20 minutes (final volume 50 ml). The proteins eluted with 0 · 4M and 1.0M NaCl are placed in a separation dialysis bag (Spectra / Por® -122- This paper size applies to the Chinese National Standard (CNS) A4 specification (210X2W mm) ----- ---- 0-pack-(t read the notes on the back before filling this page), order 509722 printed by the Consumer Cooperatives of the Central Standards Bureau, Ministry of Economic Affairs, printed A7 B7 V. Description of the invention (120) '^ Membrane MWC0: 2 , 000) and dialyzed overnight at 40C in 12 L of buffer solution ^ Most of the anti-SCR activity is present in the 0.4M separation. The 0 4m fraction was further purified at a flow rate of 0.75 ml / min by adding 20 ml to a-σ a king Pharmacia χκ 26/100 column packed with Sepharose CL4B (Pharmacia). The separations were pooled based on the spike images and concentrated to a final volume of 0.75 ml using a Millipore Ultrafree®-15 centrifugation filter equipped with Biomax-50K NMWL membrane. The protein stomach concentration was determined by using the Biorad protein analysis kit and bovine gamma globulin ^ # standard. Characteristic The natural molecular weight imparted to the SCR toxin complex was measured using a pre-filled Pharmacia HR 16/5, Sepharose CL4B in buffer. The column is then calibrated with a protein of known molecular size to make the toxin approximate the natural molecular size. As shown in Table 21, the molecular size of this toxin complex ranges from 777 kDa of the Hb strain to 1,900 kDa of the WX-14 strain. The production of toxin complexes also varies, from 0.8 mg / L to 7.0 mg / L of Hb strain produced by WX-12 strain. The protein found in the toxin complex was analyzed by SDS-PAGE to determine the size of individual peptides. Typically, 20 mg of the toxin complex protein from each strain was packed into a 2-15% polyamidamine gel (Integrated Separation System) and electrophoresed at 20 mA in Biorad SDS-PAGE buffer. After the electrophoresis, the gels were stained overnight in Biorad bitumin blue R-250 (0.2% dissolved in methanol: acetic acid: water; 40: 10: 40 v / v / v). Then, the gel was decolorized in methanol: acetic acid: water; 40 ·· 10 ·· 40 (v / v / v). The _ -123-This paper size applies to China ^ & quasi (CNS) A4 specification (210X297 mm) " C Please read the notes on the back before filling in this page} • Binding · Order 4 · 509722 A7 ____B7 5 Description of the invention (ii) The gels were then washed with water for 15 minutes and scanned with a Molecular Dynamics Personal Laser Densitometer®. Quantitative and molecular size calculations for each column are compared to Biorad high molecular weight standards, which range from 200 to 45 kDa. The individual sizes of the peptides contained in the SCR toxin from each strain are listed in Table 22. Individual peptides range in size from 230 kDa in the WX-1 strain to 16 kDa seen in the WX-7 strain. Each strain (except the Hb strain) has multiple peptides ranging from 160-230 kDa, 100-160 kDa, and 50-80 kDa contained in the toxin complex. These data show that the composition of the toxin complex between the peptide composition and the strain may be different, however, the properties of the toxin appear to be a large oligomeric protein complex in all cases. (Please read the notes on the back of the 4 items before filling in '' Pack — write this page)

1T printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs. 21 Special properties of toxin complexes derived from non-W-14 coryneform strains. Gives the strains approximate natural molecular weight. A Production of active isolated fraction (mg / ml) b. H9 972,000 1.8 Hb 777,000 7.0 Hm 1,400,000 1. 1 HP88 813,000 2. 5 NCI 1,092, 000 3.3 WIR 979,000 1. 0 WX-l 973,000 0.8 WX-2 951,000 2.2 WX-7 1,000,000 1.5 WX -12 898,000 0.4 WX -14 1,900,000 1.9 W -14 860 · 000 7.5 a Natural molecular weight was determined using a Pharmacia HR 16/50 column packed with Sepharose CL4B. b Amount of toxin complex recovered from the culture broth. • 124- This paper size is in accordance with Chinese National Standard (CNS) A4 specification (210X297mm) • Continued 509722 A7 B7 V. Description of the invention (122) Activity Spectrum As shown in Table 23, the purification is from Hm and H9 The toxin complexes of the strains were tested for their activity against different insects, and the toxin complexes from the W-14 strain were compared. The analyses were performed as described in Example 13. The toxin complex from all three strains showed activity against tobacco budworms, European corn perforators, southern corn rootworms, and spodoptera. Furthermore, the toxin complex from Hm and W-14 strains showed activity against the two-spot spider tapeworm. In addition, the toxin complex from W-14 showed activity against mosquito larvae. These data show that although the toxin complex has similar activity in some orders of insects, it can also show different activities against other insect orders. Table 22 Approximation of purified toxin complexes derived from non-W-14-derived fungi (t read the precautions on the back of A before filling out this page) -1190180170160150130120110939077696563605146403929 4 WX-1210180160120110100958069494133 2ffx-l180160140139130110s £ 5s? 2sgsws ^ s WX-7200180110877543332826232221191816 WX-2I170150120110SS old do X-12301901701601098765853413531282422 ff 1 WIR1701601201108979746251403937333028272523 NC-1801701401104416 HP881701601401301291101003 K ^ ^ r010) 0ur28960209 Hm171410876443221 0090008815907494975 b5433209887665544333 Η 1 * 11 11 lx lx 11 00000874928072607 987642988776655443 Η 1 1 1 1 1 -125- This paper size applies the Chinese National Standard (CNS) A4 specification (210X297 mm) 509722 A7 B7 V. Description of the invention (M) M3_ 被 _Observed by light defense Insecticidal of the inactivated coil complex of the fungus bacillary fungus Corynebacterium strain Hm toxin complex H9 toxin complex iy_i4 poison I complex _ * mortality or growth inhibition greater than 25% ** 1 ·· Tobacco Insects; 2: European corn borer; 3: southern corn rootworm; 4: mosquitoes; 5: two-spotted spider mite composition; 6: Star shaped springtails, 7. Drosophila; 8: the cotton boll weevil. Sensitive * insect species 1 ^, 2,3,5,6,7,8 1,2,3,6,7,8 1,2,3,4,5 6. 7. Central Bureau of Standards, Ministry of Economic Affairs Printed by Employee Consumption Cooperative Example 15 Secondary Isolation of Corynebacterium Protein Toxin Complex The Corynebacterium protein toxin complex was isolated as described in Example 14. Next, approximately 10 mg of toxin was applied at a flow rate of 1 ml / min to a MonoQ 5/5 column equilibrated with 20 mM Tris-HCl (pH 7.0). The column was washed with 20 mM Tris-HCl (ρΗ7.0) until the optical density at 280 nm returned to the baseline absorbance. The proteins bound to the column were eluted with a linear gradient of 0 to 梯度 · Μ NaCl in 20 mM Tris-HC1 (ρ17.0) at 1 ml / min for 30 minutes. A 1 ml fraction was collected and subjected to Southern Corn Root Worm (SCR) bioanalysis (Example 13). The spike in activity in the SCR bioassay was determined by serial dilution of each fraction. Two active spikes against SCR were revealed and referred to as A (eluted at about 0.2-2. 3M NaCl) and B (eluted at about 0.33 NaM). Active spikes a and B were pooled separately, and 2 and # were further purified using one of the three-step procedures described below. A solid (NHOgSO4 was added to the above-mentioned protein separation portion to a final concentration. The protein was then added to a Superose 5/5 column at 1 ml / min (with (NΗ4) βο4 in 50 mM potassium phosphate buffer). Equilibrium). Le7i 1.7¾ for these proteins bound to the column (please read the notes on the back before filling out this page) Binding · Binding-126- This paper is suitable for money_ (CNsT ^ i: 格 （21〇x297 Γ 509722 A7 B7 V. Description of the invention (124) (NH4) 2S04, 0% ethylene glycol, 50 mM potassium phosphate (ρΗ7 · 0) to 25% ethylene terminal '25 mM potassium phosphate (PΗ7 · 0, excluding ( The linear gradient of NH4) 2S04) was eluted at 0.5 ml / min. The fractions were dialyzed overnight with potassium phosphate buffer (PH7.0). The anti-activity in each fraction was determined by biological analysis. The separated fractions were pooled and added to a MonoQ 5/5 column equilibrated with 20 mM Tris-HCl (pH 7.0) at 1 ml / min. The proteins bound to the column were borrowed by a 20 mM Tris-HC1 (ρΗ7 · 0 ) In a linear gradient of 0 to 1.0M NaCl eluting at 1 ml / min. In the final step of purification, the aforementioned most active separation fraction Bioanalytical assays) were pooled and applied to a monophenyl-SuPerose 5/5 column. Solid (NH4) 2S04 was added to a final concentration of 1.7 M. The solution was then added to the 50 mM solution at 1 ml / min. 1.7M (NH4) 2S04 equilibrated column in potassium phosphate buffer solution. The proteins bound to the column were decompressed with 1 · 7M (NH4) 2S04, 50 mM acid filling (ρΗ7 · 0) to 25 mM structure. The linear gradient of acid unloading (ρΗ7 · 0) was eluted at 0.5 ml / min. The fractions were dialyzed overnight with 10 mM potassium phosphate buffer (pH 7.0). The anti-SCR activity in each fraction was determined by biological analysis. . I printed by the Consumers' Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs-0 · C, please read the notes on the back before filling out this page) ί% The above-mentioned 3-step procedure The final purified protein from A peak is called A toxin The final purified protein from the B spike is called B toxin. The characteristics of A toxin and B toxin are conferred and amine is determined. In SDS-PAGE, both toxin A and toxin B contain 2 major peptides (higher than 90% of all bitter Maki-stained proteins): 192kDa (referred to as A1 and B1, respectively) and 58kDa (referred to as A, respectively) 2 and B2). A toxin and B toxin two-127-This paper size is applicable to the Chinese National Standard (CNS) A4 specification (210 × 297 Gongchu) 509722 A7 __. _B7 V. Description of the invention (125) on the natural PAGE Only one major band appears, showing that A1 and A2 are the subunits of a protein complex, and B1 and B2 are the subunits of a protein complex. The natural molecular weights of both A toxin and B toxin were determined to be 860 kDa by gel filtration chromatography. The relative molar concentration of A1 to A2 was determined to be 1: 1 by density analysis of SDS-PAGE gel. Similarly, Bl and B2 peptides are present at the same molar concentrations. A toxin and B toxin were electrophoresed on 10% SDS-PAGE and transduced to the PVDF membrane. The transcripts were sent to Harvard MicroChem and Cambridge ProChem for amino acid analysis and N-terminal amino acid sequencing, respectively. The N-terminal amino acid sequence of B1 was determined to be the same as the sequence identification number 1 and the TcbAu region of the icW gene (sequence identification number 12, positions 87 to 99). A unique N-terminal sequence was obtained from the B2 peptide (sequence identification number 40). The N-terminal amino acid sequence of the B2 peptide is the same as the TcbAUi region (sequence identification number 12, positions 1935 to 1945) of the amino acid sequence derived from the gene. Therefore, the b toxin Mainly contains TcbAH and

Two peptides of TcbAiu, which were observed to be derived from the same gene product, TcbA. Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (please read the notes on the back before filling this page) The N-terminal sequence of A2 (Serial Identification Number 41) is unique compared to this TcbAiu peptide and other peptides . This A2 peptide was named TcdAHi (see Example 17). Sequence identification number 6 was determined to be a mixture of amino acid sequence identification number 40 and sequence identification number 41. The A1 and A2 peptides were further subjected to internal amino acid sequencing. For internal amino acid sequencing, 10 pg of A toxin was electrophoresed in 10% SDS-PAGE and transferred to a PVDF membrane. After the transfection product was dyed with sulfonamide black, fluorene and fluorene 2 peptides (respectively ____—____ -128-this paper size applies the Chinese Standard (CNS) 8 4 ^^ 21〇 > < 297 public celebration) 509722 Printed by the Consumer Standards Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs A7 ___B7 V. Description of the Invention (3) Named TcdAn and TcdAiH) Cut from the turnaround and sent to Harvard MicroChem and Cambridge ProChem. The peptides were digested with pepsin, and the individual peptides were separated by HPLC chromatography. N-terminal amino acid sequence analysis was performed on the selected pepsin-digested peptide fragments. The internal amino acid sequence of two segments of the A1 peptide (TcdAu-PK71, sequence identification number 38 and TcdAu-PK44, sequence identification number 39) was found and the deduced amino acid sequence of the TcbAn region of the tcbA gene (Serial Identification Number 12) has significant homogeneity. Similarly, the N-terminal sequence (sequence identification number 41) and the two internal sequences of the A2 peptide (T ^ (1ΑίΗ-ΡΚ57, sequence identification number 42 and TcdAiH-PK20, sequence identification number 43) ) The amino acid sequence (sequence identification number 12) deduced from the TcbAiu region of the icW gene also showed significant homogeneity. To summarize the foregoing results, the toxin complex contains at least two active protein toxins against SCR The complex, namely A toxin and B toxin. A toxin and B toxin are similar in nature and subunit molecular weight, but their peptide composition is different. A toxin contains TcdAn and TcdAiU peptides as the main peptide and B The toxin contains TcbAu and TcbAiu peptides as the main peptides. Toxin C, Tea-like purified peptides are isolated. The Bacillus protein toxin complex is isolated as described above. Secondly, about 50 mg of toxin is used at a flow rate of 2 ml / min. A MonoQ 10/10 column equilibrated with 20 mM Tris-HCl (pH 7 · 0) was added. The column was washed with 20 mM Tris-HCl (ρΗ7 · 0) until the optical density at 280 nm returned to the baseline level. Binding To the column Elution was performed with a linear gradient of 0 to 1.0M NaCl in 20 mM Tris-HC1 (ρΗ7.0) at 2 ml / min for a duration of -129-. This paper standard is applicable to the Chinese National Standard (CNS) A4 specification (21〇X: 297 mm) (Please read the note on the back 4 ▼ and fill in k — — write this page), 11 4 509722 Printed by A7 __; __B7 __ of the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 5. Description of the invention (127 ) 60 minutes. 2 ml fractions were collected and analyzed using pAb TcaBn-syn antibody (see Example 21) as a primary antibody for Western analysis. The fractions reacted with pAb TcaBH-syn antibody were combined and added (NH4) 2S04 to 1 · The final concentration of 7M. The protein was then added to a phenyl-Superose 10/10 column equilibrated with 1. 7M (NH4) 2S04 in 50 mM potassium phosphate buffer (ρΗ7.0) at 1 ml / min. Binding to The column protein was eluted with a linear gradient of 1.7M (NH4) 2S04, 50 mM potassium phosphate (pH 7.0) to 10 mM potassium phosphate (ρΗ7 · 0) at 1 ml / min for 120 minutes. The 2 ml fraction was collected, Dialyzed overnight with 10 mM potassium phosphate buffer (pH 7.0) and used pAb TcaBn-syn antibody as primary Antibodies were analyzed by Western transfection. Separated fractions cross-reacting with the antibodies were pooled and applied to a MonoQ 5/5 column equilibrated with 20 mM Tris-HCl (pH 7.0) at a flow rate of 1 ml / min. The proteins bound to the column were eluted with a linear gradient of 0 to 1.0M NaCl in 20 mM Tris-HCl (ρΗ7 · 0) at 1 ml / min for 30 minutes. The aforementioned separated fractions that reacted with the pAb TcaBu-syn antibody were pooled and applied to a monophenyl-8H 61: 036 5/5 column. Add solids (Servo 4) to a final concentration of 2804 to 1 ·]. The solution was then added to a column equilibrated with 1.7 M (NH4) 2S04 in 50 mM potassium buffered saline (pH 7.0) at 1 ml / min. The protein bound to the column was then washed with a linear gradient of 1.7M (NH4) 2S04'50mM sulfonate (ρΗ7.0 ·) to 10mM potassium phosphate (pH7.0) at 0.5ml / min. Elution lasted 60 minutes. The separated fractions were dialyzed overnight with 10% potassium-buffered saline (pH 7.0 ·). In the final purification step, the separated fractions reacted with pAb TcaBu-syn antibody determined by Western analysis as described above were combined, and were -130 at lml / min. ___ This paper is in accordance with China National Standard (CNS) A4 (21 OX297) (Mm) (Please read the notes on the back before filling out this page) • Binding, binding, and clocking 509722 A7 B7 Printed by the Employees ’Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs 5. Description of the invention (128) Add a 20mM Tris-HCl ( pH 7.0) Equilibrated Mono Q 5/5 column. The proteins bound to the column were eluted with a linear gradient of 0 to 1.0M NaCl in 20 mM Tris-HCl (ρΗ7.0) at lmi / min for 30 minutes. The final purified protein fraction contains 6 major peptides determined by SDS-PAGE: 165kDa, 90kDa, 64kDa, 62kDa, 58kDa, and 22kDa. The LD50 of the insecticidal activity of this purified isolated fraction was determined to be 100 ng (against SCR) and 500 ng (against ECB). The aforementioned peptides were transferred to a PCDF membrane, and the disrupted products were sent to Harvard MicroChem and Cambridge ProChem for amino acid analysis and N-terminal sequencing of five amino acids, respectively. The N-terminal amino acid sequence of the 165 kDa peptide was determined to be the same as the TcaC peptide (sequence identification number 2, position 1 to 5). The N-terminal amino acid sequence of the 90 kDa peptide was determined as the TcaAii region of the derived amino acid sequence of the icad gene (sequence identification number 33, positions 254 to 258). The N-terminal amino acid sequence of the 64 kDa peptide was determined to be the same as the τ c a Bi peptide (sequence identification number 3, positions 1 to 5). The N-terminal amino acid sequence of the 62 kDa peptide was determined as the TcaAH region (sequence identification number 33, 489 to 493 positions) of the derived amino acid sequence of the ICaj gene. The N-terminal amino acid sequence of the 58 kDa peptide was determined to be the same as the TcaBH peptide (sequence identification number 5, position 1 to 5). The N-terminal amino acid sequence of the 22kDa peptide was determined as the TcaAii region (named TcaAiv, sequence identification number 34, 98th to 102th positions) of the derived amino acid sequence of the icaJ gene. It should be noted that all the icaJ, ica and icaf genes exist in the same ica operator (Figure 6A). -131- (Please read the note on the back 4-item before filling. Pack-: write this page)

、 1T This paper size applies to Chinese National Standard (CNS) A4 (210X297 mm) Printed by the Consumer Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs 509722 A7 B7 V. Description of the invention (129) 5pg purified Tea separation part, purified A toxin The following antibodies were used as the primary antibodies and purified B toxin, respectively, and analyzed by Western transfusion: pAb TcaBH-syn antibody, mAb CF52 antibody, pAb TedAii-syn antibody and pAb Tedni-syn antibody (Example 21). Only the purified Tea peptide isolated portion reacted with the pAb TcaBu-syn antibody, but not A toxin or B toxin. Only B toxin reacted with mAb CF52 antibody, but Tea peptide isolated part or A toxin did not. Only the A toxin reacted with the pAb TedAii-syn antibody or the pAb Tediii-syn antibody, but the Tea peptide isolated portion or the B toxin did not. This indicates that the insecticidal activity exhibited in the purified Tea peptide isolated portion is independent of A toxin and B toxin. The purified Tea peptide isolated part is the third unique protein toxin, which is named C toxin. Example 16 Cleavage and activation of TebA peptides In the B toxin complex, TcbAH and TcbAUi peptides were derived from the single gene product TebA (Example 15). This treatment of TebA peptides to TcbAH and TcbAHi is presumably due to the action of the bacillus protease and the metal protease described in Example 10 (which is most likely). In some cases, it should be noted that when the Corynebacterium w-14 broth is processed, the TebA peptide is present in the B toxin complex as a major component other than TcbAu and TcbAUi peptides. The same procedure (purification of the B toxin complex as described in Example 15) was used to separate the toxin complex from W-14 broth to increase the TebA peptide. The final purified material was analyzed in a 4-20% gradient SDS-PAGE, and the main peptide was quantified by a densitometer. TebA, TcbAu and TcbAin were determined to contain 58%, 36% and 6% of the total protein, respectively. The equivalence of these peptides is based on SDS-PAGE and their individual molecules in western analysis using monospecific antibodies. -132- This paper is sized to the Chinese National Standard (CNS) A4 (210X297 mm)- -------- ^-Batch clothing-(Please read the precautions on the back first-SV fill in this page)

1T 509722 A7 B7 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 5. The invention description (130) is small and confirmed. The natural molecular weight of this fraction was determined to be 860 kDa. TcbA cleavage was evaluated by treating the purified substance with purified 38kDa and 58kDa W-14 coryneform metalloproteinases (Example 10). Pepsin (Sigma, MO) was the control enzyme. The standard reaction consisted of 17.5 pg of the purified fraction described above, 1.5 units of protease, and 0.1 M Tris buffer (ρΗ8.0) in a total volume of 100 μ1. In this control reaction, the protease was omitted. The reaction mixtures were incubated at 37QC for 90 minutes. At the end of the reaction, take 20 µl and immediately boil with SDS-PAGE sample buffer for electrophoretic analysis with a 4-20% gradient SDS-PAGE. As determined by SDS-PAGE, the amount of TcbAn and TcbAiU peptides increased by about 3 times in 38kDa and 58kDa protease treatments, while the amount of TcbA wins decreased proportionally (Table 24). This relative decrease and the increase in selected peptides were confirmed by Western turnaround analysis. Furthermore, gel filtration of the cut material shows that the natural molecular size of the complex remains the same. In the treatment of pepsin, TcbA and TcbAu peptides are non-specifically digested into small peptides. This represents that 38 kDa and 58 kDa bacillus proteases can specifically treat TcbA peptides into peptides. The protease processor and the untreated control group of the remaining 80 µ1 of the reaction mixture were serially diluted with 10 mM sodium phosphate buffer (ρΗ7.0) and analyzed by SCR biological analysis. By comparing the activity of many dilutions, the 38 kDa protease treatment was determined to increase the SCR insecticidal activity by about 3 to 4 times. The growth inhibition of the remaining insects during the protease treatment was also more severe than that of the control group (Table 24). Table 1-4_ TcbA peptides treated with protein have been transformed into human ^ and TcbA ^ peptides. 133- This paper size applies to China (CNS) A4 specifications (210X297 mm) (Please read the note on the back first) Please fill in this page for more details)

1T i0 509722 Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs A7 B7 V. Description of the invention (131) Control group 38kDa protease treated TcbA (% of total protein) 58 18 TcbAu (% of total protein) 36 64 TcbA & · ( 90 6 18 LD50 (gg protein) SCR weight (mg / insect) * 0 · 0.52 0 · 1 * ··· Growth inhibition index of the average weight of surviving insects after 5 days of ingestion in the analysis. In the second proof of proteolytic activation, SCR intestinal peptone activation test is used to test whether W-14 toxin is processed by insects. For example, by using SDS-PAGE of single-source antibody and Western analysis It was determined that the toxin B purified from Corynebacterium w-14 broth (Example 15) mainly contained intact TcbA peptides. The LD50 against SCR of this isolated part was determined to be about 700 ng. SCR larvae grew on coleoptera feed To the 4th instar larva (approximately 100-125mg total weight per insect). The contents of the SCR intestines were collected as follows: the intestines were removed using scalpels and tweezers. After removing the excess fatty matter wrapped in the intestinal lining, 40 segments of intestine One ΙΟΟμΙ sterile water was homogenized micro centrifuge tube. The tube is then at 14, OOOxg centrifugation for 10 min and the precipitate was discarded. The supernatant is stored before use in a refrigeration cabinet -70〇C.

The B toxin was evaluated by treating the intestinal tract of the insect with the collected SCR intestinal contents by treating the aforementioned purified B toxin. The reaction consisted of 40 pg beta toxin (1 mg / ml), 50 μl of SCR intestinal content, and 0.1 M Tris buffer (pH 8.0) in a total volume of 100 μl. In this control group reaction, the contents of the SCR bowel were omitted. The reaction mixture was incubated overnight at 37GC. At the end of the reaction, take 10μ1 and boil it with an equal volume of 2x SDS-PAGE sample buffer for SDS-PAGE -134. The paper size applies the Chinese National Standard (CNS) A4 specification (210 × 297 mm) I ---- --- 4 Equipment— (Please read the precautions on the back before filling this page), -ιτ • suspect · 509722 A7 _____B7 V. Description of the invention (132) Analysis. The remaining 90 μl of the reaction mixture was serially diluted with 10 mM sodium phosphate buffer (ρ 液 7.0) and analyzed by SCR bioanalysis. SDS-PAGE analysis showed that the TcbA peptide was completely digested into smaller peptides during SCR intestinal contents treatment. Analysis of the non-denatured toxin isolated portion showed that the native size (about 86 kDa) remained the same even if the larger peptide was fragmented. In the SCR bioassay, the LD50 of SCR intestinal-treated b-toxin was found to be about 70 ng, representing a 10-fold increase. In another experiment, the protease] (treatment completely removed toxin activity. Example 17 Screened the gene library encoding the gene to encode the TcdAn peptide (described as sequence identification number 17, namely TcdAu-PTlll internal peptide N -End sequence and sequence identification number 18, that is, the gene selection and characterization of one of the genes of TCdAH-PT79 peptide N-terminal sequence) have been completed. It is designed to encode the sequence identification number 17 (Table 25) and Two groups of degenerate oligonucleotides of the amino acid sequence of sequence identification number 18 (Table 26) and their reverse complements of their sequences were synthesized as in Example 8. The DNA sequences of these oligonucleotides are shown below ·· -------- 10 ^! -----, 玎 ----- (t read the precautions on the back before filling out this page) Printed by the Staff Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs • 135 -This paper size applies to China National Standard (CNS) A4 (210X297 male f) 509722 A7 B7 V. Description of invention (133) Table 2 5 Degenerate oligonucleotide P2-PT111 of sequence identification number 17 1 2 3 4 5 6 7 8 Ala Fhe Asa lie Asp Asp Val Ser Dense Phosphor 5, GCN TT (T / C) AACT / C) ATCT / C / A) GA (T / C) GACT / C) GTN 3, P2.3.6. CB 5, GC (A / C / G / T) TT (T / C) AAT ΚΠ GAT GAT GT 3, P2.3.5 5, GC (A / C / G / T) TT (T / C) AA (T / C) ATCT / C / A) GACT / C) GA (T / C) GT 3, P2.3.5S 5, AC (G / A) TC (G / A) TC (T / G / A) AT (G / A) H (G / A) AA (A / C / G / T) GC 3, P2.3.5RI 5'ACI TCI TCI ATI TTI AAI GC 3 , P2.3R.CB 5, CAG (A / G) CT (A / C) AC ATC ATC AAT ΑΠ AAA 3, (Please read the notes on the back before filling this page) Table 26 Preface 1 丨 Identification Number 1 Degenerate Oligonucleotide of Π8, P2-PΓ79 1 2 3 4 5 6 7 8 9 10 11 12 13 Fhe lie Tal Tyr Thr Ser Leu Gly Tal Asa Pro Asa Asa * Phosphorus and 5ΊΤΥ ATH GTN TAY ΑΟί 6 6 GGN GTN AAY CCN AAY AAY 3, P2.79.2 5ΤΓΥ ATY GTK TAT ACT TCI YTR GGY GTK AAT CCR AAT AAT 3, P2.79.3 5, TTT ΑΠ GTK TAT ACY AGY YTR GGY GTK AAT CCR AAT AAT 3, P2.79.R .1 5, Απ ΑΠ YGG AH MAC RCC YAR RCT RGT ATA MAC AAT AAA 3, P2.79R.CB 5, Απ Απ YGG m MAC ACC CAG RCT GGT ATA MAC AAT AAA 3, * Based on the IUPAC-IUB nucleotide code , Y is C or T, Η is A, C or T, N is A, C, G or T, K is G or T, R is A Or G and M are A or 0. The polymerase chain reaction (PCR) printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs is essentially implemented as described in Example 8, which uses P2 in all forward / reverse combinations. 3. 6. CB or P2.3.5 are forward primers, P2.79.R.1 or P2.79R.CB are reverse primers, and the DNA of Bacillus spp. W-14 gene is used as a template. In another set of reactions, P2. 79.2. Or P2. 79.3 was used as a forward primer and P2. 3.5R. 1 or P2. 3R. 5RI was used as a reverse primer in all the forward / reverse combinations. Only if P2. 3. 6. CB is used as the forward primer and P2.79.R. 1 or P2. 79R. CB is used as the reverse primer -136-This paper size applies the Chinese National Standard (CNS) A4 specification (210X 297 mm) 509722 A7 B7 Printed by the Consumers' Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs 5. Description of the Invention (134) In the reaction, a non-human amplification product can be seen, which is the estimated size of 2500 base pairs (in agarose gel) The movement of glue). The sequence of the primers used to obtain this amplification product showed that the TcdAH-PT111 peptide fragment was located on the amine side of the TcdAH-PT79 peptide fragment. The 2500bp PCR product was ligated to a pCRTMI I plastid vector (Invitrogen, San Diego, CA) according to the supplier's instructions, and the DNA sequences spanning the ends of the embedded fragments of 2 isolates (HS24 and HS27) This is determined using the primers recommended by these vendors and the sequencing methods described previously. The sequences were identical for the two isolates. The new primer was based on the determined sequence and was used to prime other sequencing reactions to obtain all 2,557 bases of the insert (sequence identification number 36). By translating the peptide of this part encoded by sequence identification number 36, it was revealed that the sequence is sequence identification number 37 of the 845 amino acids. Analysis of the protein homogeneity of this portion of the TcdAH peptide fragment showed comparable amino acid homogeneity to residues 542 to 1390 of the TcbA protein (68% similarity and 53% identity; using the Wisconsin Package 8th edition, Genetic Computer Group (GCG), Madison, WI), or equivalent amino acid homology for residues 567 to 1389 (60% similarity and 54% identity; using the Wisconsin Package Version 9.0, Genetic Computer Group (GCG), Madison, WI). Therefore, the sequence identification number No. 36 represents that the gene obviously produces a protein similar to but different from the TcbA protein, which may have biological activities similar to but different from the TcbA protein. In another example, one encodes the TcdAH-PK44 peptide and TcdAiU 58kDa -137 · (please read the notes on the back _ J · > then fill in-pack-: write this page),? Τ 4 papers The scale is applicable to the Chinese National Standard (CNS) A4 specification (210X29? Mm) 509722 A7 B7 Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs 5. Description of the invention (I35) N-terminal peptide (described as the sequence identification number 39 The peptide sequence of TcdAu-PK44 and sequence identification number 41, Tε (1ΑΗί 58kDa N-terminal peptide sequence) were isolated. It was designed to encode sequence identification number 39 (Table 28) and sequence identification. Two groups of degenerate oligonucleotides of the amino acid sequence numbered 41 (Table 27) and their reverse complements of their sequences were synthesized as in Example 8. Table 2 7 Degenerate oligos of sequence identification number 41 Glycoside dense phosphorus subscript% 1 > 3 4 £ 6 ； 1 ^ i 1 £ 1 10 11 12 13 14 Amino acid I • eu i Irg S ier l Lla i Lsn 1 rhr i «eu 1 rhr / Lsp Leu Phe Leu Pro Gin A2.1 5 'YTR (: GY h lGY g; CI / lAT / lCY \ rTR / lCY C; AT YTR TTT YTR CCR CA 3f Α2 · 2 A2.3.R c; CI / lAT A iCI \ rTR h lCT C; AY YTR TTY YTR IGC CCT CA 3 'ci, TG \ rGG \ rAR / lAA \ rAR E [TC E! GT \ rAR RGT RTT RCT RCG RCG 3 A2.4.R gi, TG 1: GG C: AG / LAA C: AG F [TC IGT CAG IGT ATT IGC 3 袅 28 Degenerate Oligonucleotide Sequence Number No. 39% (8 ) (9) (10) (11) (12) (13) (14) (15) (16) Codon number 1 2 3 4 5 6 7 8 9 `` Gly Pro Val Glu lie Asn Thr Ala lie Α1 · 44 · 1 A1.44.1R 5, GGY CCR GTK GAA ATT AAT ACC p GCI AT 3 '5, ATI GCG GTA TTA ATT TCM ACY GGR CC 3, Α1.44.2 5, GGI CCI GTI GAR ATY AAY ACI GCI AT 3 'A1.44.2R 5, ATI GCI GTR TTR ATY TCI ACI GGI CC 3, polymerase chain reaction (PCR) is essentially implemented as described in Example 8, which uses A1 · 44 in all forward / reverse combinations. 1 or A1 · 44. 2 forward primers and A2.3.R. or A2.4R reverse primers, using the DNA of Corynebacterium w-14 gene as a template. In another set of reactions, use A2 · 1 or A2.2 as forward primers and A1.44.1R or A1.442R as -138- in all forward / reverse combinations (Please read the note on the back J0 first Refilling and loading of items-: Write this page) The paper size is applicable to China National Standard (CNS) A4 (210 X 297 mm)

1T .0 509722 A7 B7 V. Description of the invention (l36) Reverse primer. Only in the reaction containing A. 44. 1 or A1. 44. 2 as the forward primer and A2.3R as the reverse primer, a non-artificial amplification product can be seen, which is the estimated size of 1400 base pairs (in Mobility of agarose gel). The sequence of the primers used to obtain this amplification product showed that the TcdAu-PK44 peptide fragment was located on the amine side of the 58 kDa peptide fragment of TcdAiH. The 1400bp PCR product was ligated to the pCRTMI I plastid vector (Invitrogen, San Diego, CA) according to the supplier's instructions. The DNA sequences spanning the ends of the embedded fragment of the 4 isolates were determined using primers recommended by the suppliers and the sequencing methods described previously. The nucleic acid sequences of all isolates are expected to be different in the regions corresponding to the degenerate primer sequences, but the amino acid sequences deduced from this information and the peptides previously determined The actual amino acid sequences (the sequence identification numbers 41 and 39) are the same. The Consumer Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs printed the W-14 gene condensate gene bank as described in Example 8 using a radioactively labeled probe containing a previously prepared DNA (sequence identification number 36). Five hybrid condensate isolates were identified by screening and named 17D9, 20B10, 21D2, 27B10, and 26D1. These aggregates are different from those previously identified by probes corresponding to the genes described as sequence identification numbers 11 or 25. Restriction enzyme analysis and DNA fragmentation hybridization identified 3 and / or fragments (approximately 3 · 7, 3 · 7, and 1 · lkbp), which spanned the region containing the sequence identification number 36 DNA. Using the radioactively labeled 1.4 kbp DNA fragment prepared in this example as a probe, screening of the W-14 genomic condensate gene library identified the same 5 > (Head-139- This paper size applies China National Standard (CNS) A4 specification (210X297 mm) Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs 509722 A7 B7 V. Description of the invention (137) Condensates (17D9, 20B10, 21D2, 27B10 and 26D1). And ^ / Digestive condensate DNA hybridization of DNA disruption hybridization also shows that it can hybridize to a subset of the same value M / fragment (as seen with the 2.5kbp TcdAii gene probe) 'It means that both fragments The DNA sequence of the genomic DNA encoding 0 valence M / fragment of this colony determines the uninterrupted reading structure of a 7751 salt-based pair (sequence identification number 46), which encodes a 2516 amine group 282. 9kDa protein (SEQ ID No. 47). Analysis of the amino acid sequence of this protein revealed all expected TcdAH peptides (SEQ ID Nos. Π, 18, 37, 38, and 39) and TcdAni Peptide N-terminus (sequence ID number 41), and all TcdAHi internal peptides (sequence identification numbers 42 and 43). The peptides that were separated and identified as TcdAn and TcdAii are the open read architecture (named as ic, the disclosure description is each product of the sequence identification number 46). Furthermore, the sequence identification number 47 is displayed, starting at the 89th position and disclosed and explained as the sequence identification number 13 (which is The N-terminal sequence of a peptide (approximately 201 kDa in size) means that the original protein generated from sequence identification number 46 was processed in a similar manner as previously disclosed for sequence identification number 12. In addition, the protein was Further cleavage to produce a product with a size of 209.2 kDa, which is encoded by sequence identification number 48 and disclosed as a sequence identification number 49 (TcdAH peptide); and a product with a size of 63.6 kDa, which Encoded by sequence identification number 50 and disclosed as sequence identification number 51 (TcdAiu peptide). Therefore, this -140- paper size confirmed to be A toxin (Example 15) Use Chinese National Standard (CNS) M specifications (210X297 mm) -------- Install-Λ J (Please read the precautions on the back before filling this page) Order 509722 Α7 Β7 V. Description of the invention (138 ) The insecticidal activity is thought to be derived from these products of sequence identification number 46, which is processed by the disclosure as the sequence identification number 47 of the 282 · 9kDa full-length protein to be processed to generate the disclosure description as the sequence identification number The peptides Nos. 49 and 51 are exemplified. The insecticidal activity confirmed to be B toxin (Example 15) is thought to be derived from these products of sequence identification number 11 which is exemplified by the 280.6 kDa protein identified by sequence identification number 12 By. This protein was subjected to proteolytic processing to produce the 207.6 kDa peptide whose disclosure description is sequence identification number 53 (which is encoded by the sequence identification number 52) and N which has the disclosure description as sequence identification number 40. The 62.9 kDa peptide of the -terminal sequence (the N-terminal sequence is further disclosed as sequence identification number 55, which is encoded by sequence identification number 54). These amino acid sequence comparisons between proteins identified by sequence identification number 12 and sequence identification number 47 show that using the Wisconsin Package version 8.0 'Genetic Computer Grooup (GCG), Madison, WI has 69% Similarity is 54% identical, or version 9.0 using the program has 60% similarity and 54% similarity. The high degree of this evolutionary relationship is not uniform among the entire amino acid sequences of these peptides, but is higher at the carboxy terminus of the proteins; because the disclosure description is sequence identification number 51 (derived from the sequence Identification number 47) and the peptides disclosed as sequence identification number 55 (derived from sequence identification number 12) utilize the Wisconsin Package version 8.0, Genetic Computer Grooup (GCG), Madison, WI has 76% similarity and 64% similarity, or 71% similarity with version 9.0 of the program -141-This paper size applies the Chinese National Standard (CNS) A4 specification (210 × 297 mm) ---- -· Equipment-Please read the notes on the back of the page and fill in this page.) Ordered by the Consumer Standards Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs and printed on 509722 A7 B7 V. Description of the invention (139) Same as 64%. Example 18 Control of leaf damage caused by European corn perforator on corn plant by spray application of bronchial (W-14 strain) broth to reduce bacillus toxins printed by the Consumer Cooperative of Central Standard Bureau of the Ministry of Economic Affairs The ability of insect larvae to damage plants is demonstrated by measuring leaf damage caused by European corn perforators from parasitic to corn plants treated with bacilliform broth. Fermented broth from the strain of Corynebacterium W-14 was produced and concentrated to about 10 times by ultrafiltration (10,000 MW pore size) as described in Example 13. The resulting concentrated broth was subsequently filtered and sterilized using a 0.2 micron nitrocellulose membrane filter. A similar preparation of uninoculated 2% Proteoglycan No. 3 was used as a control. In a greenhouse (27GC during the day; 22GC at night, about 50% RH, 14 hours of day length, washing / fertilizing as needed), corn plants (a close-breeding line) grow from seed to 7th in a pot containing a soilless mixture Or 8 growing season. The test plants are in a randomized complete block design (randomized complete block design) in a greenhouse with about 22GC during the day, about 18GC at night, no artificial light, with partial shading, about 50% RH, and watering / fertilizing as needed. 3rep / treatment, 6 plants / treatment). Treatment (uninoculated medium and concentrated bacilliform broth broth) applied with a syringe sprayer, 2.0ml directly (about 6 inches) was applied to the whorl (whorl), and another 2.0ml in the round Approximately 1 foot above the body was applied in a circular motion. In addition, one group of plants recovered was untreated. After the treatments were dried (approximately 30 minutes), 12 newborn European corn perforator larvae (eggs were obtained from commercial sources and hatched indoors) were placed directly into the rotaform. After 1 week, these plants used the modified Guthrie Scale to -142- This paper size applies the Chinese National Standard (CNS) A4 specification (210 X 297 mm) 509722 A7 B7 V. Description of the invention (140 ) The damage of these blades is scored (Koziel, M.G., Beland, G.L., Bowman, C., Carozzi, NB, Crenshaw, R., Crossland, L., Dawson, J., Desai, N., Hill, M., Launis, L, Lewis, K., Maddox, D., McPherson, K., Meghji, Μ. Z., Merlin, E., Rhodes, R., Warren, GW, Wright, M. and Evola, SV1993 · Bio / Technology, 11, 194-195), these scores are compared statistically [T-test (LSD) p <0.05; Tukey's Studentized Range (HSD) test ρ < 0 · 1]. The results are shown in Table 29. For reference, 1 point means no damage, 2 points means slight “pane” damage but no perforation on uncurled leaves, and 5 points means more than 3 leaves have extended lesions and / or central vein spraying Leaf perforation (lesions less than 1 inch). These data show that when applied in a sprayable formula or when the gene or derivative encoding the protein or part thereof is spread through a gene transgenic plant or microorganism At that time, broth or other protein-containing isolated parts can provide protection against specific insect pests. Hole 29 bacillus flesh: prepared for the effect of buckle on printed leaves printed on corn by the Consumers Cooperative of the Central Standards Bureau of the European Jade Ministry of Economic Affairs The average Gorilla score of the bacilliform broth of the untreated and uninoculated medium was 5.02a 5. 15 a 2.24 b The average values with different letters were statistically different (ρ < 〇 · 〇5 or ρ < 0 · 1) 〇 Example 19-Extraction of the gene construction in E. coli f-143- This paper is suitable for financial standards and family standards iTcNS) A4 regulations i72H) x297: ^ Printed by the Consumers' Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs 509722 A7 B V. Description of the Invention (141) A series of plastids were constructed to express the icW gene of Bacillus w-14 in E. coli. A list of such plastids is shown in Table 30. A brief description of each plastid The summary with the obtained performance data of the E. coli is continued. Table 30 The pupid-gene vector / selected expression site PDAB2025 tcbA pBC / Chl PDAB2026 tcbA pAcGP67B / Amp baculovirus 'Secret PDAB2027 tcbA pET27b / Kan Perinuclear cytoplasm PDAB2028 tcbA pET15-icM Abbreviations in the cell: Kan is kanamycin, Chi is amycin, and Amp is ampicillin. The construction of PDAB2025 is described in Example 9 and Large & slave / fragment of TcbAu probe hybridization. This fragment was sub-selected into pBC (Stratagene, La Jolla, CA) to produce PDAB2025. Sequence analysis showed that the fragment was 8816 base pairs. This fragment encodes the gene, It has the starting ATg at position 571 and the terminating TAA at position 8086. Therefore, the fragment carries 570 base pairs of Corynebacterium DNA upstream of the ATG and 730 downstream of the TAA Base pair The gene of PDAB2026 was amplified from pDAB2025 plastid by PCR using the following primers: 5 'primer (SlAc51) 5' TTT AAA CCA TGG GAA ACT CAT TAT CAA GCA CTA TC 3 'and 3' primers (SlAc31) 5'TTT AAA GCG GCC GCT TAA CGG ATG GTA TAA CGA ATA TG 3 ,. The PCR system uses a standard from • 144- This paper is sized to the Chinese National Standard (CNS) A4 (21 OX297 mm) t # " Read the precautions on the back before filling this page) r 509722 A7 B7 V. Description of the invention ( 142)

The TaKaRa LA PCR kit of PanVera (Madison, WI) was performed in the following reactions: 57.5μ1 water, 10μ1 10X LA buffer, 16μ1 dNTPs (2.5mM for each concentrate), 20μ1 primers (each primer was lOpmoles / μΙ), 300ng of the plastid pDAB2025 and 1μ1 containing the W-14 icM gene & 1 > 1 ^ 1 ^ 1 ^^ & 9 polymerase. The cycling conditions were 98 () (: / 20 seconds, 68 () (: / 5 minutes, 72QC / 10 minutes for 30 cycles. One of the expected approximately 7526bp PCR products was in a TBE (100mM Tris, 90mM boric acid). , ImM EDTA) buffer was separated from a 0.8% agarose gel and purified using a Qiaex II kit from Qiagen (Chatsworth, CA). The purified gene was digested at σ / and / ί / , Ligated into the baculovirus delivery vector pAcGP67B and transformed into DH5a E. coli. The resulting recombinant was called PDAB2026. The 纟 cM gene was then excised from pDAB2026 and transferred to pET27b to generate pDAB2027 plastids A missense mutation in the icW gene is repaired in pDAB2027. The repaired plus gene contains two changes that are different from those shown in sequence identification number 11; A changes to G at 212 Change asparagine 71 to serine 71 and G to A at 229 change alanine 77 to threonine 77. These changes are all upstream of the putative TcbAHN-end. Employees of the Central Bureau of Standards, Ministry of Economic Affairs Printed by Consumer Cooperatives (Please read the notes on the back before filling out this page) PD The icM coding region of AB2028's hibiscus PDAB2027 was transferred to the pET15b vector. This was achieved using shotgun-type ligation, and the DNA was restricted by the enzyme σ / and / cut. The resulting recombinant was called pDAB2028. Performance of Escherichia coli internally derived from DDAB2028 plastid TcbA

The performance of TcbA in Escherichia coli is described previously. _ -145 -__ This paper size is applicable to the Chinese National Standard (CNS) A4 specification (210X297 mm). A7 B7 V. Description of Invention (⑷)

Studierei 3 (Studier, FW ·, Rosenberg, A ·, Dunn, J · and Dubendorff, J., (1990) Use of T7 RNA polymerase to direct expression of cloned genes · Methods Emzymol., 185: 60-89) Modifications of these methods were obtained. A competent E. coli cell BL21 (DE3) strain was transformed with pDAB2028 plastid and spread on LB lipids containing 100 pg / ml ampicillin and 40 mM glucose. The transformed cells are coated at a density of several hundred independent colonies per plate. After incubation at 37GC overnight, the cells were scraped from the plates and suspended in LB broth containing 100 pg / ml ampicillin. A typical culture volume is 200-500ml. Initially, the culture density (OD600) depends on the experiment from 0.005 to 0.15. The culture was shaken at one of 22QC, 30GC or 37GC until a density of 0.15-0.5 was obtained, at which time it was induced with ImM isopropylthio-B-galactoside (IPTG). The culture was incubated at the indicated temperature for 4-5 hours and then moved to 4GC until processing (12-72 hours). Purification of TcbA from DDAB2028 expressed in E. coli ^^ The E. coli culture conferring TcbA peptide was processed as follows. Cells were harvested by centrifugation at 17,000xg, and the medium was decanted and stored in other containers. The culture medium was concentrated about 8 times using the M12 (Amicon, Beverly, MA) filtration system and a 100 kDa molecular weight blocking filter. The concentrated culture medium was packed into an anion exchange column and the bound proteins were eluted with 1.0 M NaCl. The 1.0M NaCl elution spike was found to cause death of the southern corn rootworm (SCR) (Table 30). The 1 · 〇M NaCl separation part is -146- This paper size applies to China National Standard (CNS) A4 specification (21〇297mm) (Please read the precautions on the back before filling this page) • Packing. 4 509722 A7 ____ B7 _ _ 5. Description of the invention (144) 10 mM sodium linate buffer (pH 7.0) was dialyzed, concentrated, and subjected to gel filtration in Seperose CL-4B (Pharmacia, Piscataway, NJ). Areas corresponding to the calculated molecular weight (approximately 900 kDa) such as the CL-4B eluted image of the natural W-14 toxin complex were collected, concentrated, and subjected to biological analysis against larvae. The collected 900 kDa isolated part was found to have insecticidal activity (see Table 31 below), and its symptomology was similar to that caused by the natural W-14 toxin complex. This separated part was subjected to proteinase K and heat treatment, and the activity was removed or reduced in two examples, which provided evidence that the nature of the activity was proteinaceous. Furthermore, when tested with a single-antibody-TcbA single-source antibody, the test of the active fraction against the TcbA and TcbAUi peptides in immunological rupture analysis was immunologically positive (Table 31). 131 Immune results of Han Qing'ao SCR biodiversity (Please read the precautions on the back before filling this page)

、 1T Printed and separated part SC by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs] Natural active immune disruption Natural size% Mortality% Growth inhibition tested peptide [CL-4B estimated size] TcbA medium 1.0M Ion exchange ++ + +++ TcbA TcbA medium CL-4B +++ +++ TcbA, TcbA iii About 900kDa TcbA medium CL-4B + protease K ++ +++ NT TcbA medium CL-4B + heat treatment — one NT TcbA cell supernatant CL-4B +++ NT about 900 kDa PK = proteinase K treatment for 2 hours; heat treatment = 100 GC for 10 minutes; ND = J. Not detected; NT = Not measured. The scoring system for mortality and growth inhibition is compared with the control sample, 5-24% = ,, + ,,, 25-49% = ,, ++ ,,, 50-100% = ,, + ++ ,,. ___ -147-The paper size is 21 × 297mm)-A7 _______B7 V. Description of the invention (145) (Please read the precautions on the back before filling this page) The cell pellet was resuspended in 10mM phosphoric acid Sodium buffer (ρΗ7 · 〇) and lysed by passing through a Bio-NebTM cell sprayer (Glas-Co1 Inc., Terra Haute, IN). The pellets were treated with DNAse to remove DNA and centrifuged at 17,000 × g to separate the cell pellets from the cells. The supernatant fraction was decanted, filtered through a 0.2 micron filter to remove large particles, and subjected to anion exchange chromatography. The bound protein was eluted with 1.0 M NaCl, dialyzed, and 61 × 11 ^ 1 ^ (^ 1110〇 『60〇 ^, 56 (1 € 〇 ((11,11)) The concentrator was concentrated. The concentrated separated part was gel-filtered using Sepharose CL-4B bead matrix. The bioanalytical data of the substances prepared in this way are shown in Table 30 and shown in Table 30. Supernatant of TcbA cells, said. In another method of handling large amounts of substances printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs, these cell pellets were resuspended in 10 mM sodium acid buffer (ρ 纳 7 · 0). And homogenized thoroughly using a 40ml tissue mill from Kontes Glass (Vineland, NJ). The cell debris was pelleted by 25,000xg centrifugation, the cell supernatant was decanted, passed through a 0.2 micron filter and Anion exchange chromatography was performed on a Pharmacia 10/10 column packed with Poros HQ 50 beads. The bound protein was eluted by performing a NaCl gradient of 0.0 to 1.0M. The TcbA protein fraction was mixed, Concentrate it with a 50kDa concentrator and use Pharm The acia CL-4B bead matrix was subjected to gel filtration chromatography. The separated fractions containing TcbA oligomers (molecular weight of approximately 900 kDa) were collected and used in Pharmacia Mono Q 10 equilibrated with 20 mM Tris buffer (ρΗ7.3). Anion exchange chromatography was performed on a 10-column column. A NaCl gradient of 0.0 to 1.0 μM was used to elute the recombinant TcbA protein. The recombinant TcbA was from this tube -148-This paper is in accordance with Chinese National Standard (CNS) A4 Specifications (210X297 mm) Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 509722 A7 __B7 V. Description of the Invention (146) The column was eluted at a salt concentration of about 0.3-0.4MNaCl, which is a natural oligomer with natural TcbA oligomers. The Mono Q 10/10 column was eluted with the same number of moles. The recombinant TcbA fraction was found to cause SCR death in a bioanalysis experiment similar to Table 31. Group 2 performance constructs were prepared and tested. The expression of tcbA protein toxin. The construction of PDAB2030: one of the coding regions expresses the PDAB2028 plastid (see this article) in the commercial pET15 vector (Novagen, Madison, MI; it encodes an ampicillin selection marker) Coding region. The pDAB2030 plastid was created to express the plastid plus coding region by encoding one of the kanamycin selection markers. This cuts a kanamycin selection plastid pET27 (Novagen, Madison, MI) and PDAB2028 with / add / and / Λσ /. This releases the complete multiple colony site, which includes the icW coding region from PDAB2028. The cut two kinds of plastids are mixed and connected. Recombinant plastids were selected with kanamycin, and those who contained the PDAB2028 fragment were identified by restriction enzyme analysis. This new recombinant plastid is called PDAB2030. Construction of PMB2031 plastid: Chromium mutation in the pupae, as described in Example 19, the two mutations in the N-terminus of the coding region (sequence identification number 11; A changed to G at 212 to transform Asparagus Amine 71 was changed to serine 71; G to A at 229 changed alanine 77 to threonine 77) was modified as follows: a PCR product using the TH50 primer (5, ACC GTC TTC TTT ACG ATC AGT A 3 G ,) And SlAc51 primers (5, TTT AAA CCA TGG GAA ACT CAT TAT CAA GCA CTA TC 3,) and pDAB2025 -149- This paper size applies the Chinese National Standard (CNS) A4 specification (210X297 mm) (Please read first Note on the back, please fill in this page again.) I. Binding and printing. Printed by the Consumers' Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs. 509722 Α7 Β7. 5. Description of the Invention (I47) is manufactured as a template to produce a 1778bp product. This PCR product was cloned into pCR2.1 plastids (Invitrogen, San Diego, CA), and one strain was isolated and sequenced. The strain was digested with σ / and ZV / 7〆 /, and a 1670bp fragment was purified from a 1% agarose gel. A plastid (PDAB2030) containing the icW coding region of the mutation was digested at σ / and ί / and purified by Qiaex II (Qiagen, Chatsworth, CA) of the 1670bp fragment in 0.8% leptose come out. The modified price σ /// V ;? segment is then connected into PDAB2030. The ligated DNA is transformed into DH5a E. coli. One strain was isolated and sequenced and found to be modified. This plastid containing the modified ic plus coding region is called pDAB2031. Construction of PDAB2033 and PDAB2034: Performance plastids The PDAB2025 and pDAB2027-2031 performance plastids all depend on the T7 phage expression system. Another vector system was used for bacterial expression of the ic plus gene and its derivatives. The Trc99a expression vector (Pharmacia Biotech, Piscataway, NJ) contains a strong trc initiator upstream of a multiple breeding site containing a 5 'claw / site, and the 5' ^ σ / site is identical to that from pDAB2030 and 2031. Coding regions are compatible. However, the plastid does not have a compatible 3 'site. Therefore, the Trc99a and / Λί / / 77 sites were excised and blunt-ended by T4 DNA polymerase (Boehringer Mannheim, Indianapolis, IN). The vector plastid was then cleaved by # co / followed by treatment with alkaline phosphatase. The PDAB2030 and 2031 plastids were cut with //? // respectively (cut at the 3 'end of the icW coding region), and then treated with alkaline phosphatase to dull the ends. The plastids were subsequently cut at a valence σ /. The DNA was extracted with phenol, precipitated with alcohol and resuspended in buffer. -150- This paper size applies to Chinese National Standard (CNS) Α4 size (210 × 297 mm) '' (Please read the precautions on the back before filling this page)

509722 A7 B7 V. Description of the invention (148) The Trc99a and pDAB2030 and 2031 plastids were mixed, connected, and sent to DH5ot cells for transformation and coating on LB medium containing ampicillin and 50 mM glucose. Recombinant plastids were confirmed by restriction enzyme digestion. The new plastids are called pDAB2033 (containing the coding region with 2 mutations included) and pDAB2034 (containing the modified version from pDAB2031). A hibiscus signature for PDAB2032: one that expresses the T (^ ΑΗΑΗ3Ρ) plastid that encodes TcbA in the same way as one of PDAB2031. A PCR product uses TH42 (5, TAG GTC TCC ATG GCT ΤΤΤ ΑΤΑ CAA GGT TAT AGT GAT CTG 3,) and TH50 (5, ACC GTC TTC TTT ACG ATC AGT G 3,) primers and printed with PDAB2025 Employee Consumer Cooperative of the Central Standards Bureau of the Ministry of Physics and Economics (please read the precautions on the back before filling this page) ) As a template. This produces a 1521 bp product with a start codon at the beginning of the coding sequence of TcbAii. The PCR product is isolated and purified in a 1% agarose gel. The purification The product was cloned into pCR2.1 as above, and a correct strain was identified by DNA sequence analysis. This strain was digested with / and / V / 7 d /, and a 1414bp fragment was isolated in a 1% agarose gel. The lei? / And / Vi? # Address' connected to the PDAB2030 plastid was sent to DH5a E. coli for transformation. This new plastid designed to express TcbAuAiu in E. coli was called PDAB2032. Derived from dDAR training, dDAB2031 and PDAB2032 plastids The performance of Jia and Tu ·, ·, · • --- M, originated from the addition of PDAB2030, PDAB2031, and pDAB2032 plastids, and their performance in E. coli, as revealed and described here, except “You” Expressed in Escherichia coli HMS174 (DE3) strain (Novagen, • 151- This paper size is applicable to Chinese National Standard (CNS) A4 specifications (210X 297 mm)) Printed by the Ministry of Standards Bureau's Consumer Cooperatives 509722 5. Description of the invention (⑷ )

Madison, WI). Observation from pDAB2033 plastids This pDAB2033 plastids were delivered into blu cells (Novagen, Madison, wi) to be transformed and coated with iO (^ g / ml ampicillin and 50mM glucose) LB. After overnight incubation at 300c or 37oC, the plates were coated so that hundreds of well-isolated colonies appeared on each plate. The strains were scraped from the plates and Suspension in LB containing 10 μg / ml ampicillin but no 50 mM glucose. A typical culture volume in a single il throttling flask is 250 ml. When the culture reaches a density of one 〇66, the cultures are Induction. Usually this density is reached quickly after suspension of the cells from the trays without the need for a period of growth in the liquid culture medium. 2 induction methods are used. Method 1 · The cells are at lmM at 37 ° C IpTG induction. The cultures were shaken at 200 rpm for 5 hours on a platform shaker and then collected. Method 2: The cultures were induced at 30 μC with 25 μM IpTG and at 20 rpm or 200 rpm at 200 rpm. Shake for 15 hours. The cultures are stored under 4GC until used for purification. .

TcbA from the silk of E. coli

The purification, biological analysis, and immune conversion of TcbA and T (^ Α 免疫 ΑΗί are not explained here. The results of several representative E. coli performance experiments are not shown in Table 32. All the materials shown in Table 32 are purified A portion was separated from the cultured medium. The predicted natural molecular weight is approximately 900 kDa as disclosed herein. The purity of the samples and the amount of TcbA relative to the promiscuous protein are different for each preparation. ~ Intestine TcbA Yuyan 152 purified from this culture base is invited to be used in the stalk fungus. This paper size is suitable for financial specifications (21〇 > < 297) | (Please read the precautions on the back before filling this page) Order 509722 Α7 ______ Β7_ V. Description of the invention (150) Biological bioanalytical activity of organisms and pain-free spines to separate the plastid coding region Escherichia coli Southern corn rootworm bioanalytical activity The protein in the feed με% growth inhibition by immunopeptide determination % Mortality PDAB2030 tcbA BL2KDE3) a +++ TcbA + TcbAin 1-8 PDAB2031 tcbA BL21 (DE3) pain +++ TcbA + TcbAiii 1-10 PDAB2033 tcbA BL21-+++ TcbA + TcbAiii 1- 2 PDAB2032 tcbAiiAm H 74 (DE3) +++ + TcbAi i Ai i i + TcbAj ii 12-27 The scoring system for the mortality and growth inhibition of southern corn rootworms is compared with the control sample, 5-24% = ”+ ”， 25-49% =” ++ ”， 50-100% = ，， +++ ，，. Example 20 Substrate-assisted laser desorption and hand-assisted mass spectrometry Λ 丨 / 1 ---- --- •• I-- (Please read the notes on the back before filling out this page) The toxin peptide characteristic printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs. The toxin isolated from W-14 broth is an example. It was purified as described in 15. In some examples, the TcaB protein toxin was pretreated with a protease (Example 16) that has been isolated from W-14 broth as previously described (Example 15). The molecular weight of the protein is used in Matrix-assisted laser desorption ionization time-of-flight mass spectrometer (hereinafter, MALDI-TOF) on a VOYAGER BIOSPECTROSCOPY workstation, one of the DELAYED EXTRACTION technologies (PerSeptive Bioysytem, Framingham, MA). Typically, the protein of interest (100-500 pmoles in 5 μl) mixed with 1 μΐ acetonitrile and a Mill with a pore size of 0.025 μM ipore VS fermenter (Mil 1 ipore Corp. Bedford, MA). The dialysis was carried out as follows: the filter was floated on the water (smooth side up), and then the protein-acetonitrile mixture was added dropwise to the surface of the filter. After dialysis, the dialyzed protein was sucked up with a burette, and then it was ordered with 509722 A7 B7 according to the manufacturer's -153- this paper size applies Chinese National Standard (CNS) M specifications (210X297 mm) V. Description of the invention (151) 'Indicate that the matrix composed of erucic acid and triacetic acid is mixed. The protein and matrix were allowed to co-crystallize on a gold plate (PerSeptive Corp.) of approximately 3 cm2. The mass analysis of the activation and continuity of these crystals was performed using the following conditions: laser setting of 3050, pressure of 4.55e-07, low mass grid of 1500.0, no ion removal, acceleration voltage of 25,000, 90.0% Gate voltage, 0.010% wire voltage, linear mode, and 350 ns ripple delay time. The protein quality analysis data is shown in Table 33. The data obtained from MALDI-TOF was compared with those hypothesized from genetic sequence data and previously determined by SDS-PAGE. Table 33 Peptide breakup by MALDI-TOF, SDS-PAGE, and gene sequence-based prediction

Peptide Prediction SDS-PAGE MADLI -T0F (Please read the precautions on the back before filling this page)

A A A A b b b b c c c c T T T T 280, 634 Da 217,710 Da 207,698 Da 62,943 Da 240.000 Da Not privately analyzed 201.000 Da 58,000 Da 281,040 Da 216,812 Da 206,473 Da 63,520 Da

TcdA TcdA

TcbA 209, 218 Da 63,520 Da 188.000 Da 56.000 Da 208,186 Da 63,544 Da Protease producer 201,000 Da Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs

TcbAm protease producer 56,000 Da 216,614 Da ^ 215,123 Da, 208,680 Da '64,111 Da ^^ Data standardized for TcbA, multiple fragments observed in TcbAi / H. Example 21 Production of peptide-specific winter tear antibodies The nine winning components of the W-14 toxin complex, namely TcaA, TcaAiii, TcaBi, TcaBH, TcaC, TcbAH, TcbAHi, were selected as the generated antibody against Goal. A large amount of DNA of these peptides and -154- This paper size applies Chinese National Standard (CNS) A4 specification (210X297 mm) 509722 A7 B7 V. Description of the invention (l52) The amino acid sequence data deduced shows that some This sequence homogeneity between these peptides is true. If a complete peptide is used as an immunogen to induce antibody production, the resulting antibody may bind to multiple peptides in the toxin preparation. To avoid this problem, antibodies should be generated to bind to a unique region of each peptide of interest. The unique region (sub-peptide) of each target peptide is selected based on the aforementioned analysis. Printed by the Consumer Cooperatives of the Central Bureau of Standards of the Ministry of Economic Affairs (please read the notes on the back before filling this page). Each complete peptide sequence uses the MacVectorTM protein analysis tool (IBI sequence analysis software, International Biotechnologies, Inc., P · 0 · Box 9558, New Haven, CT06535) analysis to determine its antigenicity index. This program is designed to locate amino acid sequences that may be located on the outside, that is, regions that may be antigenic locations. This method combines data from hydrophobicity, surface probabilities, and backbone elasticity predictions along with secondary structure predictions to generate a compositional prediction of the surface profile of a protein. The scores for each of these analyses were normalized to values between -1.0 and +1.0 (MacVectorTM Handbook). The antigenicity index value is obtained from the complete sequence of the target peptide. A region from each peptide covering 19 or more amino acids (which shows a high antigenicity index from the original sequence) was reanalyzed to determine the absence of flanking residues. The antigenicity index of this win. Since the immunogenicity index of Ichase is affected by the amino acid residues on both sides, this reanalysis is necessary. If the isolated peptide sequence fails to maintain a high antigenicity index, a new region is selected and the analysis is repeated. Each selected secondary peptide sequence is aligned and compared using the 仏 3 (^ 6 (: 1; 01 ^ permutation program) to compare all 7 target peptide sequences. If a selected secondary peptide sequence shows a relationship with • 155- This paper size applies to Chinese National Standard (CNS) A4 (210 X 297 mm) 509722 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs A7 B7__ 5. Description of the invention (153) The identity of other target peptides (greater than 20%) ), A new 19 or more amino acid region was isolated and reanalyzed. The unique subpeptide sequence covering 19 or more amino acids showed a high antigenicity index selected from all target peptides The sequence of the 7 sub-peptides was sent to Genemed Biotechnology Inc .. The last amino acid residue on each sub-peptide was deleted because it had no significant effect on the antigenicity index. Half of the deamino acid residues The base is added to the N-terminus of each peptide sequence, except for TcaBi-syn, which contains a cysteine residue. The presence of a cysteine residue promotes the binding of a carrier protein (KLH). The final peptide product and sequence corresponding to the appropriate toxin peptide The identification numbers are shown in Table 34. Table 34 Synthetic peptide amino acid sequence sequence identification number peptide amino acid sequence 63 TcaAn-syn ~ NH2-(C) LRGNSPTNPDKDGIF AQ VA ~ 64 TcaAiH-syn NH2- (C) YTPDQTPSFYETAFRSADG 65 TcaBrsyn NH2-HGQSYNDNNYCNFTLSINT 66 TcaBiirsyn NH2- (C) VDPKTLQRQQAGGDGTGSS 67 TcaC-syn NH2- (C) YKAPQRQEDGDSNAVTYDK 68 TcbAH-SYYYYCNYYCNY-CNY2 (C) YNENPSSEDKKWYFSSKYNACDANH2-C TcdAiirsyn NH2- (C) VSQGSGSAGSGNNNLAFGAG Each bound synthetic peptide was injected into 2 rabbits according to the Genemed Express Plan. The pre- and post-immunization sera were available for testing after 1 month. Taken from each rabbit This preparatory test with post-immunization sera was performed by Genemed Biotechnologies Inc. Genemed published its use of ELISA and Western deflation technology to detect the response of immunized sera to their respective synthetic peptides. Then, these sera were analyzed by Western deflation analysis and Complete -156 · (Please read the precautions on the back before filling this page)

, ^ T __. This paper size is applicable to the Chinese National Standard (CNS) A4 specification (210X 297 mm) 509722 A7 ____B7___ 5. Description of the invention (l54) The target peptide is tested. Two batches of partially purified Corynebacterium w-14 strain toxin complexes were used as antigens. The two samples have shown activity against the southern corn rootworm. The peptide pattern on a SDS-PAGE gel is slightly different. Printed by the Consumer Cooperatives of the Central Bureau of Standards of the Ministry of Economic Affairs (please read the precautions on the back before filling this page). SDS-Polypropylene amine gel (Integrated Separation Systems, natick, MA01760) with a 4-20% gradient is use. 1 to 8 pg of protein was added to each gel well. Electrophoresis was performed and the protein was electroporated onto a Hybond-ECLTM nitrocellulose membrane (Amersham International). The membrane was blocked with 10% milk in TBST (25 mM Tris-HC1, pH 7.4, 136 mM NaCl, 2.7 mM KC1, 0.1% Tween 20) for 1 hour at room temperature. Each rabbit serum was diluted to 1: 500 with 10% milk / TBST. Other dilutions between 1:50 and 1: 1000 are also used. The serum was added to the membrane and placed on a platform shaker for at least 1 hour. The membrane was washed thoroughly with blocking solution or TBST. A 1: 2000 diluted secondary antibody (goat anti-mouse IgG bound to peroxidase) dissolved in 10% milk / TBST was added to the membrane on a platform shaker for 1 hour. . The film was then washed with a large amount of TBST. The detection of the protein was performed by using an ECL (Promoting Chemiluminescence) Detection Kit (Amersham International). Western rollover analysis was performed to confirm the binding specificity of each anti-synthetic antibody. All synthetic multi-source antibodies showed specificity to treated and untreated (when applicable) target peptides (the target peptides were derived from the protein-isolating portion of the bacillus culture broth). Different antibodies show that it can recognize and derive from cells such as bacteria or insect cells (Lee-157- This paper size applies Chinese National Standard (CNS) A4 specifications (210X29 * 7) & 509722 A7 B7 V. Description of the invention (155) (Viral constructs) Heterologous expression system of treated and untreated recombinant proteins. In one example, the anti-TcbAHi-syn antibody showed some cross-reactivity with the anti-TcdAiU-syn peptide. In Section 2 In one example, the anti-TcaC-syn antibody recognized a 190 kDa peptide that was not recognized in the isolated part of the W-14 toxin complex. ^ J22 The characteristics of the strain of Pseudomonas aeruginosa were printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs. (Please read the precautions on the back before filling out this page) In order to prove that the collection disclosed here contains the strain of Bacillus bacillus, these strains in this article are based on the identified microbiological traits (which is the bacterium Bacillus genus Characteristics and can be distinguished from other enterobacteriaceae and heterobacteria) (Farmer, JJ 1984. Bergey's Manual of Systemic Bacteriology, Vol 1. pp. 510-511. (Ed. Kreig, NR and Holt, JG). Williams & Wilkins, Baltimore; Akhurst and Boemare, 1988, J. Gen. Microbiol. 134, 1835-1845; Forst and Nealson, 1996. Microbiol. Rev. 60, 21-43). This The unique traits are as follows: Gram-staining negative bacilli, biological dimensions of 0 · 3-2μm in width and 2-10μm in length [sometimes filamentous (15-50μπο and spheroplasts)], in nutrient agar The yellow to orange / red colony pigments are produced, crystal inclusions are present, catalytic enzymes are present, no nitrate reducing ability is present, bioluminescence is present, the ability to take up dyes from the growth medium, protease formation is positive, and the growth temperature is below 37 ° C, can survive under anaerobic conditions and laminar mobility (Table 20) 0 The test method uses references of Escherichia coli, heterobacteria and bacillus strains. The overall results are related to Enterobacteriaceae and bacillus All strains of the genus Mycobacterium are the same. DEP1, DEP2, and DEP3 are obtained from the American Stereotype Culture Collection -158-This paper is in accordance with the Chinese National Standard (CNS) A4 (210X297 mm) 509722 A7 ____B7 Description (say) (12301 Parklawn Drive, Rockville, MD 20852 USA) of Photorhabdus strain (# 29304, respectively, # 29999 and # 51583). A photometer was used to demonstrate the bioluminescence of this Corynebacterium strain. In order to measure the presence or absence of relative luminescence units, these broths from each strain (cells and media) were measured at 3 time intervals (24, 48, and 72 hours) after inoculation in liquid culture and compared with background luminosity (Uninoculated medium). Multiple strains of Corynebacterium were used as negative control groups to test for luminescence. Before measuring the radiation of light from different broths, the cell density was confirmed by measuring the absorbance (560nm) in a Gil ford Systems (Oberl in, OH) spectrometer using a siphon cell. The resulting luminescent unit is then normalized to the density of the cells. Portions of these broths were placed in 96-well microtiter plates (100 μ1 each) and interpreted in a Packard LumincountTM photometer (Packard Instrument Co., Meriden, CT). Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (please read the precautions on the back before filling this page) The measurement period for each sample is 0.1 to 1.0 seconds. The samples were stirred in the photometer for 10 seconds before being read. A positive test was determined to be about 5 times the background luminosity (about 1-15 relative luminescence units). In addition, the degree of colony luminosity was confirmed by covering with photographic film and reviewing the film in a dark room. These Gram staining characteristics of each strain were confirmed by the combined use of a commercial Gram staining kit (BB1, Cockeysville, MD) and a Gram stain control slide (Fisher Scientific, Pittsburgh, PA). . Microscopic examination was then performed using a Zeiss microscope (Carl Zeiss, Germany) 100X oil lens (magnified with 10X eyepiece and 2X lens body). The microscopic examination of the biological size, cell characteristics and inclusion bodies of the individual strains (the latter two were observed after logarithmic growth) uses a wet wipe (10X eyepiece, 2X lens body-159-). This paper standard applies to Chinese National Standard (CNS) Α4 Specifications (210X 297mm) 509722 Employees' cooperative of the Central Bureau of Standards of the Ministry of Economy printed A7 B7 V. Description of the invention (l57) Magnification and 40X objective lens) and phase difference microscope inspection with micrometer (Akhurst, RJ and Boemare, Ν · Ε · 1990 · Entomopathogenic Nematodes in Biological Control (ed. Gaugler, R · and Kaya, Η ·) · pp · 75-90 · CRC Press, Boca Raton, USA .; Baghdiguian S., Boyer-Giglio, H., Thaler, J. 0., Bonnot, G., Boemare, N. 1993. Biol. Cell 79, 177-185). Colony pigmentation was observed after inoculation on Bacto nutrient agar (Difco Laboratories, Detroit, MI; prepared as indicated by the label). Inoculation was performed at 28GC, and these characteristics were generated after 5-7 days. To test the presence of the enzyme-catalyzed enzyme, a colony of the test organism was prepared from a nutrient agar plate and placed on a small plug, and placed on the bottom of a glass test tube. Add 1 ml of household hydrogen peroxide solution gently along the side of the tube. A positive reaction was recorded when bubbles (assuming oxygen) appeared immediately or within 5 seconds. A control group of uninoculated nutrient agar and tritium peroxide solution was also tested. To test for nitrate reduction, each culture was inoculated into 10 ml of Bacto nitrate broth (Difco Laboratories, Detroit, MI). After incubation at 28 ° C with gentle agitation for 24 hours, the formation of nitrite was achieved by adding 2 drops of sulfanilic acid reagent and 2 drops of α-2-naphthylamine reagent (see Difco Manual, 10th edition, 'Difco Laboratories, Detroit, MI , 1984). The production of a unique pink or red color indicates the formation of nitrite from nitrate, while the formation of a color indicates that the strain is negative for nitrate reduction. In the latter example, finely divided zinc was added to further confirm the presence of unreduced nitrite, which was evidenced by the formation of nitrite and the resulting red color. The ability of each strain to take up dye from the growth medium _____ -160 _ This paper size is applicable to the Chinese National Standard (CNS) A4 specification (210X297). First (please read the precautions on the back before filling this page). 、 11 d-/ 1 509722 Printed by A7 B7_ of the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 5. Description of the Invention (l58) Bacto MacConkey soil grease containing neutral red dye, Bacto Tergi tol containing mothymol blue dye -7 agar and eosin-Y dye-containing Bacto EMB soil fat (prescription agar from Difco Laboratories, Detroit, MI, all prepared according to label instructions) were tested. After inoculation on these media, dye uptake was recorded after 5 days of incubation at 28GC. Growth on these latter media is characteristic of members of the Enterobacteriaceae. The motility of each strain was tested using a Bacto Motility Test Medium (Difco Laboratories, Detroit, MI) solution prepared as described in the standard instructions. Each strain was subjected to a bottom puncture inoculation, and motility was judged microscopically by spreading a diffused growth area from the inoculation line. The protease was produced by using a Bacto gelatin plate (Difco Laboratories, Detroit, MI) made as described in the label to observe the hydrolysis of gelatin. Cultures were inoculated and the tubes or plates were incubated at 28GC for 5 days. Gelatin hydrolysis was then checked at room temperature (i.e. below 22 QC). To evaluate growth at different temperatures, the agar plate [2% No. 3 protein glycoprotein gland and 2% Bacto-Agar (Difco Laboratories, Detroit, MI)] in deionized water was streaked with a common source of inoculum . The plates were incubated at 20, 28, and 37 ° C for up to 3 weeks. The incubator temperature level is checked with an electric thermocouple and a thermometer to ensure a valid temperature setting. The oxygen demand of the Corynebacterium strain was tested as follows. The bottom puncture into the fluid sulfurized glycolic acid broth (Difco, Detroit, MI) was performed. The tubes were incubated at room temperature for 1 week, and the culture was then examined for patterns and extent of growth. The resazurin indicator shows the presence of oxidized media or the aerobic living area (Difco Handbook, 10th Edition, Difco Laboratories, Detroit, MI). From the measured light ____ -161 · This paper size is applicable to the Chinese National Standard (CNS) A4 specification (210X297 mm) (Please read the precautions on the back before filling this page); Binding and ordering ^ 09722 A7 _B7 V. Description of the invention (i) The growth zone experimental results obtained by the bacillus strain are consistent with those of a facultative anaerobic microorganism. In the case of unclear results, the final soil month concentration of the fluid sulfurized glycolic acid farm medium was raised to 0.75%, and the growth characteristics were re-examined. Table 35 Taxonomic makeup of Bacillus bacteria nM A * BCDEFG Η I jJ KLMN 0 p Q P. zealandica —T + + rd S + + + + + P0 + + + + + + P. hepialus one + + rd S + — + + + Y + + + + + + HB-Arg one + + rd S + one + + + + W + + + + + + + HB Oswego one + + rd S + one + ten + ff + + + + + + HB Lewiston — + + rd S + one + + + τ + + + + + + + K-122 one + + rd S + one + + + Υ + + + + + + + — HMGD one + + rd S + One + + + RD + + + + + + + Indicus One + + rd S + One + + + W + + + + + + + GD One + + rd S + — + + + YT + + + + + + PWH-5 One 4 * + rd S + one + + + Y + + + + + + + Megidis one + + rd S + one + + + R + + + + + + HF-85 one + + rd S + one + + + R + + + + + + A. Cows a + + rd S + — + + + PR + + + + + + + MP1 a + + rd S + — + + + T + + + + + + MP2 a + + rd S + One + + + T + + + + + + MP3 ** + + rd S + + + + R + + + + + + + MP4 + + rd S + one + + + Y + + + + + + MP 5 + + rd S + one + + + + PR + + + + + + GL98 + + rd S + one + + + W + + + + + + GL101 + + rd S + one + + + + W + + + + + + GL138 — + + rd S + one + + + + ff + + + + + + GL155 + + rd S + one + + + W + + + + + + GL217 — + + rd S + one + + + Y + + + + + + GL257 + + rd S + one + + + 0 + + + + + + DEP1 — + + rd S + one + + ff + + + + + + DEP2 + + rd S + — + + + PR + + + + + DEP3 + + rd S + + + + CR + + + + + + one * A = Grans stain, B = crystal contains Body, 0 bioluminescence, j) = cell shape, E = motility, F = nitrate reduction, G = presence of catalytic enzymes, H = -162- This paper size applies Chinese National Standard (CNS) A4 specifications ( 210X297 mm) ^ ----- (Please read the notes on the back before filling out this page) Order __ Printed by the Employees' Cooperatives of the Central Government Bureau of the Ministry of Economic Affairs 509722 Α7 Β7 V. Description of the invention (160) Gelatin hydrolysis, 1 = Dye uptake, J = Pigmentation on nutrient agar (some colors will change after the 5th day) 'K = Growth on EMB soil fat, L = Growth on MacConkey agar, M = Teritol- Growth on 7 soil lipids, N = facultative anaerobic, 0 = growth on 20GC, P = The growth 28QC, Q = 37QC the growth, + + / - = trait of positive or negative, RD = tuberculosis, S = sized within the range of GENUS. $ W = white, CR = cream, Y = yellow, YT = yellow brown, T = brown, P0 = light orange, 0 = dial, PR = light red, R = red. Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (please read the notes on the back before filling out this page) d The evolutionary ambiguity of these Bacillus strains in our collection uses genomic DNA from each strain, Measured by analysis of the fingerprinting of the genome of a PCR (polymerase chain reaction) vector. This technique is based on the fact that pedigrees of repeated DNA sequences are ubiquitous in the genes of heterogeneous bacteria (by Versalovic, J., Schneider, M., DE Bruijn, FJ and Lupski, JR 1994. Methods Mol. Cell · Biol., 5, 25-40. (Re-review). Three of these, the repeated extragenic palindromic sequence (REP), the intestinal bacteria repeat intergenic consensus sequence (ERIC), and the BOX factor are considered to play an important role in the composition of the bacterial genome. The composition of the genome is believed to be shaped by selecting the different distributions of these factors in the genes of closely related strains, which can be used to distinguish these strains (such as Louws, F. J., Fulbright, D.W., Stephens, CT and DE Bruijn, FJ 1994. Appl. Environ. Micro 60, 2286-2295). Rep-PCR uses complementary nucleotides to complement these repeats to amplify DNA fragments of different sizes in between. The obtained products were separated by electrophoresis to establish the DNA of each strain "means _ -163 · This paper size is applicable to the Chinese country; ^ (CNS) M specifications (21 × 297 mm) 509722 A7 B7 V. Description of the invention ( ⑹) To isolate genomic DNA from our strains, the cell pellet was resuspended in TE buffer (10mM Tris-HCl, ImM EDTA, ρΗ8.0 ·) to a final volume of 10 ml, and then added 12ml 5M NaCl. This mixture was centrifuged at 15,000 × g for 20 minutes. The resulting precipitate was resuspended in 5.7ml TE and printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (please read the precautions on the back before (Fill in this page) Add 300μ1 10% SDS and 60μ1 20mg / ml proteinase K (Gibco BRL Products, Grand Island, NY). This mixture was incubated at 37 ° C for 1 hour, and then 10mg lysozyme was added and incubated for 45 hours. Then, 1ml 5M NaCl and 800μ1 CTAB / NaCl solution (10% w / v CTAB, 0.7M NaCl) were added, and the mixture was incubated at 65GC for 1 hour, gently stirred, and then incubated and stirred for 20 minutes to promote the cellularity. Material clarification. An equal volume of aerosol / isoamyl alcohol solution (24: 1, v / v) was added and mixed gently and then centrifuged. Subsequently, an equal volume of phenol / aerosol / isoamyl alcohol solution (50: 49: 1) was used for 2 extractions Genomic DNA was precipitated with 0.6-fold volume of isoamyl alcohol. The precipitated DNA was removed with a glass rod, washed twice with 70% ethanol, dried and dissolved in 2 ml STE (10 mM Tris-HC1, pH 8.0; 10 mM NaCl, 1 mM EDTA). The DNA was then quantified by an optical density of 260 nm. The following primers were used for rep-PCR analysis of the DNA of the bacillus genome: REP1R-1; 5, -111ICGICGICATCIGGC-3 'and REP2-1: 5,-ICGICTTATCIGGCCT-AC-3 '. PCR was performed using the following 25μ1 reaction: 7.75μ1 Η20, 2.5μ1 10 × LA buffer (PanVera Corp ·, Madison, WI), 16μ1 dNTP mixture (2.5mM each) , Each primer 1μΐ (concentration 50ρM / μ1), 1μΐ DMSO, 1.5μ1 genomic DNA (concentration range 0.075-0.48 (^ g / pl) and 0 · 25μ1 TaKaRa EX Taq (PanVera -164- This paper size is applicable to China) Standard (CNS) A4 specification (210X 297 mm) 509722 A7 B7 V. Description of the invention (I62)

Corp., Madison, WI). The PCR amplification was performed in a Perkin Elmer DNA Thermal Cycler (Norwalk, CT) using the following conditions: 950C / 7 minutes, followed by 35 cycles of 94GC / 1 minute, 44GC / 1 minute, 65GC / 8 minute, and then at 65QC lasted 15 minutes. After cycling, the 25 μl reaction was added to 5 μl 6 × gel loading buffer (0.25% bromophenol blue in Η20, 40% w / v sucrose). A 15 X 20 cm 1% agarose gel was then taken and 8 μl of each reaction was run in TBE buffer (0.09M Tris-borate, 0.002M EDTA). The gel was run at 45v for about 16 hours. The gels were then stained in 2 μg / ml ethidium bromide for 1 hour and faded in TBE buffer for about 3 hours. Then Polaroid® photographs of these gels were taken under UV lighting. Central Bureau of Standards, Ministry of Economic Affairs Printed by the Employee Consumer Cooperative (please read the notes on the back before filling this page) The presence or absence of each strain in a specific size band is evaluated from these photos and included in a numerical taxonomy software program (NTSYS-pc; Exeter Software, Setauket, NY) was entered with a similarity matrix. A control group of E. coli HB101 strain and Xanthomonas ClantJiomonas oryzae pv. Or / zae) were analyzed at the same time to generate a PCR consistent with the published report. " Fingerprints "(Versalovic, J., Koeuth, T. and Lupski, JR 1991. Nucleic Acids Res. 19, 6823-6831; VeraCruz, CM ·, Halda-Alija, L ·, Louws, F., Skinner, DZ, George , ML, Nelson, RJ, DE Bruijn, FJ, Rice, C. and Leach, JE 1995. Int. Rice Res. Notes, 20, 23-24; VeraCruz, CM, Ardales, EY, Skinner, DZ, Talag, J . Nelson, RJ, Louws, FJ, Leung, H., Mew, TW and Lea ch, J.E. 1996.

Phytopathology). The data from Bacillus bacillus strains were subsequently analyzed at -165- This paper size applies the Chinese National Standard (CNS) A4 specification (210X: W mm) 509722 A7 B7 V. Description of the invention (163) A series of programs in NTSYS-pc (As follows): SIMQUAL (similarity of qualitative data) to generate a matrix of similarity coefficients (using Jaccard coefficients); SAHM clustering (Sequential, Agglomerative, heirarchical and Nested clustering) [using UPGMA (Unweighted Pair-Group Method with Arithmetic Averages) method can classify related strains and can be represented by an evolutionary tree (Figure 5). The C0PH (co-phenotypic value) and MXC0MP (matrix comparison) programs are used to generate a matrix of co-phenotypic values and compare the correlation between this value and the original matrix that is the basis of the cluster. As a result, a standardized Mant statistic (r) is generated, which is a measure of good fit for a cluster analysis (Γ = 0 8_ 0.9 represents an excellent fit). In our example, r = 0.924. Therefore, our collection contains representative strains that are divergent and easily distinguishable from this group of Bacillus species. Example 13 The original "strain" culture of different toxins produced by different toadstrains of Toadstool strains was based on ―first generation changes (please read the precautions on the back before filling out this page) ^ Install and set the central standard of the Ministry of Economic Affairs Heterogeneous clones printed by the Bureau ’s Consumer Cooperative were inoculated to 175ml of 2% No. 3 protein glycoprotein ^ (ρρ3. Difco Laboratories, Detroit, MI) liquid medium, which was sealed with Kaput and 500ml with one of Dilong's neck Three throttling bottles. After inoculation, these bottles were incubated on a convoluted stirrer at 150 rpm at 28 GC for 24 ~ ^ j until reaching the stationary phase. The culture was filled into a sterile bottle containing a sterile magnetic stir bar, and the culture was covered with a mineral oil to limit exposure to air. The stored culture was maintained in the dark at room temperature. These cultures were then used as the source of inoculum for each 166-paper size applicable to the Chinese National Standard (CNS) A4 specification (210X 297 mm) 509722 Α7 B7 V. Description of the invention (164) Fermentation. Printed by the Consumers' Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the precautions on the back before filling this page)

Jm "Bacterial" flasks or cultures are inoculated by inoculating 2ml of oil-covered storage culture or by transferring a primary variant colony to a 175ml sterile medium in a 500ml three-throttle bottle sealed with Kaput Is also possible). Typically, after 16 hours of incubation on a cyclotron at 150 rpm, 28 QC, the strain culture is transferred into a generation bottle. The generation bottle is usually inoculated by adding 1% of the actively growing bacterial culture to a sterile 2% PP3 medium (for example, 2.0 ml per 175 ml of fresh medium). The broth was produced in a 500-ml three-throttle bottle covered with one Kaput. The resulting bottle was incubated on a cyclotron at 150 rpm, 28GC. Although successful fermentation is not limited to this length of time, the production fermentation is terminated after 24-72 hours. After proper incubation, the broth was poured into a sterile 1L polyethylene bottle, stirred at 2600xg for 1 hour at 10oC and decanted to separate from the cells and debris sediment. Further clarification of the broth was accomplished by using a 0.5 μM pipe open-type polyethersulfonium (PES) filter to flow to a microfiltration device (Pal 1 Filtron, Northborough, MA). The resulting broth was then made using a 10,000 or 100,000 MW blocking membrane, an M12 ultrafiltration device (Amicon, Beverly, MA) or a centrifugal concentrator (Millipore, Bedford, MA) with a pore size of 10,000 or 100,000 MW. and Pall Filtron, Northborough, MA) were concentrated (up to 10 times). In the example of a centrifugal concentrator, the broth was centrifuged at 200xg for about 2 hours. The membrane permeate is added to the corresponding retentate to achieve a desired component concentration greater than the pore size used. Following these procedures, the broth was used for biochemical analysis or filtered and sterilized by a 0.2 μM nitrocellulose membrane filter for biology-167- This paper is sized to the Chinese National Standard (CNS) A4 specification (210 × 297) (Mm) Printed by the Consumer Cooperatives of the Central Bureau of Standards of the Ministry of Economic Affairs 509722 A7-: -----B7 V. Evaluation of Invention (165) " a " '-" The heating inactivation of the processed broth samples was achieved by heating the samples at 1000 ° C for 10 minutes by using a heating pad filled with sand. These broth and toxin complexes derived from different strains of Coryneform bacteria are useful for reducing insect populations and are used in a method of inhibiting an insect population, which comprises applying an effective insect inactive amount of the active substance In an area of the insect. The breadth of insecticidal activity observed in broths fermented by a group of selected Bacillus strains as described above is shown in Table 36. Improved or additional insecticidal activity may be measured in these strains by increasing the concentration of the broth or applying different fermentation methods. Consistent with the activity related to a protein, the insecticidal activity of all tested strains was apyrogenic. Cultured broths from different strains of Corynebacterium showed different insecticidal activity (mortality and / or growth inhibition) against a variety of insects. More specifically, this activity is seen against corn rootworms, which is a member of the insect Coleoptera. Other members of the Coleoptera include cotton weevil, caterpillar, pollen beetle, flea beetle, seed tapeworm and Colorado potato tapeworm. These broths and purified toxin complexes are also active against tobacco budworms, tobacco hornworms, and European corn perforators, and they are members of the order Lepidoptera. Other members of this order are beet armyworm, Korean cabbage moth, brown sugar moth, corn cotton fluke, apple moth, tapeworm, Indian rice moth, leaf roller, cabbage bug, cotton moth, sleeping bagworm, oriental tent caterpillar , Lawn networms and autumn burglars. The activity is also seen against German cockroaches, which is a member of the order Homoptera (or Salamander). Other members of this order are Oriental Turnworm and American Weevil. -168- This paper size applies to Chinese national standards (CNS> A4 size (21〇 '/ 297 mm) (Please read the precautions on the back before filling out this page) One Pack-Order 509722 A7 ____._ B7 V. Description of the Invention (I66) The activity against corn rootworm larvae was tested as follows: Bacillus culture broth (10-fold concentrated, filter sterilized), 2% protein glycoprotein gland No. 3 (10-fold concentrated), purified toxin complex, 10 mM sodium phosphate buffer (ρΗ7 · 0) was directly applied to the surface of artificial feed (approximately 1.5 cm2) in 40 μ1 aliquots (Rose, RI and McCabe, JM 1973. J. Econ. Entomol. 66, 398-400 ). The toxin complex was diluted with 10 mM sodium phosphate buffer (ρΗ7.0 ·). The feed tray was air-dried in a sterile operation box, and the wells were put into undeci. · (Southern Jade Plate Gas, SCR). These trays are sealed, placed in a humid growth box and maintained at 27QC for an appropriate period of time (3-5 days). Mortality and larval weight are then recorded. Usually, all Sixteen insects were used for each treatment in the study. The control group died The rate is less than 5%. Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (please read the precautions on the back before filling this page). The activity against lepidoptera larvae was tested as follows. Control medium (2% No. 3 proteoglycan peptone), purified toxin complex, 10 mM sodium phosphate buffer (ρΗ7.0 · 0) were directly applied to standard artificial lepidopteran feed (Stoneviue Yeii〇w feed) in 40 μ1 aliquots. ) Surface. These feed trays are placed in a sterile extraction cabinet and allowed to air dry, and each well is placed into a single, newly born larva. The European corn perforator iOstrinia nubilalis) and tobacco specialist Qjlfanduca sexta) are unloaded. Obtained from commercial sources and hatched indoors. Tobacco buds (valves / pupae / no larvae are self-supplied. After placing the larvae, these feed trays are sealed, placed in a humidified growth box and kept dark And 27 ° C for an appropriate period of time. Mortality and weight measurements were recorded on day 5. Generally, 16 insects were used per treatment in all studies. Control group mortality range -169- Use China National Standard (CNS) A4 specifications (2.0 × 297 public directors) " 509722 A7 B7 V. Description of the invention (167) Usually in the control medium between about 0 to about 12.5%, in phosphate buffered saline It is less than 10%. The activity against cockroaches was tested as follows. Concentrated (10 times) bacilliform broth culture broth and control medium (2% No. 3 protein glycoprotein gland) were directly applied to the standard in 40 μl aliquots. The surface (about 1.5 cm2) of artificial lepidopteran feed (Stonevi 1 le Yellow feed). These feed trays were placed in a sterile suction cabinet and allowed to air dry, and placed into a single, 1-year-old German cockroach (Cang / a) anesthetized by C02. After placement, these feed trays were sealed and placed in a humidified The growth box was kept in the dark for an appropriate period of 27 GC. Mortality and weight were recorded on the 5th day. The mortality rate in the control group was less than 10%. Table 36 Broth derived from different strains of Bacillus Observed insecticidal spectrum of strains of Bacillus spp. Sensitive to insect species Ύ. Zealandica P. hepialus HB-Arg HB Oswego HB Lewiston K-122 HMGD Indicus

(Please read the notes on the back before filling this page) Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs GD PWH-5 Megidis HF-85 A. Cows MP1 MP2 MP3 MP4 MP5 GL98 GL101 GL138 GL155 GL217 GL257 DEP1 DEP2 DEP3 420 ^ 2422 4n40i44c 3 This paper size applies Chinese National Standard (CNS) A4 specification (210X 297 mm) • 170- 509722 A7 ___B7_ V. Description of the invention (168) Compared with the control group, the mortality and / or growth inhibition is greater than or equal to 25% . : Tobacco budworm; 2: European corn perforator; 3: Tobacco hornworm; 4: Southern jade water rootworm; 5. German township. (Please read the precautions on the back before filling this page) 歹 歹! 24_ 利 J W-14 盖 _1 后 ^ 的 non w-14 bare rod-shaped _ _ Zi Xi Qugufen refracted rod-shaped strains grow in 2% of No. 3 protein glycoprotein gland agar (Difco Laboratories, Detroit, MI), insecticidal toxin ability was maintained through repeated biological analysis after generation. A 50 ml shake culture was produced in 2% No. 3 protein glycoprotein gland medium in a 175 ml throttling bottle, which was grown at 28 GC at 150 rpm for approximately 24 hours. 15 ml of this culture was centrifuged (700xg, 30 minutes) and frozen in its culture medium at -20GC until thawed (slowly in ice water) for DNA isolation. The lysed W-14 culture was centrifuged (900 xg, 15 minutes, 4QC) and the floating orange mucopolysaccharide material was removed. The remaining cellular material was centrifuged (25,000 OOOg, 4GC) to precipitate the bacterial cells, and the medium was removed and discarded. Printed by the Consumer Standards Cooperative of the Central Bureau of Standards of the Ministry of Economics The aggregate DNA was separated by a modification of the CTAB method as described in Section 2.4.1 of Ausubelei a / · (1994). The modification included a high salt impact, and all volumes were increased by a factor of 10 over the recommended volume for the "microprecipitation". Unless otherwise specified, all centrifugations were performed at 4GC. The precipitated bacterial cells were resuspended in TE buffer (10 mM Tris-HCl, ImMEDTA, pH 8.0) to a final volume of 10 ml, followed by addition of 12 ml of 5M NaCl; the mixture was centrifuged at 15, OOOxg for 20 minutes. The precipitate was resuspended in 5.7 ml TE and 300 μ1 of 10% SDS and 60 μ1 of 20 mg / ml proteinase K (dissolved in sterile distillation _-m-^ ^ ^ Applicable to China National Standard (匸 奶) 8 4 specifications (210 parent 297) (Mm) '' 509722 Printed by A7 B7, Consumer Cooperatives of the Central Bureau of Standards, Ministry of Economic Affairs 5. Description of the Invention (169) In water, Gibco BRL Products, Grand Island, NY) was added to the suspension. The mixture was incubated at 37 GC for 1 hour, and then about 10 mg of lysozyme (Worthington Biochemical Corp., Freehold, NJ) was added. After another 45 minutes of incubation, 1 ml of 5M NaCl and 800 μl of CTAB / NaCl solution (10% w / v CTAB, 0.7M NaCl) were added. This preparation was incubated at 65GC for 10 minutes, and then gently agitated; the incubation and agitation was performed again for about 20 minutes to assist the clarification of the cellular material. Add an equal volume of the gas imitation / isoamyl alcohol solution (24: 1, v: v) and mix very gently. The phases are separated by centrifugation at 12, OOOxg for 15 minutes. The upper (aqueous) phase was removed with a wide-mouth pipette, and then an equal volume of PCI (phenol / aeroform / isoamyl alcohol, 50: 49: 1, v: v: v; 1M Tris-HC1 , Ρ 之 8.0 · Equilibrium; Intermountain Scientific Corporation, Kaysville, UT) Extraction was performed twice as above. The DNA was precipitated with 0.6-fold volume of isoamyl alcohol, and transferred to a glass rod, washed twice with 70% ethanol, dried and dissolved in 2 ml of STE (10mM Tris-HC1, pH8.0; 10mM NaCl, ImM EDTA). The preparation contained 2.5 mg / ml DNA, which was quantified by an optical density of 260 nm. AgV hybridized with fc gene specific sting needles /// valence knife ^ / /// Fragment recognition of about 10 pg of genomic DNA at 7 // This // 30 units each with (NEB), which took 180 minutes to complete Digest, freeze overnight, then heat at 65QC for 5 minutes, and at 0.8 ° /. Agarose gel electrophoresis (Seakem® LE, IX TEA, 80 volts, 90 minutes). The DNA was stained with ethidium bromide (50 pg / ml) as described above, and photographed under ultraviolet light. The DNA fragments in the agarose gel are depurinated (5 minutes in 0.2M HC1), denatured (0.5M NaOH, -172-) This paper is in accordance with the Chinese National Standard (CNS) A4 specification (21〇 > < 297 mm) (Please read the notes on the back before filling this page) Order 509722 A7 B7 V. Description of the invention (170) 15M in 1.5M NaCl) and neutralization (0.5M Tris-HCl pH8.0, 1.5M NaCl for 15 minutes), dip 3 times with distilled water between each step. The DNA was transferred from the gel to a NYTRAN fortune membrane (Amersham, Arlington) using a high-salt (20X SSC) experimental procedure as described in section 2. 9 of Ausubel ei (CPMB as previously cited) by Southern transduction. Heights, IL). The transferred MA is then laterally connected to the fortune film with a setting of an automatic horizontally connected Stratagene UV Stratalinker. The films were stored dry at 25QC until use. Hybridization was performed using ECLTM direct (Amersham, Arlington Heights, IL) labeling and detection system in accordance with the experimental procedures provided by the manufacturer. Printed by the Consumer Standards Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (please read the notes on the back before filling this page). In short, the probe is prepared by covalently binding denatured DNA to the peroxidase enzyme . Once labeled, the probe is used under hybridization conditions that maintain the enzyme activity. Unhybridized probes were removed by washing twice (20 minutes each) in 65GC, 0.5X SSC, 0.4% SDS, and 6M urea. It was then washed twice with 2X SSC at room temperature for 5 minutes each. As instructed by the manufacturer, ECLTM reagents were used to detect these hybridized DNA bands. There are several factors that can affect the ability to detect gene associations between different Bacillus strains and W-14 strains. First, high stringency conditions are not applied in such promiscuity. Changing the stringency of the hybridization and washing conditions will affect the pattern and intensity of the hybridization band, which is known in the art. Secondly, the difference between the turnaround and turnover in the south will affect the mobility and molecular weight estimates of the hybrid zone. As a result, W-14 was included as a standard for all southern turns. Gene-specific probes derived from the W-14 toxin gene are used for these -173- This paper size applies Chinese National Standard (CNS) A4 specifications (210 '〆297 mm) 509722 A7 B7 V. Description of the invention ( m, in hybridization. The following are the specific equivalents of each gene sequence corresponding to the probe. One of the specificity probes: sequence identification number 25 1174 to 3642; for fcaC One of the probes with specificity: 3637 to 6005 of sequence identification number 25; one of the probes with specificity to icad · 2097 to 4964 of sequence identification number 11 and the probe with specificity to chemistry Needle ·· Sequence ID No. 46-1660 to 4191. The following tables summarize the analysis of the southern turn of the Bacillus strains. In the case of hybridization of the probe, the hybridized fragments are recorded as the The observed spectra of -14 strains are the same or different. Table 37 Southern analysis of Bacillus spp. (Please read the precautions on the back before filling out this page) tcdA tcbA tcaC tcaB / Bn WX-1 DDDD WX- 2 DD — D WX-3 DDDD WX-4 Γ DD ND D WX-5 DDDD WX-6 Fd DDD WX-7 DD ND D WX-8 DDDD WX-9 ND DDD WX-10 ND DDD WX-11 ND DDD WX -12 DDDD WX-14 DDDD WX-15 DDDD HP88 D — DD Hm D — DD Hb D — D — H9 D — ID B2 D — D — NC-1 D-DD WIR D — DD W30 DDDDW -14 IIII -174 . This paper size applies Chinese National Standard (CNS) A4 (210X297 mm) 509722 A7 B7 V. Description of the invention (172) ND = Not determined;-= No measurable hybrid product; 1 = Same fragment map; D = Different fragment maps. ~ Southern analysis of the disease

Strain tcdA tcbA tcaC tcaBf / Bi; K-122 3. 3, 2. 8 D ND PWH-5 + DD Indicus DD 3. 0 I Megidis DDD GD DDD HF-85 DDD-MP 3 D-D-MP 1 D + D-A. Cows D + D-HB-Arg D ND D-HMGD DDD-HB Lewiston DDD-HB Oswego DDD-W -14 IIII ND = not determined;-= = no measurable hybrid product; 1 = same Fragment map; different fragment maps; + = unmeasured hybrid fragment map. Table 39 Southern analysis of Bacillus mycoides (please read the precautions on the back before filling out this page) i Binding and ordering strains tcdA tcbA tcaC tcaB; D ϋτππ + + D ϋ1381 + D-1-1 Γ ^ Τ7 + 1 t) UT2bV + + D MF3 1 + 1 MP5 1-— P · hevialus + 1 D Ρ. Zeal andica +-1 · 0 MP1 DDD pin 2 DD ϋ 1) EP3 DDDW ^ 14 3 · 8, 2 ^ 1 2. δ 2. 8 -175- This paper size is applicable to China National Standard (CNS) A4 (210X297 mm) 509722 Employees' Cooperatives of Central Standards Bureau, Ministry of Economic Affairs Print A7 B7 V. Description of the invention (173) ND = Not determined;-= No measurable hybrid product; 1 = Same fragment map; D = Different fragment map; + = Untested hybrid fragment map. In these analyses, it is clear that the homogeneous substance of the W-14 gene is distributed in these Corynebacterium strains, as evidenced by the difference in the size of the gene fragments between W-14 and other strains. Example 25 N-terminal amino acid peptide of toxin complex peptide The peptide relationship (as described in Example 14) of different P. coryneform strains was sequenced by N-terminal amino acids. The N-terminal amino acid sequences of the toxin peptides in multiple strains were compared with Comparison of W-14 toxin peptides. In Table 40, comparison with one of the data comparisons shows that the toxin peptides are the same or homogeneous (at least 40% similarity to the W-14 gene / peptide). The peptide is present in all of these strains. For example, the N-terminal amino acid sequence (sequence identification number 2) of TcaC was found to be the same as that of the 160kDa peptide in HP88, but homogeneous substances were also present in WIR, H9 , Hb, WX-1 and Hm strains. Some W-14 peptides or homogenates were not found in other strains; however, due to the N-terminal blockage or low content, not all wins were derived from Sequences of toxin complexes from other strains. In addition, many other N-terminal amino acid sequences (sequence identification numbers 82 to 88) have been obtained for toxin complex peptides from other strains, such as The toxin complex peptide is not similar to the peptide from W-14 and is identical to each other in some cases For example, an identical amino acid sequence sequence identification number 82 is obtained from a 64 kDa version of the HP88 and Hb strains, and a homogeneous sequence is obtained from a 70 kDa peptide from the M-ΐ strains ( Sequence identification number 83). -176- This paper size applies to Chinese National Standard (CNS) A4 (210 X 297 mm) "-(Please read the precautions on the back before filling this page)

1T d 509722 A7 B7 V. Explanation of the invention (174) Table 40 Comparison of amino group sequence homogeneity between proteins isolated from non-W-14 strains. W-14 peptide printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs. ff-14 gene ff-14 sequence identification strains identical homology sequence identification number TcaAii tcaA 15 TcaAiU tcaA 4 TcaBi tcaB 3 76 H9-74 kDa 76 Hm-71 kDa TcsBjj tcaB 5 H9 61 kda-Hm 61 kda-TcaC tcaA 2 72 Hb-160 kDa HP88 160 kDa-73 WIP-170 kDa 74 H9-180 kDa 75 Hm-170 kDa 80 WX-1-170 kDa TcbAji tcbA 1 TcbAiii tcbA 40 TccA tccA 8 77 Hb-81 kDa TccB tccB 7 ffX-1 170 kDa-WX-2 180 kDa-ffX-14 180 kDa-WIR 170 kDa-78 H9-170 kDa NC-l 140 kDa-79 Hm-190 kDa TcdA "tcdA TcdAjji tcdA 41 Hb 57 kDa-81 H9-69 kDa ?? 9 Hb 86 kDa-HP88 86 kDa 1-Homogeneity refers to an amino acid sequence with at least 40¾ similarity to the W-14 gene / peptide. Similar residues are identified as the following five One of the members: (P, A, G, S, T), (Q, N, E, B, D, Z), 〇1,1 (, 1〇, (1,1,1 ^ 〇 and (? , 11). __ (Please read the precautions on the back before filling out this page) One pack · 4 Example 26 Immunological analysis of Bacillus strains The culture broth of Bacillus strains uses a Centriprep-IO ultrafiltration device (Amicon , Inc. Beverly, MA 01915) is concentrated 10 to 15 times. -177- This paper size applies the Chinese National Standard (CNS) A4 specification (210X297 mm). Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs 509722 A7 _B7 _._ 5. Description of the invention (I75) The concentration of the protein ranges from 0.3 to 3.0 mg / ml. Total protein 10 to 20 pg was packed into a well of 4-20% polypropylene coagulation gel (Integrated Separation Systems, Natick, Μ01 01760) in each well of 0 gel electrophoresis using a constant setting of 25 mA per gel The current was carried out for L for 25 hours. The gel was electroconverted to a Hybond-ECLT * ^ acid cellulose membrane (Amersham Corporation, Arlington Hts, IL 60005) using a semi-dry electrorotator (Pharmacia Biotech Inc., Piscataway, NJ 08854). 5 小时。 A stable current was supplied at 0.75mA / cm, which lasted 2.5 hours. The membrane was blocked with 10% milk in TBST (25 mM Tris-HCl, pH 7.4, 136 mM NaCl, 2.7 mM KC1, 0.1% Tween 20) for 1 hour at room temperature. Each-grade antibody was diluted to 1: 500 with 10% milk / TBST. Other dilutions between 1:50 and 1: 1000 are also used. The membrane was incubated in a primary antibody for at least 1 hour. It was then washed thoroughly with blocking solution or TBST. A secondary antibody (goat anti-mouse IgG or goat anti-rabbit IgG that binds to peroxidase) was dissolved in 10% milk / TBST at a 1: 2000 dilution. BioRad Laboratories, Hercules, CA 94547 was added to a The membrane on the platform rocker lasted 1 hour. The film was then washed with a large amount of TBST. The detection of this protein was performed using an ECL (Promoting Chemiluminescence) Detection Kit (Amersham International) to generate 0-peptide-specific antibodies specific to W-14 peptides. The chart was used to confer using Western turn analysis. Characteristics of this protein composition in broth from 9 non-W-14 strains of Corynebacterium. In addition, a single-source antibody (Mab-C5F2) capable of recognizing the TcbAHi protein in the W-14-derived toxin complex was used. These results (Table 39) show resistance to certain proteins in these broths _____- 178- _ This paper size applies to China National Standard (CNS) A4 specifications (210X297 mm) " (Please read the note on the back first Please fill in this page for matters) | Binding · Order 509722 A7 B7 V. Cross-recognition of the antibody of the invention (176). In some examples, the proteins identified by the proteins are the same size as the W-14 target peptide. In other examples, the proteins identified by the proteins are smaller than the w-14 target peptide. This data shows that some of these non-W-14 coryneform strains can produce proteins similar to the W-14 strain. This difference may be due to deletion or protein processing or degradation processing. Some of these strains do not contain proteins that can be recognized by certain antibodies; however, this concentration may be much lower than the observed W-14 peptide. When different toxin peptide homogenates are compared 'these results show peptide ambiguity among these Bacillus strains. ΑΛ1__Cross-recognition of Bacillus strain Mab C5F2 PAb TcdAu of Bacillus sp syn PAb TcdAiU -syn PAb TcaC -syn PAb TcaBii -syn PAb TcbAUi -syn PAb TcaBi-syn PAb TcaAii -syn PAb TcaAiu -syn MP1-+ + +-+ + + + MP2 + + + +-+ + + + MP3 One + + +-NT + +-A. Cows One + + +-NT + + + Hb-osw One-NT + + NT + + + H-Arg-+ +--NT + + + Hb-leu-+ + +-NT + + + Indicus + + + + + NT + + + HF85-+ + +-+ + + + W-14 + + + + + + + + + +: positive reaction;-: negative reaction; NT : Not tested. Other W-14 coryneform bacteria strains were characterized by using the Western Blast analysis to use the culture broth and / or partially purified protein isolates as antigens. The antibody's chart includes Mab-C5F2, Mab-DEl (recognizes TcdAd, PAb-DE2 (recognizes TcaB), PAb_TcbAu-syn, PAb- -179-) This paper size applies to the Chinese National Standard (CNS) A4 specification (210X297) 1 ^ ------- 0 ^! (Please read the notes on the back before filling out this page) Order d Printed by the Employees 'Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs Printed by the Employees' Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 509722 A7 B7 V. Invention Description (I77)

TcaC-syn, PAb-TcaBH-syn, PAb-TcbAiU-syn, PAb-

TcaBi-syn. These antibodies showed cross-reactivity with proteins in the broth and in the fractions of non-W-14 strains. This data shows that antibodies can be used to identify proteins in the broth that are separated from the partially purified proteins. Table 41 Cross-recognition of Bacillus sp MAb Mab PAb PAb PAb PAb PAb PAb C5F2 DEI DE2 TcbAjj TcaC TcsBji TcbAHi TcaBj -syn -syn -syn -syn -syn WX -1 + + + + + + + WX-2 + + + + + + NT + WX-3 + NT + NT NT NT NT NT WX-5 + NT + NT NT NT NT NT WX-6 + NT NT NT NT NT NT NT NT WX-7 + + + + + + NT + WX- 8 + NT NT NT NT NT NT NT ffX-9 + NT NT NT NT NT NT NT WX-10-NT NT NT NT NT NT NT ffX-12 + + + + + + + + ffX-14 + + + NT + NT + WX -15 + NT NT NT NT NT NT NT NT W30 + + + NT NT NT NT NT Hb-NT + NT + NT One + H9--+ NT + + NT NT Hm-NT + + + + NT + + HP88-NT +-+ one-+ NC-1 +-+ + + + NT + WIR-NT + + + + + + W-14 + + + + + + + +-: negative reaction; +: positive reaction ; NT: Not tested. -180- This paper size is in accordance with Chinese National Standard (CNS) Α4 size (210 X 297 mm)-_ ϋ-ι_-1-«HI t_m —ϋ ϋ (Please read the precautions on the back before filling this page) Order 509722 A7 B7 V. Description of the Invention (178) Example 27 The bacterial manifestation of the coding region M of the heart is the performance of the certain bacterium, and the 5 'and 3' of the coding region (sequence identification number 46) The ends are modified to increase the available colony sites to embed the fragment in a heterologous expression vector. The ends were modified using unique primers in a polymerase chain reaction (PCR), which were performed essentially as described in Example 8. Primer sets (described below) are used in conjunction with agglomerates 21D2.4 as templates to produce products with such suitable modified ends. The first primer set was used to modify the 5 'end of the gene. The forward primer A0F1 (5' GAT CGA TCG ATC CAT GGC CAA CGA GTC TGT AAA AG A GAT ACC TGA TG TAT TAA AAA GCC AGT GTG 35 ) Embed a unique valence σ / address in the start codon and use the reverse primer A0R1 (5, GAT CGA TCG TAC GCG GCC GCT CGA TCG ATC GTC GAC CCA TTG ATT TGA GAT CTG GGC GGC GGG TAT CCA GAT AAT AAA CGG AGT CAC 3)) Add your unique 7 //, 5W / value i / address to make the embedding of the remaining part of the gene easier. Another PCR reaction is designed to modify the 3 'end of the gene by adding an extra stop codon and a convenient restriction site for selection. This is printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (please read the precautions on the back before filling this page). Introducer A0F2 (5 'ACT GGC TGC GTG GTC GAC TGG CGG CGA TTT ACT 3') is used for amplification A unique / site is crossed, which is then used to breed the 3 'end of the modification. The reverse primer A0R2 (5 'CGA TGC ATG CTG CGG CCG CAG GCC TTC CTC GAG TCA TTA TTT AAT GGT GTA GCG AAT ATG CAA AAT 3') was used to embed a second stop codon and /, and 朐 ί / Selection site. Bacterial expression vector pET27b (Novagen, -181- This paper size applies to Chinese National Standard (CNS) A4 specifications (210X: Z97 mm) Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 509722 Α7 ____ Β7 ___ V. Description of invention (Μ)

(Madison, WI) was modified according to standard molecular biology techniques to delete the address 7 // at position 446. The 497bp PCR product from the first amplification reaction (A0F1 + A0R1, which modified the 5 'end of the gene) was ligated into the modified pET27b vector according to the supplier's instructions. The DNA sequences of the amplified portions of the 3 isolates were determined using primers recommended by the supplier and the sequencing method described previously. This sequence is the same for all isolates. An isolate was then used as a breeding vector to embed the intermediate part of the gene in a 6341bp set 7 // to / fragment. The obtained strain is called MC4, and it contains all the chemistries to encode a region except the 3 'portion. Finally, in order to complete the full-length coding region, the 832bp PCR product from the second amplification reaction (A0F2 + A0R2, which modifies the 3 'end of the gene) is linked to the on // to according to standard molecular biology techniques MC4 isolate on ribs / fragment. The centered coding region was sequenced and found to be complete. The resulting plastid was called PDAB2035. Construction of pDAB2036, PDAB2037 and t) DAB2038 plastids expressed by icW bacteria. The icdi coding region was cut out with restriction enzyme claws and / or from pDAB2035 plastids and gel purified. This fragment was linked to the price σ / and / Λσ / of the PET15 expression vector to make pDAB2036 plastid. In addition, PDAB2035 is cut at valence σ / and Abi / to release the ici / J coding region, which is connected to the valence σ / and Abi / of the pET28b expression vector to make a PDAB2037 plastid. Finally, the plastid pDAB2035 is cut with a value of sigma / and / to release the coding region. This snippet is connected to ///-cut-182- This paper size applies to the Chinese National Standard (CNS) Α4 specification (210X 297 mm) (Please read the precautions on the back before filling this page), a st »d Economy Printed by the Consumer Standards Cooperative of the Ministry of Standards, Ministry of Standards, China 509722 A7 __B7 V. Description of the Invention (⑽) Cut, then use T4 DNA polymerase to blunt the ends of these trc99a expression vectors. This vector is then cut at valence ο / cut and ligated to a fragment of valence σ // 5Yw / cut. The resulting plastid is called PDAB2038 plastid. Emergence of PDAB2038 plastids PDAB2038 plastids were transferred into BL21 cells to transform them and behaved as described previously in pDAB2033 in Example 19. Purified from E. coli The performance culture was centrifuged at 10,300 xg for 30 minutes and the supernatant was collected. It was diluted with 2 volumes of water and added to a Poros 50 HQ (PerSeptive Systems, ΜΑ) column pre-equilibrated with 10 mM sodium phosphate buffer pH 7.0 (A buffer) at a flow rate of 7.5 ml / min ( 1.6cm X 10cm). The column was washed with A buffer until the optical density returned to the baseline level at 280 nm. The proteins bound to the column were then eluted with 1M NaCl in A buffer. This separation portion was packed into a gel filtration column in 20 ml aliquots, which was Speharose CL-4B (2.6 X 100 cm) equilibrated with A buffer. The protein was eluted in A buffer at a flow rate of 0.75 ml / min. Separation fractions with retention times between 260 and 460 minutes were pooled and added at 1 ml / min to a Mono Q 5/5 column equilibrated with 20 mM Tris-HC1, ρΗ7 · 0 (B buffer). The column was washed with B buffer until the optical density returned to baseline at 280 nm. The proteins bound to the column were eluted at 1 ml / min in a linear gradient of 0 to 1M NaCl in one of B buffers for 30 minutes. One milliliters of separated fractions were collected, serially diluted, and subjected to biological analysis. The separated part eluted between 〇 · 1 and 〇3M-183- This paper size applies to Chinese National Standard (CNS) A4 specification (210X297 mm) (Please read the precautions on the back before filling this page) 509722 A7 B7 V. Description of the Invention (181) It was found to have the highest insecticidal activity. Western analysis using the active isolated portion of pAb TcdAH-syn antibody and pAb TcdAiii-syn antibody revealed the presence of TcdAu and TcdAni peptides. ------- install-(Please read the precautions on the back before filling this page)

^ T d. Printed by the Consumer Cooperatives of the Central Bureau of Standards of the Ministry of Economic Affairs 84 11 This paper size applies to China National Standard (CNS) A4 (210 X 297 mm) 509722 A7 B7 V. Description of the invention (182) Sequence list ⑴ Basic data

(i) Applicant: Ensign, Jerald C

Bowen, David J Petell, James Fatig, Raymond Schoonover, Sue ffrench-Constant, Richard Orr, Gregory L Merlo, Donid J Roberts, Jean L Rocheleau, Thomas A (ii) Problem of the invention: insecticidal properties derived from light rod bacteria Protein toxin (iii) Total number of sequences: 88 Mailing address: (Α) To: DowElanco (B) Street: 9330 Zionsville Road (C) City: Indianapolis (D) State: Indiana (E) Country: United States ( F) Postal code: 46268 (v) Readable computer type: (A) Media type: floppy disk (B) Computer: IBM PC compatible model

(C) Operating system: PC-D〇S / MS-D〇S (D) Body: batentln Release # 1.0, Version # 1.30 (vi) Application information: (A) Application number: (B) Date of filing: (o category · (vii) past applications * materials: (A) application number: US 08 / 063,615 printed by the Staff Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (please read the notes on the back before filling in this (Page) (B) Date of filing: May 18, 1993 (vii) Information on past applications: (A) Application number: US 08 / 395,497 (B) Date of filing: February 28, 1995 (vii) Past application information: (A) Application number: US 60 / 007,255 (B) Filing date: November 06, 1995 (vii) Past application information: (A) Application number: US 08 / 608,423 ( B) Date of filing: February 28, 1996 (vii) Past application materials: (A) Application number: US 08 / 705,484 This paper size is applicable to Chinese National Standard (CNS) A4 specification (210X) l% ^ (%) 509722 A7 B7 V. Description of the invention (183) (B) Application date: August 28, 1996 (vii) Past application information: (A) Application number: US 08 / 743,699 (B) Application date : November 06, 1996 (viii) Law Division / Agent Information: (A) Name: Borucki, Andrea T. (B) Registration Number: 33651

(C) Reference / File No .: 50301E (ix) Contact information: (A) Phone: 317-337-4846 (B) Fax: 317-337-4847 ⑵Serial identification number No. 1: Information: (i) Sequence characteristics : (A) Length: 11 amino acids (B) Type: Amino acid (C) Number of strands: (D) Form: Linear (ii) Molecular type: Protein ⑺ Fragment type: N-terminal (xi) Sequence description 'Serial identification number 1 (TcbAn N-terminal):

Phe He Gin Gly Tyr Ser Asp Leu Phe Gly Asn 1 5 10 资料 Data of sequence identification number No. 2: (i) Sequence characteristics: (A) Length: 12 amino acids (B) Type: amino acid (C ) Number of shares: (D) Form: Linear (ii) Molecular type: Protein (v) Fragment type: Printed by the Consumer Cooperative of the Central Standards Bureau of the N-terminal Ministry of Economic Affairs (Please read the precautions on the back before filling this page) ( xi) Sequence description ^ • Sequence identification number 2 (TcaC N-terminal): Met Gin Asp Ser Pro Glu Val Ser He Thr Thr Trp 1 5 10 资料 Sequence identification number 3: (i) Sequence characteristics: (A) Length: 19 amino acids (B) Type: The size of the amino acid paper is applicable to the Chinese National Standard (CNS) A4 specification (210X2971 ^) 509722 A7 B7 V. Description of the invention (184) (ii) Molecular type: Peptide fragment type: N-terminal (xi) sequence: 'Sequence identification number 3 (TcaBi N-terminal):

Ser Glu Ser Leu Phe Thr Gin Thr Leu Lys Glu Ala Arg Arg Asp Ala 15 10 15

Information of Leu Val Ala ⑵Serial Identification Number 4: (i) Sequence characteristics: (A) Length: 14 amino acids (B) Type: amino acids (C) Number of strands: (D) Form: linear ( ii) Molecular type: protein (v) fragment type: N-terminal (xi) sequence description: sequence identification number 4 (TcaAin N-terminal):

Ala Ser Pro Leu Ser Thr Ser Glu Leu Thr Ser Lys Leu Asn 1 5 10 (2) Information of sequence identification number 5: (i) Sequence characteristics: (A) Length: 9 amino acids (B) Type: Amino acid (C) Number of strands: (D) Form: Linear (ii) Molecular type: Protein (v) Fragment type: N-terminus (xi # 列 列 ii: Sequence identification number 5 (TcaBi i N-terminus) ): Printed by the Consumer Standards Department of the Central Bureau of Standards of the Ministry of Economic Affairs (Please read the notes on the back before filling this page)

Ala Gly Asp Thr Ala Asn He Gly Asp 1 5 资料 Data of Sequence Identification Number 6: (i) Sequence Features: (A) Length: 15 Amino Acids (B) Type: Amino Acids (C) Number of Shares : Single strand (D) Morphology: Linear (ii) Molecular type: Protein M fragment type: N-terminal (xi) Sequence description: Sequence identification number No. 6: This paper size applies Chinese National Standard (CNS) A4 specification (210X 297 mm) 509722 A7 B7 V. Description of the invention (l85)

Leu Gly Gly Ala Ala Thr Leu Leu Asp Leu Leu Leu Pro Gin lie 1 5 10 15 资料 Sequence ID No. 7: (i) Sequence characteristics: (A) Length: 11 amino acids (B) Type: Amino acid (C) Number of strands: (D) Form: linear (ii) Molecular type: protein (0-fragment type: N-terminus (xi) Sequence description: Sequence identification number 7 (TccB N-terminus):

Met Leu Ser Thr Met Glu Lys Gin Leu Asn Glu 1 5 10 资料 Data of sequence identification number 8: (i) Sequence characteristics: (A) Length: 9 amino acids (B) Type: amino acid (C ) Number of strands: (D) Form: Linear (ii) Molecular type: Protein (v) Fragment type: N-terminal (xi) Sequence description: Sequence identification number 8 (TccA N-terminal):

Met Asn Leu Ala Ser Pro Leu He Ser 1 5 资料 Serial Identification Number No. 9: Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (please read the precautions on the back before filling this page) (i) Sequence characteristics: (A) Length: 16 amino acids (B) Type: Amino acid (C) Number of strands: (D) Form: Linear (ii) Molecular type: Protein (v) Fragment type: N-terminal (xi) sequence Description: Serial identification number 9: 2gg This paper size is applicable to Chinese National Standard (CNS) A4 specification (210X297 mm) 509722 A7 B7 V. Description of the invention (l86)

Met lie Asn Leu Asp He Asn Glu Gin Asn Lys lie Met Val Val Ser 15 10 15 资料 Sequence identification number 10: (i) Sequence characteristics: (A) Length: 20 amino acids (B) Type: Amino acid (C) Number of strands: (D) Form: linear (ii) Molecular type: protein (v > fragment type: N-terminal (xi) sequence description \ Sequence identification number 10:

Ala Ala Lys Asp Val Lys Phe Gly Ser Asp Ala Arg Val Lys Met Leu 15 10 15

Arg Gly Val Asn 20 ⑵Data of sequence identification number 11: (i) Sequence characteristics: (A) Length: 7515 base pairs (B) Type: Nucleic acid (C) Number of strands: Double strand (D) Form: Linear (ii) Molecular type: DNA (genome) (ix) Characteristics:

(A) Name / Tips: CDS (B) Location: 1..7515 (xi) Sequence description: Sequence identification number 11 (tcbA gene): Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (please read the first Please fill in this page again) ATG CM MC TCA m TCA AGC ACT ATC GAT ACT ATT TGT CAG AAA CTC 48

Met Gin Asn Ser Leu Ser Ser Thr He Asp Thr He Cys Gin Lys Leu 15 10 15 CAA ΤΓΑ ACT TGT CCG GCG GM Απ GCT TTG TAT CCC ΤΓΤ GAT ACT TTC 96

Gin Leu Thr Cys Pro Ala Glu lie Ala Leu Tyr Pro Phe Asp Thr Phe 20 25 30 CGG GM AAA ACT CGG GGA ATG GIT MT TGG GGG GM GCA AAA CGG ATT 144

Arg Glu Lys Thr Arg Gly Met Val Asn Trp Gly Glu Ala Lys Arg He 35 40 45 This paper size applies to Chinese National Standard (CNS) A4 (210X297 mm) 509722 Printed by the Consumers' Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs A7 B7 5 Description of the invention (187) TAT GAA ATT GCA CAA GCG GAA CAG GAT AGA AAC CTA CIT CAT GAA AAA 192

Tyr Glu lie Ala Gin Ala Glu Gin Asp Arg Asn Leu Leu His Glu Lys 50 55 60 OGT ATT ATT ΉΤ GCC TAT GCT AAT COG CTG CTG AAA MC GCT GTT OGG TTG 240

Arg He Phe Ala Tyr Ala Asn Pro Leu Leu Lys Asn Ala Val Arg Leu 65 70 75 80 GGT ACC CGG CAA ATC TTG GGT TTT ATA CM GGT TAT AGT GAT CTG TTT 288

Gly Thr Arg Gin Met Leu Gly Phe lie Gin Gly Tyr Ser Asp Leu Phe 85 90 95 GGT MT CGT GCT GAT MC TAT GCC GCG CCG GGC TCG GTT GCA TOG ATG 336

Gly Asn Arg Ala Asp Asn Tyr Ala Ala Pro Gly Ser Val Ala Ser Met 100 105 110 TTC TCA (XG GCG GCT TAT TTG ACG GAA TTG TAC ΟΪΤ GAA GCC AAA AAC 384

Phe Ser Pro Ala Ala Tyr Leu Thr Glu Leu Tyr Arg Glu Ala Lys Asn 115 120 125 TTG CAT GAC AGC AGC TCA Απ TAT TAC CTA GAT AAA CGT OGC (XG GAT 432

Leu His Asp Ser Ser Ser He Tyr Tyr Leu Asp Lys Arg Arg Pro Asp 130 135 140 m GCA AGC m ATG CTC AGC CAG AAA AAT ATG GAT GAG GAA Απ TCA 480

Leu Ala Ser Leu Met Leu Ser Gin Lys Asn Met Asp Glu Glu lie Ser 145 150 155 160 ACG CTG GCT CTC TCT MT GM TTG TGC CTT GCC GGG ATC GM ACA AAA 528

Thr Leu Ala Leu Ser Asn Glu Leu Cys Leu Ala Gly He Glu Thr Lys 165 170 175 ACA GGA AAA TCA CAA GAT GAA GTG ATG GAT ATG TTG TCA ACT TAT CGT 576

Thr Gly Lys Ser Gin Asp Glu Val Met Asp Met Leu Ser Thr Tyr Arg 180 185 190 TTA AGT GGA GAG ACA CCT TAT CAT CAC GCT TAT GAA ACT (jTT CGT GAA 624

Leu Ser Gly Glu Thr Pro Tyr His His Ala Tyr Glu Thr Val Arg Glu 195 200 205 ATC GTT CAT GAA CGT GAT CCA GGA ΉΤ CXjT CAT TTG TCA CAG GCA CCC 672 ---- aao, _ This paper size applies to Chinese national standards (CNS) A4 specification (210X 297 mm) 丨 ------- install-- (Please read the precautions on the back before filling this page) Order 509722 A7 B7 V. Description of the Invention (l88) lie Val His Glu Arg Asp Pro Gly Phe Arg His Leu Ser Gin Ala Pro 210 215 220 ATT GTT GCT GCT MG CTC GAT CCT GTG ACT TTG TTG GGT Απ AGC TCC 720 lie Val Ala Ala Lys Leu Asp Pro Val Thr Leu Leu Gly He Ser Ser 225 230 235 240 CAT ATT TCG CTA GAA CTG TAT AAC TTG CTG ATT GAG GAG ATC CCG GAA 768 His He Pro Pro Glu Leu Tyr Asn Leu Leu lie Glu Glu lie Pro Glu 245 250 255 AAA GAT GAA GCC GCG CIT GAT kCG CIT TAT AAA ACA AAC ΤΓΤ GGC GAT 816 Lys Asp Glu Ala Ala Leu Asp Thr Leu Tyr Lys Thr Asn Phe Gly Asp 260 265 270 ATT ACT ACT GCT CAG ΤΓΑ ATG TCC CXA AGT TGT OGG TAT TAT 864 lie Thr Thr Ala Gin Leu Met Ser Pro Ser Tyr Leu Ala Arg Tyr Tyr 275 280 285 GGC GTC TCA (XG GAA GAT Απ GCC TAC GTG ACG ACT TCA m TCA CAT 912 Gly Val Ser Pro Glu Asp lie Ala Tyr Val Thr Thr Ser Leu Ser His 290 295 300 GTT GGA TAT AGC AGT GAT ATT CTG GTT Απ CCG TTG GTC GAT GGT GTG 960 Val Gly Tyr Ser Ser Asp He Leu Val lie Pro Leu Val Asp Gly Val 305 310 315 320 GGT MG ATG GAA GTA GTT CGT GTT ACC CGA ACA CCA TCG GAT MT TAT 1008 Gly Lys Met Glu Val Val Arg Val Thr Arg Thr Pro Ser Asp Asn Tyr 325 330 335 ACC AC3T CAG ACG AAT TAT Απ GAG CTG TAT CCA CAG GGT GGC GAC AAT 1056 Thr Ser Gin Thr Asn Tyr lie Glu Leu Tyr Pro Gin Gly Gly Asp Asn 340 345 350 TAT TTG ATC AAA TAC AAT CTA AGC MT AGT m GGT TTG GAT GAT TTT 1104 Tyr Leu He Lys Tyr Asn Leu Ser Asn Ser Phe Gly Leu Asp Asp Phe 355 360 365 TAT CTG CM TAT AAA GAT GGT TCC GCT GAT TGG ACT GAG ATT GCC CAT 1152 Tyr Leu Gin Tyr Lys Asp Gly Ser Ala Asp Trp Thr Glu lie Ala His 370 375 380 (Please read the notes on the back side 1--then fill in. Write this Page) 4 _-1Q1-_ Paper scale applicable Chinese National Standard (CNS) A4 size (210X297 mm) 509722 A7 B7 V. description of the invention (l89) AAT CCC TAT (XT GAT ATG GTC ΑΤΑ AAT CM MG TAT GAA TCA CAG GCG 1200

Asn Pro Tyr Pro Asp Met Val He Asn Gin Lys Tyr Glu Ser Gin Ala 385 390 395 400 ACA ATC AAA CGT AGT GAC TCT GAC AAT ATA CTC AGT ATA GGG m CM 1248

Thr He Lys Arg Ser Asp Ser Asp Asn lie Leu Ser He Gly Leu Gin 405 410 415 AGA TGG CAT AGC GGT AGT TAT MT TTT GCC GCC GCC AAT TIT AAA ATT 1296

Arg Trp His Ser Gly Ser Tyr Asn Phe Ala Ala Ala Asn Phe Lys He 420 425 430 GAC CM TAC TCC CCG AAA GCTTTC CTG CIT AM ATG MT MG GCT ATT 1344

Asp Gin Tyr Ser Pro Lys Ala Phe Leu Leu Lys Met Asn Lys Ala lie 435 440 445 CGG TTG CTC MA GCT ACC GGC CTC TCT TTT GCT ACG TTG GAG CGT ATT 1392

Arg Leu Leu Lys Ala Thr Gly Leu Ser Phe Ala Thr Leu Glu Arg He 450 455 460 GTT GAT AGT GIT AAT AGC kCC AAA TCC ATC AOG GTT GAG GTA TTA AAC 1440

Val Asp Ser Val Asn Ser Thr Lys Ser He Thr Val Glu Val Leu Asn 465 470 475 480 MG GTT TAT CGG GTA AAA TTC TAT Απ GAT CGT TAT GGC ATC AGT GAA 1488

Lys Val Tyr Arg Val Lys Phe Tyr He Asp Arg Tyr Gly lie Ser Glu 485 490 495 GAG ACA GCC GCT ATT TTG GCT MT Απ MT ATC TCT CAG CM GCT GTT 1536

Glu Thr Ala Ala He Leu Ala Asn He Asn He Ser Gin Gin Ala Val 500 505 510 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling this page) GGC AAT CAG CTT AGC CAG TTT GAG CM CTA TTT AAT CAC CGG CCG CTC 1584

Gly Asn Gin Leu Ser Gin Phe Glu Gin Leu Phe Asn His Pro Pro Leu 515 520 525 AAT GGT ATT CGC TAT GAA ATC AGT GAG GAC MC TCC AAA CAT CTT CTT 1632

Asn Gly lie Arg Tyr Glu lie Ser Glu Asp Asn Ser Lys His Leu Pro 530 535 540 AAT CCT GAT CTG AAC CTT AAA CCA GAC AGT ACT GGT GAT GAT CAA CGC 1680 -m— This paper size applies to Chinese National Standard (CNS) A4 Specifications (210X297 mm) 509722 A7 B7 V. Description of the invention (190)

Asn Pro Asp Leu Asn Leu Lys Pro Asp Ser Thr Gly Asp Asp Gin Arg 545 550 555 560 MG GCG GTT ΤΓΑ AAA CGC GCG TTT CAG GTT MC GCC AGT GAG TTG TAT 1728

Lys Ala Val Leu Lys Arg Ala Phe Gin Val Asn Ala Ser Glu Leu Tyr 565 570 575 CAG ATG ΤΓΑ TTG ATC ACT GAT CGT AAA GM GAC GGT GTT ATC AAA MT 1776

Gin Met Leu Leu He Thr Asp Arg Lys Glu Asp Gly Val He Lys Asn 580 585 590 MC m GAG MT TTG TCT GAT CTG TAT TTG GTT AGT TTG CTG GCC CAG 1824

Asn Leu Glu Asn Leu Ser Asp Leu Tyr Leu Val Ser Leu Leu Ala Gin 595 600 605 ATT CAT MC CTG ACT ATT GCT GM TTG MC ATT TTG TTG GTG ATT TGT 1872 lie His Asn Leu Thr lie Ala Glu Leu Asn lie Leu Leu Val lie Cys 610 615 620 GGC TAT GGC GAC ACC MC ATT TAT CAG ΑΠ ACC GAC GAT MT m GCC 1920

Gly Tyr Gly Asp Thr Asn He Tyr Gin lie Thr Asp Asp Asn Leu Ala 625 630 635 640 AAA ΑΑΑ (ΠΌ GM ACA TTC TTG TGG ATC ACT CAA TGG TTG AAG ACC CAA 1968

Lys lie Val Glu Thr Leu Leu Trp He Thr Gin Trp Leu Lys Thr Gin 645 650 655 AAA TGG ACA GTT ACC GAC CTG TIT CTG ATG ACC ACG GCX: ACT TAC AGC 2016

Lys Trp Thr Val Thr Asp Leu Phe Leu Met Thr Thr Ala Thr Tyr Ser 660 665 670 ACC ACT ΤΓΑ ACG CCA GM Απ AGC MT CTG AOG GCT ACG TTG TCT TCA 2064 (Read the notes on the back and fill out this page)

Thr Thr Leu Thr Pro Glu lie Ser Asn Leu Thr Ala Thr Leu Ser Ser 675 680 685 ACT TTG CAT GGC AAA GAG AGT CTG ATT GGG GAA GAT CTG AAA AGA GCA 2112

Thr Leu His Gly Lys Glu Ser Leu lie Gly Glu Asp Leu Lys Arg Ala 690 695 700 ATG GCG CCT TGC TO ACT TCG GCTITG CAT ΉΌ ACT TCT CAA GAA GTT 2160

Met Ala Pro Cys Phe Thr Ser Ala Leu His Leu Thr Ser Gin Glu Val 705 710 715 720 This paper size applies to Chinese National Standard (CNS) A4 specification (210X 297 mm) 509722 A7 B7 V. Description of the invention (191) GCG TAT GAC CTG CTG TTG TGG ATA GAC CAG ATT CM CCG GCA CM ΑΤΑ 2208

Ala Tyr Asp Leu Leu Leu Trp lie Asp Gin He Gin Pro Ala Gin lie 725 730 735 ACT GIT GAT GO; TIT TGG GAA GAA GTG CAA ACA ACA CCA ACC AGC TTG 2256

Thr Val Asp Gly Phe Trp Glu Glu Val Gin Thr Thr Pro Thr Ser Leu 740 745 750 MG GTG ATT ACC ΊΤΓ GCT CAG GTG CTG GCA CM TTG AGC CTG ATC TAT 2304

Lys Val He Thr Phe Ala Gin Val Leu Ala Gin Leu Ser Leu He Tyr 755 760 765 CGT CGT Απ GGG m ACT GM ACG GM CTG TCA CTG ATC GTG ACT CM 2352

Arg Arg lie Gly Leu Ser Glu Thr Glu Leu Ser Leulie Val Thr Gin 770 775 780 TCT TCT CTG CTA GTG GCA GGC AAA AGC ATA CTG GAT CAC GGT CTG TTA 2400

Ser Ser Leu Leu Val Ala Gly Lys Ser He Leu Asp His Gly Leu Leu 785 790 795 800 ACC CTG ATG GCC TTG GAA GGT Ή CAT ACC TGG GTT MT GGC TTG GGG 2448

Thr Leu Met Ala Leu Glu Gly Phe His Thr Trp Val Asn Gly Leu Gly 805 810 815 CM CAT GCC TCC TTG ATA TTG GOG GCG TKJ AAA GAC GGA GOC TTG ACA 2496

Gin His Ala Ser Leu He Leu Ala Ala Leu Lys Asp Gly Ala Leu Thr 820 825 830 GTT ACC GAT GTA GCA CAA GCT ATG AAT AAG GAG GAA TCT CTC CTA CAA 2544

Val Thr Asp Val Ala Gin Ala Met Asn Lys Glu Glu Ser Leu Leu Gin 835 840 845 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling out this page) ATG GCA GCT AAT CAG GTG GAG AAG GAT CTA ACA AAA CTG ACC AGT TGG 2592

Met Ala Ala Asn Gin Val Glu Lys Asp Leu Thr Lys Leu Thr Ser Trp 850 855 860 ACA CAG Απ GAC GCT ATT CTG CM TGG TTA CAG ATG TCT TOG GCC TTG 2640

Thr Gin lie Asp Ala He Leu Gin Trp Leu Gin Met Ser Ser Ala Leu 865 870 875 880 GCG GTT TCT CCA CTG GAT CTG GCA GGG ATG ATG GCC CTG AAA TAT GGG 2688 ------- m- This paper size applies China National Standard (CNS) A4 specification (210X297 mm) 509722 A7 B7 V. Description of invention (I92)

Ala Val Ser Pro Leu Asp Leu Ala Gly Met Met Ala Leu Lys Tyr Gly 885 890 895 ATA GAT CAT AAC TAT GCT GCC TGG CAA GCT GCG GCG GCT GCG CTG ATG 2736 lie Asp His Asn Tyr Ala Ala Trp Gin Ala Ala Ala Ala Ala Leu Met 900 905 910 GCT GAT CAT GCT AAT CAG GCA CAG AAA AAA CTG GAT GAG AOG TTC AGT 2784

Ala Asp His Ala Asn Gin Ala Gin Lys Lys Leu Asp Glu Thr Phe Ser 915 920 925 AAG GCA m TGT AAC TAT TAT Απ AAT GCT (ΠΤ GTC GAT AGT GCT GCT 2832

Lys Ala Leu Cys Asn Tyr Tyr lie Asn Ala Val Val Asp Ser Ala Ala 930 935 940 GGA GTA CX5T GAT CGT AAC GGT m TAT ACC TAT TIG CTG ΚΠ GAT AAT 2880

Gly Val Arg Asp Arg Asn Gly Leu Tyr Thr Tyr Leu Leu He Asp Asn 945 950 955 960 CAG GTT TCT GCC GAT GTG ATC ACT TCA CGT Απ GCA GAA GCT ATC GCC 2928

Gin Val Ser Ala Asp Val He Thr Ser Arg He Ala Glu Ala lie Ala 965 970 975 GGT Απ CAA CTG TAC GTT AAC OGG GCTTTA AAC CGA GAT GAA GGT CAG 2976

Gly lie Gin Leu Tyr Val Asn Arg Ala Leu Asn Arg Asp Glu Gly Gin 980 985 990 CTT GCA TCG GAC GTT AGT ACC CGT CAG TTC TTC ACT GAC TGG GAA CGT 3024

Leu Ala Ser Asp Val Ser Thr Arg Gin Phe Phe Thr Asp Trp Glu Arg 995 1000 1005 TAC MT AAA CGT TAC AGT ACT TGG GCT GGT GTC TCT GM CTG GTC TAT 3072 (Notes on the back then fill out this page)

Tyr Asn Lys Arg Tyr Ser Thr Trp Ala Gly Val Ser Glu Leu Val Tyr 1010 1015 1020 TAT CCA GAA AAC TAT GTT GAT CCC ACT CAG CGC Απ GGG CAA Α (Γ AAA 3120

Tyr Pro Glu Asn Tyr Val Asp Pro Thr Gin Arg He Gly Gin Thr Lys 1025 1030 1035 1040 ATG ATG GAT GCG CTG TTG CAA TCC ATC AAC CAG AGC CAG CTA AAT C3CG 3168

Met Met Asp Ala Leu Leu Gin Ser He Asn Gin Ser Gin Leu Asn Ala 1045 1050 1055 __- 195- This paper size applies to China National Standard (CNS) A4 specifications (210X 297 mm) 509722 A7 B7 V. Description of the invention (193 ) GAT ACXj GTG GAA GAT GCT TTC AAA ACT TAT TTG ACC AGC ΉΤ GAG CAG 3216

Asp Thr Val Glu Asp Ala Phe Lys Thr Tyr Leu Thr Ser Phe Glu Gin 1060 1065 1070 GTA GCA AAT CTG AAA GTA Απ AGT GCT TAC CAC GAT AAT GTG AAT GTG 3264

Val Ala Asn Leu Lys Val lie Ser Ala Tyr His Asp Asn Val Asn Val 1075 1080 1085 GAT CM GGA ΤΓΑ ACT TAT ΉΤ ATC GGT ATC GAC CM GCA GCT (XG GGT 3312

Asp Gin Gly Leu Thr Tyr Phe lie Gly lie Asp Gin Ala Ala Pro Gly 1090 1095 1100 ACG TAT TAC TGG CGT AGT C5TT GAT CAC AGC AAA TGT GAA AAT GGC AAG 3360

Thr Tyr Tyr Trp Arg Ser Val Asp His Ser Lys Cys Glu Asn Gly Lys 1105 1110 1115 1120 ΉΤ GCC GCT MT GCT TGG GGT GAG TGG MT AAA ATT ACC TGT GCT GTC 3408

Phe Ala Ala Asn Ala Trp Gly Glu Trp Asn Lys He Thr Cys Ala Val 1125 1130 1135 MT CCT TGG AAA MT ATC ATC CGT CCG GTT GTT TAT ATC TCC CGC m 3456

Asn Pro Trp Lys Asn He He Arg Pro Val Val Tyr Met Ser Arg Leu 1140 1145 1150 TAT CTO CTA TGG CTG GAG CAG CM TCA AAG AAA AGT GAT GAT GGT AAA 3504

Tyr Leu Leu Trp Leu Glu Gin Gin Ser Lys Lys Ser Asp Asp Gly Lys 1155 1160 1165 ACC ACG ATT TAT CAA TAT AAC m AAA CTG GCT CAT ATT CGT TAC GAC 3552

Thr Thr He Tyr Gin Tyr Asn Leu Lys Leu Ala His He Arg Tyr Asp 1170 1175 1180 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (please read the notes on the back before filling this page) GGT AGT TGG AAT ACA CTA TIT ACTTIT GAT GTG ACA GAA AAG GTA AAA 3600

Gly Ser Trp Asn Thr Pro Phe Thr Phe Asp Val Thr Glu Lys Val Lys 1185 1190 1195 1200 MT TAC ACG TOG AGT ACT GAT GCT GCT GAA TCT TTA GGG TTG TAT TGT 3648

Asn Tyr Thr Ser Ser Thr Asp Ala Ala Glu Ser Leu Gly Leu Tyr Cys 1205 1210 1215 ACT GGT TAT CAA GC5G GAA GAC ACT CTA m GTT ATG TTC TAT TCG ATG 3696 This paper is in accordance with the Chinese National Standard (CNS) A4 specification (210X297 (Mm) 509722 A7 B7 V. Description of the invention (194)

Thr Gly Tyr Gin Gly Glu Asp Thr Leu Leu Val Met Phe Tyr Ser Met 1220 1225 1230 CAG AGT ACT TAT AGC TCC TAT ACC GAT AAT MT GOG CCG GTC ACT GGG 3744

Gin Ser Ser Tyr Ser Ser Tyr Thr Asp Asn Asn Ala Pro Val Thr Gly 1235 1240 1245 CTA TAT ATT TTC GCT GAT ATG TCA TCA GAC MT ATG ACG AAT GCA CM 3792

Leu Tyr lie Phe Ala Asp Met Ser Ser Asp Asn Met Thr Asn Ala Gin 1250 1255 1260 GCA ACT AAC TAT TGG AAT AAC AGT TAT CCG CM TIT GAT ACT GTG ATG 3840

Ala Thr Asn Tyr Trp Asn Asn Ser Tyr Pro Gin Phe Asp Thr Val Met 1265 1270 1275 1280 GCA GAT CCG GAT AGC GAC AAT AAA AAA GTC ATA ACC AGA AGA GIT AAT 3888

Ala Asp Pro Asp Ser Asp Asn Lys Lys Val lie Thr Arg Arg Val Asn 1285 1290 1295 AAC CGT TAT GCG GAG GAT TAT GM ATT CCT TCC TCT GTG ACA AGT MC 3936

Asn Arg Tyr Ala Glu Asp Tyr Glu lie Pro Ser Ser Val Thr Ser Asn 1300 1305 1310 AGT AAT TAT TCT TCT TGG GGT GAT CAC AGT ΤΓΑ ACC ATG CTT TAT GGT C3GT 3984

Ser Asn Tyr Ser Trp Gly Asp His Ser Leu Thr Met Leu Tyr Gly Gly 1315 1320 1325 AGT GTT CCT AAT ΚΉ ACT ΤΓΤ GAA TCG (ΠΪ GCA GAA GAT m AGG CTA 4032

Ser Val Pro Asn He Thr Phe Glu Ser Ala Ala Glu Asp Leu Arg Leu 1330 1335 1340 TCT ACC AAT ATG GCA TTG AGT Απ ATT CAT AAT GGA TAT GCG GGA ACT 4080 (Notes on the back then fill out this page)

Ser Thr Asn Met Ala Leu Ser He He Asn Gly Tyr Ala Gly Thr 1345 1350 1355 1360 CGC CGT ATA CAA TGT AAT CTT ATG AAA CM TAC GCT TCA m GGT GAT 4128

Arg Arg He Gin Cys Asn Leu Met Lys Gin Tyr Ala Ser Leu Gly Asp 1365 1370 1375 AAA TTT ATA ATT TAT GAT TCA TCA ΤΓΤ GAT GAT GCA AAC CGT TTT AAT 4176

Lys Phe lie He Tyr Asp Ser Ser Phe Asp Asp Ala Asn Arg Phe Asn 1380 1385 1390 -m—— This paper size applies to Chinese National Standard (CNS) A4 specification (210X297 mm) 509722 A7 B7 V. Description of the invention (I95 ) CTG GTG CCA TTG TTT AAA TTC GGA AAA GAC GAG AAC TCA GAT GAT AGT 4224

Leu Val Pro Leu Phe Lys Phe Gly Lys Asp Glu Asn Ser Asp Asp Ser 1395 1400 1405 Απ TGT ATA TAT AAT GAA AAC CCT TCX: TCT GAA GAT AAG AAG TGG TAT 4272

He Cys lie Tyr Asn Glu Asn Pro Ser Ser Glu Asp Lys Lys Trp Tyr 1410 1415 1420 ΉΤ TCT TCG AAA GAT GAC AAT AAA ACA GOG GAT TAT AAT GGT GGA ACT 4320

Phe Ser Ser Lys Asp Asp Asn Lys Thr Ala Asp Tyr Asn Gly Gly Thr 1425 1430 1435 1440 CM TGT ATA GAT GCT GGA ACX: AGT AAC AAA GAT TIT TAT TAT AAT CTC 4368

Gin Cys lie Asp Ala Gly Thr Ser Asn Lys Asp Phe Tyr Tyr Asn Leu 1445 1450 1455 CAG GAG ATT GAA GTA Απ ACT C5TT ACT GGT GGG TAT TGG TCG AGT TAT 4416

Gin Glu He Glu Val lie Ser Val Thr Gly Gly Tyr Trp Ser Ser Tyr 1460 1465 1470 AAA ATA TCC AAC CCG Απ AAT ATC AAT ACXj GGC Απ GAT AGT GCT AAA 4464

Lys He Ser Asn Pro He Asn He Asn Thr Gly lie Asp Ser Ala Lys 1475 1480 1485 GTA AAA GTC ACC GTA AAA GCG GGT GGT GAC GAT CM ATC ΉΤ ACT GCT 4512

Val Lys Val Thr Val Lys Ala Gly Gly Asp Asp Gin lie Phe Thr Ala 1490 1495 1500 GAT AAT AGT ACC TAT GTT CCT CAG CM COG GCA CCC AGT ΉΤ GAG GAG 4560

Asp Asn Ser Thr Tyr Val Pro Gin Gin Pro Ala Pro Ser Phe Glu Glu 1505 1510 1515 1520 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling out this page) ATG Απ TAT CAG TTC AAT AAC CTG ACA ATA GAT TGT AAG AAT m AAT 4608

Met He Tyr Gin Phe Asn Asn Leu Thr lie Asp Cys Lys Asn Leu Asn 1525 1530 1535 TTC ATC GAC AAT CAG GCA CAT Απ GAG Απ GAT TTC ACC GCT ACG GCA 4656

Phe lie Asp Asn Gin Ala His lie Glu lie Asp Phe Thr Ala Thr Ala 1540 1545 1550 CM GAT GGC CGA TTC TTG GGT GCA GAA ACT TTT ATT ATC CCG GTA ACT 4704 ______,. This paper standard applies to China National Standard (CNS) A4 Specifications (210X 297 mm) 509722 A7 B7 V. Description of the invention (196)

Gin Asp Gly Arg Phe Leu Gly Ala Glu Thr Phe He lie Pro Val Thr 1555 1560 1565 AAA AAA GIT CTC GGT ACT GAG AAC GTG ATT GCG m TAT AGC GAA AAT 4752

Lys Lys Val Leu Gly Thr Glu Asn Val lie Ala Leu Tyr Ser Glu Asn (Please read the notes on the back before filling this page) 1570 1575 1580 AAC GGT GTT CM TAT ATG CM Απ GGC GCA TAT CGT ACC CXjTTTG AAT 4800

Asn Gly Val Gin Tyr Met Gin lie Gly Ala Tyr Arg Thr Arg Leu Asn 1585 1590 1595 1600 ACG ΤΓΑ TTC GCT CM CAG TTC GTT AGC OGT GCT MT OGT GGC ATT GAT 4848

Thr Leu Phe Ala Gin Gin Leu Val Ser Arg Ala Asn Arg Gly lie Asp 1605 1610 1615 GCA GTG CTC AGT ATG GAA ACT CAG AAT ΚΠ CAG GAA CGG CM m GGA 4896

Ala Val Leu Ser Met Glu Thr Gin Asn lie Gin Glu Pro Gin Leu Gly 1620 1625 1630 GCG C5GC ACA TAT GTC CAG CTT GTG TKj GAT AAA TAT GAT GAG TCT Απ 4944

Ala Gly Thr Tyr Tyr Val Gin Leu Val Leu Asp Lys Tyr Asp Glu Ser He 1635 1640 1645 CAT (XK: ACT AAT AAA AGC TIT GCT Απ GAA TAT GTT GAT ATA TTT AAA 4992

His Gly Thr Asn Lys Ser Phe Ala lie Glu Tyr Val Asp He Phe Lys 1650 1655 1660 GAG AAC GAT AGT TTT GTG Απ TAT CM GGA GAA CTT AC3C GAA ACA AGT 5040

Glu Asn Asp Ser Phe Val He Tyr Gin Gly Glu Leu Ser Glu Thr Ser 1665 1670 1675 1680 CM ACT GTT GTG AM GTT TTC m TO: TAT TIT ATA GAG GCXj ACT GGA 5088

Gin Thr Val Val Lys Val Phe Leu Ser Tyr Phe lie Glu Ala Thr Gly 1685 1690 1695 AAT AAG AAC CAC m TCG GTA CGT GCT AAA TAC CAA AAG GAA kCG ACT 5136

Asn Lys Asn His Leu Trp Val Arg Ala Lys Tyr Gin Lys Glu Thr Thr 1700 1705 1710 GAT AAG ATC TTG TTC GAC (UT ACT GAT GAG AAA GAT CCG CAC GGT TGG 5184

Asp Lys He Leu Phe Asp Arg Thr Asp Glu Lys Asp Pro His Gly Trp 1715 1720 1725 This paper size applies to Chinese National Standard (CNS) A4 specification (21〇X29l & cent) 509722 A7 B7 V. Description of invention (197) TIT CTC AGC GAC GAT CAC AAG ACC m AGT GGT CTC TCT TCC GCA CAG 5232

Phe Leu Ser Asp Asp His Lys Thr Phe Ser Gly Leu Ser Ser Ala Gin 1730 1735 1740 GCA TTA AAG AAC GAC AGT GAA CCG ATC GAT TTC TCT G (K: GCC AAT GCT 5280

Ala Leu Lys Asn Asp Ser Glu Pro Met Asp Phe Ser Gly Ala Asn Ala 1745 1750 1755 1760 CTC TAT TTC TGG GAA CTG TO TAT TAC ACG CCXj ΑΊΌ ATG ATG GCT CAT 5328

Leu Tyr Phe Trp Glu Leu Phe Tyr Tyr Thr Pro Met Met Met Ala His 1765 1770 1775 CXjUTG πθ CAG GAA CAG AAT TIT GAT GCG GCXj AAC CAT TGG TO CGT 5376

Arg Leu Leu Gin Glu Gin Asn Phe Asp Ala Ala Asn His Trp Phe Arg 1780 1785 1790 TAT GTC TGG AGT CCA TGC GGT TAT ATC GTT GAT GGT AAA Απ GCT ATC 5424

Tyr Val Trp Ser Pro Ser Gly Tyr lie Val Asp Gly Lys lie Ala lie 1795 1800 1805 TAC CAC TO MC GTG CGA CCG CTG GAA GAA GAC ACT AGT TGG AAT GCA 5472

Tyr His Trp Asn Val Arg Pro Leu Glu Glu Asp Thr Ser Trp Asn Ala 1810 1815 1820 CM CM CTG GAC TCC ACC GAT CCA GAT GCT GTA GCC CM GAT GAT CCG 5520

Gin Gin Leu Asp Ser Thr Asp Pro Asp Ala Val Ala Gin Asp Asp Pro 1825 1830 1835 1840 ATG CAC TAC AAG GTG GCT ACC TTT ATG GCG ACG TTG GAT CTG CTA ATG 5568

Met His Tyr Lys Val Ala Thr Phe Met Ala Thr Leu Asp Leu Leu Met 1845 1850 1855 Printed by the Staff Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (please read the notes on the back before filling this page) GCC CGT GGT GAT GCT GCT TAC CGC CAG m GAG CGT GAT ACG TTG GCT 5616

Ala Arg Gly Asp Ala Ala Tyr Arg Gin Leu Glu Arg Asp Thr Leu Ala 1860 1865 1870 GM GCT AAA ATG TGG TAT ACA CAG GCG CTT AAT CTG TTG GGT GAT GAG 5664

Glu Ala Lys Met Trp Tyr Thr Gin Ala Leu Asn Leu Leu Gly Asp Glu 1875 1880 1885 CCA CAA GTG ATG CTG AGT ACXj ACT TGG GCT AAT CCA ACA TTG GGT AAT 5712 -200- This paper standard applies to Chinese National Standard (CNS) A4 Specifications (210X 297 mm) 509722 A7 __B7_ V. Description of the invention (l98)

Pro Gin Val Met Leu Ser Thr Thr Trp Ala Asn Pro Thr Leu Gly Asn 1890 1895 1900 GCT GCT TCA AAA ACC ACA CAG CAG (ΠΤ CGT CAG CM GTG CTT ACC CAG 5760

Ala Ala Ser Lys Thr Thr Gin Gin Val Arg Gin Gin Val Leu Thr Gin 1905 1910 1915 1920 TTG CGT CTC MT AGC AGG GTA AAA ACC CCG TTG CTA GGA ACA GCC MT 5808

Leu Arg Leu Asn Ser Arg Val Lys Thr Pro Leu Leu Gly Thr Ala Asn 1925 1930 1935 TCC ΟΌ ACC GCT m Ή ×: CTG CCG CAG GAA MT AGC AAG CTC AAA GGC 5856

Ser Leu Thr Ala Leu Phe Leu Pro Gin Glu Asn Ser Lys Leu Lys Gly 1940 1945 1950 TAC TGG CGG ACA CTG GCG CAG CGT ATG ΉΤ MT m CGT CAT MT CTG 5904

Tyr Trp Arg Thr Leu Ala Gin Arg Met Phe Asn Leu Arg His Asn Leu 1955 1960 1965 TCG ATT GAC GGC CAG CCG CTC TOC TTG CCG CTG TAT GCT AAA CCG GCT 5952

Ser He Asp Gly Gin Pro Leu Ser Leu Pro Leu Tyr Ala Lys Pro Ala 1970 1975 1980 GAT CCA AM GCT m CTC ACT GCG (Π; GTT TCA GCT TCT CAA GGG GGA 6000

Asp Pro Lys Ala Leu Leu Ser Ala Ala Val Ser Ala Ser Gin Gly Gly 1985 1990 1995 2000 GCC GAC TTG CCG MG GCG CCG CTG ACT ATT CAC CGC TTC CCT CM ATG 6048

Ala Asp Leu Pro Lys Ala Pro Leu Thr He His Arg Phe Pro Gin Met 2005 2010 2015 CTA GM GGG GCA CGG GGC TTG GTT MC CAG CTT ATA CAG TTC GGT AGT 6096 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (Please read first (Notes on the back then fill out this page)

Leu Glu Gly Ala Arg Gly Leu Val Asn Gin Leu He Gin Phe Gly Ser 2020 2025 2030 TCA CTA TTG GGG TAC AGT GAG CGT CAG GAT GCG GM GCT ATG AGT CAA 6144

Ser Leu Leu Gly Tyr Ser Glu Arg Gin Asp Ala Glu Ala Met Ser Gin 2035 2040 2045 CTA CTG CAA ACC CAA GCC AGC GAG m ATA CTG ACC AGT Απ CGT ATG 6192

Leu Leu Gin Thr Gin Ala Ser Glu Leu He Leu Thr Ser He Arg Met 2050 2055 2060 -7Ω1-This paper size applies to China National Standard (CNS) A4 (210X 297 mm) 509722 A7 B7 V. Description of the invention (199 ) CAG GAT AAC CM TTG GCA GAG CTC GAT ΤΧχΪ GM AM ACC GO: TTG CAA 6240

Gin Asp Asn Gin Leu Ala Glu Leu Asp Ser Glu Lys Thr Ala Leu Gin 2065 2070 2075 2080 GTC TCT m GCT ΟΪΑ (ΠΌ CM CM CGG ITT GAC AGC TAT AGC CAA CTG 6288

Val Ser Leu Ala Gly Val Gin Gin Arg Phe Asp Ser Tyr Ser Gin Leu 2085 2090 2095 TAT GAG GAG AAC ATC AAC GCA GGT GAG CAG OJA GCXj CTO GCG ΠΑ CGC 6336

Tyr Glu Glu Asn He Asn Ala Gly Glu Gin Arg Ala Leu Ala Leu Arg 2100 2105 2110 TCA GAA TCT GCT Απ GAG TCT CAG GGA GCG CAG Απ 1TCC CGT ATG GCA 6384

Ser Glu Ser Ala lie Glu Ser Gin Gly Ala Gin lie Ser Arg Met Ala 2115 2120 2125 GGC GCG GGT GTT GAT ATC GCA CCA MT ATC TTC GGC CTG GCT GAT GGC 6432

Gly Ala Gly Val Asp Met Ala Pro Asn He Phe Gly Leu Ala Asp Gly 2130 2135 2140 GGC ATG CAT TAT GGT GCT Απ GCC TAT GCC ATC GCT GAC GGT Απ GAG 6480

Gly Met His Tyr Gly Ala He Ala Tyr Ala lie Ala Asp Gly lie Glu 2145 2150 2155 2160 ΉΌ AGT GCT TCT GCC AAG ATG GTT GAT C3CG GAG AAA GTT GCT CAG TOG 6528

Leu Ser Ala Ser Ala Lys Met Val Asp Ala Glu Lys Val Ala Gin Ser 2165 2170 2175 GM ATA TAT CGC CGT CGC CGT CM GAA TGG AAA ATT CAG CGT GAC AAC 6576

Glu He Tyr Arg Arg Arg Arg Gin Glu Trp Lys He Gin Arg Asp Asn 2180 2185 2190 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (please read the notes on the back before filling this page) GCA CM GCG GAG Απ AAC CAG HA MC GCG CAA CTG GM TCA CTG TCT 6624

Ala Gin Ala Glu He Asn Gin Leu Asn Ala Gin Leu Glu Ser Leu Ser 2195 2200 2205 Απ OjC CGT GAA GCC GCT GAA ATG CAA AAA GAG TAC CTG AAA ACC CAG 6672

He Arg Arg Glu Ala Ala Glu Met Gin Lys Glu Tyr Leu Lys Thr Gin 2210 2215 2220 CAA GCT CAG GCG CAG GCA CAA CTT ACTTTC m AGA AGC AAA TTC AGT 6720 -m- This paper size applies to Chinese National Standard (CNS) A4 specifications (210X297 mm) 509722 A7 B7 V. Description of the invention (200)

Gin Ala Gin Ala Gin Ala Gin Leu Thr Phe Leu Arg Ser Lys Phe Ser 2225 2230 2235 2240 MT CM GCG m TAT AGT TGG m CGA GGG CGT TTG TCA GGT ATT TAT 6768

Asn Gin Ala Leu Tyr Ser Trp Leu Arg Gly Arg Leu Ser Gly lie Tyr 2245 2250 2255 TTC CAG TTC TAT GAC TTC GCC GTA TCA CGT TXK: CTXJ ATG GCA GAG CAA 6816

Phe Gin Phe Tyr Asp Leu Ala Val Ser Arg Cys Leu Met Ala Glu Gin 2260 2265 2270 TCC TAT CAA TGG GAA GCT AAT GAT MT TO: Απ A (K: TIT GTC AAA COG 6864

Ser Tyr Gin Trp Glu Ala Asn Asp Asn Ser lie Ser Phe Val Lys Pro 2275 2280 2285 GGT GCA TGG CM GGA ACT TAC GCC GGC ΤΓΑ TTG TGT GGA GM GCT TTG 6912

Gly Ala Trp Gin Gly Thr Tyr Ala Gly Leu Leu Cys Gly Glu Ala Leu 2290 2295 2300 ATA CAA AAT CTG GCA CAA ATG GAA GAG GCA TAT CTG AAA TGG GAA TCT 6960 lie Gin Asn Leu Ala Gin Met Glu Glu Ala Tyr Leu Ly Glu Ser 2305 2310 2315 2320 CGC GCTTTG GAA (3TA GAA CGC ACG GTT TCA TTG GCA GTG GTT TAT GAT 7008

Arg Ala Leu Glu Val Glu Arg Thr Val Ser Leu Ala Val Val Tyr Asp 2325 2330 2335 TCA CTG GAA GGT AAT GAT CGT ΤΤ AAT m GCG GAA CAA ATA CCT GCA 7056

Ser Leu Glu Gly Asn Asp Arg Phe Asn Leu Ala Glu Gin lie Pro Ala 2340 2345 2350 m TTG GAT MG GMG GAG GGA ACA GCA GGA ACT AAA GAA AAT GGG m 7104 (Notes on the back then fill out this page)

Leu Leu Asp Lys Gly Glu Gly Thr Ala Gly Thr Lys Glu Asn Gly Leu 2355 2360 2365 TCA TTG GCT MT GCT ATC CTG TCA GCT TOG GTC AAA TTG TCC GAC TTG 7152

Ser Leu Ala Asn Ala He Leu Ser Ala Ser Val Lys Leu Ser Asp Leu 2370 2375 2380 AAA CTG GGA ACG GAT TAT CCA GAC AGT ATC GTT GGT AGC AAC AAG GIT 7200

Lys Leu Gly Thr Asp Tyr Pro Asp Ser He Val Gly Ser Asn Lys Val 2385 2390 2395 2400 __3¾- This paper is again applicable to China National Standard (CNS) A4 (210 X 297 mm) 509722 Employees of the Central Standards Bureau of the Ministry of Economic Affairs Printed by the cooperative A7 B7 V. Description of the invention (201) CGT CGT Απ MG CM ATC AGT GTT TCG CTA CCT GCA TTG GTT GGG CCT 7248

Arg Arg lie Lys Gin lie Ser Val Ser Leu Pro Ala Leu Val Gly Pro 2405 2410 2415 TAT CAG GAT GTT CAG GCT ATG CTC AGC TAT GOT GO: AGT ACT CAA TTG 7296

Tyr Gin Asp Val Gin Ala Met Leu Ser Tyr Gly Gly Ser Thr Gin Leu 2420 2425 2430 CCG AM GGT TGT TCA GCG TTG GCT GTG TCT CAT GGT ACC MT GAT AGT 7344

Pro Lys Gly Cys Ser Ala Leu Ala Val Ser His Gly Thr Asn Asp Ser 2435 2440 2445 GGT CAG TTC CAG TTG GAT TTC AAT GAC G (X: AM TAC 〇ΧΪ CCA ΤΓΤ GAA 7392

Gly Gin Phe Gin Leu Asp Phe Asn Asp Gly Lys Tyr Leu Pro Phe Glu 2450 2455 2460 GGT Απ GCT CTT GAT GAT CAG GGT ACA CTG AAT CTT CM Ή CC CCG MT 7440

Gly He Ala Leu Asp Asp Gin Gly Thr Leu Asn Leu Gin Phe Pro Asn 2465 2470 2475 2480 GCT ACC GAC AAG CAG AAA GCA ATA TTG CM ACT ATG AGC GAT Απ Απ 7488

Ala Thr Asp Lys Gin Lys Ala lie Leu Gin Thr Met Ser Asp He He 2485 2490 2495 TTG CAT ATT CGT TAT ACC ATC CGT TAA 7515

Leu His lie Arg Tyr Thr He Arg * 2500 2505 资料 Data of sequence identification number 12: (i) Sequence characteristics: (A) Length: 2504 amino acids (B) Type: amino acid (D) Linear (ii) Molecular type: Protein (xi) Sequence description: Sequence identification number 12 (TcbA protein):

Met Gin Asn Ser Leu Ser Ser Thr lie Asp Thr He Cys Gin Lys Leu 15 10 15

Gin Leu Thr Cys Pro Ala Glu lie Ala Leu Tyr Pro Phe Asp Thr Phe 20 25 30 This paper size applies to China National Standard (CNS) A4 size (210X 297 mm) (Please read the precautions on the back before filling this page) Order 4 509722 Employees' Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs, India and India A7 B7 V. Description of Invention

Arg Glu Lys Thr Arg Gly Met Val Asn Trp Gly Glu Ala Lys Arg lie 35 40 45

Tyr Glu He Ala Gin Ala Glu Gin Asp Arg Asn Leu Leu His Glu Lys 50 55 60

Arg lie Phe Ala Tyr Ala Asn Pro Leu Leu Lys Asn Ala Val Arg Leu 65 70 75 80

Gly Thr Arg Gin Met Leu Gly Phe He Gin Gly Tyr Ser Asp Leu Phe 85 90 95

Gly Asn Arg Ala Asp Asn Tyr Ala Ala Pro Gly Ser Val Ala Ser Met 100 105 110

Phe Ser Pro Ala Ala Tyr Leu Thr Glu Leu Tyr Arg Glu Ala Lys Asn 115 120 125

Leu His Asp Ser Ser Ser lie Tyr Tyr Leu Asp Lys Arg Arg Pro Asp 130 135 140

Leu Ala Ser Leu Met Leu Ser Gin Lys Asn Met Asp Glu Glu lie Ser 145 150 155 160

Thr Leu Ala Leu Ser Asn Glu Leu Cys Leu Ala Gly lie Glu Thr Lys 165 170 175

Thr Gly Lys Ser Gin Asp Glu Val Met Asp Met Leu Ser Thr Tyr Arg 180 185 190

Leu Ser Gly Glu Thr Pro Tyr His His Ala Tyr Glu Thr Val Arg Glu 195 200 205

He Val His Glu Arg Asp Pro Gly Phe Arg His Leu Ser Gin Ala Pro 210 215 220

He Val Ala Ala Lys Leu Asp Pro Val Thr Leu Leu Gly He Ser Ser 225 230 235 240

His He Ser Pro Glu Leu Tyr Asn Leu Leu lie Glu Glu He Pro Glu 245 250 255 ------ 7〇 ^-This paper size applies to China National Standard (CNS) A4 specifications (210, X29 < 7 mm) (Please read the notes on the back before filling this page)

509722 A7 B7 V. Description of the invention (203)

Lys Asp Glu Ala Ala Leu Asp Thr Leu Tyr Lys Thr Asn Phe Gly Asp 260 265 270 lie Thr Thr Ala Gin Leu Met Ser Pro Ser Tyr Leu Ala Arg Tyr Tyr 275 280 285

Gly Val Ser Pro Glu Asp lie Ala Tyr Val Thr Thr Ser Leu Ser His 290 295 300

Val Gly Tyr Ser Ser Asp He Leu Val He Pro Leu Val Asp Gly Val 305 310 315 320

Gly Lys Met Glu Val Val Arg Val Thr Arg Thr Pro Ser Asp Asn Tyr 325 330 335

Thr Ser Gin Thr Asn Tyr lie Glu Leu Tyr Pro Gin Gly Gly Asp Asn 340 345 350

Tyr Leu lie Lys Tyr Asn Leu Ser Asn Ser Phe Gly Leu Asp Asp Phe 355 360 365

Tyr Leu Gin Tyr Lys Asp Gly Ser Ala Asp Trp Thr Glu He Ala His 370 375 380

Asn Pro Tyr Pro Asp Met Val lie Asn Gin Lys Tyr Glu Ser Gin Ala 385 390 395 400

Thr He Lys Arg Ser Asp Ser Asp Asn He Leu Ser lie Gly Leu Gin 405 410 415 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling out this page)

Arg Trp His Ser Gly Ser Tyr Asn Phe Ala Ala Ala Asn Phe Lys He 420 425 430

Asp Gin Tyr Ser Pro Lys Ala Phe Leu Leu Lys Met Asn Lys Ala He 435 440 445

Arg Leu Leu Lys Ala Thr Gly Leu Ser Phe Ala Thr Leu Glu Arg He 450 455 460

Val Asp Ser Val Asn Ser Thr Lys Ser He Thr Val Glu Val Leu Asn 465 470 475 480 -------- 206- This paper size applies to China National Standard (CNS) A4 (210X: 297 mm) 509722 A7 B7 V. Description of the invention (204)

Lys Val Tyr Arg Val Lys Phe Tyr He Asp Arg Tyr Gly He Ser Glu 485 490 495

Glu Thr Ala Ala lie Leu Ala Asn lie Asn lie Ser Gin Gin Ala Val 500 505 510

Gly Asn Gin Leu Ser Gin Phe Glu Gin Leu Phe Asn His Pro Pro Leu 515 520 525

Asn Gly lie Arg Tyr Glu He Ser Glu Asp Asn Ser Lys His Leu Pro 530 535 540

Asn Pro Asp Leu Asn Leu Lys Pro Asp Ser Thr Gly Asp Asp Gin Arg 545 550 555 560

Lys Ala Val Leu Lys Arg Ala Phe Gin Val Asn Ala Ser Glu Leu Tyr 565 570 575

Gin Met Leu Leu lie Thr Asp Arg Lys Glu Asp Gly Val He Lys Asn 580 585 590

Asn Leu Glu Asn Leu Ser Asp Leu Tyr Leu Val Ser Leu Leu Ala Gin 595 600 605 lie His Asn Leu Thr He Ala Glu Leu Asn He Leu Leu Val lie Cys 610 615 620

Gly Tyr Gly Asp Thr Asn He Tyr Gin He Thr Asp Asp Asn Leu Ala 625 630 635 640

Lys lie Val Glu Thr Leu Leu Trp He Thr Gin Trp Leu Lys Thr Gin Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling out this page) 645 650 655

Lys Trp Thr Val Thr Asp Leu Phe Leu Met Thr Thr Ala Thr Tyr Ser 660 665 670

Thr Thr Leu Thr Pro Glu lie Ser Asn Leu Thr Ala Thr Leu Ser Ser 675 680 685

Thr Leu His Gly Lys Glu Ser Leu He Gly Glu Asp Leu Lys Arg Ala 690 695 700 __— This paper size applies to China National Standard (CNS) A4 (210X 297 mm) 509722 A7 B7 V. Description of the invention (205 )

Met Ala Pro Cys Phe Thr Ser Ala Leu His Leu Thr Ser Gin Glu Val 705 710 715 720

Ala Tyr Asp Leu Leu Leu Trp lie Asp Gin lie Gin Pro Ala Gin lie 725 730 735

Thr Val Asp Gly Phe Trp Glu Glu Val Gin Thr Thr Pro Thr Ser Leu 740 745 750

Lys Val He Thr Phe Ala Gin Val Leu Ala Gin Leu Ser Leu lie Tyr 755 760 765

Arg Arg He Gly Leu Ser Glu Thr Glu Leu Ser Leu He Val Thr Gin 770 775 780

Ser Ser Leu Leu Val Ala Gly Lys Ser He Leu Asp His Gly Leu Leu 785 790 795 800

Thr Leu Met Ala Leu Glu Gly Phe His Thr Trp Val Asn Gly Leu Gly 805 810 815

Gin His Ala Ser Leu lie Leu Ala Ala Leu Lys Asp Gly Ala Leu Thr 820 825 830

Val Thr Asp Val Ala Gin Ala Met Asn Lys Glu Glu Ser Leu Leu Gin 835 840 845

Met Ala Ala Asn Gin Val Glu Lys Asp Leu Thr Lys Leu Thr Ser Trp 850 855 860

Thr Gin He Asp Ala lie Leu Gin Trp Leu Gin Met Ser Ser Ala Leu Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling out this page) 865 870 875 880

Ala Val Ser Pro Leu Asp Leu Ala Gly Met Met Ala Leu Lys Tyr Gly 885 890 895

He Asp His Asn Tyr Ala Ala Trp Gin Ala Ala Ala Ala Ala Leu Met 900 905 910

Ala Asp His Ala Asn Gin Ala Gin Lys Lys Leu Asp Glu Thr Phe Ser 915 920 925 This paper size is applicable to the Chinese National Standard (CNS) A4 (210 X 297 mm) 509722 Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs A7 B7 V. Description of Invention (206)

Lys Ala Leu Cys Asn Tyr Tyr He Asn Ala Val Val Asp Ser Ala Ala 930 935 940

Gly Val Arg Asp Arg Asn Gly Leu Tyr Thr Tyr Leu Leu He Asp Asn 945 950 955 960

Gin Val Ser Ala Asp Val He Thr Ser Arg He Ala Glu Ala He Ala 965 970 975

Gly lie Gin Leu Tyr Val Asn Arg Ala Leu Asn Arg Asp Glu Gly Gin 980 985 990

Leu Ala Ser Asp Val Ser Thr Arg Gin Phe Phe Thr Asp Trp Glu Arg 995 1000 1005

Tyr Asn Lys Arg Tyr Ser Thr Trp Ala Gly Val Ser Glu Leu Val Tyr 1010 1015 1020

Tyr Pro Glu Asn Tyr Val Asp Pro Thr Gin Arg He Gly Gin Thr Lys 1025 1030 1035 1040

Met Met Asp Ala Leu Leu Gin Ser He Asn Gin Ser Gin Leu Asn Ala 1045 1050 1055

Asp Thr Val Glu Asp Ala Phe Lys Thr Tyr Leu Thr Ser Phe Glu Gin 1060 1065 1070

Val Ala Asn Leu Lys Val He Ser Ala Tyr His Asp Asn Val Asn Val 1075 1080 1085

Asp Gin Gly Leu Thr Tyr Phe He Gly He Asp Gin Ala Ala Pro Gly 1090 1095 1100

Thr Tyr Tyr Trp Arg Ser Val Asp His Ser Lys Cys Glu Asn Gly Lys 1105 1110 1115 1120

Phe Ala Ala Asn Ala Trp Gly Glu Trp Asn Lys lie Thr Cys Ala Val 1125 1130 1135

Asn Pro Trp Lys Asn He He Arg Pro Val Val Tyr Met Ser Arg Leu 1140 1145 1150 ______________________________ 5Θ9—— This paper size applies to China National Standard (CNS) A4 size (210x297 mm) (Please read the notes on the back before filling in (This page)-installed _

1T 509722 A7 B7 Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs 5. Description of invention (207) Tyr Leu Leu Trp Leu Glu Gin Gin Ser Lys Lys Ser Asp Asp Gly Lys 1155 1160 1165 Thr Thr lie Tyr Gin Tyr Asn Leu Lys Leu Ala His He Arg Tyr Asp 1170 1175 1180 Gly Ser Trp Asn Thr Pro Phe Thr Phe Asp Val Thr Glu Lys Val Lys 1185 1190 1195 1200 Asn Tyr Thr Ser Ser Thr Asp Ala Ala Glu Ser Leu Gly Leu Tyr Cys 1205 1210 1215 Thr Gly Tyr Gin Gly Glu Asp Thr Leu Leu Val Met Phe Tyr Ser Met 1220 1225 1230 Gin Ser Ser Tyr Ser Ser Tyr Thr Asp Asn Asn Ala Pro Val Thr Gly 1235 1240 1245 Leu Tyr He Phe Ala Asp Met Ser Ser Asp Asn Met Thr Asn Ala Gin 1250 1255 1260 Ala Thr Asn Tyr Trp Asn Asn Ser Tyr Pro Gin Phe Asp Thr Val Met 1265 1270 1275 1280 Ala Asp Pro Asp Ser Asp Asn Lys Lys Val He Thr Arg Arg Val Asn 1285 1290 1295 Asn Arg Tyr Ala Glu Asp Tyr Glu He Pro Ser Ser Val Thr Ser Asn 1300 1305 1310 Ser Asn Tyr Ser Trp Gly Asp His Ser Leu Thr Met Leu Tyr Gly Gly 1315 1320 1325 Ser Val Pro Asn He Thr Phe Glu Ser Ala Ala Glu Asp Leu Arg Leu 1330 1335 1340 Ser Thr Asn Met Ala Leu Ser He He His Asn Gly Tyr Ala Gly Thr 1345 1350 1355 1360 Arg Arg lie lie Gin Cys Asn Leu Met Lys Gin Tyr Ala Ser Leu Gly Asp 1365 1370 1375 (Please read the notes on the back before filling out this page)-Binding · Ordering d ---— This paper size applies to China National Standard (CNS) A4 specification (210X 297 mm) 509722 A7 B7 V. Description of invention (208 )

Lys Phe lie lie Tyr Asp Ser Ser Phe Asp Asp Ala Asn Arg Phe Asn 1380 1385 1390

Leu Val Pro Leu Phe Lys Phe Gly Lys Asp Glu Asn Ser Asp Asp Ser 1395 1400 1405

He Cys He Tyr Asn Glu Asn Pro Ser Ser Glu Asp Lys Lys Trp Tyr 1410 1415 1420

Phe Ser Ser Lys Asp Asp Asn Lys Thr Ala Asp Tyr Asn Gly Gly Thr 1425 1430 1435 1440

Gin Cys He Asp Ala Gly Thr Ser Asn Lys Asp Phe Tyr Tyr Asn Leu 1445 1450 1455

Gin Glu lie Glu Val He Ser Val Thr Gly Gly Tyr Trp Ser Ser Tyr 1460 1465 1470

Lys He Ser Asn Pro He Asn He Asn Thr Gly He Asp Ser Ala Lys 1475 1480 1485

Val Lys Val Thr Val Lys Ala Gly Gly Asp Asp Gin He Phe Thr Ala 1490 1495 1500

Asp Asn Ser Thr Tyr Val Pro Gin Gin Pro Ala Pro Ser Phe Glu Glu 1505 1510 1515 1520

Met lie Tyr Gin Phe Asn Asn Leu Thr lie Asp Cys Lys Asn Leu Asn 1525 1530 1535 Printed by the Staff Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling out this page)

Phe lie Asp Asn Gin Ala His He Glu He Asp Phe Thr Ala Thr Ala 1540 1545 1550

Gin Asp Gly Arg Phe Leu Gly Ala Glu Thr Phe He lie Pro Val Thr 1555 1560 1565

Lys Lys Val Leu Gly Thr Glu Asn Val He Ala Leu Tyr Ser Glu Asn 1570 1575 1580

Asn Gly Val Gin Tyr Met Gin He Gly Ala Tyr Arg Thr Arg Leu Asn 1585 1590 1595 1600 This paper size applies to China National Standard (CNS) A4 (21 × 297 mm) 509722 Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs A7 B7 V. Description of the invention (209)

Thr Leu Phe Ala Gin Gin Leu Val Ser Arg Ala Asn Arg Gly lie Asp 1605 1610 1615

Ala Val Leu Ser Met Glu Thr Gin Asn He Gin Glu Pro Gin Leu Gly 1620 1625 1630

Ala Gly Thr Tyr Val Gin Leu Val Leu Asp Lys Tyr Asp Glu Ser lie 1635 1640 1645

His Gly Thr Asn Lys Ser Phe Ala He Glu Tyr Val Asp He Phe Lys 1650 1655 1660

Glu Asn Asp Ser Phe Val He Tyr Gin Gly Glu Leu Ser Glu Thr Ser 1665 1670 1675 1680

Gin Thr Val Val Lys Val Phe Leu Ser Tyr Phe lie Glu Ala Thr Gly 1685 1690 1695

Asn Lys Asn His Leu Trp Val Arg Ala Lys Tyr Gin Lys Glu Thr Thr 1700 1705 1710

Asp Lys lie Leu Phe Asp Arg Thr Asp Glu Lys Asp Pro His Gly Trp 1715 1720 1725

Phe Leu Ser Asp Asp His Lys Thr Phe Ser Gly Leu Ser Ser Ala Gin 1730 1735 1740

Ala Leu Lys Asn Asp Ser Glu Pro Met Asp Phe Ser Gly Ala Asn Ala 1745 1750 1755 1760

Leu Tyr Phe Trp Glu Leu Phe Tyr Tyr Thr Pro Met Met Met Ala His 1765 1770 1775

Arg Leu Leu Gin Glu Gin Asn Phe Asp Ala Ala Asn His Trp Phe Arg 1780 1785 1790

Tyr Val Trp Ser Pro Ser Gly Tyr He Val Asp Gly Lys lie Ala He 1795 1800 1805

Tyr His Trp Asn Val Arg Pro Leu Glu Glu Asp Thr Ser Trp Asn Ala 1810 1815 1820 This paper size applies to China National Standard (CNS) A4 specification (210X297 mm) ------- • installation-(please first Read the notes on the back and fill in this page) Order 509722 A7 B7 Printed by the Consumers' Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs 5. Description of the invention (210) Gin Gin Leu Asp Ser Thr Asp Pro Asp Ala Val Ala Gin Asp Asp Pro 1825 1830 1835 1840 Met His Tyr Lys Val Ala Thr Phe Met Ala Thr Leu Asp Leu Leu Met 1845 1850 1855 Ala Arg Gly Asp Ala Ala Tyr Arg Gin Leu Glu Arg Asp Thr Leu Ala 1860 1865 1870 Glu Ala Lys Met Trp Tyr Thr Gin Ala Asn Leu Leu Gly Asp Glu 1875 1880 1885 Pro Gin Val Met Leu Ser Thr Thr Trp Ala Asn Pro Thr Leu Gly Asn 1890 1895 1900 Ala Ala Ser Lys Thr Thr Gin Gin Val Arg Gin Gin Val Leu Thr Gin 1905 1910 1915 1920 Leu Arg Leu Asn Ser Arg Val Lys Thr Pro Leu Leu Gly Thr Ala Asn 1925 1930 1935 Ser Leu Thr Ala Leu Phe Leu Pro Gin Glu Asn Ser Lys Leu Lys Gly 1940 1945 1950 Tyr Trp Arg Thr Leu Ala Gin Arg Met Phe Asn Leu Arg His Asn Leu 1955 1960 1965 Ser He Asp Gly Gin Pro Leu Ser Leu Pro Leu Tyr Ala Lys Pro Ala 1970 1975 1980 Asp Pro Lys Ala Leu Leu Ser Ala Ala Val Ser Ala Ser Gin Gly Gly 1985 1990 1995 2000 Ala Asp Leu Pro Lys Ala Pro Leu Thr He His Arg Phe Pro Gin Met 2005 2010 2015 Leu Glu Gly Ala Arg Gly Leu Val Asn Gin Leu lie Gin Phe Gly Ser 2020 2025 2030 Ser Leu Leu Gly Tyr Ser Glu Arg Gin Asp Ala Glu Ala Met Ser Gin 2035 2040 2045 (Please read the notes on the back before filling out this page) · Binding. Order d This paper size is applicable to China National Standard (CNS) A4 specification (210X297 mm) 509722 A7 B7 V. Description of the invention (211)

Leu Leu Gin Thr Gin Ala Ser Glu Leu He Leu Thr Ser He Arg Met 2050 2055 2060

Gin Asp Asn Gin Leu Ala Glu Leu Asp Ser Glu Lys Thr Ala Leu Gin 2065 2070 2075 2080

Val Ser Leu Ala Gly Val Gin Gin Arg Phe Asp Ser Tyr Ser Gin Leu 2085 2090 2095

Tyr Glu Glu Asn He Asn Ala Gly Glu Gin Arg Ala Leu Ala Leu Arg 2100 2105 2110

Ser Glu Ser Ala He Glu Ser Gin Gly Ala Gin He Ser Arg Met Ala 2115 2120 2125

Gly Ala Gly Val Asp Met Ala Pro Asn lie Phe Gly Leu Ala Asp Gly 2130 2135 2140

Gly Met His Tyr Gly Ala lie Ala Tyr Ala lie Ala Asp Gly lie Glu 2145 2150 2155 2160

Leu Ser Ala Ser Ala Lys Met Val Asp Ala Glu Lys Val Ala Gin Ser 2165 2170 2175

Glu lie Tyr Arg Arg Arg Arg Gin Glu Trp Lys He Gin Arg Asp Asn 2180 2185 2190

Ala Gin Ala Glu He Asn Gin Leu Asn Ala Gin Leu Glu Ser Leu Ser 2195 2200 2205 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (please read the notes on the back before filling this page) lie Arg Arg Glu Ala Ala Glu Met Gin Lys Glu Tyr Leu Lys Thr Gin 2210 2215 2220

Gin Ala Gin Ala Gin Ala Gin Leu Thr Phe Leu Arg Ser Lys Phe Ser 2225 2230 2235 2240

Asn Gin Ala Leu Tyr Ser Trp Leu Arg Gly Arg Leu Ser Gly lie Tyr 2245 2250 2255

Phe Gin Phe Tyr Asp Leu Ala Val Ser Arg Cys Leu Met Ala Glu Gin 2260 2265 2270 This paper size applies to China National Standard (CNS) A4 (210X 297 mm) 509722 A7 B7 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs V. Description of the Invention (212) Ser Tyr Gin Trp Glu Ala Asn Asp Asn Ser He Ser Phe Val Lys Pro 2275 2280 2285 Gly Ala Trp Gin Gly Thr Tyr Ala Gly Leu Leu Cys Gly Glu Ala Leu 2290 2295 2300 lie Gin Asn Leu Ala Gin Met Glu Glu Ala Tyr Leu Lys Trp Glu Ser 2305 2310 2315 2320 Arg Ala Leu Glu Val Glu Arg Thr Val Ser Leu Ala Val Val Tyr Asp 2325 2330 2335 Ser Leu Glu Gly Asn Asp Arg Phe Asn Leu Ala Glu Gin He Pro Ala 2340 2345 2350 Leu Leu Asp Lys Gly Glu Gly Thr Ala Gly Thr Lys Glu Asn Gly Leu 2355 2360 2365 Ser Leu Ala Asn Ala He Leu Ser Ala Ser Val Lys Leu Ser Asp Leu 2370 2375 2380 Lys Leu Gly Thr Asp Tyr Pro Asp Ser lie Val Gly Ser Asn Lys Val 2385 2390 2395 2400 Arg Arg lie Lys Gin He Ser Val Ser Leu Pro Ala Leu Val Gly Pro 2405 2410 2415 Tyr Gin Asp Val Gin Ala Met Leu Ser Tyr Gly Gly Ser Thr Gin Leu 2420 2425 2430 Pro Lys Gly Cys Ser Ala Leu Ala Val Ser His Gly Thr Asn Asp Ser 2435 2440 2445 Gly Gin Phe Gin Leu Asp Phe Asn Asp Gly Lys Tyr Leu Pro Phe Glu 2450 2455 2460 Gly He Ala Leu Asp Asp Gin Gly Thr Leu Asn Leu Gin Phe Pro Asn 2465 2470 2475 2480 Ala Thr Asp Lys Gin Lys Ala He Leu Gin Thr Met Ser Asp lie lie 2485 2490 2495 (Please read the notes on the back before filling out this page ) 1 pack_, ^ τ d ___- 91S- This paper size applies to Chinese National Standard (CNS) A4 (210X 297 mm) 509722 A7 B7 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 5. Description of the invention (213) Leu His lie Arg Tyr Thr He Arg * 2500 2505 资料 Sequence ID No. 13: (i) Sequence characteristics: (A) Length: 12 amino acids (B) type · amino acids (C) strand Number: Single strand (D) Form: Linear (ii) Molecular type: Peptide (xi) Sequence description: Sequence identification number 13 (TcdAii N-terminus): Leu He Gly Tyr Asn Asin Gin Phe Ser Gly Xaa Ala 1 5 10 (2) Information of sequence identification number 14: (i) Sequence characteristics (A) Length: 12 amino acids (B) Type: Amino acid (C) Number of strands: Single strand (D) Form: Linear (ii) Molecular type: Peptide (xi) Sequence description: Sequence identification number No. 14 (TcdB N-terminus): Met Gin Asn Ser Gin Thr Phe Ser Val Gly Glu Leu 1 5 10 ⑵Sequence ID No. 15 Information: (i) Sequence characteristics: (A) Length: 14 amino acids (B) Type: Amino acid (C) Number of strands: Single strand (D) Form: Linear (ii) Molecular type: Peptide (xi) Sequence border: Sequence identification number 15 (TcaAii N-terminus): Ala Gin Asp Gly Asn Gin Asp Thr Phe Phe Ser Gly Asn Thr 1 5 10 (2) Serial Identification Number No. 16: (i) Sequence Features: (Please read the precautions on the back before filling this page) -3i6 —— This paper size applies to Chinese National Standard (CNS) A4 specification (210X 297 mm) 509722 A7 B7 V. Description of the invention (214) (A) Length: 5 amino acids (B) Type: amino acid (C ) Number of strands: Single strand (D) Form: Linear (ii) Molecular type: Peptide (xi) Sequence description: Sequence identification number 16 (TcbA N-terminus):

Met Gin Asn Ser Leu 1 5 (2) Information of sequence identification number 17: (i) Sequence characteristics: (A) Length: 10 amino acids (B) Type: amino acids (C) Number of shares: single Strand (D) Pattern: Linear (ii) Molecular Pattern: Peptide (xi) Sequence Description: Sequence Identification Number 17 (TcdAii-PTlll Internal Peptide): Ala Phe Asn He Asp Asp Val Ser Leu Phe 1 5 10 ( 2) Information of sequence identification number 18: (i) Sequence characteristics: (A) Length: 16 amino acids Amino acids (C) Number of strands: Single strand (D) Form: Linear (ii) Molecular type: Peptide (xi) sequence description: Sequence identification number 18 (TcdAii- PT79 internal peptide): Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (please read the precautions on the back before filling this page)

Phe He Val Tyr Thr Ser Leu Gly Val Asn Pro Asn Asn Ser Ser Asn 15 10 15 (2) Information of the sequence identification number 19: (i) Sequence characteristics: (A) Length: 21 amino acids (B) Type: Amino acid (C) Number of strands: Single strand (D) Form: Linear ⑹ Molecular type: Peptide 6d) Sequence description: Sequence identification number 19 (TcaBi- PT158 inner peptide) ·· This paper size applies China National Standard (CNS) A4 specification (210X297 mm) 509722 A7 B7 V. Description of invention (215)

He Ser Asp Leu Val Thr Thr Ser Pro Leu Ser Glu Ala lie Gly Ser 15 10 15 (Please read the notes on the back before filling this page)

Leu Gin Leu Phe lie 20 (2) Information of sequence identification number 20: (i) Sequence description: (A) Length: 12 amino acids (B) Type: amino acids (C) Number of shares: single strand (D) Form: Linear (ii) Molecular type: Peptide (xi) Sequence description: Sequence identification number 20 (TcaBi-P08 08 peptide): Met Tyr Tyr lie Gin Ala Gin Gin Leu Leu Gly Pro 1 5 10资料 Data of Serial Identification No. 21: (i) Sequence characteristics: (A) Length: 26 amino acids (B) Type: amino acids (C) Number of shares: single-share employee consumption cooperative of the Central Standards Bureau of the Ministry of Economic Affairs Printed (D) Form: Linear (ii) Molecular type: Peptide (xi) Sequence description: Sequence identification number 21 (TcbAii-P03 03 internal peptide): Gly He Asp Ala Val Leu Ser Met Glu Thr Gin Asn He Gin Glu Pro 15 10 15

Gin Leu Gly Ala Gly Thr Tyr Val Gin Leu 20 25 (2) Information of serial identification number 22: This paper size applies Chinese National Standard (CNS) A4 specification (210X297 mm) 509722 A7 B7 V. Description of the invention (216 ) (i) Sequence characteristics: (A) Length: 15 amino acids (B) Type: Amino acid (C) Number of strands: Single strand (D) Morphology: Linear (ii) Molecular type: Peptide (xi) Sequence description: Sequence identification number 22 (TcbAii- PT56 internal peptide): lie Ser Asn Pro lie Asn lie Asn Thr Gly He Asp Ser Ala Lys 15 10 15 资料 Data of sequence identification number 23: (i) Sequence Features: (A) Length: 13 amino acids (B) Type: Amino acid (C) Number of strands: Single strand (D) Form: Linear (ii) Molecular type: Peptide 6d) Sequence description ii: Sequence identification No. 23 (TcbA-PT81 (a) within the win): Thr Tyr Leu Thr Ser Phe Glu Gin Val Ala Asn Leu Lys 1 5 10 (Please read the notes on the back before filling this page) Set the central standard of the Ministry of Economic Affairs Printed by the Bureau's Consumer Cooperative (2) Serial Identification Number No. 24: (i) Sequence Features: (A) Length: 22 Amino Acids (B) Type ·. Amino Acids (C) Number of Shares: Single Strand (D) Form: Linear (ii) Molecular type: Peptide (xi) Sequence description: Sequence identification number 24 (TcbAi i-PT81 (b) Internal peptide): Val Leu Gly Thr Glu Asn Val He Ala Leu Tyr Ser Glu Asn Asn Gly 15 10 15

Val Gin Tyr Met Gin He This paper size is in accordance with Chinese National Standard (CNS) A4 (21〇 × 25? 1%) 509722 Printed by A7 B7 of the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 5. Description of the invention (217) 20 ( 2) Information of sequence identification number 25: (i) Sequence characteristics: (A) Length: 6054 base pairs (B) Type: Nucleic acid (C) Number of strands: Double strand (D) Form: Linear (ii) Molecular type: DNA (genome) (ix) Characteristics:

(A) Name / Tip: CDS (B) Position: 1. · 43 (D) Other information: / 产品 = " TcaAiii terminal " (ix) Specially:

(A) Name / Reminder: RBS (B) Location · 51 58 (ix) Features:

(A) Name / Tips: CDS (B) Location: 65 3634 (D) Other information: / Product = " TcaBi " (xi) Sequence description: Sequence ID No. 25: A GTA GCC CAA AAC m AGT GCC GCA ATC AGC AAT (UT CAG TAACCGGATA 50 Val Ala Gin Asn Leu Ser Ala Ala lie Ser Asn Arg Gin *** AAGAAGGAAT TGATT ATG TCT GAA TCT m ΤΓΤ ACA CAA ACG TTG AAA GAA 100 Met Ser Glu Ser Leu Phe Thr Gin Thr Leu Lys Glu 1 5 10 GCG CGC CGT GAT GCA TTG GTT GCT CAT TAT Απ GCT ACT CAG GTG CCC 148

Ala Arg Arg Asp Ala Leu Val Ala His Tyr lie Ala Thr Gin Val Pro 15 20 25 GCA GAT m AAA GAG AGT ATC CAG ACC GCG GAT GAT CTG TAC GAA TAT 196

Ala Asp Leu Lys Glu Ser He Gin Thr Ala Asp Asp Leu Tyr Glu Tyr 30 35 40 CTG TTG CTG GAT ACC ΜΑ Απ AGC GAT CTG GTT ACT ACT TCA CCG CTG 244

Leu Leu Leu Asp Thr Lys He Ser Asp Leu Val Thr Thr Ser Pro Leu 45 50 55 60 TCC GM GCG Απ GGC AGT CTG CM TTG TIT ATT CAT CGT GCG ATA GAG 292 -22Q- This paper is in accordance with China National Standards (CNS) A4 specifications (210X297 mm) --- 5 --------- (Please read the notes on the back before filling this page) Ordered by the Central Consumers Bureau of the Ministry of Economic Affairs and printed by the Consumer Cooperative 509722 A7 B7 V. Invention Instructions (218)

Ser Glu Ala He Gly Ser Leu Gin Leu Phe He His Arg Ala lie Glu 65 70 75 GGC TAT GAC GGC ACG CTG GCA GAC TCA GCA AAA CCC TAT ΤΓΤ GCC GAT 340

Gly Tyr Asp Gly Thr Leu Ala Asp Ser Ala Lys Pro Tyr Phe Ala Asp 80 85 90 GAA CAG ΤΓΤ m TAT AAC TGG GAT AGT TIT AAC CAC CGT TAT AGC ACT 388

Glu Gin Phe Leu Tyr Asn Trp Asp Ser Phe Asn His Arg Tyr Ser Thr 95 100 105 TGG GCT GGC MG GM CGG TTG AAA TTC TAT GCC GGG GAT TAT Απ GAT 436

Trp Ala Gly Lys Glu Arg Leu Lys Phe Tyr Ala Gly Asp Tyr He Asp 110 115 120 CCA ACA TCA CTGjX TA AAT AAG ACC GAG ΑΤΑ ΤΤΓ ACC GCA TIT GAA CM 484

Pro Thr Leu Arg Leu Asn Lys Thr Glu lie Phe Thr Ala Phe Glu Gin 125 130 135 140 GC5T ATT TCT CAA GGG AAA m AAA AGT GAA TTA GTC GAA TCT AAA m 532

Gly He Ser Gin Gly Lys Leu Lys Ser Glu Leu Val Glu Ser Lys Leu 145 150 155 CGT GAT TAT CTA Απ AGT TAT GAC ACT m GCC ACC CTT GAT TAT Απ 580

Arg Asp Tyr Leu lie Ser Tyr Asp Thr Leu Ala Thr Leu Asp Tyr lie 160 165 170 ACT GCC TGC CAA GGC AAA GAT AAT AM A (X ATC TTC m Απ GGC CGT 628

Thr Ala Cys Gin Gly Lys Asp Asn Lys Thr He Phe Phe lie Gly Arg 175 180 185 ACA CAG AAT GCA CCC TAT GCA TIT TAT TGG CGA AAA m ACT m GTC 676

Thr Gin Asn Ala Pro Tyr Ala Phe Tyr Trp Arg Lys Leu Thr Leu Val 190 195 200 ACT GAT GGC GGT AAG TTG AAA CCA GAT CAA TGG TCA GAG TGG CXjA GCA 724

Thr Asp Gly Gly Gly Lys Leu Lys Pro Asp Gin Trp Ser Glu Trp Arg Ala 205 210 215 220 ATT AAT GCC GGG ATT AC3T GAG GCA TAT TCA GGG CAT GTC GAG CCT HC 772

He Asn Ala Gly lie Ser Glu Ala Tyr Ser Gly His Val Glu Pro Phe 225 230 235 ___ 231 This paper size applies to China National Standard (CNS) A4 specification (210X297 mm) 丨 J .-------- ( Please read the notes on the back before filling this page), 1T 509722 A7 B7 V. Description of the invention (219) TGG GAA AAT AAC AAG CTO CAC AOT OT TCG TIT ACT ATC TCG AAA GAA 820

Trp Glu Asn Asn Lys Leu His He Arg Trp Phe Thr He Ser Lys Glu 240 245 250 GAT AAA ATA GAT m (ΠΤ TAT AM MC ATC TGG GTG ATG AGT AGC GAT 868

Asp Lys lie Asp Phe Val Tyr Lys Asn lie Trp Val Met Ser Ser Asp 255 260 265 TAT AGC TGG GCA TCA MG AAA AAA ATC TTG GM CIT TCT ΉACT ACT GAC 916

Tyr Ser Trp Ala Ser Lys Lys Lys He Leu Glu Leu Ser Phe Thr Asp 270 275 280 TAC AAT AGA GTT C3GA GCA ACA GGA TCA TCA AGC CCG ACT GAA GTA GCT 964

Tyr Asn Arg Val Gly Ala Thr Gly Ser Ser Ser Pro Thr Glu Val Ala 285 290 295 300 TCA CM TAT GGT TCT GAT GCT CAG ATG MT ATT TCT GAT GAT GGG ACT 1012

Ser Gin Tyr Gly Ser Asp Ala Gin Met Asn lie Ser Asp Asp Gly Thr 305 310 315 GTA CTT ATT TIT CAG MT GCC GGC GGA GCT ACT CCC AGT ACT GGA GTG 1060

Val Leu lie Phe Gin Asn Ala Gly Gly Ala Thr Pro Ser Thr Gly Val 320 325 330 ACG TTA TGT TAT GAC TCT GGC AAC GTG Απ AAG AAC CTA TCT AGT ACA 1108

Thr Leu Cys Tyr Asp Ser Gly Asn Val lie Lys Asn Leu Ser Ser Thr 335 340 345 GGA AGT GCA AAT TTA TCG TCA AAG GAT TAT GCC ACA ACT AAA TTA CX3C 1156

Gly Ser Ala Asn Leu Ser Ser Lys Asp Tyr Ala Thr Thr Lys Leu Arg 350 355 360 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling this page) ATG TGT CAT GGA CM AGT TAC AAT GAT AAT AAC TAC TGC AAT TTT ACA 1204

Met Cys His Gly Gin Ser Tyr Asn Asp Asn Asn Tyr Cys Asn Phe Thr 365 370 375 380 CTC TCT ATT AAT ACA ATA GAA TO ACC TCC TAC GGC ACA TTC TCA TCA 1252

Leu Ser lie Asn Thr lie Glu Phe Thr Ser Tyr Gly Thr Phe Ser Ser 385 390 395 GAT GGA AAA CM ΊΤΓ ACA CCA CCT TCT GGT TCT GCC ΚΠ GAT TTA CAC 1300 ___ This paper size applies to the Chinese National Standard (CNS) A4 specifications ( 210X 297 mm) 509722 A7 B7 V. Description of the invention (22〇)

Asp Gly Lys Gin Phe Thr Pro Pro Ser Gly Ser Ala lie Asp Leu His 400 405 410 CTC CCT AAT TAT GTA GAT CTC AAC GCG CTA m GAT Απ AGC CTC GAT 1348

Leu Pro Asn Tyr Val Asp Leu Asn Ala Leu Leu Asp He Ser Leu Asp 415 420 425 TCA CTA CTT AAT TAT GAC (ΠΤ CAG OiG CAG TIT GGC GGA TCT AAT CCG 1396

Ser Leu Leu Asn Tyr Asp Val Gin Gly Gin Phe Gly Gly Ser Asn Pro 430 435 440 GTT GAT AAT TTC ACT GGT CCC TAT GGT Απ TAT CTA TGG GAA ATC TTC 1444

Val Asp Asn Phe Ser Gly Pro Tyr Gly He Tyr Leu Trp Glu lie Phe 445 450 455 460 TTC CAT ATT CCG ITC CTT GTT ACG GTC CGT ATG CM ACC GM CAA CGT 1492

Phe His He Pro Phe Leu Val Thr Val Arg Met Gin Thr Glu Gin Arg 465 470 475 TAC GM GAC GCG GAC ACT TGG TAC AM TAT ATT TTC CGC AGC GCC GGT 1540

Tyr Glu Asp Ala Asp Thr Trp Tyr Lys Tyr lie Phe Arg Ser Ala Gly 480 485 490 TAT (XjC GAT GCT AAT GGC CAG CTC ATT ATG GAT GGC ACT AAA CCA CXjT 1588

Tyr Arg Asp Ala Asn Gly Gin Leu lie Met Asp Gly Ser Lys Pro Arg 495 500 505 TAT TGG AAT GTG ATG CCA TTG CM CTG GAT ACC GCA TGG GAT ACC ACA 1636

Tyr Trp Asn Val Met Pro Leu Gin Leu Asp Thr Ala Trp Asp Thr Thr 510 515 520 CAG CCC GCC ACC ACT GAT CCA GAT GTG ATC GCT ATG GCG GAC CCG ATG 1684 (Notes on the back, please fill out this page) d

Gin Pro Ala Thr Thr Asp Pro Asp Val He Ala Met Ala Asp Pro Met 525 530 535 540 CAT TAC AAG CTG GCG ATA TTC CTG CAT ACC CTT GAT CTA TTG Απ GCC 1732

His Tyr Lys Leu Ala He Phe Leu His Thr Leu Asp Leu Leu He Ala 545 550 555 CGA GGC GAC AGC GCT TAC CAA CTT GAA CGC GAT ACT CTA GTC GAA 1780

Arg Gly Asp Ser Ala Tyr Arg Gin Leu Glu Arg Asp Thr Leu Val Glu 560 565 570 ------ 333- This paper size applies to Chinese National Standard (CNS) A4 (210X 297 mm) 509722 A7 B7 V. Description of the invention (22 丨) GCC AAA ATG TAC TAC ΚΠ CAG GCA CM CAG CTA CTC GGA COG CGC CCT 1828

Ala Lys Met Tyr Tyr He Gin Ala Gin Gin Leu Leu Gly Pro Arg Pro 575 580 585 GAT ATC CAT ACC ACC AAT ACT ΤχΪ (XA MT (XC ACC ITG AGT AAA GAA 1876

Asp lie His Thr Thr Asn Thr Trp Pro Asn Pro Thr Leu Ser Lys Glu 590 595 600 GCT GGC GCT Απ GCC ACA OXi ACA TTC CTC AGT TCA CCG GAG GTG ATG 1924

Ala Gly Ala He Ala Thr Pro Thr Phe Leu Ser Ser Pro Glu Val Met 605 610 615 620 ACG TTC GCT GCC TGG CTA AGC GCA GGC GAT ACC GCA MT Απ GGC GAC 1972

Thr Phe Ala Ala Trp Leu Ser Ala Gly Asp Thr Ala Asn lie Gly Asp 625 630 635 GCT GAT TTC TTG CCA CCG TAC AAC GAT GTA CTA CTC GTC TAC TGG GAT 2020

Gly Asp Phe Leu Pro Pro Tyr Asn Asp Val Leu Leu Gly Tyr Trp Asp 640 645 650 AAA CIT GAG m CGC CTA TAC AAC CTG CGC CAC AAT CTG AGT CTG GAT 2068

Lys Leu Glu Leu Arg Leu Tyr Asn Leu Arg His Asn Leu Ser Leu Asp 655 660 665 GGT CM CCG CTA MT CTG CCA CTG TAT GCC AOG COG GTA GAC COG AAA 2116

Gly Gin Pro Leu Asn Leu Pro Leu Tyr Ala Thr Pro Val Asp Pro Lys 670 675 680 ACC CIO CAA CGC CAG CM GCC GGA GGG GAC GGT ACA GGC AGT AGT (XG 2164

Thr Leu Gin Arg Gin Gin Ala Gly Gly Asp Gly Thr Gly Ser Ser Pro Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economy 685 690 695 700 GCT GGT GGT CAA GGC AGT GTT CAG GGC TGG CGC TAT COG ΤΓΑ TTG GTA 2212

Ala Gly Gly Gin Gly Ser Val Gin Gly Trp Arg Tyr Pro Leu Leu Val 705 710 715 GAA CGC GCC CGC TCT GCC GTG AGT TTG TTG ACT CAG TTC GGC MC AGC 2260

Glu Arg Ala Arg Ser Ala Val Ser Leu Leu Thr Gin Phe Gly Asn Ser 720 725 730-^ 224-— This paper size applies to China National Standard (CNS) A4 (210X 297 mm) Employees of the Central Standards Bureau of the Ministry of Economic Affairs Printed by the cooperative 509722 A7 B7 V. Description of the invention (222) m CM ACA ACG m GAA CAT CAG GAT AAT GAA AAA ΑΊΌ AOG ATA CTG 2308

Leu Gin Thr Thr Leu Glu His Gin Asp Asn Glu Lys Met Thr lie Leu 735 740 745 TTG CAG ACT CM CAG GAA GCC ATC CTG AAA CAT CAG CAC GAT ATA CM 2356

Leu Gin Thr Gin Gin Glu Ala He Leu Lys His Gin His Asplie Gin 750 755 760 CAA AAT AAT CTA AAA GGA m CM CAC AGC CTG ACC GCA TTA CAG GCT 2404

Gin Asn Asn Leu Lys Gly Leu Gin His Ser Leu Thr Ala Leu Gin Ala 765 770 775 780 AGC CGT GAT GGC GAC ACA TTG CGG CAA AAA CAT TAC AGC GAC CTG Απ 2452

Ser Arg Asp Gly Asp Thr Leu Arg Gin Lys His Tyr Ser Asp Leu He 785 790 795 MC GGT GGT CTA TCT GCG GCA GAA ATC GCC GGT CTG ACA CTA CGC AGC 2500

Asn Gly Gly Leu Ser Ala Ala Glu He Ala Gly Leu Thr Leu Arg Ser 800 805 810 ACC GCC ATC ATT ACC MT GGC GTT GCA AOG GGA TTG CTG ΑΠ GCC GGC 2548

Thr Ala Met lie Thr Asn Gly Val Ala Thr Gly Leu Leu He Ala Gly 815 820 825 GGA ATC GCC MC GCG GTA CCT MC GTC TTC GGG CTG GCT MC GGT GGA 2596

Gly He Ala Asn Ala Val Pro Asn Val Phe Gly Leu Ala Asn Gly Gly 830 835 840 TCG GAA TGG GGA GOG CCA m ATT GGC TCT C3GG CAA GCA ACC CAA GTT 2644

Ser Glu Trp Gly Ala Pro Leu He Gly Ser Gly Gin Ala Thr Gin Val 845 850 855 860 GGC GCC GGC ATC CAG GAT CAG AGC GOj GGC Απ TCA GAA GTG ACA GCA 2692

Gly Ala Gly lie Gin Asp Gin Ser Ala Gly lie Ser Glu Val Thr Ala 865 870 875

OjC TAT CAG CXjT ΟΪΤ CAG GAA GAA TGG CjCA TTG CAA CGG GAT ATT GCT 2740

Gly Tyr Gin Arg Arg Gin Glu Glu Trp Ala Leu Gin Arg Asp He Ala 880 885 890 GAT AAC GAA ATA ACC CAA CTG GAT GCX: CAG ATA CAA AGC CTG CAA GAG 2788

Asp Asn Glu He Thr Gin Leu Asp Ala Gin lie Gin Ser Leu Gin Glu __ This paper size applies to China National Standard (CNS) A4 specification (210X 297 mm) (Please read the precautions on the back before filling this page)

509722 A7 B7 Five Invention Description (223) 895 900 905 CM ATC ACXj ATG GCA CM AAA CAG ATC ACG CTC TCT GAA ACC GAA CM 2836

Gin He Thr Met Ala Gin Lys Gin lie Thr Leu Ser Glu Thr Glu Gin 910 915 920 GCG MT GCC CM GCG ATT TAT GAC CTG CM ACC ACT CGT ΤΤ ACC GGG 2884

Ala Asn Ala Gin Ala He Tyr Asp Leu Gin Thr Thr Arg Phe Thr Gly 925 930 935 940 CAG GCA CTG TAT MC TGG ATG GCC GGT CGT CTC TCC GOG CTC TAT TAC 2932

Gin Ala Leu Tyr Asn Trp Met Ala Gly Arg Leu Ser Ala Leu Tyr Tyr 945 950 955 CM ATG TAT GAT TCC ACT CTG CCA ATC TGT CTC CAG CCA MA GCC GCA 2980

Gin Met Tyr Asp Ser Thr Leu Pro He Cys Leu Gin Pro Lys Ala Ala 960 965 970 m GTA CAG GAA m GGC GAG AAA GAG AGC GAC ACTT CTT TTC CAG GTT 3028

Leu Val Gin Glu Leu Gly Glu Lys Glu Ser Asp Ser Leu Phe Gin Val 975 980 985 CCG GTG TGG MT GAT CTG TGG CM GGG CTG m GCA GGA GAA GGT m 3076

Pro Val Trp Asn Asp Leu Trp Gin Gly Leu Leu Ala Gly Glu Gly Leu 990 995 1000 AGT TCA GAG CTA CAG AAA CTG GAT GCC ATC TGG CTT GCA CGT GGT GGT 3124

Ser Ser Glu Leu Gin Lys Leu Asp Ala lie Trp Leu Ala Arg Gly Gly 1005 1010 1015 1020 ATT GGG CTA GM GCC ATC CGC ACC GTG TCG CTG GAT ACC CTG ΉΤ GGC 3172

He Gly Leu Glu Ala lie Arg Thr Val Ser Leu Asp Thr Leu Phe Gly 1025 1030 1035 ACA GGG ACG TTA AGT GAA MT ATC MT AM GTC OT AAC GGG GAA ACG 3220

Thr Gly Thr Leu Ser Glu Asn lie Asn Lys Val Leu Asn Gly Glu Thr 1040 1045 1050 GTA TCT CCA TCC GGT GGC GTC ACT CTG GCG CTG ACA GGG GAT ATC TTC 3268

Val Ser Pro Ser Gly Gly Val Thr Leu Ala Leu Thr Gly Asp He Phe 1055 1060 1065 This paper size is applicable to the Chinese National Standard (CNS) A4 size (210X297 mm) Please read the notes on the back first 1! J binding economy Printed by the Consumer Standards Cooperative of the Ministry of Standards of the People's Republic of China 509722 Printed by the Consumer Standards Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs A7 B7 V. Invention Description (224) CM GCA ACA CTG GAT TTG AGT CAG CTA GGT TTG GAT AAC TCT TAC AAC 3316 Gin Ala Thr Leu Asp Leu Ser Gin Leu Gly Leu Asp Asn Ser Tyr Asn 1070 1075 1080 TTG GGT MC GAG MG AAA CGT CGT Απ AAA CGT ATC GCC GTC ACC CTG 3364 Leu Gly Asn Glu Lys Lys Arg Arg lie Lys Arg He Ala Val Thr Leu 1085 1090 1095 1100 CCA ACA CIT CTG GGG CCA TAT CM GAT CTT GAA GCC ACA CTG GTA ATG 3412 Pro Thr Leu Leu Gly Pro Tyr Gin Asp Leu Glu Ala Thr Leu Val Met 1105 1110 1115 GGT GCG GAA ATC GCC GCC TTA TCA CAC GGT GTGAT GAC GGA GGC CGG 3460 Gly Ala Glu lie Ala Ala Leu Ser His Gly Val Asn Asp Gly Gly Arg 1120 1125 1130 ΉΤ GIT ACC GAC ΊΤ MC GAC AGC GGT TTT CTG CCT ΤΊ GM GGT OGA 3508 Phe Val Thr Asp Phe Asn Asp Ser Arg Phe Leu Pro Phe Glu Gly Arg 1135 1140 1145 GAT GCA ACA ACC GGC ACA CTG GAG CTC MT ATT TTC CAT GCG GGT AAA 3556 Asp Ala Thr Thr Gly Thr Leu Glu Leu Asn He Phe His Ala Gly Lys 1150 1155 1160 GAG GGA ACG CM CAC GAG ΉΌ GTG GCG AAT CTG AGT GAC ATC ATT GTG 3604 Glu Gly Thr Gin His Glu Leu Val Ala Asn Leu Ser Asp lie He Val 1165 1170 1175 1180 CAT CTG MT TAC ATC Απ CGA GAC GCG TM ATTIUmTC TTTGTCGATT 3654 His Leu Asn Tyr lie lie Arg Asp Ala * 1185 1190 ACAGGTCCCT ATCAGGGGCC TdnmkkG GAGTACITTA TGCAGGATTC ACCAGAAGTA 3714 TCXjATTACAA OOGTCACT TCCCAAAGGT GCmjTGCTA TCAATGGCAT GGGAGAAGCA 3774 CTGAATGCTG CCXjGCCCTGA TGGAATGGCC TCCCTATCTC TGCCAmCX: CCTTTCGACC 3834 GGCAGAGGGA (WCTCCYQG AmTCGCTG ATTTACAGCA ACAGTGCAGG TAATGGGCCT 3894 TTCGGCATCG GCTGGCMTG CGGTGTTATG TCCAmGCC GACGCACCCA ACATGGCATT 3954 (Please read the precautions on the back before filling this page).

, 1T

—----- J3XU— This paper size applies to China National Standard (CNS) A4 (210X 297 mm) Printed by the Consumer Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs 509722 A7 B7 V. Description of Invention (225) GCXjAGGTCAT GAATATCGCC 4014 CTGAATGACC AAGGGCAACC TGATATCOTr CAAGACC7ITA AMCGCTGCA AGGOGTTACC 4074 TTGCCAATTT CCTATACCGT GACCCGCTAT CAAGCCXXKX: AGATCCTGGA TTTCAGTAAA 4134 ATCXjAATACT GGCAACCTGC CTCXXJGTCAA GAAGGACGCXj CTTTCTRjCT GATATCGACA 4194 CCGGACGGGC ATCTACACAT CITAGGGAAA ACOGCGCAGG CTTGTCTGGC AAATCCGCM 4254 AATGACCAAC AAATCXjCCCA ϋΊΧΧΠΤΧΚΠΌ GAAGAAACTG TGACGCCAGC CGGTGAACAT 4314 GTCAGCTATC AATATCGAGC CXjAAGATGAA (m: ATKjTG AOGACAATGA AAAAACXX5CT 4374 CATCCTAATG mCCGCACA GCXjCTATCrG GTACAGGTGA ACTACAGGCA ACATCAAAOC 4434 ACAAGCCAGC CTXjTTCGTAC TGGATAACGC ACCTCCOGCA CCGGAAGAGT OOGTITCA 4494 TCTXKjTCITTC GACCACGGTCTCATCGGATACGGGAGAGCAG GCTTATC TCMCMGTG CTGATGTTTC AC0GCA00GC GCTCATGGCC 4674 GGAGAAGCCA GTACCAATGA CGCCCXX3GAA OXXjTTGGAC GCTTAATACT GGAATATGAC 4634 AAAAACGCCA GCGTCACCAC GTTGAmCC ATCCGTCAAT TAAGCCATGA ATCXjGACGGG 4794 AGGCCAGTCA CCCAGCCACC ACTAGAACTA GCTTGGCAAC GGTITGATCr GGAGAAAATC 4854 CXXjACATGGC MCGCTTTGA CGCACTAGAT AATHTAACT CXjCAGCAACXj mTCAACTG 4865 (HTCATCTCC GGGGAGAAGG CjITGCCAGGT ATGCTGTATC AAGATCGAGG OGCTTCGTGG 4914 TATAAAGCTC OJCAACGTCA GGAAGACGGA GACAGCAATG COGTCACTTA CGACAAAATC 4974 GOrCACTGC CTACCCTACC CAATITGCAG GATAATGCCT CATKJATGGA TATCAACGGA 5034 GACGGCCAAC TOATTGGGT TCTTACCXjCC TCCGGTAnC GCGGATACCA TAGTCAGCAA 5094 CCCGATGGAA AGTGGACGCA CITTACGCCA ATCAATGCCT TXmXJTGGA ATATHTCAT 5-20 --- Applicable to China Standards-210-m Please fill in this page for further information)-Packing. Order printed by the Consumer Cooperatives of the Central Bureau of Standards of the Ministry of Economic Affairs 509722 A7 B7 _ V. Description of Invention (226) CCGAAAAGCG TGCGTCTATA TGCCAACCAG CXJAAA0GGCT GGCGTAAAGG AGAAGATGTC 5334 CCCCMTOCA CAGGTATCAC CCTGCCTGTC ACAGGGACCG ATGCCCGCM ACTGGTGGCT 5394 TTCAGTGATA TCCTCGGITC CGC5TCAACAA CATOGGTGG AAATCAAGGG TAATCX3CGTC 5454 A (riUrTGGC CXjAATCTAGG GCATXjCjCCGT TTGGGTCAAC CACTAACTCT GTCAGGATIT 5514 AGCCAGCCCG AAAATAGCTT CMTCOCGM CGGCTGTTTC TGGCGGATAT OGACGGCTGC 5574 GGCACCACCG ΑΟΧΠΤΑΊϋΓΑ TGCGCAATCC GCOCTTKX: TCAmATUT CAACCAAAGT 5634 GGTAATCAGT TTGATGCCCC GTTGACATTA GCGTTGCCAG AAGGCGTACA ATTTGACAAC 5694 ACTTGCCAAC TTCAAGTCGC CGATAHCAG GGAmGGGA TACjCCAGCTT GATTCTGACT 5754 GTGCCACATA TCGCGCCACA TCACTGGCGT TGTGACCTGT CACTGACCM ACCCTGGTrG 5814 TTGAATGTAA TGAACAATAA OHjGGCGCA CATCACAGGC TACAmTOG TAGTTCOGCG 5874 CAATTCTGGT TGGATGAAAA ATTACAGCTC ACCAAAGCAG GCAAATCTCC OjCTTGTTAT 5934 CTGCCGTITC CAATGCATTT GCTATGGTAT ACOGAAATTC AGGATGMAT CAGCGGCMC 5994 CGGCTCACCA GTGMGTCM CTACAGCCAC GGCGTCTGGG ATGGTAAAGA GCGGGMTTC 6054 ⑵ sequence identification number 26 No. of information: (i) Sequence characteristics: (A) Length: 118 9 amino acids (B) type: amino acid (D) form: linear (ii) molecular type · protein (xi) sequence description: sequence identification number 26 (TcaB protein):

Met Ser Glu Ser Leu Phe Thr Gin Thr Leu Lys Glu Ala Arg Arg Asp 15 10 15

Ala Leu Val Ala His Tyr He Ala Thr Gin Val Pro Ala Asp Leu Lys -_____ This paper size applies to China National Standard (CNS) A4 size (210X 297 mm) (Please read the precautions on the back before filling this page) • Loading ·

1T 509722 A7 B7 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs V. Invention Description (227) 20 25 30 Glu Ser lie Gin Thr Ala Asp Asp Leu Tyr Glu Tyr Leu Leu Leu Asp 35 40 45 Thr Lys lie Ser Asp Leu Val Thr Thr Ser Pro Leu Ser Glu Ala lie 50 55 60 Gly Ser Leu Gin Leu Phe lie His Arg Ala lie Glu Gly Tyr Asp Gly 65 70 75 80 Thr Leu Ala Asp Ser Ala Lys Pro Tyr Phe Ala Asp Glu Gin Phe Leu 85 90 95 Tyr Asn Trp Asp Ser Phe Asn His Arg Tyr Ser Thr Trp Ala Gly Lys 100 105 110 Glu Arg Leu Lys Phe Tyr Ala Gly Asp Tyr lie Asp Pro Thr Leu Arg 115 120 125 Leu Asn Lys Thr Glu lie Phe Thr Ala Phe Glu Gin Gly lie Ser Gin 130 135 140 Gly Lys Leu Lys Ser Glu Leu Val Glu Ser Lys Leu Arg Asp Tyr Leu 145 150 155 160 lie Ser Tyr Asp Thr Leu Ala Thr Leu Asp Tyr lie Thr Ala Cys Gin 165 170 175 Gly Lys Asp Asn Lys Thr lie Phe Phe lie Gly Arg Thr Gi n Asn Ala 180 185 190 Pro Tyr Ala Phe Tyr Trp Arg Lys Leu Thr Leu Val Thr Asp Gly Gly 195 200 205 Lys Leu Lys Pro Asp Gin Trp Ser Glu Trp Arg Ala lie Asn Ala Gly 210 215 220 lie Ser Glu Ala Tyr Ser Gly His Val Glu Pro Phe Trp Glu Asn Asn 225 230 235 240? 30-This paper size applies to China National Standard (CNS) A4 specification (210X 297 mm) 509722 A7 B7 V. Description of the invention (228)

Lys Leu His He Arg Trp Phe Thr He Ser Lys Glu Asp Lys lie Asp 245 250 255

Phe Val Tyr Lys Asn lie Trp Val Met Ser Ser Asp Tyr Ser Trp Ala 260 265 270

Ser Lys Lys Lys lie Leu Glu Leu Ser Phe Thr Asp Tyr Asn Arg Val 275 280 285

Gly Ala Thr Gly Ser Ser Ser Pro Thr Glu Val Ala Ser Gin Tyr Gly 290 295 300

Ser Asp Ala Gin Met Asn lie Ser Asp Asp Gly Thr Val Leu lie Phe 305 310 315 320

Gin Asn Ala Gly Gly Ala Thr Pro Ser Thr Gly Val Thr Leu Cys Tyr 325 330 335

Asp Ser Gly Asn Val lie Lys Asn Leu Ser Ser Thr Gly Ser Ala Asn 340 345 350

Leu Ser Ser Lys Asp Tyr Ala Thr Thr Lys Leu Arg Met Cys His Gly 355 360 365

Gin Ser Tyr Asn Asp Asn Asn Tyr Cys Asn Phe Thr Leu Ser He Asn 370 375 380

Thr He Glu Phe Thr Ser Tyr Gly Thr Phe Ser Ser Asp Gly Lys Gin 385 390 395 400

Phe Thr Pro Pro Ser Gly Ser Ala lie Asp Leu His Leu Pro Asn Tyr 405 410 415 Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs

Val Asp Leu Asn Ala Leu Leu Asp He Ser Leu Asp Ser Leu Leu Asn 420 425 430

Tyr Asp Val Gin Gly Gin Phe Gly Gly Ser Asn Pro Val Asp Asn Phe 435 440 445

Ser Gly Pro Tyr Gly He Tyr Leu Trp Glu He Phe Phe His He Pro 450 455 460 " 1 1 ........... This paper size applies to China National Standard (CNS) A4 (210X 297 (Mm) Printed by the Consumer Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs 509722 A7 B7 V. Description of Invention (229)

Phe Leu Val Thr Val Arg Met Gin Thr Glu Gin Arg Tyr Glu Asp Ala 465 470 475 480

Asp Thr Trp Tyr Lys Tyr He Phe Arg Ser Ala Gly Tyr Arg Asp Ala 485 490 495

Asn Gly Gin Leu He Met Asp Gly Ser Lys Pro Arg Tyr Trp Asn Val 500 505 510

Met Pro Leu Gin Leu Asp Thr Ala Trp Asp Thr Thr Gin Pro Ala Thr 515 520 525

Thr Asp Pro Asp Val lie Ala Met Ala Asp Pro Met His Tyr Lys Leu 530 535 540

Ala He Phe Leu His Thr Leu Asp Leu Leu He Ala Arg Gly Asp Ser 545 550 555 560

Ala Tyr Arg Gin Leu Glu Arg Asp Thr Leu Val Glu Ala Lys Met Tyr 565 570 575

Tyr lie Gin Ala Gin Gin Leu Leu Gly Pro Arg Pro Asp He His Thr 580 585 590

Thr Asn Thr Trp Pro Asn Pro Thr Leu Ser Lys Glu Ala Gly Ala lie 595 600 605

Ala Thr Pro Thr Phe Leu Ser Ser Pro Glu Val Met Thr Phe Ala Ala 610 615 620

Trp Leu Ser Ala Gly Asp Thr Ala Asn He Gly Asp Gly Asp Phe Leu 625 630 635 640

Pro Pro Tyr Asn Asp Val Leu Leu Gly Tyr Trp Asp Lys Leu Glu Leu 645 650 655

Arg Leu Tyr Asn Leu Arg His Asn Leu Ser Leu Asp Gly Gin Pro Leu 660 665 670

Asn Leu Pro Leu Tyr Ala Thr Pro Val Asp Pro Lys Thr Leu Gin Arg 675 680 685 _________ 2 ^ 2-This paper size applies to China National Standard (CNS) A4 specifications (210X 297 mm) (Please read the precautions on the back first Fill out this page again). Packing-509722 A7 B7 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs V. Invention Description (230) Gin Gin Ala Gly Gly Asp Gly Thr Gly Ser Ser Pro Ala Gly Gly Gin 690 695 700 Gly Ser Val Gin Gly Trp Arg Tyr Pro Leu Leu Val Glu Arg Ala Arg 705 710 715 720 Ser Ala Val Ser Leu Leu Tu Gin Phe Gly Asn Ser Leu Gin Thr Thr 725 730 730 Leu Glu His Gin Asp Asn Glu Lys Met Thr He Leu Leu Gin Thr Gin 740 745 750 Gin Glu Ala lie Leu Lys His Gin His Asp lie Gin Gin Asn Asn Leu 755 760 765 Lys Gly Leu Gin His Ser Leu Thr Ala Leu Gin Ala Ser Arg Asp Gly 770 775 780 Asp Thr Leu Arg Gin Lys His Tyr Ser Asp Leu lie Asn Gly Gly Leu 785 790 795 800 Ser Ala Ala Glu lie Ala Gly Leu Thr Leu Arg Ser Thr Ala Met lie 805 810 815 Thr Asn Gly Val Ala Thr Gly Leu Leu lie Ala Gly Gly lie Ala Asn 820 825 830 Ala Val Pro Asn Val Phe Gly Leu Ala Asn Gly Gly Ser Glu Trp Gly 835 840 845 Ala Pro Leu lie Gly Ser Gly Gin Ala Thr Gin Val Gly Ala Gly lie 850 855 860 Gin Asp Gin Ser Ala Gly lie Ser Glu Val Thr Ala Gly Tyr Gin Arg 865 870 875 880 Arg Gin Glu Glu Trp Ala Leu Gin Arg Asp lie Ala Asp Asn Glu lie 885 890 895 Thr Gin Leu Asp Ala Gin lie Gin Ser Leu Gin Glu Gin lie Thr Met 900 905 910 .733-This paper size applies to China National Standard (CNS) A4 (210X 297 mm) Ministry of Economic Affairs Printed by the Consumer Standards Cooperative of the Central Bureau of Standards 509722 A7 B7 V. Description of Invention (231)

Ala Gin Lys Gin lie Thr Leu Ser Glu Thr Glu Gin Ala Asn Ala Gin 915 920 925

Ala He Tyr Asp Leu Gin Thr Thr Arg Phe Thr Gly Gin Ala Leu Tyr 930 935 940

Asn Trp Met Ala Gly Arg Leu Ser Ala Leu Tyr Tyr Gin Met Tyr Asp 945 950 955 960

Ser Thr Leu Pro He Cys Leu Gin Pro Lys Ala Ala Leu Val Gin Glu 965 970 975

Leu Gly Glu Lys Glu Ser Asp Ser Leu Phe Gin Val Pro Val Trp Asn 980 985 990

Asp Leu Trp Gin Gly Leu Leu Ala Gly Glu Gly Leu Ser Ser Glu Leu 995 1000 1005

Gin Lys Leu Asp Ala He Trp Leu Ala Arg Gly Gly He Gly Leu Glu 1010 1015 1020

Ala lie Arg Thr Val Ser Leu Asp Thr Leu Phe Gly Thr Gly Thr Leu 1025 1030 1035 1040

Ser Glu Asn He Asn Lys Val Leu Asn Gly Glu Thr Val Ser Pro Ser 1045 1050 1055

Gly Gly Val Thr Leu Ala Leu Thr Gly Asp lie Phe Gin Ala Thr Leu 1060 1065 1070

Asp Leu Ser Gin Leu Gly Leu Asp Asn Ser Tyr Asn Leu Gly Asn Glu 1075 1080 1085

Lys Lys Arg Arg He Lys Arg He Ala Val Thr Leu Pro Thr Leu Leu 1090 1095 1100

Gly Pro Tyr Gin Asp Leu Glu Ala Thr Leu Val Met Gly Ala Glu He 1105 1110 1115 1120

Ala Ala Leu Ser His Gly Val Asn Asp Gly Gly Arg Phe Val Thr Asp 1125 1130 1135 This paper size is applicable to China National Standard (CNS) A4 size (210X 297 mm) (Please read the notes on the back first ^^ Writing Page) Equipment, τ 509722 A7 B7 V. Description of the invention (232)

Phe Asn Asp Ser Arg Phe Leu Pro Phe Glu Gly Arg Asp Ala Thr Thr 1140 1145 1150

Gly Thr Leu Glu Leu Asn He Phe His Ala Gly Lys Glu Gly Thr Gin 1155 1160 1165

His Glu Leu Val Ala Asn Leu Ser Asp He He Val His Leu Asn Tyr 1170 1175 1180 lie lie Arg Asp Ala * 1185 1190 资料 Sequence ID No. 27: (i) Sequence characteristics: (A) Length: 1881 Base-pair nucleic acid (C) strand number: double strand (D) type: linear (ii) molecular type: DNA (gene body) < ix) characteristics:

(A) Name / Title: CDS (B) Position: 1..1881 (D) Other information: tcaBi (xi) Sequence description: Sequence identification number 27 (tcaBi coding area): ATC TCT GM TCT m ΉΤ ACA CM ACG TTG AAA GM GCG CGC CGT GAT 48 Met Ser Glu Ser Leu Phe Thr Gin Thr Leu Lys Glu Ala Arg Arg Asp 15 10 15 Printed by GCA TTG GTT GCT CAT TAT Απ GCT ACT CAG GTG CCC GCA GAT TTA AAA 96

Ala Leu Val Ala His Tyr He Ala Thr Gin Val Pro Ala Asp Leu Lys 20 25 30 GAG AGT ATC CAG ACC GCG GAT GAT CTG TAC GAA TAT CTG TTG CTG GAT 144

Glu Ser lie Gin Thr Ala Asp Asp Leu Tyr Glu Tyr Leu Leu Leu Asp 35 40 45 ACC AAA Απ AGC GAT CTG GTT ACT ACT TCA CCG CTG TCC GM GCG Απ 192

Thr Lys lie Ser Asp Leu Val Thr Thr Ser Pro Leu Ser Glu Ala lie ___ This paper size applies to Chinese National Standard (CNS) A4 (210 X 297 mm) Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 509722 A7 B7_ 5 Description of the invention (233) 50 55 60 GGC AGT CTG CM TTG TIT ATT CAT CGT GCG ATA GAG GGC TAT GAC GGC 240

Gly Ser Leu Gin Leu Phe lie His Arg Ala He Glu Gly Tyr Asp Gly 65 70 75 80 ACG CTG GCA GAC TCA GCA AAA (XC TAT ΊΤΓ GCC GAT GAA CAG TIT m 288

Thr Leu Ala Asp Ser Ala Lys Pro Tyr Phe Ala Asp Glu Gin Phe Leu 85 90 95 TAT AAC TGG GAT AGT TIT AAC CAC OGT TAT AO: ACT TGG GCT GGC AAG 336

Tyr Asn Trp Asp Ser Phe Asn His Arg Tyr Ser Thr Trp Ala Gly Lys 100 105 110 GAA OGG TTG AAA TTC TAT GCC GGG GAT TAT ATT GAT CCA ACA TTG OGA 384

Glu Arg Leu Lys Phe Tyr Ala Gly Asp Tyr lie Asp Pro Thr Leu Arg 115 120 125 TTG AAT AAG ACC GAG ΑΤΑ ΉΤ ACT GCA TIT GAA CM GGT Απ TCT CAA 432

Leu Asn Lys Thr Glu lie Phe Thr Ala Phe Glu Gin Gly lie Ser Gin 130 135 140 GGG AAA TTA AAA AGT GAA m GTC GAA TCT AAA m CGT GAT TAT CTA 480

Gly Lys Leu Lys Ser Glu Leu Val Glu Ser Lys Leu Arg Asp Tyr Leu 145 150 155 160 ATT AGT TAT GAC ACT TTA GCC ACC CIT GAT TAT Απ ACT GCC TGC CAA 528 lie Ser Tyr Asp Thr Leu Ala Thr Leu Asp Tyr He Thr Ala Cys Gin 165 170 175 GGC AAA GAT AAT AAA ACC ATC TTC ΉΤ Απ GGC CGT ACA CAG AAT GCA 576

Gly Lys Asp Asn Lys Thr lie Phe Phe He Gly Arg Thr Gin Asn Ala 180 185 190 CCC TAT GCA TIT TAT TGG OGA AAA m ACT TTA GTC ACT GAT GGC GGT 624

Pro Tyr Ala Phe Tyr Trp Arg Lys Leu Thr Leu Val Thr Asp Gly Gly 195 200 205 AAG TTG AAA CCA GAT CAA TGG TCA GAG TGG CGA GCA Απ AAT GCC CX3G 672

Lys Leu Lys Pro Asp Gin Trp Ser Glu Trp Arg Ala He Asn Ala Gly 210 215 220 __- 236- This paper size applies to China National Standard (CNS) A4 specifications (210X 297 mm) (Please read the precautions on the back before (Fill in this page)

、 1T 509722 A7 B7 V. Description of the invention (234) ATT AGT GAG GCA TAT TCA GGG CAT GTC GAG CCT TTC TGG GAA AAT AAC 720

He Ser Glu Ala Tyr Ser Gly His Val Glu Pro Phe Trp Glu Asn Asn 225 230 235 240 MG CTG CAC ATC CGT TGG ΤΤACT ACT ATC TCG AAA GM GAT AAA ATA GAT 768

Lys Leu His lie Arg Trp Phe Thr lie Ser Lys Glu Asp Lys lie Asp 245 250 255 m GTT TAT AM MC ATC IXJG GTG ATG AGT AGC GAT TAT AGC TGG GCA 816

Phe Val Tyr Lys Asn He Trp Val Met Ser Ser Asp Tyr Ser Trp Ala 260 265 270 Τα MG AM AM ATC TO GM CTT TUT ΉACT ACT GAC TAC AAT AGA GTT 864

Ser Lys Lys Lys He Leu Glu Leu Ser Phe Thr Asp Tyr Asn Arg Val 275 280 285 GGA GCA ACA GGA TCA TCA AGC CCXJ ACT GAA GTA GCT TCA CM TAT GGT 912

Gly Ala Thr Gly Ser Ser Ser Pro Thr Glu Val Ala Ser Gin Tyr Gly 290 295 300 TCT GAT GCT CAG ATG MT ATT TCT GAT GAT GGG ACT GTA CTT ATT ΉΤ 960

Ser Asp Ala Gin Met Asn He Ser Asp Asp Gly Thr Val Leu He Phe 305 310 315 320 CAG AAT GCC GGC GGA GCT ACT CCC AGT ACT GGA GTG AOG m TGT TAT 1008

Gin Asn Ala Gly Gly Ala Thr Pro Ser Thr Gly Val Thr Leu Cys Tyr 325 330 335 GAC TCT GGC AAC GTG Απ AAG AAC CTA TCT AGT ACA GGA AGT GCA AAT 1056

Asp Ser Gly Asn Val He Lys Asn Leu Ser Ser Thr Gly Ser Ala Asn 340 345 350 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling this page) ΤΓΑ TCG TCA AAG GAT TAT GCC ACA ACT AAA m CGC ATG TGT CAT GGA 1104

Leu Ser Ser Lys Asp Tyr Ala Thr Thr Lys Leu Arg Met Cys His Gly 355 360 365 CAA AGT TAC AAT GAT AAT AAC TAC TGC AAT TIT ACA CTC TCT Απ AAT 1152

Gin Ser Tyr Asn Asp Asn Asn Tyr Cys Asn Phe Thr Leu Ser He Asn 370 375 380 ACA ATA GM TTC ACC TOC TAC GGC ACA TTC TCA TCA GAT GGA AAA CM 1200

Thr lie Glu Phe Thr Ser Tyr Gly Thr Phe Ser Ser Asp Gly Lys Gin ____- 237- This paper size applies to Chinese National Standard (CNS) A4 specification (210X297 mm) 509722 A7 B7 V. Description of the invention 385 235 390 395 400 TIT ACA CCA CCT TCT GGT TCT GOC ATT GAT m CAC CTC CCT MT TAT 1248

Phe Thr Pro Pro Ser Gly Ser Ala lie Asp Leu His Leu Pro Asn Tyr 405 410 415 GTA GAT CTC AAC GCG CTA ΤΓΑ GAT ATT AGC CTC GAT TCA CTA CTT AAT 1296

Val Asp Leu Asn Ala Leu Leu Asp lie Ser Leu Asp Ser Leu Leu Asn 420 425 430 TAT GAC GTT CAG GGG CAG ΤΊΤ GGC GGA TCT MT CCG GTT GAT MT TTC 1344

Tyr Asp Val Gin Gly Gin Phe Gly Gly Ser Asn Pro Val Asp Asn Phe 435 440 445 AC3T GGT (XC TAT GGT ATT TAT CTA TGG GAA ATC TTC WC CAT ATT CCG 1392

Ser Gly Pro Tyr Gly lie Tyr Leu Trp Glu He Phe Phe His lie Pro 450 455 460 TTC CTT GTT ACG GTC CGT ATG CM ACC GM CM CGT TAC GAA GAC GCG 1440

Phe Leu Val Thr Val Arg Met Gin Thr Glu Gin Arg Tyr Glu Asp Ala 465 470 475 480 GAC ACT TGG TAC AAA TAT ATTTTC CGC AGC GCC GGT TAT CGC GAT GCT 1488

Asp Thr Trp Tyr Lys Tyr He Phe Arg Ser Ala Gly Tyr Arg Asp Ala 485 490 495 AAT GGC CAG CTC ATT ATG GAT GGC AGT AAA CCA CGT TAT TGG AAT GTG 1536

Asn Gly Gin Leu lie Met Asp Gly Ser Lys Pro Arg Tyr Trp Asn Val 500 505 510 ATG CCA TTG CAA CTG GAT ACC CjCA TGG GAT ACC ACA CAG CCC GCC ACC 1584

Met Pro Leu Gin Leu Asp Thr Ala Trp Asp Thr Thr Gin Pro Ala Thr 515 520 525 ACT GAT CCA GAT GTG ATC GCT ATG GCG GAC (TG ATG CAT TAC AAG CTG 1632

Thr Asp Pro Asp Val lie Ala Met Ala Asp Pro Met His Tyr Lys Leu 530 535 540 GCG ATA TTC CTG CAT ACC CTT GAT CTA TTG Απ GCC CGA GGC GAC AGC 1680

Ala He Phe Leu His Thr Leu Asp Leu Leu He Ala Arg Gly Asp Ser 545 550 555 560 238- This paper size applies to China National Standard (CNS) Α4 size (210 × 297 mm) Please read the precautions before you write Printed by the Consumer Standards Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs on this page

Ala Tyr Arg Gin Leu Glu Arg Asp Thr Leu Val Glu Ala Lys Met Tyr 565 570 575 TAC ATT CAG GCA CM CAG CTA CTG GGA COG CGC CCT GAT ATC CAT ACC 776

Tyr He Gin Ala Gin Gin Leu Leu Gly Pro Arg Pro Asplie His Thr 580 585 590 ACC MT ACT TGG CCA MT CCC ACC TTG AGT AAA GM GCT GGC GCT ATT 1824

Thr Asn Thr Trp Pro Asn Pro Thr Leu Ser Lys Glu Ala Gly Ala lie 595 600 605 GCC ACA COG ACA TTC CTC AGT TCA COG GAG GTG ATG AOG ITC GCT GCC 1872

Ala Thr Pro Thr Phe Leu Ser Ser Pro Glu Val Met Thr Phe Ala Ala 610 615 620 TGG CTA AGC 1881

Trp Leu Ser 625 ⑵Sequence ID No. 28: (i) Sequence characteristics: (A) Length: 627 amino acids (B) Type: Amino acid (D) Form: Linear (ii) Molecular type: Protein (xi) sequence description: sequence identification number 28 (TcaBi protein):

Met Ser Glu Ser Leu Phe Thr Gin Thr Leu Lys Glu Ala Arg Arg Asp 15 10 15 Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs

Ala Leu Val Ala His Tyr He Ala Thr Gin Val Pro Ala Asp Leu Lys 20 25 30

Glu Ser He Gin Thr Ala Asp Asp Leu Tyr Glu Tyr Leu Leu Leu Asp 35 40 45

Thr Lys He Ser Asp Leu Val Thr Thr Ser Pro Leu Ser Glu Ala He 50 55 60 039 ^ — This paper size applies to Chinese National Standard (CNS) A4 specification (210X297 mm) 509722 A7 ____B7 V. Description of the invention (237)

Gly Ser Leu Gin Leu Phe lie His Arg Ala lie Glu Gly Tyr Asp Gly 65 70 75 80

Thr Leu Ala Asp Ser Ala Lys Pro Tyr Phe Ala Asp Glu Gin Phe Leu 85 90 95

Tyr Asn Trp Asp Ser Phe Asn His Arg Tyr Ser Thr Trp Ala Gly Lys 100 105 110

Glu Arg Leu Lys Phe Tyr Ala Gly Asp Tyr He Asp Pro Thr Leu Arg 115 120 125

Leu Asn Lys Thr Glu lie Phe Thr Ala Phe Glu Gin Gly He Ser Gin 130 135 140

Gly Lys Leu Lys Ser Glu Leu Val Glu Ser Lys Leu Arg Asp Tyr Leu 145 150 155 160

He Ser Tyr Asp Thr Leu Ala Thr Leu Asp Tyr He Thr Ala Cys Gin 165 170 175

Gly Lys Asp Asn Lys Thr He Phe Phe lie Gly Arg Thr Gin Asn Ala 180 185 190

Pro Tyr Ala Phe Tyr Trp Arg Lys Leu Thr Leu Val Thr Asp Gly Gly 195 200 205

Lys Leu Lys Pro Asp Gin Trp Ser Glu Trp Arg Ala He Asn Ala Gly 210 215 220 lie Ser Glu Ala Tyr Ser Gly His Val Glu Pro Phe Trp Glu Asn Asn 225 230 235 240 Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs

Lys Leu His lie Arg Trp Phe Thr He Ser Lys Glu Asp Lys He Asp 245 250 255

Phe Val Tyr Lys Asn He Trp Val Met Ser Ser Asp Tyr Ser Trp Ala 260 265 270

Ser Lys Lys Lys Hes Leu Glu Leu Ser Phe Thr Asp Tyr Asn Arg Val 275 280 285 ____. 240- This paper size applies to China National Standard (CNS) A4 (210X 297 mm) 509722 A7 B7 V. Description of the invention (238 )

Gly Ala Thr Gly Ser Ser Ser Pro Thr Glu Val Ala Ser Gin Tyr Gly 290 295 300

Ser Asp Ala Gin Met Asn lie Ser Asp Asp Gly Thr Val Leu lie Phe 305 310 315 320

Gin Asn Ala Gly Gly Ala Thr Pro Ser Thr Gly Val Thr Leu Cys Tyr 325 330 335

Asp Ser Gly Asn Val He Lys Asn Leu Ser Ser Thr Gly Ser Ala Asn 340 345 350

Leu Ser Ser Lys Asp Tyr Ala Thr Thr Lys Leu Arg Met Cys His Gly 355 360 365

Gin Ser Tyr Asn Asp Asn Asn Tyr Cys Asn Phe Thr Leu Ser lie Asn 370 375 380

Thr lie Glu Phe Thr Ser Tyr Gly Thr Phe Ser Ser Asp Gly Lys Gin 385 390 395 400

Phe Thr Pro Pro Ser Gly Ser Ala He Asp Leu His Leu Pro Asn Tyr 405 410 415

Val Asp Leu Asn Ala Leu Leu Asp He Ser Leu Asp Ser Leu Leu Asn 420 425 430

Tyr Asp Val Gin Gly Gin Phe Gly Gly Ser Asn Pro Val Asp Asn Phe 435 440 445

Ser Gly Pro Tyr Gly He Tyr Leu Trp Glu lie Phe Phe His He Pro 450 455 460 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the precautions on the back before filling this page)

Phe Leu Val Thr Val Arg Met Gin Thr Glu Gin Arg Tyr Glu Asp Ala 465 470 475 480

Asp Thr Trp Tyr Lys Tyr lie Phe Arg Ser Ala Gly Tyr Arg Asp Ala 485 490 495

Asn Gly Gin Leu He Met Asp Gly Ser Lys Pro Arg Tyr Trp Asn Val 500 505 510 -------- J2AL · — This paper size applies to China National Standard (CNS) A4 (210X 297 mm) 509722 Printed by the Consumers' Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs A7 B7 V. Invention Description

Met Pro Leu Gin Leu Asp Thr Ala Trp Asp Thr Thr Gin Pro Ala Thr 515 520 525

Thr Asp Pro Asp Val lie Ala Met Ala Asp Pro Met His Tyr Lys Leu 530 535 540

Ala lie Phe Leu His Thr Leu Asp Leu Leu He Ala Arg Gly Asp Ser 545 550 555 560

Ala Tyr Arg Gin Leu Glu Arg Asp Thr Leu Val Glu Ala Lys Met Tyr 565 570 575

Tyr lie Gin Ala Gin Gin Leu Leu Gly Pro Arg Pro Asp He His Thr 580 585 590

Thr Asn Thr Trp Pro Asn Pro Thr Leu Ser Lys Glu Ala Gly Ala lie 595 600 605

Ala Thr Pro Thr Phe Leu Ser Ser Pro Glu Val Met Thr Phe Ala Ala 610 615 620

Trp Leu Ser 625 (2) Information of sequence identification number 29: (i) Sequence characteristics: (A) Length: 1689 base pairs (B) Type · Nucleic acid (C) Number of strands: double strand (D) Morphology: linear (ii) Molecular type: DNA (genome) (ix) Characteristics:

(A) Name / Tip: CDS (B) Location: 1..1689 (D) Other information: tcaBii (xi) Sequence description: Sequence identification number 29 (tcaBi i coding area): GCA GGC GAT ACC GCA MT Απ GGC GAC GGT GAT TTC TTG OCA CCG TAC 48

Ala Gly Asp Thr Ala Asn lie Gly Asp Gly Asp Phe Leu Pro Pro Tyr 1 5 10 15 This paper size applies to China National Standard (CNS) A4 size (210X 297 mm) (Please read the precautions on the back before filling this page )-· &Quot; Γ 509722 A7 B7 V. Description of invention (240) AAC GAT GTA CTA CTC GGT TAC TGG GAT AAA CIT GAG m CGC CTA TAC 96 Asn Asp Val Leu Leu Gly Tyr Trp Asp Lys Leu Glu Leu Arg Leu Tyr 20 25 30 AAC CTG OGC CAC AAT CTG AGT CTG GAT ΟΠ1 CM COG CTA AAT CTG OCA Asn Leu Arg His Asn Leu Ser Leu Asp Gly Gin Pro Leu Asn Leu Pro 35 40 45 144 CTG TAT GCC ACG CCG GTA GAC CCG AAA ACC CKJ CM CGC CAG CM GCC 192 Leu Tyr Ala Thr Pro Val Asp Pro Lys Thr Leu Gin Arg Gin Gin Ala 50 55 60 GGA GGG GAC GGT ACA GGC AGT AGT CCG GCT GGT GGT CM GGC AGT GTT 240 Gly Gly Asp Gly Thr Gly Ser Ser Pro Ala Gly Gly Gin Gly Ser Val 65 70 75 80 CAG GGC TGG CGC TAT CCG m TTG GTA GM CGC GCC CGC TGC GCT GTG 288 Gin Gly Trp Arg Tyr Pro Leu Leu Val Glu Arg Ala Arg Ser Ala Val 85 90 95 AGT TTG TTG ACT CAG TO GGC AAC AGC m CM ACA A CG m GAA CAT 336 Ser Leu Leu Thr Gin Phe Gly Asn Ser Leu Gin Thr Thr Leu Glu His 100 105 110 CAG GAT AAT GAA AAA ATG ACG ATA CTG TTG CAG ACT CAA CAG GAA GCC 384 Gin Asp Asn Glu Lys Met Thr He Leu Leu Gin Thr Gin Gin Glu Ala 115 120 125

Printed by ATC CTG AAA CAT CAG CAC GAT ATA CAA CAA AAT AAT CTA AAA GGA m lie Leu Lys His Gin His Asp He Gin Gin Asn Asn Leu Lys Gly Leu 130 135 140 432 CAA CAC AGC CTG ACC GCA m CAG GCT AGC CGT GAT GGC GAC ACA TTG 480 Gin His Ser Leu Thr Ala Leu Gin Ala Ser Arg Asp Gly Asp Thr Leu 145 150 155 160 CGG CM AM CAT TAC AGC GAC CTG Απ MC GGT GGT CTA TCT GCG GCA 528 Arg Gin Lys His Tyr Ser Asp Leu lie Asn Gly Gly Leu Ser Ala Ala 165 170 175 This paper size applies to the Chinese National Standard (CNS) A4 specifications (^ centimeters) Printed by the Central Consumers Bureau of the Ministry of Economic Affairs Employee Cooperatives 509722 A7 B7 5 Description of the invention (241) GAA ATC GCC GGT CTG ACA CTA GGC AGC ACC GCC ATG ΑΉ ACT AAT GGC 576

Glu He Ala Gly Leu Thr Leu Arg Ser Thr Ala Met lie Thr Asn Gly 180 185 190 GTT GCA ACG GGA TTG CTG ATT GCC GGC GGA ATC GCC MC GOG GTA CCT 624

Val Ala Thr Gly Leu Leu lie Ala Gly Gly He Ala Asn Ala Val Pro 195 200 205 MC GTC TTC GGG CTG GCT MC GGT GGA TCG GM TGG GGA GCG CCA m 672

Asn Val Phe Gly Leu Ala Asn Gly Gly Ser Glu Trp Gly Ala Pro Leu 210 215 220 ATT GGC TGC GGC CM GCA AGC CM GTT GGC GCC GGC ATC CAG GAT CAG 720

He Gly Ser Gly Gin Ala Thr Gin Val Gly Ala Gly He Gin Asp Gin 225 230 235 240 AGC GCG GGC ATT TCA GAA GTG ACA GCA GGC TAT CAG CGT CGT CAG GAA 768

Ser Ala Gly lie Ser Glu Val Thr Ala Gly Tyr Gin Arg Arg Gin Glu 245 250 255 GAA TGG GCA TKJ CM CGG GAT ATT GCT GAT AAC GAA ATA ACC CM CTG 816

Glu Trp Ala Leu Gin Arg Asp He Ala Asp Asn Glu He Thr Gin Leu 260 265 270 GAT GCC CAG ATA CM AGC CTG CM GAG CM ATC AOG ΑΊΌ GCA CM AAA 864

Asp Ala Gin lie Gin Ser Leu Gin Glu Gin lie Thr Met Ala Gin Lys 275 280 285 CAG ATC ACXj CTC TCT GAA AGC GAA CAA GOG AAT GGC CAA GCG Απ TAT 912

Gin He Thr Leu Ser Glu Thr Glu Gin Ala Asn Ala Gin Ala He Tyr 290 295 300 GAC CTG CM ACC ACT CGT ΤΓΤ ACC GGG CAG GCA CTG TAT MC TGG ATG 960

Asp Leu Gin Thr Thr Arg Phe Thr Gly Gin Ala Leu Tyr Asn Trp Met 305 310 315 320 GCC GGT CGT CTC TCC GCG CTC TAT TAC CM ATG TAT GAT TCC ACT CTG 1008

Ala Gly Arg Leu Ser Ala Leu Tyr Tyr Gin Met Tyr Asp Ser Thr Leu 325 330 335 CCA ATC TGT CTC CAG CCA AAA GCC GCA m GTA CAG GAA m GGC GAG 1056

Pro He Cys Leu Gin Pro Lys Ala Ala Leu Val Gin Glu Leu Gly Glu-^ 244-— This paper size applies to the Chinese National Standard (CNS) A4 specification (210 X 297 mm) (Please read the precautions on the back before filling (This page)

509722 A7 B7 V. Description of the invention (242) 340 345 350 Printed by AAA GAG AGC GAC AGT OT TTC CAG (ΠΤ CCG GTC TGG AAT GAT CTG TGG 1104 Lys Glu Ser Asp Ser Leu Phe Gin Val Pro Val Trp Asn Asp Leu Trp 355 360 365 CM GGG CTG ΤΓΑ GCA GGA GAA GGT m AGT TCA GAG CTA CAG AAA CTG 1152 Gin Gly Leu Leu Ala Gly Glu Gly Leu Ser Ser Glu Leu Gin Lys Leu 370 375 380 GAT GCC ATC TGG CTT GCA CGT GGT GGT Απ GGG CTA GM GCC ATC OGC 1200 Asp Ala lie Trp Leu Ala Arg Gly Gly lie Gly Leu Glu Ala He Arg 385 390 395 400 ACC GTG TCG CTG GAT ACC CTG ΉΤ GGC ACA GGG AOG m AGT GM MT Thr Val Ser Leu Asp Thr Leu Phe Gly Thr Gly Thr Leu Ser Glu Asn 405 410 415 ATC AAT AAA GTG CTT AAC GGG GM AOG GTA TCT CCA TCC GGT GGC GTC 1296 lie Asn Lys Val Leu Asn Gly Glu Thr Val Ser Pro Ser Gly Gly Val 420 425 430 ACT CTG GCG CTG ACA GGG GAT ATC m CAA GCA ACA CTG GAT TTG AGT 1344 Thr Leu Ala Leu Thr Gly Asp He Phe Gin Ala Thr Leu Asp Leu Ser 435 440 445 CAG CTA GGTTTG GAT AAC TCT TA C AAC TTG GGT AAC GAG AAG AAA CX5T 1392 Gin Leu Gly Leu Asp Asn Ser Tyr Asn Leu Gly Asn Glu Lys Lys Arg 450 455 460 CGT Απ AAA CGT ATC GCC GTC ACC CTO (XA ACA CIT CTG GGG CTA TAT 1440 Arg He Ly Arg He Ala Val Thr Leu Pro Thr Leu Leu Gly Pro Tyr 465 470 475 480 CM GAT CTT GAA GCC ACA CTG GTA ATG GGT GCG GM ATC GCC GCC ΤΓΑ 1488 Gin Asp Leu Glu Ala Thr Leu Val Met Gly Ala Glu He Ala Ala Leu 485 490 495 TCA CAC GGT GTG MT GAC GGA GGC CGG ΤΤΓ GTT ACC GAC ΉΤ AAC GAC 1536 Ser His Gly Val Asn Asp Gly Gly Arg Phe Val Thr Asp Phe Asn Asp 500 505 510 245- This paper standard applies to Chinese national standards (CNS ) A4 specification (210X297 mm) Please read the notes on the back i Printed by the Gathering Line Printed by the Consumers' Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs 509722 A7 B7 5. Invention Description (243) AGC CGT CCT CTG CCT ΤΤ GM GGT CGA GAT GCA ACA ACC GGC ACA CTG 1584

Ser Arg Phe Leu Pro Phe Glu Gly Arg Asp Ala Thr Thr Gly Thr Leu 515 520 525 GAG CTC AAT ATT TO CAT GCG GGT AAA GAG GGA ACG CAA CAC GAG TTG 1632

Glu Leu Asn He Phe His Ala Gly Lys Glu Gly Thr Gin His Glu Leu 530 535 540 GTC GCG AAT CTG AGT GAC ATC Απ GTG CAT CTG AAT TAC ATC Απ CGA 1680

Val Ala Asn Leu Ser Asp He lie Val His Leu Asn Tyr He lie Arg 545 550 555 560 GAC GCG TM 1689

Asp Ala * 资料 Sequence ID No. 30: (i) Sequence characteristics: (A) Length: 562 amino acids (B) Type: Amino acid (D) Form: Linear (ii) Molecular type: Protein (xi) Sequence description: Sequence identification number 30 (TcaBii protein):

Ala Gly Asp Thr Ala Asn He Gly Asp Gly Asp Phe Leu Pro Pro Tyr 15 10 15

Asn Asp Val Leu Leu Gly Tyr Trp Asp Lys Leu Glu Leu Arg Leu Tyr 20 25 30

Asn Leu Arg His Asn Leu Ser Leu Asp Gly Gin Pro Leu Asn Leu Pro 35 40 45

Leu Tyr Ala Thr Pro Val Asp Pro Lys Thr Leu Gin Arg Gin Gin Ala 50 55 60

Gly Gly Asp Gly Thr Gly Ser Ser Pro Ala Gly Gly Gin Gly Ser Val 65 70 75 80

Gin Gly Trp Arg Tyr Pro Leu Leu Val Glu Arg Ala Arg Ser Ala Val 85 90 95

Ser Leu Leu Thr Gin Phe Gly Asn Ser Leu Gin Phe Gly Asn Ser Leu Gin Thr Thr Leu Glu His ____ J44 ---- This paper size applies to China National Standard (CNS) A4 specification (210X297 mm) (Please read the precautions on the back before filling this page ) • I · Order · Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 509722 A7 B7 V. Description of Invention (244) 100 105 110

Gin Asp Asn Glu Lys Met Thr lie Leu Leu Gin Thr Gin Gin Glu Ala 115 120 125 lie Leu Lys His Gin His Asp He Gin Gin Asn Asn Leu Lys Gly Leu 130 135 140

Gin His Ser Leu Thr Ala Leu Gin Ala Ser Arg Asp Gly Asp Thr Leu 145 150 155 160

Arg Gin Lys His Tyr Ser Asp Leu lie Asn Gly Gly Leu Ser Ala Ala 165 170 175

Glu lie Ala Gly Leu Thr Leu Arg Ser Thr Ala Met He Thr Asn Gly 180 185 190

Val Ala Thr Gly Leu Leu lie Ala Gly Gly lie Ala Asn Ala Val Pro 195 200 205

Asn Val Phe Gly Leu Ala Asn Gly Gly Ser Glu Trp Gly Ala Pro Leu 210 215 220

He Gly Ser Gly Gin Ala Thr Gin Val Gly Ala Gly lie Gin Asp Gin 225 230 235 240

Ser Ala Gly He Ser Glu Val Thr Ala Gly Tyr Gin Arg Arg Gin Glu 245 250 255

Glu Trp Ala Leu Gin Arg Asp He Ala Asp Asn Glu lie Thr Gin Leu 260 265 270

Asp Ala Gin He Gin Ser Leu Gin Glu Gin lie Thr Met Ala Gin Lys 275 280 285

Gin He Thr Leu Ser Glu Thr Glu Gin Ala Asn Ala Gin Ala He Tyr 290 295 300

Asp Leu Gin Thr Thr Arg Phe Thr Gly Gin Ala Leu Tyr Asn Trp Met 305 310 315 320

Ala Gly Arg Leu Ser Ala Leu Tyr Tyr Gin Met Tyr Asp Ser Thr Leu -— This paper size applies to China National Standard (CNS) A4 specification (210X297 mm) (Please read the precautions on the back before filling this page) Order 509722 A7 B7 V. Description of the invention (245) 325 330 335

Pro He Cys Leu Gin Pro Lys Ala Ala Leu Val Gin Glu Leu Gly Glu 340 345 350

Lys Glu Ser Asp Ser Leu Phe Gin Val Pro Val Trp Asn Asp Leu Trp 355 360 365

Gin Gly Leu Leu Ala Gly Glu Gly Leu Ser Ser Glu Leu Gin Lys Leu 370 375 380

Asp Ala lie Trp Leu Ala Arg Gly Gly lie Gly Leu Glu Ala lie Arg 385 390 395 400

Thr Val Ser Leu Asp Thr Leu Phe Gly Thr Gly Thr Leu Ser Glu Asn 405 410 415 lie Asn Lys Val Leu Asn Gly Glu Thr Val Ser Pro Ser Gly Gly Val 420 425 430

Thr Leu Ala Leu Thr Gly Asp lie Phe Gin Ala Thr Leu Asp Leu Ser 435 440 445

Gin Leu Gly Leu Asp Asn Ser Tyr Asn Leu Gly Asn Glu Lys Lys Arg 450 455 460

Arg He Lys Arg lie Ala Val Thr Leu Pro Thr Leu Leu Gly Pro Tyr 465 470 475 480

Gin Asp Leu Glu Ala Thr Leu Val Met Gly Ala Glu He Ala Ala Leu 485 490 495 Printed by the Staff Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling out this page)

Ser His Gly Val Asn Asp Gly Gly Arg Phe Val Thr Asp Phe Asn Asp 500 505 510

Ser Arg Phe Leu Pro Phe Glu Gly Arg Asp Ala Thr Thr Gly Thr Leu 515 520 525

Glu Leu Asn He Phe His Ala Gly Lys Glu Gly Thr Gin His Glu Leu 530 535 540

Val Ala Asn Leu Ser Asp He lie Val His Leu Asn Tyr He lie Arg ___- ⑽- This paper size applies to China National Standard (CNS) A4 (210X 297 mm) Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs 509722 A7 ____ B7 V. Description of the invention (246) 545 550 555 560

Asp Ala * (2) Information of sequence identification number 31: (i) Sequence characteristics: (A) Length: 4458 base pairs (B) Type: Nucleic acid (C) Number of strands: Double strand (D) Form: Linear (ii) Molecular type: DNA (genome) (A & name / tip: CDS (B) position: 1 .. 4458 (xi) sequence description: sequence identification number 31 (tcac gene): ATG CAG GAT TCA CCA GAA GTA T0G Απ ACA AOG CTC TCA CTT CCC AAA 48

Met Gin Asp Ser Pro Glu Val Ser He Thr Thr Leu Ser Leu Pro Lys 15 10 15 GGT GGC GGT GCT ATC MT GGC ATG GGA GM GCA CTG MT GCT GCC GGC 96

Gly Gly Gly Ala lie Asn Gly Met Gly Glu Ala Leu Asn Ala Ala Gly 20 25 30 CIT GAT GGA ATG GO: TCC CTA TCT CTG CCA m CCC CIT TCG ACC GGC 144

Pro Asp Gly Met Ala Ser Leu Ser Leu Pro Leu Pro Leu Ser Thr Gly 35 40 45 AGA GGG ACXj GCT CCT GGA m TCG CTG ATT TAC AGC AAC AGT GCA GGT 192

Arg Gly Thr Ala Pro Gly Leu Ser Leu He Tyr Ser Asn Ser Ala Gly 50 55 60 MT GGG CCT TTC GGC ATC GGC TGG CM TCC GGT GTT ATC TCC ATT AGC 240

Asn Gly Pro Phe Gly lie Gly Trp Gin Cys Gly Val Met Ser lie Ser 65 70 75 80 OGA CGC ACC CM CAT GGC Απ CCA CM TAC GGT MT GAC GAC ACG TTC 288

Arg Arg Thr Gin His Gly He Pro Gin Tyr Gly Asn Asp Asp Thr Phe 85 90 95 CTA TCC CCA CM GGC GAG GTC ATG AAT ATC GCC CTG AAT GAC CM GGG 336

Leu Ser Pro Gin Gly Glu Val Met Asn He Ala Leu Asn Asp Gin Gly 100 105 110 549_ This paper size applies to China National Standard (CNS) A4 (210X 297 mm) (Please read the precautions on the back before filling this page )

509722 A 7 B7 V. Description of the Invention (247) CM CCT GAT ATC CGT CM GAC GTT AAA ACG CTG CM GGC GTT ACC ITG 384

Gin Pro Asp lie Arg Gin Asp Val Lys Thr Leu Gin Gly Val Thr Leu 115 120 125 CCA Απ TCC TAT ACC GTG ACC CGC TAT CAA GCC GGC CAG ATC CTG GAT 432

Pro lie Ser Tyr Thr Val Thr Arg Tyr Gin Ala Arg Gin lie Leu Asp 130 135 140 TTC ACT MA ATC GM TAC TGG CM CCT GCC TCC GGT CM GM GGA CGC 480

Phe Ser Lys He Glu Tyr Trp Gin Pro Ala Ser Gly Gin Glu Gly Arg 145 150 155 160 (ΓΓ TO TGG CTG ATA TGG ACA COG GAC GGG CAT CTA CAC ATC ΤΓΑ GGG 528

Ala Phe Trp Leu lie Ser Thr Pro Asp Gly His Leu His He Leu Gly 165 170 175 AAA ACC GCG CAG GCT TGT CTG GCA MT COG CM AAT GAC CM CM ATC 576

Lys Thr Ala Gin Ala Cys Leu Ala Asn Pro Gin Asn Asp Gin Gin lie 180 185 190 GCC CAG TCG TTG CTG GAA GAA ACT (ΠΌ ACG CCA GCT GGT GAA CAT GTC 624

Ala Gin Trp Leu Leu Glu Glu Thr Val Thr Pro Ala Gly Glu His Val 195 200 205 AGC TAT CM TAT OGA GCC GAA GAT GAA GCC CAT TGT GAC GAC AAT GAA 672

Ser Tyr Gin Tyr Arg Ala Glu Asp Glu Ala His Cys Asp Asp Asn Glu 210 215 220 AAA ACC GCT CAT (XC AAT GTT ACC GCA CAG CGC TAT CTG GTA CAG GTG 720

Lys Thr Ala His Pro Asn Val Thr Ala Gin Arg Tyr Leu Val Gin Val 225 230 235 240 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling this page) AAC TAC GGC MC ATC AAA CCA CAA GCC AGC CTG TTC GTA CTG GAT MC 768

Asn Tyr Gly Asn He Lys Pro Gin Ala Ser Leu Phe Val Leu Asp Asn 245 250 255 GCA CCT CCC GCC CCG GM GAG TGG CTG ΉΤ CAT CTG GTC ΉΤ GAC CAC 816

Ala Pro Pro Ala Pro Glu Glu Trp Leu Phe His Leu Val Phe Asp His 260 265 270 GGT GAG CGC GAT ACT TCA CTT CAT ACC GTG CCA ACA TGG GAT GCA GGT 864 This paper size applies the Chinese National Standard (CNS) A4 specification (centimeter) ) 509722 A7 B7 V. Description of the invention (2you)

Gly Glu Arg Asp Thr Ser Leu His Thr Val Pro Thr Trp Asp Ala Gly 275 280 285 ACA GCG CAA TGG TCT GTA CGC CCG GAT ATC TTC TCT CGC TAT GAA TAT 912

Thr Ala Gin Trp Ser Val Arg Pro Asp He Phe Ser Arg Tyr Glu Tyr 290 295 300 GGT TIT GAA GTG CGT ACT CGC CGC πΑ TGT CAA CM GTG CTG ATG ΉΤ 960

Gly Phe Glu Val Arg Thr Arg Arg Leu Cys Gin Gin Val Leu Met Phe 305 310 315 320 CAC CGC ACC GCG CTC ATG GO: GGA GAA GCX: AGT ACC AAT GAC GCC CCG 1008

His Arg Thr Ala Leu Met Ala Gly Glu Ala Ser Thr Asn Asp Ala Pro 325 330 335 GAA CTG GTT GGA CGC m ATA CTG GAA TAT GAC AAA AAC G (X AGC GTC 1056

Glu Leu Val Gly Arg Leu He Leu Glu Tyr Asp Lys Asn Ala Ser Val 340 345 350 ACT ACG TTG ATT ACC ATC 03T CAA m AC3C CAT GAA TOG GAC GGG AGG 1104

Thr Thr Leu He Thr lie Arg Gin Leu Ser His Glu Ser Asp Gly Arg 355 360 365 CCA GTC ACC CAG CCA CCA CTA GAA CTA GCX: TGG CAA CGG ΉΤ GAT CTG 1152

Pro Val Thr Gin Pro Pro Leu Glu Leu Ala Trp Gin Arg Phe Asp Leu 370 375 380 GAG AAA ATC CCG ACA TGG CAA CGC ΤΓΤ GAC GCA CTA GAT AAT TIT AAC 1200

Glu Lys He Pro Thr Trp Gin Arg Phe Asp Ala Leu Asp Asn Phe Asn 385 390 395 400 TCG CAG CM CGT TAT CM CTG GTT GAT CTG CGG GGA GAA GGG TTG CCA 1248 Read the notes on the back and fill out this page) 丨 Min ·

Ser Gin Gin Arg Tyr Gin Leu Val Asp Leu Arg Gly Glu Gly Leu Pro 405 410 415 GGT ATG CTG TAT CAA GAT CGA GGC GCT TGG TGG TAT AAA GCT COG CM 1296

Gly Met Leu Tyr Gin Asp Arg Gly Ala Trp Trp Tyr Lys Ala Pro Gin 420 425 430 ΟίΤ CAG GAA GAC GGA GAC AGC AAT GCC GTC ACT TAC GAC AAA ATC GCC 1344

Arg Gin Glu Asp Gly Asp Ser Asn Ala Val Thr Tyr Asp Lyslie Ala 435 440 445 ---_ ^ 25L · — This paper size applies to China National Standard (CNS) A4 (210X 297 mm) Central Standards Bureau of the Ministry of Economic Affairs Printed by Employee Consumer Cooperatives 509722 A7 B7 V. Description of Invention (249) CCA CTG CCT ACC CTA (XC AAT TTG CAG GAT AAT GCC TCA TTG ATG GAT 1392

Pro Leu Pro Thr Leu Pro Asn Leu Gin Asp Asn Ala Ser Leu Met Asp 450 455 460 ATC MC GGA GAC GGC CM CTG GAT TGG GTT GIT ACC GCC TCC GGT ATT 1440

He Asn Gly Asp Gly Gin Leu Asp Trp Val Val Thr Ala Ser Gly lie 465 470 475 480 CX3C GGA TAC CAT ACT CAG CAA CCC GAT GGA AAG TGG ACG CAC TIT ACG 1488

Arg Gly Tyr His Ser Gin Gin Pro Asp Gly Lys Trp Thr His Phe Thr 485 490 495 CCA ATC MT GCC TTG CCC GTG GAA TAT ΤΓΤ CAT CCA AGC ATC CAG TTC 1536

Pro He Asn Ala Leu Pro Val Glu Tyr Phe His Pro Ser lie Gin Phe 500 505 510 GCT GAC CTT ACC GGG GCA GGC m TCT GAT m GTG TTG ATC GGG CCG 1584

Ala Asp Leu Thr Gly Ala Gly Leu Ser Asp Leu Val Leu He Gly Pro 515 520 525 AM AGC GTG CXJT CTA TAT GCC AAC CAG CGA MC GGC TXXJ CGT AAA GGA 1632

Lys Ser Val Arg Leu Tyr Ala Asn Gin Arg Asn Gly Trp Arg Lys Gly 530 535 540 GAA GAT GTC CCC CAA TCC ACA GCT ATC A (T CTG CCT GTC ACA GGG ACC 1680

Glu Asp Val Pro Gin Ser Thr Gly He Thr Leu Pro Val Thr Gly Thr 545 550 555 560 GAT GCC CGC AAA CTG GTG GCT TTC AGT GAT ATG CTC GGT TCC GGT CM 1728

Asp Ala Arg Lys Leu Val Ala Phe Ser Asp Met Leu Gly Ser Gly Gin 565 570 575 CAA CAT CTG GTG GM ATC MG GGT MT CGC GTC ACC TGT TGG CCG MT 1776

Gin His Leu Val Glu lie Lys Gly Asn Arg Val Thr Cys Trp Pro Asn 580 585 590 CTA GGG CAT GGC CGT TTC GGT CAA CCA CTA ACT CTG TCA GGA ΤΓΤ AGC 1824

Leu Gly His Gly Arg Phe Gly Gin Pro Leu Thr Leu Ser Gly Phe Ser 595 600 605 CAG CCC GM MT AGC TTC MT CCC GM CGG CTG TTT CTG GOG GAT ATC 1872 This paper size applies the Chinese National Standard (CNS) A4 specification (210X 297 mm) (Please read the notes on the back before filling out this page) Order * k · 509722 A7 B7 V. Description of the invention (25)

Gin Pro Glu Asn Ser Phe Asn Pro Glu Arg Leu Phe Leu Ala Asplie 610 615 620 GAC GGC TCC GGC ACC ACC GAC CTT ATC TAT (ΠΪ CM TXT GGC TCT TTG 1920

Asp Gly Ser Gly Thr Thr Asp Leu He Tyr Ala Gin Ser Gly Ser Leu 625 630 635 640 CTC Απ TAT CTC AAC CAA ACJT GGT AAT CAG ΤΓΤ GAT GCC CCG TTG ACA 1968

Leu lie Tyr Leu Asn Gin Ser Gly Asn Gin Phe Asp Ala Pro Leu Thr 645 650 655 m GCXj TTG CCA GAA C3GC GTA CAA ΤΤ GAC AAC ACT TGC CAA CTT CAA 2016

Leu Ala Leu Pro Glu Gly Val Gin Phe Asp Asn Thr Cys Gin Leu Gin 660 665 670 GTC GCC GAT ATT CAG GGA m GGG ATA GCC AGC TTG Απ CTG ACT GTG 2064

Val Ala Asp He Gin Gly Leu Gly He Ala Ser Leu He Leu Thr Val 675 680 685 CCA CAT ATC GOj CCA CAT CAC TGG CGT TGT GAC CTG TCA CTG ACC AAA 2112

Pro His lie Ala Pro His His Trp Arg Cys Asp Leu Ser Leu Thr Lys 690 695 700 CCC TGG TTG TTG MT GTA ATG MC AAT AAC CGG GGC GCA CAT CAC A0G 2160

Pro Trp Leu Leu Asn Val Met Asn Asn Asn Arg Gly Ala His His Thr 705 710 715 720 CTA CAT TAT CGT AGT TCC GCG CM TTC TGG TTG GAT GM AAA ΊΤΑ CAG 2208

Leu His Tyr Arg Ser Ser Ala Gin Phe Trp Leu Asp Glu Lys Leu Gin 725 730 735 CTC ACC MA GCA GGC MA TCT CCG GCT TGT TAT CTG CCG ΤΓΤ CCA ATG 2256 (Notes on the back then fill out this page)

Leu Thr Lys Ala Gly Lys Ser Pro Ala Cys Tyr Leu Pro Phe Pro Met 740 745 750 CAT TTG CTA TGG TAT ACC GAA ATT CAG GAT GAA ATC AGC GGC AAC CGG 2304

His Leu Leu Trp Tyr Thr Glu lie Gin Asp Glu He Ser Gly Asn Arg 755 760 765 CTC ACC AGT GAA GTC AAC TAC AGC CAC GGC GTC TGG GAT GGT AAA GAG 2352

Leu Thr Ser Glu Val Asn Tyr Ser His Gly Val Trp Asp Gly Lys Glu 770 775 780 ----- This paper size applies to China National Standard (CNS) A4 (210X 297 mm) 509722 Employees of the Central Standards Bureau of the Ministry of Economic Affairs Printed by the cooperative A7 B7 V. Description of the invention (251) CGG GM TTC AGA GGA TIT GGC TGC ATC AM CAG ACA GAT ACC ACA ACG 2400

Arg Glu Phe Arg Gly Phe Gly Cys lie Lys Gin Thr Asp Thr Thr Thr 785 790 795 800 ΤΤΓ TCT CAC GGC ACC GCC CCC GM CAG GCG GCA CCG TCG CTG AGT ATT 2448

Phe Ser His Gly Thr Ala Pro Glu Gin Ala Ala Pro Ser Leu Ser lie 805 810 815 ACJC TGG TTT GCC ACC GGC ATG GAT GAA GTA GAC AGC CM TTA GCT ACG 2496

Ser Trp Phe Ala Thr Gly Met Asp Glu Val Asp Ser Gin Leu Ala Thr 820 825 830 GAA TAT TGG CAG GCA GAC ACG CAA GCT TAT AGC GGA TTT GAA ACC CGT 2544

Glu Tyr Trp Gin Ala Asp Thr Gin Ala Tyr Ser Gly Phe Glu Thr Arg 835 840 845 TAT ACC GTC TGG GAT CAC ACC AAC CAG ACA GAC CAA GCA TIT ACC OCX: 2592

Tyr Thr Val Trp Asp His Thr Asn Gin Thr Asp Gin Ala Phe Thr Pro 850 855 860 MT GAG ACA CM CGT AAC TGG CTG ACG CGA GCG CTT AAA GGC CM CTG 2640

Asn Glu Thr Gin Arg Asn Trp Leu Thr Arg Ala Leu Lys Gly Gin Leu 865 870 875 880 CTA CGC ACT GAG CTC TAC GGT CTG GAC GGA ACA GAT AAG CAA ACA GTG 2688

Leu Arg Thr Glu Leu Tyr Gly Leu Asp Gly Thr Asp Lys Gin Thr Val 885 890 895 CCT TAT ACC GTC AGT GAA TCG CGC TAT CAG CTA CGC TCT ATT CCC GTA 2736

Pro Tyr Thr Val Ser Glu Ser Arg Tyr Gin Val Arg Ser He Pro Val 900 905 910 MT AAA GM ACT GM m TCT GCC TGG GTG ACT GCT Απ GM MT OGC 2784

Asn Lys Glu Thr Glu Leu Ser Ala Trp Val Thr Ala lie Glu Asn Arg 915 920 925 AGC TAC CAC TAT GAA CX3T ATC ATC ACT GAC CCA CAG TTC AGC CAG AGT 2832

Ser Tyr His Tyr Glu Arg He He Thr Asp Pro Gin Phe Ser Gin Ser 930 935 940 ATC AAG TTG CAA CAC GAT ATC ΊΤΓ GGT CAA TCA CTG CAA AGT GTC GAT 2880 This paper size applies to the Chinese National Standard (CNS) A4 specification (210X 297 mm) (Please read the notes on the back before filling this page)

509722 Printed by the Consumer Cooperatives of the Central Bureau of Standards of the Ministry of Economic Affairs A7 B7 V. Invention Description (252)

He Lys Leu Gin His Asp He Phe Gly Gin Ser Leu Gin Ser Val Asp 945 950 955 960 ATT GCC TGG CCG CGC CGC GM AAA CCA GCA GTG MT OCC TAC CCG CCT 2928 lie Ala Trp Pro Arg Arg Glu Lys Pro Ala Val Asn Pro Tyr Pro Pro 965 970 975 ACC CTG CCG GAA ACG CTA 7ΓΤ GAC AGC AGC TAT GAT GAT CM CAA CAA 2976

Thr Leu Pro Glu Thr Leu Phe Asp Ser Ser Tyr Asp Asp Gin Gin Gin 980 985 990 CTA m CGT CTG GTG AGA CM AAA MT AGC TGG CAT CAC CTG ACT GAT 3024

Leu Leu Arg Leu Val Arg Gin Lys Asn Ser Trp His His Leu Thr Asp 995 1000 1005 GGG GAA AAC TGG CGA ΤΓΑ GGT m ΟΧΪ AAT GCA CAA CX3C OGT GAT GTT 3072

Gly Glu Asn Trp Arg Leu Gly Leu Pro Asn Ala Gin Arg Arg Asp Val 1010 1015 1020 TAT ACT TAT GAC CGG AGC AAA Απ CCA ACC GAA GGG ATT TCC CTT GM 3120

Tyr Thr Tyr Asp Arg Ser Lys He Pro Thr Glu Gly lie Ser Leu Glu 1025 1030 1035 1040 ATC TTG CTG AAA GAT GAT GGC GTC CTG CTA GCA GAT GM AAA GCG GCC GTT 3168 lie Leu Leu Lys Asp Asp Gly Leu Leu Ala Asp Glu Lys Ala Ala Val 1045 1050 1055 TAT CTG GGA CAA CAA CAG ACG ΤΓΤ TAC ACC GCC GGT CAA GOG GAA GTC 3216

Tyr Leu Gly Gin Gin Gin Thr Phe Tyr Thr Ala Gly Gin Ala Glu Val 1060 1065 1070 ACT CTA GM AAA CCC AOG m CAA GCA CTG GTC GCG TTC CM GAA ACC 3264

Thr Leu Glu Lys Pro Thr Leu Gin Ala Leu Val Ala Phe Gin Glu Thr 1075 1080 1085 GCX: ATG ATG GAC GAT ACC TCA TTA CAG GCG TAT GAA GGC GTG ATT GAA 3312

Ala Met Met Asp Asp Thr Ser Leu Gin Ala Tyr Glu Gly Val lie Glu 1090 1095 1100 GAG CAA GAG TTG AAT ACC GCG CTG ACA CAG GCC GGT TAT CAG CAA GTC 3360

Glu Gin Glu Leu Asn Thr Ala Leu Thr Gin Ala Gly Tyr Gin Gin Val 1105 1110 1115 1120 -255. This paper size applies to the Chinese National Standard (CNS) A4 specification (210X 297 mm) (Please read the precautions on the back first) (Fill in this page)

509722 A7 __B7_ V. Description of the Invention (253) GCG CGG TTG ΉMT MT ACC AGA TCA GM AGC CCG GTA TGG GOG GCA CGG 3408

Ala Arg Leu Phe Asn Thr Arg Ser Glu Ser Pro Val Trp Ala Ala Arg 1125 1130 1135 CM GGT TAT ACC GAT TAC GGT GAC GGC GCA CAG TTC TGG CGG CCT CAG 3456

Gin Gly Tyr Thr Asp Tyr Gly Asp Ala Ala Gin Phe Trp Arg Pro Gin 1140 1145 1150 GCT CAG (OT MC TCG ΉΌ CTO ACA GGG AAA ACC ACA CTG ACC TGG GAT 3504

Ala Gin Arg Asn Ser Leu Leu Thr Gly Lys Thr Thr Leu Thr Trp Asp 1155 1160 1165 ACC CAT CAT TGT GTA ΑΑ ATA CAG ACT CM GAT GCC GCT GGA m ACG 3552

Thr His His Cys Val He lie Gin Thr Gin Asp Ala Ala Gly Leu Thr 1170 1175 1180 ACXJ CM GCC CAT TAC GAT TAT CGT TTC CIT ACA (TG GTA CM CTG ACA 3600

Thr Gin Ala His Tyr Asp Tyr Arg Phe Leu Thr Pro Val Gin Leu Thr 1185 1190 1195 1200 GAT ATT AAT GAT AAT CAA CAT Απ GTG ACT CTG GAC GOG CTA GGT CX5C 3648

Asp He Asn Asp Asn Gin His He Val Thr Leu Asp Ala Leu Gly Arg 1205 1210 1215 GTA ACC AOC AGC CGG TTC TGG GGC ACA GAG GCA GGA CAA GCC GCA GGC 3696

Val Thr Thr Ser Arg Phe Trp Gly Thr Glu Ala Gly Gin Ala Ala Gly 1220 1225 1230 TAT TGC AAC CAG CCC TTC ACA CCA CCG GAC TXT GTA GAT AAA GCG CTG 3744

Tyr Ser Asn Gin Pro Phe Thr Pro Pro Asp Ser Val Asp Lys Ala Leu 1235 1240 1245 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling this page) GCA m ACC GGC GCA CTC CCT OT GCC CM TGT m GTC TAT GCC GIT 3792

Ala Leu Thr Gly Ala Leu Pro Val Ala Gin Cys Leu Val Tyr Ala Val 1250 1255 1260 GAT AGC TGG ATG CCG TCG m TCTITG TCT CAG CIT TCT CAG TCA CAA 3840

Asp Ser Trp Met Pro Ser Leu Ser Leu Ser Gin Leu Ser Gin Ser Gin 1265 1270 1275 1280 GM GAG GCA GM GCG CTA TGG GCG CM CTG CGT GCC GCT CAT ATG ATT 3888 -256- This paper size applies to Chinese National Standard (CNS) A4 specifications (210X 297 mm) 509722 A7 _B7_ V. Description of the invention (254)

Glu Glu Ala Glu Ala Leu Trp Ala Gin Leu Arg Ala Ala His Met lie 1285 1290 1295 ACC GM GAT GGG MA GTG TGT GOG m AGC GGG MA CGA GGA ACA AGC 3936

Thr Glu Asp Gly Lys Val Cys Ala Leu Ser Gly Lys Arg Gly Thr Ser 1300 1305 1310 CAT CAG AAC CTG ACG Απ CAA CTT ΑΠ TCG CTA TTG GCA AGT Απ OCX: 3984

His Gin Asn Leu Thr lie Gin Leu He Ser Leu Leu Ala Ser He Pro 1315 1320 1325 OJT ΊΤΑ CXXj CCA CAT GTA CTG GGG ATC ACT ACT GAT CGC TAT GAT AGC 4032

Arg Leu Pro Pro His Val Leu Gly He Thr Thr Asp Arg Tyr Asp Ser 1330 1335 1340 GAT CXXj CAA CAG CAG CAC CAA CAG ACG GTG AGC ΊΤΓ AGT GAC GGT ΉΤ 4080

Asp Pro Gin Gin Gin His Gin Gin Thr Val Ser Phe Ser Asp Gly Phe 1345 1350 1355 1360 GGC CGG TTA CTC CAG AGT TCA GCT CGT CAT GAG TCA GGT GAT GCX: TC5G 4128

Gly Arg Leu Leu Gin Ser Ser Ala Arg His Glu Ser Gly Asp Ala Trp 1365 1370 1375 CM CGT AAA GAG GAT GGC GGG CTG GTC GTG GAT GCA MT GGC GTT CTG 4176

Gin Arg Lys Glu Asp Gly Gly Leu Val Val Asp Ala Asn Gly Val Leu 1380 1385 1390 GTC AGT GCC CCT ACA GAC ACC OGA TGG GCC GTT TCC GGT CGC ACA GM 4224

Val Ser Ala Pro Thr Asp Thr Arg Trp Ala Val Ser Gly Arg Thr Glu 1395 1400 1405 TAT GAC GAC AAA GGC CM CCT GTG CGT ACT TAT CM CCC TAT TTT CTA 4272

Tyr Asp Asp Lys Gly Gin Pro Val Arg Thr Tyr Gin Pro Tyr Phe Leu 1410 1415 1420 AAT GAC TC5G CGT TAC GTT AGT GAT GAC AGC GCA CGA GAT GAC CTG ΤΠΤ 4320

Asn Asp Trp Arg Tyr Val Ser Asp Asp Ser Ala Arg Asp Asp Leu Phe 1425 1430 1435 1440 GCC GAT ACC CAC CIT TAT GAT CCA TTG GGA CXjG GAA TAC AAA GTC ATC 4368

Ala Asp Thr His Leu Tyr Asp Pro Leu Gly Arg Glu Tyr Lys Val He 1445 1450 1455 257- This paper size applies to the Chinese National Standard (CNS) A4 size (210 X 297 mm) Please read the precautions on the back first. I f Page order Printed by the Consumers 'Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs Printed by the Consumers' Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs 509722 A7 B7 V. Invention Description (255) ACT GCT AAG AAA TAT TTG CXJA GAA AAG CTO TAC ACC CCG TGG TTT Απ 4416

Thr Ala Lys Lys Tyr Leu Arg Glu Lys Leu Tyr Thr Pro Trp Phe lie 1460 1465 1470 GTC AGT GAG GAT GAA AAC GAT ACA GCA TCA AGA ACC CCA TAG 4458

Val Ser Glu Asp Glu Asn Asp Thr Ala Ser Arg Thr Pro * 1475 1480 1485 资料 Sequence ID No. 32: (i) Sequence characteristics: (A) Length: 1485 amino acids (B) Type: amino Acid (D) form: Linear (ii) Molecular type: Protein (xi) Sequence description: Sequence identification number 32 (TcaC protein):

Met Gin Asp Ser Pro Glu Val Ser He Thr Thr Leu Ser Leu Pro Lys 1 5 10 15

Gly Gly Gly Ala He Asn Gly Met Gly Glu Ala Leu Asn Ala Ala Gly 20 25 30

Pro Asp Gly Met Ala Ser Leu Ser Leu Pro Leu Pro Leu Ser Thr Gly 35 40 45

Arg Gly Thr Ala Pro Gly Leu Ser Leu He Tyr Ser Asn Ser Ala Gly 50 55 60

Asn Gly Pro Phe Gly He Gly Trp Gin Cys Gly Val Met Ser lie Ser 65 70 75 80

Arg Arg Thr Gin His Gly lie Pro Gin Tyr Gly Asn Asp Asp Thr Phe 85 90 95

Leu Ser Pro Gin Gly Glu Val Met Asn He Ala Leu Asn Asp Gin Gly 100 105 no

Gin Pro Asp lie Arg Gin Asp Val Lys Thr Leu Gin Gly Val Thr Leu 120 125

Pro lie Ser Tyr Thr Val Thr Arg Tyr Gin Ala Arg Gin lie Leu Asp 130 135 140 ~ ^ -------· _ This paper size applies to China National Standard (CNS) A4 (210X297 mm) (please (Please read the notes on the back before filling in this page): Install ·-· Printed by the Consumers' Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs 509722 A7 B7 V. Invention Description (256)

Phe Ser Lys He Glu Tyr Trp Gin Pro Ala Ser Gly Gin Glu Gly Arg 145 150 155 160

Ala Phe Trp Leu He Ser Thr Pro Asp Gly His Leu His lie Leu Gly 165 170 175

Lys Thr Ala Gin Ala Cys Leu Ala Asn Pro Gin Asn Asp Gin Gin He 180 185 190

Ala Gin Trp Leu Leu Glu Glu Thr Val Thr Pro Ala Gly Glu His Val 195 200 205

Ser Tyr Gin Tyr Arg Ala Glu Asp Glu Ala His Cys Asp Asp Asn Glu 210 215 220

Lys Thr Ala His Pro Asn Val Thr Ala Gin Arg Tyr Leu Val Gin Val 225 230 235 240

Asn Tyr Gly Asn lie Lys Pro Gin Ala Ser Leu Phe Val Leu Asp Asn 245 250 255

Ala Pro Pro Ala Pro Glu Glu Trp Leu Phe His Leu Val Phe Asp His 260 265 270

Gly Glu Arg Asp Thr Ser Leu His Thr Val Pro Thr Trp Asp Ala Gly 275 280 285

Thr Ala Gin Trp Ser Val Arg Pro Asp He Phe Ser Arg Tyr Glu Tyr 290 295 300

Gly Phe Glu Val Arg Thr Arg Arg Leu Cys Gin Gin Val Leu Met Phe 305 310 315 320

His Arg Thr Ala Leu Met Ala Gly Glu Ala Ser Thr Asn Asp Ala Pro 325 330 335

Glu Leu Val Gly Arg Leu lie Leu Glu Tyr Asp Lys Asn Ala Ser Val 340 345 350

Thr Thr Leu lie Thr lie Arg Gin Leu Ser His Glu Ser Asp Gly Arg 355 360 365 ___, 9 ^ Q, This paper size applies to China National Standard (CNS) A4 specification (210X297 mm) (Please read the precautions on the back first (Fill in this page again) · Binding · Binding Printed by the Central Consumers Bureau of the Ministry of Economic Affairs, Consumer Cooperatives 509722 A7 B7 Five 'Invention Note (257)

Pro Val Thr Gin Pro Pro Leu Glu Leu Ala Trp Gin Arg Phe Asp Leu 370 375 380

Glu Lys He Pro Thr Trp Gin Arg Phe Asp Ala Leu Asp Asn Phe Asn 385 390 395 400

Ser Gin Gin Arg Tyr Gin Leu Val Asp Leu Arg Gly Glu Gly Leu Pro 405 410 415

Gly Met Leu Tyr Gin Asp Arg Gly Ala Trp Trp Tyr Lys Ala Pro Gin 420 425 430

Arg Gin Glu Asp Gly Asp Ser Asn Ala Val Thr Tyr Asp Lys He Ala 435 440 445

Pro Leu Pro Thr Leu Pro Asn Leu Gin Asp Asn Ala Ser Leu Met Asp 450 455 460

He Asn Gly Asp Gly Gin Leu Asp Trp Val Val Thr Ala Ser Gly lie 465 470 475 480

Arg Gly Tyr His Ser Gin Gin Pro Asp Gly Lys Trp Thr His Phe Thr 485 490 495

Pro lie Asn Ala Leu Pro Val Glu Tyr Phe His Pro Ser He Gin Phe 500 505 510

Ala Asp Leu Thr Gly Ala Gly Leu Ser Asp Leu Val Leu He Gly Pro 515 520 525

Lys Ser Val Arg Leu Tyr Ala Asn Gin Arg Asn Gly Trp Arg Lys Gly 530 535 540

Glu Asp Val Pro Gin Ser Thr Gly He Thr Leu Pro Val Thr Gly Thr 545 550 555 560

Asp Ala Arg Lys Leu Val Ala Phe Ser Asp Met Leu Gly Ser Gly Gin 565 570 575

Gin His Leu Val Glu lie Lys Gly Asn Arg Val Thr Cys Trp Pro Asn 580 585 590 ___- 260- This paper size applies to China National Standard (CNS) A4 (210X 297 mm) (Please read the notes on the back first (Fill in this page again):

* 1T 509722 A7 B7 Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs V. Invention Description (258) Leu Gly His Gly Arg Phe Gly Gin Pro Leu Thr Leu Ser Gly Phe Ser 595 600 605 Gin Pro Glu Asn Ser Phe Asn Pro Glu Arg Leu Phe Leu Ala Asp lie 610 615 620 Asp Gly Ser Gly Thr Thr Asp Leu lie Tyr Ala Gin Ser Gly Ser Leu 625 630 635 640 Leu lie Tyr Leu Asn Gin Ser Gly Asn Gin Phe Asp Ala Pro Leu Thr 645 650 655 Leu Ala Leu Pro Glu Gly Val Gin Phe Asp Asn Thr Cys Gin Leu Gin 660 665 670 Val Ala Asp lie Gin Gly Leu Gly lie Ala Ser Leu lie Leu Thr Val 675 680 685 Pro His lie Ala Pro His His Trp Arg Cys Asp Leu Ser Leu Thr Lys 690 695 700 Pro Trp Leu Leu Asn Val Met Asn Asn Asn Arg Gly Ala His His Thr 705 710 715 720 Leu His Tyr Arg Ser Ser Ala Gin Phe Trp Leu Asp Glu Lys Leu Gin 725 730 735 Leu Thr Lys Ala Gly Lys Ser Pro Ala Cys Tyr Leu Pro Phe Pro Met 740 745 750 His Leu Leu Trp Tyr Thr Glu lie Gin Asp Glu lie Ser Gly Asn Arg 755 760 765 Leu Thr Ser Glu Val Asn Tyr Ser His Gly Val Trp Asp Gly Lys Glu 770 775 780 Arg Glu Phe Arg Gly Phe Gly Cys lie Lys Gin Thr Asp Thr Thr Thr 785 790 795 800 Phe Ser His Gly Thr Ala Pro Glu Gin Ala Ala Pro Ser Leu Ser lie 805 810 815 This paper size applies to China National Standard (CNS) A4 size (210X 297 mm) Ministry of Economy Printed by the Consumer Standards Cooperative of the Central Bureau of Standards 509722 A7 ____. _B7_ V. Description of Invention (259)

Ser Trp Phe Ala Thr Gly Met Asp Glu Val Asp Ser Gin Leu Ala Thr 820 825 830

Glu Tyr Trp Gin Ala Asp Thr Gin Ala Tyr Ser Gly Phe Glu Thr Arg 835 840 845

Tyr Thr Val Trp Asp His Thr Asn Gin Thr Asp Gin Ala Phe Thr Pro 850 855 860

Asn Glu Thr Gin Arg Asn Trp Leu Thr Arg Ala Leu Lys Gly Gin Leu 865 870 875 880

Leu Arg Thr Glu Leu Tyr Gly Leu Asp Gly Thr Asp Lys Gin Thr Val 885 890 895

Pro Tyr Thr Val Ser Glu Ser Arg Tyr Gin Val Arg Ser lie Pro Val 900 905 910

Asn Lys Glu Thr Glu Leu Ser Ala Trp Val Thr Ala lie Glu Asn Arg 915 920 925

Ser Tyr His Tyr Glu Arg He lie Thr Asp Pro Gin Phe Ser Gin Ser 930 935 940 lie Lys Leu Gin His Asp He Phe Gly Gin Ser Leu Gin Ser Val Asp 945 950 955 960 lie Ala Trp Pro Arg Arg Glu Lys Pro Ala Val Asn Pro Tyr Pro Pro 965 970 975

Thr Leu Pro Glu Thr Leu Phe Asp Ser Ser Tyr Asp Asp Gin Gin Gin 980 985 990

Leu Leu Arg Leu Val Arg Gin Lys Asn Ser Trp His His Leu Thr Asp 995 1000 1005

Gly Glu Asn Trp Arg Leu Gly Leu Pro Asn Ala Gin Arg Arg Asp Val 1010 1015 1020

Tyr Thr Tyr Asp Arg Ser Lys He Pro Thr Glu Gly lie Ser Leu Glu 1025 1030 1035 1040 ___- 262- This paper size applies to China National Standard (CNS) A4 specification (210X29 < 7 mm) (Please read the note on the back first (Fill in this page again)

Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 509722 A7 B7 V. Invention Description (26) lie Leu Leu Lys Asp Asp Gly Leu Leu Ala Asp Glu Lys Ala Ala Val 1045 1050 1055

Tyr Leu Gly Gin Gin Gin Thr Phe Tyr Thr Ala Gly Gin Ala Glu Val 1060 1065 1070

Thr Leu Glu Lys Pro Thr Leu Gin Ala Leu Val Ala Phe Gin Glu Thr 1075 1080 1085

Ala Met Met Asp Asp Thr Ser Leu Gin Ala Tyr Glu Gly Val He Glu 1090 1095 1100

Glu Gin Glu Leu Asn Thr Ala Leu Thr Gin Ala Gly Tyr Gin Gin Val 1105 1110 1115 1120

Ala Arg Leu Phe Asn Thr Arg Ser Glu Ser Pro Val Trp Ala Ala Arg 1125 1130 1135

Gin Gly Tyr Thr Asp Tyr Gly Asp Ala Ala Gin Phe Trp Arg Pro Gin 1140 1145 1150

Ala Gin Arg Asn Ser Leu Leu Thr Gly Lys Thr Thr Leu Thr Trp Asp 1155 1160 1165

Thr His His Cys Val lie lie Gin Thr Gin Asp Ala Ala Gly Leu Thr 1170 1175 1180

Thr Gin Ala His Tyr Asp Tyr Arg Phe Leu Thr Pro Val Gin Leu Thr 1185 1190 1195 1200

Asp He Asn Asp Asn Gin His He Val Thr Leu Asp Ala Leu Gly Arg 1205 1210 1215

Val Thr Thr Ser Arg Phe Trp Gly Thr Glu Ala Gly Gin Ala Ala Gly 1220 1225 1230

Tyr Ser Asn Gin Pro Phe Thr Pro Pro Asp Ser Val Asp Lys Ala Leu 1235 1240 1245

Ala Leu Thr Gly Ala Leu Pro Val Ala Gin Cys Leu Val Tyr Ala Val 1250 1255 1260 -263- This paper size applies to Chinese National Standard (CNS) Λ4 specification (210X 297 public holidays) (Please read the notes on the back before filling (This page) ·· 5 · 口 509722 A7 B7 printed by the Consumer Cooperatives of the Central Bureau of Standards of the Ministry of Economic Affairs 5. Description of the Invention (261)

Asp Ser Trp Met Pro Ser Leu Ser Leu Ser Gin Leu Ser Gin Ser Gin 1265 1270 1275 1280

Glu Glu Ala Glu Ala Leu Trp Ala Gin Leu Arg Ala Ala His Met lie 1285 1290 1295

Thr Glu Asp Gly Lys Val Cys Ala Leu Ser Gly Lys Arg Gly Thr Ser 1300 1305 1310

His Gin Asn Leu Thr lie Gin Leu lie Ser Leu Leu Ala Ser lie Pro 1315 1320 1325

Arg Leu Pro Pro His Val Leu Gly lie Thr Thr Asp Arg Tyr Asp Ser 1330 1335 1340

Asp Pro Gin Gin Gin His Gin Gin Thr Val Ser Phe Ser Asp Gly Phe 1345 1350 1355 1360

Gly Arg Leu Leu Gin Ser Ser Ala Arg His Glu Ser Gly Asp Ala Trp 1365 1370 1375

Gin Arg Lys Glu Asp Gly Gly Leu Val Val Asp Ala Asn Gly Val Leu 1380 1385 1390

Val Ser Ala Pro Thr Asp Thr Arg Trp Ala Val Ser Gly Arg Thr Glu 1395 1400 1405

Tyr Asp Asp Lys Gly Gin Pro Val Arg Thr Tyr Gin Pro Tyr Phe Leu 1410 1415 1420

Asn Asp Trp Arg Tyr Val Ser Asp Asp Ser Ala Arg Asp Asp Leu Phe 1425 1430 1435 1440

Ala Asp Thr His Leu Tyr Asp Pro Leu Gly Arg Glu Tyr Lys Val lie 1445 1450 1455

Thr Ala Lys Lys Tyr Leu Arg Glu Lys Leu Tyr Thr Pro Trp Phe lie 1460 1465 1470

Val Ser Glu Asp Glu Asn Asp Thr Ala Ser Arg Thr Pro * 1475 1480 1485 __- 264- This paper is again suitable for China National Standards (CNS) A4 specifications f 21 〇X 297 public holidays) (Please read the note on the back first) Please fill in this page for further information) -Installation.-? · Printed by the Consumers' Cooperative of the Central Standards Bureau of the Ministry of Economy 509722 A7 B7 V. Description of the Invention (262) ⑵ Serial Identification Number No. 33 Information: (i) Sequence characteristics: ( A) Length: 3288 base pairs (B) Type: Nucleic acid (C) Number of strands: Double strand (D) Form: Linear (ii) Molecular type: DNA (genome) (xi) Sequence description: Sequence identification number No. 33 (tcaA gene): ATG GTG ACT GTT ATG CM AAT AAA ΑΤΑ TCA TIT m TCA GGT ACA TCC 48 Met Val Thr Val Met Gin Asn Lys He Ser Phe Leu Ser Gly Thr Ser 15 10 15 GAA CAG CCC CIO CIT GAC GCC GGT TAT CM AAC GTA ΊΤΓ GAT ATC GCA 96

Glu Gin Pro Leu Leu Asp Ala Gly Tyr Gin Asn Val Phe Asp lie Ala 20 25 30 TCA ATC AGC GGG GCT ACT TTC GTT CM TCC GTT CCC ACC CTG CCC GTT 144

Ser lie Ser Arg Ala Thr Phe Val Gin Ser Val Pro Thr Leu Pro Val 35 40 45 AAA GAG GCT CAT ACC GTC TAT CGT CAG GOG CGG CM CGT GCG GM AAT 192

Lys Glu Ala His Thr Val Tyr Arg Gin Ala Arg Gin Arg Ala Glu Asn 50 55 60 CTG AAA TCC CTC TAC CGA GCC TGG CM TKJ CGT CAG GAG CGG GTT Απ 240

Leu Lys Ser Leu Tyr Arg Ala Trp Gin Leu Arg Gin Glu Pro Val lie 65 70 75 80 AAA GGG CTG GCT AAA CIT AAC GTA CM TCC MC GTT TCT GTG CTT CM 288

Lys Gly Leu Ala Lys Leu Asn Leu Gin Ser Asn Val Ser Val Leu Gin 85 90 95 GAT GCTTTG GTA GAG AAT Απ GGC GGT GAT GGG GAT TTC AGC GAT m 336

Asp Ala Leu Val Glu Asn He Gly Gly Asp Gly Asp Phe Ser Asp Leu 100 105 110 ATG AAC CGT GCC AGT CM TAT GCT GAC (O GCC TCT Απ CM TCC CTA 384 _-26S > This paper size applies to Chinese national standards (CNS ) A4 size (210 X; 297 mm) (Please read the notes on the back before filling this page)

Printed by the Consumer Cooperatives of the Central Bureau of Standards of the Ministry of Economic Affairs 509722 A7 B7 V. Description of Invention (263)

Met Asn Arg Ala Ser Gin Tyr Ala Asp Ala Ala Ser lie Gin Ser Leu 115 120 125 ΉΤ TCA COG GGC OGT TAT GCT TCC GCA CTC TAC AGA On GCT AAA GAT 432

Phe Ser Pro Gly Arg Tyr Ala Ser Ala Leu Tyr Arg Val Ala Lys Asp 130 135 140 CTG CAT AAA TCA GAT TCC AGT TTG CAT Απ GAT AAT CGC CGC GCT GAT 480

Leu His Lys Ser Asp Ser Ser Leu His He Asp Asn Arg Arg Ala Asp 145 150 155 160 CTG AAG GAT CTG ΑΤΑ TTA AGC GAA ACG ACG ATG AAT AAA GAG GTC ACT 528

Leu Lys Asp Leu He Leu Ser Glu Thr Thr Met Asn Lys Glu Val Thr 165 170 175 TGC CTT GAT ATC TTG TTG GAT GTG CTA CM AM GGC GGT AAA GAT Απ 576

Ser Leu Asp lie Leu Leu Asp Val Leu Gin Lys Gly Gly Lys Asp He 180 185 190 ACT GAG CTG TCC GGC GCA TTC TTC CCA ATC AGG TTA CCT TAT GAC GAT 624

Thr Glu Leu Ser Gly Ala Phe Phe Pro Met Thr Leu Pro Tyr Asp Asp 195 200 205 CAT CTG TCG CM ATC GAT TCC GCT m TOG GCA CM GCC AGA ACG CTG 672

His Leu Ser Gin lie Asp Ser Ala Leu Ser Ala Gin Ala Arg Thr Leu 210 215 220 MC GGT GTG TAT AAT ACT TTG ACA GAT ACC ACG GCA CM GCG GIT TCA 720

Asn Gly Val Trp Asn Thr Leu Thr Asp Thr Thr Ala Gin Ala Val Ser 225 230 235 240 GM CM ACC AGT AAT ACG MT ACA CGC AAA CTG TTC GCT GCC CM GAT 768

Glu Gin Thr Ser Asn Thr Asn Thr Arg Lys Leu Phe Ala Ala Gin Asp 245 250 255 GGT AAT CM GAT ACA ΤΓΤ ΤΓΤ TCC GGA AAC ACT m TAT TTC AAA GCG 816

Gly Asn Gin Asp Thr Phe Phe Ser Gly Asn Thr Phe Tyr Phe Lys Ala 260 265 270 GTG GGA TTC AGC GGG CM CCT ATC GTT TAC CTG TCA CAG TAC ACC AGC 864

Val Gly Phe Ser Gly Gin Pro Met Val Tyr Leu Ser Gin Tyr Thr Ser 275 280 285 -266- This paper size applies to China National Standard (CNS) A4 size (210X 297 mm) (Please read the precautions on the back before Fill out this page) Order 509722 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs A7 __B7___ V. Invention Description (264) GOJ MC (X3C ATT GTC GGC GCA CM TKJ Απ GCA GGT AAT CCk GAC CM 912 Gly Asn Gly lie Val Gly Ala Gin Leu He Ala Gly Asn Pro Asp Gin 290 295 300 GCC GCC GCC GCA ATA GTC GCA CCG TTG AM CTC ACT TGG TCA ATG GCA 960 Ala Ala Ala Ala He Val Ala Pro Leu Lys Leu Thr Trp Ser Met Ala 305 310 315 320 AAA CAG TGT TAC TAC CTC GTC GCT CCC GAT GGT ACA ACG ATG GGA GAC 1008 Lys Gin Cys Tyr Tyr Leu Val Ala Pro Asp Gly Thr Thr Met Gly Asp 325 330 335 GGT AAT GTT CTG ACC GGC TGT TTC m AGA GGC AAC AGC CCA ACT MC 1056 Gly Asn Val Leu Thr Gly Cys Phe Leu Arg Gly Asn Ser Pro Thr Asn 340 345 350 CCXj GAT AAA GAC GGT Απ ΉΤ GCT CAG GTA GCC AAC AAA TCA GGC AGT 1104 Pro Asp Lys Asp Gly He Phe Ala Gin Val AlaAsn Lys Ser Gly Ser 355 360 365 ACT CAG CCT TTG CCA A (X: TTC CAT CTG COG GTC ACA CTG GAA CAC AGC 1152 Thr Gin Pro Leu Pro Ser Phe His Leu Pro Val Thr Leu Glu His Ser 370 375 380 GAG AAT AAA GAT CAG TAC TAT CTG AAA ACA GAG CAG GGT TAT ATC AOG 1200 Glu Asn Lys Asp Gin Tyr Tyr Leu Lys Thr Glu Gin Gly Tyr lie Thr 385 390 395 400 GTA GAT AGT TCC GGA CAG TCA MT TGG AAA MC GCG CTG GTT ATC 1248 Val Asp Ser Ser Gly Gin Ser Asn Trp Lys Asn Ala Leu Val He Asn 405 410 415 GGG ACA AM GAC MG GGG CTG mm ACC Ή TGC AGC GAT AGC TCA 1296 Gly Thr Lys Asp Lys Gly Leu Leu Leu Thr Phe Cys Ser Asp Ser Ser 420 425 430 GGC ACT COG ACA AAC CCT GAT GAT (ΠΌ Απ CCT CCC GCT ATC AAT GAT 1344 Gly Thr Pro Thr Asn Pro Asp Asp Val lie Pro Pro Ala lie Asn Asp 435 440 445 Απ CCA TOG CCG CCA GCC CGC GM ACA CTG TCA CTG ACG CCG GTC AGT 1392 (Please read the notes on the back before filling out this page): Binding __________- 267- This paper size applies to China National Standard (CNS) A4 specification (210X 297 mm) Economy Central standard Printed by the Consumer Bureau of the Associate Bureau 509722 A7 __. B7 _ V. Description of Invention (265)

He Pro Ser Pro Pro Ala Arg Glu Thr Leu Ser Leu Thr Pro Val Ser 450 455 460 TAT CAA TTG ATG ACC AAT CCG GCA CCG ACA GAA GAT GAT Απ ACC AAC 1440

Tyr Gin Leu Met Thr Asn Pro Ala Pro Thr Glu Asp Asp lie Thr Asn 465 470 475 480 CAT TAT GGT TIT MC GGC GCT AGC ΤΓΑ CGG GCT TCT CCA TTG TCA ACC 1488

His Tyr Gly Phe Asn Gly Ala Ser Leu Arg Ala Ser Pro Leu Ser Thr 485 490 495 AGC GAG TTG ACC AGC AAA CTG MT TCT ATC GAT ACT TTC TGT GAG MG 1536

Ser Glu Leu Thr Ser Lys Leu Asn Ser He Asp Thr Phe Cys Glu Lys 500 505 510 ACC CX3G m AGC TTC AAT CAG m ATG GAT TTG ACC GCT CAG CAA TCT 1584

Thr Arg Leu Ser Phe Asn Gin Leu Met Asp Leu Thr Ala Gin Gin Ser 515 520 525 TAC ACT CM AGC AGC Απ GAT GCG AAA GCA GCC AGC CGC TAT GTT OGT 1632

Tyr Ser Gin Ser Ser He Asp Ala Lys Ala Ala Ser Arg Tyr Val Arg 530 535 540 TTT GGG GM ACC ACC CCA ACC CGC GTC MT GTC TAC GGT GCC GCT TAT 1680

Phe Gly Glu Thr Thr Pro Thr Arg Val Asn Val Tyr Gly Ala Ala Tyr 545 550 555 560 CTG AAC AGC ACA CTG GCA GAC GOG GCT GAT GGT CAA. TAT CTG TGG ΑΠ 1728

Leu Asn Ser Thr Leu Ala Asp Ala Ala Asp Gly Gin Tyr Leu Trp lie 565 570 575 CAG ACT GAT GGC AAG AGC CTA AAT TTC ACT GAC GAT ACXJ GTA GTC GCC 1776

Gin Thr Asp Gly Lys Ser Leu Asn Phe Thr Asp Asp Thr Val Val Ala 580 585 590 m GCC GGT CGC GCT GM MG CTG GTA CGT m TCA TCC CAG ACC GGG 1824

Leu Ala Gly Arg Ala Glu Lys Leu Val Arg Leu Ser Ser Gin Thr Gly 595 600 605 CTA TCA TTT GM GM TTG GAC TGG CTG Απ GCC AAT GCC ACT CGT AGT 1872

Leu Ser Phe Glu Glu Leu Asp Trp Leu He Ala Asn Ala Ser Arg Ser 610 615 620 _________ > 268- This paper size applies to China National Standard (CNS) A4 specification (210X 297 mm) (Please read the precautions on the back first (Fill in this page again) Order 509722 A7 ____B7 printed by the Employees' Cooperative of the Central Standards Bureau of the Ministry of Economy V. Invention Description (266) GTG CXXJ GAC CAC CAC GAC AAA ATT GTG CTG GAT AAG (XG GTC CTT GAA 1920

Val Pro Asp His His Asp Lys He Val Leu Asp Lys Pro Val Leu Glu 625 630 635 640 GCA CTG GCA GAG TAT GTC AC5C CTA AAA CAG CGC TAT GGG CTT GAT GCC 1968

Ala Leu Ala Glu Tyr Val Ser Leu Lys Gin Arg Tyr Gly Leu Asp Ala 645 650 655 AAT ACC TTT GCXJ ACC TTC Απ AGT GCA GTA AAT (ΓΓ TAT ACG CCA GAT 2016

Asn Thr Phe Ala Thr Phe lie Ser Ala Val Asn Pro Tyr Thr Pro Asp 660 665 670 CAG ACA CCC AGT ITC TAT GM ACC GCT TTG CGC TCT GCC GAC GGT AAT 2064

Gin Thr Pro Ser Phe Tyr Glu Thr Ala Phe Arg Ser Ala Asp Gly Asn 675 680 685 CAT GTC Απ GCG CTA GGT ACA GAG GTG AAA TAT GCA GAA AAT GAG CAG 2112

His Val lie Ala Leu Gly Thr Glu Val Lys Tyr Ala Glu Asn Glu Gin 690 695 700 GAT GAG m GCC GCC ATA TGC TGC AAA GCA TTG GGT GTC ACC AGT GAT 2160

Asp Glu Leu Ala Ala lie Cys Cys Lys Ala Leu Gly Val Thr Ser Asp 705 710 715 720 GAA CTG CTC CGT Απ GGT CGC TAT TGC TTC GGT MT GCA GGC AGT TIT 2208

Glu Leu Leu Arg He Gly Arg Tyr Cys Phe Gly Asn Ala Gly Ser Phe 725 730 735 ACC TTG GAT GAA TAT ACC GCC AGT CAG TTG TAT CGC TO GGC GCC Απ 2256

Thr Leu Asp Glu Tyr Thr Ala Ser Gin Leu Tyr Arg Phe Gly Ala lie 740 745 750 CCC CGT TTG TTT GGG CTG ACA ΉΤ GCC CM GCC GM ATT ΤΓΑ TGG CGT 2304

Pro Arg Leu Phe Gly Leu Thr Phe Ala Gin Ala Glu He Leu Trp Arg 755 760 765 CTG ATG GAA GGC GGA AAA GAT ATC m TTG CM CAG m GGT CAG GCA 2352

Leu Met Glu Gly Gly Gly Lys Asp He Leu Leu Gin Gin Leu Gly Gin Ala 770 775 780 AAA TCC CTG CM CCA CTG GCT ATT ΤΓ CGC CGT ACC GAG CAG GTG CTG 2400 -269- This paper conforms to Chinese National Standard (CNS) A4 specifications (210X; 297 mm) (Please read the precautions on the back before filling out this page), order 509722 printed by the Consumers' Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs A7 __B7 V. Description of the invention (267) Lys Ser Leu Gin Pro Leu Ala He Leu Arg Arg Thr Glu Gin Val Leu 785 790 795 800 GAT TGG ATG TCXj TCC GTA AAT CTA AGT CTG ACT TAT CTG CM GGG ATG 2448 Asp Trp Met Ser Ser Val Asn Leu Ser Leu Thr Tyr Leu Gin Gly Met 805 810 815 GTA AGT ACG CM TGG AGC GC3T ACC GCC ACC GCT GAG ATG TO AAT TTC 2496 Val Ser Thr Gin Trp Ser Gly Thr Ala Thr Ala Glu Met Phe Asn Phe 820 825 830 TTG GAA AAC GTT TGT GAC AGC (ΠΌ AAT AGT CAA GCT GCC GCC ACT AAA GAA 2544 Leu Glu Asn Val Cys Asp Ser Val Asn Ser Gin Ala Ala Thr Lys Glu 835 840 845 ACA ATG GAT TCG GCG m CAG CAG AAA GTG CTG CGG GCG CTA AGC GCC 2592 Thr Met Asp Ser Ala Leu Gin Gin Lys Val Leu Arg Ala Leu Ser Ala 850 855 860 GGT TTC GGC Απ MG AGC MT GTG ATG GGT ATC GTC ACC TTC TGG CTG 2640 Gly Phe Gly He Lys Ser Asn Val Met Gly lie Val Thr Phe Trp Leu 865 870 875 880 GAG AAA ATC ACA ATC GCST AGT GAT AAT CCT TIT ACA TTG GCA AAC TAC 2688 Glu Lys He Thr He Gly Ser Asp Asn Pro Phe Thr Leu Ala Asn Tyr 885 890 895 TGG CAT GAT ATT CM ACC CTG ΤΊΤ AGC CAT GAC MT GCC ACG m GAG 2736 Trp His Asp He Gin Thr Leu Phe Ser His Asp Asn Ala Thr Leu Glu 900 905 910 TCC m CM ACC GAC ACT TCT CTG GTA Απ GCT ACT CAG CM CTT AGC 2784 Ser Leu Gin Thr Asp Thr Ser Leu Val He Ala Thr Gin Gin Leu Ser 915 920 925 CAG CTA GTG ΓΓ ATT GTG AAA TGG CTG AGC CTG ACC GAG CAG GAT CTG 2832 Gin Leu Val Leu He Val Lys Trp Leu Ser Leu Thr Glu Gin Asp Leu 930 935 940 CAA m CTG ACA ACC TAT CCC GAA CGT m ATC AAC GGC ATC AOG AAT 2880 Gin Leu Leu Thr Thr Tyr Pro Glu Arg Leu He Asn Gly lie Thr Asn 945 950 955 960 (Please read the notes on the back before filling this page) Order # · — _-270- ^ Paper size applies National Standard (CNS) A4 (21 OX 297 mm) Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economy 509722 A7 __B7_ V. Description of Invention (268) GTT CCT GTA CCC MT COG GAG CTA m CTC AOG CTA TCA CGT ΤΊΤ MG 2928

Val Pro Val Pro Asn Pro Glu Leu Leu Leu Thr Leu Ser Arg Phe Lys 965 970 975 CAG TGG GAA ACT CAA GTC ACC GTT TCC CGT GAT GAA GCG ATG CGC TGT 2976

Gin Trp Glu Thr Gin Val Thr Val Ser Arg Asp Glu Ala Met Arg Cys 980 985 990 TTC GAT CAA m AAT GCC AAT GAT ATG AOG ACT GAA AAT GCA GCjT TCA 3024

Phe Asp Gin Leu Asn Ala Asn Asp Met Thr Thr Glu Asn Ala Gly Ser 995 1000 1005 CTG ATC GCC ACA TTG TAT GAG ATG GAT AM 〇ιΓ ACG GGA GCG CAA GTT 3072

Leu lie Ala Thr Leu Tyr Glu Met Asp Lys Gly Thr Gly Ala Gin Val 1010 1015 1020 MT ACC TTG CTA m GGT GAA MT MC TGG CCG AAA AGT ΉΤ ACC TCT 3120

Asn Thr Leu Leu Leu Gly Glu Asn Asn Trp Pro Lys Ser Phe Thr Ser 1025 1030 1035 1040 CTC TGG CM CIT CTG ACC TGG m CGC GTC GGG CAA AGA CTG MT GTC 3168

Leu Trp Gin Leu Leu Thr Trp Leu Arg Val Gly Gin Arg Leu Asn Val 1045 1050 1055 GGT AGT ACC ACT CTG GGC MT CTG TTG TCC ATG ATG CM GCA GAC OCT 3216

Gly Ser Thr Thr Leu Gly Asn Leu Leu Ser Met Met Gin Ala Asp Pro 1060 1065 1070 GCT GCC GAG AGT AGC GCT ΊΓΓΑ TTG GCA TCA GTA GCC CAA AAC TTA AGT 3264

Ala Ala Glu Ser Ser Ala Leu Leu Ala Ser Val Ala Gin Asn Leu Ser 1075 1080 1085 GCC GCA ATC AGC AAT CGT CAG TAA 3288

Ala Ala He Ser Asn Arg Gin *** 1090 1095 资料 Data of sequence identification number 34: ①Sequence characteristics: (A) Height: 1095 amino acids (B) Type: amino acid ____- 271- present Paper size applies Chinese National Standard (CNS) A4 specification (210X297 mm) (please read the precautions on the back before filling this page) Order 509722 A7 ______ B7 V. Description of the invention (269) (C) Form: Linear (ii) ) Molecular type: protein (xi) sequence description: sequence identification number 34 (TcaA protein, · characteristic start and end description 254 267 sequence identification number 15 254 492 TcaAii wins Met Val Thr Val Met Gin Asn Lys lie Ser Phe Leu Ser Gly Thr Ser 15 10 15 Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs

Glu Gin Pro Leu Leu Asp Ala Gly Tyr Gin Asn Val Phe Asp lie Ala 20 25 30 Ser lie Ser Arg Ala Thr Phe Val Gin Ser Val Pro Thr Leu Pro Val 35 40 45 Lys Glu Ala His Thr Val Tyr Arg Gin Ala Arg Gin Arg Ala Glu Asn 50 55 60 Leu Lys Ser Leu Tyr Arg Ala Trp Gin Leu Arg Gin Glu Pro Val lie 65 70 75 80 Lys Gly Leu Ala Lys Leu Asn Leu Gin Ser Asn Val Ser Val Leu Gin 85 90 95 Asp Ala Leu Val Glu Asn He Gly Gly Asp Gly Asp Phe Ser Asp Leu 100 105 110 Met Asn Arg Ala Ser Gin Tyr Ala Asp Ala Ala Ser He Gin Ser Leu 115 120 Phe Ser Pro Gly Arg Tyr Ala Ser Ala Leu Tyr Arg Val Ala Lys Asp 130 135 140 Leu His Lys Ser Asp Ser Ser Leu His He Asp Asn Arg Arg Ala Asp 145 150 155 160 Leu Lys Asp Leu lie Leu Ser Glu Thr Thr Met Asn Lys Glu Val Thr 165 170 1V5 National Standard (CNS) A4 Specification (210X 297mm) (Please read the notes on the back before filling out this page}; Packing. Order € 509722 A7 B7 V. Description of Invention (27〇)

Ser Leu Asp He Leu Leu Asp Val Leu Gin Lys Gly Gly Lys Asp He 180 185 190

Thr Glu Leu Ser Gly Ala Phe Phe Pro Met Thr Leu Pro Tyr Asp Asp 195 200 205

His Leu Ser Gin lie Asp Ser Ala Leu Ser Ala Gin Ala Arg Thr Leu 210 215 220

Asn Gly Val Trp Asn Thr Leu Thr Asp Thr Thr Ala Gin Ala Val Ser 225 230 235 240

Glu Gin Thr Ser Asn Thr Asn Thr Arg Lys Leu Phe Ala Ala Gin Asp 245 250 255

Gly Asn Gin Asp Thr Phe Phe Ser Gly Asn Thr Phe Tyr Phe Lys Ala 260 265 270

Val Gly Phe Ser Gly Gin Pro Met Val Tyr Leu Ser Gin Tyr Thr Ser 275 280 285

Gly Asn Gly He Val Gly Ala Gin Leu He Ala Gly Asn Pro Asp Gin 290 295 300

Ala Ala Ala Ala Ala He Val Ala Pro Leu Lys Leu Thr Trp Ser Met Ala 305 310 315 320

Lys Gin Cys Tyr Tyr Leu Val Ala Pro Asp Gly Thr Thr Met Gly Asp 325 330 335

Gly Asn Val Leu Thr Gly Cys Phe Leu Arg Gly Asn Ser Pro Thr Asn 340 345 350

Pro Asp Lys Asp Gly lie Phe Ala Gin Val Ala Asn Lys Ser Gly Ser 355 360 365

Thr Gin Pro Leu Pro Ser Phe His Leu Pro Val Thr Leu Glu His Ser 370 375 380

Glu Asn Lys Asp Gin Tyr Tyr Leu Lys Thr Glu Gin Gly Tyr lie Thr 385 390 395 400 This paper size applies to China National Standard (CNS) A4 (210X 297 mm) Please read the precautions on the back before i Economy Printed by the Consumer Standards Cooperative of the Ministry of Standards of the People's Republic of China Printed by the Consumer Standards Cooperative of the Ministry of Economy of the People's Republic of China 509722 A 7 B7 V. Description of the Invention

Val Asp Ser Ser Gly Gin Ser Asn Trp Lys Asn Ala Leu Val He Asn 405 410 415

Gly Thr Lys Asp Lys Gly Leu Leu Leu Thr Phe Cys Ser Asp Ser Ser 420 425 430

Gly Thr Pro Thr Asn Pro Asp Asp Val He Pro Pro Ala lie Asn Asp 435 440 445 lie Pro Ser Pro Pro Ala Arg Glu Thr Leu Ser Leu Thr Pro Val Ser 450 455 460

Tyr Gin Leu Met Thr Asn Pro Ala Pro Thr Glu Asp Asp lie Thr Asn 465 470 475 480

His Tyr Gly Phe Asn Gly Ala Ser Leu Arg Ala Ser Pro Leu Ser Thr 485 490 W4 * 495

Ser Glu Leu Thr Ser Lys Leu Asn Ser lie Asp Thr Phe Cys Glu Lys 500 505 510

Thr Arg Leu Ser Phe Asn Gin Leu Met Asp Leu Thr Ala Gin Gin Ser 515 520 525

Tyr Ser Gin Ser Ser lie Asp Ala Lys Ala Ala Ser Arg Tyr Val Arg 530 535 540

Phe Gly Glu Thr Thr Pro Thr Arg Val Asn Val Tyr Gly Ala Ala Tyr 545 550 555 560

Leu Asn Ser Thr Leu Ala Asp Ala Ala Asp Gly Gin Tyr Leu Trp He 565 570 575

Gin Thr Asp Gly Lys Ser Leu Asn Phe Thr Asp Asp Thr Val Val Ala 580 585 590

Leu Ala Gly Arg Ala Glu Lys Leu Val Arg Leu Ser Ser Gin Thr Gly 595 600 605

Leu Ser Phe Glu Glu Leu Asp Trp Leu lie Ala Asn Ala Ser Arg Ser 610 615 620 _-9.74- This paper size is applicable to China National Standard (CNS) A4 specification (210X 297 mm) (Please read the first.M read the back (Please fill in this page for the matters needing attention)

、 1T 509722 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs A7 __B7 _ V. Invention Description (272) Val Pro Asp His His Asp Lys lie Val Leu Asp Lys Pro Val Leu Glu 625 630 635 640 Ala Leu Ala Glu Tyr Val Ser Leu Lys Gin Arg Tyr Gly Leu Asp Ala 645 650 655 Asn Thr Phe Ala Thr Phe He Ser Ala Val Asn Pro Tyr Thr Pro Asp 660 665 670 Gin Thr Pro Ser Phe Tyr Glu Thr Ala Phe Arg Ser Ala Asp Gly Asn 675 680 685 His Val lie Ala Leu Gly Thr Glu Val Lys Tyr Ala Glu Asn Glu Gin 690 695 700 Asp Glu Leu Ala Ala He Cys Cys Lys Ala Leu Gly Val Thr Ser Asp 705 710 715 720 Glu Leu Leu Arg He Gly Arg Tyr Cys Phe Gly Asn Ala Gly Ser Phe 725 730 735 Thr Leu Asp Glu Tyr Thr Ala Ser Gin Leu Tyr Arg Phe Gly Ala lie 740 745 750 Pro Arg Leu Phe Gly Leu Thr Phe Ala Gin Ala Glu lie Leu Trp Arg 755 760 765 Leu Met Glu Gly Gly Lys Asp lie Leu Leu Gin Gin Leu Gly Gin Ala 770 775 780 Lys Ser Leu Gin Pro Leu Ala lie Leu Arg Arg Thr Glu Gin Val Leu 785 790 795 800 Asp Trp Met Ser Ser Val Asn Leu Ser Leu Thr Tyr Leu G in Gly Met 805 810 815 Val Ser Thr Gin Trp Ser Gly Thr Ala Thr Ala Glu Met Phe Asn Phe 820 825 830 Leu Glu Asn Val Cys Asp Ser Val Asn Ser Gin Ala Ala Thr Lys Glu 835 840 845 (Please read the back first (Please fill in this page again)

This paper size applies Chinese National Standard (CNS) M specification (210x 297 mm) 509722 A7 B7 V. Description of the invention (273)

Thr Met Asp Ser Ala Leu Gin Gin Lys Val Leu Arg Ala Leu Ser Ala 850 855 860

Gly Phe Gly He Lys Ser Asn Val Met Gly lie Val Thr Phe Trp Leu 865 870 875 880

Glu Lys lie Thr He Gly Ser Asp Asn Pro Phe Thr Leu Ala Asn Tyr 885 890 895

Trp His Asp lie Gin Thr Leu Phe Ser His Asp Asn Ala Thr Leu Glu 900 905 910

Ser Leu Gin Thr Asp Thr Ser Leu Val lie Ala Thr Gin Gin Leu Ser 915 920 925

Gin Leu Val Leu lie Val Lys Trp Leu Ser Leu Thr Glu Gin Asp Leu 930 935 940

Gin Leu Leu Thr Thr Tyr Pro Glu Arg Leu lie Asn Gly lie Thr Asn 945 950 955 960

Val Pro Val Pro Asn Pro Glu Leu Leu Leu Thr Leu Ser Arg Phe Lys 965 970 975

Gin Trp Glu Thr Gin Val Thr Val Ser Arg Asp Glu Ala Met Arg Cys 980 985 990

Phe Asp Gin Leu Asn Ala Asn Asp Met Thr Thr Glu Asn Ala Gly Ser 995 1000 1005 Printed by the Consumers' Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs ϋϋ ^^ i_i ml I mV βιΒϋ ϋϋ n ^ i (Please read the note on the back first (Fill in this page again)

Leu He Ala Thr Leu Tyr Glu Met Asp Lys Gly Thr Gly Ala Gin Val 1010 1015 1020

Asn Thr Leu Leu Leu Gly Glu Asn Asn Trp Pro Lys Ser Phe Thr Ser 1025 1030 1035 1040

Leu Trp Gin Leu Leu Thr Trp Leu Arg Val Gly Gin Arg Leu Asn Val 1045 1050 1055

Gly Ser Thr Thr Leu Gly Asn Leu Leu Ser Met Met Gin Ala Asp Pro 1060 1065 1070 ------- 276- This paper size applies to China National Standard (CNS) A4 specifications (210 X 297 mm) 509722 Ministry of Economic Affairs Printed by A7 B7 of the Consumer Standards Cooperative of the Central Bureau of Standards 5. Description of Invention (274)

Ala Ala Glu Ser Ser Ala Leu Leu Ala Ser Val Ala Gin Asn Leu Ser 1075 1080 1085

Ala Ala He Ser Asn Arg Gin *** 1090 1095 资料 Sequence ID No. 35: (i) Sequence characteristics: (A) Length: 603 amino acids (B) Type: amino acid (C) Form : Linear (ii) molecular type: protein (xi) sequence description: sequence identification number 35 (TcaAHi protein):

Pro Leu Ser Thr Ser Glu Leu Thr Ser Lys Leu Asn Ser He Asp Thr 15 10 15

Phe Cys Glu Lys Thr Arg Leu Ser Phe Asn Gin Leu Met Asp Leu Thr 20 25 30

Ala Gin Gin Ser Tyr Ser Gin Ser Ser He Asp Ala Lys Ala Ala Ser 35 40 45

Arg Tyr Val Arg Phe Gly Glu Thr Thr Pro Thr Arg Val Asn Val Tyr 50 55 60

Gly Ala Ala Tyr Leu Asn Ser Thr Leu Ala Asp Ala Ala Asp Gly Gin 65 70 75 80

Tyr Leu Trp lie Gin Thr Asp Gly Lys Ser Leu Asn Phe Thr Asp Asp 85 90 95

Thr Val Val Ala Leu Ala Gly Arg Ala Glu Lys Leu Val Arg Leu Ser 100 105 110

Ser Gin Thr Gly Leu Ser Phe Glu Glu Leu Asp Trp Leu He Ala Asn 115 120 125

Ala Ser Arg Ser Val Pro Asp His His Asp Lys lie Val Leu Asp Lys -277-This paper size is applicable to China National Standard (CNS) A4 size (210X 297 mm) (Please read the precautions on the back before filling in this page)

509722 Printed by A7 __B7 of the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 5. Description of the Invention (275) 130 135 140 Pro Val Leu Glu Ala Leu Ala Glu Tyr Val Ser Leu Lys Gin Arg Tyr 145 150 155 160 Gly Leu Asp Ala Asn Thr Phe Ala Thr Phe He Ser Ala Val Asn Pro 165 170 175 Tyr Thr Pro Asp Gin Thr Pro Ser Phe Tyr Glu Thr Ala Phe Arg Ser 180 185 190 Ala Asp Gly Asn His Val lie Ala Leu Gly Thr Glu Val Lys Tyr Ala 195 200 205 Glu Asn Glu Gin Asp Glu Leu Ala Ala He Cys Cys Lys Ala Leu Gly 210 215 220 Val Thr Ser Asp Glu Leu Leu Arg He Gly Arg Tyr Cys Phe Gly Asn 225 230 235 240 Ala Gly Arg Phe Thr Leu Asp Glu Tyr Thr Ala Ser Gin Leu Tyr Arg 245 250 255 Phe Gly Ala He Pro Arg Leu Phe Gly Leu Thr Phe Ala Gin Ala Glu 260 265 270 lie Leu Trp Arg Leu Met Glu Gly Gly Lys Asp He Leu Leu Gin Gin Gin 275 280 285 Xxx Gly Gin Ala Lys Ser Leu Gin Pro Leu Ala lie Leu Arg Arg Thr 290 295 300 Glu Gin Val Leu Asp Trp Met Ser Pro Val Asn Leu Ser Leu Thr Tyr 305 310 315 320 Leu Gin Gly Met Val Ser Thr Gin Trp Ser Gly Thr Ala Thr Ala Glu 325 330 335 Met Phe Asn Phe Leu Glu Asn Val Cys Asp Ser Val Asn Ser Gin Ala 340 345 350 Xxx Thr Lys Glu Thr Met Asp Ser Ala Leu Gin Gin Lys Val Leu Arg Φ t (Please read first on the back Attention ^ # 1¾ • Please fill in the items again · Load II page)

、 1T -278-This paper size is applicable to China National Standard (CNS) A4 (210X 297 mm) 509722 A7 __B7 V. Description of the invention (276) 355 360 365

Ala Leu Ser Ala Gly Phe Gly lie Lys Ser Asn Val Met Gly lie Val 370 375 380

Thr Phe Trp Leu Glu Lys He Thr He Gly Arg Asp Asn Pro Phe Thr 385 390 395 400

Leu Ala Asn Tyr Trp His Asp lie Gin Thr Leu Phe Ser His Asp Asn 405 410 415

Ala Thr Leu Glu Ser Leu Gin Thr Asp Thr Ser Leu Val lie Ala Thr 420 425 430

Gin Gin Leu Ser Gin Leu Val Leu lie Val Lys Trp Val Ser Leu Thr 435 440 445

Glu Gin Asp Leu Gin Leu Leu Thr Thr Tyr Pro Glu Arg Leu lie Asn 450 455 460

Gly He Thr Asn Val Pro Val Pro Asn Pro Glu Leu Leu Leu Thr Leu 465 470 475 480

Ser Arg Phe Lys Gin Trp Glu Thr Gin Val Thr Val Ser Arg Asp Glu 485 490 495

Ala Met Arg Cys Phe Asp Gin Leu Asn Ala Asn Asp Met Thr Thr Glu 500 505 510

Asn Ala Gly Ser Leu lie Ala Thr Leu Tyr Glu Met Asp Lys Gly Thr 515 520 525 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling out this page)

Gly Ala Gin Val Asn Thr Leu Leu Leu Gly Glu Asn Asn Trp Pro Lys 530 535 540

Ser Phe Thr Ser Leu Trp Gin Leu Leu Thr Trp Leu Arg Val Gly Gin 545 550 555 560

Arg Leu Asn Val Gly Ser Thr Thr Leu Gly Asn Leu Leu Ser Met Met 565 570 575

Gin Ala Asp Pro Ala Ala Glu Ser Ser Ala Leu Leu Ala Ser Val Ala _____ m = _ This paper size applies to Chinese National Standard (CNS) A4 specification (210X29? Mm) 509722 A7 B7 V. Description of the invention (277) 580 585 590

Gin Asn Leu Ser Ala Ala lie Ser Asn Arg Gin * 595 600 资料 Sequence ID No. 36: (i) Sequence characteristics: (A) Length: 2557 base pairs (B) Type: Nucleic acid (C) Form : Linear (ii) Molecular type: DNA (genome) (xi) Sequence description: Sequence identification number 36 (tcdA fragment): printed by GMTTCGGCT TGCGTTTAAT ATTGATGATG TUTCXOCTT CCGCCTCCTT AAAATTACCXj 60 AOCATGATAA TAAAGATGGA ΑΑΑΑΊΤΑΑΑΑ ATAACCTAAA GMIUTTTCX: ΑΑΉΤΑΤΑΤΑ 120 TTGGAAAATT ACTGGCAGAT ATTCATCAAT TAACCATTGA TGAACKjGAT mTTACTGA 180 TTGCOGTAGG TGMGGAAAA ΑΟΜΠΤΑΤ CCGCTATCAG TGATMGCM TTGGCTACCC 240 TCATCAGAAA ACTCMTACT ATTACCAGCT GGCTACATAC ACAGMGTGG AGTGTATTCC 300 AGCTATITAT CATCACCTCC ACCAGCTATA ACAAAACGCT MCGCCTGM AHAAGMIT 360 TGCTGGATAC OiTCTACCAC GGTTTACAAG GHTTGATAA AGACAAAGCA GATTTGCTAC 420 ATGTCATGGC GCCCTATAn GCGGCCACCT TGCMTTATC ATCGGAAAAT GT0GCCCACT 480 OMACrCCr TTGGGCAGAT AAC5TTACAGC CCXJGCXjAOGG OGCAATGACA GCAGAGGGAN 540 TCTGCjGACTG GTTGAATACT AAGT ATACGC amTCATC GGAAGCCGTA GAAACXjCAGG 600 MCATATCGT TCAGTATTGT CAGGCTCTGG CACMTTGGA MTGGTTTAC CAnCCACOG 660 GCATCMCGA MACGCCTTC CGTCTATITG TGACAAAACC AGAGATGTTT GGCGCTGCM 720 CTGGAGCAGC GCCCGCGCAT GATGCCCTTT CACTGAmT GCTGACACGT TITGCGGAW 780 GGGTGMCGC ACTAGGCGM AMGCGTCCT CGGTGCTAGC GGCATITGM GCTMCTCGT 840 TMCGGCAGA ACMCTGGCT GATGCCATGA ATCTTGATGC TMTTTGCTG TTGCMGCCA 900 GTATTCMGC ACAAAATCAT CMCATCTTC CCCCAGTMC TCCAGAAMT GCGTTCTCCT 960 GTTGGACATC TATCAATACT ATCCTGCAAT GGGTTAATGT OGCACAACAA TTGAAATGTC 1020 GCCCCACAGG GCGTITCrGC TTTGGTCX3GG CTGGAmTA TTCAATCAAT GAAAGAGACA 1080 CCGACCTATG CXXAGTGGGA AAACGCGGCA GGCGTAnAA ΟΧΧΠΧΧΠΤ GAATTCAACA 1140 ACACjGCTAAT ACATTACAAC GCmTCTGG ATGAATCTCG CAGTGCCGCA TTAAGCACCT 1200 ACTATATCXXj TCAAGTCGCC AAGGCAGCGG 0ϋ (ΓΓΑπΑΑ AAGCCGTGAT GACTKjTATC 1260 AATACTTACT GATTGATAAT CAGGTITCrG CGGCAATAAA AACTACXXX3G ATCGCCXjAAG 1320 CTAITGCCAG TAnCAACTG TACGTCAACC GGGCATTGGA AAATGTGGAA GAAAATGCCA 1380 AITCGOjGGT TATCAGCCX3C CMTTCnTA TOjACrGGGA CAAATACAAT AAAOGCTACA 1440 GCACITGGGC (^ ΧΠΤίυΓ CAAmGTIT ACTACCdiGA AMCTATAH GATC0GACCA 1500 TGCGTATCX5G ACAAACTAAA ATGATGGACXJ CAmCTGCA ATCCGTCAGC CAAAGCTAAT 1560 TAAACGCCGA TACCGT0GAA GATGCCHTA TGTCTTATCT GACATCGTTT GAACAAGTGG 1620 CTAATCTTAA AGTTAmGC GCATATCACXJ ΑΤΑΑΤΑπΑΑ TAACGATCAA GGGCTGACTT 1680 ATTTTATCGG ACTCAGTGAA ACTGATGCCG GTGAATAm TTOCGCAGT GTCGATCACA 1740 GTAAATOAA CGACXjGTAAA ATCOTGGAG TGAATGGCAT AAAATTGATT 1800

OQO This paper size applies to Chinese National Standard (CNS) A4 size (2K) x 297 mm 509722 A7 B7 V. Description of the invention (278) GATGGCACTT 1980 GGAATACGCC AATCACCTIT GATGTCAATA AAAAAATATC CGAGCTAAAA CTGGAAAAAA 2040 ATAGAGCGCCCGGACTCTATTGTGCCGGTTATCMGGTGAAGATACGTTGCTGGTGATGT 2100 7ITATAACCA ACAAGACACA CTAGATAGTT ATAAAAACGC TTCAATGCAA GGACTATATA 2160 TCITra ^ ATATGQ: ATO secret ATATCAC_GMCAGAGCAATGTTTATCGGGATA 2220 ATAOTATCAACMTmATACCMTMTGTCAGAAGAGTGMTAACCGCTATGCAGAGG 2280 AHATGAGATTCCTTCTTCGGTMGTAGCCGTAAAGACTATGGTTGGGGAGATTATTACC 2340 TCAGCATGGT ATATMCGGA GATATTCCM CTATCMTTA CAAAGCCGCA TCMGTGAn 2400 TAAAAATTrATATITCACCAMmMGMmTTCATMTGGATATGMGGACAGMGC 2460 GCMTCMTGCMTTrGATGMTAMTATCGCAAACTAGGTGATAMTrTArrGTGTATA 2520 CCAGCTTGGG CGTTAATCCG AATAATAAGC CGAATTC 2557 ⑵ sequence identification number Information for No. 37: (i) Sequence characteristics: (A) Length: 845 amino acids (B) Type: Amino acid (C) Form: Linear (ii) Molecular type: Protein (part) (xi) Sequence description: Sequence identification number 37 ( TcdA peptide):

Ala Phe Asn lie Asp Asp Val Ser Leu Phe Arg Leu Leu Lys lie Thr 15 10 15

Asp His Asp Asn Lys Asp Gly Lys He Lys Asn Asn Leu Lys Asn Leu 20 25 30 Printed bags for employees' cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs

Ser Asn Leu Tyr lie Gly Lys Leu Leu Ala Asp He His Gin Leu Thr 35 40 45

He Asp Glu Leu Asp Leu Leu Leu lie Ala Val Gly Glu Gly Lys Thr 50 55 60

Asn Leu Ser Ala He Ser Asp Lys Gin Leu Ala Thr Leu He Arg Lys 65 70 75 80

Leu Asn Thr lie Thr Ser Trp Leu His Thr Gin Lys Trp Ser Val Phe 85 90 95 _______- The standard of this paper is applicable to China National Standard (CNS) A4 (2! 0X 297 mm) 509722 A7 B7 Employees of the Central Standards Bureau of the Ministry of Economic Affairs Printed by a consumer cooperative Lys Asp Lys Ala Asp Leu Leu His Val Met Ala Pro Tyr lie Ala 130 135 140 Ala Thr Leu Gin Leu Ser Ser Glu Asn Val Ala His Ser Val Leu Leu 145 150 155 160 Trp Ala Asp Lys Leu Gin Pro Gly Asp Gly Ala Met Thr Ala Glu Gly 165 170 175 Phe Trp Asp Trp Leu Asn Thr Lys Tyr Thr Pro Gly Ser Ser Glu Ala 180 185 190 Val Glu Thr Gin Glu His lie Val Gin Tyr Cys Gin Ala Leu Ala Gin 195 200 205 Leu Glu Met Val Tyr His Ser Thr Gly lie Asn Glu Asn Ala Phe Arg 210 215 220 Leu P he Val Thr Lys Pro Glu Met Phe Gly Ala Ala Thr Gly Ala Ala 225 230 235 240 Pro Ala His Asp Ala Leu Ser Leu lie Met Leu Thr Arg Phe Ala Asp 245 250 255 Trp Val Asn Ala Leu Gly Glu Lys Ala Ser Ser Val Leu Ala Ala Phe 260 265 270 Glu Ala Asn Ser Leu Thr Ala Glu Gin Leu Ala Asp Ala Met Asn Leu 275 280 285 Asp Ala Asn Leu Leu Leu Gin Ala Ser lie Gin Ala Gin Asn His Gin 290 295 300 His Leu Pro Val Thr Pro Glu Asn Ala Phe Ser Cys Trp Thr Ser 305 310 315 320 w.- This paper size applies to the Chinese National Standard (CNS) A4 (210X 297 mm) 509722 A7 B7 Printed by the Staff Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs Description of the invention (280) lie Asn Thr lie Leu Gin Trp Val Asn Val Ala Gin Gin Leu Lys Cys 325 330 335 Arg Pro Thr Gly Arg Phe Arg Phe Gly Arg Ala Gly Leu Tyr Ser lie 340 345 350 Asn Glu Arg Asp Thr Asp Leu Cys Pro Val Gly Lys Arg Gly Arg Arg 355 360 365 lie Asn Arg Arg Val Glu Phe Asn Asn Arg Leu lie His Tyr Asn Ala 370 375 380 Phe Leu Asp Glu Ser Arg Ser Ala Ala Ala Leu Ser Thr Tyr Tyr lie Arg 385 390 395 400 Gin Val Ala Lys Ala Ala Ala Ala lie Lys Ser Arg Asp Asp Leu Tyr 405 410 415 Gin Tyr Leu Leu lie Asp Asn Gin Val Ser Ala Ala lie Lys Thr Thr 420 425 430 Arg lie Ala Glu Ala lie Ala Ser lie Gin Leu Tyr Val Asn Arg Ala 435 440 445 Leu Glu Asn Val Glu Glu Asn Ala Asn Ser Gly Val lie Ser Arg Gin 450 455 460 Phe Phe lie Asp Trp Asp Lys Tyr Asn Lys Arg Tyr Ser Thr Trp Ala 465 470 475 480 Gly Val Ser Gin Leu Val Tyr Tyr Pro Glu Asn Tyr lie Asp Pro Thr 485 490 495 Met Arg lie Gly Gin Thr Lys Met Met Asp Ala Leu Leu Gin Ser Val 500 505 510 Ser Gin Ser Gin Leu Asn Ala Asp Thr Val Glu Asp Ala Phe Met Ser 515 520 525 Tyr Leu Thr Ser Phe Glu Gin Val Ala Asn Leu Lys Val lie Ser Ala 53 0 535 540 This paper size applies to China National Standards (CNS) A4 (210X297 mm) Please: Please read the notes on the back before filling in this page Printed on the page by the Consumer Standards Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs Description of the invention (281)

Tyr His Asp Asn lie Asn Asn Asp Gin Gly Leu Thr Tyr Phe lie Gly 545 550 555 560

Leu Ser Glu Thr Asp Ala Gly Glu Tyr Tyr Trp Arg Ser Val Asp His 565 570 575

Ser Lys Phe Asn Asp Gly Lys Phe Ala Ala Asn Ala Trp Ser Glu Trp 580 585 590

His Lys He Asp Cys Pro He Asn Pro Tyr Lys Ser Thr lie Arg Pro 595 600 605

Val lie Tyr Lys Ser Arg Leu Tyr Leu Leu Trp Leu Glu Gin Lys Glu 610 615 620

He Thr Lys Gin Thr Gly Asn Ser Lys Asp Gly Tyr Gin Thr Glu Thr 625 630 635 640

Asp Tyr Arg Tyr Glu Leu Lys Leu Ala His lie Arg Tyr Asp Gly Thr 645 650 655

Trp Asn Thr Pro lie Thr Phe Asp Val Asn Lys Lys He Ser Glu Leu 660 665 670

Lys Leu Glu Lys Asn Arg Ala Pro Gly Leu Tyr Cys Ala Gly Tyr Gin 675 680 685

Gly Glu Asp Thr Leu Leu Val Met Phe Tyr Asn Gin Gin Asp Thr Leu 690 695 700

Asp Ser Tyr Lys Asn Ala Ser Met Gin Gly Leu Tyr lie Phe Ala Asp 705 710 715 720

Met Ala Ser Lys Asp Met Thr Pro Glu Gin Ser Asn Val Tyr Arg Asp 725 730 735

Asn Ser Tyr Gin Gin Phe Asp Thr Asn Asn Val Arg Arg Val Asn Asn 740 745 750

Arg Tyr Ala Glu Asp Tyr Glu lie Pro Ser Ser Val Ser Ser Arg Lys 755 760 765 _____ This paper size applies to China National Standard (CNS) A4 size (210 X 297 mm) (Please read the precautions on the back before filling in this Page). Binding · Order 509722 A7 B7 V. Description of the invention (282)

Asp Tyr Gly Trp Gly Asp Tyr Tyr Leu Ser Met Val Tyr Asn Gly Asp 770 775 780 lie Pro Thr lie Asn Tyr Lys Ala Ala Ser Ser Asp Leu Lys He Tyr 785 790 795 800 lie Ser Pro Lys Leu Arg lie lie His Asn Gly Tyr Glu Gly Gin Lys 805 810 815

Arg Asn Gin Cys Asn Leu Met Asn Lys Tyr Gly Lys Leu Gly Asp Lys 820 825 830

Phe He Val Tyr Thr Ser Leu Gly Val Asn Pro Asn Asn 835 840 845 ⑵Sequence ID No. 38: (i) Sequence characteristics: (A) Length: 16 amino acids (B) Type: amino acid (C) Number of strands: Single strand (D) Form: Linear (ii) Molecular type: Protein (V) Fragment type: N-terminal (xi) Sequence description: Sequence identification number 38 (TcdAii-pk71 internal peptide) : Arg Tyr Tyr Asn Leu Ser Asp Glu Glu Leu Ser Gin Phe lie Gly 1 5 10 15

Information of Lys ⑵ sequence identification number 39: Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (please read the precautions on the back before filling this page) (i) Sequence characteristics: (A) Length: 20 amino groups Acid (B) Type: Amino acid (C) Number of strands: Single strand (D) Morphology: Linear (ii) Molecular type or: Protein (v) Fragment type: & -end column description: Jumeirah identification number 39 (TcdAii-pK44 internal peptide):

Gly Thr Ala Thr Asp Val Ser Gly Pro Val Glu He Asn Thr Ala _____ n This paper size applies to China National Standard (CNS) A4 (210 X 297 mm) Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 509722 A7 B7 5 、 Explanation of the invention (283) 1 5 10 15 lie Ser Pro Ala Lys 20 (2) Information of sequence identification number 40: (i) Sequence characteristics: (A) Length: 11 amino acids (B) Type: Amine Base acid (C) Number of strands: Single strand (D) Form: Linear (ii) Molecular type: Protein (v) Fragment type: N-terminal (xi) Sequence description: Sequence identification number 40 (TcbAiii N-terminal) : Ala Asn Ser Leu Thr Ala Leu Phe Leu Pro Gin 1 5 10 资料 Data of Sequence Identification Number 41: (i) Sequence characteristics: (A) Length: 14 amino acids (B) Type: amino acid ( C) Number of strands: Single strand (D) Form: Linear (ii) Molecular type: Protein (v) Fragment type: N-terminal (xi) Sequence description: Column identification number 41 (TcdAiii N-terminal): Leu Arg Ser Ala Asn Thr Leu Thr Asp Leu Phe Leu Pro Gin 1 5 10 (2) Information of sequence identification number 42: (i) Sequence characteristics: (A) Length: 19 amino acids (B) Formula: Amino acid (C) Number of strands: Single strand .. (D) Morphology: Linear (ii) Molecule i Formula: Degraded (v) Fragment type: N-terminal ---------- -· / ΛΓΙ · ^ The size of this paper is in accordance with China National Standard (CNS) A4 (210X 297mm) (Please read the precautions on the back before filling this page)

509722 A7 B7 V. Description of the invention (284) (xi) Sequence description: Sequence identification number 42 (TcdA-pk57): Arg Ala Leu Glu Val Glu Arg Thr Val Ser Leu Ala Glu Val Tyr 1 5 10 15

Ala Gly Leu Glu ⑵Sequence ID No. 43: (i) Sequence characteristics: (A) Length: 11 amino acids (B) Type: amino acids (C) Number of strands: single strand (D) morphology : Linear (ii) Molecular type: Protein (v) Fragment type: N-terminus (xi) | Column description: Julie identification number 43 (TcdAi i i-pK20 inner win): He Arg Glu Asp Tyr Pro Ala Ser Leu Gly Lys 1 5 10 资料 Data of sequence identification number 44: (i) Sequence characteristics: (A) Length: 16 amino acids (B ^ type husband: amino acids (〇1number: single stranded 0D) ) Form: Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Sexual Economy (ii) Molecular type: Protein (V) Fragment type: & (xi) sequence description at one end 'Sequence identification number 44:

Asp Asp Ser Gly Asp Asp Asp Lys Val Thr Asn Thr Asp He His Arg1 5 10 15 Standard (CNS) A4 (21 () > < 297g) 509722 A7 _____B7 V. Description of the invention (285) (A) Length: 13 amino acids (B) Type: amino acid (C) shares Number: Single strand (D) Form: Single strand (ii) Molecular type: Protein M fragment type: ri-terminal (xi) Sequence description: Sequence identification number 45:

Asp Val Xaa Gly Ser Glu Lys Ala Asn Glu Lys Leu Lys 1 5 10 (2) Information of sequence identification number 46: (i) Sequence characteristics: (A) Length: 7551 base pairs (B) Type: Nucleic acid (C) Number of strands: double strand (D) Form: linear (ii) Molecular type: DNA (gene) (xi) Sequence description: sequence identification number 46 (tcdA): ATG AAC GAG TCT GTA AAA GAG ΑΑΑ CCT GAT GTA m AAA AGC CAG TGT 48

Met Asn Glu Ser Val Lys Glu He Pro Asp Val Leu Lys Ser Gin Cys 15 10 15 GGT TIT AAT TGT CTG ACA GAT Απ AGC CAC AGC TCT TIT AAT GAA TIT 96 Gly Phe Asn Cys Leu Thr Asp He Ser His Ser Ser Phe Asn Glu Phe 20 25 30 Printed by CGC CAG CM GTA TCT GAG CAC CTC TCC TGG TCC GAA ACA CAC GAC m 144 Arg Gin Gin Val Ser Glu His Leu Ser Trp Ser Glu Thr His Asp Leu 35 40 45 TAT CAT GAT GCA CAA CAG GCA CAA AAG GAT AAT CGC CTG TAT GAA GCXJ 192 Tyr His Asp Ala Gin Gin Ala Gin Lys Asp Asn Arg Leu Tyr Glu Ala 50 55 60 (× 7Γ ATT CTC AAA CGC GCX: AAT CCC CAA m CAA AAT GCG GTG CAT CIT 240 This paper size is applicable to Chinese National Standard (CNS) A4 specification (210 X 297 mm) 509722 A7 B7 V. Description of invention (286)

Arg lie Leu Lys Arg Ala Asn Pro Gin Leu Gin Asn Ala Val His Leu 65 70 75 80 GCC ATT CTC GCT CCC MT GCT GM CTG ATA GGC TAT MC MT CM TTT 288

Ala lie Leu Ala Pro Asn Ala Glu Leu He Gly Tyr Asn Asn Gin Phe 85 90 95 AGC GGT AGA GCC AGT CM TAT GTT GCG COG GGT ACC GTT TCT TCC ATG 336

Ser Gly Arg Ala Ser Gin Tyr Val Ala Pro Gly Thr Val Ser Ser Met 100 105 110 TTC TCC CCC GCC GCT TAT TTG ACT GAA CTT TAT CXjT GAA GCA CGC AAT 384

Phe Ser Pro Ala Ala Tyr Leu Thr Glu Leu Tyr Arg Glu Ala Arg Asn 115 120 125 TTA CAC GCA AGT GAC TCC GTT TAT TAT CTG GAT ACC 0GC CX3C CCA GAT 432

Leu His Ala Ser Asp Ser Val Tyr Tyr Leu Asp Thr Arg Arg Pro Asp 130 135 140 CTC AAA TCA ATG GCG CTC AGT CAG CM AAT ATG GAT ATA GAA m TCC 480

Leu Lys Ser Met Ala Leu Ser Gin Gin Asn Met Asp He Glu Leu Ser 145 150 155 160 ACA CTX: TCT TTG TCC AAT GAG CTG TTA TTG GAA AGC Απ AAA ACT GAA 528

Thr Leu Ser Leu Ser Asn Glu Leu Leu Leu Glu Ser lie Lys Thr Glu 165 170 175 TCT AAA CTG GAA AAC TAT ACT AAA GTG ATG GAA ATG CTC TCC ACTTIX: 576

Ser Lys Leu Glu Asn Tyr Thr Lys Val Met Glu Met Leu Ser Thr Phe 180 185 190 CGT CCT TCC GCC GGC GCA ACG CCT TAT CAT GAT GCT TAT GM MT GTG GGT 624

Arg Pro Ser Gly Ala Thr Pro Tyr His Asp Ala Tyr Glu Asn Val Arg 195 200 205 GAA GTT ATC CAG CTA CM GAT CCT GGA CIT GAG CM CTC AAT GCA TCA 672

Glu Val He Gin Leu Gin Asp Pro Gly Leu Glu Gin Leu Asn Ala Ser 210 215 220 CCG GCA Απ GCC GGG TTG ATG CAT CM GCC TCC CTA TTG GGT Απ AAC 720

Pro Ala He Ala Gly Leu Met His Gin Ala Ser Leu Leu Gly He Asn 225 230 235 240 ------ 289- This paper size applies to China National Standard (CNS) A4 (210X 297 mm) 509722 A7 B7 V. Description of the invention (287) GCT TCA ATC TOG CCT GAG CTA ΤΓΤ ΑΑΤ Απ CTG kCG GAG GAG Απ ACC 768

Ala Ser lie Ser Pro Glu Leu Phe Asn He Leu Thr Glu Glu lie Thr 245 250 255 GAA GGT AAT GCT GAG GAA CIT TAT AAG AAA ΑΑΤ ΉΤ GGT AAT ATC GAA 816

Glu Gly Asn Ala Glu Glu Leu Tyr Lys Lys Asn Phe Gly Asn lie Glu 260 265 270 CCG or TCA πθ GCT ATG CCXJ GAA TAC CIT AAA CXjT TAT TAT AAT ΤΓΑ 864

Pro Ala Ser Leu Ala Met Pro Glu Tyr Leu Lys Arg Tyr Tyr Asn Leu 275 280 285 AGC GAT GM GM CIT AGT CAG TIT ATT GGT AAA GCC AGC MT ΤΓΤ GGT 912

Ser Asp Glu Glu Leu Ser Gin Phe lie Gly Lys Ala Ser Asn Phe Gly 290 295 300 CM CAG GAA TAT AGT AAT AAC CM OT Απ ACT 〇ΧΪ GTA GTC AAC AGC 960

Gin Gin Glu Tyr Ser Asn Asin Gin Leu lie Thr Pro Val Val Asn Ser 305 310 315 320 AGT GAT GGC ACG GTT AAG GTA TAT CGG ATC ACT CGC GAA TAT ACA ACC 1008

Ser Asp Gly Thr Val Lys Val Tyr Arg lie Thr Arg Glu Tyr Thr Thr 325 330 335 AAT GCT TAT CAA ATG GAT (ΠΌ GAG CTA m CCC TTC GGT GGT GAG AAT 1056

Asn Ala Tyr Gin Met Asp Val Glu Leu Phe Pro Phe Gly Gly Glu Asn 340 345 350 TAT CX3G m GAT TAT AAA TTC AAA AAT TIT TAT AAT GCC TCT TAT m 1104

Tyr Arg Leu Asp Tyr Lys Phe Lys Asn Phe Tyr Asn Ala Ser Tyr Leu 355 360 365 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling this page) TCC ATC AAG m AAT GAT AAA AGA GM OT (ΠΤ CGA ACT GM GGC GCT 1152

Ser lie Lys Leu Asn Asp Lys Arg Glu Leu Val Arg Thr Glu Gly Ala 370 375 380 CCT CAA GTC AAT ATA GAA TAC TOC GCA AAT ATC ACA ΤΓΑ AAT ACC GCT 1200

Pro Gin Val Asn lie Glu Tyr Ser Ala Asn lie Thr Leu Asn Thr Ala 385 390 395 400 GAT ΑΊχ: ACT CM CCT ΤΤΓ GAA Απ GGC CTG ACA 〇ΪΑ GTA CTT CCT TCC 1248 This paper is in accordance with the Chinese National Standard (CNS) A4 specifications (210X 2W mm) Printed by the Consumer Cooperatives of the Central Bureau of Standards of the Ministry of Economic Affairs 509722 A7 B7 V. Description of Invention (288)

Asp lie Ser Gin Pro Phe Glu lie Gly Leu Thr Arg Val Leu Pro Ser 405 410 415 GGT TCT TGG GCA TAT GCC GCC GCA AAA ΉΤ ACC GTT GM GAG TAT MC 1296

Gly Ser Trp Ala Tyr Ala Ala Ala Lys Phe Thr Val Glu Glu Tyr Asn 420 425 430 CM TAC TCT m CTG CTA AAA CTT AAC AAG GCT ΑΠ CGT CTA TCA CGT 1344

Gin Tyr Ser Phe Leu Leu Lys Leu Asn Lys Ala lie Arg Leu Ser Arg 435 440 445 GCG ACA GAA TTG TCA 00C ACG ATT CTG GM GGC ΑΠ GTG CGC ACT GTT 1392

Ala Thr Glu Leu Ser Pro Thr He Leu Glu Gly lie Val Arg Ser Val 450 455 460 AAT CTA CM CTG GAT ATC MC ACA GAC GTA m GGT AAA GTT ΉΤ CTG 1440

Asn Leu Gin Leu Asp lie Asn Thr Asp Val Leu Gly Lys Val Phe Leu 465 470 475 480 ACT AAA TAT TAT ATG CAG CGT TAT GCT ATT CAT GCT GM ACT GCC CTG 1488

Thr Lys Tyr Tyr Met Gin Arg Tyr Ala lie His Ala Glu Thr Ala Leu 485 490 495 ATA CTA TGC AAC GCXj CCT Απ TCA CAA CGT TCA TAT GAT AAT CM CCT 1536

He Leu Cys Asn Ala Pro He Ser Gin Arg Ser Tyr Asp Asn Gin Pro 500 505 510 AGC CAA TTT GAT CGC CTG ΤΓ MT MT ACG CCA TTA CTG MC GGA CM TAT 1584

Ser Gin Phe Asp Arg Leu Phe Asn Thr Pro Leu Leu Asn Gly Gin Tyr 515 520 525 ΉΤ TCT ACC GGC GAT GAG GAG Απ GAT ΓΓΑ AAT TCA GGT AGC ACC GGC 1632

Phe Ser Thr Gly Asp Glu Glu lie Asp Leu Asn Ser Gly Ser Thr Gly 530 535 540 GAT TGG OJA AAA ACC ATA CTT MG CGT GCA ΊΤΓ AAT ΚΠ GAT GAT GTC 1680

Asp Trp Arg Lys Thr lie Leu Lys Arg Ala Phe Asn He Asp Asp Val 545 550 555 560 TCG CTC TTC CGC CTG CTT AAA ATT ACC GAC CAT GAT MT AAA GAT GGA 1728

Ser Leu Phe Arg Leu Leu Lys He Thr Asp His Asp Asn Lys Asp Gly 565 570 575 This paper size applies to China National Standard (CNS) A4 size (210X297 mm) (Please read the precautions on the back before filling this page)

509722 Printed by the Consumer Cooperatives of the Central Bureau of Standards of the Ministry of Economic Affairs A7 B7 V. Invention Description (289) AAA ATT AAA AAT AAC CTA MG AAT CIT TCC AAT m TAT Απ GGA AAA 1776

Lys He Lys Asn Asn Leu Lys Asn Leu Ser Asn Leu Tyr lie Gly Lys 580 585 590 TTA CTG GCA GAT ATT CAT CM m ACC Απ GAT GM CTG GAT m m 1824

Leu Leu Ala Asp lie His Gin Leu Thr lie Asp Glu Leu Asp Leu Leu 595 600 605 CTO ATT GCX: GTA ΟΤΓ GAA GGA AAA ACT AAT m TCC GCT ATC AGT GAT 1872

Leu lie Ala Val Gly Glu Gly Lys Thr Asn Leu Ser Ala He Ser Asp 610 615 620 MG CM TTG GCT ACC CTG ATC AGA AAA CTC AAT ACT ΑΠ ACC AGC TGG 1920

Lys Gin Leu Ala Thr Leu He Arg Lys Leu Asn Thr He Thr Ser Trp 625 630 635 640 CTA CAT ACA CAG AAG TGG AGT GTA TTC CAG CTA ΉΤ ATC ATG ACT TCC 1968

Leu His Thr Gin Lys Trp Ser Val Phe Gin Leu Phe He Met Thr Ser 645 650 655 ACC AGC TAT AAC AAA AOG CTA AOG CCT GAA Απ AAG AAT TTG CTG GAT 2016

Thr Ser Tyr Asn Lys Thr Leu Thr Pro Glu He Lys Asn Leu Leu Asp 660 665 670 ACT GTC TAC CAC GGT m CAA C3GT TIT GAT AAA GAC AAA GCA GAT TTG 2064

Thr Val Tyr His Gly Leu Gin Gly Phe Asp Lys Asp Lys Ala Asp Leu 675 680 685 CTA CAT GTC ATG GCG (XC TAT ΑΠ GCG GCC ACC TTG CAA m TCA TCG 2112

Leu His Val Met Ala Pro Tyr lie Ala Ala Thr Leu Gin Leu Ser Ser 690 695 700 GAA AAT GTC GCC CAC TCG GTA CTT CTT TGG GCA GAT AAG TTA CAG CCC 2160

Glu Asn Val Ala His Ser Val Leu Leu Trp Ala Asp Lys Leu Gin Pro 705 710 715 720 GGC GAC GGC GCA ATG ACA GCA GAA AAA HC TGG GAC TGG TTG AAT ACT 2208

Gly Asp Gly Ala Met Thr Ala Glu Lys Phe Trp Asp Trp Leu Asn Thr 725 730 735 AAG TAT ACXJ CCG (3GT TCA TCG GAA GCC GTA GAA ACG CAG GAA CAT ATC 2256 -9 Q9-This paper is in accordance with the Chinese National Standard (CNS ) A4 specification (210X 297 mm) (Please read the precautions on the back before filling out this page), -ιτ 509722 A7 B7 V. Description of the invention (290)

Lys Tyr Thr Pro Gly Ser Ser Glu Ala Val Glu Thr Gin Glu His lie 740 745 750 GTT CAG TAT TGT CAG GCT CTG GCA CM TTG GAA ATG GTT TAC CAT TCC 2304

Val Gin Tyr Cys Gin Ala Leu Ala Gin Leu Glu Met Val Tyr His Ser 755 760 765 ACC GGC ATC MC GAA MC GCC TTC CGT CTA TIT GTG ACA AAA CCA GAG 2352

Thr Gly lie Asn Glu Asn Ala Phe Arg Leu Phe Val Thr Lys Pro Glu 770 775 780 ATG ΉΤ GGC GCT GCA ACT GGA GCA GCG CCC GCG CAT GAT GCC CIT TCA 2400

Met Phe Gly Ala Ala Thr Gly Ala Ala Pro Ala His Asp Ala Leu Ser 785 790 795 800 CTO Απ ATC CTC ACA CGT TIT GCG GAT ΤχΪ GTG AAC GCA CTA GGC GAA 2448

Leu lie Met Leu Thr Arg Phe Ala Asp Trp Val Asn Ala Leu Gly Glu 805 810 815 AAA GCG TCC TCG GTG CTA GCG GCA ΉΤ GM GCT AAC TCG m ACG GCA 2496

Lys Ala Ser Ser Val Leu Ala Ala Phe Glu Ala Asn Ser Leu Thr Ala 820 825 830 GM CM CTG GCT GAT GCC ATG MT CTT GAT GCT AAT TO CTG TTG CM 2544

Glu Gin Leu Ala Asp Ala Met Asn Leu Asp Ala Asn Leu Leu Leu Gin 835 840 845 GCC AGT ATT CM GCA CM MT CAT CM CAT CIT CCC CCA GTA ACT CCA 2592

Ala Ser lie Gin Ala Gin Asn His Gin His Leu Pro Pro Val Thr Pro 850 855 860 GM MT GCG TTC TCC TCC TGT TGG ACA TCT ATC MT ACT ATC CTG CAA TGG 2640 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (Please read first (Notes on the back then fill out this page)

Glu Asn Ala Phe Ser Cys Trp Thr Ser He Asn Thr lie Leu Gin Trp 865 870 875 880 GTT AAT GTC GCA CM CM TTG AAT GTC GCC CCA CAG GGC GTT TCX: GCT 2688

Val Asn Val Ala Gin Gin Leu Asn Val Ala Pro Gin Gly Val Ser Ala 885 890 895 TTG GTC GGG CTG GAT TAT ATT CM TCA ATG MA GAG ACA CCG ACC TAT 2736

Leu Val Gly Leu Asp Tyr lie Gin Ser Met Lys Glu Thr Pro Thr Tyr 900 905 910 This paper size is applicable to Chinese National Standard (CNS) A4 specification (210X ^ cent) 509722 A7 B7 V. Description of the invention (291) GCC CAG TGG GM MC GCG GCA GGC GTA m ACC GCC GGG TTG AAT TCA 2784

Ala Gin Trp Glu Asn Ala Ala Gly Val Leu Thr Ala Gly Leu Asn Ser 915 920 925 CM CAG GCT AAT ACA m CAC GCT TIT CTG GAT GM TCT CGC AGT GCC 2832

Gin Gin Ala Asn Thr Leu His Ala Phe Leu Asp Glu Ser Arg Ser Ala 930 935 940 GCA TTA AGC ACC TAC TAT ATC CGT CM GTC GCC MG GCA GCG GCG GCT 2880

Ala Leu Ser Thr Tyr Tyr lie Arg Gin Val Ala Lys Ala Ala Ala Ala 945 950 955 960 ΑΉ1 AAA AGC CX5T GAT GAC m TAT CAA TAC m CTG ΚΠ GAT AAT CAG 2928 lie Lys Ser Arg Asp Asp Leu Tyr Gin Tyr Leu Asp Asn Gin 965 970 975 GTT TCT GCG GCA ATA AAA ACC ACC CGG ATC GCC GM GCC ATT GCC AGT 2976

Val Ser Ala Ala He Lys Thr Thr Arg He Ala Glu Ala He Ala Ser 980 985 990 ATT CAA CTG TAC GTC AAC CGG GCA TTG GAA AAT GTG GAA GAA AAT GCC 3024 lie Gin Leu Tyr Val Asn Arg Ala Leu Glu Asn Val Glu Glu Asn Ala 995 1000 1005 AAT TCG GGG GTT ATC AC3C OGC CAA TO 7ΓΓ ATC GAC TGG GAC AAA TAC 3072

Asn Ser Gly Val He Ser Arg Gin Phe Phe He Asp Trp Asp Lys Tyr 1010 1015 1020 MT AAA CGC TAC AGC ACT TGG GCG GGT GIT TCT CAA TTA GTT TAC TAC 3120

Asn Lys Arg Tyr Ser Thr Trp Ala Gly Val Ser Gin Leu Val Tyr Tyr 1025 1030 1035 1040 Printed by the Staff Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (please read the notes on the back before filling this page) OXj GAA AAC TAT Απ GAT CGG ACC ATG CGT ATC GGA CAA ACC AAA ATG 3168

Pro Glu Asn Tyr He Asp Pro Thr Met Arg lie Gly Gin Thr Lys Met 1045 1050 1055 ATG GAC GCA TCA CTG CAA TCC GTC AGC CAA AGC CAA m AAC GCC GAT 3216

Met Asp Ala Leu Leu Gin Ser Val Ser Gin Ser Gin Leu Asn Ala Asp 1060 1065 1070 ACT GTC GAA GAT GCC ΤΓΤ ATG TCT TAT CTG ACA TCG TIT GAA CAA GTG 3264 This paper size applies to the Chinese National Standard (CNS) A4 specification (210X ) 509722 A7 B7 V. Description of Invention (29)

Thr Val Glu Asp Ala Phe Met Ser Tyr Leu Thr Ser Phe Glu Gin Val 1075 1080 1085 GCT MT OT AM GTT ATT AGC GCA TAT CAC GAT AAT Απ AAT AAC GAT 3312

Ala Asn Leu Lys Val He Ser Ala Tyr His Asp Asn He Asn Asn Asp 1090 1095 1100 CM GGG CTG ACC TAT TTT ATC GGA CTC AGT GAA ACT GAT GCC GGT GAA 3360

Gin Gly Leu Thr Tyr Phe He Gly Leu Ser Glu Thr Asp Ala Gly Glu 1105 1110 1115 1120 TAT TAT TGG CGC AGT GTC GAT CAC AGT AAA TO AAC GAC GGT AAA TTC 3408

Tyr Tyr Trp Arg Ser Val Asp His Ser Lys Phe Asn Asp Gly Lys Phe 1125 1130 1135 GCXj GCT MT GCC TXKj AGT GAA TGG CAT AAA ΚΠ GAT TGT CCA Απ AAC 3456

Ala Ala Asn Ala Trp Ser Glu Trp His Lys lie Asp Cys Pro He Asn 1140 1145 1150 CCT TAT AAA AGC ACT ATC CX3T CCA GTG ATA TAT AAA TCC CXjC CTG TAT 3504

Pro Tyr Lys Ser Thr lie Arg Pro Val lie Tyr Lys Ser Arg Leu Tyr 1155 1160 1165 CTG CTC TGG TTG GAA CAA AAG GAG ATC ACC AAA CAG ACA GGA AAT AGT 3552

Leu Leu Trp Leu Glu Gin Lys Glu lie Thr Lys Gin Thr Gly Asn Ser 1170 1175 1180 AAA GAT GGC TAT CAA ACT GAA ACG GAT TAT 0GT TAT GAA CTA AAA TTG 3600

Lys Asp Gly Tyr Gin Thr Glu Thr Asp Tyr Arg Tyr Glu Leu Lys Leu 1185 1190 1195 1200 GCXj CAT ATC OGC TAT GAT GGC ACT TGG AAT ACG CCA ATC A (X TIT GAT 3648 (Please read the notes on the back before filling out this page)

Ala His lie Arg Tyr Asp Gly Thr Trp Asn Thr Pro He Thr Phe Asp 1205 1210 1215 GTC AAT AAA AAA ATA TCC GAG CTA AAA CTG GAA AAA AAT AGA GOG CCC 3696

Val Asn Lys Lys lie Ser Glu Leu Lys Leu Glu Lys Asn Arg Ala Pro 1220 1225 1230 GGA CTC TAT TGT GCX: GGT TAT CAA GGT GAA GAT AOj TTG CTG GTG ATG 3744

Gly Leu Tyr Cys Ala Gly Tyr Gin Gly Glu Asp Thr Leu Leu Val Met 1235 1240 1245-, 29S: This paper size applies to China National Standard (CNS) A4 (210X297 mm) 509722 A7 B7 Employees of the Central Standards Bureau of the Ministry of Economic Affairs Printed by the Consumer Cooperatives 5. Description of the invention ΤΓΤ TAT AAC CM CM GAC ACA CTA GAT AGT TAT AAA AAC C3CT TCA ATG 3792 Phe Tyr Asn Gin Gin Asp Thr Leu Asp Ser Tyr Lys Asn Ala Ser Met 1250 1255 1260 CM GGA CTA TAT ATC ΤΓΤ GCT GAT ATG GCA TCC AAA GAT ATG ACC CCA 3840 Gin Gly Leu Tyr He Phe Ala Asp Met Ala Ser Lys Asp Met Thr Pro 1265 1270 1275 1280 GAA CAG AGC AAT GTT TAT CXjG GAT AAT AC3C TAT CM CAA TIT: 3888 Glu Gin Ser Asn Val Tyr Arg Asp Asn Ser Tyr Gin Gin Phe Asp Thr 1285 1290 1295 AAT AAT C3TC AGA AGA GTG AAT AAC CGC TAT GCA GAG GAT TAT GAG Απ 3936 Asn Asn Val Arg Arg Val Asn Asn Arg Tyr Ala Glu Asp Tyr Glu He 1300 1305 1310 CCT TCC TCG GTA AGT AGC CGT AAA GAC TAT C3GT TGG GGA GAT TAT TAC 3984 Pro Ser Ser Val Ser Ser Arg Lys Asp Tyr Gly Trp Gly Asp Tyr Tyr 1315 1 320 1325 CTC AGC ATG GTA TAT AAC GGA GAT Απ CCA ACT ATC AAT TAC AAA GCC 4032 Leu Ser Met Val Tyr Asn Gly Asp He Pro Thr lie Asn Tyr Lys Ala 1330 1335 1340 GCA TCA AGT GAT ΤΓΑ AAA ATC TAT ATC TCA CCA ΤΓΑ AGA ATT ATT 4080 Ala Ser Ser Asp Leu Lys He Tyr lie Ser Pro Lys Leu Arg He lie 1345 1350 1355 1360 CAT AAT GGA TAT GAA GGA CAG AAG OGC AAT CAA TGC AAT CTG ATG AAT 4128 His Asn Gly Tyr Glu Gly Gin Lys Arg Asn Gin Cys Asn Leu Met Asn 1365 1370 1375 AAA TAT GGC AAA CTA GGT GAT AAA TIT Απ GIT TAT ACT AGC TTG GGG 4176 Lys Tyr Gly Lys Leu Gly Asp Lys Phe He Val Tyr Thr Ser Leu Gly 1380 1385 1390 GTC AAT AAT AAC TCG TCA AAT AAG CTC ATG ΤΓΤ TAC CCC GTC TAT 4224 Val Asn Pro Asn Asn Ser Ser Asn Lys Leu Met Phe Tyr Pro Val Tyr 1395 1400 1405 CAA TAT AGC GGA AAC ACC AGT GGA CTC AAT CAA GGG AGA CTA CTA TTC 4272 (Please read the notes on the back-1 · • Items and then fill in this page),? Τ __ -jm This paper size is applicable to China National Standard (CNS) A4 size (210X 297 mm) 509722 A7 B7 5 , Invention description (29 $

Gin Tyr Ser Gly Asn Thr Ser Gly Leu Asn Gin Gly Arg Leu Leu Phe 1410 1415 1420 CAC CGT GAC ACC ACT TAT CCA TCT AAA GTA GAA GCT TGG Απ CCT GGA 4320

His Arg Asp Thr Thr Tyr Pro Ser Lys Val Glu Ala Trp lie Pro Gly 1425 1430 1435 1440 GCA AAA CGT TCT CTA ACT AAC CM AAT GCC GCC Απ GGT GAT GAT TAT 4368

Ala Lys Arg Ser Leu Thr Asn Gin Asn Ala Ala He Gly Asp Asp Tyr 1445 1450 1455 GCT ACA GAC TCT CTG AAT AAA CCXj GAT GAT CIT AAG CAA TAT ATC ΉΤ 4416

Ala Thr Asp Ser Leu Asn Lys Pro Asp Asp Leu Lys Gin Tyr He Phe 1460 1465 1470 ΑΊΌ ACT GAC AGT AAA GGG ACT GCT ACT GAT GTC TCA GGC CCA GTA GAG 4464

Met Thr Asp Ser Lys Gly Thr Ala Thr Asp Val Ser Gly Pro Val Glu 1475 1480 1485 ATT AAT ACT GCA ATT TCT CCA GCA AAA GTT CAG ΑΤΑ ATA GTC AAA GOG 4512 lie Asn Thr Ala He Ser Pro Ala Lys Val Gin lie He Val Lys Ala 1490 1495 1500 GGT GGC AAG GAG CAA ACT Ή ACC GCA GAT AAA GAT GTC TCC ΚΠ CAG 4560

Gly Gly Lys Glu Gin Thr Phe Thr Ala Asp Lys Asp Val Ser He Gin 1505 1510 1515 1520 CCA TCA CCT AGC ΤΓΤ GAT GAA ATG AAT TAT CM ΤΓΤ AAT GCC CTT GAA 4608

Pro Ser Pro Ser Phe Asp Glu Met Asn Tyr Gin Phe Asn Ala Leu Glu 1525 1530 1535 ATA GAC GGT TCT GGT CTG MT TT ATT MC MC TCA GCC AGT ATT GAT 4656 (Notes on the back then fill out this page)

He Asp Gly Ser Gly Leu Asn Phe He Asn Asn Ser Ala Ser He Asp 1540 1545 1550 GTT ACT TTT ACC GCA ΉΤ GCG GAG GAT GGC CGC AAA CTG GGT TAT GAA 4704

Val Thr Phe Thr Ala Phe Ala Glu Asp Gly Arg Lys Leu Gly Tyr Glu 1555 1560 1565 AGT TTC AGT ATT (XT GTT ACC CTC AAG GTA AGT ACC GAT AAT GCC CTG 4752

Ser Phe Ser He Pro Val Thr Leu Lys Val Ser Thr Asp Asn Ala Leu 1570 1575 1580 397_ This paper size applies to China National Standard (CNS) A4 size (210X297 mm) 509722 A7 B7 V. Description of the invention (2CT ACC CTG CAC CAT AAT GAA AAT GC3T GCG CM TAT ATG CM TGG CM TOC 4800

Thr Leu His His Asn Glu Asn Gly Ala Gin Tyr Met Gin Trp Gin Ser 1585 1590 1595 1600 TAT CGT ACC CGC CTG AAT ACT CTA TTT GCC CGC CAG TTG GTT GCA CGC 4848

Tyr Arg Thr Arg Leu Asn Thr Leu Phe Ala Arg Gin Leu Val Ala Arg 1605 1610 1615 GCC ACC ACC GGA ATC GAT ACA ATT CTG AGT ATG GAA ACT CAG MT Απ 4896

Ala Thr Thr Gly He Asp Thr He Leu Ser Met Glu Thr Gin Asn lie 1620 1625 1630 CAG GAA CCXj CAG m GGC AAA GGT TTC TAT (O AOG TTC GTG ATA CCT 4944

Gin Glu Pro Gin Leu Gly Lys Gly Phe Tyr Ala Thr Phe Val He Pro 1635 1640 1645 CCC TAT MC CTA TCA ACT CAT GGT GAT GM CGT TGG TIT MG CTT TAT 4992

Pro Tyr Asn Leu Ser Thr His Gly Asp Glu Arg Trp Phe Lys Leu Tyr 1650 1655 1660 ATC AAA CAT GTT GTT GAT AAT AAT TCA CAT Απ ATC TAT TCA GGC CAG 5040 lie Lys His Val Val Asp Asn Asn Ser His lie He Tyr Ser Gly Gin 1665 1670 1675 1680 CTA ACA GAT ACA AAT ATA AAC ATC ACA m ΊΤΓ ATT CCT CIT GAT GAT 5088

Leu Thr Asp Thr Asn lie Asn lie Thr Leu Phe lie Pro Leu Asp Asp 1685 1690 1695 GTC CCA TTG AAT CAA GAT TAT CAC GCC AAG GTT TAT ATG ACC TTC AAG 5136

Val Pro Leu Asn Gin Asp Tyr His Ala Lys Val Tyr Met Thr Phe Lys 1700 · 1705 1710 Printed by the Staff Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (please read the notes on the back before filling this page) AAA TCA CCA TCA GAT GGT ACC TGG TGG GGC CCT CAC ΉΤ GTT AGA GAT 5184

Lys Ser Pro Ser Asp Gly Thr Trp Trp Gly Pro His Phe Val Arg Asp 1715 1720 1725 GAT AAA GGA ATA GTA ACA ATA AAC CCT AAA TCC ATT TTG ACC CAT TIT 5232

Asp Lys Gly He Val Thr He Asn Pro Lys Ser He Leu Thr His Phe 1730 1735 1740 GAG AGC GTC AAT GTC CTG AAT AAT Απ AGT AGC GAA CCA ATG GAT TTC 5280 -298: — This paper size applies to Chinese National Standards (CNS) A4 specifications (210X 297 mm) 509722 A7 __ B7 V. Description of the invention (296)

Glu Ser Val Asn Val Leu Asn Asn He Ser Ser Glu Pro Met Asp Phe 1745 1750 1755 1760 AGC GGC GCT AAC AGC CTC TAT TTC TGG GM CTG TTC TAC TAT ACC COG 5328

Ser Gly Ala Asn Ser Leu Tyr Phe Trp Glu Leu Phe Tyr Tyr Thr Pro 1765 1770 1775 ATC CTG GTT GCT CM CGT TTG CTG CAT GM CAG MC TTC GAT GAA GCC 5376

Met Leu Val Ala Gin Arg Leu Leu His Glu Gin Asn Phe Asp Glu Ala 1780 1785 1790 MC OGT TGG CTG AAA TAT GTC TGG AGT CCA TGC GGT TAT Απ GTC CAC 5424

Asn Arg Trp Leu Lys Tyr Val Trp Ser Pro Ser Gly Tyr lie Val His 1795 1800 1805 GGC CAG ATT CAG MC TAC CAG TGG MC GTC OGC COG m CTG GM GAC 5472

Gly Gin He Gin Asn Tyr Gin Trp Asn Val Arg Pro Leu Leu Glu Asp 1810 1815 1820 ACC AGT TGG AAC AGT GAT CCT TTG GAT TCC GTC GAT CCT GAC GOG GTA 5520

Thr Ser Trp Asn Ser Asp Pro Leu Asp Ser Val Asp Pro Asp Ala Val 1825 1830 1835 1840 GCA CAG CAC GAT CCA ATG CAC TAC AAA GTT TCA ACT ΉΤ ATG CGT ACC 5568

Ala Gin His Asp Pro Met His Tyr Lys Val Ser Thr Phe Met Arg Thr 1845 1850 1855 TTG GAT CTA TTG ATA GCA CGC (3GC GAC CAT GCT TAT CGC CAA CTG GAA 5616

Leu Asp Leu Leu He Ala Arg Gly Asp His Ala Tyr Arg Gin Leu Glu 1860 1865 1870 OGA GAT ACA CTC AAC GAA GCG MG ATG TGG TAT ATG CAA GO; CTG CAT 5664 (Read the notes on the back and fill out this page)

Arg Asp Thr Leu Asn Glu Ala Lys Met Trp Tyr Met Gin Ala Leu His 1875 1880 1885 CTA TTA GGT GAC AAA CCT TAT CTA CCG CTG AGT ACG ACA TGG AGT GAT 5712

Leu Leu Gly Asp Lys Pro Tyr Leu Pro Leu Ser Thr Thr Trp Ser Asp 1890 1895 1900 CCA CGA CTA GAC AGA GCC GOG GAT ATC ACT ACT CAA AAT GCT CAC GAC 5760

Pro Arg Leu Asp Arg Ala Ala Asp He Thr Thr Gin Asn Ala His Asp 1905 1910 1915 1920 -9.QQ- Wood Paper Standard Applies to Chinese National Standard (CNS) A4 Specification (210X 297 mm) 509722 A7 B7 Central Standard of Ministry of Economy Printed by the Consumer Cooperative of the Bureau of the People's Republic of China 5.Invention Description (29> AGC GCA ΑΤΑ (Π € GCT CTG CXjG CAG AAT ΑΤΑ CCT ACA CCG GCA CXT m 5808 Ser Ala lie Val Ala Leu Arg Gin Asn lie Pro Thr Pro Ala Pro Leu 1925 1930 1935 TCA TTG CGC AGC GCT AAT ACC CTG ACT GAT CTC TTC CTG CCG CM ATC 5856 Ser Leu Arg Ser Ala Asn Thr Leu Thr Asp Leu Phe Leu Pro Gin lie 1940 1945 1950 AAT GAA GTG ATG ATG AAT TAC TGG CAG ACA m GCT CAG AGA GTA TAC 5904 Asn Glu Val Met Met Asn Tyr Trp Gin Thr Leu Ala Gin Arg Val Tyr 1955 1960 1965 AAT CTG CGT CAT AAC CTC TCT ATC GAC GGC CAG CCG m TAT CTG CCA 5952 Asn Leu Arg His Asn Leu Ser He Asp Gly Gin Pro Leu Tyr Leu Pro 1970 1975 1980 ATC TAT GCC ACA CCG GCC GAT CCG AAA GCG m CTC AGC GCC GCC GTT 6000 He Tyr Ala Thr Pro Ala Asp Pro Lys Ala Leu Leu Ser Ala Ala Val 1985 1990 1995 2 000 GCC ACT TCT CM GGT GGA GGC MG CTA CCG GAA TCA ΉΤ ATG TCC CTG 6048 Ala Thr Ser Gin Gly Gly Gly Gly Lys Leu Pro Glu Ser Phe Met Ser Leu 2005 2010 2015 TGG CGT TTC CCG CAC ATG CTG GAA MT GCG OGC GGC ATG GTT AGC CAG 6096 Trp Arg Phe Pro His Met Leu Glu Asn Ala Arg Gly Met Val Ser Gin 2020 2025 2030 CTC AOC CAG TTC GGC TCC ACG m CM MT Απ ATC GAA CGT CAG GAC 6144 Leu Thr Gin Phe Gly Ser Thr Leu Gin Asn He lie Glu Arg Gin Asp 2035 2040 2045 GCG GAA GCG CTC MT GCG m ΤΓΑ CM MT CAG GCC GCC GAG CTG ATA 6192 Ala Glu Ala Leu Asn Ala Leu Leu Gin Asn Gin Ala Ala Glu Leu He 2050 2055 2060 TTG ACT AAC CTG AGC Απ CAG GAC AAA ACC ATT GAA GAA TTG GAT GCC 6240 Leu Thr Asn Leu Ser He Gin Asp Lys Thr lie Glu Glu Leu Asp Ala 2065 2070 2075 2080 GAG AAA ACG GTG TTG GM MA TCC AM GOG GGA GCA CM TOG CGC TTT 6288 ( Please read the notes on the back _ 4-Items and then fill in:: Write this page) Φ ____ ^ 300 This paper size is applicable to China National Standard (CNS) A4 size (210X 297 mm) 509722 Printed by the agency A7 __B7_ 5. Description of the invention (29¾ Glu Lys Thr Val Leu Glu Lys Ser Lys Ala Gly Ala Gin Ser Arg Phe 2085 2090 2095 GAT AGC TAC GGC AAA CTG TAC GAT GAG AAT ATC AAC GCX: GGT GAA AAC 6336 Asp Ser Tyr Gly Lys Leu Tyr Asp Glu Asn lie Asn Ala Gly Glu Asn 2100 2105 2110 CM GCC ATC ACG CTA CGA GCG TCC GCC GCC GGG CTT ACC ACG GCA GTT 6384 Gin Ala Met Thr Leu Arg Ala Ser Ala Ala Gly Leu Thr Thr Ala Val 2115 2120 2125 CAG GCA TCC CGT CTG GCC GGT GCG GCG GCT GAT CTG GTG CCT AAC ATC 6432 Gin Ala Ser Arg Leu Ala Gly Ala Ala Ala Asp Leu Val Pro Asn He 2130 2135 2140 TTC GGC ΊΤΓ GCC GGT GGC GGC OGC TGG GGG GCT ATC GCT GAG GOG 6480 Phe Gly Phe Ala Gly Gly Gly Ser Arg Trp Gly Ala He Ala Glu Ala 2145 2150 2155 2160 ACA GGT TAT GTG ATC GM TTC TOC GOG AAT GTT ATG AAC ACC GAA GCG 6528 Thr Gly Tyr Val Met Glu Phe Ser Ala Asn Val Met Asn Thr Glu Ala 2165 2170 2175 GAT AAA ATT AGC CM TCT GM ACC TAC CGT CGT CGC OGT CAG GAG TGG 6576 Asp Lys He Ser Gin Ser Glu Thr Tyr Arg Arg Arg Arg Gin Glu T rp 2180 2185 2190 GAG ATC CAG CGG MT MT GO: GM GCG GAA TTG AAG CM ATC GAT GCT 6624 Glu He Gin Arg Asn Asn Ala Glu Ala Glu Leu Lys Gin He Asp Ala 2195 2200 2205 CAG CTC MA TCA CTC GCT GTA CGC GGC GM GCC GCC GTA TTG CAG AAA 6672 Gin Leu Lys Ser Leu Ala Val Arg Arg Glu Ala Ala Val Leu Gin Lys 2210 2215 2220 ACC AGT CTG AAA ACC CM CAA GAA CAG A (X CM TUT CM TTC GCC TTC 6720 Thr Ser Leu Lys Thr Gin Gin Glu Gin Thr Gin Ser Gin Leu Ala Phe 2225 2230 2235 2240 CTG CAA CGT AAG TTC AGC MT CAG GCG m TAC MC TGG CTG OGT GGT 6768 Leu Gin Arg Lys Phe Ser Asn Gin Ala Leu Tyr Asn Trp Leu Arg Gly 2245 2250 2255 (Please read the notes on the back — 0 ▼ items and then fill in-: write this page) Order m — This paper size applies to China National Standard (CNS) A4 (21 〇297297 mm) 509722 A7 B7 5. Description of the invention (㈣ CGA CTG GCG GCG Απ TAC TTC CAG TTC TAC GAT ΉΌ (KX: GTC GCG CGT 6816

Arg Leu Ala Ala lie Tyr Phe Gin Phe Tyr Asp Leu Ala Val Ala Arg 2260 2265 2270 TGC CTG ATG GCA GAA CM (ΓΓ TAC CGT TGG GAA CTC AAT GAT GAC TCT 6864

Cys Leu Met Ala Glu Gin Ala Tyr Arg Trp Glu Leu Asn Asp Asp Ser 2275 2280 2285 GCC CGC TTC ATT AM CCG GGC GCC TGG CAG GGA ACC TAT GCC GGT CTG 6912

Ala Arg Phe lie Lys Pro Gly Ala Trp Gin Gly Thr Tyr Ala Gly Leu 2290 2295 2300 CTT GCA GGT GAA ACC TTG ATG CTG AGT CTG GCA CM ATG GAA GAC GCT 6960

Leu Ala Gly Glu Thr Leu Met Leu Ser Leu Ala Gin Met Glu Asp Ala 2305 2310 2315 2320 CAT CTG AAA CXJC GAT AAA CXjC GCA m GAG GTT GAA OGC ACA GTA TCG 7008

His Leu Lys Arg Asp Lys Arg Ala Leu Glu Val Glu Arg Thr Val Ser 2325 2330 2335 CTG GCC GAA GTT TAT GCA GGA m CCA AAA GAT AAC GGT CCA TITTCX: 7056

Leu Ala Glu Val Tyr Ala Gly Leu Pro Lys Asp Asn Gly Pro Phe Ser 2340 2345 2350 CTG GCT CAG GAA ATT GAC AAG CTG GTG AC3T CM GGT TCA GGC AGT GCX: 7104

Leu Ala Gin Glu He Asp Lys Leu Val Ser Gin Gly Ser Gly Ser Ala 2355 2360 2365 GGC AGT GGT AAT MT MT TTG GCG TTC GGC GCC GGC ACG GAC ACT AAA 7152

Gly Ser Gly Asn Asn Asn Leu Ala Phe Gly Ala Gly Thr Asp Thr Lys 2370 2375 2380 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the precautions on the back before filling out this page) ACC TCTTTG CAG GCA TCA (ΠΤ TCA TTC GCT GAT TTG AAA Απ CGT GAA 7200

Thr Ser Leu Gin Ala Ser Val Ser Phe Ala Asp Leu Lys lie Arg Glu 2385 2390 2395 2400 GAT TAC CCG GCA TOG CTT GGC ΜΑ Απ CGA CGT ATC MA CAG ATC AGC 7248

Asp Tyr Pro Ala Ser Leu Gly Lys He Arg Arg lie Lys Gin lie Ser 2405 2410 2415 GTC ACT TTG CCC GCG CTA CTG GGA CCG TAT CAG GAT GTA CAG GCA ATA 7296 _-302.Γ _ This paper standard applies to Chinese national standards ( CNS) A4 specification (210 X 297 mm) 509722 A7 B7 V. Description of the invention (3 (jb

Val Thr Leu Pro Ala Leu Leu Gly Pro Tyr Gin Asp Val Gin Ala He 2420 2425 2430 TTG TCT TAC GGC GAT AAA GCC GGA m GCT MC GGC TGT GM GCG CTG 7344

Leu Ser Tyr Gly Asp Lys Ala Gly Leu Ala Asn Gly Cys Glu Ala Leu 2435 2440 2445 GCA GTT TCT CAC GGT ATG AAT GAC AGC GGC CM TTC CAG CTC GAT TTC 7392

Ala Val Ser His Gly Met Asn Asp Ser Gly Gin Phe Gin Leu Asp Phe 2450 2455 2460 MC GAT GGC AAA TTC CTG OCA TTC GM GGC ATC GCC Απ GAT CM GGC 7440

Asn Asp Gly Lys Phe Leu Pro Phe Glu Gly He Ala lie Asp Gin Gly 2465 2470 2475 2480 AOG CTG ACA CTG AGC TTC CCA AAT GCA TUT ATG (Πί GAG AM GGT AAA 7488

Thr Leu Thr Leu Ser Phe Pro Asn Ala Ser Met Pro Glu Lys Gly Lys 2485 2490 2495 CAA GCC ACT ATG m AAA ACC CTG AAC GAT ATC ΚΠ TTG CAT Απ 0GC 7536

Gin Ala Thr Met Leu Lys Thr Leu Asn Asp lie lie Leu His He Arg 2500 2505 2510 TAC ACC ATT AAA TAA 7551

Tyr Thr lie Lys *** 2516 资料 Data of sequence identification number 47: (i) Sequence characteristics: (A) Length ·. 2516 amino acids (B) Type: amino acids (C) Number of shares: single Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (D) Form: Linear (ii) Molecular Type: Protein Characteristics Start Point End Point Peptide I 2516 TcdA Protein Peptide 89 1937 TcdAii Peptide Number) Fragment 89 100 TcdAii N- End (Serial Identification Number 13 284 299 (Serial Identification Number 38)) Fragment 554 563 (Serial Identification Number 17) Fragment 1080 1092 (Serial Identification Number 23; 12/13) This paper size applies Chinese national standards (CNS) A4 specification (210X297 mm) 111—I ϋ 11ϋ I (Please read the notes on the back before filling this page) Order (xi) Sequence description: Sequence identification number 47 (TcdA): 509722 A7 B7 5 Description of the invention (301) Fragment 1385 1400 (sequence identification number 18) Fragment 1478 1497 (sequence identification number 39th) Fragment 1620 1642 (sequence identification number 21; 19/23) Fragment 1938 1948 (sequence identification number No. 41) Winning Edition 1 938 2516 TcdAiii winning fragment 2327 2345 (sequence identification number 42) fragment 2398 2408 (sequence identification number 43)

Met Asn Glu Ser Val Lys Glu lie Pro Asp Val Leu Lys Ser Gin Cys 15 10 15

Gly Phe Asn Cys Leu Thr Asp lie Ser His Ser Ser Phe Asn Glu Phe 20 25 30

Arg Gin Gin Val Ser Glu His Leu Ser Trp Ser Glu Thr His Asp Leu 35 40 45

Tyr His Asp Ala Gin Gin Ala Gin Lys Asp Asn Arg Leu Tyr Glu Ala 50 55 60

Arg He Leu Lys Arg Ala Asn Pro Gin Leu Gin Asn Ala Val His Leu 65 70 75 80

Ala He Leu Ala Pro Asn Ala Glu Leu He Gly Tyr Asn Asn Gin Phe 85 90 95

Ser Gly Arg Ala Ser Gin Tyr Val Ala Pro Gly Thr Val Ser Ser Met 100 105 110

Phe Ser Pro Ala Ala Tyr Leu Thr Glu Leu Tyr Arg Glu Ala Arg Asn 115 120 125 — I ------- install-(Please read the precautions on the back before filling this page) Set the central standard of the Ministry of Economy Printed by Bureau Consumers Cooperative

Leu His Ala Ser Asp Ser Val Tyr Tyr Leu Asp Thr Arg Arg Pro Asp 130 135 140 Leu Lys Ser Met Ala Leu Ser Gin Gin Asn Met Asp lie Glu Leu Ser 145 150 155 160 Thr Leu Ser Leu Ser Asn Glu Leu Leu Leu Glu Ser lie Lys Thr Glu 165 170 175

Ser Lys Leu Glu Asn Tyr Thr Lys Val Met Glu Met Leu Ser Thr Phe 180 185 190 -304- This paper size applies to China National Standard (CNS) A4 (210X 297 mm) 509722 A7 B7 Staff Consumption of Central Standards Bureau, Ministry of Economic Affairs Printed by the cooperative V. Description of the invention (302)

Arg Pro Ser Gly Ala Thr Pro Tyr His Asp Ala Tyr Glu Asn Val Arg 195 200 205

Glu Val lie Gin Leu Gin Asp Pro Gly Leu Glu Gin Leu Asn Ala Ser 210 215 220

Pro Ala lie Ala Gly Leu Met His Gin Ala Ser Leu Leu Gly lie Asn 225 230 235 240

Ala Ser lie Ser Pro Glu Leu Phe Asn He Leu Thr Glu Glu lie Thr 245 250 255

Glu Gly Asn Ala Glu Glu Leu Tyr Lys Lys Asn Phe Gly Asn He Glu 260 265 270

Pro Ala Ser Leu Ala Met Pro Glu Tyr Leu Lys Arg Tyr Tyr Asn Leu 275 280 285

Ser Asp Glu Glu Leu Ser Gin Phe He Gly Lys Ala Ser Asn Phe Gly 290 295 300

Gin Gin Glu Tyr Ser Asn Asn Gin Leu He Thr Pro Val Val Asn Ser 305 310 315 320

Ser Asp Gly Thr Val Lys Val Tyr Arg lie Thr Arg Glu Tyr Thr Thr 325 330 335

Asn Ala Tyr Gin Met Asp Val Glu Leu Phe Pro Phe Gly Gly Glu Asn 340 345 350

Tyr Arg Leu Asp Tyr Lys Phe Lys Asn Phe Tyr Asn Ala Ser Tyr Leu 355 360 365

Ser lie Lys Leu Asn Asp Lys Arg Glu Leu Val Arg Thr Glu Gly Ala 370 375 380

Pro Gin Val Asn He Glu Tyr Ser Ala Asn He Thr Leu Asn Thr Ala 385 390 395 400

Asp He Ser Gin Pro Phe Glu lie Gly Leu Thr Arg Val Leu Pro Ser 405 410 415 -305- This paper size applies to China National Standard (CNS) A4 (210 X 297 mm) (Please read the precautions on the back before Fill out this page)--I n —1- ~ ^ equipment · 509722 A7 B7 V. Description of the invention (3 (4

Gly Ser Trp Ala Tyr Ala Ala Ala Lys Phe Thr Val Glu Glu Tyr Asn 420 425 430

Gin Tyr Ser Phe Leu Leu Lys Leu Asn Lys Ala lie Arg Leu Ser Arg 435 440 445

Ala Thr Glu Leu Ser Pro Thr lie Leu Glu Gly lie Val Arg Ser Val 450 455 460

Asn Leu Gin Leu Asp He Asn Thr Asp Val Leu Gly Lys Val Phe Leu 465 470 475 480

Thr Lys Tyr Tyr Met Gin Arg Tyr Ala lie His Ala Glu Thr Ala Leu 485 490 495

He Leu Cys Asn Ala Pro lie Ser Gin Arg Ser Tyr Asp Asn Gin Pro 500 505 510

Ser Gin Phe Asp Arg Leu Phe Asn Thr Pro Leu Leu Asn Gly Gin Tyr 515 520 525

Phe Ser Thr Gly Asp Glu Glu lie Asp Leu Asn Ser Gly Ser Thr Gly 530 535 540

Asp Trp Arg Lys Thr lie Leu Lys Arg Ala Phe Asn lie Asp Asp Val 545 550 555 560

Ser Leu Phe Arg Leu Leu Lys lie Thr Asp His Asp Asn Lys Asp Gly 565 570 575 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling out this page)

Lys lie Lys Asn Asn Leu Lys Asn Leu Ser Asn Leu Tyr lie Gly Lys 580 585 590

Leu Leu Ala Asp lie His Gin Leu Thr lie Asp Glu Leu Asp Leu Leu 595 600 605

Leu He Ala Val Gly Glu Gly Lys Thr Asn Leu Ser Ala lie Ser Asp 610 615 620

Lys Gin Leu Ala Thr Leu He Arg Lys Leu Asn Thr He Thr Ser Trp 625 630 635 640 This paper size applies to Chinese National Standard (CNS) A4 (210X29 ^^) 509722 A7 B7 Printed by the Consumers' Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs 5. Description of the invention (㈣ Leu His Thr Gin Lys Trp Ser Val Phe Gin Leu Phe lie Met Thr Ser 645 650 655 Thr Ser Tyr Asn Lys Thr Leu Thr Pro Glu lie Lys Asn Leu Leu Asp 660 665 670 Thr Val Tyr His Gly Leu Gin Gly Phe Asp Lys Asp Lys Ala Asp Leu 675 680 685 Leu His Val Met Ala Pro Tyr lie Ala Ala Thr Leu Gin Leu Ser Ser 690 695 700 Glu Asn Val Ala His Ser Val Leu Leu Tip Ala Asp Lys Leu Gin Pro 705 710 715 720 Gly Asp Gly Ala Met Thr Ala Glu Lys Phe Trp Asp Trp Leu Asn Thr 725 730 735 Lys Tyr Thr Pro Gly Ser Ser Glu Ala Val Glu Thr Gin Glu His He 740 745 750 Val Gin Tyr Cys Gin Ala Leu Ala Gin Leu Glu Met Val Tyr His Ser 755 760 765 Thr Gly lie Asn Glu Asn Ala Phe Arg Leu Phe Val Thr Lys Pro Glu 770 775 780 Met Phe Gly Ala Ala Thr Gly Ala Ala Pro Ala His Asp Ala Leu Ser 785 790 7 95 800 Leu lie Met Leu Thr Arg Phe Ala Asp Trp Val Asn Ala Leu Gly Glu 805 810 815 Lys Ala Ser Ser Val Leu Ala Ala Phe Glu Ala Asn Ser Leu Thr Ala 820 825 830 Glu Gin Leu Ala Asp Ala Met Asn Leu Asp Ala Asn Leu Leu Leu Gin 835 840 845 Ala Ser lie Gin Ala Gin Asn His Gin His Leu Pro Pro Val Thr Pro 850 855 860 (Please read the precautions on the back before filling out this page) Installation · • Items and then fill out too much Φ This paper size applies to China National Standard (CNS) A4 (210'X 297 mm) 509722 A7 B7 V. Description of the invention

Glu Asn Ala Phe Ser Cys Trp Thr Ser He Asn Thr lie Leu Gin Trp 865 870 875 880

Val Asn Val Ala Gin Gin Leu Asn Val Ala Pro Gin Gly Val Ser Ala 885 890 895

Leu Val Gly Leu Asp Tyr He Gin Ser Met Lys Glu Thr Pro Thr Tyr 900 905 910

Ala Gin Trp Glu Asn Ala Ala Gly Val Leu Thr Ala Gly Leu Asn Ser 915 920 925

Gin Gin Ala Asn Thr Leu His Ala Phe Leu Asp Glu Ser Arg Ser Ala 930 935 940

Ala Leu Ser Thr Tyr Tyr He Arg Gin Val Ala Lys Ala Ala Ala Ala 945 950 955 960

He Lys Ser Arg Asp Asp Leu Tyr Gin Tyr Leu Leu lie Asp Asn Gin 965 970 975

Val Ser Ala Ala He Lys Thr Thr Arg He Ala Glu Ala He Ala Ser 980 985 990

He Gin Leu Tyr Val Asn Arg Ala Leu Glu Asn Val Glu Glu Asn Ala 995 1000 1005

Asn Ser Gly Val He Ser Arg Gin Phe Phe lie Asp Trp Asp Lys Tyr 1010 1015 1020

Asn Lys Arg Tyr Ser Thr Trp Ala Gly Val Ser Gin Leu Val Tyr Tyr Printed by the Staff Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling this page) 1025 1030 1035 1040

Pro Glu Asn Tyr He Asp Pro Thr Met Arg lie Gly Gin Thr Lys Met 1045 1050 1055

Met Asp Ala Leu Leu Gin Ser Val Ser Gin Ser Gin Leu Asn Ala Asp 1060 1065 1070

Thr Val Glu Asp Ala Phe Met Ser Tyr Leu Thr Ser Phe Glu Gin Val 1075 1080 1085 --J08— This paper size applies to Chinese National Standard (CNS) A4 specification (210X 297 mm) 509722 A7 B7 V. Description of the invention (30 ^

Ala Asn Leu Lys Val He Ser Ala Tyr His Asp Asn He Asn Asn Asp 1090 1095 1100

Gin Gly Leu Thr Tyr Phe lie Gly Leu Ser Glu Thr Asp Ala Gly Glu 1105 1110 1115 1120

Tyr Tyr Trp Arg Ser Val Asp His Ser Lys Phe Asn Asp Gly Lys Phe 1125 1130 1135

Ala Ala Asn Ala Trp Ser Glu Trp His Lys He Asp Cys Pro He Asn 1140 1145 1150

Pro Tyr Lys Ser Thr He Arg Pro Val He Tyr Lys Ser Arg Leu Tyr 1155 1160 1165

Leu Leu Trp Leu Glu Gin Lys Glu He Thr Lys Gin Thr Gly Asn Ser 1170 1175 1180

Lys Asp Gly Tyr Gin Thr Glu Thr Asp Tyr Arg Tyr Glu Leu Lys Leu 1185 1190 1195 1200

Ala His lie Arg Tyr Asp Gly Thr Trp Asn Thr Pro He Thr Phe Asp 1205 1210 1215

Val Asn Lys Lys He Ser Glu Leu Lys Leu Glu Lys Asn Arg Ala Pro 1220 1225 1230

Gly Leu Tyr Cys Ala Gly Tyr Gin Gly Glu Asp Thr Leu Leu Val Met 1235 1240 1245

Phe Tyr Asn Gin Gin Asp Thr Leu Asp Ser Tyr Lys Asn Ala Ser Met 1250 1255 1260

Gin Gly Leu Tyr He Phe Ala Asp Met Ala Ser Lys Asp Met Thr Pro 1265 1270 1275 1280

Glu Gin Ser Asn Val Tyr Arg Asp Asn Ser Tyr Gin Gin Phe Asp Thr 1285 1290 1295

Asn Asn Val Arg Arg Val Asn Asn Arg Tyr Ala Glu Asp Tyr Glu lie 1300 1305 1310 Private paper standards are applicable to Chinese National Standard (CNS) A4 specifications (21 〇X 297 mm) 509722 A7 B7 5. Description of the invention (3〇θ

Pro Ser Ser Val Ser Ser Arg Lys Asp Tyr Gly Trp Gly Asp Tyr Tyr 1315 1320 1325

Leu Ser Met Val Tyr Asn Gly Asp lie Pro Thr lie Asn Tyr Lys Ala 1330 1335 1340

Ala Ser Ser Asp Leu Lys He Tyr lie Ser Pro Lys Leu Arg He He 1345 1350 1355 1360

His Asn Gly Tyr Glu Gly Gin Lys Arg Asn Gin Cys Asn Leu Met Asn 1365 1370 1375

Lys Tyr Gly Lys Leu Gly Asp Lys Phe He Val Tyr Thr Ser Leu Gly 1380 1385 1390

Val Asn Pro Asn Asn Ser Ser Asn Lys Leu Met Phe Tyr Pro Val Tyr 1395 1400 1405

Gin Tyr Ser Gly Asn Thr Ser Gly Leu Asn Gin Gly Arg Leu Leu Phe 1410 1415 1420

His Arg Asp Thr Thr Tyr Pro Ser Lys Val Glu Ala Trp lie Pro Gly 1425 1430 1435 1440

Ala Lys Arg Ser Leu Thr Asn Gin Asn Ala Ala lie Gly Asp Asp Tyr 1445 1450 1455

Printed by Ala Thr Asp Ser Leu Asn Lys Pro Asp Asp Leu Lys Gin Tyr He Phe 1460 1465 1470

Met Thr Asp Ser Lys Gly Thr Ala Thr Asp Val Ser Gly Pro Val Glu 1475 1480 1485

He Asn Thr Ala He Ser Pro Ala Lys Val Gin He He Val Lys Ala 1490 1495 1500

Gly Gly Lys Glu Gin Thr Phe Thr Ala Asp Lys Asp Val Ser He Gin 1505 1510 1515 1520

Pro Ser Pro Ser Phe Asp Glu Met Asn Tyr Gin Phe Asn Ala Leu Glu 1525 1530 1535 This paper size is applicable to China National Standard (CNS) A4 specification (210X297 mm) 509722 A7 __._____ B7 V. Description of the invention (3〇8)

He Asp Gly Ser Gly Leu Asn Phe lie Asn Asn Ser Ala Ser He Asp 1540 1545 1550

Val Thr Phe Thr Ala Phe Ala Glu Asp Gly Arg Lys Leu Gly Tyr Glu 1555 1560 1565

Ser Phe Ser lie Pro Val Thr Leu Lys Val Ser Thr Asp Asn Ala Leu 1570 1575 1580

Thr Leu His His Asn Glu Asn Gly Ala Gin Tyr Met Gin Trp Gin Ser 1585 1590 1595 1600

Tyr Arg Thr Arg Leu Asn Thr Leu Phe Ala Arg Gin Leu Val Ala Arg 1605 1610 1615

Ala Thr Thr Gly He Asp Thr lie Leu Ser Met Glu Thr Gin Asn lie 1620 1625 1630

Gin Glu Pro Gin Leu Gly Lys Gly Phe Tyr Ala Thr Phe Val lie Pro 1635 1640 1645

Pro Tyr Asn Leu Ser Thr His Gly Asp Glu Arg Trp Phe Lys Leu Tyr 1650 1655 1660 lie Lys His Val Val Asp Asn Asn Ser His He lie Tyr Ser Gly Gin 1665 1670 1675 1680

Leu Thr Asp Thr Asn lie Asn lie Thr Leu Phe lie Pro Leu Asp Asp 1685 1690 1695 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economy

Val Pro Leu Asn Gin Asp Tyr His Ala Lys Val Tyr Met Thr Phe Lys 1700 1705 1710

Lys Ser Pro Ser Asp Gly Thr Trp Trp Gly Pro His Phe Val Arg Asp 1715 1720 1725

Asp Lys Gly lie Val Thr He Asn Pro Lys Ser lie Leu Thr His Phe 1730 1735 1740

Glu Ser Val Asn Val Leu Asn Asn lie Ser Ser Glu Pro Met Asp Phe 1745 1750 1755 1760 This paper size applies to China National Standard (CNS) A4 (210X297 mm) 509722 Printed by the Consumers' Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs A7 B7 V. Invention Description (309)

Ser Gly Ala Asn Ser Leu Tyr Phe Trp Glu Leu Phe Tyr Tyr Thr Pro 1765 1770 1775

Met Leu Val Ala Gin Arg Leu Leu His Glu Gin Asn Phe Asp Glu Ala 1780 1785 1790

Asn Arg Trp Leu Lys Tyr Val Trp Ser Pro Ser Gly Tyr He Val His 1795 1800 1805

Gly Gin He Gin Asn Tyr Gin Trp Asn Val Arg Pro Leu Leu Glu Asp 1810 1815 1820

Thr Ser Trp Asn Ser Asp Pro Leu Asp Ser Val Asp Pro Asp Ala Val 1825 1830 1835 1840

Ala Gin His Asp Pro Met His Tyr Lys Val Ser Thr Phe Met Arg Thr 1845 1850 1855

Leu Asp Leu Leu He Ala Arg Gly Asp His Ala Tyr Arg Gin Leu Glu 1860 1865 1870

Arg Asp Thr Leu Asn Glu Ala Lys Met Trp Tyr Met Gin Ala Leu His 1875 1880 1885

Leu Leu Gly Asp Lys Pro Tyr Leu Pro Leu Ser Thr Thr Trp Ser Asp 1890 1895 1900

Pro Arg Leu Asp Arg Ala Ala Asp lie Thr Thr Gin Asn Ala His Asp 1905 1910 1915 1920

Ser Ala He Val Ala Leu Arg Gin Asn lie Pro Thr Pro Ala Pro Leu 1925 1930 1935

Ser Leu Arg Ser Ala Asn Thr Leu Thr Asp Leu Phe Leu Pro Gin He 1940 1945 1950

Asn Glu Val Met Met Asn Tyr Trp Gin Thr Leu Ala Gin Arg Val Tyr 1955 I960 1965

Asn Leu Arg His Asn Leu Ser He Asp Gly Gin Pro Leu Tyr Leu Pro 1970 1975 1980 The paper size of the table applies to the Chinese National Standard (CNS) A4 specification (210X 297 mm) (Please read the precautions on the back before filling this page)

Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 509722 A7 B7 V. Invention Description (310)

He Tyr Ala Thr Pro Ala Asp Pro Lys Ala Leu Leu Ser Ala Ala Val 1985 1990 1995 2000

Ala Thr Ser Gin Gly Gly Gly Lys Leu Pro Glu Ser Phe Met Ser Leu 2005 2010 2015

Trp Arg Phe Pro His Met Leu Glu Asn Ala Arg Gly Met Val Ser Gin 2020 2025 2030

Leu Thr Gin Phe Gly Ser Thr Leu Gin Asn He He Glu Arg Gin Asp 2035 2040 2045

Ala Glu Ala Leu Asn Ala Leu Leu Gin Ash Gin Ala Ala Glu Leu lie 2050 2055 2060

Leu Thr Asn Leu Ser He Gin Asp Lys Thr He Glu Glu Leu Asp Ala 2065 2070 2075 2080

Glu Lys Thr Val Leu Glu Lys Ser Lys Ala Gly Ala Gin Ser Arg Phe 2085 2090 2095

Asp Ser Tyr Gly Lys Leu Tyr Asp Glu Asn lie Asn Ala Gly Glu Asn 2100 2105 2110

Gin Ala Met Thr Leu Arg Ala Ser Ala Ala Gly Leu Thr Thr Ala Val 2115 2120 2125

Gin Ala Ser Arg Leu Ala Gly Ala Ala Ala Asp Leu Val Pro Asn lie 2130 2135 2140

Phe Gly Phe Ala Gly Gly Gly Ser Arg Trp Gly Ala He Ala Glu Ala 2145 2150 2155 2160

Thr Gly Tyr Val Met Glu Phe Ser Ala Asn Val Met Asn Thr Glu Ala 2165 2170 2175

Asp Lys He Ser Gin Ser Glu Thr Tyr Arg Arg Arg Arg Gin Glu Trp 2180 2185 2190

Glu lie Gin Arg Asn Asn Ala Glu Ala Glu Leu Lys Gin lie Asp Ala 2195 2200 2205 _ This paper size applies to China National Standard (CNS) A4 specifications (210X297 mm) i I ----- II---... ..I 1---I (Please read the notes on the back before filling this page) Order 509722 A7 B7 V. Description of the invention (31) CM GCC TGC CTA TTG GGT Απ MC GCT TCA ATC TOG CCT GAG CTA TTT 480

Gin Ala Ser Leu Leu Gly lie Asn Ala Ser lie Ser Pro Glu Leu Phe 145 150 155 160 AAT Απ CTG ACXj GAG GAG Απ ACC GAA GGT AAT GCT GAG GAA CTT TAT 528

Asn lie Leu Thr Glu Glu lie Thr Glu Gly Asn Ala Glu Glu Leu Tyr 165 170 175 MG AM MT TTT GGT MT ATC GAA CCG GCC TCA TTG GCT ATG CCG GM 576

Lys Lys Asn Phe Gly Asn He Glu Pro Ala Ser Leu Ala Met Pro Glu 180 185 190 TAC CTT AAA CXjT TAT TAT AAT m AGC GAT GAA GAA CTT AGT CAG ΉΤ 624

Tyr Leu Lys Arg Tyr Tyr Asn Leu Ser Asp Glu Glu Leu Ser Gin Phe 195 200 205 Απ GGT AM (Π: AGC AAT ΤΓΤ GGT CM CAG GAA TAT AGT AAT AAC CM 672

He Gly Lys Ala Ser Asn Phe Gly Gin Gin Glu Tyr Ser Asn Asn Gin 210 215 220 CTT ATT ACT (XG GTA GTC AAC AGC AGT GAT GGC ACG GTT AAG GTA TAT 720

Leu lie Thr Pro Val Val Asn Ser Ser Asp Gly Thr Val Lys Val Tyr 225 230 235 240 CGG ATC ACC CXjC GAA TAT ACA ACC AAT GCT TAT CM ATG GAT GTG GAG 768

Arg He Thr Arg Glu Tyr Thr Thr Asn Ala Tyr Gin Met Asp Val Glu 245 250 255 CTA TIT CCT TIC GGT GGT GAG AAT TAT CGG m GAT TAT AAA TTC AAA 816

Leu Phe Pro Phe Gly Gly Glu Asn Tyr Arg Leu Asp Tyr Lys Phe Lys 260 265 270 Printed by the Staff Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (please read the notes on the back before filling this page) AAT TTT TAT AAT GCC TCT TAT TTA TCC ATC AAG m AAT GAT AAA AGA 864

Asn Phe Tyr Asn Ala Ser Tyr Leu Ser He Lys Leu Asn Asp Lys Arg 275 280 285 GM CTT GIT CGA ACT GM GGC GCT CCT CM GTC AAT ATA GM TAC TOC 912

Glu Leu Val Arg Thr Glu Gly Ala Pro Gin Val Asn He Glu Tyr Ser 290 295 300 GCA AAT ATC ACA m AAT ACC GCT GAT ATC AGT CAA CCTTIT GAA Απ 960

Ala Asn He Thr Leu Asn Thr Ala Asp He Ser Gin Pro Phe Glu He 305 310 315 320 _-314-_ This paper size applies to China National Standard (CNS) A4 size (210X297 mm) 509722 A7 B7 Central Bureau of Standards, Ministry of Economic Affairs Printing by employee consumer cooperatives V. Invention Description GTT GAA GAG TAT AAC CM TAC TCT m CTO CTA AAA CTT 1056 Lys Phe Thr Val Glu Glu Tyr Asn Gin Tyr Ser Phe Leu Leu Lys Leu 340 345 350 MC MG GCT ATT CGT CTA TCA CGT GCG ACA GAA TTG TCA CCC AOG ATT 1104 Asn Lys Ala He Arg Leu Ser Arg Ala Thr Glu Leu Ser Pro Thr lie 355 360 365 CTG GAA GGC Απ GTG OGC AGT GIT MT CTA CM CTG GAT ATC MC ACA 1152 Leu Glu Gly He Val Arg Ser Val Asn Leu Gin Leu Asp lie Asn Thr 370 375 380 GAC GTA m GGT AM GTTTTT CTG ACT AAA TAT TAT ATC CAG CGT TAT 1200 Asp Val Leu Gly Lys Val Phe Leu Thr Lys Tyr Tyr Met Gin Arg Tyr 385 390 395 400 GCT ATT CAT GCT GM ACT GCC CTG ATA CTA TGC MC GOG CCT ATT TCA 1248 Ala lie His Ala Glu Thr Ala Leu He Leu Cys Asn Ala Pro lie Ser 405 410 415 CM CGT TCA TAT GAT MT CM CCT AGC CM GAΤ GAT CGC CTG ITT AAT 1296 Gin Arg Ser Tyr Asp Asn Gin Pro Ser Gin Phe Asp Arg Leu Phe Asn 420 425 430 ACG CCA TTA CTG AAC GGA CM TAT ΉΤ TCT ACC GGC GAT GAG GAG Απ 1344 Thr Pro Leu Leu Asn Gly Gin Tyr Phe Ser Thr Gly Asp Glu Glu He 435 440 445 GAT TTA AAT TCA GGT AGC ACC GGC GAT TGG CGA AAA ACC ATA CTT AAG 1392 Asp Leu Asn Ser Gly Ser Thr Gly Asp Trp Arg Lys Thr lie Leu Lys 450 455 460 CGT GCA TTT MT Απ GAT GAT GTC TCG CTC TTC CGC CTG CTT AAA ATT 1440 Arg Ala Phe Asn He Asp Asp Val Ser Leu Phe Arg Leu Leu Lys He 465 470 475 480 ACC GAC CAT GAT AAT AAA GAT GGA AAA ATT AAA AAT AAC CTA AAG AAT 1488 Thr Asp His Asp Asn Lys Asp Gly Lys lie Lys Asn Asn Leu Lys Asn __- 315-_ This paper size applies to the Chinese National Standard (CNS) A4 specification (210X 297 mm) (Please read the notes on the back 1 #-item before filling. Write this page)

1T .1 # 509722 A7 B7 V. Description of the invention (31 > 485 490 495 CIT TOC AAT m TAT Απ GGA AAA TTA CTG GCA GAT Απ CAT CM TTA 1536

Leu Ser Asn Leu Tyr lie Gly Lys Leu Leu Ala Asp lie His Gin Leu 500 505 510 ACC ATT GAT GAA CTG GAT m m CTG ATT GCC GTA GGT GAA GGA AAA 1584

Thr lie Asp Glu Leu Asp Leu Leu Leu lie Ala Val Gly Glu Gly Lys 515 520 525 ACT MT m TCC GCT ATC AGT GAT MG CM TTG GCT ACC CTG ATC AGA 1632

Thr Asn Leu Ser Ala lie Ser Asp Lys Gin Leu Ala Thr Leu He Arg 530 535 540 AAA CTC AAT ACT ATT AOC AGC TGG CTA CAT ACA CAG AAG TGG AGT GTA 1680

Lys Leu Asn Thr He Thr Ser Trp Leu His Thr Gin Lys Trp Ser Val 545 550 555 560 TTC CAG CTA ΉΤ ATC ATG ACC TCC ACC AGC TAT AAC AAA AOG CTA ACXJ 1728

Phe Gin Leu Phe lie Met Thr Ser Thr Ser Tyr Asn Lys Thr Leu Thr 565 570 575 CCT GAA Απ AAG AAT TTG CTG GAT ACC GTC TAC CAC GGT m CAA GGT 1776

Pro Glu He Lys Asn Leu Leu Asp Thr Val Tyr His Gly Leu Gin Gly 580 585 590 7ΓΓ GAT AAA GAC AAA GCA GAT TTG CTA CAT GTC ATG GCG CCC TAT ATT 1824

Phe Asp Lys Asp Lys Ala Asp Leu Leu His Val Met Ala Pro Tyr He 595 600 605 GCG GCX: ACC TTG CAA TTA TCA TGG GAA AAT GTC GCC CAC TOG GTA CTC 1872

Ala Ala Thr Leu Gin Leu Ser Ser Glu Asn Val Ala His Ser Val Leu Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling out this page) 610 615 620 CTT TGG GCA GAT AAG m CAG CCC GGC GAC GC5C GCA ATG ACA GCA GAA 1920

Leu Trp Ala Asp Lys Leu Gin Pro Gly Asp Gly Ala Met Thr Ala Glu 625 630 635 640 AAA TTC TGG GAC TGG TTG AAT ACT MG TAT ACG CCG GGT TCA TOG GAA 1968

Lys Phe Trp Asp Trp Leu Asn Thr Lys Tyr Thr Pro Gly Ser Ser Glu 645 650 655 GCC GTA GAA ACG CAG GAA CAT ATC (7ΓΤ CAG TAT TGT CAG GCT CTG GCA 2016 _ -316-_ This paper standard applies to Chinese national standards ( CNS) A4 specification (210X297 mm ^ 509722 A7 B7 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 5. Description of the invention (31 > Ala Val Glu Thr Gin Glu His He Val Gin Tyr Cys Gin Ala Leu Ala 660 665 670 CM TTC GAA ATG GTT TAC CAT TCC ACC GGC ATC AAC GAA AAC GCC TTC 2064 Gin Leu Glu Met Val Tyr His Ser Thr Gly lie Asn Glu Asn Ala Phe 675 680 685 CGT CTA TIT GTG ACA AAA CCA GAG ATG TIT GGC C3CT GCA ACT GGA 2112 Arg Leu Phe Val Thr Lys Pro Glu Met Phe Gly Ala Ala Thr Gly Ala 690 695 700 GCG CCC GCG CAT GAT GCC CTT TCA CTG ATT ATG CFG ACA OGT TIT GCG 2160 Ala Pro Ala His Asp Ala Leu Ser Leu He Met Leu Thr Arg Phe Ala 705 710 715 720 GAT TGG GTG MC GCA CTA GGC GAA AAA GCG TGC TOG GTG CTA GOG GCA 2208 Asp Trp Val Asn Ala Leu Gly Glu Lys Ala Ser Ser Val Leu Ala Ala 725 730 735 ΤΓΤ GAA GCT AAC TCG m A0G GCA GAA CM CTG GCT GAT GCC ATG AAT 2256 Phe Glu Ala Asn Ser Leu Thr Ala Glu Gin Leu Ala Asp Ala Met Asn 740 745 750 CTT GAT GCT MT TTG CTG TTG CM GCC AGT ATT CM GCA CM AAT CAT 2304 Le Asp Ala Asn Leu Leu Leu Gin Ala Ser He Gin Ala Gin Asn His 755 760 765 CM CAT CTT CCC CCA GTA ACT CCA GM MT GCG TTC TCC TGT TGG ACA 2352 Gin His Leu Pro Pro Val Thr Pro Glu Asn Ala Phe Ser Cys Trp Thr 770 775 780 TCT ATC AAT ACT ATC CTG CAA TGG GTT AAT GTC GCA CAA CAA TTG AAT 2400 Ser lie Asn Thr lie Leu Gin Trp Val Asn Val Ala Gin Gin Leu Asn 785 790 795 800 GTC GCC CCA CAG GGC GTT TCC GCT TTG GTC GGG CTG GAT TAT ATT CM 2448 Val Ala Pro Gin Gly Val Ser Ala Leu Val Gly Leu Asp Tyr He Gin 805 810 815 TCA ATG AAA GAG ACA CCG ACC TAT GCC CAG TGG GM MC GCG GCA GGC 2496 Ser Met Lys Glu Thr Pro Thr Tyr Ala Gin Trp Glu Asn Ala Ala Gly 820 825 830 (Please read the notes on the back _ 1 · Items and fill in.: Write this page) Order _-317- This paper size applies to China National Standard (CNS) A4 specifications (210X 297 mm) 509722 A7 B7 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economy 835 840 845 ΉΤ CTG GAT GM TCT CGC AGT GCC GCA TTA AGC ACC TAC TAT ATC CGT 2592 Phe Leu Asp Glu Ser Arg Ser Ala Ala Leu Ser Thr Tyr Tyr lie Arg 850 855 860 CM GTC GCC MG GCA GCG GCG GCT Απ AAA CGT GAT GAC TTG TAT 2640 Gin Val Ala Lys Ala Ala Ala Ala He Lys Ser Arg Asp Asp Leu Tyr 865 870 875 880 CM TAC m CTG Απ GAT MT CAG GTT TCT GCG GCA ATA AAA ACC ACC 2688 Gin Tyr Leu Leu He Asp Asn Gin Val Ser Ala Ala lie Lys Thr Thr 885 890 895 CGG ATC GCC GM GCC Απ GCC AGT ATT CM CTG TAC GTC MC CGG GCA 2736 Arg He Ala Glu Ala He Ala Ser He Gin Leu Tyr Val Asn Arg Ala 900 905 910 TTG GM MT GTG GM GM MT GCC MT TOG GGG GTT ATC AGC OGC CM 2784 Leu Glu Asn Val Glu Glu Asn Ala Asn Ser Gly Val He Ser Arg Gin 915 920 925 TTC ΉΤ ATC GAC TGG GAC AAA TAC AAT AAA CGC TAC AGC ACT TGG GOj 2832 Phe Phe He Asp Trp Asp Lys Tyr Asn Lys Arg Tyr Ser Thr Trp Ala 930 935 940 GCT GIT TCT CAA m GIT TAC TAC CCXj GM AAC TAT Απ GAT CCG ACC 2880 Gly Val Ser Gin Leu Val Tyr Tyr Pro Glu Asn Tyr He Asp Pro Thr .945 950 955 960 ATG CGT ATC GGA CAA ACC AAA ATG ATG GAC GCA m CTG CM TCC GTC 2928 Met Arg lie Gly Gin Thr Lys Met Met Asp Ala Leu Leu Gin Ser Val 965 970 975 AGC CAA AGC CAA m AAC GCC GAT ACC GTC GAA GAT GCC TIT ATG TCT 2976 Ser Gin Ser Gin Leu Asn Ala Asp Thr Val Glu Asp Ala Phe Met Ser 980 985 990 TAT CTG ACA TCG TIT GM CAA GTG GCT AAT CTT AAA GTT ATT AGC GCA 3024 Tyr Leu Thr Ser Phe Glu Gin Val Ala Asn Leu Lys Val lie Ser Ala 995 1000 1005 __ This paper size applies to the Chinese National Standard (CNS) A4 size (21 OX 297 mm) (Please read the precautions on the back to install II-item before filling this Page) Ordered Φ 509722 A7 B7 V. Description of Invention (TAT CAC GAT AAT Απ AAT AAC GAT CAA GGG CTG A (X TAT TTT ATC GGA 3072

Tyr His Asp Asn lie Asn Asn Asp Gin Gly Leu Thr Tyr Phe lie Gly 1010 1015 1020 (Please read the notes on the back before filling out this page) CTC AGT GAA ACT GAT GCC GGT GAA TAT TAT TGG CGC AGT GTC GAT CAC 3120

Leu Ser Glu Thr Asp Ala Gly Glu Tyr Tyr Trp Arg Ser Val Asp His 1025 1030 1035 1040 AGT MA TTC MC GAC GGT AM TTC GOG GCT MT GCC TGG AGT GAA TGG 3168

Ser Lys Phe Asn Asp Gly Lys Phe Ala Ala Asn Ala Trp Ser Glu Trp 1045 1050 1055 CAT AAA ATT GAT TGT CCA Απ AAC CCT TAT AAA AGC ACT ATC CGT CCk 3216

His Lys He Asp Cys Pro lie Asn Pro Tyr Lys Ser Thr He Arg Pro 1060 1065 1070 GTG ATA TAT MA TCC CGC CTG TAT CTG CTC TGG TTG GM CM MG GAG 3264

Val lie Tyr Lys Ser Arg Leu Tyr Leu Leu Trp Leu Glu Gin Lys Glu 1075 1080 1085 ATC ACC AAA CAG ACA C3GA AAT AGT AAA GAT GGC TAT CAA ACT GAA AOG 3312 lie Thr Lys Gin Thr Gly Asn Ser Lys Asp Gly Tyr Gin Tin Glu Thr 1090 1095 1100 GAT TAT OGT TAT GAA CTA AAA TKJ GCG CAT ATC CX3C TAT GAT GGC ACT 3360

Asp Tyr Arg Tyr Glu Leu Lys Leu Ala His lie Arg Tyr Asp Gly Thr 1105 1110 1115 1120 TGG AAT AOG CCA ATC ACC Ή GAT GTC AAT AAA AAA ATA TCC GAG CTA 3408

Trp Asn Thr Pro He Thr Phe Asp Val Asn Lys He Ser Glu Leu 1125 1130 1135 AM CTG GAA AAA AAT AGA GCG CCC GGA CTC TAT TGT GCC GGT TAT CAA 3456

Lys Leu Glu Lys Asn Arg Ala Pro Gly Leu Tyr Cys Ala Gly Tyr Gin 1140 1145 1150 GGT GM GAT AOG TTG CTG GTG ATG TTT TAT MC CM CM GAC ACA CTA 3504

Gly Glu Asp Thr Leu Leu Val Met Phe Tyr Asn Gin Gin Asp Thr Leu 1155 1160 1165 GAT AC5T TAT AAA AAC GCT TCA ATG CAA GGA CTA TAT ATC TTT GCT GAT 3552

Asp Ser Tyr Lys Asn Ala Ser Met Gin Gly Leu Tyr He Phe Ala Asp _-319-_ This paper size applies to Chinese National Standard (CNS) A4 specification (210X 297 mm) 509722 A7 B7 V. Description of the invention (31 > 1170 1175 1180 ATG GCA TCC AAA GAT ATG ACC CCA GAA CAG AGC AAT GTT TAT CGG GAT 3600

Met Ala Ser Lys Asp Met Thr Pro Glu Gin Ser Asn Val Tyr Arg Asp 1185 1190 1195 1200 AAT AGC TAT CM CM ΤΓΤ GAT ACC AAT AAT GTC AGA AGA GTG AAT AAC 3648

Asn Ser Tyr Gin Gin Phe Asp Thr Asn Asn Val Arg Arg Val Asn Asn 1205 1210 1215 CGC TAT GCA GAG GAT TAT GAG Απ CCT TCC TOG GTA AGT AGC CGT AAA 3696

Arg Tyr Ala Glu Asp Tyr Glu lie Pro Ser Ser Val Ser Ser Arg Lys 1220 1225 1230 GAC TAT GGT TGG GGA GAT TAT TAC CTC AGC ATG GTA TAT AAC GGA GAT 3744

Asp Tyr Gly Trp Gly Asp Tyr Tyr Leu Ser Met Val Tyr Asn Gly Asp 1235 1240 1245 Απ CCk ACT ATC AAT TAC AAA GCC GCA TCA AGT GAT m AAA ATC TAT 3792

He Pro Thr lie Asn Tyr Lys Ala Ala Ser Ser Asp Leu Lys He Tyr 1250 1255 1260 ATC TCA CCA AAA ΊΓΓΑ AGA Απ Απ CAT AAT GGA TAT GAA GGA CAG AAG 3840

He Ser Pro Lys Leu Arg He lie His Asn Gly Tyr Glu Gly Gin Lys 1265 1270 1275 1280 ΟΓ MT CM TGC AAT CTG ATG AAT AAA TAT GGC AAA CTA GGT GAT AAA 3888

Arg Asn Gin Cys Asn Leu Met Asn Lys Tyr Gly Lys Leu Gly Asp Lys 1285 1290 1295 ΓΓ ATT GTT TAT ACT AGC TTG GGG GTC AAT CCA AAT AAC TCXj TCA AAT 3936

Phe He Val Tyr Thr Ser Leu Gly Val Asn Pro Asn Asn Ser Ser Asn Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (please read the notes on the back before filling out this page) 1300 1305 1310 AAG CTC ATG TG TAC CCC GTC TAT CM TAT AGC GGA AAC ACC AGT GGA 3984

Lys Leu Met Phe Tyr Pro Val Tyr Gin Tyr Ser Gly Asn Thr Ser Gly 1315 1320 1325 CTC AAT CM GGG AGA CTA CTA TTC CAC OGT GAC ACC ACT TAT CCA TCT 4032

Leu Asn Gin Gly Arg Leu Leu Phe His Arg Asp Thr Thr Tyr Pro Ser 1330 1335 1340 AM GTA GAA GCT TGG Απ CCT GGA GCA AAA CGT TCT CTA ACC AAC CM 4080 __- 320 · _ This paper standard applies to Chinese national standards (CNS ) A4 specification (210X 297 mm) 509722 A7 B7 V. Description of invention (d

Lys Val Glu Ala Trp lie Pro Gly Ala Lys Arg Ser Leu Thr Asn Gin 1345 1350 1355 1360 AAT GCC GCC ATT GGT GAT GAT TAT GCT ACA GAC TCT CTG AAT AAA COG 4128 (Please read the notes on the back before filling this page)

Asn Ala Ala lie Gly Asp Asp Tyr Ala Thr Asp Ser Leu Asn Lys Pro 1365 1370 1375 GAT GAT CTT AAG CM TAT ATC TIT ΑΊΌ ACT GAC AGT AAA GGG ACT GCT 4176

Asp Asp Leu Lys Gin Tyr lie Phe Met Thr Asp Ser Lys Gly Thr Ala 1380 1385 1390 ACT GAT GTC TCA GGC CCA GTA GAG ATT AAT ACT GCA Απ TCT CCA GCA 4224

Thr Asp Val Ser Gly Pro Val Glu He Asn Thr Ala He Ser Pro Ala 1395 1400 1405 AAA GTT CAG ΑΤΑ ATA GTC AAA GCG GGT GGC AAG GAG CM ACT ΉΤ AOC 4272

Lys Val Gin lie He Val Lys Ala Gly Gly Lys Glu Gin Thr Phe Thr 1410 1415 1420 GCA GAT AAA GAT GTC TCC Απ CAG OCA TCA CCT AC3C TIT GAT GAA ATG 4320

Ala Asp Lys Asp Val Ser lie Gin Pro Ser Pro Ser Phe Asp Glu Met 1425 1430 1435 1440 AAT TAT CM TIT AAT GCC CTT GAA ATA GAC GGT TUT GCT CTG AAT ΉΤ 4368

Asn Tyr Gin Phe Asn Ala Leu Glu lie Asp Gly Ser Gly Leu Asn Phe 1445 1450 1455 ATT MC MC TCA GCC AGT Απ GAT GTT ACT TTT ACC GCA ΉΤ GCG GAG 4416

He Asn Asn Ser Ala Ser He Asp Val Thr Phe Thr Ala Phe Ala Glu 1460 1465 1470 GAT GGC CXT AAA CTG GGT TAT GAA AGT TO ACT Απ (ΓΓ GTT ACC CTC 4464 Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs

Asp Gly Arg Lys Leu Gly Tyr Glu Ser Phe Ser lie Pro Val Thr Leu 1475 1480 1485 AAG GTA AGT ACC GAT MT GCT CTG ACC CTG CAC CAT AAT GAA AAT GGT 4512

Lys Val Ser Thr Asp Asn Ala Leu Thr Leu His His Asn Glu Asn Gly 1490 1495 1500 GOG CM TAT ΑΊΌ CM TGG CAA TCC TAT (OT A (X CGC CTG AAT ACT CTA 4560

Ala Gin Tyr Met Gin Trp Gin Ser Tyr Arg Thr Arg Leu Asn Thr Leu 1505 1510 1515 1520 _ -321- This paper size applies to China National Standard (CNS) A4 specifications (210X 297 mm 509722 A7 B7 V. Description of the invention ( 3li ΉΤ GCC CGC CAG TTG GIT GCA CGC GCC ACC ACC GGA ATC GAT ACA ATT 4608

Phe Ala Arg Gin Leu Val Ala Arg Ala Thr Thr Gly lie Asp Thr He 1525 1530 1535 CTG AGT ATG GAA ACT CAG AAT Απ CAG GAA CCG CAG ΊΤΑ GGC AAA GGT 4656

Leu Ser Met Glu Thr Gin Asn lie Gin Glu Pro Gin Leu Gly Lys Gly 1540 1545 1550 TTC TAT (KT ACXj TTC GTG ATA CCT CCC TAT AAC CTA TCA ACT CAT GGT 4704

Phe Tyr Ala Thr Phe Val lie Pro Pro Tyr Asn Leu Ser Thr His Gly 1555 1560 1565 GAT GM CGT TGG GG MG CTT TAT ATC AAA CAT GTT GTT GAT AAT AAT 4752

Asp Glu Arg Trp Phe Lys Leu Tyr He Lys His Val Val Asp Asn Asn 1570 1575 1580 TCA CAT Απ ATC TAT TCA GGC CAG CTA ACA GAT ACA AAT ATA AAC ATC 4800

Ser His lie He Tyr Ser Gly Gin Leu Thr Asp Thr Asn He Asn lie 1585 1590 1595 1600 ACA ΊΤΑ ΊΤΓ ATT CCT CIT GAT GAT GTC CCA TTG AAT CM GAT TAT CAC 4848

Thr Leu Phe He Pro Leu Asp Asp Val Pro Leu Asn Gin Asp Tyr His 1605 1610 1615 GCC AAG GTT TAT ATG ACC WC AAG AAA TCA CCA TCA GAT GGT ACC TGG 4896

Ala Lys Val Tyr Met Thr Phe Lys Lys Ser Pro Ser Asp Gly Thr Trp 1620 1625 1630 TGG GGC CCT CAC ΉΤ GTT AGA GAT GAT AAA GGA ATA GTA ACA ATA MC 4944

Trp Gly Pro His Phe Val Arg Asp Asp Lys Gly He Val Thr He Asn 1635 1640 1645 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the precautions on the back before filling this page) CCT AAA TCT ATTTKj ACC CAT TTT GAG AGC GTC AAT GTC CTG AAT AAT 4992

Pro Lys Ser lie Leu Thr His Phe Glu Ser Val Asn Val Leu Asn Asn 1650 1655 1660 ATT AGT AGC GAA CCA ATG GAT TTC AGC GGC GCT AAC AGC CTC TAT TTC 5040

He Ser Ser Glu Pro Met Asp Phe Ser Gly Ala Asn Ser Leu Tyr Phe 1665 1670 1675 1680 TGG GM CTG TTC TAC TAT ACC CCG ATG CTG GTT GCT CM CGT TTG CTG 5088

Trp Glu Leu Phe Tyr Tyr Thr Pro Met Leu Val Ala Gin Arg Leu Leu 1685 1690 1695 _-322-_ This paper size applies to China National Standard (CNS) A4 specifications (210X 297 mm) 509722 A7 B7 V. Description of the invention ( 32)) CAT GAA CAG MC TK: GAT GM (Π: MC CGT ΤΚΚ CTG AAA TAT GTC TCG 5136

His Glu Gin Asn Phe Asp Glu Ala Asn Arg Trp Leu Lys Tyr Val Trp 1700 1705 1710 AGT CCA TCC GGT TAT Απ GTC CAC GGC CAG ATT CAG AAC TAC CAG TGG 5184

Ser Pro Ser Gly Tyr lie Val His Gly Gin lie Gin Asn Tyr Gin Trp 1715 1720 1725 MC GTC CGC COG ΤΓΑ CTG GAA GAC ACC AC3T TGG AAC AGT GAT CCT TTG 5232

Asn Val Arg Pro Leu Leu Glu Asp Thr Ser Trp Asn Ser Asp Pro Leu 1730 1735 1740 GAT TCC GTC GAT CCT GAC GCG GTA GCA CAG CAC GAT OCA ATG CAC TAC 5280

Asp Ser Val Asp Pro Asp Ala Val Ala Gin His Asp Pro Met His Tyr 1745 1750 1755 1760 AAA GTT TCA ACT ΉΤ ATG CGT ACT TTG GAT CTA TTG ATA GCA OGC C3GC 5328

Lys Val Ser Thr Phe Met Arg Thr Leu Asp Leu Leu He Ala Arg Gly 1765 1770 1775 GAC CAT GCT TAT CGC CAA CTG GAA CGA GAT ACA CTC AAC GAA GCG AAG 5376

Asp His Ala Tyr Arg Gin Leu Glu Arg Asp Thr Leu Asn Glu Ala Lys 1780 1785 1790 ATG TGG TAT ATG CAA GCG CTG CAT CTA m GGT GAC AAA (XT TAT CTA 5424

Met Trp Tyr Met Gin Ala Leu His Leu Leu Gly Asp Lys Pro Tyr Leu 1795 1800 1805 CCG CTG AGT ACG ACA TGG AGT GAT CCA CGA CTA GAC AGA GCC GCXj GAT 5472

Pro Leu Ser Thr Thr Trp Ser Asp Pro Arg Leu Asp Arg Ala Ala Asp 1810 1815 1820 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling this page) ATC ACT ACC CM MT GCT CAC GAC AGC GCA ATA GTC GCT CTG CGG CAG 5520 lie Thr Thr Gin Asn Ala His Asp Ser Ala lie Val Ala Leu Arg Gin 1825 1830 1835 1840 AAT ATA CCT ACA CCG GCA CCT m TCA 5547

Asn lie Pro Thr Pro Ala Pro Leu Ser 1845 1849 资料 Serial Identification Number No. 49: (i) Sequence characteristics: _ -323-_ This paper size applies Chinese National Standard (CNS) A4 specification (210X297 mm) 509722 A7 B7 5. Description of the invention (32 ^ (A) Length: 1849 amino acids (B) Type: Amino acid (C) Number of strands: Single strand (D) Form: Linear (ii) Molecular type: Protein ) Sequence description: Sequence identification number 49 (TcdAii): Characterization of origin, endpoint description, peptide 1 1849 ) Fragment 466 475 (sequence identification number remaining 17) fragment 993 1004 (sequence identification number 23; 12Λ3) fragment 1297 1312 (sequence identification number 18) fragment 1390 1409 (sequence identification number 39; S fragment I532 I554 (Serial ID No. 21; 19/23) Leu lie Gly Tyr Asn Asn Gin Phe Ser Gly Arg Ala Ser Gin Tyr Val 1 5 10 15

Ala Pro Gly Thr Val Ser Ser Met Phe Ser Pro Ala Ala Tyr Leu Thr 20 25 30 Glu Leu Tyr Arg Glu Ala Arg Asn Leu His Ala Ser Asp Ser Val Tyr 35 40 45 Tyr Leu Asp Thr Arg Arg Pro Asp Leu Lys Ser Met Ala Leu Ser Gin 50 55 60 (Please read the notes on the back before filling out this page) * 装-

Printed by 1T Shellfish Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs

Gin Asn Met Asp lie Glu Leu Ser Thr Leu Ser Leu Ser Asn Glu Leu 65 70 75 80

Leu Leu Glu Ser lie Lys Thr Glu Ser Lys Leu Glu Asn Tyr Thr Lys 85 90 95

Val Met Glu Met Leu Ser Thr Phe Arg Pro Ser Gly Ala Thr Pro Tyr 100 105 110 His Asp Ala Tyr Glu Asn Val Arg Glu Val He Gin Leu Gin Asp Pro 115 120 125 _ -324- This paper size applies to Chinese national standards ( CNS) A4 specification (210X297 mm) 509722 A7 B7 V. Description of invention (3 must

Gly Leu Glu Gin Leu Asn Ala Ser Pro Ala lie Ala Gly Leu Met His 130 135 140

Gin Ala Ser Leu Leu Gly He Asn Ala Ser lie Ser Pro Glu Leu Phe 145 150 155 160

Asn He Leu Thr Glu Glu lie Thr Glu Gly Asn Ala Glu Glu Leu Tyr 165 170 175

Lys Lys Asn Phe Gly Asn He Glu Pro Ala Ser Leu Ala Met Pro Glu 180 185 190

Tyr Leu Lys Arg Tyr Tyr Asn Leu Ser Asp Glu Glu Leu Ser Gin Phe 195 200 205 lie Gly Lys Ala Ser Asn Phe Gly Gin Gin Glu Tyr Ser Asn Asn Gin 210 215 220

Leu lie Thr Pro Val Val Asn Ser Ser Asp Gly Thr Val Lys Val Tyr 225 230 235 240

Arg lie Thr Arg Glu Tyr Thr Thr Asn Ala Tyr Gin Met Asp Val Glu 245 250 255

Leu Phe Pro Phe Gly Gly Glu Asn Tyr Arg Leu Asp Tyr Lys Phe Lys 260 265 270

Asn Phe Tyr Asn Ala Ser Tyr Leu Ser lie Lys Leu Asn Asp Lys Arg 275 280 285

Glu Leu Val Arg Thr Glu Gly Ala Pro Gin Val Asn He Glu Tyr Ser 290 295 300 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling out this page)

Ala Asn He Thr Leu Asn Thr Ala Asp He Ser Gin Pro Phe Glu He 305 310 315 320

Gly Leu Thr Arg Val Leu Pro Ser Gly Ser Trp Ala Tyr Ala Ala Ala 325 330 335

Lys Phe Thr Val Glu Glu Tyr Asn Gin Tyr Ser Phe Leu Leu Lys Leu 340 345 350

Asn Lys Ala He Arg Leu Ser Arg Ala Thr Glu Leu Ser Pro Thr lie __- 325-_ This paper size applies to China National Standard (CNS) A4 specification (210X297 mm) 509722 A7 B7 V. Description of the invention (34 355 360 365

Leu Glu Gly lie Val Arg Ser Val Asn Leu Gin Leu Asp lie Asn Thr 370 375 380

Asp Val Leu Gly Lys Val Phe Leu Thr Lys Tyr Tyr Met Gin Arg Tyr 385 390 395 400

Ala He His Ala Glu Thr Ala Leu He Leu Cys Asn Ala Pro He Ser 405 410 415

Gin Arg Ser Tyr Asp Asn Gin Pro Ser Gin Phe Asp Arg Leu Phe Asn 420 425 430

Thr Pro Leu Leu Asn Gly Gin Tyr Phe Ser Thr Gly Asp Glu Glu lie 435 440 445

Asp Leu Asn Ser Gly Ser Thr Gly Asp Trp Arg Lys Thr lie Leu Lys 450 455 460

Arg Ala Phe Asn He Asp Asp Val Ser Leu Phe Arg Leu Leu Lys lie 465 470 475 480

Thr Asp His Asp Asn Lys Asp Gly Lys He Lys Asn Asn Leu Lys Asn ’485 490 495

Leu Ser Asn Leu Tyr He Gly Lys Leu Leu Ala Asp He His Gin Leu 500 505 510

Thr lie Asp Glu Leu Asp Leu Leu Leu lie Ala Val Gly Glu Gly Lys 515 520 525 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling out this page)

Thr Asn Leu Ser Ala lie Ser Asp Lys Gin Leu Ala Thr Leu lie Arg 530 535 540

Lys Leu Asn Thr lie Thr Ser Trp Leu His Thr Gin Lys Trp Ser Val 545 550 555 560

Phe Gin Leu Phe He Met Thr Ser Thr Ser Tyr Asn Lys Thr Leu Thr 565 570 575

Pro Glu lie Lys Asn Leu Leu Asp Thr Val Tyr His Gly Leu Gin Gly 580 585 590 _J26: _ This paper size applies to the Chinese National Standard (CNS) A4 specification (210X297 mm) 509722 Printed by the Central Standard Flat Staff Consumer Cooperative of the Ministry of Economic Affairs A7 B7 V. Description of Invention (3ά

Phe Asp Lys Asp Lys Ala Asp Leu Leu His Val Met Ala Pro Tyr lie 595 600 605

Ala Ala Thr Leu Gin Leu Ser Ser Glu Asn Val Ala His Ser Val Leu 610 615 620

Leu Trp Ala Asp Lys Leu Gin Pro Gly Asp Gly Ala Met Thr Ala Glu 625 630 635 640

Lys Phe Trp Asp Trp Leu Asn Thr Lys Tyr Thr Pro Gly Ser Ser Glu 645 650 655

Ala Val Glu Thr Gin Glu His He Val Gin Tyr Cys Gin Ala Leu Ala 660 665 670

Gin Leu Glu Met Val Tyr His Ser Thr Gly He Asn Glu Asn Ala Phe 675 680 685

Arg Leu Phe Val Thr Lys Pro Glu Met Phe Gly Ala Ala Thr Gly Ala 690 695 700

Ala Pro Ala His Asp Ala Leu Ser Leu lie Met Leu Thr Arg Phe Ala 705 710 715 720

Asp Trp Val Asn Ala Leu Gly Glu Lys Ala Ser Ser Val Leu Ala Ala 725 730 735

Phe Glu Ala Asn Ser Leu Thr Ala Glu Gin Leu Ala Asp Ala Met Asn 740 745 750

Leu Asp Ala Asn Leu Leu Leu Gin Ala Ser lie Gin Ala Gin Asn His 755 760 765

Gin His Leu Pro Pro Val Thr Pro Glu Asn Ala Phe Ser Cys Trp Thr 770 775 780

Ser He Asn Thr He Leu Gin Trp Val Asn Val Ala Gin Gin Leu Asn 785 790 795 800

Val Ala Pro Gin Gly Val Ser Ala Leu Val Gly Leu Asp Tyr He Gin 805 810 815

Ser Met Lys Glu Thr Pro Thr Tyr Ala Gin Trp Glu Asn Ala Ala Gly _ -327-_ This paper size is applicable to the Chinese National Standard (CNS) A4 specification (210X 297 public directors --------- ^) -(Please read the notes on the back before filling this page)

1T 509722 A7 B7 V. Description of the invention (3ά 820 825 830

Val Leu Thr Ala Gly Leu Asn Ser Gin Gin Ala Asn Thr Leu His Ala 835 840 845

Phe Leu Asp Glu Ser Arg Ser Ala Ala Leu Ser Thr Tyr Tyr lie Arg 850 855 860

Gin Val Ala Lys Ala Ala Ala Ala He Lys Ser Arg Asp Asp Leu Tyr 865 870 875 880

Gin Tyr Leu Leu lie Asp Asn Gin Val Ser Ala Ala lie Lys Thr Thr 885 890 895

Arg He Ala Glu Ala lie Ala Ser He Gin Leu Tyr Val Asn Arg Ala 900 905 910

Leu Glu Asn Val Glu Glu Asn Ala Asn Ser Gly Val He Ser Arg Gin 915 920 925

Phe Phe He Asp Trp Asp Lys Tyr Asn Lys Arg Tyr Ser Thr Trp Ala 930 935 940

Gly Val Ser Gin Leu Val Tyr Tyr Pro Glu Asn Tyr lie Asp Pro Thr 945 950 955 960

Met Arg lie Gly Gin Thr Lys Met Met Asp Ala Leu Leu Gin Ser Val 965 970 975

Ser Gin Ser Gin Leu Asn Ala Asp Thr Val Glu Asp Ala Phe Met Ser 980 985 990 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling out this page)

Tyr Leu Thr Ser Phe Glu Gin Val Ala Asn Leu Lys Val He Ser Ala 995 1000 1005

Tyr His Asp Asn lie Asn Asn Asp Gin Gly Leu Thr Tyr Phe He Gly 1010 1015 1020

Leu Ser Glu Thr Asp Ala Gly Glu Tyr Tyr Trp Arg Ser Val Asp His 1025 1030 1035 1040

Ser Lys Phe Asn Asp Gly Lys Phe Ala Ala Asn Ala Trp Ser Glu Trp 1045 1050 1055 ___ -328 · _ This paper size applies Chinese National Standard (CNS) A4 specification (210X297 mm) 509722 A7 B7 V. Description of the invention (d

His Lys He Asp Cys Pro lie Asn Pro Tyr Lys Ser Thr lie Arg Pro 1060 1065 1070

Val lie Tyr Lys Ser Arg Leu Tyr Leu Leu Trp Leu Glu Gin Lys Glu 1075 1080 1085 lie Thr Lys Gin Thr Gly Asn Ser Lys Asp Gly Tyr Gin Thr Glu Thr 1090 1095 1100

Asp Tyr Arg Tyr Glu Leu Lys Leu Ala His He Arg Tyr Asp Gly Thr 1105 1110 1115 1120

Trp Asn Thr Pro He Thr Phe Asp Val Asn Lys Lys lie Ser Glu Leu 1125 1130 1135

Lys Leu Glu Lys Asn Arg Ala Pro Gly Leu Tyr Cys Ala Gly Tyr Gin 1140 1145 1150

Gly Glu Asp Thr Leu Leu Val Met Phe Tyr Asn Gin Gin Asp Thr Leu 1155 1160 1165

Asp Ser Tyr Lys Asn Ala Ser Met Gin Gly Leu Tyr He Phe Ala Asp 1170 1175 1180

Met Ala Ser Lys Asp Met Thr Pro Glu Gin Ser Asn Val Tyr Arg Asp 1185 1190 1195 1200

Asn Ser Tyr Gin Gin Phe Asp Thr Asn Asn Val Arg Arg Val Asn Asn 1205 1210 1215 Printed by the Staff Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling out this page)

Arg Tyr Ala Glu Asp Tyr Glu He Pro Ser Ser Val Ser Ser Arg Lys 1220 1225 1230

Asp Tyr Gly Trp Gly Asp Tyr Tyr Leu Ser Met Val Tyr Asn Gly Asp 1235 1240 1245

He Pro Thr He Asn Tyr Lys Ala Ala Ser Ser Asp Leu Lys lie Tyr 1250 1255 1260

He Ser Pro Lys Leu Arg He He His Asn Gly Tyr Glu Gly Gin Lys 1265 1270 1275 1280 _- ^ 29- This paper size applies to China National Standard (CNS) A4 (210X 297 mm) 509722 Employees of the Central Standards Bureau of the Ministry of Economic Affairs Printed by Consumer Cooperative A7 B7 V. Description of Invention (3 points

Arg Asn Gin Cys Asn Leu Met Asn Lys Tyr Gly Lys Leu Gly Asp Lys 1285 1290 1295

Phe He Val Tyr Thr Ser Leu Gly Val Asn Pro Asn Asn Ser Ser Asn 1300 1305 1310

Lys Leu Met Phe Tyr Pro Val Tyr Gin Tyr Ser Gly Asn Thr Ser Gly 1315 1320 1325

Leu Asn Gin Gly Arg Leu Leu Phe His Arg Asp Thr Thr Tyr Pro Ser 1330 1335 1340

Lys Val Glu Ala Trp He Pro Gly Ala Lys Arg Ser Leu Thr Asn Gin 1345 1350 1355 1360

Asn Ala Ala lie Gly Asp Asp Tyr Ala Thr Asp Ser Leu Asn Lys Pro 1365 1370 1375

Asp Asp Leu Lys Gin Tyr He Phe Met Thr Asp Ser Lys Gly Thr Ala 1380 1385 1390

Thr Asp Val Ser Gly Pro Val Glu He Asn Thr Ala He Ser Pro Ala 1395 1400 1405

Lys Val Gin lie He Val Lys Ala Gly Gly Lys Glu Gin Thr Phe Thr 1410 1415 1420

Ala Asp Lys Asp Val Ser lie Gin Pro Ser Pro Ser Phe Asp Glu Met 1425 1430 1435 1440

Asn Tyr Gin Phe Asn Ala Leu Glu He Asp Gly Ser Gly Leu Asn Phe 1445 1450 1455

He Asn Asn Ser Ala Ser He Asp Val Thr Phe Thr Ala Phe Ala Glu 1460 1465 1470

Asp Gly Arg Lys Leu Gly Tyr Glu Ser Phe Ser lie Pro Val Thr Leu 1475 1480 1485

Lys Val Ser Thr Asp Asn Ala Leu Thr Leu His His Asn Glu Asn Gly 1490 1495 1500

Ala Gin Tyr Met Gin Trp Gin Ser Tyr Arg Thr Arg Leu Asn Thr Leu __- 330-_ This paper size applies to China National Standard (CNS) A4 specification (210X 297 mm) (Please read the precautions on the back before filling in this Page) I — ^ 1— —ϋϋ —ϋ «w—ϋ- ^^ —- 1 —vuli I —ϋ I mi. Order 509722 Printed by A7 B7 of the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 5. Description of the Invention (32) 1505 1510 1515 1520

Phe Ala Arg Gin Leu Val Ala Arg Ala Thr Thr Gly lie Asp Thr lie 1525 1530 1535

Leu Ser Met Glu Thr Gin Asn lie Gin Glu Pro Gin Leu Gly Lys Gly 1540 1545 1550

Phe Tyr Ala Thr Phe Val He Pro Pro Tyr Asn Leu Ser Thr His Gly 1555 1560 1565

Asp Glu Arg Trp Phe Lys Leu Tyr lie Lys His Val Val Asp Asn Asn 1570 1575 1580

Ser His lie lie Tyr Ser Gly Gin Leu Thr Asp Thr Asn He Asn lie 1585 1590 1595 1600

Thr Leu Phe lie Pro Leu Asp Asp Val Pro Leu Asn Gin Asp Tyr His 1605 1610 1615

Ala Lys Val Tyr Met Thr Phe Lys Lys Ser Pro Ser Asp Gly Thr Trp 1620 1625 1630

Trp Gly Pro His Phe Val Arg Asp Asp Lys Gly lie Val Thr lie Asn 1635 1640 1645

Pro Lys Ser He Leu Thr His Phe Glu Ser Val Asn Val Leu Asn Asn 1650 1655 1660 lie Ser Ser Glu Pro Met Asp Phe Ser Gly Ala Asn Ser Leu Tyr Phe .1665 1670 1675 1680

Trp Glu Leu Phe Tyr Tyr Thr Pro Met Leu Val Ala Gin Arg Leu Leu 1685 1690 1695

His Glu Gin Asn Phe Asp Glu Ala Asn Arg Trp Leu Lys Tyr Val Trp 1700 1705 1710

Ser Pro Ser Gly Tyr lie Val His Gly Gin He Gin Asn Tyr Gin Trp 1715 1720 Π25

Asn Val Arg Pro Leu Leu Glu Asp Thr Ser Trp Asn Ser Asp Pro Leu 1730 1735 1740 _-331-_ This paper size applies to the Chinese National Standard (CNS) A4 size (210 X 297 mm) (Please read the note on the back first (Fill in this page again)

509722 A7 B7 V. Description of the invention (32¾

Asp Ser Val Asp Pro Asp Ala Val Ala Gin His Asp Pro Met His Tyr 1745 1750 1755 1760

Lys Val Ser Thr Phe Met Arg Thr Leu Asp Leu Leu He Ala Arg Gly 1765 1770 1775

Asp His Ala Tyr Arg Gin Leu Glu Arg Asp Thr Leu Asn Glu Ala Lys 1780 1785 1790

Met Trp Tyr Met Gin Ala Leu His Leu Leu Gly Asp Lys Pro Tyr Leu 1795 1800 1805

Pro Leu Ser Thr Thr Trp Ser Asp Pro Arg Leu Asp Arg Ala Ala Asp 1810 1815 1820

He Thr Thr Gin Asn Ala His Asp Ser Ala He Val Ala Leu Arg Gin 1825 1830 1835 1840

Asn lie Pro Thr Pro Ala Pro Leu Ser 1845 1849 (2) Information of the sequence identification number 50: (i) Sequence characteristics: (A) Length: 1740 base pairs (B) Type: Nucleic acid (C) Number of strands : Double-stranded (D) morphology .. Linear (ii) Molecular type. DNA (genome) (xi) Sequence description: Sequence identification number 50 (tcdAUi coding region): TTG CGC AGC GCT AAT ACC CTG ACT GAT CTC TTC CTG COG CM ATC MT 48 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (please read the precautions on the back before filling this page)

Leu Arg Ser Ala Asn Thr Leu Thr Asp Leu Phe Leu Pro Gin He Asn 15 10 15 GAA GTG ATG ATG AAT TAC TGG CAG ACA m GCT CAG AGA GTA TAC AAT 96

Glu Val Met Met Asn Tyr Trp Gin Thr Leu Ala Gin Arg Val Tyr Asn 20 25 30 CTG 03T CAT AAC CTC TCT ATC GAC GGC CAG CCG m TAT CTG CCA ATC 144

Leu Arg His Asn Leu Ser lie Asp Gly Gin Pro Leu Tyr Leu Pro He -332- This paper is again applicable to the Chinese National Standard (CNS) A4 size (210X 297 mm) 509722 A7 B7 V. Description of the invention (33) 35 40 45 TAT GCC ACA CCG GCC GAT COG AAA GOG ΤΓΑ CTC AGC GCC GCC GTT GCC 192

Tyr Ala Thr Pro Ala Asp Pro Lys Ala Leu Leu Ser Ala Ala Val Ala 50 55 60 ACT TCT CM GGT GGA GGC MG CTA CCG GM TCA ΉΤ ATG TCC CTG TGG 240

Thr Ser Gin Gly Gly Gly Gly Lys Leu Pro Glu Ser Phe Met Ser Leu Trp 65 70 75 80 CGT ITC COG CAC ATG CTG GM MT GCG OGC GGC ATG GTT AGC CAG CTC 288

Arg Phe Pro His Met Leu Glu Asn Ala Arg Gly Met Val Ser Gin Leu 85 90 95 AOC CAG TTC GGC TCC ACXi m CM AAT Απ ATC GAA CGT CAG GAC GOG 336

Thr Gin Phe Gly Ser Thr Leu Gin Asn lie lie Glu Arg Gin Asp Ala 100 105 110 GAA GCG CTC AAT GCG m ΊΤΑ CM AAT CAG GCC GCX: GAG CTG ATA TTG 384

Glu Ala Leu Asn Ala Leu Leu Gin Asn Gin Ala Ala Glu Leu lie Leu 115 120 125 ACT AAC CTG AGC Απ CAG GAC AAA ACC Απ GAA GAA TTG GAT GCC GAG 432

Thr Asn Leu Ser He Gin Asp Lys Thr He Glu Glu Leu Asp Ala Glu 130 135 140 AAA ACG GTG TTG GM AAA TCC AAA GCG GGA GCA CM TCG CGC ΉΤ GAT 480

Lys Thr Val Leu Glu Lys Ser Lys Ala Gly Ala Gin Ser Arg Phe Asp 145 150 155 160 AGC TAC GGC AAA CTG TAC GAT GAG AAT ATC AAC GCX: GC3T GAA AAC CM 528

Ser Tyr Gly Lys Leu Tyr Asp Glu Asn lie Asn Ala Gly Glu Asn Gin Printed by the Consumer Cooperatives of the Central Standards Bureau, Ministry of Economic Affairs (Please read the notes on the back before filling out this page) 165 170 175 GCC ATG ACG CTA CGA GOG TCC GCC GCC GGG CTT ACC ACG GCA GTT CAG 576

Ala Met Thr Leu Arg Ala Ser Ala Ala Gly Leu Thr Thr Ala Val Gin 180 185 190 GCA TCC CGT CTG GCC GGT GCG GCG GCT GAT CTG GTG CCT MC ATC TTC 624

Ala Ser Arg Leu Ala Gly Ala Ala Ala Asp Leu Val Pro Asn He Phe 195 200 205 GGC TTT GCC GGT GGC GGC AGC CGT TGG GGG GCT ATC GCT GAG GCG ACA 672 _ -333-_ This paper size applies to Chinese national standards (CNS ) A4 specification (210X297 mm) 509722 A7 _B7_ V. Description of the invention (33)

Gly Phe Ala Gly Gly Gly Ser Arg Trp Gly Ala lie Ala Glu Ala Thr 210 215 220 GGT TAT GTG ATG GAA TTC TCC GCG AAT GTT ATG AAC ACC GAA GCG GAT 720

Gly Tyr Val Met Glu Phe Ser Ala Asn Val Met Asn Thr Glu Ala Asp 225 230 235 240 AAA ATT AGC CAA TCT GAA ACC TAC CGT CGT CGC CX3T CAG GAG TGG GAG 768

Lys He Ser Gin Ser Glu Thr Tyr Arg Arg Arg Arg Gin Glu Trp Glu 245 250 255 ATC CAG CGG AAT AAT GCC GAA GCG GAA TTG AAG CAA ATC GAT GCT CAG 816

He Gin Arg Asn Asn Ala Glu Ala Glu Leu Lys Gin lie Asp Ala Gin 260 265 270 CTC AAA TCA CTC GCT GTA CGC OGC GAA GCC GCC GTA TTG CAG AM ACC 864

Leu Lys Ser Leu Ala Val Arg Arg Glu Ala Ala Val Leu Gin Lys Thr 275 280 285 AGT CTG AAA ACC CM CAA GAA CAG ACC CM TCT CM TTG GCC TTC CTG 912

Ser Leu Lys Thr Gin Gin Glu Gin Thr Gin Ser Gin Leu Ala Phe Leu 290 295 300 CM CGT MG TTC AGC MT CAG GOG m TAC AAC TGG CTG CGT GGT OGA 960

Gin Arg Lys Phe Ser Asn Gin Ala Leu Tyr Asn Trp Leu Arg Gly Arg 305 310 315 320 CTG GCG GCG ATT TAC TTC CAG TTC TAC GAT TTG GCC GTC GCG CGT TGC 1008

Leu Ala Ala lie Tyr Phe Gin Phe Tyr Asp Leu Ala Val Ala Arg Cys 325 330 335 CTG ATG GCA GAA CAA GCT TAC CGT TGG GAA CTC AAT GAT GAC TCT GCC 1056 (Notes on the back then fill out this page)

Leu Met Ala Glu Gin Ala Tyr Arg Trp Glu Leu Asn Asp Asp Ser Ala 340 345 350 CGC TTC Απ AAA COG GGC GCC TGG CAG GGA ACC TAT GCC GGT CTG CTT 1104

Arg Phe He Lys Pro Gly Ala Trp Gin Gly Thr Tyr Ala Gly Leu Leu 355 360 365 GCA GGT GAA ACC TTG ATC CTC AGT CTG GCA CAA ATG GAA GAC GCT CAT 1152

Ala Gly Glu Thr Leu Met Leu Ser Leu Ala Gin Met Glu Asp Ala His 370 375 380 _-334- This paper size applies to the Chinese National Standard (CNS) A4 specification (210X 297 mm) 509722 A7 B7 V. Description of the invention (33 ^ CTG AAA CGC GAT AAA CX3C GCA TTA GAG GTT GAA CGC ACA GTA TCXJ CTG 1200

Leu Lys Arg Asp Lys Arg Ala Leu Glu Val Glu Arg Thr Val Ser Leu 385 390 395 400 GCC GAA GIT TAT GCA GGA TTA CCA AAA GAT MC GOT (XA ΊΤΓ TCC CTG 1248

Ala Glu Val Tyr Ala Gly Leu Pro Lys Asp Asn Gly Pro Phe Ser Leu 405 410 415 GCT CAG GAA ATT GAC MG CTG GTG ACT CM GGT TCA GGC AGT GCC GGC 1296

Ala Gin Glu lie Asp Lys Leu Val Ser Gin Ser Gly Ser Ala Gly 420 425 430 ACT GGT AAT MT MT TTG GCG TTC GGC GCC GGC ACG GAC ACT AAA ACC 1344

Ser Gly Asn Asn Asn Leu Ala Phe Gly Ala Gly Thr Asp Thr Lys Thr 435 440 445 TCT TTG CAG GCA TCA GTT TCA TTC C5CT GAT TTG AAA Απ CX3T GAA GAT 1392

Ser Leu Gin Ala Ser Val Ser Phe Ala Asp Leu Lys He Arg Glu Asp 450 455 460 TAC CCG GCA TOG CTT GGC AAA Απ OGA CGT ATC AAA CAG ATC AGC GTC 1440

Tyr Pro Ala Ser Leu Gly Lys He Arg Arg lie Lys Gin lie Ser Val 465 470 475 480 ACMTG CCC GCG CTA CTG GGA CCG TAT CAG GAT GTA CAG GCA ATA TTG 1488

Thr Leu Pro Ala Leu Leu Gly Pro Tyr Gin Asp Val Gin Ala lie Leu 485 490 495 TCT TAC GGC GAT AM GCC GGA TTA GCT MC GGC TGT GAA GOG CTG GCA 1536

Ser Tyr Gly Asp Lys Ala Gly Leu Ala Asn Gly Cys Glu Ala Leu Ala 500 505 510 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (please read the notes on the back before filling this page) GTT TCT CAC GGT ATG AAT GAC AGC GGC CM TTC CAG CTC GAT TTC AAC 1584

Val Ser His Gly Met Asn Asp Ser Gly Gin Phe Gin Leu Asp Phe Asn 515 520 525 GAT GGC AAA TTC CTG CCA TTC GM GGC ATC GCC Απ GAT CM GGC AOG 1632

Asp Gly Lys Phe Leu Pro Phe Glu Gly He Ala He Asp Gin Gly Thr 530 535 540 CTG ACA CTG AGC TTC CCA AAT GCA TCT ATG CCG GAG AAA GGT AAA CM 1680

Leu Thr Leu Ser Phe Pro Asn Ala Ser Met Pro Glu Lys Gly Lys Gin 545 550 555 560 _ -335-_ This paper size applies to China National Standard (CNS) A4 specifications (210X297 mm) 509722 A7 B7 V. Description of the invention ( 33 > GCC ACT ATG ΤΓΑ AAA ACC CTG AAC GAT ATC Απ TTG CAT ATT OGC TAC 1728 Ala Thr Met Leu Lys Thr Leu Asn Asp lie lie lie lie lie lie lie lie arg Tyr 565 570 575 ACC ATT AAA TAA 1740 Thr lie Lys *** 5资料 Data of Sequence Identification Number 51: (i) Sequence characteristics: (A) Length: 579 amino acids (B) Type: amino acid (C) Number of strands: single strand (D) Form: linear (ii) ) Fractal type or: protein (xi) sequence description: sequence identification number 51 (TcdAiii):

Leu Arg Ser Ala Asn Thr Leu Thr Asp Leu Phe Leu Pro Gin He Asn 15 10 15

Glu Val Met Met Asn Tyr Trp Gin Thr Leu Ala Gin Arg Val Tyr Asn 20 25 30

Leu Arg His Asn Leu Ser He Asp Gly Gin Pro Leu Tyr Leu Pro He 35 40 45

Tyr Ala Thr Pro Ala Asp Pro Lys Ala Leu Leu Ser Ala Ala Val Ala 50 55 60

Thr Ser Gin Gly Gly Gly Gly Lys Leu Pro Glu Ser Phe Met Ser Leu Trp Printed by the Staff Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling out this page) 65 70 75 80

Arg Phe Pro His Met Leu Glu Asn Ala Arg Gly Met Val Ser Gin Leu 85 90 95

Thr Gin Phe Gly Ser Thr Leu Gin Asn lie He Glu Arg Gin Asp Ala 100 105 110

Glu Ala Leu Asn Ala Leu Leu Gin Asn Gin Ala Ala Glu Leu lie Leu 115 120 125 _ -336 · This paper size applies to Chinese National Standard (CNS) A4 specifications (210X 297 mm) 509722 A7 B7 V. Description of the invention (33 >

Thr Asn Leu Ser lie Gin Asp Lys Thr lie Glu Glu Leu Asp Ala Glu 130 135 140

Lys Thr Val Leu Glu Lys Ser Lys Ala Gly Ala Gin Ser Arg Phe Asp 145 150 155 160 (Please read the notes on the back before filling this page)

Ser Tyr Gly Lys Leu Tyr Asp Glu Asn lie Asn Ala Gly Glu Asn Gin 165 170 175

Ala Met Thr Leu Arg Ala Ser Ala Ala Gly Leu Thr Thr Ala Val Gin 180 185 190

Ala Ser Arg Leu Ala Gly Ala Ala Ala Asp Leu Val Pro Asn lie Phe 195 200 205

Gly Phe Ala Gly Gly Gly Ser Arg Trp Gly Ala He Ala Glu Ala Thr 210 215 220

Gly Tyr Val Met Glu Phe Ser Ala Asn Val Met Asn Thr Glu Ala Asp 225 230 235 240

Lys lie Ser Gin Ser Glu Thr Tyr Arg Arg Arg Arg Gin Glu Trp Glu 245 250 255

He Gin Arg Asn Asn Ala Glu Ala Glu Leu Lys Gin lie Asp Ala Gin 260 265 270

Leu Lys Ser Leu Ala Val Arg Arg Glu Ala Val Leu Gin Lys Thr 275 280 285

Ser Leu Lys Thr Gin Gin Glu Gin Thr Gin Ser Gin Leu Ala Phe Leu 290 295 300 Printed by the Consumer Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs

Gin Arg Lys Phe Ser Asn Gin Ala Leu Tyr Asn Trp Leu Arg Gly Arg 305 310 315 320

Leu Ala Ala He Tyr Phe Gin Phe Tyr Asp Leu Ala Val Ala Arg Cys 325 330 335

Leu Met Ala Glu Gin Ala Tyr Arg Trp Glu Leu Asn Asp Asp Ser Ala 340 345 350

Arg Phe He Lys Pro Gly Ala Trp Gin Gly Thr Tyr Ala Gly Leu Leu __- 337-_ This paper size applies to China National Standard (CNS) A4 specification (21〇X 297 mm) 509722 A7 B7 V. Description of invention (33 ^ 355 360 365

Ala Gly Glu Thr Leu Met Leu Ser Leu Ala Gin Met Glu Asp Ala His 370 375 380

Leu Lys Arg Asp Lys Arg Ala Leu Glu Val Glu Arg Thr Val Ser Leu 385 390 395 400

Ala Glu Val Tyr Ala Gly Leu Pro Lys Asp Asn Gly Pro Phe Ser Leu 405 410 415

Ala Gin Glu He Asp Lys Leu Val Ser Gin Gly Ser Gly Ser Ala Gly 420 425 430

Ser Gly Asn Asn Asn Leu Ala Phe Gly Ala Gly Thr Asp Thr Lys Thr 435 440 445

Ser Leu Gin Ala Ser Val Ser Phe Ala Asp Leu Lys He Arg Glu Asp 450 455 460

Tyr Pro Ala Ser Leu Gly Lys He Arg Arg He Lys Gin lie Ser Val 465 470 475 480

Thr Leu Pro Ala Leu Leu Gly Pro Tyr Gin Asp Val Gin Ala lie Leu 485 490 495

Ser Tyr Gly Asp Lys Ala Gly Leu Ala Asn Gly Cys Glu Ala Leu Ala 500 505 510

Val Ser His Gly Met Asn Asp Ser Gly Gin Phe Gin Leu Asp Phe Asn 515 520 525 Printed by the Staff Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling out this page)

Asp Gly Lys Phe Leu Pro Phe Glu Gly lie Ala lie Asp Gin Gly Thr 530 535 540

Leu Thr Leu Ser Phe Pro Asn Ala Ser Met Pro Glu Lys Gly Lys Gin 545 550 555 560

Ala Thr Met Leu Lys Thr Leu Asn Asp He He Leu His lie Arg Tyr 565 570 575

Thr lie Lys *** 579 _J38: _ This paper size applies to the Chinese National Standard (CNS) A4 specification (210X 297 mm) 509722 A7 B7 V. Description of the invention (3ά ⑵Serial Identification Number No. 52: (i) Sequence features: (A) Length: 5,532 base pairs (B) Type: Nucleic acid (C) Number of strands: Double strand (D) Morphology: Linear (ii) Molecular type: DNA (genome) (xi) Sequence identification number 52 (tcbAH coding area): ΤΤΓ ATA CM GGT TAT ACT GAT CTC m (OT MT OCT GCT GAT AAC TAT 48

Phe lie Gin Gly Tyr Ser Asp Leu Phe Gly Asn Arg Ala Asp Asn Tyr 1 5 10 15 GCC GCG CCG GGC TCG GTT GCA TCG ATG TTC TCA CCG GCG GCT TAT TTG 96

Ala Ala Pro Gly Ser Val Ala Ser Met Phe Ser Pro Ala Ala Tyr Leu 20 25 30 ACG GAA ITC TAC CCT GM GCC AM MC TTG CAT GAC AGC AGC TCA Απ 144

Thr Glu Leu Tyr Arg Glu Ala Lys Asn Leu His Asp Ser Ser Ser He 35 40 45 TAT TAC CTA GAT AM CCT CGC CCG GAT m GCA AGC m ATG CTC AGC 192

Tyr Tyr Leu Asp Lys Arg Arg Pro Asp Leu Ala Ser Leu Met Leu Ser 50 55 60 CAG AAA AAT ATG GAT GAG GM Απ TCA ACG CTC (O CTC TCT AAT GAA 240

Gin Lys Asn Met Asp Glu Glu lie Ser Thr Leu Ala Leu Ser Asn Glu 65 70 75 80 TTG TXK: CTT GCC GGG ATC GAA ACA AAA ACA GGA AAA TCA CAA GAT GAA 288 (Read the notes on the back before filling out this page)

Leu Cys Leu Ala Gly He Glu Thr Lys Thr Gly Lys Ser Gin Asp Glu 85 90 95 GTG ATG GAT ATG TTG TCA ACT TAT 0GT m AGT GGA GAG ACA CCT TAT 336

Val Met Asp Met Leu Ser Thr Tyr Arg Leu Ser Gly Glu Thr Pro Tyr 100 105 110 CAT CAC GCT TAT GAA ACT GTT CX3T GAA ATC GTT CAT GAA CGT GAT CCA 384

His His Ala Tyr Glu Thr Val Arg Glu lie Val His Glu Arg Asp Pro 115 120 125 __ · 339- This paper size applies to the Chinese National Standard (CNS) A4 specification (210X297 mm) 509722 A7 B7 Employees of the Central Standards Bureau of the Ministry of Economic Affairs Printed by the cooperative V. Description of the invention TTG GGT Απ AGC TGC CAT ATT TGG CCA GM CTG TAT 480 Pro Val Thr Leu Leu Gly He Ser Ser His He Pro Pro Glu Leu Tyr 145 150 155 160 MC TTG CTG ATT GAG GAG ATC CCG GM AM GAT GM GCC GCG CTT GAT 528 Asn Leu Leu He Glu Glu He Pro Glu Lys Asp Glu Ala Ala Leu Asp 165 170 175 ACG CTT TAT AAA ACA AAC TIT GGC GAT Απ ACT ACT GCT CAG m ATG 576 Thr Leu Tyr Lys Thr Asn Phe Gly Asp lie Thr Thr Ala Gin Leu Met 180 185 190 TCC CCA ACT TAT CTG GCC CGG TAT TAT GGC GTC TCA CCG GM GAT Απ 624 Ser Pro Ser Tyr Leu Ala Arg Tyr Tyr Gly Val Ser Pro Glu Asplie 195 200 205 GCC TAC GTG ACG ACT TCA TTA TC A CAT GTT GGA TAT AGC AGT GAT Απ 672 Ala Tyr Val Thr Thr Ser Leu Ser His Val Gly Tyr Ser Ser Asp He 210 215 220 CTG GTT ATT CCG TTG GTC GAT GGT GTG GGT AAG ATG GAA GTA GTT CGT 720 Leu Val He Pro Leu Val Asp Gly Val Gly Lys Met Glu Val Val Arg 225 230 235 240 GTT ACC CGA ACA CTA TCG GAT AAT TAT ACC AGT CAG ACG AAT TAT ATT 768 Val Thr Arg Thr Pro Ser Asp Asn Tyr Thr Ser Gin Thr Asn Tyr He 245 250 255 GAG CTG TAT CCA CAG GGT GGC GAC AAT TAT TTG ATC AAA TAC AAT CTA 816 Glu Leu Tyr Pro Gin Gly Gly Asp Asn Tyr Leu He Lys Tyr Asn Leu 260 265 270 AGC AAT AGT TTT GGT TTG GAT GAT TIT TAT CTG CM TAT AAA GAT GGT 864 Ser Asn Ser Phe Gly Leu Asp Asp Phe Tyr Leu Gin Tyr Lys Asp Gly 275 280 285 TCC GCT GAT TGG ACT GAG ATT GCC CAT AAT CCC TAT CCT GAT ATG GTC 912 Ser Ala Asp Trp Thr Glu He Ala His Asn Pro Tyr Pro Asp Met Val 290 295 300 __- 340-_ This paper size applies to China National Standard (CNS) A4 specification (210X297 mm) (Please read the note on the back J # ▼ item before filling in. Write this page)

* 1T 509722 A7 B7 V. Description of the invention (33> ΑΤΑ AAT CAA AAG TAT GAA TCA CAG GCG ACA ATC AAA CGT AC3T GAC TCT 960

He Asn Gin Lys Tyr Glu Ser Gin Ala Thr He Lys Arg Ser Asp Ser 305 310 315 320 GAC AAT ATA CTC ACT ATA GGG m CAA AGA TGG CAT AGC GGT AGT TAT 1008

Asp Asn He Leu Ser lie Gly Leu Gin Arg Trp His Ser Gly Ser Tyr 325 330 335 MT m GCC GCC GCC AAT ΉΤ AAA Απ GAC CAA TAC TCC CCG AAA GCT 1056

Asn Phe Ala Ala Ala Ala Asn Phe Lys He Asp Gin Tyr Ser Pro Lys Ala 340 345 350 TTC CTG CTT AM ATG AAT MG GCT Απ CGG TTG CTC AAA GCT ACC GGC 1104

Phe Leu Leu Lys Met Asn Lys Ala He Arg Leu Leu Lys Ala Thr Gly 355 360 365 CTC TCT m GCT ACG TTG GAG CGT ATT GIT GAT AGT GTT AAT AGC ACT 1152

Leu Ser Phe Ala Thr Leu Glu Arg He Val Asp Ser Val Asn Ser Thr 370 375 380 AAA TCC ATC ACG GTT GAG GTA m AAC AAG GTT TAT OGG GTA AAA TTC 1200

Lys Ser lie Thr Val Glu Val Leu Asn Lys Val Tyr Arg Val Lys Phe 385 390 395 400 TAT Απ GAT CGT TAT GGC ATC AGT GAA GAG ACA GCC GCT ATT TTG GCT 1248

Tyr lie Asp Arg Tyr Gly He Ser Glu Glu Thr Ala Ala lie Leu Ala 405 410 415 AAT Απ AAT ATC TCT CAG CAA GCT GTT GGC AAT CAG CTT AGC CAG ΤΓΤ 1296

Asn lie Asn He Ser Gin Gin Ala Val Gly Asn Gin Leu Ser Gin Phe 420 425 430 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling out this page) GAG CAA CTA ΤΓΤ AAT CAC (XG CCG CTC AAT GGT Απ CGC TAT GAA ATC 1344

Glu Gin Leu Phe Asn His Pro Pro Leu Asn Gly lie Arg Tyr Glu lie 435 440 445 AGT GAG GAC AAC TCC AM CAT CTT CCT AAT (XT GAT CTG AAC CTT AAA 1392

Ser Glu Asp Asn Ser Lys His Leu Pro Asn Pro Asp Leu Asn Leu Lys 450 455 460 CCA GAC AGT ACC GGT GAT GAT CM CGC MG GCG GTT m AAA CGC GCG 1440

Pro Asp Ser Thr Gly Asp Asp Gin Arg Lys Ala Val Leu Lys Arg Ala ___ J4L · _ This paper size applies to Chinese National Standard (CNS) A4 specification (21〇X: 297 mm) 509722 A7 B7 V. Description of the invention (33) 465 470 475 480 ΤΊΤ CAG GTT AAC GCC AGT GAG TTG TAT CAG ATG TTA TTG ATC ACT GAT 1488

Phe Gin Val Asn Ala Ser Glu Leu Tyr Gin Met Leu Leu lie Thr Asp 485 490 495 CGT AAA GAA GAC GGT GTT ATC AAA AAT AAC m GAG AAT ITG TCT GAT 1536

Arg Lys Glu Asp Gly Val lie Lys Asn Asn Leu Glu Asn Leu Ser Asp 500 505 510 CTG TAT TTG GTT AGT TTG CTG GCC CAG ATT CAT MC CTG ACT Απ GCT 1584

Leu Tyr Leu Val Ser Leu Leu Ala Gin lie His Asn Leu Thr lie Ala 515 520 525 GAA TTG MC ATT TTG TTG GTG ATT TGT GGC TAT GGC GAC ACC MC ATT 1632

Glu Leu Asn He Leu Leu Val He Cys Gly Tyr Gly Asp Thr Asn He 530 535 540 TAT CAG Απ ACC GAC GAT AAT ΤΓΑ GCC AAA ATA GTG GAA ACA TTG TTG 1680

Tyr Gin He Thr Asp Asp Asn Leu Ala Lys He Val Glu Thr Leu Leu 545 550 555 560 TGG ATC ACT CM TGG TTG AAG ACC CM AAA TGG ACA GTT ACC GAC CTG 1728

Trp He Thr Gin Trp Leu Lys Thr Gin Lys Trp Thr Val Thr Asp Leu 565 570 575 ΊΤΓ CTG ATG ACC ACG GCC ACT TAC AGC ACC ACT m AOG CCA GAA ATT 1776

Phe Leu Met Thr Thr Ala Thr Tyr Ser Thr Thr Leu Thr Pro Glu He 580 585 590 AGC MT CTG ACG GCT ACG TTG TCT TCA ACT TTG CAT GGC AAA GAG AGT 1824

Ser Asn Leu Thr Ala Thr Leu Ser Ser Thr Leu His Gly Lys Glu Ser Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling this page) 595 600 605 CTG ATT GGG GAA GAT CTG AAA AGA GCA ATG GCG CCT TGC TTC ACT TCG 1872

Leu He Gly Glu Asp Leu Lys Arg Ala Met Ala Pro Cys Phe Thr Ser 610 615 620 GCTITG CAT TTG ACT TCT CAA GAA GTT GCG TAT GAC 〇Ό CTG TTG TGG 1920

Ala Leu His Leu Thr Ser Gin Glu Val Ala Tyr Asp Leu Leu Leu Trp 625 630 635 640 ATA GAC CAG ATT CAA CCG GCA CAA ATA ACT GW GAT GGG TIT TGG GAA 1968 _-342- _ This paper standard applies to Chinese national standards ( CNS) A4 specification (210X297 mm) 509722 A7 B7 V. Description of the invention (34¾ lie Asp Gin lie Gin Pro Ala Gin lie Thr Val Asp Gly Phe Trp Glu 645 650 655 GAA GTG CM ACA ACA CCk kCC AGC TTG AAG GTG ATT ACC ΉΤ GCT CAG 2016

Glu Val Gin Thr Thr Pro Thr Ser Leu Lys Val He Thr Phe Ala Gin 660 665 670 GTG CTG GCA CM TTG AGC CTG ATC TAT CGT OGT Απ GGG m AGT GM 2064

Val Leu Ala Gin Leu Ser Leu He Tyr Arg Arg He Gly Leu Ser Glu 675 680 685 ACG GM CTG TCA CTG ATC GTG ACT CM TCT TCT CTG CTA GTG GCA GGC 2112

Thr Glu Leu Ser Leu He Val Thr Gin Ser Ser Leu Leu Val Ala Gly 690 695 700 AAA AGC ATA CTG GAT CAC GGT CTG m ACC CTG ATG GCC TTG GM GGT 2160

Lys Ser He Leu Asp His Gly Leu Leu Thr Leu Met Ala Leu Glu Gly 705 710 715 720 ΉΤ CAT ACC TGG GTT MT GGC KG GGG CM CAT GCC TCC TTG ATA TTG 2208

Phe His Thr Trp Val Asn Gly Leu Gly Gin His Ala Ser Leu lie Leu 725 730 735 GCG GCG TTG AAA GAC GGA GCC TTG ACA GTT ACC GAT GTA GCA CM GCT 2256

Ala Ala Leu Lys Asp Gly Ala Leu Thr Val Thr Asp Val Ala Gin Ala 740 745 750 ATG AAT AAG GAG GAA TCT CTC CTA CM ATG GCA GCT AAT CAG GTG GAG 2304

Met Asn Lys Glu Glu Ser Leu Leu Gin Met Ala Ala Asn Gin Val Glu 755 760 765 MG GAT CTA ACA AAA CTG ACC AGT TGG ACA CAG Απ GAC GCT ATT CTG 2352 (Notes on the back then fill out this page)

Lys Asp Leu Thr Lys Leu Thr Ser Trp Thr Gin lie Asp Ala lie Leu 770 775 780 CM TGG TTA CAG ATG TCT TOG GCC TTG GCG GTT TCT OCA CTG GAT CTG 2400

Gin Trp Leu Gin Met Ser Ser Ala Leu Ala Val Ser Pro Leu Asp Leu 785 790 795 800 GCA GGG ATG ATG GCC CTG AAA TAT GGG ATA GAT CAT AAC TAT GCT GCC 2448

Ala Gly Met Met Ala Leu Lys Tyr Gly lie Asp His Asn Tyr Ala Ala 805 810 815 __- 343-

This paper size applies to Chinese National Standard (CNS) A4 (2l0X 297mm T 509722 Employee Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs A7 B7 341 ----- 5. Description of the invention () TGG CM GCT GCG GCG GCT GCG CTG ATG GCT GAT CAT GCT MT CAG GCA 2496

Trp Gin Ala Ala Ala Ala Ala Leu Met Ala Asp His Ala Asn Gin Ala 820 825 830 CAG AAA AAA CTG GAT GAG ACG TTC AGT AAG GCA m TGT AAC TAT TAT 2544

Gin Lys Lys Leu Asp Glu Thr Phe Ser Lys Ala Leu Cys Asn Tyr Tyr 835 840 845 ATT AAT GCT GTT GTC GAT ACT OT (O GGA GTA OGT GAT CGT AAC GGT 2592 lie Asn Ala Val Val Asp Ser Ala Ala Gly Val Arg Asp Arg Asn Gly 850 855 860 ΤΓΑ TAT ACT TAT TTG CTG Απ GAT AAT CAG GTT TCT GOC GAT GTG ATC 2640

Leu Tyr Thr Tyr Tyr Leu Leu He Asp Asn Glii Val Ser Ala Asp Val lie 865 870 875 880 ACT TCA CGT ATT GCA GAA GCT ATC GCC GC3T ATT CAA CTG TAC GTT AAC 2688

Thr Ser Arg lie Ala Glu Ala lie Ala Gly lie Gin Leu Tyr Val Asn 885 890 895 CXjG GCTTTA AAC CGA GAT GAA GGT CAG CTT GCA TCG GAC GTT AGT ACC 2736

Arg Ala Leu Asn Arg Asp Glu Gly Gin Leu Ala Ser Asp Val Ser Thr 900 905 910 CGT CAG TTC TTC ACT GAC TGG GM CGT TAC MT AAA CGT TAC AGT ACT 2784

Arg Gin Phe Phe Thr Asp Trp Glu Arg Tyr Asn Lys Arg Tyr Ser Thr 915 920 925 TGG GCT GGT GTC TCT GM CTG GTC TAT TAT CCA GM AAC TAT GTT GAT 2832

Trp Ala Gly Val Ser Glu Leu Val Tyr Tyr Pro Glu Asn Tyr Val Asp 930 935 940 CCC ACT CAG CGC ΑΠ GGG CAA ACC AAA ATG ATG GAT GCG CTG TTG CAA 2880

Pro Thr Gin Arg lie Gly Gin Thr Lys Met Met Asp Ala Leu Leu Gin 945 950 955 960 TCC ATC AAC CAG AGC CAG CTA AAT GCG GAT ACG GTG GAA GAT GCT TTC 2928

Ser He Asn Gin Ser Gin Leu Asn Ala Asp Thr Val Glu Asp Ala Phe 965 970 975 AAA ACT TAT TTG ACC AGC TIT GAG CAG GTA GCA AAT CTG AAA GTA Απ 2976

Lys Thr Tyr Leu Thr Ser Phe Glu Gin Val Ala Asn Leu Lys Val lie 980 985 990 _ -344- This paper size applies to China National Standard (CNS) A4 specification (210X297 mm) (Please read the notes on the back before filling in (This page)

509722 A7 B7 V. Description of the invention (342) AGT GCT TAC CAC GAT AAT GTG AAT GTG GAT CAA GGA m ACT TAT TTT 3024

Ser Ala Tyr His Asp Asn Val Asn Val Asp Gin Gly Leu Thr Tyr Phe 995 1000 1005 ATC GGT ATC GAC CM GCA GCT CCG GGT ACXJ TAT TAC TGG CGT AGT GTT 3072

He Gly He Asp Gin Ala Ala Pro Gly Thr Tyr Tyr Trp Arg Ser Val 1010 1015 1020 GAT CAC AGC MA TGT GAA MT GGC MG ΉΤ GCC GCT MT GCT TGG GGT 3120

Asp His Ser Lys Cys Glu Asn Gly Lys Phe Ala Ala Asn Ala Trp Gly 1025 1030 1035 1040 GAG TGG MT AAA ATT ACC TGT GCT GTC MT OCT TGG AAA AAT ATC ATC 3168

Glu Trp Asn Lys He Thr Cys Ala Val Asn Pro Trp Lys Asn He He 1045 1050 1055 CGT CCG GTT GTT TAT ATC TCC OGC ΊΤΑ TAT CTG CTA TGG CTG GAG CAG 3216

Arg Pro Val Val Tyr Met Ser Arg Leu Tyr Leu Leu Trp Leu Glu Gin 1060 1065 1070 CAA TCA MG MA AGT GAT GAT GGT AAA ACC ACG ATT TAT CM TAT AAC 3264

Gin Ser Lys Lys Ser Asp Asp Gly Lys Thr Thr He Tyr Gin Tyr Asn 1075 1080 1085 TTA AAA CTG GCT CAT ΑΊΤ OjT TAC GAC GGT AGT TGG AAT ACA CCA TIT 3312

Leu Lys Leu Ala His He Arg Tyr Asp Gly Ser Trp Asn Thr Pro Phe 1090 1095 1100 ACT ΉΤ GAT GTG ACA GAA AAG GTA AAA AAT TAC AOG TCG AGT ACT GAT 3360

Thr Phe Asp Val Thr Glu Lys Val Lys Asn Tyr Thr Ser Ser Thr Asp 1105 1110 1115 1120 Printed by the Staff Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling out this page) GCT GCT GM TCT TTA GGG TTG TAT TGT ACT GGT TAT CAA GGG GAA GAC 3408

Ala Ala Glu Ser Leu Gly Leu Tyr Cys Thr Gly Tyr Gin Gly Glu Asp 1125 1130 1135 ACT CTA TTA GTT ATG TTC TAT TCG ATG CAG AGT AGT TAT AGC TOC TAT 3456

Thr Leu Leu Val Met Phe Tyr Ser Met Gin Ser Ser Tyr Ser Ser Tyr 1140 1145 1150 ACC GAT AAT AAT GCXJ COj GTC ACT GGG CTA TAT ATTITC GCT GAT ATG 3504

Thr Asp Asn Asn Ala Pro Val Thr Gly Leu Tyr He Phe Ala Asp Met -345- This paper size applies to the Chinese National Standard (CNS) A4 (210X297 mm) 509722 Printed by the Consumer Standards Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs A7 B7 5 Description of the invention (34) 1155 1160 1165 TCA TCA GAC AAT ATG AOG AAT GCA CAA GCA ACT AAC TAT TGG AAT AAC 3552

Ser Ser Asp Asn Met Thr Asn Ala Gin Ala Thr Asn Tyr Trp Asn Asn 1170 1175 1180 AGT TAT CCG CAA ΤΤ GAT ACT GTG ATG GCA GAT CCXj GAT AGC GAC AAT 3600

Ser Tyr Pro Gin Phe Asp Thr Val Met Ala Asp Pro Asp Ser Asp Asn 1185 1190 1195 1200 AAA AAA GTC ATA ACC AGA AGA GTT AAT AAC CGT TAT GCG GAG GAT TAT 3648

Lys Lys Val lie Thr Arg Arg Val Asn Asn Arg Tyr Ala Glu Asp Tyr 1205 1210 1215 GAA Απ CCT TCC TCT GTG ACA AGT AAC AGT AAT TAT TCT TGG GGT GAT 3696

Glu lie Pro Ser Ser Val Thr Ser Asn Ser Asn Tyr Ser Trp Gly Asp 1220 1225 1230 CAC AGT TTA ACC ATG CTT TAT GGT GGT AGT GTT CCT MT ATT ACT ΉΤ 3744

His Ser Leu Thr Met Leu Tyr Gly Gly Ser Val Pro Asnlie Thr Phe 1235 1240 1245 GAA TCG GCG GCA GAA GAT m AGG CTA TCT ACC AAT ATG GCA TTG AGT 3792

Glu Ser Ala Ala Glu Asp Leu Arg Leu Ser Thr Asn Met Ala Leu Ser 1250 1255 1260 Απ Απ CAT AAT GGA TAT GCG GGA ACC OGC OjT ATA CAA TGT AAT CTT 3840 lie lie His Asn Gly Tyr Ala Gly Thr Arg Arg Arg lie Gin Asn Leu 1265 1270 1275 1280 ATG AAA CAA TAC GCT TCA m GGT GAT AAA ΉΤ ΑΤΑ Απ TAT GAT TCA 3888

Met Lys Gin Tyr Ala Ser Leu Gly Asp Lys Phe He He Tyr Asp Ser 1285 1290 1295 TCA ΤΤΓ GAT GAT GCA AAC CGT ΉΤ MT CTG GTG CCA TTG ΉΤ AAA TTC 3936

Ser Phe Asp Asp Ala Asn Arg Phe Asn Leu Val Pro Leu Phe Lys Phe 1300 1305 1310 GGA AAA GAC GAG AAC TCA GAT GAT AGT Απ TGT ATA TAT AAT GAA AAC 3984

Gly Lys Asp Glu Asn Ser Asp Asp Ser lie Cys He Tyr Asn Glu Asn 1315 1320 1325 CCT TCC TCT GAA GAT AAG AAG TGG TAT ΤΓΤ TCT TCG AAA GAT GAC AAT 4032 __ -346-_ This paper standard applies to Chinese national standards (CNS ) A4 specification (210X 297 public broadcast 1 ~ I — ^ ------ ^ equipment ------ order ------ ^ (Please read the precautions on the back before filling this page) 509722 A7 B7 V. Description of Invention (34

Pro Ser Ser Glu Asp Lys Lys Trp Tyr Phe Ser Ser Lys Asp Asp Asn 1330 1335 1340 AAA ACA GCG GAT TAT AAT GGT GGA ACT CAA TGT ATA GAT GCT GGA ACT 4080

Lys Thr Ala Asp Tyr Asn Gly Gly Thr Gin Cys lie Asp Ala Gly Thr 1345 1350 1355 1360 AGT AAC AAA GAT TTT TAT TAT AAT CTC CAG GAG Απ GAA GTA Απ AGT 4128

Ser Asn Lys Asp Phe Tyr Tyr Asn Leu Gin Glu He Glu Val He Ser 1365 1370 1375 GTT ACT GGT GGG TAT TGG TOG AGT TAT AAA ATA TCC MC CCG Απ MT 4176

Val Thr Gly Gly Gly Tyr Trp Ser Ser Tyr Lys lie Ser Asn Pro He Asn 1380 1385 1390 ATC MT ACG GGC Απ GAT AGT GCT AAA GTA AAA GTC ACC GTA AAA GCG 4224 lie Asn Thr Gly lie Asp Ser Ala Lys Val Lys Val Thr Val Lys Ala 1395 1400 1405 GGT GGT GAC GAT CAA ATC TIT ACT GCT GAT AAT AGT ACC TAT GTT CCT 4272

Gly Gly Asp Asp Gin lie Phe Thr Ala Asp Asn Ser Thr Tyr Val Pro 1410 1415 1420 CAG CAA CCG GCA CCC AGT ΉΤ GAG GAG ATG ATT TAT CAG ITC MT MC 4320

Gin Gin Pro Ala Pro Ser Phe Glu Glu Met lie Tyr Gin Phe Asn Asn 1425 1430 1435 1440 CTG ACA ATA GAT TGT AAG AAT ΤΓΑ AAT TTC ATC GAC AAT CAG GCA CAT 4368

Leu Thr He Asp Cys Lys Asn Leu Asn Phe He Asp Asn Gin Ala His 1445 1450 1455 Απ GAG Απ GAT TTC ACC GCT A0G GCA CAA GAT GGC 0GA HC TTG GGT 4416 Note on the back, please fill out this page) lie Glu lie Asp Phe Thr Ala Thr Ala Gin Asp Gly Arg Phe Leu Gly 1460 1465 1470 GCA GM ACT TIT Απ ATC CCG GTA ACT AAA AM GTT CTC GGT ACT GAG 4464

Ala Glu Thr Phe He He Pro Val Thr Lys Lys Val Leu Gly Thr Glu 1475 1480 1485 AAC GTG ATT GCG m TAT AGC GAA AAT AAC GGT GTT CAA TAT ATG CAA 4512

Asn Val lie Ala Leu Tyr Ser Glu Asn Asn Gly Val Gin Tyr Met Gin 1490 1495 1500 ___- 347- This paper size applies to the Chinese National Standard (CNS) A4 specification (210X297 mm) 509722 A7 B7 V. Description of the invention (34) ATT GGC GCA TAT CGT ACC CGT ITG MT ACG m TTC GCT CM CAG TTG 4560 lie Gly Ala Tyr Arg Thr Arg Leu Asn Thr Leu Phe Ala Gin Gin Leu 1505 1510 1515 1520 GTT AGC CGT GCT MT CGT GGC Απ GAT GCA GTG CTC A ATG GM ACT 4608

Val Ser Arg Ala Asn Arg Gly lie Asp Ala Val Leu Ser Met Glu Thr 1525 1530 1535 CAG AAT Απ CAG GAA CCG CM m GGA GCG GGC ACA TAT GTG CAG CTT 4656

Gin Asn lie Gin Glu Pro Gin Leu Gly Ala Gly Thr Tyr Val Gin Leu 1540 1545 1550 GTG TTG GAT AAA TAT GAT GAG TCT Απ CAT GGC ACT AAT AAA AGC ΤΓΤ 4704

Val Leu Asp Lys Tyr Asp Glu Ser lie His Gly Thr Asn Lys Ser Phe 1555 1560 1565 GCT ATT GAA TAT GTT GAT ΑΤΑ ΉΤ AAA GAG AAC GAT AGTTTT GTG Απ 4752

Ala lie Glu Tyr Val Asp He Phe Lys Glu Asn Asp Ser Phe Val lie 1570 1575 1580 TAT CM GGA GAA CTT AGC GAA ACA ACT CM ACT ΰπ (ΠΌ AAA GTT TTC 4800

Tyr Gin Gly Glu Leu Ser Glu Thr Ser Gin Thr Val Val Lys Val Phe 1585 1590 1595 1600 TTA TCC TAT ΤΓΤ ATA GAG GCG ACT GGA AAT AAG AAC CAC m TGG GTA 4848

Leu Ser Tyr Phe He Glu Ala Thr Gly Asn Lys Asn His Leu Trp Val 1605 1610 1615 CXJT GCT AAA TAC CM AAG GAA AOG ACT GAT AAG ATC TTG TTC GAC ΟΤΓ 4896

Arg Ala Lys Tyr Gin Lys Glu Thr Thr Asp Lys He Leu Phe Asp Arg 1620 1625 1630 ACT GAT GAG MA GAT CCG CAC GGT TGG ΉΤ CTC AGC GAC GAT CAC MG 4944

Thr Asp Glu Lys Asp Pro His Gly Trp Phe Leu Ser Asp Asp His Lys 1635 1640 1645 A (X TTT AGT GGT CTC TCT TXT GCA CAG GCA TTA AAG AAC GAC AGT GAA 4992

Thr Phe Ser Gly Leu Ser Ser Ala Gin Ala Leu Lys Asn Asp Ser Glu 1650 1655 1660 CCG ATG GAT TTC TCT GCT GGC AAT GCT CTC TAT TTC TCG GM CTG TTC 5040

Pro Met Asp Phe Ser Gly Ala Asn Ala Leu Tyr Phe Trp Glu Leu Phe 1665 1670 1675 1680 __J48: _ This paper size applies to the Chinese National Standard (CNS) A4 specification (210X297 mm) (Please read the notes on the back first 4- Re-enter the item:: Write this page) Printed by the Consumer Cooperatives of the Central Bureau of Standards of the Ministry of Economic Affairs 509722 A7 B7 V. Description of Invention (TAT TAC ACG CCG ATG ATG ATG GCT CAT CGT TTG TTG CAG GM CAG ΜΤ 5088

Tyr Tyr Thr Pro Met Met Met Ala His Arg Leu Leu Gin Glu Gin Asn 1685 1690 1695 ΉΤ GAT GCG GCG MC CAT TGG TTC CGT TAT GTC TGG ACT CCA TCC GGT 5136

Phe Asp Ala Ala Asn His Trp Phe Arg Tyr Val Trp Ser Pro Ser Gly 1700 1705 1710 TAT ATC GTT GAT GGT AAA Απ GCT ATC TAC CAC TGG AAC GTG CXJA CXXJ 5184

Tyr He Val Asp Gly Lys lie Ala lie Tyr His Trp Asn Val Arg Pro 1715 1720 1725 CTG GAA GAA GAC ACC AGT TGG AAT GCA CAA CM CTG GAC TCC ACC GAT 5232

Leu Glu Glu Asp Thr Ser Trp Asn Ala Gin Gin Leu Asp Ser Thr Asp 1730 1735 1740 CCA GAT GCT GTA GCC CM GAT GAT CCG ATG CAC TAC MG GTG GCT ACC 5280

Pro Asp Ala Val Ala Gin Asp Asp Pro Met His Tyr Lys Val Ala Thr 1745 1750 1755 1760 ΤΊΤ ATG GCG ACG TTG GAT CTG CTA ATG GCC CGT GGT GAT GCT GCT TAC 5328

Phe Met Ala Thr Leu Asp Leu Leu Met Ala Arg Gly Asp Ala Ala Tyr 1765 1770 1775 CGC CAG TTA GAG CGT GAT ACG TTG GCT GAA GCT AAA ATC TGG TAT ACA 5376

Arg Gin Leu Glu Arg Asp Thr Leu Ala Glu Ala Lys Met Trp Tyr Thr 1780 1785 1790 CAG GCG OT MT TTG GGT GAT GAG CCA CAA GTG ATG CTG AGT ACG 5424

Gin Ala Leu Asn Leu Leu Gly Asp Glu Pro Gin Val Met Leu Ser Thr 1795 1800 1805 Printed by the Staff Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling this page) ACT TGG GCT AAT CCA ACA ΉΌ GGT MT GCT GCT TCA AAA ACC ACA CAG 5472

Thr Trp Ala Asn Pro Thr Leu Gly Asn Ala Ala Ser Lys Thr Thr Gin 1810 1815 1820 CAG OT CGT CAG CAA GTG CIT ACC CAG TTG CGT CTC AAT AGC AGG C3TA 5520

Gin Val Arg Gin Gin Val Leu Thr Gin Leu Arg Leu Asn Ser Arg Val 1825 1830 1835 1840 AAA ACT CCG TTG 5532 Lys Thr Pro Leu __ This paper size applies to China National Standard (CNS) A4 size (210X 297 mm) 509722 A7 B7 V. Description of the invention (34) 1844 (2) Information of sequence identification number 53: (i) Sequence characteristics: (A) Long skin: 1844 amino acids (B) Type: amino acid (C) strand Number: Single strand (D) Morphology: Linear (ii) Molecular type: Protein (xi) Sequence description: Sequence identification number 53 (TcbAii): Characteristic starting point and endpoint description 4 No. 1) Fragment 978 990 (Serial Identification No. 23) Fragment 1387 1401 (Serial Identification No. 22) Fragment 1484 1505 (Side ID, J Identification Editor Yu 24 Yao 3 Fragment 1527 1552 (Serial Identification Number No. The 21st)

Phe He Gin Gly Tyr Ser Asp Leu Phe Gly Asn Arg Ala Asp Asn Tyr 1 5 10 15

Ala Ala Pro Gly Ser Val Ala Ser Met Phe Ser Pro Ala Ala Tyr Leu 20 25 30

Thr Glu Leu Tyr Arg Glu Ala Lys Asn Leu His Asp Ser Ser Ser He 35 40 45

Tyr Tyr Leu Asp Lys Arg Arg Pro Asp Leu Ala Ser Leu Met Leu Ser 50 55 60

Gin Lys Asn Met Asp Glu Glu lie Ser Thr Leu Ala Leu Ser Asn Glu 65 70 75 80 Printed by the Staff Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling out this page)

Leu Cys Leu Ala Gly He Glu Thr Lys Thr Gly Lys Ser Gin Asp Glu 85 90 95

Val Met Asp Met Leu Ser Thr Tyr Arg Leu Ser Gly Glu Thr Pro Tyr 100 105 110

His His Ala Tyr Glu Thr Val Arg Glu He Val His Glu Arg Asp Pro 115 120 125

Gly Phe Arg His Leu Ser Gin Ala Pro He Val Ala Ala Lys Leu Asp _-350- This paper size applies to Chinese National Standard (CNS) A4 specification (210X 297 mm) 509722 A7 B7 V. Description of the invention (34 $ 130 135 140

Pro Val Thr Leu Leu Gly He Ser Ser His He Ser Pro Glu Leu Tyr 145 150 155 160

Asn Leu Leu He Glu Glu He Pro Glu Lys Asp Glu Ala Ala Leu Asp 165 170 175

Thr Leu Tyr Lys Thr Asn Phe Gly Asp He Thr Thr Ala Gin Leu Met 180 185 190

Ser Pro Ser Tyr Leu Ala Arg Tyr Tyr Gly Val Ser Pro Glu Asp lie 195 200 205

Ala Tyr Val Thr Thr Ser Leu Ser His Val Gly Tyr Ser Ser Asp He 210 215 220

Leu Val lie Pro Leu Val Asp Gly Val Gly Lys Met Glu Val Val Arg 225 230 235 240

Val Thr Arg Thr Pro Ser Asp Asn Tyr Thr Ser Gin Thr Asn Tyr lie 245 250 255

Glu Leu Tyr Pro Gin Gly Gly Asp Asn Tyr Leu He Lys Tyr Asn Leu 260 265 270

Ser Asn Ser Phe Gly Leu Asp Asp Phe Tyr Leu Gin Tyr Lys Asp Gly 275 280 285

Ser Ala Asp Trp Thr Glu He Ala His Asn Pro Tyr Pro Asp Met Val 290 295 300 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling out this page)

He Asn Gin Lys Tyr Glu Ser Gin Ala Thr He Lys Arg Ser Asp Ser 305 310 315 320

Asp Asn He Leu Ser He Gly Leu Gin Arg Trp His Ser Gly Ser Tyr 325 330 335

Asn Phe Ala Ala Ala Asn Phe Lys lie Asp Gin Tyr Ser Pro Lys Ala 340 345 350

Phe Leu Leu Lys Met Asn Lys Ala He Arg Leu Leu Lys Ala Thr Gly 355 360 365 __ -351- _ This paper size applies to China National Standard (CNS) A4 size (210X 297 mm) Staff Consumer Cooperatives, Central Standards Bureau, Ministry of Economic Affairs Printing 509722 A7 B7 V. Description of Invention (349)

Leu Ser Phe Ala Thr Leu Glu Arg lie Val Asp Ser Val Asn Ser Thr 370 375 380

Lys Ser lie Thr Val Glu Val Leu Asn Lys Val Tyr Arg Val Lys Phe 385 390 395 400

Tyr lie Asp Arg Tyr Gly lie Ser Glu Glu Thr Ala Ala He Leu Ala 405 410 415

Asn lie Asn He Ser Gin Gin Ala Val Gly Asn Gin Leu Ser Gin Phe 420 425 430

Glu Gin Leu Phe Asn His Pro Pro Leu Asn Gly lie Arg Tyr Glu He 435 440 445

Ser Glu Asp Asn Ser Lys His Leu Pro Asn Pro Asp Leu Asn Leu Lys 450 455 460

Pro Asp Ser Thr Gly Asp Asp Gin Arg Lys Ala Val Leu Lys Arg Ala 465 470 475 480

Phe Gin Val Asn Ala Ser Glu Leu Tyr Gin Met Leu Leu He Thr Asp 485 490 495

Arg Lys Glu Asp Gly Val He Lys Asn Asn Leu Glu Asn Leu Ser Asp 500 505 510

Leu Tyr Leu Val Ser Leu Leu Ala Gin lie His Asn Leu Thr He Ala 515 520 525

Glu Leu Asn lie Leu Leu Val He Cys Gly Tyr Gly Asp Thr Asn He 530 535 540

Tyr Gin He Thr Asp Asp Asn Leu Ala Lys He Val Glu Thr Leu Leu 545 550 555 560

Trp He Thr Gin Trp Leu Lys Thr Gin Lys Trp Thr Val Thr Asp Leu 565 570 575

Phe Leu Met Thr Thr Ala Thr Tyr Ser Thr Thr Leu Thr Pro Glu lie 580 585 590 -352- This paper size applies to China National Standard (CNS) A4 specification (210X297 mm) --------- 罾 衣-(Please read the notes on the back before filling out this page) Order 509722 A7 B7 V. Description of Invention (350)

Ser Asn Leu Thr Ala Thr Leu Ser Ser Thr Leu His Gly Lys Glu Ser 595 600 605

Leu He Gly Glu Asp Leu Lys Arg Ala Met Ala Pro Cys Phe Thr Ser 610 615 620

Ala Leu His Leu Thr Ser Gin Glu Val Ala Tyr Asp Leu Leu Leu Trp 625 630 635 640 lie Asp Gin lie Gin Pro Ala Gin He Thr Val Asp Gly Phe Trp Glu 645 650 655

Glu Val Gin Thr Thr Pro Thr Ser Leu Lys Val He Thr Phe Ala Gin 660 665 670

Val Leu Ala Gin Leu Ser Leu lie Tyr Arg Arg lie Gly Leu Ser Glu 675 680 685

Thr Glu Leu Ser Leu He Val Thr Gin Ser Ser Leu Leu Val Ala Gly 690 695 700

Lys Ser lie Leu Asp His Gly Leu Leu Thr Leu Met Ala Leu Glu Gly 705 710 715 720

Phe His Thr Trp Val Asn Gly Leu Gly Gin His Ala Ser Leu lie Leu 725 730 735

Ala Ala Leu Lys Asp Gly Ala Leu Thr Val Thr Asp Val Ala Gin Ala 740 745 750

Met Asn Lys Glu Glu Ser Leu Leu Gin Met Ala Ala Asn Gin Val Glu 755 760 765 Printed by the Staff Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling out this page)

Lys Asp Leu Thr Lys Leu Thr Ser Trp Thr Gin lie Asp Ala lie Leu 770 775 780

Gin Trp Leu Gin Met Ser Ser Ala Leu Ala Val Ser Pro Leu Asp Leu 785 790 795 800

Ala Gly Met Met Ala Leu Lys Tyr Gly He Asp His Asn Tyr Ala Ala 805 810 815

Trp Gin Ala Ala Ala Ala Ala Ala Leu Met Ala Asp His Ala Asn Gin Ala -353- This paper size applies to Chinese National Standard (CNS) A4 specification (210X297 mm) 509722 A7 __B7 _ V. Description of the invention (35l) 820 825 830

Gin Lys Lys Leu Asp Glu Thr Phe Ser Lys Ala Leu Cys Asn Tyr Tyr 835 840 845 lie Asn Ala Val Val Asp Ser Ala Ala Gly Val Arg Asp Arg Asn Gly 850 855 860

Leu Tyr Thr Tyr Leu Leu lie Asp Asn Gin Val Ser Ala Asp Val lie 865 870 875 880

Thr Ser Arg lie Ala Glu Ala lie Ala Gly lie Gin Leu Tyr Val Asn 885 890 895

Arg Ala Leu Asn Arg Asp Glu Gly Gin Leu Ala Ser Asp Val Ser Thr 900 905 910

Arg Gin Phe Phe Thr Asp Trp Glu Arg Tyr Asn Lys Arg Tyr Ser Thr 915 920 925

Trp Ala Gly Val Ser Glu Leu Val Tyr Tyr Pro Glu Asn Tyr Val Asp 930 935 940

Pro Thr Gin Arg He Gly Gin Thr Lys Met Met Asp Ala Leu Leu Gin 945 950 955 960

Ser He Asn Gin Ser Gin Leu Asn Ala Asp Thr Val Glu Asp Ala Phe 965 970 975

Lys Thr Tyr Leu Thr Ser Phe Glu Gin Val Ala Asn Leu Lys Val He 980 985 990 Printed by the Staff Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling this page)

Ser Ala Tyr His Asp Asn Val Asn Val Asp Gin Gly Leu Thr Tyr Phe 995 1000 1005

He Gly He Asp Gin Ala Ala Pro Gly Thr Tyr Tyr Trp Arg Ser Val 1010 1015 1020

Asp His Ser Lys Cys Glu Asn Gly Lys Phe Ala Ala Asn Ala Trp Gly 1025 1030 1035 1040

Glu Trp Asn Lys He Thr Cys Ala Val Asn Pro Trp Lys Asn He He 1045 1050 1055 ___J54: _ This paper size applies to China National Standard (CNS) A4 specifications (210X297 public production 1 509722 A7 B7 V. Description of the invention (35j

Arg Pro Val Val Tyr Met Ser Arg Leu Tyr Leu Leu Trp Leu Glu Gin 1060 1065 1070

Gin Ser Lys Lys Ser Asp Asp Gly Lys Thr Thr lie Tyr Gin Tyr Asn 1075 1080 1085

Leu Lys Leu Ala His lie Arg Tyr Asp Gly Ser Trp Asn Thr Pro Phe 1090 1095 1100

Thr Phe Asp Val Thr Glu Lys Val Lys Asn Tyr Thr Ser Ser Thr Asp 1105 1110 1115 1120

Ala Ala Glu Ser Leu Gly Leu Tyr Cys Thr Gly Tyr Gin Gly Glu Asp 1125 1130 1135

Thr Leu Leu Val Met Phe Tyr Ser Met Gin Ser Ser Tyr Ser Ser Tyr 1140 1145 1150

Thr Asp Asn Asn Ala Pro Val Thr Gly Leu Tyr He Phe Ala Asp Met 1155 1160 1165

Ser Ser Asp Asn Met Thr Asn Ala Gin Ala Thr Asn Tyr Trp Asn Asn 1170 1175 1180

Ser Tyr Pro Gin Phe Asp Thr Val Met Ala Asp Pro Asp Ser Asp Asn 1185 1190 1195 1200

Lys Lys Val lie Thr Arg Arg Val Asn Asn Arg Tyr Ala Glu Asp Tyr 1205 1210 1215 Printed by the Staff Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling out this page)

Glu lie Pro Ser Ser Val Thr Ser Asn Ser Asn Tyr Ser Trp Gly Asp 1220 1225 1230

His Ser Leu Thr Met Leu Tyr Gly Gly Ser Val Pro Asn He Thr Phe 1235 1240 1245

Glu Ser Ala Ala Glu Asp Leu Arg Leu Ser Thr Asn Met Ala Leu Ser 1250 1255 1260

He He His Asn Gly Tyr Ala Gly Thr Arg Arg He Gin Cys Asn Leu 1265 1270 1275 1280 This paper size applies to China National Standard (CNS) A4 (21 × 297 mm) Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs 509722 A7 B7 V. Description of the invention (35i

Met Lys Gin Tyr Ala Ser Leu Gly Asp Lys Phe He He Tyr Asp Ser 1285 1290 1295

Ser Phe Asp Asp Ala Asn Arg Phe Asn Leu Val Pro Leu Phe Lys Phe 1300 1305 1310

Gly Lys Asp Glu Asn Ser Asp Asp Ser He Cys lie Tyr Asn Glu Asn 1315 1320 1325

Pro Ser Ser Glu Asp Lys Lys Trp Tyr Phe Ser Ser Lys Asp Asp Asn 1330 1335 1340

Lys Thr Ala Asp Tyr Asn Gly Gly Thr Gin Cys lie Asp Ala Gly Thr 1345 1350 1355 1360

Ser Asn Lys Asp Phe Tyr Tyr Asn Leu Gin Glu lie Glu Val He Ser 1365 1370 1375

Val Thr Gly Gly Tyr Trp Ser Ser Tyr Lys lie Ser Asn Pro He Asn 1380 1385 1390

He Asn Thr Gly He Asp Ser Ala Lys Val Lys Val Thr Val Lys Ala 1395 1400 1405

Gly Gly Asp Asp Gin lie Phe Thr Ala Asp Asn Ser Thr Tyr Val Pro 1410 1415 1420

Gin Gin Pro Ala Pro Ser Phe Glu Glu Met He Tyr Gin Phe Asn Asn 1425 1430 1435 1440

Leu Thr He Asp Cys Lys Asn Leu Asn Phe lie Asp Asn Gin Ala His 1445 1450 1455 lie Glu lie Asp Phe Thr Ala Thr Ala Gin Asp Gly Arg Phe Leu Gly 1460 1465 1470

Ala Glu Thr Phe He lie Pro Val Thr Lys Lys Val Leu Gly Thr Glu 1475 1480 1485

Asn Val lie Ala Leu Tyr Ser Glu Asn Asn Gly Val Gin Tyr Met Gin 1490 1495 1500 lie Gly Ala Tyr Arg Thr Arg Leu Asn Thr Leu Phe Ala Gin Gin Leu ---- 356-_ This paper size applies to Chinese national standards ( CNS) A4 size (210X297mm) (Please read the precautions on the back before filling this page)

509722 Printed by the Consumer Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs A7 B7 V. Description of Invention (35) 1505 1510 1515 1520

Val Ser Arg Ala Asn Arg Gly He Asp Ala Val Leu Ser Met Glu Thr 1525 1530 1535

Gin Asn He Gin Glu Pro Gin Leu Gly Ala Gly Thr Tyr Val Gin Leu 1540 1545 1550

Val Leu Asp Lys Tyr Asp Glu Ser He His Gly Thr Asn Lys Ser Phe 1555 1560 1565

Ala He Glu Tyr Val Asp lie Phe Lys Glu Asn Asp Ser Phe Val lie 1570 1575 1580

Tyr Gin Gly Glu Leu Ser Glu Thr Ser Gin Thr Val Val Lys Val Phe 1585 1590 1595 1600

Leu Ser Tyr Phe lie Glu Ala Thr Gly Asn Lys Asn His Leu Trp Val 1605 1610 1615

Arg Ala Lys Tyr Gin Lys Glu Thr Thr Asp Lys lie Leu Phe Asp Arg 1620 1625 1630

Thr Asp Glu Lys Asp Pro His Gly Trp Phe Leu Ser Asp Asp His Lys 1635 1640 1645

Thr Phe Ser Gly Leu Ser Ser Ala Gin Ala Leu Lys Asn Asp Ser Glu 1650 1655 1660

Pro Met Asp Phe Ser Gly Ala Asn Ala Leu Tyr Phe Trp Glu Leu Phe 1665 1670 1675 1680

Tyr Tyr Thr Pro Met Met Met Ala His Arg Leu Leu Gin Glu Gin Asn 1685 1690 1695

Phe Asp Ala Ala Asn His Trp Phe Arg Tyr Val Trp Ser Pro Ser Gly 1700 1705 1710

Tyr He Val Asp Gly Lys lie Ala lie Tyr His Trp Asn Val Arg Pro 1715 1720 1725

Leu Glu Glu Asp Thr Ser Trp Asn Ala Gin Gin Leu Asp Ser Thr Asp 1730 1735 1740 ----- 357-_ This paper size applies to China National Standard (CNS) A4 specifications (210X297 mm) (please read the back first) (Notes for filling in this page)

^ T i · 509722 A7 B7 V. Description of the invention (35 :)

Pro Asp Ala Val Ala Gin Asp Asp Pro Met His Tyr Lys Val Ala Thr 1745 1750 1755 1760

Phe Met Ala Thr Leu Asp Leu Leu Met Ala Arg Gly Asp Ala Ala Tyr 1765 1770 1775

Arg Gin Leu Glu Arg Asp Thr Leu Ala Glu Ala Lys Met Trp Tyr Thr 1780 1785 1790

Gin Ala Leu Asn Leu Leu Gly Asp Glu Pro Gin Val Met Leu Ser Thr 1795 1800 1805

Thr Trp Ala Asn Pro Thr Leu Gly Asn Ala Ala Ser Lys Thr Thr Gin 1810 1815 1820

Gin Val Arg Gin Gin Val Leu Thr Gin Leu Arg Leu Asn Ser Arg Val 1825 1830 1835 1840

Lys Thr Pro Leu 1844 ⑵Data of Sequence Identification Number 54: (i) Sequence characteristics: (A) Length: 1722 base pairs (B) Type: Nucleic acid (C) Number of strands: Double strand (D) Form: Linear (ii) Molecular type: DNA (genome) (xi) Sequence description: Sequence identification number 54 (tcbAHi coding region): CTA GGA ACA GCC MT TCC CTG ACC GOT m TTC CTG CCG CAG GAA AAT 48 Central Ministry of Economic Affairs Printed by the Consumer Bureau of Standards Bureau (please read the precautions on the back before filling this page)

Leu Gly Thr Ala Asn Ser Leu Thr Ala Leu Phe Leu Pro Gin Glu Asn 15 10 15 AGC AAG CTC AAA GGC TAC TO C (Xj ACA CTG GCXj CAG CGT ATG ΉΤ AAT 96

Ser Lys Leu Lys Gly Tyr Trp Arg Thr Leu Ala Gin Arg Met Phe Asn 20 25 30 TTA CGT CAT MT CTG TCG Απ GAC GGC CAG CCG CTC TCC TTG CCG CTG 144

Leu Arg His Asn Leu Ser He Asp Gly Gin Pro Leu Ser Leu Pro Leu 35 40 45 ___- JSL · _ This paper size applies to China National Standard (CNS) A4 specification (210XM7 mm) 509722 A7 B7

V. Description of the invention (W TAT GCT AAA CCG GCT GAT CCA AAA GCT TTA CTG AGT GCG GCG GTT TCA 192

Tyr Ala Lys Pro Ala Asp Pro Lys Ala Leu Leu Ser Ala Ala Val Ser 50 55 60 GCT TCT CM GGG GGA GCC GAC TTG CCG MG GOG COG CTG ACT ATT CAC 240

Ala Ser Gin Gly Gly Ala Asp Leu Pro Lys Ala Pro Leu Thr lie His 65 70 75 80 OGC ITC CCT CM ATC CTA GM GGG GCA CGG GGC TTG GTT MC CAG CTT 288

Arg Phe Pro Gin Met Leu Glu Gly Ala Arg Gly Leu Val Asn Gin Leu 85 90 95 ATA CAG TTC GCT AGT TCA CTA ΉΌ GGG TAC AGT GAG OGT CAG GAT GCG 336 lie Gin Phe Gly Ser Ser Leu Leu Gly Tyr Ser Glu Arg Gin Asp Ala 100 105 110 GAA GCT ATG AGT CM CTA CTG CM ACC CM GCC AGC GAG m ATA CTG 384

Glu Ala Met Ser Gin Leu Leu Gin Thr Gin Ala Ser Glu Leu He Leu 115 120 125 ACC AC3T Απ CGT ATG CAG GAT AAC CM TTG GCA GAG CTG GAT TCG GAA 432

Thr Ser He Arg Met Gin Asp Asn Gin Leu Ala Glu Leu Asp Ser Glu 130 135 140 AAA ACC GCC TTG CM GTC TCT ™ GCT GGA GTG CM CM CGG ΉΤ GAC 480

Lys Thr Ala Leu Gin Val Ser Leu Ala Gly Val Gin Gin Arg Phe Asp 145 150 155 160 AGC TAT AGC CM CTG TAT GAG GAG AAC ATC AAC (3CA GGT GAG CAG OGA 528

Ser Tyr Ser Gin Leu Tyr Glu Glu Asn He Asn Ala Gly Glu Gin Arg 165 170 175 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling out this page) GCG CTO GCG TTA CGC TCA GAA TCT GCT Απ GAG TCT CAG C3GA GCG CAG 576

Ala Leu Ala Leu Arg Ser Glu Ser Ala He Glu Ser Gin Gly Ala Gin 180 185 190 ATT TCC CGT ATG GCA GGC GCG GGT GTT GAT ATG GCA CCA AAT ATC TTC 624

He Ser Arg Met Ala Gly Ala Gly Val Asp Met Ala Pro Asn He Phe 195 200 205 GGC CTG GCT GAT GGC GGC ATG CAT TAT GGT GCT Απ GCC TAT GCC ATC 672

Gly Leu Ala Asp Gly Gly Met His Tyr Gly Ala lie Ala Tyr Ala lie _-359-_ This paper size applies to Chinese National Standard (CNS) A4 specifications (210X 297 mm) 509722 A7 B7 V. Description of the invention (35) 210 215 220 GCT GAC GGT Απ GAG TTC ACT GCT TCT GCC MG ATG GTT GAT GOG GAG 720

Ala Asp Gly He Glu Leu Ser Ala Ser Ala Lys Met Val Asp Ala Glu 225 230 235 240 AAA GTT GCT CACT TCG GAA ATA TAT CGC CGT CGC CGT CM GM TGG AAA 768

Lys Val Ala Gin Ser Glu He Tyr Arg Arg Arg Arg Gin Glu Trp Lys 245 250 255 ATT CAG CGT GAC AAC GCA CM GCG GAG ATT AAC CAG m AAC GOG CM 816 lie Gin Arg Asp Asn Ala Gin Ala Glu He Asn Gin Leu Asn Ala Gin 260 265 270 CTG GM TCA CTG TCT Απ CGC CGT GM GCC GCT GM ATG CM AAA GAG 864

Leu Glu Ser Leu Ser He Arg Arg Glu Ala Ala Glu Met Gin Lys Glu 275 280 285 TAC CTG AAA ACC CAG CM GCT CAG GCG CAG GCA CAA CTT ACT TO m 912

Tyr Leu Lys Thr Gin Gin Ala Gin Ala Gin Ala Gin Leu Thr Phe Leu 290 295 300 AGA AGC MA TTC AGT AAT CM GCG m TAT AGT TGG m OGA GGG OGT 960

Arg Ser Lys Phe Ser Asn Gin Ala Leu Tyr Ser Trp Leu Arg Gly Arg 305 310 315 320 ITG TCA GGT ATT TAT TTC CAG TTC TAT GAC TTG GCC GTA TCA CGT TGC 1008

Leu Ser Gly lie Tyr Phe Gin Phe Tyr Asp Leu Ala Val Ser Arg Cys 325 330 335 CIO ATG GCA GAG CAA TOC TAT CAA TGG GAA GCT AAT GAT AAT TGC Απ 1056

Leu Met Ala Glu Gin Ser Tyr Gin Trp Glu Ala Asn Asp Asn Ser lie Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling this page) 340 345 350 AGC TIT GTC AAA CCG GGT GCA TGG CM GGA ACT TAC GCC GGC m TTG 1104

Ser Phe Val Lys Pro Gly Ala Trp Gin Gly Thr Tyr Ala Gly Leu Leu 355 360 365 TGT GGA GAA GCT TTG ATA CAA AAT CTG GCA CAA ATG GAA GAG GCA TAT 1152

Cys Gly Glu Ala Leu He Gin Asn Leu Ala Gin Met Glu Glu Ala Tyr 370 375 380 CTG AAA TGG GM TCT CGC GCT TTG GAA GTA GM CGC ACG GTT TCA HG 1200 _-360-_ This paper standard applies to Chinese national standards (CNS ) A4 specification (21 OX 297 mm) 509722 A7 B7 V. Description of invention (35)

Leu Lys Trp Glu Ser Arg Ala Leu Glu Val Glu Arg Thr Val Ser Leu 385 390 395 400 GCA GTG GTT TAT GAT TCA CTG GAA GGT AAT GAT CGT TIT AAT m GCG 1248

Ala Val Val Tyr Asp Ser Leu Glu Gly Asn Asp Arg Phe Asn Leu Ala 405 410 415 GAA CM ATA (XT GCA m TTG GAT MG GGG GAG GGA ACA GCA GGA ACT 1296

Glu Gin lie Pro Ala Leu Leu Asp Lys Gly Glu Gly Thr Ala Gly Thr 420 425 430 AAA GM AAT GGG ΊΤΑ TCA TTG GCT AAT GCT ATC CTG TCA GCT TCG GTC 1344

Lys Glu Asn Gly Leu Ser Leu Ala Asn Ala lie Leu Ser Ala Ser Val 435 440 445 AAA TTG TCC GAC TTG AAA CTG GGA ACG GAT TAT CCA GAC AGT ATC GTT 1392

Lys Leu Ser Asp Leu Lys Leu Gly Thr Asp Tyr Pro Asp Ser He Val 450 455 460 GGT AGC MC MG GTT CGT OGT Απ MG CM ATC AGT GTT TOG CTA CCT 1440

Gly Ser Asn Lys Val Arg Arg He Lys Gin He Ser Val Ser Leu Pro 465 470 475 480 GCA TTG GTT GGG CCT TAT CAG GAT GTT CAG GCT ATG CTC AGC TAT GGT 1488

Ala Leu Val Gly Pro Tyr Gin Asp Val Gin Ala Met Leu Ser Tyr Gly 485 490 495 GGC AGT ACT CM TTG GCG AAA GGT TGT TCA GCG TTG GCT GTG TCT CAT 1536

Gly Ser Thr Gin Leu Pro Lys Gly Cys Ser Ala Leu Ala Val Ser His 500 505 510 GGT ACC AAT GAT AGT GGT CAG TTC CAG TTG GAT TTC AAT GAC GGC AAA 1584 (Read the notes on the back and fill out this page)

Gly Thr Asn Asp Ser Gly Gin Phe Gin Leu Asp Phe Asn Asp Gly Lys 515 520 525 TAC CTG CCA TTT GM GGT Απ GCT CTT GAT GAT CAG GGT ACA CTG AAT 1632

Tyr Leu Pro Phe Glu Gly He Ala Leu Asp Asp Gin Gly Thr Leu Asn 530 535 540 CTT CM ΤΤΓ CCG AAT GCT ACC GAC AAG CAG AAA GCA ATA TTG CM ACT 1680

Leu Gin Phe Pro Asn Ala Thr Asp Lys Gin Lys Ala lie Leu Gin Thr 545 550 555 560 _ -361- This paper size applies to the Chinese national standard (〇 see) 8 specifications (210 father 297 mm) 509722 A7 B7 V. Description of the Invention (35) ATG AGC GAT ATT ATT TTG CAT Απ CGT TAT ACC ATC CGT TM 1722

Met Ser Asp lie lie Leu His lie Arg Tyr Thr lie Arg *** 565 570 573 资料 Sequence ID No. 55: (i) Sequence characteristics: (A) Length: 573 amino acids (B) Type: Amino acid (C) Number of strands: Single strand (D) Morphology: Linear (ii) Molecular type: Protein (xi) Sequence description i: Sequence identification number 55 (TcbAi ii):

Leu Gly Thr Ala Asn Ser Leu Thr Ala Leu Phe Leu Pro Gin Glu Asn 1 5 10 15

Ser Lys Leu Lys Gly Tyr Trp Arg Thr Leu Ala Gin Arg Met Phe Asn 20 25 30

Leu Arg His Asn Leu Ser He Asp Gly Gin Pro Leu Ser Leu Pro Leu 35 40 45

Tyr Ala Lys Pro Ala Asp Pro Lys Ala Leu Leu Ser Ala Ala Val Ser 50 55 60

Ala Ser Gin Gly Gly Ala Asp Leu Pro Lys Ala Pro Leu Thr lie His 65 70 75 80

Arg Phe Pro Gin Met Leu Glu Gly Ala Arg Gly Leu Val Asn Gin Leu The policy of employee consumer cooperatives of the Central Standards Bureau of the Ministry of Economy (Please read the precautions on the back before filling out this page) 85 90 95

He Gin Phe Gly Ser Ser Leu Leu Gly Tyr Ser Glu Arg Gin Asp Ala 100 105 110

Glu Ala Met Ser Gin Leu Leu Gin Thr Gin Ala Ser Glu Leu He Leu 115 120 125

Thr Ser He Arg Met Gin Asp Asn Gin Leu Ala Glu Leu Asp Ser Glu 130 135 140 _n This paper size applies to Chinese National Standard (CNS) A4 specification (210X297 mm) 509722 A7 ____B7_ V. Description of the invention (36 ()

Lys Thr Ala Leu Gin Val Ser Leu Ala Gly Val Gin Gin Arg Phe Asp 145 150 155 160

Ser Tyr Ser Gin Leu Tyr Glu Glu Asn He Asn Ala Gly Glu Gin Arg 165 170 175

Ala Leu Ala Leu Arg Ser Glu Ser Ala lie Glu Ser Gin Gly Ala Gin 180 185 190 lie Ser Arg Met Ala Gly Ala Gly Val Asp Met Ala Pro Asn lie Phe 195 200 205

Gly Leu Ala Asp Gly Gly Met His Tyr Gly Ala lie Ala Tyr Ala lie 210 215 220

Ala Asp Gly lie Glu Leu Ser Ala Ser Ala Lys Met Val Asp Ala Glu 225 230 235 240

Lys Val Ala Gin Ser Glu He Tyr Arg Arg Arg Arg Gin Glu Trp Lys 245 250 255 lie Gin Arg Asp Asn Ala Gin Ala Glu lie Asn Gin Leu Asn Ala Gin 260 265 270

Leu Glu Ser Leu Ser lie Arg Arg Glu Ala Ala Glu Met Gin Lys Glu 275 280 285

Tyr Leu Lys Thr Gin Gin Ala Gin Ala Gin Ala Gin Leu Thr Phe Leu 290 295 300

Arg Ser Lys Phe Ser Asn Gin Ala Leu Tyr Ser Trp Leu Arg Gly Arg 305 310 315 320 Printed by the Staff Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling out this page)

Leu Ser Gly He Tyr Phe Gin Phe Tyr Asp Leu Ala Val Ser Arg Cys 325 330 335

Leu Met Ala Glu Gin Ser Tyr Gin Trp Glu Ala Asn Asp Asn Ser He 340 345 350

Ser Phe Val Lys Pro Gly Ala Trp Gin Gly Thr Tyr Ala Gly Leu Leu 355 360 365

Cys Gly Glu Ala Leu lie Gin Asn Leu Ala Gin Met Glu Glu Ala Tyr __Jffl: _ This paper size applies to China National Standard (CNS) A4 specifications (210X 297 mm) 509722 A7 B7 V. Description of the invention (36) 370 375 380

Leu Lys Trp Glu Ser Arg Ala Leu Glu Val Glu Arg Thr Val Ser Leu 385 390 395 400

Ala Val Val Tyr Asp Ser Leu Glu Gly Asn Asp Arg Phe Asn Leu Ala 405 410 415

Glu Gin He Pro Ala Leu Leu Asp Lys Gly Glu Gly Thr Ala Gly Thr 420 425 430

Lys Glu Asn Gly Leu Ser Leu Ala Asn Ala He Leu Ser Ala Ser Val 435 440 445

Lys Leu Ser Asp Leu Lys Leu Gly Thr Asp Tyr Pro Asp Ser He Val 450 455 460

Gly Ser Asn Lys Val Arg Arg lie Lys Gin lie Ser Val Ser Leu Pro 465 470 475 480

Ala Leu Val Gly Pro Tyr Gin Asp Val Gin Ala Met Leu Ser Tyr Gly 485 490 495

Gly Ser Thr Gin Leu Pro Lys Gly Cys Ser Ala Leu Ala Val Ser His 500 505 510

Gly Thr Asn Asp Ser Gly Gin Phe Gin Leu Asp Phe Asn Asp Gly Lys 515 520 525

Tyr Leu Pro Phe Glu Gly lie Ala Leu Asp Asp Gin Gly Thr Leu Asn 530 535 540 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the precautions on the back before filling out this page)

Leu Gin Phe Pro Asn Ala Thr Asp Lys Gin Lys Ala lie Leu Gin Thr 545 550 555 560

Met Ser Asp lie He Leu His lie Arg Tyr Thr He Arg *** 565 570 573 (2) Information of the sequence identification number 56 (i) Sequence characteristics: (A) Length · 2994 base pairs (B) Type: Nucleic acid (C) Number of strands: double strand __ -364- _ This paper size applies to China National Standard (CNS) A4 specifications (210X297 mm) 509722 A7 B7 V. Description of the invention (36) (D) Form: linear (ii) Molecular type: DNA (genome) (xi) Sequence description: Sequence identification number 56 (tccA) 1 ATC MT CM CTC GCC AGT CCC CTG ATT TCC CGC ACC GM GAG ATC CAC 48 1 Met Asn Gin Leu Ala Ser Pro Leu lie Ser Arg Thr Glu Glu He His 16 49 MC m CCC GGT AAA TTG ACC GAT CTT GGT TAT ACC TCA GTG ΤΓΤ GAT 96 17 Asn Leu Pro Gly Lys Leu Thr Asp Leu Gly Tyr Thr Ser Val Phe Asp 32 97 GTG GTA CGT ATG CGG CGT GAG CGT ΉΤ Απ OGT GAG CAT GGT GCT GAT 144 33 Val Val Arg Met Pro Arg Glu Arg Phe He Arg Glu His Arg Ala Asp 48 145 CTC GGG CGC AGT GCT GM AAA ATG TAT GAC CTG GCA GTG GGC TAT GCT 192 49 Leu Gly Arg Ser Ala Glu Lys Met Tyr Asp Leu Ala Val Gly Tyr Ala 64 193 CAT CAGGTGm CA C CAT ΉΤ OGC OGT MTTCT CTT AGT GM GCT GTT 240 65 His Gin Val Leu His His Phe Arg Arg Asn Ser Leu Ser Glu Ala Val 80 241 CAG TTT GGC TTG AGA AGT COG TTC TCC GTA TCA GGC CCG GAT TAC GCC 288 81 Gin Phe Gly Leu Arg Ser Pro Phe Ser Val Ser Gly Pro Asp Tyr Ala 96 289 MTCAGTTTOTGATGCAMCACGGGTTGGAAAGATMAGCACCAAGT 336 97 Asn Gin Phe Leu Asp Ala Asn Thr Gly Trp Lys Asp Lys Ala Pro Ser 112 337 GGATCACCGGMGCCCC AGA ProgATGCGACT GPG Val Ala Tyr Leu Thr His He 128 385 TAT CAA TTG GCC CIT GAA CAG GAA AAG AAT GGC GCC ACT ACC Απ ATG 432 .129 Tyr Gin Leu Ala Leu Glu Gin Glu Lys Asn Gly Ala Thr Thr lie Met 144 Central Bureau of Standards Printed by the employee consumer cooperative (please read the notes on the back before filling this page) 433 MTACGCTGGCGGAGCGTCGCCCCGATCrGGGTGCTTrGmATTMT 480 145 Asn Thr Leu Ala Glu Arg Arg Pro Asp Leu Gly Ala Leu Leu He Asn 160 481 GAT AAA GCA ATC AAT GAG GTG ATA TTG CAG TTG GTC AAT GAA 528 161 Asp Lys Ala lie Asn Glu Val lie Pro Gin L eu Gin Leu Val Asn Glu 176 529 ATT CTG TCC AAA GCT ATT CAG MG MA CTG AGT TTG ACT GAT CTG GAA 576 177 He Leu Ser Lys Ala lie Gin Lys Lys Leu Ser Leu Thr Asp Leu Glu 192 577 GCG GTA MC GCC AGA CTT TCC ACT ACC OGT TAC CCG AAT MT CTG CCG 624 193 Ala Val Asn Ala Arg Leu Ser Thr Thr Arg Tyr Pro Asn Asn Leu Pro 208 _ > 365- This paper size applies to the Chinese National Standard (CNS) A4 specification (210 X297 mm) ) 509722 A7 B7 V. Description of the invention (36) 625 TAT CAT TAT GGT CAT CAG CAG ATT CAG ACA GCT CM TCG GTA TTG GGT 672 209 Tyr His Tyr Gly His Gin Gin lie Gin Thr Ala Gin Ser Val Leu Gly 224 673 ACT ACG TTG CM GAT ATC ACT TTG CCA CAG ACG CTG GAT CTG CCG CM 720 225 Thr Thr Leu Gin Asp He Thr Leu Pro Gin Thr Leu Asp Leu Pro Gin 240 721 AAC TTC TGG GCA ACA GCA AAA GGA AAA CTG AGC GAT ACG ACT GCC AGT 768 241 Asn Phe Trp Ala Thr Ala Lys Gly Lys Leu Ser Asp Thr Thr Ala Ser 256 769 GCTTTG ACC CGA CTG CM ATC ATG GCG AGT CAG ΠΤ TOG CCA GAG CAG 816 257 Ala Leu Thr Arg Leu Gin He Met Ala Ser Gin Phe Pro Glu Gin 272 817 CAG AAA ATC Απ ACG GAG ACT GTC GGT CAG GAT TTC TAT CAG CTT AAC 864 273 Gin Lys He lie Thr Glu Thr Val Gly Gin Asp Phe Tyr Gin Leu Asn 288 865 TAT GGT GAC AGT TO CTT ACT GTG MT AGT TTC AGC GAC ATG ACC ATA 912 289 Tyr Gly Asp Ser Ser Leu Thr Val Asn Ser Phe Ser Asp Met Thr lie 304 913 ATG ACT GAT CGA ACA AGT TTG ACT GTA CCC CAG GTA GM CTG ATG TTG 960 305 Met Thr Asp Arg Thr Ser Leu Thr Val Pro Gin Val Glu Leu Met Leu 320 961 TGTTCAACTGTCGGAGGTTCTACGGTTGTTAAGTCTGATAATGTGAGT 1008 321 Cys Ser Thr Val Gly Gly Ser Thr Val Val Lys Ser Asp Asn Val Ser 336 1009 TCTGGTGACla Pyr Thr AG Pro GGCITA CGC AGC 352 1057 CATGCCGGTMGCCGGAGGCGAITACCCrGAGTCGCAGTGGTGOGGAG 1104 353 His Ala Gly Lys Pro Glu Ala He Thr Leu Ser Arg Ser Gly Ala Glu 368 Printed by the Staff Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (please read the precautions on the back before filling this page) 1105 GCGCATTITGCTCTGACACACMTMC His Phe Ala Leu Thr Val Asn Asn Leu Thr Asp Asp Lys Leu Asp 384 1153 CGTATTAACCGCACAGTGCGCCTGCAAAAATGGCTGAATCTGCCTTAT 1200 385 Arg He Asn Arg Thr Val Arg Leu Gin Lys Trp Lep Asu Leu Pro Tyr 400 1201 GAGGATAnGACC TGACTATGATATGuAT Asu Ala Glu Thr Gly 416 1249 MT ACC GOj CTG TCG ATG AAC GAC MT ACG 〇Ό CGT ATG TO (X3A GTG 1296 417 Asn Thr Ala Leu Ser Met Asn Asp Asn Thr Leu Arg Met Leu Gly Val 432 __- 366- Applicable to this paper standard China National Standard (CNS) A4 specification (210X 297 mm) 509722 A7 B7 V. Description of the invention (36) 1297 TrCAAACATTATCAGGCGMGTATGGTGTrAGCGCTAAACMTrTGCr 1344 433 Phe Lys His Tyr Gin Ala Lys Tyr Gly Val Ser Ala Lys Gin Phe AlaCCCGACGCTGCGGCTGGCGCTGTGGC Trp Leu Arg Val Val Ala Pro Phe Ala He Thr Pro Ala Thr Pro 464 1393 TTTTTAGACCMGTGTnMCTCCGTCGGCACCTTTGATACACCGTTr 1440 465 Phe Leu Asp Gin Val Phe Asn Ser Val Gly Thr Phe Asp Thr Pro Phe 480 1441 GTGATAGATAATCAGGATTGT TTGACCACCGGGGGCGAT 1488 481 Val lie Asp Asn Gin Asp Phe Val Tyr Thr Leu Thr Thr Gly Gly Asp 496 1489 GGGGCGCGTGTTMGCATATCAGCACGGCACTGGGCCrCMTCATOGT 1536 497 GGG ALC LeC GTC LeTC GTC LeTC GTC ALC Ala Asp Asn He Ala Arg Gin Gin Gly Asn Val 528 1585 ACGCMAGCACACTCAACTGTMTCTGnTGTGGTGTCAGCnTCTAC 1632 529 Thr Gin Ser Thr Leu Asn Cys Asn Leu Phe Val Val Ser Ala Phe Tyr Lea Asr Val 544 1633 CGTCTGGCTMTTTGGGG Le Ag ArgCACATUAG Glu Ser Phe 560 1681 TGTGCCTTGGTTGATCGAmGATGCAGGTACAGGCATCGTCTGGCAG 1728 561 Cys Ala Leu Val Asp Arg Leu Asp Ala Gly Thr Gly He Val Trp Gin 576 1729 CMTTGGCAGGGAAACC Central G Proval Pros Gin Prog Proc Printed by the Bureau ’s Consumer Cooperatives (please read the notes on the back before filling this page) 1777 CTGGCGGCGGATATTCTGAGTTTGCTGC MGCGCTAAGTGCGAnGCT 1824 593 Leu Ala Ala Asp He Leu Ser Leu Leu Gin Ala Leu Ser Ala He Ala 608 1825 CMTGGCMCMCAGCACGATmGMTTTTCAGCACTGCrTTTGCrG 1872 609 Gin TrpGin Proc PGA SerGA ALC Leu ALC Leu ALC Leu He Ser Thr Ser Gin Gly Thr Asp Asp Gin Leu 640 1921 MCTTTATCCGTCMGTGTGGCAGMCCTAGGCAGTACGnTGTGGGT 1968 641 Asn Phe He Arg Gin Val Trp Gin Asn Leu Gly Ser Thr Phe Val Gly 656 1969 GCAACATTGTTGTCCCGCAGTGCCGCCGCGCGACCACC (210X297 mm) 509722 A7 B7 V. Description of the invention (36j 657 Ala Thr Leu Leu Ser Arg Ser Gly Ala Pro Leu Val Asp Thr Asn Gly 672 2017 CACGCTATTGACTGGTITGCrCTGCTCTCAGAGGGTMTAGTCCGCTT 2064 673 His Ala lie Asp Trp P Ser Ala Leu Pro Leu 688 2065 ATCGATMGGTTGGTCrGGTGACTGATGCrGGCATACMAGTGTTATA 2112 689 lie Asp Lys Val Gly Leu Val Thr Asp Ala Gly lie Gin Ser Val lie 704 2113 GCAACGGTGGTCMTACA CMAGCTTATCTGATGMGATMGAAGCTG 2160 705 Ala Thr Val Val Asn Thr Gin Ser Leu Ser Asp Glu Asp Lys Lys Leu 720 2161 GCAATCACTACTCTGACTMTACGTrGMTCAGGTACAGAAAACTCM Valt GCT AGC TGT Leu Thr Asn Thr LeinAGG GGC 2 Ser Leu Leu Ala Gin Thr Leu Asn Val Ser Gin 752 2257 TCACTGCCTGCGTTATrGTTGOGCTGGAGTGGACMACAAOCTACCAG 2304 753 Ser Leu Pro Ala Leu Leu Leu Arg Trp Ser Gly Gin Thr Thr Thr Tyr Tyr ALC Leu Leu Leu Leu Leu Arg Trp Ser Gly Gin Thr Thr Thr ATC LeC Leo Leu Leu Leu Arg 2 Thr Ala Ala 784 2353 GATATTCCCGCTGACTATCTGCGTCAAmCGTGMGTGGTAOGCCGC 2400 785 Asp He Pro Ala Asp Tyr Leu Arg Gin Leu Arg Glu Val Val Arg Arg 800 2401 TCCTTGTTGACCCMCMTTCACGCTGAGTCCTGCAATGGTGCMAOC 2448 801 Ser Leu Leu Thr Gin Gin Phe Thr Leu Ser Pro Ala Met Val Gin Thr 816 2449 TTGCTGGACTATCCAGCCTATTTTGGCGCTTCCGCAGMACAGTGACC 2496 817 Leu Leu Asp Tyr Pro Ala Tyr Phe Gly Ala Ser Ala Glu Thr Val Thr 832 Printed by the Consumer Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs (please read the precautions on the back before filling out this page) 2497 GATATCAGTTTGTGGATGCITTATACCCTGAGCTGTTATAGCGATm 2544 833 Asp He Ser Leu Trp Met Leu Tyr Thr Leu Ser Cys Tyr Ser Asp Leu 848 2545 TrGCTCAGGATCGGTACC Leu Leu Gin Met Gly Glu Ala Gly Gly Thr Glu Asp Asp Val Leu Ala 864 2593 TACmCGCACAGCTMTGCTACCACTrgGCGCATATCrGATGCT 2640 865 Tyr Leu Arg Thr Ala Asn Ala Thru ATG 880GTAGAGGCAGGCAGGCGCGCGCAC Gly Trp Glu Val Asn Glu Leu Gin 896 __- 368- This paper size applies to Chinese National Standard (CNS) A4 specifications (210X297 mm) 509722 A7 B7 V. Description of the invention (d 2689 GCCGCTTGGTCGGTATTGGGCGGGAnGCCMAACCACACCGCMCGCTG 2736 897 Ala Ala Trp Ser Val Leu Gle Gly He Ala Lys Thr Thr Pro Gin Leu 912 2737 GATGCGCTTCrGCGTTTGCAACAGGCACAGMCCAAACTGGTCTTGGC 2784 913 Asp Ala Leu Leu Arg Leu Gin Gin Ala Gin Asn Gin Thr Gly Leu Gly 928 2785 GTTACACAGCMCAGCMGGCTATCTCCTGAGTCGTGACAGTGATTAT 2832 929 Val Thr Gin Gin Gin Gin Gin Gin Gly Gyr Tyr Leu Leu Ser Arg Asp Ser Asp Tyr 944 2833 ACCCTTTGGCMAGCACCGGTCAGGCGCGCTGTGGTCTCTGGTCTATCCCAT 2880 945 Thr Gin Leu Trin G

2881 GTC AAG GGC AGT AAC TGA GCATGC5CAGA GCTCACTACC TGkGTGGm TGATIT 2934 961 Val Lys Gly Ser Asn End 965 2935 TTCOGTATGG CCTAATGAGG CTATITCTAA ACOGCCATTT AAGTAAGGCA GATAAmTG 2994 ⑵Sequence ID No. 57: Information (I) Amino acid (B) type: amino acid (C) form: linear (ii) molecular type or: protein (xi) sequence description: sequence identification number 57 (TccA peptide) characteristic beginning and end description 1 10 sequence identification No. 8 1 Met Asn Gin Leu Ala Ser Pro Leu He Ser Arg Thr Glu Glu He His 16 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (please read the precautions on the back before filling this page) 17 Asn Leu Pro Gly Lys Leu Thr Asp Leu Gly Tyr Thr Ser Val Phe Asp 32 33 Val Val Arg Met Pro Arg Glu Arg Phe lie Arg Glu His Arg Ala Asp 48 49 Leu Gly Arg Ser Ala Glu Lys Met Tyr Asp Leu Ala Val Gly Tyr Ala 64 65 His Gin Val Leu His His Phe Arg Arg Asn Ser Leu Ser Glu Ala Val 80 — ___- 369- This paper size applies to China National Standard (CNS) A4 (210X297 mm) 509722 A 7 B7 V. Description of the invention (367) 81 Gin Phe Gly Leu Arg Ser Pro Phe Ser Val Ser Gly Pro Asp Tyr Ala 96 97 Asn Gin Phe Leu Asp Ala Asn Thr Gly Trp Lys Asp Lys Ala Pro Ser 112 113 Gly Ser Pro Glu Ala Asn Asp Ala Pro Val Ala Tyr Leu Thr His lie 128 129 Tyr Gin Leu Ala Leu Glu Gin Glu Lys Asn Gly Ala Thr Thr lie Met 144 145 Asn Thr Leu Ala Glu Arg Arg Pro Asp Leu Gly Ala Leu Leu lie Asn 160 161 Asp Lys Ala lie Asn Glu Val He Pro Gin Leu Gin Leu Val Asn Glu 176 177 lie Leu Ser Lys Ala lie Gin Lys Lys Leu Ser Leu Thr Asp Leu Glu 192 193 Ala Val Asn Ala Arg Leu Ser Thr Thr Arg Tyr Pro Asn Asn Asn Asn Leu Pro 208 209 Tyr His Tyr Gly His Gin Gin He Gin Thr Ala Gin Ser Val Leu Gly 224 225 Thr Thr Leu Gin Asp lie Thr Leu Pro Gin Thr Leu Asp Leu Pro Gin 240 241 Asn Phe Trp Ala Thr Ala Lys Gly Lys Leu Ser Asp Thr Thr Ala Ser 256 257 Ala Leu Thr Arg Leu Gin He Met Ala Ser Gin Phe Ser Pro Glu Gin 272 273 Gin Lys lie lie Thr Glu Thr Val Gly Gin Asp Phe Tyr Gin Leu Asn 288 289 Tyr Gly Asp Ser Ser Leu Thr Val As n Ser Phe Ser Asp Met Thr He 304 305 Met Thr Asp Arg Thr Ser Leu Thr Val Pro Gin Val Glu Leu Met Leu 320 321 Cys Ser Thr Val Gly Gly Ser Thr Val Val Lys Ser Asp Asn Val Ser 336 337 Ser Gly Asp Thr Thr Ala Thr Pro Phe Ala Tyr Gly Ala Arg Phe He 352 353 His Ala Gly Lys Pro Glu Ala lie Thr Leu Ser Arg Ser Gly Ala Glu 368 369 Ala His Phe Ala Leu Thr Val Asn Asn Leu Thr Asp Asp Lys Leu Asp 384 385 Arg He Asn Arg Thr Val Arg Leu Gin Lys Trp Leu Asn Leu Pro Tyr 400 401 Glu Asp He Asp Leu Leu Val Thr Ser Ala Met Asp Ala Glu Thr Gly 416 -370- This paper size applies to China National Standard (CNS) A4 specifications (21 OX2W mm) I-I — (Please read the notes on the back before filling this page)

1T printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs 509722 printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs A7 B7 V. Invention Description (368) 417 Asn Thr Ala Leu Ser Met Asn Asp Asn Thr Leu Arg Met Leu Gly Val 432 433 Phe Lys His Tyr Gin Ala Lys Tyr Gly Val Ser Ala Lys Gin Phe Ala 448 449 Gly Trp Leu Arg Val Val Ala Pro Phe Ala lie Thr Pro Ala Thr Pro 464 465 Phe Leu Asp Gin Val Phe Asn Ser Val Gly Thr Phe Asp Thr Pro Phe 480 481 Val lie Asp Asn Gin Asp Phe Val Tyr Thr Leu Thr Thr Gly Gly Asp 496 497 Gly Ala Arg Val Lys His He Ser Thr Ala Leu Gly Leu Asn His Arg 512 513 Gin Phe Leu Leu Leu Ala Asp Asn He Ala Arg Gin Gin Gly Asn Val 528 529 Thr Gin Ser Thr Leu Asn Cys Asn Leu Phe Val Val Ser Ala Phe Tyr 544 545 Arg Leu Ala Asn Leu Ala Arg Thr Leu Gly lie Asn Pro Glu Ser Phe 560 561 Cys Ala Leu Val Asp Arg Leu Asp Ala Gly Thr Gly lie Val Trp Gin 576 577 Gin Leu Ala Gly Lys Pro Thr He Thr Val Pro Gin Lys Asp Ser Pro 592 593 Leu Ala Ala Asp He Leu Ser Leu Leu Gin Ala Leu Ser Ala lie Ala 608 609 Gin Trp Gin Gin Gin His Asp Leu Glu Phe Ser Ala Leu Leu Leu Leu 624 625 Leu Ser Asp Asn Pro He Ser Thr Ser Gin Gly Thr Asp Asp Gin Leu 640 641 Asn Phe He Arg Gin Val Trp Gin Asn Leu Gly Ser Thr Phe Val Gly 656 657 Ala Thr Leu Leu Ser Arg Ser Gly Ala Pro Leu Val Asp Thr Asn Gly 672 673 His Ala lie Asp Trp Phe Ala Leu Leu Ser Ala Gly Asn Ser Pro Leu 688 689 lie Asp Lys Val Gly Leu Val Thr Asp Ala Gly He Gin Ser Val lie 704 705 Ala Thr Val Val Asn Thr Gin Ser Leu Ser Asp Glu Asp Lys Lys Leu 720 721 Ala lie Thr Thr Leu Thr Asn Thr Leu Asn Gin Val Gin Lys Thr Gin 736 737 Gin Gly Val Ala Val Ser Leu Leu Ala Gin Thr Leu Asn Val Ser Gin 752 753 Ser Leu Pro Ala Leu Leu Leu Arg Trp Ser Gly Gin Thr Thr Tyr Gin 768 -371- This paper size applies to the Chinese National Standard (CNS) A4 specification (210X297) Li) (Please read the notes on the back before filling this page)

Printed by 509722 A7 B7 Five 'Invention Note (369) " 769 Trp Leu Ser Ala Thr Trp Ala Leu Lys Asp Ala Val Lys Thr Ala Ala 784 785 Asp lie Pro Ala Asp Tyr Leu Arg Gin Leu Arg Glu Val Val Arg Arg 800 801 Ser Leu Leu Thr Gin Gin Phe Thr Leu Ser Pro Ala Met Val Gin Thr 816 817 Leu Leu Asp Tyr Pro Ala Tyr Phe Gly Ala Ser Ala Glu Thr Val Thr 832 833 Asp He Ser Leu Trp Met Leu Tyr Thr Leu Ser Cys Tyr Ser Asp Leu 848 849 Leu Leu Gin Met Gly Glu Ala Gly Gly Thr Glu Asp Asp Val Leu Ala 864 865 Tyr Leu Arg Thr Ala Asn Ala Thr Thr Pro Leu Ser Gin Ser Asp Ala 880 881 Ala Gin Thr Leu Ala Thr Leu Leu Gly Trp Glu Val Asn Glu Leu Gin 896 897 Ala Ala Trp Ser Val Leu Gly Gly lie Ala Lys Thr Thr Pro Gin Leu 912 913 Asp Ala Leu Leu Arg Leu Gin Gin Ala Gin Asn Gin Thr Gly Leu Gly 928 929 Val Thr Gin Gin Gin Gin Gly Tyr Leu Leu Ser Arg Asp Ser Asp Tyr 944 945 Thr Leu Trp Gin Ser Thr Gly Gin Ala Leu Val Ala Gly Val Ser His 960 961 Val Lys Gly Ser Asn 965 Information of identification number 58: (i) Sequence characteristics: (A) Length: 4932 base pairs (B) Type: Nucleic acid (C) Number of strands: Double strand (D) Form: Linear (ii) Molecular type: DNA (genome) (xi) Sequence description: Sequence identification number 58 (tccB) 1 ATXmATCGACAATGGMAAACAACTGMTGMTCCCAGCGTGATGCG 48 1 Met Leu Ser Thr Met Glu Lys Gin Leu Asn Glu Ser Gin Arg Asp Ala 16 49 TO (ΠΌ ACTGGCTATATGAGA HTGTGGCGCG 96 17 Leu Val Thr Gly Tyr Met Asn Phe Val Ala Pro Thr Leu Lys Gly Val 32 -372- This paper size applies to China National Standard (CNS) A4 (210X297 mm) (Please read the precautions on the back before filling in this page)

509722 A7 ____ B7 V. Description of the Invention (370) 97 AGTGGTCAGCCGGTGACGGTGGMGATTTATACGMTATTTGCTGATr 144 33 Ser Gly Gin Pro Val Thr Val Glu Asp Leu Tyr Glu Tyr Leu Leu lie 48 145 GACCCGGMGT Val Val AGlu AsGlu GPGAGGluGluProg Gin Ala 64 (Please read the notes on the back before filling this page) 193 ATT GCC AGC ATA CAG CAA TAT ΑΊΌ ACT CGT CTO (Π € MC GGC TCT GAA 240 65 lie Ala Ser lie Gin Gin Tyr Met Thr Arg Leu Val Asn Gly Ser Glu 80 241 CCGGGGCGTCAGGOGATGGAGCCTTCTACAGCTMCGMTGGCGTGAT 288 81 Pro Gly Arg Gin Ala Met Glu Pro Ser Thr Ala Asn Glu Trp Arg Asp 96 289 AATGATMCCMTATGCTATCTGGGCrGCGGGGGCrGAGGTrOGAAAT 336 97 Asn Asp Asn Gin Tyr Ala lie Trp Ala Ala Gly Ala Glu Val Arg Asn 112 337 TACGCTGMMCTATATTTCACCCATCACCOGGCAGGAAAAAAGCCAT 384 113 Tyr Ala Glu Asn Tyr lie Ser Pro lie Thr Arg Gin Glu Lys Ser His 128 385 TAT TTC TOG GAG CTG GAG AGG ACT m AAT CAG AAT CGA CTC GAT CCG 432 129 Tyr Phe Ser Glu Leu Glu Thr Thr Leu Asn Gin Asn Arg Leu A sp Pro 144 433 GATCGTGTGCAGGATGCTGTnTGGCGTATCTCAATGAGTTTGAGGCA 480 145 Asp Arg Val Gin Asp Ala Val Leu Ala Tyr Leu Asn Glu Phe Glu Ala 160 481 GTG AGT AAT CTA TAT GTG CTC AGT GGT TAT ATT AAT ValT Tyr AU Tyr AU TGA TAU Ser Gly Tyr He Asn Gin Asp Lys Phe 176 Printed by the Employees' Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs 529 GACCMGCT ATCTACTACmAnGGTOGC ACTA (XACTAAACCGTAT 576 177 Asp Gin Ala He Tyr Tyr Phe He Gly Arg Thr Thr Thr Thr Lys Pro Tyr 192 577 OGCTACTGGGGAGAGAGAGAGAGAGAG Tyr Tyr Trp Arg Gin Met Asp Leu Ser Lys Asn Arg Gin Asp Pro 208 625 GCA GGG AAT CCG GTG ACG CCA AAT TGC TGG AAT GAT TGG CAG GM ATC 672 209 Ala Gly Asn Pro Val Thr Pro Asn Cys Trp Asn Asp Trp Gin Glu He 224 673 ACT TTG CCG CTG TCT GGT GAT ACG GTG CTG GAG CAT ACA GTT CGC CCG 720 225 Thr Leu Pro Leu Ser Gly Asp Thr Val Leu Glu His Thr Val Arg Pro 240 ____J73; ____ 'Paper dimensions are applicable to Chinese national standards (CNS ) A4 specification (210X297 mm) A '509722 A7 B7 V. Invention (37) 721 GTATTTTATMTGATCGACTATATGTGGCTTGGGTTGAGCGTGACCOG 768 241 Val Phe Tyr Asn Asp Arg Leu Tyr Val Ala Trp Val Glu Arg Asp Pro 256 769 GCA GTA CAG MG GAT GCTGACGGT AAA AACATCGGT AAA ACC CAT Gla Asp Asp 257 Asp Gly Lys Thr His Ala 272 817 TACAACATAMGTTTGGTTATAAACGTTATGATGATACTTGGACAGCG 864 273 Tyr Asn lie Lys Phe Gly Tyr Lys Arg Tyr Asp Asp Thr Trp Thr Ala 288 865 OOGMTACGACCACGmATGACACMCAAGCAGGGGAAAGTTCAGAA 912 289 Pro Asn Thr Thr Thr Leu Met Thr Gin Gin Ala Gly Glu Ser Ser Glu 304 913 ACACAGCGATCCAGCCTGCTGAnGATGAATCTAGCACCACATrGCGC 960 305 Thr Gin Arg Ser Ser Leu Leu He Asp Glu Ser Ser Thr Thr Leu Arg 320 961 CMOTMTCTGTTCGCTACCACCGATmACTATCGATCCXjACGGAG 1008 321 Gin Val Asn Leu Leu Ala Thr Thr Thr Asp Phe Ser He Asp Pro Thr Glu 336 100CC TGAGTGGGAGAC Tyr Gly Arg Leu Met Leu Gly Val Phe Val 352 1057 CGTCMTrTGAAGGTGATGGGGCCAATAGAMAMTAAACCCGTTGTr 1104 353 Arg Gin Phe Glu Gly Asp Gly Al a Asn Arg Lys Asn Lys Pro Val Val 368 1105 TATGGTTATCTCTATTGTGACTCAGCTTTCMTCGTCATGTTCTCAGG 1152 369 Tyr Gly Tyr Leu Tyr Cys Asp Ser Ala Phe Asn Arg His Val Leu Arg 384 1153 COGmAGTAAGMCimTGTTCAGTACT TGGuTuGuTuGuTuGuTuGuTuGu Asp 400 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (please read the precautions on the back before filling out this page) 1201 GGTCMMCAGCTTGCMTnGCGGTATACGATAAAMGTATGTAAH 1248 401 Gly Gin Asn Ser Leu Gin Phe Ala Val Tyr Asp Lys Lys Tyr Val He 416 1249 ACTMGGTTGTTGAGACCGCGTGCAGGCAA Thr Lys Val Val Thr Gly Ala Thr Glu Asp Pro Glu Asn Thr Gly Trp 432 1297 GTAAGTAAAGTTGATGACTTGAAACAAGGCACTACTGGGGCCTATGTG 1344 433 Val Ser Lys Val Asp Asp Leu Lys Gin Gly Thrp TGITCACATCACACAAC Leu His He Gin Thr Thr Thr Asn 464 -m— This paper size applies to China National Standard (CNS) A4 (210X 297 mm) 509722 A7 ____B7 V. Description of the Invention (372) 1393 GGGGATTnTAnAACCGTCATACGTITGGATATMCGATCTTGTATAT 1440 465 Gly Asp Phe He Asn Arg His Thr Phe Gly Tyr Asn Asp Leu Val Tyr 480 1441 GATTCTMGTCTGGTTnGGnTCAOGTGG TTGGGTGTGGGTGTGGTGTGGTGTGGTGTGGTGTGGTGTGGTGTGGTG 496 1489 TATCTOGATTACCATGATGGAAATTATTACACCTITCATAATGCAATA 1536 497 Tyr Leu Asp Tyr His Asp Gly Asn Tyr Tyr Thr Phe His Asn Ala lie 512 1537 ATCMCTACTATCOGTCTGGATATGGTGGTGGATCTGTTCCTMTGGA 1584 513 lie Asn Tyr Tyr Pro Ser Gly Tyr Gly Gly Gly Ser Val Pro Asn Gly 528 1585 ACGTGGGCGmGAGCMAGGAnMTGAGGGATGGGCTAnGCTCOC 1632 529 Thr Trp Ala Leu Glu Gin Arg lie Asn Glu Gly Trp Ala lie Ala Pro 544 1633 CrGCTTGATACTCrCCATACTGTTACTGTGMGGGCAGTTATATCGCT 1680 545 Leu Leu Asp Thr Leu His Thr Val Thr Val Lys Gly Ser Trp He Ala 560 1681 TGGGGGGGGAGMTCTACCTACC Tyr He Pro Asp Gly 576 1729 ACCGTGITGCTAGATTGGTTTGATAAAATAMTTrTGCrATrGGTCTT 1776 577 Thr Va l Leu Leu Asp Trp Phe Asp Lys He Asn Phe Ala lie Gly Leu 592 1777 MTAAGaTGAGTCTGTATITACGTCGCCAGATTGGCCAACACTAACC 1824 593 Asn Lys Leu Glu Ser Val Phe Thr Ser Pro Asp Trp Pro Thr Leu Thr 608 1825 ACTAT CAMTACTATCATCACCATCACCA Asp Asn Arg Lys Phe Tyr Gin 624 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (please read the notes on the back before filling out this page) 1873 GAAATCAATGCTGAGAOGGCGGATGGAOGCAACCrGTITAAACGTTAC 1920 625 Glu He Asn Ala Glu Thr Ala Asp Gly Arg Asn Leu Phe Lys Tyr 640 1921 AGTACTCMACTTTCGGACTTACCAGCGGTGCGACTTATTCTACAACT 1968 641 Ser Thr Gin Thr Phe Gly Leu Thr Ser Gly Ala Thr Tyr Ser Thr Thr 656 1969 TAT ACnTGTCT GAG GCG GAT TTCTCC ACT GAT CCGGAC AAA Tyrp Asp Aspira Asp AC Aspp Lys Asn Tyr 672 2017 CTACAGGTTTGTTTGAATGTCGTGTGGGATCATTATGACOGCCCGTCA 2064 673 Leu Gin Val Cys Leu Asn Val Val Trp Asp His Tyr Asp Arg Pro Ser 688 > 375- This paper size applies to Chinese national standards (CNS) A4 specifications (210X297 mm) 509722 A7 B7 V. Description of the invention (373) 2065 GGGAMAAAGGGGCrTATTCTTGGGTCAGTMGTGGTITAACGTCTAT 2112 689 Gly Lys Lys Gly Ala Tyr Ser Trp Val Ser Lys Trp Phe Asn Val Tyr 704 2113 GTTGCGCTGCGCGCMGGCGCGCMGMG Ser Lys Ala Pro Asp Ala lie Pro Arg Leu Val 720 2161 TCCOGTTACGATAGTAAACGTGGTCTGGTGCMTATCTGGACTrCTGG 2208 721 Ser Arg Tyr Asp Ser Lys Arg Gly Leu Val Gin Tyr Leu Asp Phe Trp 736 2209 ACCTCATCAmCCCGCGMAhr TCRu TCGMCTCTTCMCTCTTCMCT Phe Val Arg 752 2257 ACTTTGAHGAGMGGCTMTCTGGGGCTGGATAGTTTGCrGGATTAC 2304 753 Thr Leu He Glu Lys Ala Asn Leu Gly Leu Asp Ser Leu Leu Asp Tyr 768 2305 ACCTTGCAGGCAGATCCTTCTCTGGMGCAGATmGTGACTGACGGC 2352 769 Thr Leu Gin Ala Asp Pro Ser Leu Glu Ala Asp Leu Val Thr Asp Gly 784 2353 AAAAGCGMCCAATCGACTTTMTGGTTCAMCGGTCTCTATTTCTGG 2400 785 Lys Ser Glu Pro Met Asp Phe Asn Gly Ser Asn Gly Leu Tyr Phe Trp 800 2401 GAATTGTTCTITCACCTGCCGTTn TGGTrGCTACACGCTTrGCCAAC 2448 801 Glu Leu Phe Phe His Leu Pro Phe Leu Val Ala Thr Arg Phe Ala Asn 816 2449 GMCAGCMTTTTCGCCGGCACMMGAGTTTGCATTACATCTTrGAC 2496 817 Glu GCC GCG PGI Ser Pro Ala Gin Lys Ser PMTGAC HeGC TCG Lys Pro His Asn Ala Pro Ala Tyr Trp Asn Val 848 2545 CGTCCGTrGGTrGMGGAMCAGCGATTTGTCACGTCATTTGGACGAT 2592 849 Arg Pro Leu Val Glu Gly Asn Ser Asp Leu Ser Arg His Leu Asp Asp 864 (Please read the note on the back first) Please fill in this page for more information) 2593 TCTATAGACCCAGATACTCMOTTATGCrCATCCGGTGATATACCAG 2640 865 Ser lie Asp Pro Asp Thr Gin Ala Tyr Ala His Pro Val He Tyr Gin 880 2641 AAAGOGGTGTTTATrGCCTATTGTCCATCATC ACTC ALC GLA Ala Vala Ala Vala 2736 897 Met Trp Tyr Arg Gin Leu Thr Arg Asp Gly Leu Thr Gin Ala Arg Val 912 2737 TATTACMTCTGGCCGCTGAATr GCTAGGGCCTCGTCCGGATGTATOG 2784 _____- 376- This paper size applies to Chinese National Standard (CNS) A4 specification (210X297 mm) 509722 A7 B7 V. Description of invention (374) 913 Tyr Tyr Asn Leu Ala Ala Glu Leu Leu Gly Pro Arg Pro Asp Val Ser 928 2785 CTGAGTAGCATTTGGACGCCGCAAACCCTGGATACCnAGCAGCCGGG 2832 929 Leu Ser Ser He Trp Thr Pro Gin Thr Leu Asp Thr Leu Ala Ala Gly 944 2833 CMAMGCGOTmCGTGATmGAGCACCAGTTGGCTAATAGTGAT 2880 945 Gin Lys Ala Val Leu Arg Asp Phe Glu His Gin Leu Ala Asn Ser Asp 960 2881 ACCGCTTTACCCGCATTGCOGGGCOGCMTGTCAGCTACTTGAAACTG 2928 961 Thr Ala Leu Pro Ala Leu Pro Gly Arg Asn Val Ser Tyr Leu Lys Leu 976 2929 GCAGATAATGGCTACnTMTGMCCGCTCMTGTTCTGATGTTGTCT 2976 977 Ala Asp Asn Gly Tyr Phe Asn Glu Pro Leu Asn Val Leu Met Leu Ser 992 2977 CACTGGGATACGTTTG TGCTCGCGCGCACAC Leu Arg His Asn Leu 1008 3025 ACCGTrGATGGCMGCCGCTTTOGCTGCOGCTGTATGCTGCGCCrGTT 3072 1009 Thr Val Asp Gly Lys Pro Leu Ser Leu Pro Leu Tyr Ala Ala Pro Val 1024 3 073 GATCCGGTAGCGTTGTTGGCTCAGCGTGCTCAGTCCGGCAOGTrGACG 3120 1025 Asp Pro Val Ala Leu Leu Ala Gin Arg Ala Gin Ser Gly Thr Leu Thr 1040 3121 MTGGCGTCAGTGGCGCCATGTTGAOGGTGCCGCCATACCGTTTCAGC 3168 1041 Asn Gly Val Ser Gly Ala Met Leu Thr Val Pro Pro Tyr Arg Phe Ser 1056 3169 GCTATGTrGCCGCGAGCTTACAGCGCCGTGGGTACGTTGACCAGTTTT 3216 1057 Ala Met Leu Pro Arg Ala Tyr Ser Ala Val Gly Thr Leu Thr Ser Phe 1072 3217 GGTCAGAACCTGCITAGTTTGTTGGAACGTAGCGMCGAGCCTGTCM 3264 1073 Gly Gin Asn Leu Leu Ser Leu Leu Leu Glu Arg Ser Glu Arg Ala Cys Gin 1088 Note: Please fill in this page again.) 3265 GAA GAG TTG GO; CM CAG CAA CTG TTG GAT ATG TCX: AGC TAT GCC ATC 3312 1089 Glu Glu Leu Ala Gin Gin Gin Leu Leu Asp Met Ser Ser Tyr Ala lie 1104 3313 ACGTTGCMCMCAGGCGCTGGATGGATC 1TG33CTC Leu Gin Gin Gin Ala Leu Asp Gly Leu Ala Asp Arg Leu Ala 1120 3361 CTGCTAGCTAGTCAGGCTACGGCACACCACAGTGTCATGACCATTATTAC 3408 1121 Leu Leu Ala Ser Gin Al a Thr Ala Gin Gin Arg His Asp His Tyr Tyr 1136 3409 ACT CTG TAT CAG AACAAC ATC TCCAGTGCG GAA CAA CTG GTG ATG GAC 3456 1137 Thr Leu Tyr Gin Asn Asn He Ser Ser Ala Glu Gin Leu Val Met Asp 1152 ____- 377 · Paper size applies to Chinese National Standard (CNS) A4 specification (210X 297 mm) 509722 A7 B7 V. Invention Description (375) 3457 ACCCMACGTCAGCACAATCCCTGATTTCTTCTTCCACTGGTGTACAA 3504 1153 Thr Gin Thr Ser Ala Gin Ser Leu He Ser Ser Thr Gly Val Gin 1GTGATCGTCAC 3552 1169 Thr Ala Ser Gly Ala Leu Lys Val lie Pro Asn He Phe Gly Leu Ala 1184 3553 GATGGCGGCTGGOGCTATGMGGAGTAAOGGMGOGAWGCCATCGGG 3600 1185 Asp Gly Gly Serla Arg Tyr Glu GCC AMGCCCC1GGGGCCCC1MGGCC Ser Val Val Ala Glu Arg Leu Ala 1216 3649 ACCACGGAGMTTACOGCOGCOGCOGTGAAGAGTGGCMATCCMTAC 3696 1217 Thr Thr Glu Asn Tyr Arg Arg Arg Arg Glu Glu Trp Gin He Gin Tyr 1232 3697 CAGCAGGCACAGTCTGAGGTCGACGCA mCAGAAACAGTTGGATGCG 3744 1233 Gin Gin Ala Gin Ser Glu Val Asp Ala Leu Gin Lys Gin Leu Asp Ala 1248 3745 CTGGCAGTGCGCGAGMAGMAGGCGCMACTTCCCTGCGCMCAGGOGMG 3792 1249 Leu Ala CA GUC Lyc GUC Lyc AUC GUC Lyc GUC (^ ACT TACm ACT ACT OjT 3840 1265 Ala Gin Gin Val Gin He Arg Thr Met Leu Thr Tyr Leu Thr Thr Arg 1280 3841 TTCACCCAGGCGACTCTGTACCAGTGGCTGAGTGGTCMTTATCCCCGTC3TCCTC3GT128C PTC The Gin Ala Thr Leu Tyr Gyr Tyr Gyr Tin 1297 Leu Tyr Tyr Gin Ala Tyr Asp Ala Val Val Ala Leu Cys Leu Ser Ala 1312 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (please read the notes on the back before filling out this page) 3937 CAAGCTTGCTGGCAGTATGAATTGGGTGATTACGCTACCACTTITATC 3984 1313 Gin Ala Cys Trp Gin Tyr Glu Leu Gly Asp Tyr Ala Thr Thr Phe lie 1328 3985 CAGACCGGTACCTGGMCGACCATTACCGTGGTTTGCAAGTGGGGGAG 4032 1329 Gin Thr Gly Thr Trp Asn Asp Hi s Tyr Arg Gly Leu Gin Val Gly Glu 1344 4033 ACACTGCMCrCMTTTGCATCAGATGGMGOGGCCTATmGTTCGT 4080 1345 Thr Leu Gin Leu Asn Leu His Gin Met Glu Ala Ala Tyr Leu Val Arg 1360 4081 CACGMCGCCGTCTTMTGTGATCCGTACTGermany Glu Seru 378 Glu Arg Glu Arg National Standard (CNS) A4 specification (210X297 mm) 509722 A7 B7 V. Description of the invention (376) 4129 TTGGGTGATGATGGTnTGGTAAGmAAAACCGMGGCAAAGTCGAC 4176 1377 Leu Gly Asp Asp Gly Phe Gly Lys Leu Lys Thr Glu Gly Lys Val Asp 1392AGACA 4CMTCAC Ser Glu Lys Leu Phe Asp Asn Asp Tyr Pro Gly His Tyr 1408 4225 TTGCGCCAGAnAAAACTGTGTCAGTGAOGTTGCOGACGmGTCGGG 4272 1409 Leu Arg Gin lie Lys Thr Val Ser Val Thr Leu Pro Thr Val Tly Leu Val Gly 1424 4273 COGTATCMMCGTCMGCACACAAGOCAGCACrOCAOCACr Thr Ser Ser Ser lie 1440 4321 TTGTTAGCAGCAGATATCMTGGTGTTAAACGTCTCMTGATCCGACA 4368 1441 Leu Leu Ala Ala Asp He Asn Gly Val L ys Arg Leu Asn Asp Pro Thr 1456 4369 GGTAAAGAGGGTGATGCGACGCATATTGTCACCMTCTGCGTGCCAGC 4416 1457 Gly Lys Glu Gly Asp Ala Thr His He Val Thr Asn Leu Arg Ala Ser 1472 4417 CAGCAGGTGGCGCTCTCTTCTGGCATTMTGATG GinGu GinPin 1 4465 TTGCGTTTGGMGATGAGCGCTATCTATCATTTGAGGGGACTGGAGCT 4512 1489 Leu Arg Leu Glu Asp Glu Arg Tyr Leu Ser Phe Glu Gly Thr Gly Ala 1504 4513 GTTTCCAAATGGACTCITMCTrCCOGCGTTCTGTGGATGAGCATAn 4560 1505 Val Ser Lys Trp Thr Leu Asn Phe Pro Arg Ser Val Asp Glu His lie 1520 4561 GACGATMGACATTGAAAGCGGATGAGATCCAGGCCGCACTGTTGGCG 4608 1521 Asp Asp Lys Thr Leu Lys Ala Asp Glu Met Gin Ala Ala Leu Leu Ala 1536 4609 AATATGGATGATGTGCTGGTGCAGGTGCATTATACCGCCTGCGACGGC 4656 1537 Asn Met Asp Asp Val Leu Val Gin Val His Tyr Thr Ala Cys Asp Gly 1552 (Please fill in this page again)

4657 GGC GCC ACT TTC GCA AAC CAG GTC MG AAA ACA CTC TCT TM CATTMCHT 4708 1553 Gly Ala Ser Phe Ala Asn Gin Val Lys Lys Thr Leu Ser End 1565 4709 TAACTMTCCCTOGCACTCrGTrGGGATCAG-Catalog 4768TGn CGATGAGAGGTGTGTCATCATATCrAAAATCA 4CGTGNACG Applicable to China National Standard (CNS) A4 specification (210X 297 mm) 509722 A7 B7 V. Description of invention (37)

4829 TATG 4932 (2) Serial identification number 59 (please read the precautions on the back before filling this page) (i) Sequence characteristics: (A) Length: 1565 amino acids Acid (C) Form: Linear (ii) Molecular type: Protein (xi) Sequence description: Sequence identification number 59 (TccB peptide) Characteristic origin and endpoint description 1 Met Leu Ser Thr Met Glu Lys Gin Leu Asn Glu Ser Gin Arg Asp Ala 16 17 Leu Val Thr Gly Tyr Met Asn Phe Val Ala Pro Thr Leu Lys Gly Val 32 33 Ser Gly Gin Pro Val Thr Val Glu Asp Leu Tyr Glu Tyr Leu Leu lie 48 49 Asp Pro Glu Val Ala Asp Glu Val Glu Thr Ser Arg Val Ala Gin Ala 64 65 He Ala Ser He Gin Gin Tyr Met Thr Arg Leu Val Asn Gly Ser Glu 80 81 Pro Gly Arg Gin Ala Met Glu Pro Ser Thr Ala Asn Glu Trp Arg Asp 96 97 Asn Asp Asn Gin Tyr Ala He Trp Ala Ala Gly Ala Glu Val Arg Asn 112 113 Tyr Ala Glu Asn Tyr He Ser Pro lie Thr Arg Gin Glu Lys Ser His 128 129 Tyr Phe Ser Glu Leu Glu Thr Thr Leu Asn Gin Asn Arg Leu Asp Pro 144 Central Ministry of Economy 145 Asp Arg printed by Standards Bureau's Consumer Cooperative Val Gin Asp Ala Val Leu Ala Tyr Leu Asn Glu Phe Glu Ala 160 161 Val Ser Asn Leu Tyr Val Leu Ser Gly Tyr He Asn Gin Asp Lys Phe 176 177 Asp Gin Ala lie Tyr Tyr Phe He Gly Arg Thr Thr Thr Lys Pro Tyr 192 193 Arg Tyr Tyr Trp Arg Gin Met Asp Leu Ser Lys Asn Arg Gin Asp Pro 208 209 Ala Gly Asn Pro Val Thr Pro AsnCys Trp Asn Asp Trp Gin Glu He 224 225 Thr Leu Pro Leu Ser Gly Asp Thr Val Leu Glu Hi s Thr Val Arg Pro 240 This paper size applies to Chinese National Standard (CNS) A4 (210X297 mm) 509722 A7 B7 V. Description of the invention (378y Printed on the staff consumer cooperative of the Central Standards Bureau of the Ministry of Economic Affairs 241 Val Phe Tyr Asn Asp Arg Leu Tyr Val Ala Trp Val Glu Arg Asp Pro 256 257 Ala Val Gin Lys Asp Ala AspGly Lys Asn lie Gly Lys Thr His Ala 272 273 Tyr Asn He Lys PheGly Tyr Lys Arg Tyr Asp AspThr Trp Thr Leu Gin Gin Ala Gly Glu Ser Ser Glu 304 305 Thr Gin Arg Ser Ser Leu Leu He Asp Glu Ser Ser Thr Thr Leu Arg 320 321 Gin Val Asn Leu Leu Ala Thr Thr Asp Phe Ser lie Asp Pro Thr Glu 336 337 Glu Thr Asp Ser Asn Pro Tyr Gly Arg Leu Met Leu Gly Val Phe Val 352 353 Arg Gin Phe Glu Gly Asp Gly Ala Asn Arg Lys Asn Lys Pro Val Val 368 369 Tyr Gly Tyr Leu Tyr Cys Asp Ser Ala Phe Asn Arg His Val Leu Arg 384 385 Pro Leu Ser Lys Asn Phe Leu Phe Ser Thr Tyr Arg Asp Glu Thr Asp 400 401 Gly Gin Asn Ser Leu Gin Phe Ala Val Tyr Asp Lys Lys Tyr Val lie 416 417 Thr Lys Val Val Thr Gly Ala Thr Glu Asp Pro Glu Asn Thr Gly Trp 432 433 Val Ser Lys Val Asp Asp Leu Lys Gin Gly Thr Thr Gly Ala Tyr Val 448 449 Tyr He Asp Gin Asp Gly Leu Thr Leu His lie Gin Thr Thr Thr Asn 464 465 Gly Asp Phe He Asn Arg His Thr Phe Gly Tyr Asn Asp Leu Val Tyr 480 481 Asp Ser Lys Ser Gly Tyr Gly Phe Thr Trp Ser Gly Asn Glu Gly Phe 496 497 Tyr Leu Asp Tyr His Asp Gly Asn Tyr Tyr Thr Phe His Asn Ala He 512 513 lie Asn Tyr Tyr Pro Ser Gly Tyr Gly Gly Gly Ser Val Pro Asn Gly 528 529 Thr Trp Ala Leu Glu Gin Arg He Asn Glu Gly Trp Ala He Ala Pro 544 545 Leu Leu Asp Thr Leu His Thr Val Thr Val Lys Gly Ser T yr He Ala 560 561 Trp Glu Gly Glu Thr Pro Thr Gly Tyr Asn Leu Tyr He Pro Asp Gly 576 m HI · 11 LI K-(Please read the precautions on the back before filling this page)

、 1T wood paper size applies Chinese National Standard (CNS) A4 specification (210X 297 mm) 509722 A7 _____B7 _ V. Description of invention (379) 577 Thr Val LeuLeu AspTrpPhe AspLys lie AsnPhe Ala IleGlyLeu 592 593 Asn Lys Leu Glu Ser Val Phe Thr Ser Pro Asp Trp Pro Thr Leu Thr 608 609 Thr He Lys Asn Phe Ser Lys He Ala Asp Asn Arg Lys Phe Tyr Gin 624 625 Glu lie Asn Ala Glu Thr Ala Asp Gly Arg Asn Leu Phe Lys Arg Tyr 640 641 Ser Thr Gin Thr Phe Gly Leu Thr Ser Gly Ala Thr Tyr Ser Thr Thr 656 657 Tyr Thr Leu Ser Glu Ala Asp Phe Ser Thr Asp Pro Asp Lys Asn Tyr 672 673 Leu Gin Val Cys Leu Asn Val Val Trp Asp His Tyr Asp Arg Pro Ser 688 689 Gly Lys Lys Gly Ala Tyr Ser Trp Val Ser Lys Trp Phe Asn Val Tyr 704 705 Val Ala Leu Gin Asp Ser Lys Ala Pro Asp Ala He Pro Arg Leu Val 720 721 Ser Arg Tyr Asp Ser Lys Arg Gly Leu Val Gin Tyr Leu Asp Phe Trp 736 737 Thr Ser Ser Leu Pro Ala Lys Thr Arg Leu Asn Thr Thr Phe Val Arg 752 753 Thr Leu lie Glu Lys Ala Asn Leu Gly Leu Asp Ser Leu Leu Asp Tyr 768 769 Thr Leu Gin A la Asp Pro Ser Leu Glu Ala Asp Leu Val Thr Asp Gly 784 785 Lys Ser Glu Pro Met Asp Phe Asn Gly Ser Asn Gly Leu Tyr Phe Trp 800 801 Glu Leu Phe Phe His Leu Pro Phe Leu Val Ala Thr Arg Phe Ala Asn 816 .817 Glu Gin Gin Phe Ser Pro Ala Gin Lys Ser Leu His Tyr He Phe Asp 832 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (please read the precautions on the back before filling this page) 833 Pro Ala Met Lys Asn Lys Pro His Asn Ala Pro Ala Tyr Trp Asn Val 848 849 Arg Pro Leu Val Glu Gly Asn Ser Asp Leu Ser Arg His Leu Asp Asp 864 865 Ser He Asp Pro Asp Thr Gin Ala Tyr Ala His Pro Val He Tyr Gin 880 881 Lys Ala Val Phe He Ala Tyr Val Ser Asn Leu He Ala Gin Gly Asp 896 897 Met Trp Tyr Arg Gin Leu Thr Arg Asp Gly Leu Thr Gin Ala Arg Val 912 913 Tyr Tyr Asn Leu Ala Ala Glu Leu Leu Gly Pro Arg Pro Asp Val Ser 928 ___ -382- The paper size of the table applies the Chinese National Standard (CNS) A4 specification (210 X297 mm) 509722 A7 __B7_ V. Description of the invention (380) 929 Leu Ser Ser He Trp Thr Pro Gin Thr Leu Asp Thr Leu Ala Ala Gly 94 4 945 GlnLys Ala Val Leu Arg AspPheGluHis Gin Leu Ala Asn Ser Asp 960 Printed by the Consumers' Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (please read the notes on the back before filling this page) 961 Thr Ala Leu Pro Ala Leu Pro Gly Arg Asn Val Ser Tyr Leu Lys Leu 976 977 Ala Asp Asn Gly Tyr Phe Asn Glu Pro Leu Asn Val Leu Met Leu Ser 992 993 Hi s Trp Asp Thr Leu Asp A1 a Arg Leu Tyr Asn Leu Arg Hi s Asn Leu 1008 1009 Thr Val Asp Gly Lys Pro Leu Ser Leu Pro Leu Tyr Ala Ala Pro Val 1024 1025 Asp Pro Val Ala Leu Leu Ala Gin Arg Ala Gin Ser Gly Thr Leu Thr 1040 1041 Asn Gly Val Ser Gly Ala Met Leu Thr Val Pro Pro Tyr Arg Phe Ser 1056 1057 Ala Met Leu Pro Arg Ala Tyr Ser Ala Val Gly Thr Leu Thr Ser Phe 1072 1073 Gly Gin Asn Leu Leu Ser Leu Leu Glu Arg Ser Glu Arg Ala Cys Gin 1088 1089 Glu Glu Leu Ala Gin Gin Gin Leu Leu Asp Met Ser Ser Tyr Ala lie 1104 1105 Thr Leu Gin Gin Gin Ala Leu Asp Gly Leu Ala Ala Asp Arg Leu Ala 1120 1121 Leu Leu Ala Ser Gin Ala Thr Ala Gin Gin Arg His Asp His Tyr Tyr 1136 1137 Thr Leu Ty r Gin Asn Asn lie Ser Ser Ala Glu Gin Leu Val Met Asp 1152 1153 Thr Gin Thr Ser Ala Gin Ser Leu He Ser Ser Ser Thr Gly Val Gin 1168 1169 Thr Ala Ser Gly Ala Leu Lys Val He Pro Asn lie Phe Gly Leu Ala 1184 1185 Asp Gly Gly Ser Arg Tyr Glu Gly Val Thr Glu Ala lie Ala lie Gly 1200 1201 Leu Met Ala Ala Gly Gin Ala Thr Ser Val Val Ala Glu Arg Leu Ala 1216 1217 Thr Thr Glu Asn Tyr Arg Arg Arg Arg Glu Glu Trp Gin lie Gin Tyr 1232 1233 Gin Gin Ala Gin Ser Glu Val Asp Ala Leu Gin Lys Gin Leu Asp Ala 1248 1249 Leu Ala Val Arg Glu Lys Ala Ala Gin Thr Ser Leu Gin Gin Ala Lys 1264 ___- 383- This paper is for China National Standard (CNS) A4 Specification (210X 297 mm) 509722 A7 __B7 V. Invention Description (38)) 1265 Ala Gin Gin Val Gin He Arg Thr Met Leu Thr Tyr Leu Thr Thr Arg 1280 1281 Phe Thr Gin Ala Thr Leu Tyr Gin Trp Leu Ser Gly Gin Leu Ser Ala 1296 1297 Leu Tyr Tyr Gin Ala Tyr Asp Ala Val Val Ala Leu Cys Leu Ser Ala 1312 1313 Gin Ala Cys Trp Gin Tyr Glu Leu Gly Asp Tyr Ala Thr Thr Phe lie 1328 132 9 Gin Thr Gly Thr Trp Asn Asp Hi s Tyr Arg Gly Leu Gin Val Gly Glu 1344 1345 Thr Leu Gin Leu Asn Leu His Gin Met Glu Ala Ala Tyr Leu Val Arg 1360 1361 His Glu Arg Arg Leu Asn Val He Arg Thr Val Ser LeuLys Ser Leu 1376 1377 Leu Gly Asp Asp Gly Phe Gly Lys Leu Lys Thr Glu Gly Lys Val Asp 1392 1393 Phe Pro Leu Ser Glu Lys Leu Phe Asp Asn Asp Tyr Pro Gly His Tyr 1408 1409 Leu Arg Gin He Lys Thr Val Ser Val Thr Leu Pro Thr Leu Val Gly 1424 1425 Pro Tyr Gin Asn Val Lys Ala Thr Leu Thr Gin Thr Ser Ser Ser lie 1440 1441 Leu Leu Ala Ala Asp He Asn Gly Val Lys Arg Leu Asn Asp Pro Thr 1456 1457 Gly Lys Glu Gly Asp Ala Thr His lie Val Thr Asn Leu Arg Ala Ser 1472 1473 Gin Gin Val Ala Leu Ser Ser Gly He Asn Asp Ala Gly Ser Phe Glu 1488 1489 Leu Arg Leu Glu Asp Glu Arg Tyr Leu Ser Phe Glu Gly Thr Gly Ala 1504 1505 Val Ser Lys Trp Thr Leu Asn Phe Pro Arg Ser Val Asp Glu His lie 1520 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (please read the precautions on the back before filling this page) 1521 Asp Asp Lys Thr Leu Lys Ala Asp Glu Met Gin Ala Ala Leu Leu Ala 1536 1537 Asn Met Asp Asp Val Leu Val Gin Val His Tyr Thr Ala Cys Asp Gly 1552 1553 Gly Ala Ser Phe Ala Asn Gin Val Lys Lys Thr Leu Ser 1565 辨识 Sequence recognition Information of No. 60: (i) Sequence characteristics: (A) Length: 3132 base pairs (B) Type: Nucleic acid (C) Number of strands ·· Double strands ___- 384 -__ This paper size applies to China Standard (CNS) A4 specification (210X 297 mm) 509722 A7 B7 V. Description of the invention (38) (D) Form: linear (ii) Molecular type: DNA (genome) (xi) Sequence description: Sequence identification number 60 (TccC) 1 ATGAGTCCGTCTGAGACTACTCTTTATACTCMACCCCAACAGTCAGC 48 1 Met Ser Pro Ser Glu Thr Thr Leu Tyr Thr Gin Thr Pro Thr Val Ser 16 49 GTG TTA GAT MT CGC GGT CTG TCC ATT CGT GAT Απ GGT ΤΓΤ CAC OGT As 17 Val Leu Leu Ser lie Arg Asp lie Gly Phe His Arg 32 97 ATTGTAATCGGGGGGGATACTGACACCOGCGTCAOCOGTCACCAGTAT 144 33 lie Val lie Gly Gly Asp Thr Asp Thr Arg Val Thr Arg His Gin Tyr 48 145 GATGCCCGTGGACACCTGAACTACAGTAGTAGTA 192 49 Asp Ala Arg Gly His Leu Asn Tyr Ser lie Asp Pro Arg Leu Tyr Asp 64 193 GCAMGCAGGCTGATMCTCACjTAMGiXTMTTrTGTCTGGCAGCAT 240 65 Ala Lys Gin Ala Asp Asn Ser Val Lys Pro Asn Phe Val Trp Gin His 80 241 GATCAGGCGCGCGCGCCAT Leu Arg Thr Glu Ser Val Asp Ala Gly Arg 96 289 ACTGTrGCATrGMTGATAWGAAGGTCGTTOGGTAATGACAATGMT 336 97 Thr Val Ala Leu Asn Asp He Glu Gly Arg Ser Val Met Thr Met Asn 112 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (please read the notes on the back first) then fill the page) 337 GCGACCGGTGTTCGTCAGACCCGTCGCTATGAAGGCMCACCTTGCCC 384 113 Ala Thr Gly Val Arg Gin Thr Arg Arg Tyr Glu Gly Asn Thr Leu Pro 128 385 GGTCGCTrGmTCTGTGAGCGAGCMGmTCMCCMGAGAGTGCT 432 129 Gly Arg Leu Leu Ser Val Ser Glu Gin Val Phe Asn Gin Glu Ser Ala 144 433 AMGTOACAGAGCGCTITATCTGGGCTGGGAATACAACCTCXjGAGAAA 480 145 Lys Val Thr Glu Arg Phe lie Trp Ala Gly Asn Thr Thr Ser Glu Lys 160 __- 385- This paper size applies to Chinese National Standard (CNS) A4 specifications (21 0X 297 mm) 509722 A7 B7 V. Description of the Invention (383) 481 GAGTATMCCTCTCCGGTCTGTGTATACGCCACTACGACACAGCGCGAGA 528 161 Glu Tyr Asn Leu Ser Gly Leu Cys lie Arg His Tyr Asp Thr Ala Gly 176 GGC ACTGGGTGGACTCTCGCTCTCAC Gly Ala Met Leu Ser Gin 192 577 TCTCACCMTTGCTGGCGGMGGGCAGGAGGCTMCTGGAGCGGTGAC 624 193 Ser His Gin Leu Leu Ala Glu Gly Gin Glu Ala Asn Trp Ser Gly Asp 208 625 GACGMACTGly Trp Vala Thr Glu CGCT TGAGA TGCATAG Please read the notes on the back _ — 0 ▼ Please fill in this page and write this page) 673 CMAGTACCACTMTGCCATCGGGGCnTACTGACCCMACCGATGCG 720 225 Gin Ser Thr Thr Asn Ala He Gly Ala Leu Leu Thr Gin Thr Asp Ala 240 Order 721 AMGGCMTATTCAGCGTCTGGCTTA TGACGGAnGCCInGCC Arg Leu Ala Tyr Asp lie Ala Gly Gin Leu Lys 256 769 GGGAGTTGGTTGACGGTGAAAGGCCAGAGAACAGGTGAnGTTAAG 816 257 Gly Ser Trp Leu Thr Val Lys Gly Gin Ser Glu Gin Val lie Val Lys 272 817 TCCCrGAGCTGGTCAGCCGCAGGTCATMATTGCGTGMGAGCACGGT 864 273 Ser Leu Ser Trp Ser Ala Ala Gly His Lys Leu Arg Glu Glu His Gly 288 865 MCGGCGTGGTTACGGAGTACAGTTATGAGCCGGMACTCMCGTCTG 912 289 Asn Gly Val Val Thr Glu Tyr Ser Tyr Glu Pro Glu Thr Gin Arg Leu 304 913 ATAGGTATCACCACCCGGCGTGCCGAAGGGAGTCAATCAGGAGCCAGA 960 305 lie Gly He Thr Thr Arg Arg Ala Glu Gly Ser Gin Ser Gly Ala Arg 320 961 GTATTGCAGGATCTACGCTATMGTATGATCCGGTGGGGMTGTTATC 1008 ___- 386 · This paper size applies to China National Standard (CNS) A4 size (210X: 297 mm) I # Central Standards Bureau employee consumption Printed by the cooperative 509722 A7 B7 5. Description of the invention (38φ) 321 Val Leu Gin Asp Leu Arg Tyr Lys Tyr Asp Pro Val Gly Asn Val He 336 1009 AGTATCCATMTGATGCCGMGCTACCCGCTITTGGCGTMTCAGAAA 1056 337 Ser He His Asn Asp Ala Glu Ala Thr Arg Pnin Lys 352 1057 GTGGAGCOGGAGAATCGCTATGTTTATGATTCTCrGTATCAGCTTATG 1104 353 Val Glu Pro Glu Asn Arg Tyr Val Tyr Asp Ser Leu Tyr Gin Leu Met 368 1105 ACTGCGACAGGGCGTGMATGGCTAATATCGGTCAGC MAGCAACCM 1152 369 Ser Ala Thr Gly Arg Glu Met Ala Asn lie Gly Gin Gin Ser Asn Gin 384 1153 OTCCCTCACCCGTTATACCTGTTCCTACTGACGACAGCACTTATACC 385 Leu Pro Ser Pro Val lie TGT TGT TGT TGT TMT TGT TMTTTGTACC 1GTACC Tyr Thr Tyr Asp Arg Gly Gly Asn Leu Val Gin 416 1249 ATCCGACACAGTTCACCCGCGACTCAAAATAGTTACACCACAGATAIX: 1296 417 He Arg His Ser Ser Pro Ala Thr Gin Asn Ser Tyr Thr Thr Asp lie 432 1297 AGCGTTTCAAGCCGCAGTM ValGC Serc GGCACGGAGGGTAG Thr Leu Thr Thr 448 (Please read the notes on the back before filling in this page to write this page) Printed by the Consumer Cooperative of the Central Bureau of Standards, Ministry of Economic Affairs, 1345 GATCCAACCOGAGTGGATGCGCTATTTGATTCCGGCGGTCATCAGAAG 1392 449 Asp Pro Thr Arg Val Asp Ala Leu Phe Asp Ser Gly Gly His Gin Lys 464 1393 ATGmATACOGGGGCMAATCTGGATTGGAATATTCGGGGTGAATTG 1440 465 Met Leu He Pro Gly Gin Asn Leu Asp Trp Asn lie Arg Gly Glu Leu 480 1441 CM CGA GTC ACA CCG GTG AG C CGT GAA AAT AGC AGT GAC ACT GAA TGG 1488 481 Gin Arg Val Thr Pro Val Ser Arg Glu Asn Ser Ser Asp Ser Glu Trp 496 _ -387- (CNS) A4 specification (210X297 mm) 509722 A7 B7 V. Description of the invention (385) 1489 TATCGCTATAGCAGTGATGGCATGCGGCTGCTAAAAGTGAGTGMCAG 1536 497 Tyr Arg Tyr Ser Ser Asp Gly Met Arg Leu Leu Lys Val Ser Glu Gin 512 1537 CAGAOGGGCMCAGTACTCMGTACMCGGGTGACTTATCTGCOGGGA 1584 513 Gin Thr Gly Asn Ser Thr Gin Val Gin Arg Val Thr Tyr Leu Pro Gly 528 1585 mGAGCTACGGACAACTGGGGTrGCAGATAAAACAACCGMGATTTG 1632 529 Leu Glu Leu Arg Thr Thr Gly Val Ala Asp Lys Thr Thr Glu Asp Leu 544 1633 CAGGTGAnACGGTAGGTGAAGCGGGTCGCGCACAGGTAAGGGTATTG 1680 545 Gin Val lie Thr Val Gly Glu Ala Gly Arg Ala Gin Val Arg Val Leu 560 1681 TMGGAG CTCGAG CMGGAG He Asp Asn Asn Gin Val Arg 576 1729 TACAGCTACGATMTCTGCTTGGCTCCAGCCAGOTGMCTGGATAGC 1776 577 Tyr Ser Tyr Asp Asn Leu Leu Gly Ser Ser Gin Leu Glu Leu Asp Ser 592 1777 GAAGGGCAGATTCTCAGTCAGGMGAGTAT TATCOGTATGGCGGTACG 1824 593 Glu Gly Gin He Leu Ser Gin Glu Glu Tyr Tyr Pro Tyr Gly Gly Thr 608 1825 GOGATATGGGCGGCGAGAMTCAGACAGAAGCCAGCTACAAATITAn 1872 609 Ala He Trp Ala Ala Arg Asn Gin Thr Glu Ahe Ser Tung Standard Glu Ahe Ser (Please read the precautions on the back before filling out this page) 1873 CGTTACTCCGGTAAAGAGCGGGATGCCACTGGATTGTATTATTACGGC 1920 625 Arg Tyr Ser Gly Lys Glu Arg Asp Ala Thr Gly Leu Tyr Tyr Tyr Tyr Gly 640 1921 TACCGTTATTATCCCCTTGGGTGGGTCGATCOGyrGyrGC Leu Ser Ala Asp Pro 656 1969 GOGGGAACCGTGGATGGGCTGMTTTGTACCGAATGGTGAGGMTAAC 2016 657 Ala Gly Thr Val Asp Gly Leu Asn Leu Tyr Arg Met Val Arg Asn Asn 672 -388- This paper size applies the Chinese National Standard (CNS) A4 specification (210X297 mm B7 509722) V. Description of the invention (386) 2017 CCCATCACATTGACTGACCATGACGGAmGCACCGTCTCCAMTAGA 2064 673 Pro He Thr Leu Thr Asp His Asp Gly Leu Ala Pro Ser Pro Asn Arg 688 2065 MT CGA MT ACA TTT TGG TIT GCT TC A TTT TTG ITT CGT AAA CCT GAT 2112 689 Asn Arg Asn Thr Phe Trp Phe Ala Ser Phe Leu Phe Arg Lys Pro Asp 704 2113 GAGGGAATGTCCGCGTCAATGAGACGGGGACAAAAAAnGGCAGGGGGTCC AGG Serc Agg Serc Agg Serg ATC GCC 2208 721 He Ala Gly Gly lie Ala lie Gly Gly Leu Ala Ala Thr He Ala Ala 736 2209 ACGGCrGGCGCGCGTATCCCCGTCAnCTGGGGGTrGCGGCCGTAGGC 2256 737 Thr Ala Gly Ala Ala Val GlyGyCG AGG GLC ATG GLC 2TG Met Gly Tyr Asn Val Gly Ser Leu Leu Glu 768 2305 AAAGGCGGGGCAmdTGCTOGACTCGTACAGGGGAAATOGAOGm 2352 769 Lys Gly Gly Ala Leu Leu Ala Arg Leu Val Gin Gly Lys Ser Thr Leu 784 2353 GTACAGTCGGCGGClaGC AGC GGCGCGGGCGCGGGCGCGGGCGCGGCGCGGGCGCGCGCGCGCGGGCGCGCGGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGCGGC Ala Ala 800 .M Printed by the Consumers' Cooperative of the Central Bureau of Standards of the Ministry of Economic Affairs (please read the precautions on the back before filling this page) 2401 TATGGCGCACGGGC ACMGGTGTCGGTGTTGCATCAGCCGCCGGGGCG 2448 801 Tyr Gly Ala Arg Ala Gin Gly Val Gly Val Ala Ser Ala Ala Gly Ala 816 2449 GTAACAGGGGCTGTGGGATCATGGATAMTAATGCTGATGGGGGGATr 2496 817 Val Thr Gly Ala Val Gly Ser Trp lie Asn Asn Ala Asp Arg Gly lie 832 2497 GGCGGCGCTATTGGGGCCGGGAGTGCGGTAGGCACCATTGATACTATC 2544 833 Gly Gly Ala lie Gly Ala Gly Ser Ala Val Gly Thr He Asp Thr Met 848 __- 389- __ This paper size applies to Chinese National Standard (CNS) A4 specification (210X297 mm) " " 509722 A7 B7 V. Description of the invention (387) 2545 TTAGGGACTGCCTCTACCaTAGCCATGMGTCGGGGCAGCGGOGGGTGGGT 2592 849 Leu Gly Thr Ala Ser Thr Leu Thr His Glu Val Gly Ala Ala Ala Gly 864 (Please read the notes on the back before filling out this page) 2593 GGGGCGGCGGGTGGGATGATCACCGGTACGCMGGGAGTACTCGGGCA 2640 865 Gly Ala Ala Gly Gly Met lie Thr Gly Thr Gly Thr Arg Ala 880 2641 GGTATCCATGCCGGTAHGGCACCTATTATGGCTOCTGGAHGGTTTT 2688 881 Gly lie His Ala Gly He Gly Thr Tyr Tyr Gly Ser Trp He Gly Phe 896 2689 GGTmGATGTCOTAGTMCOrarGKjACATmGCGAATTACGCA 2736 8 97 Gly Leu Asp Val Ala Ser Asn Pro Ala Gly His Leu Ala Asn Tyr Ala 912 2737 GTGGGTTATGCCGCTGGTTTGGGTGCTGMATGGCTGTCMCAGAATA 2784 913 Val Gly Tyr Ala Ala Gly Leu Gly Ala Glu GTC GTCCATGGCTGTGTGTGTGTGTGTGTGTGTGTG Arg Leu Leu Gly Arg Val Val Ser Pro 944 2833 TATGCCGCCGGTTTAGCCAGACMmGTACATTTCAGTGTCGCCAGA 2880 945 Tyr Ala Ala Gly Leu Ala Arg Gin Leu Val His Phe Ser Val Ala Arg 960 2881 CCT GTCTTT GAG CCGATATTTAGTGTTC TGCGGhe GPTCGGGCGCT Leu Val Gly Gly 976 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 2929 ATTGGAACTGGCCTGCACAGAGTGATGGGAAGAGAGAGTTGGATTTCC 2976 977 lie Gly Thr Gly Leu His Arg Val Met Gly Arg Glu Ser Trp lie Ser 992 2977 AGAGOGmAGTGTGCTGCCGGTAGGAGGTGAGGTGTAGGAGTAGGCG Asp His Val Ala Gly Met 1008 3025 ATT GGT AAT CAG ATC AGA GGC AGG GTC TTG ACC ACA ACC GGG ATC GCT 3072 _-390- China National Standard (CNS) A4 specification (210X297 mm) 509722 A7 B7 V. Description of the invention (1009 IleGlyAsnGln He Arg Gly Arg Val LeuThr Thr Thr Gly He Ala 1024 3073 MTGCGATAGACTATGGCACCAGTGCTGTGGGGGCCGCACGACGAGTT 3120 10T Ser Ala Ila Ila Ala Ala Arg Arg Val 1040 3121 ΊΤΓ TCT TTG TAA 3132 1041 Phe Ser Leu End 1043 ⑵Sequence identification number 61: (i) Sequence characteristics: (A) Length: 1043 amino acids (B) Type: Amine Base acid (C) Form: Linear (ii) Molecular type: Protein (xi) Sequence description. · Sequence identification number 61 (TccC peptide) 1 Met Ser Pro Ser Glu Thr Thr Leu Tyr Thr Gin Thr Pro Thr Val Ser 16 17 Val Leu Asp Asn Arg Gly Leu Ser He Arg Asp lie Gly Phe His Arg 32 33 He Val He Gly Gly Asp Thr Asp Thr Arg Val Thr Arg His Gin Tyr 48 49 Asp Ala Arg Gly His Leu Asn Tyr Ser He Asp Pro Arg Leu Tyr Asp 64 65 Ala Lys Gin Ala Asp Asn Ser Val Lys Pro Asn Phe Val Trp Gin His 80 81 Asp Leu Ala Gly His Ala Leu Arg Thr Glu Ser Val Asp Ala Gly Arg 96 Central Standard Printed by the Bureau's Consumer Cooperative (please read the notes on the back before filling this page) 97 Thr Val Ala Leu Asn Asp lie Glu Gly Arg Ser Val Met Thr Met Asn 112 113 Ala Thr Gly Val Arg Gin Thr Arg Arg Tyr Glu Gly Asn Thr Leu Pro 128 129 Gly Arg Leu Leu Ser Val Ser Glu Gin Val Phe Asn Gin Glu Ser Ala 144 145 Lys Val Thr Glu Arg Phe He Trp Ala Gly Asn Thr Thr Ser Glu Lys 160 161 Glu Tyr Asn Leu Ser Gly Leu Cys He Arg His Tyr Asp Thr Ala Gly 176 177 Val Thr Arg Leu Met Ser Gin Ser Leu Ala Gly Ala Met Leu Ser Gin 192 _-391- This paper size applies to the Chinese National Standard (CNS) A4 size (210X297 mm) 509722 A7 B7 V. Description of the invention ('193 Ser His Gin Leu Leu Ala Glu Gly Gin Glu Ala Asn Trp Ser Gly Asp 208 209 Asp Glu Thr Val Trp Gin Gly Met Leu Ala Ser Glu Val Tyr Thr Thr 224 225 Gin Ser Thr Thr Asn Ala lie Gly Ala Leu Leu Thr Gin Thr Asp Ala 240 241 Lys Gly Asn He Gin Arg Leu Ala Tyr Asp He Ala Gly Gin Leu Lys 256 257 Gly Ser Trp Leu Thr Val Lys Gly Gin Ser Glu Gin Val He Val Lys 272 273 Ser L eu Ser Trp Ser Ala Ala Gly His Lys Leu Arg Glu Glu His Gly 288 289 Asn Gly Val Val Thr Glu Tyr Ser Tyr Glu Pro Glu Thr Gin Arg Leu 304 305 lie Gly lie Thr Thr Arg Arg Ala Glu Gly Ser Gin Ser Gly Ala Arg 320 321 Val Leu Gin Asp Leu Arg Tyr Lys Tyr Asp Pro Val Gly Asn Val lie 336 337 Ser lie His Asn Asp Ala Glu Ala Thr Arg Phe Trp Arg Asn Gin Lys 352 353 Val Glu Pro Glu Asn Arg Tyr Val Tyr Asp Ser Leu Tyr Gin Leu Met 368 369 Ser Ala Thr Gly Arg Glu Met Ala Asn lie Gly Gin Gin Ser Asn Gin 384 385 Leu Pro Ser Pro Val lie Pro Val Pro Thr Asp Asp Ser Thr Tyr Thr 400 401 Asn Tyr Leu Arg Thr Tyr Thr Tyr Asp Arg Gly Gly Asn Leu Val Gin 416 417 lie Arg His Ser Ser Pro Ala Thr Gin Asn Ser Tyr Thr Thr Asp lie 432 433 Thr Val Ser Ser Arg Ser Asn Arg Ala Val Leu Ser Thr Leu Thr Thr 448 Central Standard of the Ministry of Economy Printed by the Bureau's Consumer Cooperative (please read the notes on the back before filling this page) 449 Asp Pro Thr Arg Val Asp Ala Leu Phe Asp Ser Gly Gly His Gin Lys 464 465 Met Leu lie Pro Gly Gin Asn Leu Asp Tr p Asn lie Arg Gly Glu Leu 480 481 Gin Arg Val Thr Pro Val Ser Arg Glu Asn Ser Ser Asp Ser Glu Trp 496 497 Tyr Arg Tyr Ser Ser Asp Gly Met Arg Leu Leu Lys Val Ser Glu Gin 512 513 Gin Thr Gly Asn Ser Thr Gin Val Gin Arg Val Thr Tyr Leu Pro Gly 528 ____n This paper size applies Chinese National Standard (CNS) A4 specification (210X297 mm) 509722 A7 B7 V. Description of the invention (39i 529 Leu Glu Leu Arg Thr Thr Gly Val Ala Asp Lys Thr Thr Glu Asp Leu 544 545 Gin Val IleThr Val GlyGlu AlaGly Arg AlaGlnVal Arg Val Leu 560 561 His Trp Glu Ser Gly Lys Pro Thr Asp lie Asp Asn Asn Gin Val Arg 576 577 Tyr Ser Tyr Asp Asn Leu Leu Gin Ser G Leu Glu Leu Asp Ser 592 593 Glu Gly Gin He Leu Ser Gin Glu Glu Tyr Tyr Pro Tyr Gly Gly Thr 608 609 Ala He Trp Ala Ala Arg Asn Gin Thr Glu Ala Ser Tyr Lys Phe lie 624 625 Arg Tyr Ser Gly Lys Glu Arg Asp Ala Thr Gly Leu Tyr Tyr Tyr Gly 640 641 Tyr Arg Tyr Tyr Gin Pro Trp Val Gly Arg Trp Leu Ser Ala Asp Pro 656 657 Ala Gly Thr Val Asp Gly Leu Asn Leu Tyr Arg Met Val Arg Asn A sn 672 673 Pro He Thr Leu Thr Asp His Asp Gly Leu Ala Pro Ser Pro Asn Arg 688 689 Asn Arg Asn Thr Phe Trp Phe Ala Ser Phe Leu Phe Arg Lys Pro Asp 704 705 Glu Gly Met Ser Ala Ser Met Arg Arg Gly Gin Lys He Gly Arg Ala 720 721 He Ala Gly Gly He Ala lie Gly Gly Leu Ala Ala Thr lie Ala Ala 736 737 Thr Ala Gly Ala Ala He Pro Val lie Leu Gly Val Ala Ala Val Gly 752 753 Ala Gly He Gly Ala Leu Met Gly Tyr Asn Val Gly Ser Leu Leu Glu 768 769 Lys Gly Gly Ala Leu Leu Ala Arg Leu Val Gin Gly Lys Ser Thr Leu 784 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (Please read the notes on the back before filling this page ) 785 Val Gin Ser Ala Ala Gly Ala Ala Ala Ala Gly Ala Ser Ser Ala Ala Ala 800 801 Tyr Gly Ala Arg Ala Gin Gly Val Gly Val Ala Ser Ala Ala Gly Ala 816 817 Val Thr Gly Ala Val Gly Ser Trp He Asn Asn Ala Asp Arg Gly lie 832 833 Gly Gly Ala lie Gly Ala Gly Ser Ala Val Gly Thr lie Asp Thr Met 848 849 LeuGlyThr Ala Ser Thr Leu Thr His Glu Val Gly Ala Ala AlaGly 864 865 Gly Ala Ala Gly Gly Met He Thr G ly Thr Gin Gly Ser Thr Arg Ala 880 __ —__ n This paper is again applicable to China National Standard (CNS) A4 specification (210X297 mm) 509722 A7 B7 V. Description of the invention (39)) 881 Gly lie His Ala Gly lie Gly Thr Tyr Tyr Gly Ser Trp He Gly Phe 896 897 Gly Leu Asp Val Ala Ser Asn Pro Ala Gly His Leu Ala Asn Tyr Ala 912 913 Val Gly Tyr Ala Ala Gly Leu Gly Ala Glu Met Ala Val Asn Arg He 928 929 Met Gly Gly Gly Phe Leu Ser Arg Leu Leu Gly Arg Val Val Ser Pro 944 945 Tyr Ala Ala Gly Leu Ala Arg Gin Leu Val His Phe Ser Val Ala Arg 960 961 Pro Val Phe Glu Pro lie Phe Ser Val Leu Gly Gly Leu Val Gly Gly 976 977 lie Gly Thr Gly Leu His Arg Val Met Gly Arg Glu Ser Trp He Ser 992 993 Arg Ala Leu Ser Ala Ala Gly Ser Gly He Asp His Val Ala Gly Met 1008 1009 lie Gly Asn Gin He Arg Gly Arg Val Leu Thr Thr Thr Gly He Ala 1024 1025 Asn Ala lie Asp Tyr Gly Thr Ser Ala Val Gly Ala Ala Arg Arg Val 1040 1041 Phe Ser Leu 1043 ⑵Data of sequence identification number 62: TcaAiv (i) Sequence characteristics .. (A) Length: 5 amino acids (B) Type: Amino acid (C) Number of strands: Single strand (D) Form: Linear (ii) Molecular type: Protein (v) Fragment type: Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Internal Economics Please fill in this page again.) (Xi) Sequence description: Sequence identification number 62: TcaAiv Asn He Gly Gly Asp 1 5 ⑵Sequence identification number 63: TcaAii-syn (i) Sequence characteristics: (A) Length : 20 Amino Acids (B) Type: Amino Acids (C) Number of Units: Single Strand _____- 394- This paper size applies to China National Standard (CNS) A4 (210X 297 mm) 509722 Central Standard of the Ministry of Economic Affairs Printed by the Bureau ’s Consumer Cooperatives. V. Invention Description (39j (D) Form: Linear (ii) Molecular Type: Protein Club 0 Fragment Type: Internal (xi) Sequence Description: Sequence Identification Number 63: TcaAii-syn Cys Leu Arg Gly Asn Ser Pro Thr Asn Pro Asp Lys Asp Gly lie 1 5 10 15 Phe Ala Gin Val Ala A 7 B7 20 资料 Data of Sequence Identification Number 64: TcaAi i "syn (i) Sequence Features: (A) Length · 20 amino acids (B) type: amino acids (C) number of strands: single strand (D) morphology: linear (ii) molecular type: egg White matter (v) Fragment type: Internal (xi) Sequence description: Thick row identification number 64: TcaAiii-syn

Cys Tyr Thr Pro Asp Gin Thr Pro Ser Phe Tyr Glu Thr Ala Phe 1 5 10 15

Arg Ser Ala Asp Gly 20 资料 Data of sequence identification number 65: TcaBi_syn (i) Sequence characteristics: (A) Length: 19 amino acids (B) Type: amino acids (C) Number of shares: single strand ( D) Morphology: Linear (ii) Molecular type: Protein (v) Fragment type: Internal (xi) Sequence Xu Yuan: Sequence identification number 65: TcaBi-syn

His Gly Gin Ser Tyr Asn Asp Asn Asn Tyr Cys Asn Phe Thr Leu 1 5 10 15

Ser lie Asn Thr 19 _ _ Ming 5-_ This paper size applies to China National Standard (CNS) A4 (210X 297 mm) Γ --- ^ ------------ 'll-- ---- 0 (Please read the precautions on the back before filling out this page) 509722 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs 5. Description of the invention (39g ⑵Serial Identification Number 66: TcaBH-syn (i ) Sequence characteristics: (A) Length: 20 amino acids (B) Type: Amino acid (C) Number of strands: Single strand (D) Morphology: Linear (ii) Molecular type ·· Protein (v) Fragment type: Internal (xi) sequence description: sequence identification number 66: TcaBH-syn Cys Val Asp Pro Lys Thr Leu Gin Arg Gin Gin Ala Gly Gly Asp 1 5 10 15 Gly Thr Gly Ser Ser 20 A7 B7 ⑵Sequence identification number 67 Information of No .: TcaC_syn (i) Sequence characteristics: (A) Length: 20 amino acids (B) Type: amino acid (C) Number of strands: single strand (D) Form: linear (ii) Molecular type: protein ⑺Segment type: internal (xi) sequence description: sequence identification number 67: TcaC-syn

Cys Tyr Lys Ala Pro Gin Arg Gin Glu Asp Gly Asp Ser Asn Ala 15 10 15

Val Thr Tyr Asp Lys 20 资料 Data of sequence identification number 68: TcbAi i -syn (i) Sequence characteristics: (A) Length: 20 amino acids (B) Type: amino acids (C) Number of shares: Single strand (D) Shape: Linear (ii) Molecular type: Protein ________ -3Q6- This paper size is applicable to China National Standard (CNS) A4 specification (210X297 mm) ▼ ---- ^ ------ 0 ^ -----— 1T ------ 0 (Please read the notes on the back before filling out this page) 509722 Printed by the Consumers' Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs A7 B7 V. Invention Description (39) (V ) Fragment type: internal (xi) sequence description: sequence identification number 68: TcbAii-syn

Cys Tyr Asn Glu Asn Pro Ser Ser Glu Asp Lys Lys Trp Tyr Phe 15 10 15

Ser Ser Lys Asp Asp 20 ⑵Data of sequence identification number 69: TcbAiifSyn (i) Sequence characteristics: (A) Length: 20 amino acids (B) Type: amino acid (C) Number of shares: single strand ( D) Form: Linear (ii) Molecular type: Protein (V) Fragment type: Internal (xi) Sequence description: Sequence identification number 69: TcbAiii-syn Cys Phe Asp Ser Tyr Ser Gin Leu Tyr Glu Glu Asn He Asn Ala 15 10 15

Gly Glu Gin Arg Ala 20 ⑵Data of sequence identification number 70: TcdAi "syn (i) Sequence characteristics: (A) Length: 22 amino acids (B) Type: amino acids (C) Number of shares: single Strand (D) morphology: linear (ii) Molecular type: protein (v) fragment type: internal (xi) sequence description: sequence identification number 70: TcdAii-syn

Cys Asn Pro Asn Asn Ser Ser Asn Lys Leu Met Phe Tyr Pro Val 15 10 15

Tyr Gin Tyr Ser Gly Asn Thr 20 __- 397- This paper size applies to China National Standard (CNS) A4 specification (210 X 297 mm) .-IL ------------ IT --- --- 0 (Please read the notes on the back before filling this page) 509722 A7 B7 V. Description of the invention (39) ⑵Serial identification number 71: TcdAui-syn (i) Sequence characteristics: (A) : 20 amino acids (B) pattern: amino acid (C) strands: single strand (D) morphology · linear (ii) split sheep pattern: protein (vj fragment pattern: internal (xi) sequence description: sequence Identification number 71: TcdAiii-syn

Val Ser Gin Gly Ser Gly Ser Ala Gly Ser Gly Asn Asn Asn Leu 1 5 10 15

Ala Phe Gly Ala Gly 20 资料 Data of Sequence Identification Number 72: (i) Sequence characteristics: (A) Length: 12 amino acids (B) Type: Amino acid (C) Number of shares: Single strand (D ) Morphology: Linear (ii) Molecular type: Protein (v) Fragment type: N-terminal 列 Column I Narration: Column identification number 72: 160kDa-Hb

Met Gin Asp Ser Pro Glu Val Ala lie Thr Thr Leu 1 5 l〇 (2) Serial Identification Number No. 73: (i) Sequence characteristics: Printed by the Consumers' Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (please read the back page first) Note: Please fill in this page again.) (A) Length: 8 amino acids (B) Type: Amino acid (C) Number of strands: Single strand (D) Morphology: Linear (ii) Molecular type: Protein (v) fragment Type: N-terminal

(xi) Sequence description: Sequence identification number 73: Π0 kDa-WIR

Met Gin Arg Ser Ser Glu Val Ser 1 5 (2) Information of Serial Identification Number 74: _____: 398- _ This paper size is applicable to China National Standard (CNS) A4 specification (210 X 297 mm) 509722 A7 B7 5 Description of the invention (39i (i) Sequence characteristics: (A) Length: 12 amino acids (B) Type: Amino acid (C) Number of strands: Single strand (D) Form: Linear (ii) Molecular type: Protein (v) Fragment type-end (xi) sequence description: Sequence identification number 74: 180 kDa-H9 Met Gin Asplie Pro Glu Val Gin Leu Asn 1 5 10 资料 Sequence identification number 75: (i) Sequence characteristics: (A) Length: 12 amino acids (B) Pattern: Amino acid (C) Number of strands: Single strand (D) Morphology: Linear (ii) Molecular type: Protein (v) Fragment type: N- Terminal (xi) sequence description: Sequence identification number 75: 170 kDa-Hm (2) Met Gin Asp Ser Pro Glu Val Ser Val Thr Gin Asn 1 5 10 资料 Data of sequence identification number 76: (i) Sequence Features: (A) Length: 15 amino acids (B) Type: Printed by the Consumers' Cooperative of the Central Standards Bureau of the Ministry of Economics of the Amino Acid (Please read the notes on the back before filling this page) (C) Number of shares: Unit (D) Form: linear (ii) Molecular type: protein fragments Μ Type: N- terminal (xi) Sequence description: SEQ ID No. Identification 76: 74 kDa - H9

Ser Glu Ser Leu Phe Thr Gin Ser Leu Lys Glu Ala Arg Arg Asp 1 5 10 15 资料 Sequence ID No. 77: (i) Sequence characteristics: _ • 柳 · _ This paper standard applies to Chinese National Standard (CNS) A4 specifications (210X297 mm) 509722 A 7 B7 V. Description of the invention (39) (A) Length: 14 amino acids (B) Type: amino acids (C) Number of strands: single strand (D) Form: linear (ii) Molecular type: protein (v) fragment type: N-terminal (xi) sequence description: sequence identification number 77: 71 kDa-Hb Met Asn Leu He Glu Ala Lys Leu Gin Glu Asn Arg Asp Ala 1 5 10 (2) Information of sequence identification number 78: (i) Sequence characteristics: (A) Length: 15 amino acids (B) Type: amino acids (C) Number of strands: single strand (D) Form: linear (ii) Molecular type: protein (v) fragment type: N-terminal (xi) sequence description: sequence identification number 78: 170 kDa-H9

Met Leu Ser Thr Met Glu Lys Gin Leu Asn Glu Ser Gin Arg Asp 15 10 15 资料 Data of sequence identification number 79: (i) Sequence characteristics: (A) Length: 15 amino acids (B) Type: Amine Base acid (C) Number of shares: Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (please read the precautions on the back before filling this page) (D) Form: Linear (ii) Molecular type. Protein (v) Fragment type: N-terminal (xi) sequence description #: Sequence identification number 79: 109 kDa-Hm

Met Leu Asp He Met Glu Lys Gin Leu Asn Glu Ser Glu Arg Asp 15 10 15 资料 Data of sequence identification number 80: (i) Sequence characteristics: (A) Length: 8 amino acids (B) Type: Amine Base acid _ .400 ^ _ This paper size applies to China National Standard (CNS) A4 (21〇 ×: 297mm)

Description of the invention (39J Printed by the Consumer Cooperative of the Central Standards Bureau of the Ministry of Economic Affairs (C) Number of shares: single (D) Form: linear (ii) Molecular type: protein (v) fragment type: N-terminal (xi) sequence description \ Sequence identification number 80: 170 kDa-WX-1 Met Gin Asp Ser Arg Glu Val Ser (¾Sequence identification number 81: (i) Sequence characteristics: (A) Length: 12 amino acids ( B) Type: Amino acid (C) Number of strands: Single strand (D) Form: Linear (ii) Molecular type: Protein (v) Fragment type: N-terminal (xi) Sequence description \ Serial identification number 81: 69 kDa-H9 Leu Arg Ser Ala Xxx Ser Ala Leu Thr Thr Leu Leu 10 资料 Data of sequence identification number 82: (i) Sequence characteristics: (A) Length: 15 amino acids (B) Type: amino Acid (C) Number of strands: Single strand (D) Pattern: Linear (ii) Molecular pattern: Protein (v) Fragment pattern: N-terminal (xi) Sequence description: Sequence identification number 82: 64 kDa-HP88

Leu Lys Leu Ala Asp Asn Gly Tyr Phe Asn Glu Pro Leu Asn Val 15 10 15 资料 Data of sequence identification number 83: (i) Sequence characteristics: (A) Length: 15 amino acids (B) Type: Amine Base (C) Number of strands: Single strand (D) Form: Linear

40L This paper size applies to China National Standard (CNS) A4 (210X297 mm) (Please read the precautions on the back before filling this page)

509722 A7 B7 V. Description of the invention (39) (ii) Molecular type: protein (V) fragment type: N-terminus (xi) sequence description: sequence identification number 83: 70 kDa-NC-1

Leu Lys Leu Ala Asp Asn Ser Tyr Phe Asn Glu Pro Leu Asn 15 10 15 资料 Data of sequence identification number 84: (i) Sequence characteristics: (A) Length: 15 amino acids (B) Type: amino Acid (C) Number of strands: Single strand (D) Morphology: Linear (ii) Molecular type: Protein (v) Fragment type: N-terminus

(xi) Nose column ii: Sequence identification number 84: 60 kDa-WIR

Ser Lys Asp Glu Ser Lys Ala Asp Ser Gin Leu Val Tyr His Thr 15 10 15 (2) Information of sequence identification number 85: (i) Sequence characteristics: (A) Length: 14 amino acids (B) Type : Amino acid (C) Number of strands: Single strand (D) Form: Linear (ii) Molecular type: Protein (v) Fragment type: N-terminal (xi) ¥ Column description: Sequence identification number 85: 58 kDa -NC-1

Met Lys Lys Arg Gly Leu Thr Thr Asn Ala Gly Ala Pro Val 1 5 10 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs (please read the precautions on the back before filling this page) (2) Serial ID No. 86 Information: (i) Sequence characteristics: (A) Length: 15 amino acids (B) Type: Amino acid (C) Number of strands: Single strand (D) Form: Linear (ii) Molecular form: Protein (v) Fragment type: N-terminal (xi) i narration: Sequence identification number 86: 60 kDa-WX-12 Met Leu Asn Pro lie Val Arg Lys Phe Glu Tyr Gly Glu His Thr ____ _ -402- Applicable to this paper size China National Standard (CNS) A4 specification (210X297 mm) 509722 A7 B7

V. Description of the invention (4〇J 1 5 10 15 资料 Sequence ID No. 87: (Please read the notes on the back before filling this page) (i) Sequence characteristics: (A) Length: 15 amine groups Acid (B) Type: Amino acid (C) Number of strands: Single strand (D) Morphology: Linear (ii) Molecular type: Protein (v) Fragment type: N-terminal (xi) Sequence description: Sequence identification number 87 Size: 60 kDa-Hm

Ala Glu He Tyr Asn Lys Asp Gly Asn Lys Leu Asp Leu Tyr Gly 1 5 10 15 (2) Information of sequence identification number 88: (i) Sequence characteristics: (A) Length: 15 amino acids (B) Type: Amino acid (C) Number of strands: Single strand (D) Form: Linear (ii) Molecular type: Protein (v) Fragment type: N-terminal 6d) Sequence description ii: Sequence identification number 88: 140 kDa -Hm

Asn Leu He Glu Ala Thr Leu Glu Gin Asn Leu Arg Asp Ala 15 10 15 Printed by the Consumer Cooperatives of the Central Standards Bureau of the Ministry of Economic Affairs -403- This paper size applies to China National Standard (CNS) A4 (210X297 mm)

Claims (1)

509722 Application for Patent Scope No. 86112391 Re-examination of Patent Application for Amendment of Patent Scope Amendment Date: July 91 1 · A method for protecting a plant from insect infestation, which includes incorporating into the plant an effective amount of oral killer Bacillus protein toxin, wherein the toxin contains a polypeptide, and the polypeptide has an N-terminal amino acid sequence selected from the group consisting of: sequence identification number 1 and sequence identification number No. 2, Sequence Identification Number No. 3, Sequence Identification Number No. 4, Sequence Identification Number No. 5, Sequence Identification Number No. 6, Sequence Identification Number No. 7, Sequence Identification Number No. 8, Sequence Identification Number No. No. 9, sequence identification number 10, sequence identification number 13, sequence identification number 14, sequence identification number 15, sequence identification number 16, sequence identification number 17, sequence identification number 18 Number, sequence identification number 19, sequence identification number 20, sequence identification number 21, sequence identification number 22, sequence identification number 23, sequence identification No. 24, Sequence Identification No. 37, Sequence Identification No. 38, Sequence Identification No. 39, Sequence Identification No. 40, Sequence Identification No. 41, Sequence Identification No. 42, Sequence Identification No. No. 43, sequence identification number 62, sequence identification number 72, sequence identification number 73, sequence identification number 74, sequence identification number 75, sequence identification number 76, sequence identification number No. 77, No. 78 of serial identification, No. 79 of serial identification, No. 80 of serial identification, No. 81 of serial identification, This paper size applies to China Exclusive Standard (CNS) Α4 specification (210X297 mm) (please (Read the notes on the back before filling out this page) 509722 A8 B8 C8 D8 1 Six. Patent application scope Sequence identification number 82, Sequence identification number 83, Sequence identification number 84, Sequence identification number 85, Sequence identification number 86, Sequence identification number 87 and sequence identification number 88; or wherein the toxin has an amino acid sequence selected from the group consisting of: sequence identification number 12; sequence identification number 26; sequence identification number No. 28, sequence identification number 30, sequence identification number 32, sequence identification number 34, sequence identification number 35, sequence identification number 47, sequence identification number 49, sequence identification number No. 51, sequence identification number 53, sequence identification number 55, sequence identification number 57, sequence identification number 59, and sequence identification number 61; sequence identification number 1: Phe lie Gin Gly Tyr Ser Asp Leu Phe Gly Asn 1 5 10 Sequence Identification Number 2: Met Gin Asp Ser Pro Glu Val Ser lie Thr Thr Trp 1 5 10 Sequence Identification Number 3: Ser Glu Ser Leu Phe Thr G in Thr Leu Lys Glu Ala Arg Arg Asp Ala 1 5 10 15 Leu Val Ala (Please read the notes on the back before filling out this page)-Order |: Line 丨 This paper size applies to China National Standard (CNS) A4 specifications (210X297 Mm> 509722 A8 B8 C8 D8 VI. Patent Application Sequence Identification Number 4: Ala Ser Pro Leu Ser Thr Ser Glu Leu Thr Ser Lys Leu.Asn 1 5 10 Sequence Identification Number 5: Ala Gly Asp Thr Ala Asn lie Gly Asp 1 5 Sequence ID No. 6: Leu Gly Gly Ala Ala Thr Leu Leu Asp Leu Leu Leu Pro Gin lie 15 10 15 Sequence ID No. 7: Met Leu Ser Thr Met Glu Lys Gin Leu Asn Glu 1 5 10 Sequence ID No. 8: 4 Met Asn Leu Ala Ser Pro Leu lie Ser 1 5 Sequence ID No. 9: Met lie Asn Leu Asp lie Asn Glu Gin Asn Lys lie Met Val Val Ser 15 10 15 Sequence ID No. 10: Ala Ala Lys Asp Val Lys Phe Gly Ser Asp Ala Arg Val Lys Met Leu 15 10 15 Arg Gly Val Asn 20 _________ ^ This paper size applies the Chinese National Standard (®s) A4 size (210X297 mm) * / (Please read the notes on the back before filling this page) Order · 509722 A8 B8 C8 D8 Patent application scope Sequence identification number 13: Leu lie Gly Tyr Asn Asn Gin Phe Ser Gly Xaa Ala 1 5 l〇 Sequence identification number 14: Met Gin Asn Ser Gin Thr Phe Ser Val Gly Glu Leu 1 5 10 sequence identification number 15: Ala Gin Asp Gly Asn Gin Asp Thr Phe Phe Ser Gly Asn Thr 15 10 sequence identification number 16: Met Gin Asn Ser Leu 1 5 sequence identification number 17: Ala Phe Asn lie Asp Asp Val Ser Leu Phe 1 5 10 Sequence identification number 18: Phe lie Val Tyr Thr Ser Leu Gly Val Asn Pro Asn Asn Ser Ser Asn 15 10 15 Sequence identification number 19: lie Ser Asp Leu Val Thr Thr Ser Pro Leu Ser Glu Ala lie Gly Ser Leu 15 10 15 Gin Leu Phe lie 20 Serial Identification Number 20: This paper size applies to China National Standard (CNS) A4 (210X297 mm) (please read the back first) Please fill in this page before ordering.). Order .: Line 丨 509722 A8 B8 C8 D8 VI. Scope of patent application Met Tyr Tyr lie Gin Ala Gin Gin Leu Leu Gly Pro 1 5 10 Sequence identification number 21: Gly lie Asp Ala Val Leu Ser Met Glu Thr Gin Asn lie Gin Glu Pro 1 5 10 15 Gin Leu Gly Ala Gly Thr Tyr Val Gin Leu 20 25 Sequence Identification Number 22: lie Ser Asn Pro lie Asn lie Asn Thr Gly lie Asp Ser Ala Lys 1 5 10 15 Sequence ID No. 23: Thr Tyr Leu Thr Ser Phe Glu Gin Val Ala Asn Leu Lys 1 5 10 Sequence ID No. 24: Val Leu Gly Thr Glu Asn Val lie Ala Leu Tyr Ser Glu Asn Asn Gly 1 5 10 15 Val Gin Tyr Met Gin Ile 20 Sequence Identification Number 37: Ala Phe Asn lie Asp Val Ser Leu Phe Arg Leu Leu Lys lie Thr 1 5 10 15 Asp Hi s Asp Asn Lys Asp Gly Lys lie Lys Asn Asn Leu Lys Asn Leu 20 25 30 Ser Asn Leu Tyr lie Gly Lys Leu Leu Ala Asp lie His Gin Leu Thr This paper size applies to China National Standard (CNS) A4 size (210X297 mm) (please read the back first) Please pay attention to this page again) -5- 509722 A8 B8 C8 D8 Patent Application Range 35 40 45 lie Asp Glu Leu Asp Leu Leu Leu lie Ala Val Gly Glu Gly Lys Thr 50 55 60 Asn Leu Ser Ala lie Ser Asp Lys Gin Leu Ala T hr Leu lie Arg Lys 65 70 75 80 Leu Asn Thr lie Thr Ser Trp Leu His Thr Gin Lys Trp Ser Val Phe 85 90 95 Gin Leu Phe lie Met Thr Ser Thr Ser Tyr Asn Lys Thr Leu Thr Pro 100 105 110 Glu lie Lys Asn Leu Leu Asp Thr Val Tyr His Gly Leu Gin Gly Phe 115 120 125 Asp Lys Asp Lys Ala Asp Leu Leu His Val Met Ala Pro Tyr lie Ala 130 135 140 Ala Thr Leu Gin Leu Ser Ser Glu Asn Val Ala His Ser Val Leu Leu 145 150 155 160 Trp Ala Asp Lys Leu Gin Pro Gly Asp Gly Ala Met Thr Ala Glu Gly 165 170 175 Phe Trp Asp Trp Leu Asn Thr Lys Tyr Thr Pro Gly Ser Ser Glu Ala 180 185 190 Val Glu Thr Gin Glu His lie Val Gin Tyr Cys Gin Ala Leu Ala Gin 195 200 205 Leu Glu Met Val Tyr His Ser Thr Gly lie Asn Glu Asn Ala Phe Arg 210 215 220 Leu Phe Val Thr Lys Pro Glu Met Phe Gly Ala Ala Ala Thr Gly Ala Ala Paper Size Applicable to Chinese National Standard (CNS) A4 (210 X 297 mm) ....................... ......, ............ line. (Please read the precautions on the back before filling this page) 5097 22 A8 B8 C8 D8 Patent Application Range 225 230 235 240 Pro Ala Hi s Asp Ala Leu Ser Leu lie Met Leu Thr Arg Phe Ala Asp 245 250 255 Trp Val Asn Ala Leu Gly Glu Lys Ala Ser Ser Val Leu Ala Ala Phe 260 265 270 Glu Ala Asn Ser Leu Thr Ala Glu Gin Leu Ala Asp Ala Met Asn Leu 275 280 285 Asp Ala Asn Leu Leu Leu Gin Ala Ser lie Gin Ala Gin Asn Hi s Gin 290 295 300 His Leu Pro Pro Val Thr Pro Glu Asn Ala Phe Ser Cys Trp Thr Ser 305 310 315 320 lie Asn Thr lie Leu Gin Trp Val Asn Val Ala G.ln Gin Leu Lys Cys 325 330 335 Arg Pro Thr Gly Arg Phe Arg Phe Gly Arg Ala Gly Leu Tyr Ser lie 340 345 350 Asn Glu Arg Asp Thr Asp Leu Cys Pro Val Gly Lys Arg Gly Arg Arg 355 360 365 lie Asn Arg Arg Val Glu Phe Asn Asn Arg Leu lie Hi s Tyr Asn Ala 370 375 380 Phe Leu Asp Glu Ser Arg Ser Ala Ala Leu Ser Thr Tyr Tyr lie Arg 385 390 395 400 Gin Val Ala Lys Ala Ala Ala Ala lie Lys Ser Arg Asp Asp Leu Tyr 405 410 415 Gin Tyr Leu Leu lie Asp Asn Gin Val Ser Ala Ala lie Lys Thr Thr Paper Size Applicable to China National Standard (CNS) A4 specification (210 X 297 mm) (Please read the precautions on the back before filling this page) 509722 A8 B8 C8 D8 'Applicable patent scope 420 425 430 Arg lie Ala Glu Ala lie Ala Ser lie Gin Leu Tyr Val Asn Arg Ala 435 440 445 Leu Glu Asn Val Glu Glu Asn Ala Asn Ser Gly Val lie Ser Arg Gin 450 455 460 Phe Phe lie Asp Trp Asp Lys Tyr Asn Lys Arg Tyr Ser Thr Trp Ala 465 470 475 480 Gly Val Ser Gin Leu Val Tyr Tyr Pro Glu Asn Tyr lie Asp Pro Thr 485 490 495 Met Arg lie Gly Gin Thr Lys Met Met Asp Ala Leu Leu Gin Ser Val 500 505 510 Ser Gin Ser Gin Leu Asn Ala Asp Thr Val Glu Asp Ala Phe Met Ser 515 520 525 Tyr Leu Thr Ser Phe Glu Gin Val Ala Asn Leu Lys Val lie Ser Ala 530 535 540 Tyr His Asp Asn lie Asn Asn Asn Gin Gly Leu Thr Tyr Phe lie Gly 545 550 555 560 Leu Ser Glu Thr Asp Ala Gly Glu Tyr Tyr Trp Arg Ser Val Asp His 565 570 575 Ser Lys Phe Asn Asp Gly Lys Phe Ala Ala Asn Ala Trp Ser Glu Trp 580 585 590 His Lys lie Asp Cys Pro lie Asn Pro Tyr Lys Ser Thr lie Arg Pro 595 600 605 Val lie Tyr Lys Ser Arg Leu Tyr Leu Leu Trp Leu Glu Gin Lys Glu Paper Size Applicable to China National Standard (CNS) A4 specification (210X297 mm) (Please read the precautions on the back before filling this page) Order 丨: Line · -8- 509722 A8 B8 C8 D8 VI. Application scope of patent 610 615 620 lie Thr Lys Gin Thr Gly Asn Ser Lys Asp Gly Tyr Gin Thr Glu Thr 625 630 635 640 Asp Tyr Arg Tyr Glu Leu Lys Leu Ala His lie Arg Tyr Asp Gly Thr 645 650 655 Trp Asn Thr Pro lie Thr Phe Asp Val Asn Lys Lys lie Ser Glu Leu 660 665 670 Lys Leu Glu Lys Asn Arg Ala Pro Gly Leu Tyr Cys Ala Gly Tyr Gin 675 680 685 Gly Glu Asp Thr Leu Leu Val Met Phe Tyr Asn Gin Gin Asp Thr Leu 690 695 700 Asp Ser Tyr Lys Asn Ala Ser Met Gin Gly Gly Leu Tyr lie Phe Ala Asp 705 710 715 720 Met Ala Ser Lys Asp Met Thr Pro Glu Gin Ser Asn Val Tyr Arg Asp 725 730 735 Asn Ser Tyr Gin Gin Phe Asp Thr Asn Asn Val Arg Arg Val Asn Asn 740 745 750 Arg Tyr Ala Glu Asp Tyr Glu lie Pro Ser Ser Val Ser Ser Arg Lys 755 760 765 Asp Tyr Gly Trp Gly Asp Tyr Tyr Leu Ser Met Val Tyr Asn Gly Asp 770 775 780 lie Pro Thr lie Asn T yr Lys Ala Ala Ser Ser Asp Leu Lys lie Tyr 785 790 795 800 lie Ser Pro Lys Leu Arg lie lie His Asn Gly Tyr Glu Gly Gin Lys This paper size applies to China National Standard (CNS) A4 (210X297 mm) (Please read the notes on the back before filling this page) -9- 509722 A8 B8 C8 D8 6. Application scope of patent 805 810 815 Arg Asn Gin Cys Asn Leu Met Asn Lys Tyr Gly Lys Leu Gly Asp Lys 820 825 830 Phe lie Val Tyr Thr Ser Leu Gly Val Asn Pro Asn Asn 835 840 845 Sequence ID No. 38: Arg Tyr Tyr Asn Leu Ser Asp Glu Glu Leu Ser Gin Phe lie Gly 1 5 10 15 Sequence ID No. 39: Gly Thr Ala Thr Asp Val Ser Gly Pro Val Glu lie Asn Thr Ala 15 10 15 lie Ser Pro Ala Lys 2. Sequence identification number 40: Ala Asn Ser Leu Thr Ala Leu Phe Leu Pro Gin 1 5 10 Sequence identification number 41: Leu Arg Ser Ala Asn Thr Leu Thr Asp Leu Phe Leu Pro Gin 1 5 10 Sequence identification number 42 No .: Arg Ala Leu Glu Val Glu Arg Thr Val Ser Leu Ala Glu Val Tyr 1 5 10 15 Ala Gly Leu Glu Sequence Identification Number No. 43: This paper size applies the Chinese National Standard (CNS) A4 specification (210X297 mm). .....-........., equipment ......... Order ... .......... line. (Please read the precautions on the back before filling this page) -10- 509722 A8 B8 C8 D8 VI. Scope of patent application (Please read the precautions on the back ## Fill this first Page) lie Arg Glu Asp Tyr Pro Ala Ser Leu Gly Lys 1 5 10 Sequence identification number 62: Asn lie Gly Gly Asp 1 5 Sequence identification number 72: Met Gin Asp Ser Pro Glu Val Ala lie Thr Thr Leu 1 5 10 Sequence Identification Number 73: Met Gin Arg Ser Ser Glu Val Ser 1 5 Sequence Identification Number 74: Met Gin Asplie Pro Glu Val Gin Leu Asn 1 5 10 Sequence Identification Number 1 No. 75: Stove · Met Gin Asp Ser Pro Glu Val Ser Val Thr Gin Asn 1 5 10 Sequence ID No. 76: Ser Glu Ser Leu Phe Thr Gin Ser Leu Lys Glu Ala Arg Arg Asp 15 10 15 Sequence ID No. 77 No .: Met Asn Leu lie Glu Ala Lys Leu Gin Glu Asn Arg Asp Ala 1 5 10 Serial identification number 78: This paper size applies to China National Standard (CNS) A4 (210 X 297 mm) 509722 A8 B8 € 8 D8: 'Sixth, patent application scope Met Leu Ser Thr Met Glu Lys Gin Leu Asn Glu Ser Gin Arg Asp 1 5 10 15 Sequence identification number 79: Met Leu Asp lie Met Glu Lys Gin Leu Asn Glu Ser Glu Arg Asp 15 1 5 10 Sequence ID No. 80: Met Gin Asp Ser Arg Glu Val Ser 1 5 Sequence ID No. 81 · Leu Arg Ser Ala Xxx Ser Ala Leu Thr Thr Leu Leu 1 5 10 Sequence ID No. 82: Leu Lys Leu Ala Asp Asn Gly Tyr Phe Asn Glu Pro Leu Asn Val 1 5 10 15 Sequence ID No. 83: Leu Lys Leu Ala Asp Asn Asy Ser Tyr Phe Asn Glu Pro Leu Asn 1 5 10 Sequence ID No. 84: Ser Lys Asp Glu Ser Lys Ala Asp Ser Gin Leu Val Tyr His Thr 1 5 10 15 Sequence ID No. 85: Met Lys Lys Arg Gly Leu Thr Thr Asn Ala. Gly Ala Pro Val 1 5 10 Sequence ID No. 86: (Please read the back first Please fill in this page before ordering). Order —: Line 丨 This paper size applies to China National Standard (CNS) A4 specification (210X297 mm) 509722 A8 B8 C8 D8 VI. Patent application scope Met Leu Asn Pro lie Val Arg Lys Phe Glu Tyr Gly Glu His Thr 1 5 l〇15 Sequence ID No. 87: Ala Glu lie Tyr Asn Lys Asp Gly Asn Lys Leu Asp Leu Tyr Gly 1 5 10 15 Sequence ID No. 88: Asn Leu lie Glu Ala Thr Leu Glu Gin Asn Leu Arg Asp Ala * 1 5 10 Sequence identification number 12: Met Gin Asn Ser Leu Ser Ser Thr lie Asp Thr lie Cys Gin Lys Leu 15 10 15 Gin Leu Thr Cys Pro Ala Glu lie Ala Leu Tyr Pro Phe Asp Thr Phe 20 25 30 Arg Glu Lys Thr Arg Gly Met Val Asn Trp Gly Glu Ala Lys Arg lie 35 40 45 Tyr Glu lie Ala Gin Ala Glu Gin Asp Arg Asn Leu Leu His Glu Lys 50 55 60 Arg lie Phe Ala Tyr Ala Asn Pro Leu Leu Lys Asn Ala Val Arg Leu 65 70 75 80 This paper size is applicable to Chinese National Standard (CNS) A4 (210X297 mm) (Please read the precautions on the back before filling this page) -13- 509722 A8 B8 C8 D8 6. Application scope of patent Gly Thr Arg Gin Met Leu Gly Phe lie Gin Gly Tyr Ser Asp Leu Phe 85 90 95 Gly Asn Arg Ala Asp Asn Tyr Ala Ala Pro Gly Ser Val Ala Ser Met 100 105 110 Phe Ser Pro Ala Ala Tyr Leu Thr Glu Leu Tyr Arg Glu Ala Lys Asn 115 120 125 Leu His Asp Ser Ser Ser lie Tyr Tyr Leu Asp Lys Arg Arg Pro Asp 130 135 140 Leu Ala Ser Leu Met Leu Ser Gin Lys Asn Met Asp Glu Glu lie Ser 145 150 155 160 Thr Leu Ala Leu Ser Asn Glu Leu Cys Leu Ala Gly lie Glu Thr Lys 165 170 175 Thr Gly Lys Ser Gin Asp Glu Val Met Asp Met Leu Ser Thr Tyr Arg 180 185 190 Lea Ser Gly Glu Thr Pro Tyr His His Ala Tyr Glu Thr Val Arg Glu 195 200 205 (Please read the notes on the back before filling in this page) •, I: Line 丨 -14- This paper size applies to Chinese National Standards (CNS) A4 specifications (210X297 mm) 509722 A8 B8 C8 D8 VI. Patent application scope lie Val His Glu Arg Asp Pro Gly Phe Arg His Leu Ser Gin Ala Pro 210 215 220 lie Val Ala Ala Lys Leu Asp Pro Val Thr Leu Leu Gly lie Ser Ser 225 230 235 240 His lie Ser Pro Glu Leu Tyr Asn Leu Leu lie Glu Glu lie Pro Glu 245 250 255 Lys Asp Glu Ala Ala Leu Asp Thr Leu Tyr Lys Thr Asn Phe Gly Asp 260 265 270 Tlie Thr Ala Gin Leu Met Ser Pro Ser Tyr Leu Ala Arg Tyr Tyr 275 280 285 Gly Val Ser Pro Glu Asp lie Ala Tyr Val Thr Thr Ser Leu Ser His 290 295 300 Val Gly Tyr Ser Ser Asp lie Leu Val lie Pro Leu Val Asp Gly Val 305 310 315 320 Gly Lys Met Glu Val Val Arg Val Thr Arg Thr Pro Ser Asp Asn Tyr 325 330 335 This paper size applies to China National Standard (CNS) A4 (210X297 mm) (Please read the notes on the back before filling in (This page), ^ 丨 # · • 15- 509722 A8 B8 C8 D8 Patent Application Range Thr Ser Gin Thr Asn Tyr lie Glu Leu Tyr Pro Gin Gly Gly Asp Asn 340 345 350. Tyr Leu lie Lys Tyr Asn Leu Ser Asn Ser Phe Gly Leu Asp Asp Phe 355 360 365 Tyr Leu Gin Tyr Lys Asp Gly Ser Ala Asp Trp Thr Glu lie Ala His 370 375 380 Asn Pro Tyr Pro Asp Met Val lie Asn Gin Lys Tyr Glu Ser Gin Ala 385 390 395 400 Thr lie Lys Arg Ser Asp Ser Asp Asn lie Leu Ser lie Gly Leu Gin 405 410 415 Arg Trp His Ser Gly Ser Tyr Asn Phe Ala Ala Ala Asn Phe Lys lie% 420 425 430 Asp Gin Tyr Ser Pro Lys Ala Phe Leu Leu Lys Met Asn Lys Ala lie 435 440 445 Arg Leu Leu Lys Ala Thr Gly Leu Ser Phe Ala Thr Leu Glu Arg lie (Please read the notes on the back before filling out this page) Order —: Line 丨 450 455 460 Paper size Applicable to China National Standard (CNS) A4 specification (210 X 297 mm) -16- 509722 A8 B8 C8 D8 VI. Application scope of patent Val Asp Ser Val Asn Ser Thr Lys Ser lie Thr Val Glu Val Leu Asn 465 470 475 480 Lys Val Tyr Arg Val Lys Phe Tyr lie Asp Arg Tyr Gly lie Ser Glu 485 490 495 Glu Thr Ala Ala lie Leu Ala Asn lie Asn lie Ser Gin Gin Ala Val 500 505 510 Gly Asn Gin Leu Ser Gin Phe Glu Gin Leu Phe Asn His Pro Pro Leu 515 520 525 Asn Gly lie Arg Tyr Glu lie Ser Glu Asp Asn Ser Lys His Leu Pro 530 535 540 Asn Pro Asp Leu Asn Leu Lys Pro Asp Ser Thr Gly Asp Asp Gin Arg 545 550 555 560 560 Lys Ala Val Leu Lys Arg Ala Phe Gin Val Asn Ala Ser Glu Leu Tyr 565 570 575 Gin Met Leu Leu lie Thr Asp Arg Lys Glu Asp Gly Val lie Lys Asn 580 585 590 ___ · _ΛΊ ^ This paper standard applies to Chinese national standards ( CNS) A4 specification (210X297 public love) (Please read the precautions on the back before filling this page) 509722 A8 B8 C8 D8 Patent Application Asn Leu Glu Asn Leu Ser Asp Leu Tyr Leu Val Ser Leu Leu Ala Gin 595 600 605 lie His Asn Leu Thr lie Ala Glu Leu Asn lie Leu Leu Val lie Cys 610 615 620 Gly Tyr Gly Asp Thr Asn lie Tyr Gin lie Thr Asp Asp Asn Leu Ala 625 630 635 640 Lys lie Val Glu Thr Leu Leu Trp lie Thr Gin Trp Leu Lys Thr Gin 645 650 655 Lys Trp Thr Val Thr Asp Leu Phe Leu Met Thr Thr Thr Ala Thr Ser 660 665 670 Thr Thr Leu Thr Pro Glu lie Ser Asn Leu Thr Ala Thr Leu Ser Ser 675 680 685 Thr Leu His Gly Lys Glu Ser Leu lie Gly Glu Asp Leu Lys Arg Ala 690 695 700 Met Ala Pro Cys Phe Thr Ser Ala Leu His Leu Thr Ser Gin Glu Val 705 710 715 720 This paper size is applicable to the Chinese National Standard (CNS) A4 specification (210X297 mm) (Please read the precautions on the back before filling this page). Order-: Line 丨 -18 -509722 A8 B8 C8 D8 VI. Patent Application Range Ala Tyr Asp Leu Leu Leu Trp lie Asp Gin lie Gin Pro Ala Gin lie 725 730 735 Thr Val Asp Gly Phe Trp Glu Glu Val Gin Thr Thr Pro Thr Ser Leu 740 745, 750 Lys Val lie Thr Phe Ala Gin Val Leu Ala Gin Leu Ser Leu lie Tyr 755 760 765 Arg Arg lie Gly Leu Ser Glu Thr Glu Leu Ser Leu lie Val Thr Gin 770 775 780 Ser Ser Leu Leu Val Ala Gly Lys Ser lie Leu Asp His Gly Leu Leu 785 790 795 800 Thr Leu Met Ala Leu Glu Gly Phe His Thr Trp Val Asn Gly Leu Gly 805 810 815 Gin His Ala Ser Leu lie Leu Ala Ala Leu Lys Asp Gly Ala Leu Thr 820 825 830 Val Thr Asp Val Ala Gin Ala Met Asn Lys Glu Glu Ser Leu Leu Gin 835 840 845 ____; _ -19- This paper size applies to China National Standard (CNS) A4 (210X297 mm) (Please read the precautions on the back first Refill this page) Order-Peak 509722 A8 B8 C8 D8 Patent Application Range Met Ala Ala Asn Gin Val Glu Lys Asp Leu Thr Lys Leu Thr Ser Trp 850 855 860 Thr Gin He Asp Ala lie Leu Gin Trp Leu Gin Met Ser Ser Ala Leu 865 870 875 880 Ala Val Ser Pro Leu Asp Leu Ala Gly Met Met Ala Leu Lys Tyr Gly 885 890 895 lie Asp His Asn Tyr Ala Ala Trp Gin Ala Ala Ala Ala Ala Leu Met 900 905 910 Ala Asp His Ala Asn Gin Ala Gin Lys Lys Leu Asp Glu Thr Phe Ser 915 920 925 Lys Ala Leu Cys Asn Tyr Tyr lie Asn Ala Val Val Asp Ser Ala Ala 930 935 940 Gly Val Arg Asp Arg Asn Gly Leu Tyr Thr Tyr Leu Leu Leu lie Asp Asn 945 950 955 960 Gin Val Ser Ala Asp Val lie Thr Ser Arg lie Ala Glu Ala lie Ala 965 970 975 This paper size applies to China National Standard (CNS) A4 (210X297 mm) (Please read the note on the back first Please fill in this page again) • Order 丨 -20- 509722 A8 B8 C8 D8 VI. Patent application scope Gly lie Gin Lea Tyr Val Asn Arg Ala Leu Asn Arg Asp Glu Gly Gin 980 985 990 Leu Ala Ser Asp Val Ser Thr Arg Gin Phe Phe Thr Asp Trp Glu Arg 995 1000 1005 Tyr Asn Lys Arg Tyr Ser Thr Trp Ala Gly Val Ser Glu Leu Val Tyr 1010 1015 1020 Tyr Pro Glu Asn Tyr Val Asp Pro Thr Gin Arg lie Gly Gin Thr Lys 1025 1030 1035 1040 Met Met Asp Ala Leu Leu Gin Ser lie Asn Gin Ser Gin Leu Asn Ala 1045 1050 1055 Asp Thr Val Glu Asp Ala Phe Lys Thr Tyr Leu Thr Ser Phe Glu Gin 1060 1065 1070 Val Ala Asn Leu Lys Val lie Ser Ala Tyr His Asp Asn Val Asn Val 1075 1080 1085 Asp Gin Gly Leu Thr Tyr Phe lie Gly lie Asp Gin Ala Ala Pro Gly 1090 1095 1100 This paper is sized for China National Standard (CNS) A4 (210 X 297 mm) (Please read the notes on the back before filling out this page) -21- 509722 A8 B8 C8 D8 __ / Application scope Thr Tyr Tyr Trp Arg Ser Val Asp His Ser Lys Cys Glu Asn Gly Lys 1105 1110 1115 1120 Phe Ala Ala Asn Ala Trp Gly Glu Trp Asn Lys He Thr Cys Ala Val 1125 1130 1135 Asn Pro Trp Lys Asn lie lie Arg Pro Val Val Tyr Met Ser Arg Leu 1140 1145 1150 Tyr Leu Leu Trp Leu Glu Gin Gin Ser Lys Lys Ser Asp Asp Gly Lys 1155 1160 1165 Thr Thr lie Tyr Gin Tyr Asn Leu Lys Leu Ala His lie Arg Tyr Asp 1170 1175 1180 Gly Ser Trp Asn Thr Pro Phe Thr Phe Asp Val Thr Glu Lys Val Lys 1185 1190 1195 1200 Asn Tyr Thr Ser Ser Thr Asp Ala Ala Glu Ser Leu Gly Leu Tyr Cys 1205 1210 1215 Thr Gly Tyr Gin Gly Glu Asp Thr Leu Leu Val Met Phe Tyr Ser Met 1220 1225 1230 This paper size applies to China National Standard (CNS) A4 size (210X297 mm) (Please read the precautions on the back before filling this page) 'Order · ; Line 丨 -22- 509722 A8 B8 C8 D8 VI.Application scope Gin Ser Ser Tyr Ser Ser Tyr Thr Asp Asn Asn Ala Pro Val Thr Gly 1235 1240 1245 Leu Tyr li e Phe Ala Asp Met Ser Ser Asp Asn Met Thr Asn Ala Gin 1250 1255 1260 Ala Thr Asn Tyr Trp Asn Asn Ser Tyr Pro Gin Phe Asp Thr Val Met 1265 1270 1275 1280 Ala Asp Pro Asp Ser Asp Asn Lys Lys Val lie Thr Arg Arg Val Asn 1285 1290 1295 Asn Arg Tyr Ala Glu Asp Tyr Glu lie Pro Ser Ser Val Thr Ser Asn 1300 1305 1310 Ser Asn Tyr Ser Trp Gly Asp His Ser Leu Thr Met Leu Tyr Gly Gly 1315 1320 1325 Ser Val Pro Asn lie Thr Phe Glu Ser Ala Ala Glu Asp Leu Arg Leu 1330 1335 1340 Ser Thr Asn Met Ala Leu Ser lie lie His Asn Gly Tyr Ala Gly Thr 1345 1350 1355 1360 (Please read the notes on the back before filling this page) -23- This paper size applies to Chinese national standards (CNS> A4 size (210X297 mm) 509722 A8 B8 C8 D8 Application scope of patents Arg Arg lie Gin Cys Asn Leu Met Lys Gin Tyr Ala Ser Leu Gly Asp 1365 1370 1375 Lys Phe lie lie Tyr Asp Ser Ser Phe Asp Asp Ala Asn Arg Phe Asn 1380 1385 1390 Leu Val Pro Leu Phe Lys Phe Gly Lys Asp Glu Asn Ser Asp Asp Ser 1395 1400 1405 lie Cys lie Tyr Asn Glu Asn Pro Ser Ser Glu Asp Lys Lys Trp Tyr 1410 1415 1420 Phe Ser Ser Lys Asp Asp Asn Lys Thr Ala Asp Tyr Asn Gly Gly Thr 1425 1430 1435 1440 Gin Cys lie Asp Ala Gly Thr Ser Asn Lys Asp Phe Tyr Tyr Asn Leu 1445 1450 1455 Gin Glu lie Glu Val lie Ser Val Thr Gly Gly Tyr Trp Ser Ser Tyr 1460 1465 1470 Lys lie Ser Asn Pro lie Asn lie Asn Thr Gly lie Asp Ser Ala Lys 1475 1480 1485 (Please read the precautions on the back before filling this page) • Order ·. • Line丨 This paper is compliant with China National Standard (CNS) A4 (210 X 297 mm) -24- 509722 A8 B8 C8 D8 VI. Patent application scope Val Lys Val Thr Val Lys Ala Gly Gly Asp Asp Gin lie Phe Thr Ala 1490 1495 1500 Asp Asn Ser Thr Tyr Val Pro Gin Gin Pro Ala Pro Ser Phe Glu Glu 1505 1510 1515 1520 Met lie Tyr Gin Phe Asn Asn Leu Thr lie Asp Cys Lys Asn Leu Asn 1525 1530 1535 Phe lie Asp Asn Gin Ala His lie Glu lie Asp Phe Thr Ala Thr Ala 1540 1545 1550 Gin Asp Gly Arg Phe Leu Gly Ala Glu Thr Phe lie lie Pro Val Thr 1555 1560 1565 Lys Lys Val Leu Gly Thr Glu Asn Val lie Ala Leu Tyr Ser Glu Asn 1570 1575 1580 Asn Gly Val Gin Tyr Met Gin lie Gly Ala Tyr Arg Thr Arg Leu Asn 1585 1590 1595 1600 Thr Leu Phe Ala Gin Gin Leu Val Ser Arg Ala Asn Arg Gly lie Asp 1605 1610 1615 Applicable to paper sizes Chinese National Standard (CNS) A4 Specification (210X297 mm) (Please read the precautions on the back before filling this page) #.. Order · 509722 A8 B8 C8 D8 Patent Application Scope (Please read the precautions on the back before completing this (Page) Ala Val Leu Ser Met Glu Thr Gin Asn lie Gin Glu Pro Gin Leu Gly 1620 1625 1630 Ala Gly Thr Tyr Val Gin Leu Val Leu Asp Lys Tyr Asp Glu Ser lie 1 635 1640 1645 His Gly Thr Asn Lys Ser Phe Ala lie Glu Tyr Val Asp lie Phe Lys 1650 1655 1660 Glu Asn Asp Ser Phe Val lie Tyr Gin Gly Glu Leu Ser Glu Thr Ser 1665 1670 1675 1680 Gin Thr Val Val Lys Val Phe Leu Ser Tyr Phe lie Glu Ala Thr Gly 1685 1690 1695 Asn Lys Asn His Leu Trp Val Arg Ala Lys Tyr Gin Lys Glu Thr Thr 1700 1705 1710 Asp Lys lie Leu Phe Asp Arg Thr Asp Glu Lys Asp Pro His Gly Trp 1715 1720 1725 Phe Leu Ser Asp Asp His Lys Thr Phe Ser Gly Leu Ser Ser Ala Gin 1730 1.735 1740 This paper size applies to Chinese National Standard (CNS) A4 (210X297 mm) -26- 509722 A8 B8 C8 D8 VI. Application scope of patent Ala Leu Lys Asn Asp Ser Glu Pro Met Asp Phe Ser Gly Ala Asn Ala 1745 1750 1755 1760 Leu Tyr Phe Trp Glu Leu Phe Tyr Tyr Thr Pro Met Met Met Ala His 1765 1770 1775 Arg Leu Leu Gin Glu Gin Asn Phe Asp Ala Ala Asn His Trp Phe Arg 1780 1785 1790 Tyr Val Trp Ser Pro Ser Gly Tyr lie Val Asp Gly Lys lie Ala lie 1795 1800 1805 Tyr His Trp Asn Val Arg Pro Leu Glu Gl u Asp Thr Ser Trp Asn Ala 1810 1815 1820 Gin Gin Leu Asp Ser Thr Asp Pro Asp Ala Val Ala Gin Asp Asp Pro 1825 1830 1835 1840 Met His Tyr Lys Val Ala Thr Phe Met Ala Thr Leu Asp Leu Leu Met 1845 1850 1855 Ala Arg Gly Asp Ala Ala Tyr Arg Gin Leu Glu Arg Asp Thr Leu Ala 1860 1865 1870 __; _-27- This paper size applies to China National Standard (CNS) A4 specifications (210X297 mm) (please read the precautions on the back first) (Fill in this page) 509722 A8 B8 C8 D8 Application scope Glu Ala Lys Met Trp Tyr Thr Gin Ala Leu Asn Leu Leu Gly Asp Glu 1875 1880 1885 Pro Gin Val Met Leu Ser Thr Thr Trp Ala Asn Pro Thr Leu Gly Asn 1890 1895 1900 Ala Ala Ser Lys Thr Thr Gin Gin Val Arg Gin Gin Val Leu Thr Gin 1905 1910 1915 1920 Leu Arg Leu Asn Ser Arg Val Lys Thr Pro Leu Leu Gly Thr Ala Asn 1925 1930 1935 Ser Leu Thr Ala Leu Phe Leu Pro Gin Glu Asn Ser Lys Leu Lys Gly 1940 1945 1950 Tyr Trp Arg Thr Leu Ala Gin Arg Met Phe Asn Leu Arg His Asn Leu 1955 1960 1965 Ser lie Asp Gly Gin Pro Leu Ser Leu Pro Leu Tyr Ala Lys Pro Ala 1970 1975 1980 Asp Pro Lys Ala Leu Leu Ser Ala Ala Val Ser Ala Ser Gin Gly Gly 1985 1990 1995 2000 This paper size applies to Chinese National Standard (CNS) A4 (210 X 297 mm) ......... ...................., ............ line. (Please read the note on the back first Please fill in this page again for details) 509722 A8 B8 C8 D8 VI. Application scope of patent Ala Asp Leu Pro Lys Ala Pro Leu Thr lie His Arg Phe Pr o Gin Met 2005 2010 2015 Leu Glu Gly Ala Arg Gly Leu Val Asn Gin Leu lie Gin Phe Gly Ser 2020 2025 2030 Ser Leu Leu Gly Tyr Ser Glu Arg Gin Asp Ala Glu Ala Met Ser Gin 2035 2040 2045 Leu Leu Gin Thr Gin Ala Ser Glu Leu lie Leu Thr Ser lie Arg Met 2050 2055 2060 Gin Asp Asn Gin Leu Ala Glu Leu Asp Ser Glu Lys Thr Ala Leu Gin 2065 2070 2075 2080 Val Ser Leu Ala Gly Val Gin Gin Arg Phe Asp Ser Tyr Ser Gin Leu 2085 2090 2095 Tyr Glu Glu Asn lie Asn Ala Gly Glu Gin Arg Ala Leu Ala Leu Arg 2100 2105 2110 Ser Glu Ser Ala lie Glu Ser Gin Gly Ala Gin lie Ser Arg Met Ala 2115 2120 2125 This paper is in accordance with China National Standards (CNS) A4 specifications (210 X 297 mm) (Please read the notes on the back before filling out this page) # · 'Order. -29- 509722 A8 B8 C8 D8 Patent Application Gly Ala Gly Val Asp Met Ala Pro Asn lie Phe Gly Leu Ala Asp Gly 2130 2135 2140 Gly Met His Tyr Gly Ala lie Ala Tyr Ala lie Ala Asp Gly lie Glu 2145 2150 2155 2160 Leu Ser Ala Ser Ala Lys Met Val Asp Ala Glu Lys Val A la Gin Ser 2165 2170 2175 Glu lie Tyr Arg Arg Arg Arg Gin * Glu Trp Lys lie Gin Arg Asp Asn 2180 2185 2190 Ala Gin Ala Glu lie Asn Gin Leu Asn Ala Gin Leu Glu Ser Leu Ser 2195 2200 2205 lie Arg Arg Glu Ala Ala Glu Met Gin Lys Glu Tyr Leu Lys Thr Gin 2210 2215 2220 Gin Ala Gin Ala Gin Ala Gin Leu Thr Phe Leu Arg Ser Lys Phe Ser 2225 2230 2235 2240 Asn Gin Ala Leu Tyr Ser Trp Leu Arg Gly Arg Leu Ser Gly lie Tyr 2245 2250 2255 (Please read the precautions on the back before filling this page) Order 丨: Thread 丨 This paper size applies Chinese National Standard (CNS) A4 specification (210 X 297 mm) -30- 509722 A8 B8 C8 D8 Application scope Phe Gin Phe Tyr Asp Leu Ala Val Ser Arg Cys Leu Met Ala Glu Gin 2260 2265 2270 Ser Tyr Gin Trp Glu Ala Asn Asp Asn Ser lie Ser Phe Val Lys Pro 2275 2280 2285 Gly Ala Trp Gin Gly Thr Tyr Ala Gly Leu Leu Cys Gly Glu Ala Leu 2290 2295 2300 lie Gin Asn Leu Ala Gin Met Glu Glu Ala Tyr Leu Lys Trp Glu Ser 2305 2310 2315 2320 Arg Ala Leu Glu Val Glu Arg Thr Val Ser Leu Ala Val Val Tyr Asp 2325 2330 2335 Ser Leu Glu Gly Asn Asp Arg Phe Asn Leu Ala Glu Gin lie Pro Ala 2340 2345 2350 Leu Leu Asp Lys Gly Glu Gly Thr Ala Gly Thr Lys Glu Asn Gly Leu 2355 2360 2365 Ser Leu Ala Asn Ala lie Leu Ser Ala Ser Val Lys Leu Ser Asp Leu 2370 2375 2380 This paper size is applicable to China National Standard (CNS) A4 size (210 X 297 mm) (Please read the precautions on the back before filling this page) 4 Order_ 509722 A8 B8 C8 D8 Patent Application Range Lys Leu Gly Thr Asp Tyr Pro Asp Ser lie Val Gly Ser Asn Lys Val (Please read the notes on the back before filling this page) 2385 2390 2395 2400 Arg Arg lie Lys Gin lie Ser Val Ser Leu Pro Ala Leu Val Gly Pro 2405 2410 2415 Tyr Gin Asp Val Gin Ala Met Leu Ser Tyr Gly Gly Ser Thr Gin Leu 2420 2425 2430 Pro Lys Gly Cys Ser Ala Leu Ala Val Ser His Gly Thr Asn Asp Ser 2435 2440 2445 Gly Gin Phe Gin Leu Asp Phe Asn Asp Gly Lys Tyr Leu Pro Phe Glu 2450 2455 2460: Line 丨 Gly lie Ala Leu Asp Asp Gin Gly Thr Leu Asn Leu Gin Phe Pro Asn 2465 2470 2475 2480 Ala Thr Asp Lys Gin Lys Ala lie Leu Gin Thr Met Ser Asp lie lie 2485 2490 2495 Leu His lie Arg Tyr Thr lie Arg * 2500 2505 Serial Identification Number No. 26: This paper is in accordance with China National Standards (CNS) A4 Specifications (210X297 mm) 509722 A8 B8 C8 —D8 VI. Patent Application Range Met Ser Glu Ser Leu Phe Thr Gin Thr Leu Lys Glu Ala Arg Arg Asp 1 5 10 15 Ala Leu Val Ala His Tyr lie Ala Thr Gin Val Pro Ala Asp Leu Lys 20 25 30 Glu Ser lie Gin Thr Ala Asp Asp Leu Tyr Glu Tyr Leu Leu Leu Asp 35 40 45 Thr Lys lie Ser Asp Leu Val Thr Thr Ser Pro Leu Ser Glu Ala lie 50 55 60 Gly Ser Leu Gin Leu Phe lie His Arg Ala lie Glu Gly Tyr Asp Gly 65 70 75 80 Thr Leu Ala Asp Ser Ala Lys Pro Tyr Phe Ala Asp Glu Gin Phe Leu 85 90 95 Tyr Asn Trp Asp Ser Phe Asn His Arg Tyr Ser Thr Trp Ala Gly Lys 100 105 110 Glu Arg Leu Lys Phe Tyr Ala Gly Asp Tyr lie Asp Pro Thr Leu Arg 115 120 125 This paper size applies to China National Standard (CNS) A4 (210 X 297 mm) (Please read the back first (Notes for filling in this page) 509722 A8 B8 C8 D8 Application scope Leu Asn Lys Thr Glu lie Phe Thr Ala Phe Glu Gin Gly lie Ser Gin 130 135 140 Gly Lys Leu Lys Ser Glu Leu Val Glu Ser Lys Leu Arg Asp Tyr Leu 145 150 155 160 lie Ser Tyr Asp Thr Leu Ala Thr Leu Asp Tyr lie Thr Ala Cys Gin 165 170 175 Gly Lys Asp Asn Lys Thr lie Phe Phe lie Gly Arg Thr Gin Asn Ala 180 185 190 Pro Tyr Ala Phe Tyr Trp Arg Lys Leu Thr Leu Val Thr Asp Gly Gly 195 200 205 Lys Leu Lys Pro Asp Gin Trp Ser Glu Trp Arg Ala lie Asn Ala Gly 210 215 220 lie Ser Glu Ala Tyr Ser Gly His Val Glu Pro Phe Trp Glu Asn Asn 225 230 235 240 Lys Leu His He Arg Trp Phe Thr lie Ser Lys Glu Asp Lys lie Asp 245 250 255 (Please read the notes on the back before filling out this page), ΤΓ—: line _ This paper size applies the Chinese National Standard (CNS) A4 specification (210 X 297 mm) 509722 A8 B8 C8 D8 6. Application scope Phe Val Tyr Lys Asn lie Trp Val Met Ser Ser Asp Tyr Ser Trp Ala 260 265 270 Ser Lys Lys Lys lie Leu Glu Leu Ser Phe Thr Asp Tyr Asn Arg Val 275 280 28 5 Gly Ala Thr Gly Ser Ser Ser Pro Thr Glu Val Ala Ser Gin Tyr Gly 290 295 300 Ser Asp Ala Gin Met Asn lie Ser Asp Asly Gly Thr Val Leu lie Phe 305 310 315 320 Gin Asn Ala Gly Gly Ala Thr Pro Ser Thr Gly Val Thr Leu Cys Tyr 325 330 335 Asp Ser Gly Asn Val lie Lys Asn Leu Ser Ser Thr Gly Ser Ala Asn 340 345 350 Leu Ser Ser Lys Asp Tyr Ala Thr Thr Lys Leu Arg Met Cys His Gly 355 360 365 Gin Ser Tyr Asn Asp Asn Asn Tyr Cys Asn Phe Thr Leu Ser lie Asn 370 375 380 (Please read the notes on the back before filling this page) # · Order · This paper size applies to China National Standard (CNS) A4 (210 X 297) Love) 509722 A8 B8 C8 D8 Patent Application Range Thr lie Glu Phe Thr Ser Tyr Gly Thr Phe Ser Ser Asp Gly Lys Gin 385 390 395 400 Phe Thr Pro Pro Ser Gly Ser Ala lie Asp Leu His Leu Pro Asn Tyr 405 410 415 Val Asp Leu Asn Ala Leu Leu Asp lie Ser Leu Asp Ser Leu Leu Asn 420 425 430 Tyr Asp Val Gin Gly Gin Phe Gly Gly Ser Asn Pro Val Asp Asn Phe 435 440 445 Ser Gly Pro Tyr Gly lie Tyr Leu Trp Glu li e Phe Phe His lie Pro 450 455 460 Phe Leu Val Thr Val Arg Met Gin Thr Glu Gin Arg Tyr Glu Asp Ala 465 470 475 480 Asp Thr Trp Tyr Lys Tyr lie Phe Arg Ser Ala Gly Tyr Arg Asp Ala 485 490 495 Asn Gly Gin Leu lie Met Asp Gly Ser Lys Pro Arg Tyr Trp Asn Val 500 505 510 This paper size applies to China National Standard (CNS) A4 size (210X297 mm) ...... ... Bon ... Note for refilling this page) 509722 A8 B8 C8 D8 VI. Patent Application Range Met Pro Leu Gin Leu Asp Thr Ala Trp Asp Thr Thr Gin Pro Ala Thr 515 520 525 Thr Asp Pro Asp Val lie Ala Met Ala Asp Pro Met His Tyr Lys Leu 530 535 540 Ala lie Phe Leu His Thr Leu Asp Leu Leu lie Ala Arg Gly Asp Ser 545 550 555 560 Ala Tyr Arg Gin Leu Glu Arg Asp Thr Leu Val Glu Ala Lys Met Tyr 565 570 575 Tyr lie Gin Ala Gin Gin Leu Leu Gly Pro Arg Pro Asp lie His Thr 580 585 590 Thr Asn Thr Trp Pro Asn Pro Thr Leu Ser Lys Glu Ala Gly Ala lie 595 600 605 Ala Thr Pro Thr P he Leu Ser Ser Pro Glu Val Met Thr Phe Ala Ala 610 615 620 Trp Leu Ser Ala Gly Asp Thr Ala Asn lie Gly Asp Gly Asp Phe Leu 625 630 635 640 This paper size applies to China National Standard (CNS) A4 specifications (210X297) (Please read the precautions on the back before filling this page), OK | 509722 A8 B8 C8 D8 Patent application scope (Please read the precautions on the back before filling this page) Pro Pro Tyr Asn Asp Val Leu Leu Gly Tyr Trp Asp Lys Leu Glu Leu 645 650 655 Arg Leu Tyr Asn Leu Arg His Asn Leu Ser Leu Asp Gly Gin Pro Leu 660 665 670 Asn Leu Pro Leu Tyr Ala Thr Pro Val Asp Pro Lys Thr Leu Gin Arg 675 680 685 Gin Gin Ala Gly Gly Asp Gly Thr Gly Ser Ser Pro Ala Gly Gly Gin 690 695 700 Gly Ser Val Gin Gly Trp Arg Tyr Pro Leu Leu Val Glu Arg Ala Arg 705 710 715 720 Ser Ala Val Ser Leu Leu Thr Gin Phe Gly Asn Ser Leu Gin Thr Thr 725 730 735 Leu Glu His Gin Asp Asn Glu Lys Met Thr lie Leu Leu Gin Thr Gin 740 745 750 Gin Glu Ala lie Leu Lys His Gin His Asp lie Gin Gin Asn Asn Leu 755 760 765 paper Standards are applicable to China National Standard (CNS) A4 specifications (210 X 297 mm) 509722 A8 B8 C8 D8 Six, patent application scope Lys Gly Leu Gin His Ser Leu Thr Ala Leu Gin Ala Ser Arg Asp Gly 770 775 780 Asp Thr Leu Arg Gin Lys His Tyr Ser Asp Leu lie Asn Gly Gly Leu 785 790 795 800 Ser Ala Ala Glu lie Ala Gly Leu Thr Leu Arg Ser Thr Ala Met lie 805 810 815 Thr Asn Gly Val Ala Thr Gly Leu Leu lie Ala Gly Gly lie Ala Asn 820 825 830 Ala Val Pro Asn Val Phe Gly Leu Ala Asn Gly Gly Ser Glu Trp Gly 835 840 845 Ala Pro Leu lie Gly Ser Gly Gin Ala Thr Gin Val Gly Ala Gly lie 850 855 860 Gin Asp Gin Ser Ala Gly lie Ser Glu Val Thr Ala Gly Tyr Gin Arg 865 870 875 880 Arg Gin Glu Glu Trp Ala Leu Gin Arg Asp lie Ala Asp Asn Glu lie 885 890 895 This paper size applies to the Chinese National Standard (CNS) A4 specification (210 X 297 mm) ..... # ........ Order! : ^ 11- (Please read the notes on the back before filling this page) 509722 A8 B8 C8 D8 Patent Application Range Thr Gin Leu Asp Ala Gin lie Gin Ser Leu Gin Glu Gin lie Thr Met 900 905 910 Ala Gin Lys Gin lie Thr Leu Ser Glu Thr Glu Gin Ala Asn Ala Gin 915 920 925 Ala lie Tyr Asp Leu Gin Thr Thr Thr Arg Phe Thr Gly Gin Ala Leu Tyr 930 935 940 Asn Trp Met Ala Gly Arg Leu Ser Ala Leu Tyr Tyr Gin Met Tyr Asp 945 955 960 Ser Thr Leu Pro lie Cys Leu Gin Pro Lys Ala Ala Leu Val Gin Glu 965 970 975 Leu Gly Glu Lys Glu Ser Asp Ser Leu Phe Gin Val Pro Val Trp Asn 980 985 990 Asp Leu Trp Gin Gly Leu Leu Ala Gly Glu Gly Leu Ser Ser Glu Leu 995 1000 1005 Gin Lys Leu Asp Ala lie Trp Leu Ala Arg Gly Gly lie Gly Leu Glu 1010 1015 1020 This paper size applies to the Chinese National Standard (CNS) A4 size (210 X 297 mm) (please first Read the precautions on the back and fill in this page), TΓ—: line 丨 509722 A8 B8 C8 D8 VI. Patent application scope (please read the precautions on the back before filling out this page) Ala lie Arg Thr Val Ser Leu Asp Thr Leu Phe Gly Thr Gly Thr Leu 1025 1030 1035 1040 Ser Glu Asn lie Asn Lys Val Leu Asn Gly Glu Thr Val Ser Pro Ser 1045 1050 1055 Gly Gly Val Thr Leu Ala Leu Thr Gly Asp lie Phe Gin Ala Thr Leu 1060 1065 1070 Asp Leu Ser Gin Leu Gly Leu Asp Asn Ser Tyr Asn Leu Gly Asn Glu 1075 1080 1085 Lys Lys Arg Arg lie Lys Arg lie Ala Val Thr Leu Pro Thr Leu Leu 1090 1095 1100 Gly Pro Tyr Gin Asp Leu Glu Ala Thr Leu Val Met Gly Ala Glu lie 1105 1110 1115 1120 Ala Ala Leu Ser His Gly Val Asn Asp Gly Gly Arg Phe Val Thr Asp 1125 1130 1135 Phe Asn Asp Ser Arg Phe Leu Pro Phe Glu Gly Arg Asp Ala Thr Thr 1140 1145 1150 21 Paper size applies to Chinese National Standard (CNS) A4 specification (210 X 297 mm) 509722 A8 B8 C8 D8 Patent application scope Gly Thr Leu Glu Leu Asn lie Phe His Ala Gly Lys Glu Gly Thr Gin Π55 1160 1165 His Glu Leu Val Ala Asn Leu Ser Asp lie lie Val His Leu Asn Tyr 1170 1175 1180 lie lie Arg Asp Ala * 1185 1190 Sequence ID No. 28: Met Ser Glu Ser Leu Phe Thr Gin Thr Leu Lys Glu Ala Arg Arg Asp 15 10 15 Ala Leu Val Ala His Tyr lie Ala Thr Gin Val Pro Ala Asp Leu Lys 20 25 30 Glu Ser lie Gin Thr Ala Asp Asp Leu Tyr Glu Tyr Leu Leu Asu 35 40 45 Thr Lys lie Ser Asp Leu Val Thr Thr Ser Pro Leu Ser Glu Ala lie 50 55 60 Gly Ser Leu Gin Leu Phe lie His Arg Ala lie Glu Gly Tyr Asp Gly 65 70. 75 80 This paper size applies Chinese National Standards (CNS) M specifications (210X297 mm) 丨 …… ----------- ……, installed ........, 玎 ..... ............. (Please read the precautions on the back before filling this page) -49- 509722 A8 B8 C8 D8 Patent Application Range Thr Leu Ala Asp Ser Ala Lys Pro Tyr Phe Ala Asp Glu Gin Phe Leu 85 90 95 Tyr Asn Trp Asp Ser Phe Asn His Arg Tyr Ser Thr Trp Ala Gly Lys 100 105 110 Glu Arg Leu Lys Phe Tyr Ala Gly Asp Tyr lie Asp Pro Thr Leu Arg 115 120 125 Leu Asn Lys Thr Glu lie Phe Thr Ala Phe Glu Gin Gly lie Ser Gin 130 135 140 Gly Lys Leu Lys Ser Glu Leu Val Glu Ser Lys Leu Arg Asp Tyr Leu 145 150 155 160 lie Ser Ty r Asp Thr Leu Ala Thr Leu Asp Tyr lie Thr Ala Cys Gin 165 170 175 Gly Lys Asp Asn Lys Thr lie Phe Phe lie Gly Arg Thr Gin Asn Ala 180 185 190 Pro Tyr Ala Phe Tyr Trp Arg Lys Leu Thr Leu Val Thr Asp Gly Gly 195 200 205 This paper size applies to Chinese National Standard (CNS) A4 (210X297 mm) (Please read the precautions on the back before filling this page) 509722 A8 B8 C8 D8 6. Application scope of patents Lys Leu Lys Pro Asp Gin Trp Ser Glu Trp Arg Ala lie Asn Ala Gly 210 215 220 lie Ser Glu Ala Tyr Ser Gly His Val Glu Pro Phe Trp Glu Asn Asn 225 230 235 240 Lys Leu His lie Arg Trp Phe Thr lie Ser Lys Glu Asp Lys lie Asp 245 250 255 Phe Val Tyr Lys Asn lie Trp Val Met Ser Ser Asp Tyr Ser Trp Ala 260 265 270 Ser Lys Lys Lys lie Leu Glu Leu Ser Phe Thr Asp Tyr Asn Arg Val 275 280 285 Gly Ala Thr Gly Ser Ser Ser Pro Thr Glu Val Ala Ser Gin Tyr Gly 290 295 300 Ser Asp Ala Gin Met Asn lie Ser Asp Asp Gly Thr Val Leu lie Phe 305 310 315 320 Gin Asn Ala Gly Gly Ala Thr Pro Ser Thr Gly Val Thr Leu Cys Tyr 325 330 335 (Please read the precautions on the back before filling out this page) Order:: Line 丨 This paper size applies the Chinese National Standard (CNS) A4 specification (210 X 297 mm ) 509722 A8 B8 C8 D8 Application scope Asp Ser Gly Asn Val lie Lys Asn Leu Ser Ser Thr Gly Ser Ala Asn 340 345 350 Leu Ser Ser Lys Asp Tyr Ala Thr Thr Lys Leu Arg Met Cys His Gly 355 3 60 365 Gin Ser Tyr Asn Asp Asn Asn Tyr Gys Asn Phe Thr Leu Ser lie Asn 370 375 380 Thr lie Glu Phe Thr Ser Tyr Gly Thr Phe Ser Ser Asp Gly Lys Gin 385 390 395 400 Phe Thr Pro Pro Ser Gly Ser Ala lie Asp Leu His Leu Pro Asn Tyr 405 410 415 Val Asp Leu Asn Ala Leu Leu Asp lie Ser Leu Asp Ser Leu Leu Asn 420 425 430 Tyr Asp Val Gin Gly Gin Phe Gly Gly Ser Asn Pro Val Asp Asn Phe 435 440 445 Ser Gly Pro Tyr Gly lie Tyr Leu Trp Glu lie Phe Phe His lie Pro 450 455 460 This paper size applies to China National Standard (CNS) A4 (210 X 297 mm) (Please read the precautions on the back before filling this page) 509722 A8 B8 C8 D8 Patent application scope Phe Leu Val Thr Val Arg Met Gin Thr Glu Gin Arg Tyr Glu Asp Ala (Please read the notes on the back before filling this page) 465 470 475 480 Asp Thr Trp Tyr Lys Tyr lie Phe Arg Ser Ala Gly Tyr Arg Asp Ala 485 490 495 Asn Gly Gin Leu lie Met Asp Gly Ser Lys Pro Arg Tyr Trp Asn Val 500 505 510 Met Pro Leu Gin Leu Asp Thr Ala Trp Asp Thr Thr Gin Pro Ala Thr 515 520 525 Thr Asp Pro Asp Val lie Ala Met Ala Asp Pro Met His Tyr Lys Leu 530 535-540 Ala lie Phe Leu Hi s Thr Leu Asp Leu Leu lie Ala Arg Gly Asp Ser 545 550 555 560 Ala Tyr Arg Gin Leu Glu Arg Asp Thr Leu Val Glu Ala Lys Met Tyr 565 570 575 Tyr lie Gin Ala Gin Gin Leu Leu Gly Pro Arg Pro Asplie His Thr 580 585 590 This paper size applies to China National Standard (CNS) A4 specification (210X297 mm) -46- 509722 A8 B8 C8 D8 VI. Application scope Thr Asn Thr Trp Pro Asn Pro Thr Leu Ser Lys Glu Ala Gly Ala lie 595 600 605 Ala Thr Pro Thr Phe Leu Ser Ser Pro Glu Val Met Thr Phe Ala A la 610 615 620 Trp Leu Ser 625 Sequence Identification Number 30: Ala Gly Asp Thr Ala Asn lie Gly Asp Gly Asp Phe Leu Pro Pro Tyr 1 5 10 15 Asn Asp Val Leu Leu Gly Tyr Trp Asp Lys Leu Glu Leu Arg Leu Tyr 20 25 30 Asn Leu Arg His Asn Leu Ser Leu Asp Gly Gin Pro Leu Asn Leu Pro 35 40 45 Leu Tyr Ala Thr Pro Val Asp Pro Lys Thr Leu Gin Arg Gin Gin Ala 50 55 60 Gly Gly Asp Gly Thr Gly Ser Ser Pro Ala. Gly Gly Gin Gly Ser Val 65 70. 75 80 This paper size applies to China National Standard (CNS) A4 (210X297 mm) ..... · # ............ ..... Order ...... (Please read the notes on the back before filling this page) 47- 509722 A8 B8 C8 D8 Patent Application Scope (Please read the back first Please fill in this page again) Gin Gly Trp Arg Tyr Pro Leu Leu Val Glu Arg Ala Arg Ser Ala Val 85 90 95 Ser Leu Leu Thr Gin Phe Gly Asn Ser Leu Gin Thr Thr Leu Glu His loo 105 no Gin Asp Asn Glu Lys Met Thr lie Leu Leu Gin Thr Gin Gin Glu Ala 115 120 125 lie Leu Lys His Gin His Asp lie Gin Gin Asn Asn Leu Lys Gly Leu 130 135 140 Gin His Ser Leu Thr Ala Leu Gin Ala Ser Arg Asp Gly Asp Thr Leu 145 150 155 160 Arg Gin Lys His Tyr Ser Asp Leu lie Asn Gly Gly Leu Ser Ala Ala 165 170 175 Glu lie Ala Gly Leu Thr Leu Arg Ser Thr Ala Met lie Thr Asn Gly 180 185 190 Val Ala Thr Gly Leu Leu lie Ala Gly Gly lie Ala Asn Ala Val Pro 195 200 205 This paper size applies to China National Standard (CNS) A4 (210X297 mm) -48 -509722 A8 B8 C8 D8 Patent Application Asn Val Phe Gly Leu Ala Asn Gly Gly Ser Glu Trp Gly Ala Pro Leu 210 215 220 lie Gly Ser Gly Gin Ala Thr Gin Val Gly Ala Gly lie Gin AspGln 225 230 235 240 Ser Ala Gly lie Ser Glu Val Thr Ala Gly Tyr Gin Arg Arg Gin Glu 245 250 255 Glu Trp Ala Leu Gin Arg Asp lie Ala Asp Asn Glu lie Thr Gin Leu 260 265 270 Asp Ala Gin lie Gin Ser Leu Gin Glu Gin lie Thr Met Ala Gin Lys 275 280 285 Gin lie Thr Leu Ser Glu Thr Glu Gin Ala Asn Ala Gin Ala lie Tyr 290 295 300 Asp Leu Gin Thr Thr Arg Phe Thr Gly Gin Ala Leu Tyr Asn Trp Met 305 310 31 5 320 Ala Gly Arg Leu Ser Ala Leu Tyr Tyr Gin Met Tyr Asp Ser Thr Leu 325 330 335 This paper size applies to China National Standard (CNS) A4 specifications (210X297 mm) (Please read the precautions on the back before filling this page ) 509722 A8 B8 C8 D8 Application scope Pro lie Cys Leu Gin Pro Lys Ala Ala Leu Val Gin Glu Leu Gly Glu 340 345 350 Lys Glu Ser Asp Ser Leu Phe Gin Val Pro Val Trp Asn Asp Leu Trp 355 360 365 Gin Gly Leu Leu Ala Gly Glu Gly Leu Ser Ser Glu Leu Gin Lys Leu 370 375 380 Asp Ala lie Trp Leu Ala Arg Gly Gly lie Gly Leu Glu Ala lie Arg 385 390 395 400 Thr Val Ser Leu Asp Thr Leu Phe Gly Thr Gly Thr Leu Ser Glu Asn 405 410 415 lie Asn Lys Val Leu Asn Gly Glu Thr Val Ser Pro Ser Gly Gly Val 420 425 430 Thr Leu Ala Leu Thr Gly Asp lie Phe Gin Ala Thr Leu Asp Leu Ser 435 440 445 Gin Leu Gly Leu Asp Asn Ser Tyr Asn Leu Gly Asn Glu Lys Lys Arg 450 455 460 (Please read the precautions on the back before filling this page) •, tr—: line 丨 -SO- This paper size applies to China National Standard (CNS) A4 (210X297 mm) ) 509722 A8 B8 C8 D8 VI. Application scope Arg lie Lys Arg lie Ala Val Thr Leu Pro Thr Leu Leu Gly Pro Tyr 465 470 475 480 Gin Asp Leu Glu Ala Thr Leu Val Met Gly Ala Glu l ie Ala Ala Leu 485 490 495 Ser His Gly Val Asn Asp Gly Gly Arg Phe Val Thr Asp Phe Asn Asp 500 505 510 Ser Arg Phe Leu Pro Phe Glu Gly Arg Asp Ala Thr Thr Gly Thr Leu 515 520 525 Glu Leu Asn lie Phe His Ala Gly Lys Glu Gly Thr Gin His Glu Leu 530 535 540 Val Ala Asn Leu Ser .Asp lie lie Val His Leu Asn Tyr lie lie Arg 545 550 555 560 Asp Ala * Sequence ID No. 32: Met Gin Asp Ser Pro Glu Val Ser lie Thr Thr Leu Ser Leu Pro Lys 1 5 10 15 Gly Gly Gly Gly Ala lie Asn Gly Met Gly Glu Ala Leu Asn Ala Ala Gly _____; _-51- This paper size applies Chinese National Standard (CNS) A4 specifications ( 210X297 mm) (Please read the notes on the back before filling this page) 509722 A8 B8 C8 D8 patent application scope 20 25 30 Pro Asp Gly Met Ala Ser Leu Ser Leu Pro Leu Pro Leu Ser Thr Gly 35 40 45 Arg Gly Thr Ala Pro Gly Leu Ser Leu lie Tyr Ser Asn Ser Ala Gly 50 55 60 Asn Gly Pro Phe Gly lie Gly Trp Gin Cys Gly Val Met Ser lie Ser 65 70 75 80 Arg Arg Thr Gin His Gly lie Pro Gin Tyr Gly Asn Asp Asp Thr Phe 85 90 95 Leu Ser Pro Gin Gly Glu Val Met Asn lie Ala Leu Asn Asp Gin Gly 100 105 110 Gin Pro Asp lie Arg Gin Asp Val Lys Thr Leu Gin Gly Val Thr Leu 115 120 125 Pro lie Ser Tyr Thr Val Thr Arg Tyr Gin Ala Arg Gin lie Leu Asp 130 135 140 Phe Ser Lys lie Glu Tyr Trp Gin Pro Ala Ser Gly Gin Glu Gly Arg This paper is compatible with Chinese National Standard (CNS) A4 (210 X 297 mm) (Please read the precautions on the back before filling this page) •, # J ·: Line — -52- 509722 A8 B8 C8 D8 Patent Application Range 145 150 155 160 (Please read the notes on the back before filling this page) Ala Phe Trp Leu lie Ser Thr Pro Asp Gly His Leu His lie Leu Gly 165 170 175 Lys T hr Ala Gin Ala Cys Leu Ala Asn Pro Gin Asn Asp Gin Gin lie 180 185 190 Ala Gin Trp Leu Leu Glu Glu Thr Val Thr Pro Ala Gly Glu His Val 195 200 205 Ser Tyr Gin Tyr Arg Ala Glu Asp Glu Ala His Cys Asp Asp Asn Glu 210 215 220 Lys Thr Ala His Pro Asn Val Thr Ala Gin Arg Tyr Leu Val Gin Val 225 230 235 240 Asn Tyr Gly Asn lie Lys Pro Gin Ala Ser Leu Phe Val Leu Asp Asn 245 250 255 Ala Pro Pro Ala Pro Glu Glu Trp Leu Phe His Leu Val Phe Asp His 260 265 270 Gly Glu Arg Asp Thr Ser Leu His Thr Val Pro Thr Trp Asp Ala Gly This paper size applies to China National Standard (CNS) A4 (210 X 297 cm)- 53- 509722 A8 B8 C8 D8 Patent Application Range 275 280 285 (Please read the notes on the back before filling this page) Thr Ala Gin Trp Ser Val Arg Pro Asp lie Phe Ser Arg Tyr Glu Tyr 290 295 300 Gly Phe Glu Val Arg Thr Arg Arg Leu Cys Gin Val Leu Met Phe 305 310 315 320 His Arg Thr Ala Leu Met Ala Gly Glu Ala Ser Thr Asn Asp Ala Pro 325 330 335 Glu Leu Val Gly Arg Leu lie Leu Glu Tyr Asp Lys Asn Ala Ser Val 340 345 350 Thr Thr Leu lie Thr lie Arg Gin Leu Ser His Glu Ser Asp Gly Arg 355 360 365 Pro Val Thr Gin Pro Pro Leu Glu Leu Ala Trp Gin Arg Phe Asp Leu 370 375 380 Glu Lys lie Pro Thr Trp Gin Arg Phe Asp Ala Leu Asp Asn Phe Asn 385 390 395 400 Ser Gin Gin Arg Tyr Gin Leu Val Asp Leu Arg Gly Glu Gly Leu Pro This paper size applies to China National Standard (CNS) A4 (210X297 mm)- 54- 509722 405 410 A8 B8 C8 D8 Application scope 415 Gly Met Leu Tyr Gin Asp Arg Gly Ala Trp Trp Tyr Lys Ala Pro Gin 420 425 430 Arg Gin Glu Asp Gly Asp Ser Asn Ala Val Thr Tyr Asp Lys lie Ala 435 440 445 Pro Leu Pro Thr Leu Pro Asn Leu Gin Asp Asn Ala Ser Leu Met Asp 450 455 460 lie Asn Gly Asp Gly Gin Leu Asp Trp Val Val Thr Ala Ser Gly lie 465 470 475 480 Arg Gly Tyr Hi s Ser Gin Gin Pro Asp Gly Lys Trp Thr His Phe Thr 485 490 495 Pro lie Asn Ala Leu Pro Val Glu Tyr Phe His Pro Ser lie Gin Phe 500 505 510 Ala Asp Leu Thr Gly Ala Gly Leu Ser Asp Leu Val Leu lie Gly Pro 515 520 525 Lys Ser Val Arg Leu Tyr Ala Asn Gin Arg Asn Gly Trp Arg Lys Gly This paper size applies to China National Standard (CNS) A4 specifications (210X297 mm) (Please read the precautions on the back before filling this page ) 509722 A8 B8 C8 D8 VI. Application for Patent Range 530 535 540 (Please read the notes on the back before filling this page) Glu Asp Val Pro Gin Ser Thr Gly lie Thr Leu Pro Val Thr Gly Thr 545 550 555 560 Asp Ala Arg Lys Leu Val Ala P * he Ser Asp Met Leu Gly Ser Gly Gin 565 570 575 Gin His Leu Val Glu lie Lys Gly Asn Arg Val Thr Cys Trp Pro Asn 580 585 590 Leu Gly His Gly Arg Phe Gly Gin Pro Leu Thr Leu Ser Gly Phe Ser 595 600 605 Gin Pro Glu Asn Ser Phe Asn Pro Glu Arg Leu Phe Leu Ala Asp lie 610 615 620: Line 丨 Asp Gly Ser Gly Thr Thr Asp Leu lie Tyr Ala Gin Ser Gly Ser Leu 625 630 635 640 Leu lie Tyr Leu Asn Gin Ser Gly Asn Gin Phe Asp Ala Pro Leu Thr 645 650 655 Leu Ala Leu Pro Glu Gly Val Gin Phe Asp Asn Thr Cys Gin Leu Gin This paper size applies to China National Standard (CNS) A4 (210X297 mm)- 56- 509722 665 670 A8 B8 C8 D8 Patent Application Range 660 Val Ala Asp lie Gin Gly Leu Gly lie Ala Ser Leu lie Leu Thr Val 675 680 685 Pro Hi s lie Ala Pro Hi s His Trp Arg Cys Asp Leu Ser Leu Thr Lys 690 695 700 Pro Trp Leu Leu Asu Val Met Asn Asn Asn Arg Gly Ala His His Thr 705 710 715 720 Leu His Tyr Arg Ser Ser Ala Gin Phe Trp Leu Asp G1u Lys Leu Gin 725 730 735 Leu Thr Lys Ala Gly Lys Ser Pro Ala Cys Tyr Leu Pro Phe Pro Met 740 745 750 Hi s Leu Leu Trp Tyr Thr Glu lie Gin Asp Glu lie Ser Gly Asn Arg 755 760 765 Leu Thr Ser Glu Val Asn Tyr Ser His Gly Val Trp Asp Gly Lys Glu 770 775 780 Arg Glu Phe Arg Gly Phe Gly Cys lie Lys Gin Thr Asp Thr Thr Thr This paper size applies to China National Standard (CNS) A4 specifications (210X297 mm) (Please read the precautions on the back before filling in this page) -57- 509722 A8 B8 C8 D8 Patent Application Range 785 790 795 800 Phe Ser His Gly Thr Ala.Pro Glu Gin Ala Ala Pro Ser Leu Ser lie 805 810 815 Ser Trp Phe Ala Thr Gly Met Asp Glu Val Asp Ser Gin Leu Ala Thr 820 825 830 Glu Tyr Trp Gin Ala Asp Thr Gin Ala Tyr Ser Gly Phe Glu Thr Arg 835 840 845 Tyr Thr Val Trp Asp His Thr Asn Gin Thr Asp Gin Ala Phe Thr Pro 850 855 860 Asn Glu Thr Gin Arg Asn Trp Leu Thr Arg Ala Leu Lys Gly Gin Leu 865 870 875 880 Leu Arg Thr Glu Leu Tyr Gly Leu Asp Gly Thr Asp Lys Gin Thr Val 885 890 895 Pro Tyr Thr Val Ser Glu Ser Arg Tyr Gin Val Arg Ser lie Pro Val 900 905 910 Asn Lys Glu Thr Glu Leu Ser Ala Trp Val Thr Ala lie Glu Asn Arg (Please read the notes on the back before filling this page) • Order —: Line 丨 -58- This paper size applies to China National Standard (CNS) A4 specifications (210X297 mm) 509722 A8 B8 C8 D8 Application scope of patent 915 920 925 Ser Tyr His Tyr Glu Arg lie lie Thr Asp Pro Gin Phe Ser Gin Ser 930 935 * 940 lie Lys Leu Gin His Asp lie Phe Gl y Gin Ser Leu Gin Ser Val Asp 945 950 955 960 lie Ala Trp Pro Arg Arg Glu Lys Pro Ala Val Asn Pro Tyr Pro Pro 965 970 975 Thr Leu Pro Glu Thr Leu Phe Asp Ser Ser Tyr Asp Asp Gin Gin Gin 980 985 990 Leu Leu Arg Leu Val Arg Gin Lys Asn Ser Trp His Hi s Leu Thr Asp 995 1000 1005 Gly Glu Asn Trp Arg Leu Gly Leu Pro Asn Ala Gin Arg Arg Asp Val 1010 1015 1020 Tyr Thr Tyr Asp Arg Ser Lys lie Pro Thr Glu Gly lie Ser Leu Glu 1025 1030 1035 1040 lie Leu Leu Lys Asp Asp Gly Leu Leu Ala Asp Glu Lys Ala Ala Val -59- This paper size applies to China National Standard (CNS) A4 size (210 X 297 mm) (please first Read the notes on the back and fill out this page) # · 509722 A8 B8 C8 D8, patent application range 1045 1050 1055 Tyr Leu Gly Gin Gin Gin Thr Phe Tyr Thr Ala Gly Gin Ala Glu Val 1060 1065 1070 Thr Leu Glu Lys Pro Thr Leu Gin Ala Leu Val Ala Phe Gin Glu Thr 1075 1080 1085 Ala Met Met Asp Asp Thr Ser Leu Gin Ala Tyr Glu Gly Val lie Glu 1090 1095 1100 Glu Gin Glu Leu Asn Thr Ala Leu Thr Gin Ala Gly Tyr Gin Gin Val 1105 1110 1115 1120 Ala Arg Leu Phe Asn Thr Arg Ser Glu Ser Pro Val Trp Ala Ala Arg 1125 1130 1135 Gin Gly Tyr Thr Asp Tyr Gly Asp Ala Ala Gin Phe Trp Arg Pro Gin 1140 1145 1150 Ala Gin Arg Asn Ser Leu Leu Thr Gly Lys Thr Thr Leu Thr Trp Asp 1155 1160 1165 Thr His His Cys Val lie lie Gin Thr Gin Asp Ala Ala Gly Leu Thr (Please read the notes on the back before filling this page) Order: Line 丨-60- This paper size applies to Chinese national standard () A4 size (210X297 public love *) 509722 1170 1175 A8 B8 C8 D8 VI. Application scope of patent 1180 Thr Gin Ala His Tyr Asp Tyr Arg Phe Leu Thr Pro Val Gin Leu Thr 1185 1190 1195 1200 Asp lie Asn Asp Asn Gin His Me Val Thr Leu Asp Ala Leu Gly Arg 1205 1210 1215 Val Thr Thr Ser Arg Phe Trp Gly Thr Glu Ala Gly Gin Ala Ala Gly 1220 1225 1230 Tyr Ser Asn Gin Pro Phe Thr Pro Pro Asp Ser Val Asp Lys Ala Leu 1235 1240 1245 Ala Leu Thr Gly Ala Leu Pro Val Ala Gin Cys Leu Val Tyr Ala Val 1250 1255 1260 Asp Ser Trp Met Pro Ser Leu Ser Leu Ser Gin Leu Ser Gin Ser Gin 1265 1270 1275 1280 Glu Glu Ala Glu Ala Leu Trp Ala Gin Leu Arg Ala Ala His Met lie 1285 1290 1295 Thr Glu Asp Gly Lys Val Cys Ala Leu Ser Gly Lys Arg Gly Thr Ser -61- This paper size applies to China National Standard (CNS) A4 (210X297 mm) (Please read the precautions on the back before filling this page) 509722 A8 B8 C8 D8 patent application scope 1300 1305 1310 (Please read the notes on the back before filling this page) Hi s Gin Asn Leu Thr lie. Gin Leu lie Ser Leu Leu Ala Se r lie Pro 1315 1320 1325 Arg Leu Pro Pro His Val Leu Gly lie Thr Thr Asp Arg Tyr Asp Ser 1330 1335 1340 Asp Pro Gin Gin Gin His Gin Gin Thr Val Ser Phe Ser Asp Gly Phe 1345 1350 1355 1355 Gly Arg Leu Leu Gin Ser Ser Ala Arg Hi s Glu Ser Gly Asp Ala Trp 1365 1370 1375 Gin Arg Lys Glu Asp Gly Gly Leu Val Val Asp Ala Asn Gly Val Leu 1380 1385 1390 Val Ser Ala Pro Thr Asp Thr Arg Trp Ala Val Ser Gly Arg Thr Glu 1395 1400 1405 Tyr Asp Asp Lys Gly Gin Pro Val Arg Thr Tyr Gin Pro Tyr Phe Leu 1410 1415 1420 Asn Asp Trp Arg Tyr Val Ser Asp Asp Ser Ala Arg Asp Asp Leu Phe -62- This paper size applies to China National Standard (CNS) A4 (210 X 297 mm) 509722 A8 B8 C8 D8 Application scope 1425 1430 1435 1440 Ala Asp Thr His Leu Tyr Asp Pro Leu Gly Arg Glu Tyr Lys Val lie 1445 1450 1455 Thr Ala Lys Lys Tyr Leu Arg Glu Lys Leu Tyr Thr Pro Trp Phe lie 1460 < 1465 1470 Val Ser Glu Asp Glu Asn Asp Thr Ala Ser Arg Thr Pro * 1475 1480 1485 Sequence ID No. 34: Met Val Thr Val Met Gin Asn Lys lie Ser Phe Leu Ser Gly Thr Ser 15 10 15 Glu Gin Pro Leu Leu Asp Ala Gly Tyr Gin Asn Val Phe Asp lie Ala 20 25 30 Ser lie Ser Arg Ala Thr Phe Val Gin Ser Val Pro Thr Leu Pro Val 35 40 45 Lys Glu Ala His Thr Val Tyr Arg Gin Ala Arg Gin Arg Ala Glu Asn 50 55 60 Leu Lys Ser Leu Tyr Arg Ala Trp Gin Leu Arg Gin Glu Pro Val lie -63- (Please read the notes on the back before filling this page) This paper is compliant with the Chinese National Standard (CNS) A4 specification (210X297 mm) 509722 A8 B8 C8 D8 Patent application scope 65 75 Lys Gly Leu Ala Lys Leu Asn Leu Gin Ser Asn Val Ser Val Leu Gin 85 90 95 Asp Ala Leu Val Glu Asn lie Gly Gly Asp Gly Asp Phe Ser Asp Leu 100 105 110 Met Asn Arg Ala Ser Gin Tyr Ala Asp Ala Ala Ser lie Gin Ser Leu 115 120 125 Phe Ser Pro Gly Arg Tyr Ala Ser Ala Leu Tyr Arg Val Ala Lys Asp 130 135 140 Leu His Lys Ser Asp Ser Ser Leu His lie Asp Asn Arg Arg Ala Asp 145 150 155 160 Leu Lys Asp Leu lie Leu Ser Glu Thr Thr Met Asn Lys Glu Val Thr 165 170 175 Ser Leu Asp lie Leu Leu Asp Val Leu Gin Lys Gly Gly Lys Asp lie 180 185 190 Thr Glu Leu Ser Gly Ala Phe Phe Pro Met Thr Leu Pro Tyr Asp Asp (Please read the notes on the back before filling out this page) • Order 丨: Line 丨 -64- This paper size applies to Chinese National Standard (CNS) A4 (210 X 297 mm) 509722 195 205 A8 B8 C8 D8 Patent application scope 200 His Leu Ser Gin lie Asp Ser Ala Leu Ser Ala Gin Ala Arg Thr Leu 210 215 220 Asn Gly Val Trp Asn Thr Leu Thr Asp Thr Thr Ala Gla Ala Val Ser 225 230 235 240 Glu Gin Thr Ser Asn Thr Asn Thr Arg Lys Leu Phe Ala Ala Gin Asp 245 250 255 Gly Asn Gin Asp Thr Phe Phe Ser Gly Asn Thr Phe Tyr Phe Lys Ala 260 265 270 Val Gly Phe Ser Gly Gin Pro Met Val Tyr Leu Ser Gin Tyr Thr Ser 275 280 285 Gly Asn Gly lie Val Gly Ala Gin Leu lie Ala Gly Asn Pro Asp Gin 290 295 300 Ala Ala Ala Ala lie Val Ala Pro Leu Lys Leu Thr Trp Ser Met Ala 305 310 315 320 Lys Gin Cys Tyr Tyr Leu Val Ala Pro Asp Gly Thr Thr Met Gly Asp -65- This paper is in accordance with China National Standards (CNS) A4 specification (210X297 mm) (Please read the precautions on the back before filling this page) 509722 A8 B8 C8 D8 patent application scope 325 330 335 _ Gly Asn Val Leu Thr Gly Cys Phe Leu Arg Gly Asn Ser Pro Thr Asn 340 345 350 Pro Asp Lys Asp Gly lie Phe Ala Gin Val Ala Asn Lys Ser Gly Ser 355 360 365 Thr Gin Pro Leu Pro Ser Phe His Leu Pro Val Thr Leu Glu His Ser 370 375 380 Glu Asn Lys Asp Gin Tyr Tyr Leu Lys Thr Glu Gin Gly Tyr lie Thr 385 390 395 400 Val Asp Ser Ser Gly Gin Ser Asn Trp Lys Asn Ala Leu Val lie Asn 405 410 415 Gly Thr Lys Asp Lys Gly Leu Leu Leu Thr Phe Cys Ser Asp Ser Ser 420 425 430 Gly Thr Pro Thr Asn Pro Asp Asp Val He Pro Pro Ala lie Asn Asp 435 440 445 lie Pro Ser Pro Pro Ala Arg Glu Thr Leu Ser Leu Thr Pro Val Ser ---------------------- install ............... ... Order --------------- Line · (Please read the precautions on the back before filling out this page) -66- This paper size applies to China National Standard (CNS) M Specification U10 X 297 mm) 509722 A8 B8 C8 D8 Patent Application Range 455 Tyr Gin Leu Met Thr AsnPro Ala Pro Thr Glu Asp Asp lie Thr Asn 465 470 475 480 Hi s Tyr Gly Phe Asn Gly Ala Ser Leu Arg Ala Ser Pro Leu Ser Thr 485 490 W4 * 495 Ser Glu Leu Thr Ser Lys Leu Asn Ser lie Asp Thr Phe Cys Glu Lys 500 505 510 Thr Arg Leu Ser Phe Asn Gin Leu Met Asp Leu Thr Ala Gin Gin Ser 515 520 525 Tyr Ser Gin Ser Ser lie Asp Ala Lys Ala Ala Ser Arg Tyr Val Arg 530 535 540 Phe Gly Glu Thr Thr Pro Thr Arg Val Asn Val Tyr Gly Ala Ala Tyr 545 550 555 560 Leu Asn Ser Thr Leu Ala Asp Ala Ala Asp Gly Gin Tyr Leu Trp lie 565 570 575 Gin Thr Asp Gly Lys Ser Leu Asn Phe Thr Asp Asp Thr Val Val Ala -67- This paper size applies the Chinese National Standard (CNS) A4 as the grid ( 210 X 297 mm) (Please read the notes on the back before filling this page) 460 450 509722 A8 B8 C8 D8 Patent Application Range 580 585 590 Leu Ala Gly Arg Ala Glu Lys Leu Val Arg Leu Ser Ser Gin Thr Gly 595 600 605 Leu Ser Phe Glu Glu Leu Asp Tip Leu lie Ala Asn Ala Ser Arg Ser 610 615 620 Val Pro Asp His His Asp Lys lie Val Leu Asp Lys Pro Val Leu Glu 625 630 635 640 Ala Leu Ala Glu Tyr Val Ser Leu Lys Gin Arg Tyr Gly Leu Asp Ala 645 650 655 Asn Thr Phe Ala Thr Phe lie Ser Ala Val Asn Pro Tyr Thr Pro Asp 660 665 670 Gin Thr Pro Ser Phe Tyr Glu Thr Ala Phe Arg Ser Ala Asp Gly Asn 675 680 685 His Val lie Ala Leu Gly Thr Glu Val Lys Tyr Ala Glu Asn Glu Gin 690 695 700 Asp Glu Leu Ala Ala lie Cys Cys Lys Ala Leu Gly Val Thr Ser Asp (Please read the precautions on the back before filling out this page), order:: -68- This paper size applies to China National Standard (CNS) A4 specifications (210 X 297 mm) 509722 A8 B8 C8 D8 6. Application scope 705 710 715 720 Glu Leu Leu Arg lie Gly Arg Tyr Cys Phe Gly Asn Ala Gly Ser Phe 725 730 735 Thr Leu Asp Glu Tyr Thr Al a Ser Gin Leu Tyr Arg Phe Gly Ala lie 740 745 750 Pro Arg Leu Phe Gly Leu Thr Phe Ala Gin Ala Glu lie Leu Trp Arg 755 760 765 Leu Met Glu Gly Gly Lys Asp lie Leu Leu Gin Gin Leu Gly Gin Ala 770 775 780 Lys Ser Leu Gin Pro Leu Ala lie Leu Arg Arg Thr Glu Gin Val Leu 785 790 795 800 Asp Trp Met Ser Ser Val Asn Leu Ser Leu Thr Tyr Leu Gin Gly Met 805 810 815 Val Ser Thr Gin Trp Ser Gly Thr Ala Thr Ala Glu Met Phe Asn Phe 820 825 830 Leu Glu Asn Val Cys Asp Ser Val Asn Ser Gin Ala Ala Thr Lys Glu (Please read the notes on the back before filling this page) This paper size is in accordance with Chinese National Standard (CNS) A4 (210X297 mm) -69- 509722 A8 B8 C8 D8 6. Application scope 835 840 845 Thr Met Asp Ser Ala Leu-Gin Gin Lys Val Leu Arg Ala Leu Ser Ala 850 855 860 Gly Phe Gly lie Lys Ser Asn Val Met Gly lie Val Thr Phe Trp Leu 865 870 875 880 Glu Lys lie Thr lie Gly Ser Asp Asn Pro Phe Thr Leu Ala Asn Tyr 885 890 895 Trp Hi s Asp 11e Gin Thr Leu Phe Ser His Asp Asn Ala Thr Leu Glu 900 905 910 Ser Leu Gin Thr Asp Thr Ser Leu Val lie Ala Thr Gin Gin Leu Ser 915 920 925 Gin Leu Val Leu lie Val Lys Trp Leu Ser Leu Thr Glu Gin Asp Leu 930 935 940 Gin Leu Leu Thr Thr Tyr Pro Glu Arg Leu lie Asn Gly lie Thr Asn 945 950 955 960 Val Pro Val Pro Asn Pro Glu Leu Leu Leu Thr Leu Ser Arg Phe Lys .............. ............. Installed ........ tr .................. line · ( Please read the notes on the back before filling this page) -70- This paper size applies to China National Standard (CNS) A4 (210X 297 mm) 975509722 A8 B8 C8 D8 Range of interest 965 970 Gin Trp Glu Thr Gin Val Thr Val Ser Arg Asp Glu Ala Met Arg Cys 980 985 990 Phe Asp Gin Leu Asn Ala Asn Asp Met Thr Thr Glu Asn Ala Gly Ser 995 1000 1005 Leu lie Ala Thr Leu Tyr Glu Met Asp Lys Gly Thr Gly Ala Gin Val 1010 1015 1020 Asn Thr Leu Leu Leu Gly Glu Asn Asn Trp Pro Lys Ser Phe Thr Ser 1025 1030 1035 1040 Leu Trp Gin Leu Leu Thr Trp Leu Arg Val Gly Gin Arg Leu Asn Val 1045 1050 1055 Gly Ser Thr Thr Leu Gly Asn Leu Leu Ser Met Met Gin Ala Asp Pro 1060 1065 1070 Ala Ala Glu Ser Ser Ala Leu Leu Ala Ser Val Ala Gin Asn Leu Ser 1075 1080 1085 Ala Ala lie Ser Asn Arg Gin *** -71 -C Please read the notes on the back before filling this page), 11 丨 This paper size is applicable to the Chinese National Standard (CNS) M specification (210X297 mm) 509722 A8 B8 C8 D8 Patent application scope 1090 1095 (Please read the back Note: Please fill in this page again) Sequence ID No. 35: Pro Leu Ser Thr Ser Glu Leu Thr Ser Lys Leu Asn Ser lie Asp Thr 15 10 15 Phe Cys Glu Lys Thr Arg Leu Ser Phe Asn Gin Leu Met Asp Leu Thr 20 25 30 Ala Gin Gin Ser Tyr Ser Gin Ser Ser lie Asp Ala Lys Ala Ala Ser 35 40 45 Arg Tyr Val Arg Phe Gly Glu Thr Thr Pro Thr Arg Val Asn Val Tyr 50 55 60 Gly Ala Ala Tyr Leu Asn Ser Thr Leu Ala Asp Ala Ala Asp Gly Gin 65 70 75 80 Tyr Leu Trp lie Gin Thr Asp Gly Lys Ser Leu Asn Phe Thr Asp Asp 85 90 95 Thr Val Val Ala Leu Ala Gly Arg Ala Glu Lys Leu Val Arg Leu Ser 100 105 110 Ser Gin Thr Gly Leu Ser Phe Glu Glu Leu Asp Trp Leu lie Ala Asn 115 120 125 This paper size applies to Chinese National Standard (CNS) M specification (210 X 297 mm) -72- 509722 A8 B8 C8 D8 VI. Scope of patent application (please read the notes on the back before filling this page) Ala Ser Arg Ser Val Pro Asp His His Asp Lys lie Val Leu Asp Lys 130 135 140 Pro Val Leu Glu Ala Leu Ala Glu Tyr Val Ser Leu Lys Gin Arg Tyr 145 150 155 160 Gly Leu Asp Ala Asn Thr Phe Ala Thr Phe lie Ser Ala Val Asn Pro 165 170 175 Order Tyr Thr Pro Asp Gin Thr Pro Ser Phe Tyr Glu Thr Ala Phe Arg Ser 180 185 190 Ala AspGly Asn His Val lie Ala Leu Gly Thr Glu Val Lys Tyr Ala 195 200 205 Glu Asn Glu Gin Asp Glu Leu Ala Ala lie Cys Cys Lys Ala Leu Gly 210 215 220 Val Thr Ser Asp Glu Leu Leu Arg lie Gly Arg Tyr Cys Phe Gly Asn 225 230 235 240 Ala Gly Arg Phe Thr Leu Asp Glu Tyr Thr Ala Ser Gin Leu Tyr Arg 245 250 255 -73- This paper size applies to the Chinese National Standard (CNS) A4 specification (210 X 297 mm) 509722 A8 B8 C8 D8 6. Application scope Phe Gly Ala lie Pro Arg Leu Phe Gly Leu Thr Phe Ala Gin Ala Glu 260 265 270 lie Leu Trp Arg Leu Met Glu Gly Gly Lys Asp lie Leu Leu Gin Gin 275 280 285 _ (Please read first Note on the back, please fill out this page) Xxx Gly Gin Ala Lys Ser Leu Gin Pro Leu Ala lie Leu Arg Arg Thr 290 295 300 Glu Gin Val Leu Asp Trp Met Ser Pro Val Asn Leu Ser Leu Thr Tyr 305 310 315 320 Leu Gin Gly Met Val Ser Thr Gin Trp Ser Gly Thr Ala Thr Ala Glu 325 330 335 Met Phe Asn Phe Leu Glu Asn Val Cys Asp Ser Val Asn Ser Gin Ala 340 345 350 Xxx Thr Lys Glu Thr Met Asp Ser Ala Leu Gin Gin Lys Val Leu Arg 355 360 365 Ala Leu Ser Ala Gly Phe Gly lie Lys Ser Asn Val Met Gly lie Val 370 375 380 The paper Λ degree applies to the Chinese National Standard (CNS) A4 specification (210X297 mm) -74- 509722 A8 B8 C8 D8 Application scope Thr Phe Trp Leu Glu Lys lie Thr lie Gly Arg Asp Asn Pro Phe Thr 385 390 395 400 Leu Ala Asn Tyr Trp His Asp lie Gin Thr Leu Phe Ser His Asp Asn 405 410 415 Ala Thr Leu Glu Ser Leu Gin Thr Asp Thr Ser Leu Val lie Ala Thr 420 425 430 Gin Gin Leu Ser Gin Leu Val Leu lie Val Lys Trp Val Ser Leu Thr 435 440 445 Glu Gin Asp Leu Gin Leu Leu Thr Thr Tyr Pro Glu Arg Leu lie Asn 450 455 460 Gly lie Thr Asn Val Pro Val Pro Asn Pro Glu Leu Leu Leu Thr Leu 465 470 475 480 Ser Arg Phe Lys Gin Trp Glu Thr Gin Val Thr Val Ser Arg Asp Glu 485 490 495 Ala Met Arg Cys Phe Asp Gin Leu Asn Ala Asn Asp Met Thr Thr Glu 500 505 510 (Please read the notes on the back before filling out this page) Order · # · -75- This paper size applies to China National Standard (CNS) A4 specifications (210X297 (%) 509722 A8 B8 C8 D8 6. Scope of patent application (please read the notes on the back before filling this page) Asn Ala Gly Ser Leu lie Ala Thr Leu Tyr Glu Met Asp Lys Gly Thr 515 520 525 Gly Ala Gin Val Asn Thr Leu Leu Leu Gly Glu Asn Asn Trp Pro Lys 530 535 ♦ 540 Ser Phe Thr Ser Leu Trp Gin Leu Leu Thr Trp Leu Arg Val Gly Gin 545 550 555 560 Arg Leu Asn Val Gly Ser Thr Thr Leu Gly Asn Leu Leu Ser Met Met 565 570 575 Gin Ala Asp Pro Ala Ala Glu Ser Ser Ala Leu Leu Ala Ser Val Ala 580 585 590: Line 丨 Gin Asn Leu Ser Ala Ala lie Ser Asn Arg Gin * 595 600 Sequence ID No. 47: Met Asn Glu Ser Val Lys Glu lie Pro Asp Val Leu Lys Ser Gin Cys 1 5 10 15 Gly Phe Asn Cys Leu Thr Asp lie Ser His Ser Ser Phe Asn Glu Phe 20 25 30 This paper size applies to Chinese National Standard (Norway) A4 (210 X 297 mm) -76- 509722 A8 B8 C8 D8 6. Scope of patent application Arg Gin Gin Val Ser Glu His Leu Ser Trp Ser Glu Thr His Asp Leu 35 40 45 Tyr His Asp Ala Gin Gin Ala Gin Lys A sp Asn Arg Leu Tyr Glu Ala 50 55 60 Arg lie Leu Lys Arg Ala Asn Pro Gin Leu Gin Asn Ala Val His Leu 65 70 75 80 Ala lie Leu Ala Pro Asn Ala Glu Leu lie Gly Tyr Asn Gin Phe 85 90 95 Ser Gly Arg Ala Ser Gin Tyr Val Ala Pro Gly Thr Val Ser Ser Met 100 105 110 Phe Ser Pro Ala Ala Tyr Leu Thr Glu Leu Tyr Arg Glu Ala Arg Asn 115 120 125 Leu His Ala Ser Asp Ser Val Tyr Tyr Leu Asp Thr Arg Arg Pro Asp 130 135 140 Leu Lys Ser Met Ala Leu Ser Gin Gla Asn Met Asp lie Glu Leu Ser 145 150 155 160 ___; _ This paper size applies to China National Standard (CNS) A4 (210 X 297 mm) (Please (Please read the precautions on the back before filling this page) 、 509722 A8 B8 C8 D8 Patent application scope (please read the precautions on the back before filling this page) Thr Leu Ser Leu Ser Asn Glu Leu Leu Leu Glu Ser lie Lys Thr Glu 165 170 175 Ser Lys Leu Glu Asn Tyr Thr Lys Val Met Glu Met Leu Ser Thr Phe 180 185 190 Arg Pro Ser Gly Ala Thr Pro Tyr His Asp Ala Tyr Glu Asn Val Arg 195 200 205 Glu Val lie Gin Leu Gin A sp Pro Gly Leu Glu Gin Leu Asn Ala Ser 210 215 220 Pro Ala lie Ala Gly Leu Met His Gin Ala Ser Leu Leu Gly lie Asn 225 230 235 240 Ala Ser lie Ser Pro Glu Leu Phe Asn lie Leu Thr Glu Glu lie Thr 245 250 255 Glu Gly Asn Ala Glu Glu Leu Tyr Lys Lys Asn Phe Gly Asn lie Glu 260 265 270 Pro Ala Ser Leu Ala Met Pro Glu Tyr Leu Lys Arg Tyr Tyr Asn Leu 275 280 285 This paper is in accordance with the Chinese National Standard (CNS) A4 specifications (210 X 297 mm) -78- 509722 A8 B8 C8 D8 Patent application scope (please read the notes on the back before filling this page) Ser Asp Glu Glu Leu Ser Gin Phe lie Gly Lys Ala Ser Asn Phe Gly 290 295 300 Gin Gin Glu Tyr Ser Asn Asn Gin Leu lie Thr Pro Val Val Asn Ser 305 310 315 320 Ser Asp Gly Thr Val Lys Val Tyr Arg lie Thr Arg Glu Tyr Thr Thr 325 330 335 Order — Asn Ala Tyr Gin Met Asp Val Glu Leu Phe Pro Phe Gly Gly Glu Asn 340 345 350 Tyr Arg Leu Asp Tyr Lys Phe Lys Asn Phe Tyr Asn Ala Ser Tyr Leu 355 360 365 Ser lie Lys Leu Asn Asp Lys Arg Glu Leu Val Arg Thr Glu Gl y Ala 370 375 380 Pro Gin Val Asn lie Glu Tyr Ser Ala Asn lie Thr Leu Asn Thr Ala 385 390 395 400 Asp lie Ser Gin Pro Phe Glu lie Gly Leu Thr Arg Val Leu Pro Ser 405 410 415 -79- Paper Size Applicable to China National Standard (CNS) A4 specification (210 X 297 mm) 509722 A8 B8 C8 D8 Patent application scope (please read the notes on the back before filling this page) Gly Ser Trp Ala Tyr Ala Ala Ala Lys Phe Thr Val Glu Glu Tyr Asn 420 425 430 Gin Tyr Ser Phe Leu Leu Lys Leu Asn Lys Ala lie Arg Leu Ser Arg 435 4-40 445 Ala Thr Glu Leu Ser Pro Thr lie Leu Glu Gly lie Val Arg Ser Val 450 455 460 Asn Leu Gin Leu Asp lie Asn Thr Asp Val Leu Gly Lys Val Phe Leu. Order i 465 470 475 480 Thr Lys Tyr Tyr Met Gin Arg Tyr Ala lie His Ala Glu Thr Ala Leu 485 490 495: Line 丨 lie Leu Cys Asn Ala Pro lie Ser Gin Arg Ser Tyr Asp Asn Gin Pro 500 505 510 Ser Gin Phe Asp Arg Leu Phe Asn Thr Pro Leu Leu Asn Gly Gin Tyr 515 520 525 Phe Ser Thr Gly Asp Glu Glu lie Asp Leu Asn Ser Gly Ser Thr Gly 530 535 540 -80 - This paper is compliant with Chinese National Standard (CNS) A4 specifications (210X297 mm) 509722 A8 B8 C8 D8 Patent application scope Asp Trp Arg Lys Thr lie Leu Lys Arg Ala Phe Asn lie Asp Asp Val 545 550 555 560 Ser Leu Phe Arg Leu Leu Lys lie Thr Asp Hi s Asp Asn Lys Asp Gly 565 570 575 Lys lie Lys Asn Asn Leu Lys Asn Leu Ser Asn Leu Tyr lie Gly Lys 580 585 590 Leu Leu Ala Asp lie His Gin Leu Thr lie Asp Glu Leu Asp Leu Leu 595 600 605 Leu lie Ala Val Gly Glu Gly Lys Thr Asn Leu Ser Ala lie Ser Asp 610 615 620 Lys Gin Leu Ala Thr Leu lie Arg Lys Leu Asn Thr lie Thr Ser Trp 625 630 635 640 Leu His Thr Gin Lys Trp Ser Val Phe Gin Leu Phe lie Met Thr Ser 645 650 655 Thr Ser Tyr Asn Lys Thr Leu Thr Pro Glu lie Lys Asn Leu Leu Asp 660 665 670 (Please read the notes on the back before filling this page)-" I # This paper is compliant with China National Standard (CNS) A4 specifications (210X297 mm) -81- 509722 A8 B8 C8 D8 VI. Patent application scope Thr Val Tyr His Gly Leu Gin Gly Phe Asp Lys Asp Lys Ala Asp Leu 675 680 68 5 Leu His Val Met Ala Pro Tyr lie Ala Ala Thr Leu Gin Leu Ser Ser 690 695 700 Glu Asn Val Ala His Ser Val Leu Leu Trp Ala Asp Lys Leu Gin Pro 705 710 715 720 Gly Asp Gly Ala Met Thr Ala Glu Lys Phe Trp Asp Trp Leu Asn Thr 725 730 735 Lys Tyr Thr Pro Gly Ser Ser Glu Ala Val Glu Thr Gin Glu His lie 740 745 750 Val Gin Tyr Cys Gin Ala Leu Ala Gin Leu Glu Met Val Tyr His Ser 755 760 765 Thr Gly lie Asn Glu Asn Ala Phe Arg Leu Phe Val Thr Lys Pro Glu 770 775 780 Met Phe Gly Ala Ala Thr Gly Ala Ala Pro Ala His Asp Ala Leu Ser 785 790 795 800 -82- ........... ........... attack ........, 玎 :: ............ line (Please read the precautions on the back before filling in this page) The paper size applies to the Chinese National Standard (CNS) A4 (210X297 mm) 509722 A8 B8 C8 D8 Patent Application Range Leu lie Met Leu Thr Arg Phe Ala Asp Trp Val Asn Ala Leu Gly Glu 805 810 815 Lys Ala Ser Ser Val Leu Ala Ala Phe Glu Ala Asn Ser Leu Thr Ala 820 825 830 Glu Gin Leu Ala Asp Ala Met Asn Leu Asp Ala Asn Le u Leu Leu Gin 835 840 845 Ala Ser lie Gin Ala Gin Asn His Gin His Leu Pro Pro Val Thr Pro 850 855 860 Glu Asn Ala Phe Ser Cys Trp Thr Ser lie Asn Thr lie Leu Gin Trp 865 870 875 880 Val Asn Val Ala Gin Gin Leu Asn Val Ala Pro Gin Gly Val Ser Ala 885 890 895 Leu Val Gly Leu Asp Tyr lie Gin Ser Met Lys Glu Thr Pro Thr Tyr 900 905 910 Ala Gin Trp Glu Asn Ala Ala Gly Val Leu Thr Ala Gly Leu Asn Ser 915 920 925 -83- This paper size applies to Chinese National Standard (CNS) A4 (210X297 mm) (Please read the precautions on the back before filling this page) 509722 A8 B8 C8 D8 patent application scope (please read the notes on the back before filling this page) Gin Gin Ala Asn Thr Leu His Ala Phe Leu Asp Glu Ser Arg Ser Ala 930 935 940 Ala Leu Ser Thr Tyr Tyr lie Arg Gin Val Ala Lys Ala Ala Ala Ala 945 950 955 960 lie Lys Ser Arg Asp Asp Leu Tyr Gin Tyr Leu Leu lie Asp Asn Gin 965 970 975 Val Ser Ala Ala lie Lys Thr Thr Arg lie Ala Glu Ala lie Ala Ser 980 985 990 lie Gin Leu Tyr Val Asn Arg Ala Leu Glu Asn Val Glu Glu Asn Ala 995 1000 1005 Asn Ser Gly Val lie Ser Arg Gin Phe Phe lie Asp Trp Asp Lys Tyr 1010 1015 1020 Asn Lys Arg Tyr Ser Thr Trp Ala Gly Val Ser Gin Leu Val Tyr Tyr 1025 1030 1035 1040 Pro Glu Asn Tyr lie Asp Pro Thr Met Arg lie Gly Gin Thr Lys Met 1045 1050 1055 -84- This paper size applies to China National Standard (CNS) A4 size (210X297 mm) 509722 A8 B8 C8 D8 Scope of patent application Met Asp Ala Leu Leu Gin Ser Val Ser Gin Ser Gin Leu Asn Ala Asp 1060 1065 1070 Thr Val Glu Asp Ala Phe Met Ser Tyr Leu Thr Ser Phe Glu Gin Val 1075 1080 1085 Ala Asn Leu Lys Val lie Ser Ala Tyr His Asp Asn lie Asn Asn Asp 1090 1095 1100 Gin Gly Leu Thr Tyr Phe lie Gly Leu Ser Glu Thr Asp Ala Gly Glu 1105 1110 1115 1120 Tyr Tyr Trp Arg Ser Val Asp His Ser Lys Phe Asn Asp Gly Lys Phe 1125 1130 1135 Ala Ala Asn Ala Trp Ser Glu Trp His Lys lie Asp Cys Pro lie Asn 1140 1145 1150 Pro Tyr Lys Ser Thr lie Arg Pro Val lie Tyr Lys Ser Arg Leu Tyr 1155 1160 1165 Leu Leu Trp Leu Glu Gin Lys Glu lie Thr Lys Gin Thr Gly Asn Ser 1170 1175 1180 (Please read the precautions on the back before filling this page) Order — f -85- This paper size applies to Chinese National Standards (CNS) A4 specification (210 X 297 mm) 509722 A8 B8 C8 D8 Patent application scope (please read the notes on the back before filling this page) Lys Asp Gly Tyr Gin Thr Glu Thr Asp Tyr Arg Tyr Glu Leu Lys Leu 1185 1190 1195 1200 Ala His lie Arg Tyr Asp Gly Thr Trp Asn Thr Pro lie Thr Phe Asp 1205 1210 1215 Val Asn Lys Lys lie Ser Glu Leu Lys Leu Glu Lys Asn Arg Ala Pro 1220 1225 1230 G ly Leu Tyr Cys Ala Gly Tyr Gin Gly Glu Asp Thr Leu Leu Val Met 1235 1240 1245 Phe Tyr Asn Gin Gin Asp Thr Leu Asp Ser Tyr Lys Asn Ala Ser Met 1250 1255 1260 Gin Gly Leu Tyr lie Phe Ala Asp Met Ala Ser Lys Asp Met Thr Pro 1265 1270 1275 1280 Glu Gin Ser Asn Val Tyr Arg Asp Asn Ser Tyr Gin Gin Pin Asp Thr 1285 1290 1295 Asn Asn Val Arg Arg Val Asn Asn Arg Tyr Ala Glu Asp Tyr Glu lie 1300 1305 1310 -86-ben Paper size applies Chinese National Standard (CNS) A4 specification (210 X 297 mm) 509722 A8 B8 C8 D8 VI. Patent application scope (please read the notes on the back before filling this page) Pro Ser Ser Val Ser Ser Arg Lys Asp Tyr Gly Trp Gly Asp Tyr Tyr 1315 1320 1325 Leu Ser Met Val Tyr Asn Gly Asp lie Pro Thr lie Asn Tyr Lys Ala 1330 1335: 1340 Ala Ser Ser Asp Leu Lys lie Tyr lie Ser Pro Lys Leu Arg lie lie 1345 1350 1355 1360 Hi s Asn Gly Tyr Glu Gly Gin Lys Arg Asn Gin Cys Asn Leu Met Asn 1365 1370 1375 Lys Tyr Gly Lys Leu Gly Asp Lys Phe lie Val Tyr Thr Ser Leu Gly 1380 1385 1390 V al Asn Pro Asn Asn Ser Ser Asn Lys Leu Met Phe Tyr Pro Val Tyr 1395 1400 1405 Gin Tyr Ser Gly Asn Thr Ser Gly Leu Asn Gin Gly Arg Lea Leu Phe 1410 1415 1420 His Arg Asp Thr Thr Tyr Pro Ser Lys Val Glu Ala Trp lie Pro Gly 1425 1430 1435 1440 The paper Λ degree applies to Chinese National Standard (CNS) A4 (210 X 297 mm) -87- 509722 A8 B8 C8 D8 -JL. Scope of patent application (please read the precautions on the back first) (Refill this page) Ala Lys Arg Ser Leu Thr Asn Gin Asn Ala Ala lie Gly Asp Asp Tyr 1445 1450 1455 Ala Thr Asp Ser Leu Asn Lys Pro Asp Asp Leu Lys Gin Tyr lie Phe 1460 1465 1470 Met Thr Asp Ser Lys Gly Thr Ala Thr Asp Val Ser Gly Pro Val Glu 1475 1480 1485 lie Asn Thr Ala lie Ser Pro Ala Lys Val Gin lie lie Val Lys Ala 1490 1495 1500 Gly Gly Lys Glu Gin Thr Phe Thr Ala Asp Lys Asp Val Ser lie Gin 1505 1510 1515 1520 Pro Ser Pro Ser Phe Asp Glu Met Asn Tyr Gin Phe Asn Ala Leu Glu 1525 1530 1535 lie Asp GLy Ser Gly Leu Asn Phe lie Asn Asn Ser Ala Ser lie Asp 1540 1545 1550 Val Th r Phe Thr Ala Phe Ala Glu Asp Gly Arg Lys Leu Gly Tyr Glu 1555 1560 1565 -88- This paper size applies to China National Standard (CNS) A4 (210 X 297 mm) 509722 A8 B8 C8 D8 Ser Phe Ser lie Pro Val Thr Leu Lys Val Ser Thr Asp Asn Ala Leu 1570 1575 1580 Thr Leu His His Asn Glu Asn Gly Ala Gin Tyr Met Gin Trp Gin Ser 1585 1590 1595 1600 Tyr Arg Thr Arg Leu Asn Thr Leu Phe Ala Arg Gin Leu Val Ala Arg 1605 1610 1615 Ala Thr Thr Gly lie Asp Thr lie Leu Ser Met Glu Thr Gin Asn lie 1620 1625 1630 Gin Glu Pro Gin Leu Gly Lys Gly Phe Tyr Ala Thr Phe Val lie Pro 1635 1640 1645 Pro Tyr Asn Leu Ser Thr His Gly Asp Glu Arg Trp Phe Lys Leu Tyr 1650 1655 1660 lie Lys His Val Val Asp Asn Asn Ser His lie lie Tyr Ser Gly Gin 1665 1670 1675 1680 Leu Thr Asp Thr Asn lie Asn lie Thr Leu Phe lie Pro Leu Asp Asp 1685 1690 1695 This paper size applies to Chinese National Standard (CNS) A4 (210 X 297 mm) (Please read the precautions on the back before filling this page) -89- 509722 A8 B8 C8 D8 6. Scope of patent application (Please read the notes on the back before filling this page) Val Pro Leu Asn Gin Asp Tyr His Ala Lys Val Tyr Met Thr Phe Lys 1700 1705 1710 Lys Ser Pro Ser Asp Gly Thr Trp Trp Gly Pro His Phe Val Arg Asp 1715 1.720 1725 Asp Lys Gly lie Val Thr lie Asn Pro Lys Ser lie Leu Thr His Phe 1730 1735 1740 Glu Ser Val Asn Val Leu Asn Asn lie Ser Ser Glu Pro Met Asp Phe. Order. 1745 1750 1755 1760 Ser Gly Ala Asn Ser Leu Tyr Phe Trp Glu Leu Phe Tyr Tyr Thr Pro 1765 1770 Π75: Line — Met Leu Val Ala Gin Arg Leu Leu His Glu Gin Asn Phe Asp Glu Ala 1780 1785 1790 Asn Arg Trp Leu Lys Tyr Val Trp Ser Pro Ser Gly Tyr He Val His 1795 1800 1805 Gly Gin lie Gin Asn Tyr Gin Trp Asn Val Arg Pro Leu Leu Glu Asp 1810 1815 1820 -90- This paper size applies to China National Standard (CNS) A4 specifications (210 X 297 mm) 509722 A8 B8 C8 D8 Patent Application Range Thr Ser Trp Asn Ser Asp Pro Leu Asp Ser Va1 Asp Pro Asp Ala Val 1825 1830 1835 1840 Ala Gin His Asp Pro M et His Tyr Lys Val Ser Thr Phe Met Arg Thr 1845 1850 1855 Leu Asp Leu Leu lie Ala Arg Gly Asp His Ala Tyr Arg Gin Leu Glu 1860 1865 1870 Arg Asp Thr Leu Asn Glu Ala Lys Met Trp Tyr Met Gin Ala Leu His 1875 1880 1885 Leu Leu Gly Asp Bys ys Pro Tyr Leu Pro Leu Ser Thr Thr Trp Ser Asp 1890 1895 1900 Pro Arg Leu Asp Arg Ala Ala Asp lie Thr Thr Gin Asn Ala His Asp 1905 1910 1915 1920 Ser Ala lie Val Ala Leu Arg Gin Asn lie Pro Thr Pro Ala Pro Leu 1925 1930 1935 Ser Leu Arg Ser Ala Asn Thr Leu Thr Asp Leu Phe Leu Pro Gin lie 1940 1945 1950 This paper size applies to China National Standard (CNS) A4 (210 X 297 mm> ( (Please read the precautions on the back before filling this page) • 1T · -91- 509722 A8 B8 C8 D8 VI. Application scope Asn Glu Val Met Met Asn Tyr Trp Gin Thr Leu Ala Gin Arg Val Tyr 1955 1960 1965 Asn Leu Arg His Asn Leu Ser lie Asp Gly Gin Pro Leu Tyr Leu Pro 1970 1975 · 1980 lie Tyr Ala Thr Pro Ala Asp Pro Lys Ala Leu Leu Ser Ala Ala Val 1985 1990 1995 2000 Ala Thr Ser Gin Gly Gly Gly Gly Lys Leu Pro Glu Ser Phe Met Ser Leu 2005 2010 2015 Trp Arg Phe Pro His Met Leu Glu Asn Ala Arg Gly Met Val Ser Gin 2020 2025 2030 Leu Thr Gin Phe Gly Ser Thr Leu Gin Asn lie Glu Arg Gin Asp 2035 2040 2045 Ala Glu Ala Leu Asn Ala Leu Leu Gin Asn Gin Ala Ala Glu Leu lie 2050 2055 2060 Leu Thr Asn Leu Ser lie Gin Asp Lys Thr lie Glu Glu Leu Asp Ala 2065 2070 2075 2080 Standard (CNS) A4 specification (210X297 mm) (Please read the precautions on the back before filling out this page) ·,: I: line 丨 -92- 509722 A8 B8 C8 D8 T \ Patent application scope Glu Lys Thr Val Leu Glu Lys Ser Lys Ala Gly Ala Gin Ser Arg Phe 2085 2090 2095 Asp Ser Tyr Gly Lys Leu Tyr Asp Glu Asn lie Asn Ala Gly Glu Asn 2100 2105 2110 Gin Ala Met Thr Leu Arg Ala Ser Ala Ala Gly Leu Thr Thr Ala Val 2115 2120 2125 Gin Ala Ser Arg Leu Ala Gly Ala Ala Ala Asp Leu Val Pro Asn lie 2130 2135 2140 Phe Gly Phe Ala Gly Gly Gly Gly Ser Arg Trp Gly Ala lie Ala Glu Ala 2145 2150 2155 2160 Th r Gly Tyr Val Met Glu Phe Ser Ala Asn Val Met Asn Thr Glu Ala 2165 2170 2175 Asp Lys lie Ser Gin Ser Glu Thr Tyr Arg Arg Arg Arg gin Gin Glu Trp 2180 2185 2190 Glu lie Gin Arg Asn Asn Ala Glu Ala Glu Leu Lys Gin lie Asp Ala 2195 2200 2205 (Please read the notes on the back before filling this page) Order — ♦ This paper size applies to China National Standard (CNS) A4 (210 X 297 mm) -93- 509722 A8 B8 C8 D8 Scope of patent application (please read the notes on the back before filling this page) Gin Leu Lys Ser Leu Ala Val Arg Arg Glu Ala Ala Val Leu Gin Lys 2210 2215 2220 Thr Ser Leu Lys Thr Gin Gin Glu Gin Thr Gin Ser Gin Leu Ala Phe 2225 2230 2235 2240 Leu Gin Arg Lys Phe Ser Asn Gin Ala Leu Tyr Asn Trp Leu Arg Gly 2245 2250 2255 Arg Leu Ala Ala lie Tyr Phe Gin Phe Tyr Asp Leu Ala Val Ala Arg 2260 2265 2270 Cys Leu Met Ala Glu Gin Tyr Arg Trp Glu Leu Asn Asp Asp Ser 2275 2280 2285 Ala Arg Phe lie Lys Pro Gly Ala Trp Gin Gly Thr Tyr Ala Gly Leu 2290 2295 2300 Leu Ala Gly Glu Thr Leu Met Leu Ser Leu Ala Gin Met Glu Asp Ala 2305 2310 2315 2320 His Leu Lys Arg Asp Lys Arg Ala Leu Glu Val Glu Arg Thr Val Ser 2325 2330 2335 This paper size applies to Chinese National Standard (CNS) A4 (210X297 mm) -94- 509722 A8 B8 C8 D8 VI. Patent Application Range Leu Ala Glu Val Tyr Ala Gly Leu Pro Lys Asp Asn Gly Pro Phe Ser 2340 2345 2350 Leu Ala Gin Glu lie Asp Lys Leu Val Ser Gin Gly Ser Gly Ser Ala 2355 2360 2365 Gly Ser Gly Asn Asn Asn Leu Ala Phe Gly Ala Gly Thr Asp Thr Lys 2370 2375 2380 Thr Ser Leu Gin Ala Ser Val Ser Phe Ala Asp Leu Lys lie Arg Glu 2385 2390 2395 2400 Asp Tyr Pro Ala Ser Leu Gly Lys lie Arg Arg lie lys Gin lie Ser 2405 2410 2415 Val Thr Leu Pro Ala Leu Leu Gly Pro Tyr Gin Asp Val Gin Ala lie 2420 2425 2430 Leu Ser Tyr Gly Asp Lys Ala Gly Leu Ala Asn Gly Cys Glu Ala Leu 2435 2440 2445 Ala Val Ser His Gly Met Asn Asp Ser Gly Gin Phe Gin Leu Asp Phe 2450 2455 2460 (Please read the precautions on the back before filling out this page) •, ^ J · MW, this paper size applies to China Standard (CNS) A4 specification (210 X 297 mm) -95- 509722 A8 B8 C8 D8 Patent application scope (please read the notes on the back before filling this page) Asn Asp Gly Lys Phe Leu Pro Phe Glu Gly lie Ala lie Asp Gin Gly 2465 2470 2475 2480 Thr Leu Thr Leu Ser Phe Pro Asn Ala Ser Met Pro Glu Lys Gly Lys 2485 2490 2495 Gin Ala Thr Met Leu Lys Thr Leu Asn Asp lie lie Leu Hi s lie Arg 2500 2505 2510 Tyr Thr lie Lys 2516 Sequence Identification Number 49: Leu lie Gly Tyr Asn Asn Gin Phe Ser Gly Arg Ala Ser Gin Tyr Val 1 5 10 15 Ala Pro Gly Thr Val Ser Ser Met Phe Ser Pro Ala Ala Tyr Leu Thr 20 25 30 Glu Leu Tyr Arg Glu Ala Arg Asn Leu His Ala Ser Asp Ser Val Tyr 35 40 45 Tyr Leu Asp Thr Arg Arg Pro Asp Leu Lys Ser Met Ala Leu Ser Gin 50 55 60 This paper size applies to China National Standard (CNS) A4 (210X297) (%) -96- 509722 A8 B8 C8 D8 VI. Application scope Gin Asn Met Asp lie Glu Leu Ser Thr Leu Ser Leu Ser Asn G1u Leu 65 70 75 80 Leu Leu Glu Ser lie Lys Thr Glu Ser Lys Leu Glu Asn Ty r Thr Lys 85 90 95 Val Met Glu Met Leu Ser Thr Phe Arg Pro Ser Gly Ala Thr Pro Tyr 100 105 110 His Asp Ala Tyr Glu Asn Val Arg Glu Val lie Gin Leu Gin Asp Pro 115 120 125 Gly Leu Glu Gin Leu Asn Ala Ser Pro Ala lie Ala Gly Leu Met His 130 135 140 Gin Ala Ser Leu Leu Gly lie Asn Ala Ser lie Ser Pro Glu Leu Phe 145 150 155 160 Asn lie Leu Thr Glu Glu lie Thr Glu Gly Asn Ala Glu Glu Leu Tyr 165 170 175 Lys Lys Asn Phe Gly Asn lie Glu Pro Ala Ser Leu Ala Met Pro Glu 180 185 190 This paper size applies to China National Standard (CNS) A4 size (210X297 mm) (Please read the precautions on the back before filling the book page) -97 · 509722 A8 B8 C8 D8 Patent Application Fan Park Tyr Leu Lys Arg Tyr Tyr Asn Leu Ser Asp Glu Glu Leu Ser Gin Phe 195 200 205 lie Gly Lys Ala Ser Asn Phe Gly Gin Gin Glu Tyr Ser Asn Asn Gin 210 215. 220 Leu lie Thr Pro Val Val Asn Ser Ser Asp Gly Thr Val Lys Val Tyr 225 230 235 240 Arg lie Thr Arg Glu Tyr Thr Thr Asn Ala Tyr Gin Met Asp Val Glu 245 250 255 Leu Phe Pro Phe Gly Gly Glu Asn Tyr Arg Leu Asp Tyr Lys Phe Lys 260 265 270 Asn Phe Tyr Asn Ala Ser Tyr Leu Ser lie Lys Leu Asn Asp Lys Arg 275 280 285 Glu Leu Val Arg Thr Glu Gly Ala Pro Gin Val Asn lie Glu Tyr Ser 290 295 300 Ala Asn lie Thr Leu Asn Thr Ala Asp lie Ser Gin Pro Phe Glu lie 305 310 315 320 ............-... ......... tr ........ line · (Please read the precautions on the back before filling in this page) -98 · This paper size Applicable to Chinese National Standard (CNS) M specification (210X297 mm) 509722 A8 B8 C8 D8 VI. Patent application scope Gly Leu Thr Arg Val Leu Pro Ser Gly Ser Trp Ala Tyr Ala Ala Ala Ala 325 3 30 335 Lys Phe Thr Val Glu Glu Tyr Asn Gin Tyr Ser Phe Leu Leu Lys Leu 340 345 350 Asn Lys Ala lie Arg Leu Ser Arg Ala Thr Glu Leu Ser Pro Thr lie 355 360 365 Leu Glu Gly lie Val Arg Ser Val Asn Leu Gin Leu Asp lie Asn Thr 370 375 380 Asp Val Leu Gly Lys Val Phe Leu Thr Lys Tyr Tyr Met Gin Arg Tyr 385 390 395 400 Ala lie His Ala Glu Thr Ala Leu lie Leu Cys Asn Ala Pro lie Ser 405 410 415 Gin Arg Ser Tyr Asp Asn Gin Pro Ser Gin Phe Asp Arg Leu Phe Asn 420 425 430 Thr Pro Leu Leu Asn Gly Gin Tyr Phe Ser Thr Gly Asp Glu Glu lie 435 440 445 This paper size applies to the Chinese National Standard (CNS) A4 specification (210X297 Mm) (Please read the notes on the back before filling out this page) -99- 509722 A8 B8 C8 D8 Patent Application Range Asp Leu Asn Ser Gly Ser Thr Gly Asp Trp Arg Lys Thr He Leu Lys 450 455 460 Arg Ala Phe Asn lie Asp Asp Val Ser Leu Phe Arg Leu Leu Lys lie 465 470 475 480 Thr Asp Hi s Asp Asn Lys Asp Gly Lys lie Lys Asn Asn Leu Lys Asn 485 490 495 Leu Ser Asn Leu Tyr lie Gly Lys Leu Leu Ala Asp lie Hi s Gin Leu 500 505 510 Thr lie Asp Glu Leu Asp Leu Leu Leu lie Ala Val Gly Glu Gly Lys 515 520 525 Thr Asn Leu Ser Ala lie Ser Asp Lys Gin Leu Ala Thr Leu lie Arg 530 535 540 Lys Leu Asn Thr lie Thr Ser Trp Leu His Thr Gin Lys Trp Ser Val 545 550 555 560 Phe Gin Leu Phe lie Met Thr Ser Thr Ser Tyr Asn Lys Thr Leu Thr 565 570 575 This paper size applies to China National Standard (CNS) A4 (210X297 mm) ---------------- ------- 、 Installing ............ TΓ ........ line. (Please (Please read the notes on the back before filling this page) -100- 509722 A8 B8 C8 D8 Patent Application Pro Glu lie Lys Asn Leu Leu Asp Thr Val Tyr His Gly Leu Gin Gly 580 585 59 0 Phe Asp Lys Asp Lys Ala Asp Leu Leu His Val Met Ala Pro Tyr lie 595 6Ό0 605 Ala Ala Thr Leu Gin Leu Ser Ser Glu Asn Val Ala His Ser Val Leu 610 615 620 Leu Trp Ala Asp Lys Leu Gin Pro Gly Asp Gly Ala Met Thr Ala Glu 625 630 635 640 Lys Phe Trp Asp Trp Leu Asn Thr Lys Tyr Thr Pro Gly Ser Ser Glu 645 650 655 Ala Val Glu Thr Gin Glu His lie Val Gin Tyr Cys Gin Ala Leu Ala 660 665 670 Gin Leu Glu Met Val Tyr His Ser Thr Gly lie Asn Glu Asn Ala Phe 675 680 685 Arg Leu Phe Val Thr Lys Pro Glu Met Phe Gly Ala Ala Thr Gly Ala 690 695 700 (Please read the notes on the back before filling this page)-# .Order 丨 This paper size applies Chinese National Standard (CNS) M specification (210X297 mm) -101- 509722 A8 B8 C8 D8 Patent application scope Ala Pro Ala His Asp Ala Leu Ser Leu lie Met Leu Thr Arg Phe Ala 705 710 715 720 Asp Trp Val Asn Ala Leu Gly Glu Lys Ala Ser Ser Val Leu Ala Ala 725 730 735 Phe Glu Ala Asn Ser Leu Thr Ala Glu Gin Leu Ala Asp Ala Met Asn 740 745 750 Leu Asp Ala Asn Leu Leu Leu Gin Ala Ser lie Gin Ala Gin Asn His 755 760 765 Gin His Leu Pro Pro Val Thr Pro Glu Asn Ala Phe Ser Cys Trp Thr 770 775 780 Ser lie Asn Thr lie Leu Gin Trp Val Asn Val Ala Gin Gin Leu Asn 785 790 795 800 Val Ala Pro Gin Gly Val Ser Ala Leu Val Gly Leu Asp Tyr lie Gin 805 810 815 Ser Met Lys Glu Thr Pro Thr Tyr Ala Gin Trp Glu Asn Ala Ala Gly 820 825 830 This paper size applies to the Chinese National Standard (CNS) A4 specifications ( 210X297 mm) ............ Bon ............... Order ... ......... Line · (Please read the precautions on the back before filling this page) -102- 509722 A8 B8 C8 D8 VI. Patent Application Scope (Please read the precautions on the back before filling this page) Val Leu Thr Ala Gly Leu Asn Ser Gin Gin Ala Asn Thr Leu His Ala 835 840 845 Phe Leu Asp Glu Ser Arg Ser Ala Ala Leu Ser Thr Tyr Tyr lie Arg 850 855 860 Gin Val Ala Lys Ala Ala Ala lie Lys Ser Arg Asp Asp Leu Tyr 865 870 875 880 Gin Tyr Leu Leu lie Asp Asn Gin Val Ser Ala Ala lie Lys Thr Thr 885 890 895 Arg lie Ala Glu Ala lie Ala Ser lie Gin Leu Tyr Val Asn Arg Ala 900 905 910 Leu Glu Asn Val Glu Glu Asn Ala Asn Ser Gly Val lie Ser Arg Gin 915 920 925 Phe Phe lie Asp Trp Asp Lys Tyr Asn Lys Arg Tyr Ser Thr Trp Ala 930 935 940 Gly Val Ser Gin Leu Val Tyr Tyr Pro Glu Asn Tyr lie Asp Pro Thr 945 950 955 960 -103- This paper size applies to Chinese National Standard (CNS) A4 specification (210 X 2974%) 509722 A8 B8 C8 D8 VI. Patent application scope (please read the precautions on the back before filling this page) Met Arg lie Gly Gin Thr Lys Met Met Asp Ala Leu Leu Gin Ser Val 965 970 975 Ser G in Ser Gin Leu Asn Ala Asp Thr Val Glu Asp Ala Phe Met Ser 980 985 990 Tyr Leu Thr Ser Phe Glu Gin Val Ala Asn Leu Lys Val lie Ser Ala 995 1000 1005 Tyr His Asp Asn lie Asn Asn Asp Gin Gly Leu Thr Tyr Phe lie Gly 1010 1015 1020 Leu Ser Glu Thr Asp Ala Gly Glu Tyr Tyr Trp Arg Ser Val Asp His 1025 1030 1035 1040 Ser Lys Phe Asn Asp Gly Lys Phe Ala Ala Asn Ala Trp Ser Glu Trp 1045 1050 1055 His Lys lie Asp Cys Pro lie Asn Pro Tyr Lys Ser Thr lie Arg Pro 1060 1065 1070 Val lie Tyr Lys Ser Arg Leu Tyr Leu Leu. Trp Leu Glu Gin Lys Glu 1075 1080 1085 This paper size applies to China National Standard (CNS) A4 specifications (210 X 297 Mm) -104- 509722 A8 B8 C8 D8 VI. Patent Application Range lie Thr Lys Gin Thr Gly Asn Ser Lys Asp Gly Tyr Gin Thr Glu Thr 1090 1095 1100 Asp Tyr Arg Tyr Glu Leu Lys Leu Ala-His lie Arg Tyr Asp Gly Thr 1105 1110 1115 1120 Trp Asn Thr Pro lie Thr Phe Asp Val Asn Lys Lys lie Ser Glu Leu 1125 1130 1135 Lys Leu Glu Lys Asn Arg Ala Pro Gly Leu Tyr Cys Ala Gly Tyr Gin 1140 1145 1150 Gly Glu Asp Thr Leu Leu Val Met Phe Tyr Asn Gin Gin Asp Thr Leu 1155 1160 1165 Asp Ser Tyr Lys Asn Ala Ser Met Gin Gly Leu Tyr lie Phe Ala Asp 1170 1175 1180 Met Ala Ser Lys Asp Met Thr Pro Glu Gin Ser Asn Val Tyr Arg Asp 1185 1190 1195 1200 Asn Ser Tyr Gin Gin Phe Asp Thr Asn Asn. Val Arg Arg Val Asn Asn 1205 1210 1215 This paper size applies to China National Standard (CNS) A4 (210 X 297 mm) ) (Please read the notes on the back before filling this page) -105- 509722 A8 B8 C8 D8 Patent Application Range Arg Tyr Ala Glu Asp Tyr Glu lie Pro Ser Ser Val Ser Ser Arg Lys 1220 1225 1230. Asp Tyr Gly Trp Gly Asp Tyr Tyr Leu Ser Met Val Tyr Asn Gly Asp 1235 1240 1245 lie Pro Thr lie Asn Tyr Lys Ala Ala Ser Ser Asp Leu Lys lie Tyr 1250 1255 1260 lie Ser Pro Lys Leu Arg lie lie Hi s Asn Gly Tyr Glu Gly Gin Lys 1265 1270 1275 1280 Arg Asn Gin Cys Asn Leu Met Asn Lys Tyr Gly Lys Leu Gly Asp Lys 1285 1290 1295 Phe lie Val Tyr Thr Ser Leu Gly Val Asn Pro Asn Asn Ser Ser Asn 1300 1305 1310 Lys Leu Met Phe Tyr Pro Val Tyr Gin Tyr Ser Gly Asn Thr Ser Gly 1315 1320 1325 Leu Asn Gin Gly Arg Leu Leu Phe His Arg. Asp Thr Thr Tyr Pro Ser 1330 1335 1340 This paper size applies to China National Standard (CNS) A4 (210 X 297 mm) .............. ....... A ........, 玎 ........ line · (Please read the Please fill in this page again) -106- 509722 A8 B8 C8 D8 VI. Application scope of patents Lys Val Glu Ala Trp lie Pro Gly Ala Lys Arg Se r Leu Thr Asn Gin 1345 1350 1355 1360 Asn Ala Ala lie Gly Asp Asp Tyr Ala Thr Asp Ser Leu Asn Lys Pro 1365 1370 1375 Asp Asp Leu Lys Gin Tyr lie Phe Met Thr Asp Ser Lys Gly Thr Ala 1380 1385 1390 Thr Asp Val Ser Gly Pro Val Glu lie Asn Thr Ala lie Ser Pro Ala 1395 1400 1405 Lys Val Gin lie lie Val Lys Ala Gly Gly Lys Glu Gin Thr Phe Thr 1410 1415 1420 Ala Asp Lys Asp Val Ser lie Gin Pro Ser Pro Ser Phe Asp Glu Met 1425 1430 1435 1440 Asn Tyr Gin Phe Asn Ala Leu Glu lie Asp Gly Ser Gly Leu Asn Phe 1445 1450 1455 lie Asn Asn Ser Ala Ser lie Asp Val Thr Phe Thr Ala Phe Ala Glu 1460 1465 1470 (CNS) A4 specification (210X297 mm) (Please read the precautions on the back before filling this page) -107- 509722 A8 B8 C8 D8 Patent Application Range (Please read the notes on the back before filling this page) Asp Gly Arg Lys Leu Gly Tyr Glu Ser Phe Ser lie Pro Val Thr Leu 1475 1480 1485 Lys Val Ser Thr Asp Asn Ala Leu Thr Leu His His Asn Glu Asn Gly 1490 1495 1500 Ala Gin Tyr Met Gin Trp Gin Ser Tyr Arg Thr Arg Leu Asn Thr Leu 1505 1510 1515 1520 Phe Ala Arg Gin Leu Val Ala Arg Ala Thr Thr Gly lie Asp Thr lie 1525 1530 1535 Leu Ser Met Glu Thr Gin Asn lie Gin Glu Pro Gin Leu Gly Lys Gly 1540 1545 1550 Phe Tyr Ala Thr Phe Val lie Pro Pro Tyr Asn Leu Ser Thr His Gly 1555 1560 1565 Asp Glu Arg Trp Phe Lys Leu Tyr lie Lys His Val Val Asp Asn Asn 1570 1575 1580 Ser His lie lie Tyr Ser Gly Gin Leu Thr. Asp Thr Asn lie Asn lie 1585 1590 · 1595 1600 This paper size applies to China National Standard (CNS) A4 size (210X297 mm) -108- 509722 A8 B8 C8 D8 Patent Application Range Thr Leu Phe lie Pro Leu Asp Asp Val Pro Leu Asn Gin Asp Tyr His 1605 1610 .1615 Ala Lys Val Tyr Met Thr Phe Lys Lys Ser Pro Ser Asp Gly Thr Trp 1620 1625 1630 Trp Gly Pro His Phe Val Arg Asp Ass Lys Gly lie Val Thr lie Asn 1635 1640 1645 Pro Lys Ser lie Leu Thr His Phe Glu Ser Val Asn Val Leu Asn Asn 1650 1655 1660 lie Ser Ser Glu Pro Met Asp Phe Ser Gly Ala Asn Ser Leu Tyr Phe 1665 1670 1675 1680 Trp Glu Leu Phe Tyr Tyr Thr Pro Met Leu Val Ala Gin Arg Leu Leu 1685 1690 1695 His Glu Gin Asn Phe Asp Glu Ala Asn Arg Trp Leu Lys Tyr Trp 1700 1705 1710 Ser Pro Ser Gly Tyr lie Val His Gly Gin. Lie Gin Asn Tyr Gin Trp 1715 1720 1725 (Please read the notes on the back before filling out this page), -Ί · 聿 -109- This paper size applies to China National Standard (CNS) A4 Specification (210X297 mm) 509722 A8 B8 C8 D8 Patent Application Range (Please read the notes on the back before filling this page) Asn Val Arg Pro Leu Leu Glu Asp Thr Ser Trp Asn Ser Asp Pro Leu 1730 1735 1740 Asp Ser Val Asp Pro Asp Ala Val Ala Gin His Asp Pro Met His Tyr 1745 1750 1755 1760 Lys Val Ser Thr Phe Met Arg Thr Leu Asp Leu Leu lie Ala Arg Gly 17 65 1770 1775 Asp His Ala Tyr Arg Gin Leu Glu Arg Asp Thr Leu Asn Glu Ala Lys 1780 1785 1790 Met Trp Tyr Met Gin Ala Leu His Leu Leu Gly Asp Lys Pro Tyr Leu 1795 1800 1805 Pro Leu Ser Thr Thr Trp Ser Asp Pro Arg Leu Asp Arg Ala Ala Asp 1810 1815 1820 lie Thr Thr Gin Asn Ala His Asp Ser Ala lie Val Ala Leu Arg Gin 1825 1830 1835 1840 Asn lie Pro Thr Pro Ala Pro Leu Ser 1845 1849 Serial ID No. 51: This paper Standards are applicable to Chinese National Standard (CNS) A4 specifications (210X297 mm) -110 · 509722 A8 B8 C8 D8 VI. Patent application scope Leu Arg Ser Ala Asn Thr Leu Thr Asp Leu Phe Leu Pro Gin lie Asn 1 5 10 .15 Glu Val Met Met Asn Tyr Trp Gin Thr Leu Ala Gin Arg Val Tyr Asn 20 25 30 Leu Arg His Asn Leu Ser lie Asp Gly Gin Pro Leu Tyr Leu Pro lie 35 40 45 Tyr Ala Thr Pro Ala Asp Pro Lys Ala Leu Leu Ser Ala Ala Val Ala 50 55 60 Thr Ser Gin Gly Gly Gly Lys Leu Pro Glu Ser Phe Met Ser Leu Trp 65 70 75 80 Arg Phe Pro His Met Leu Glu Asn Ala Arg Gly Met Val Ser Gin Leu 85 90 9 5 Thr Gin Phe Gly Ser Thr Leu Gin Asn lie lie Glu Arg Gin Asp Ala 100 105 110 Glu Ala Leu Asn Ala Leu Leu Gin Asn Gin Ala Ala Glu Leu lie Leu 115 120 125 _____- 111- This paper size applies to Chinese national standards (CNS) A4 size (210 X 297 mm) (Please read the precautions on the back before filling this page) 509722 A8 B8 C8 D8 Application scope Thr Asn Leu Ser lie Gin Asp Lys Thr lie Glu Glu Leu Asp Ala Glu 13〇135 140 Lys Thr Val Leu Glu Lys Ser Lys Ala Gly Ala Gin Ser Arg Phe Asp 145 150 155 160 Ser Tyr Gly Lys Leu Tyr Asp Glu Asn lie Asn Ala Gly Glu Asn Gin 165 170 175 Ala Met Thr Leu Arg Ala Ser Ala Ala Gly Leu Thr Thr Ala Val Gin 180 185 190 Ala Ser Arg Leu Ala Gly Ala Ala Ala Asp Leu Val Pro Asn lie Phe 195 200 205 Gly Phe Ala Gly Gly Gly Gly Ser Arg Trp Gly Ala lie Ala Glu Ala Thr 210 215 220 Gly Tyr Val Met Glu Phe Ser Ala Asn Val Met Asn Thr Glu Ala Asp 225 230 235 240 Lys lie Ser Gin Ser Glu Thr Tyr Arg Arg Arg Arg Gin Glu Trp Glu 245 250 255 This paper size is applicable to Chinese National Standard (CNS) A4 size (210 X 297 mm) Order I line. (Please read the precautions on the back before filling this page)- 112- 509722 A8 B8 C8 D8 VI. Application scope lie Gin Arg Asn Asn Ala Glu Ala Glu Leu Lys Gin lie Asp Ala Gin 260 265 270 Leu Lys Ser Leu Ala Val Arg Arg Glu Ala Ala Val Leu Gi n Lys Thr 275 280 285 Ser Leu Lys Thr Gin Gin Glu Gin Thr Gin Ser Gin Leu Ala Phe Leu 290 295 300 Gin Arg Lys Phe Ser Asn Gin Ala Leu Tyr Asn Trp Leu Arg Gly Arg 305 310 315 320 Leu Ala Ala lie Tyr Phe Gin Phe Tyr Asp Leu Ala Val Ala Arg Cys 325 330 335 Leu Met Ala Glu Gin Ala Tyr Arg Trp Glu Leu Asn Asp Asp Ser Ala 340 345 350 Arg Phe lie Lys Pro Gly Ala Trp Gin Gly Thr Tyr Ala Gly Leu Leu 355 360 365 Ala Gly Glu Thr Leu Met Leu Ser Leu Ala. Gin Met Glu Asp Ala His 370 375 380 This paper size applies to China National Standard (CNS) A4 (210 X 297 mm) (Please read the precautions on the back before reading (Fill in this page) -113- 509722 A8 B8 C8 D8 Patent Application Range Leu Lys Arg Asp Lys Arg Ala Leu Glu Val G1 u Arg Thr Val Ser Leu 385 390 395 400 Ala Glu Val Tyr Ala Gly Leu Pro Lys Asp Asn Gly Pro Phe Ser Leu 405 410 415 Ala Gin Glu lie Asp Lys Leu Val Ser Gin Gly Ser Gly Ser Ala Gly 420 425 430 Ser Gly Asn Asn Asn Leu Ala Phe Gly Ala Gly Thr Asp Thr Lys Thr 435 440 445 Ser Leu Gin Ala Ser Val Ser Phe Ala Asp Leu Lys lie Arg Glu Asp 450 455 460 Tyr Pro Ala Ser Leu Gly Lys lie Arg Arg lie Lys Gin lie Ser Val 465 470 475 480 Thr Leu Pro Ala Leu Leu Gly Pro Tyr Gin Asp Val Gin Ala lie Leu 485 490 495 Ser Tyr Gly Asp Lys Ala Gly Leu Ala Asn Gly Cys Glu Ala Leu Ala 500 505 510 This paper size applies to the Chinese National Standard (CNS) M specification (210X297 mm) (Please read the precautions on the back before filling this page) -114- 509722 A8 B8 C8 D8 VI. Patent application scope Val Ser His Gly Met Asn Asp Ser Gly Gin Phe Gin Leu Asp Phe Asn 515 520 525 Asp Gly Lys Phe Leu Pro Phe Glu Gly lie Ala lie Asp Gin Gl y Thr 530 535 540 Leu Thr Leu Ser Phe Pro Asn Ala Ser Met Pro Glu Lys Gly Lys Gin 545 550 555 560 Ala Thr Met Leu Lys Thr Leu Asn Asp lie lie Leu His lie Arg Tyr 565 570 575 Thr 11e Lys *** 579 Sequence ID No. 53: Phe lie Gin Gly Tyr Ser Asp Leu Phe Gly Asn Arg Ala Asp Asn Tyr 15 10 15 Ala Ala Pro Gly Ser Val Ala Ser Met Phe Ser Pro Ala Ala Tyr Leu 20 25 30 Thr Glu Leu Tyr Arg Glu Ala Lys Asn Leu.His Asp Ser Ser Ser lie 35 40 45 ____; _-115- This paper size applies to China National Standard (CNS) A4 (210 X 297 mm) (Please read the precautions on the back before (Fill in this page) Order-509722 A8 B8 C8 D8 6. Application scope Tyr Tyr Leu Asp Lys Arg Arg Pro Asp Leu Ala Ser Leu Met Leu Ser 50 55 60 Gin Lys Asn Met Asp Glu Glu lie Ser Thr Leu Ala Leu Ser Asn Glu 65 70 75 80 Leu Gys Leu Ala Gly lie Glu Thr Lys Thr Gly Lys Ser Gin Asp Glu 85 90 95 Val Met Asp Met Leu Ser Thr Tyr Arg Leu Ser Gly Glu Thr Pro Tyr 100 105 110 His His Ala Tyr Glu Thr Val Arg Glu lie Val His Glu Arg Asp Pro 115 120 125 Gly Phe Arg His Leu Ser Gin Ala Pro lie Val Ala Ala Lys Leu Asp 130 135 140 Pro Val Thr Leu Leu Gly lie Ser Ser His lie Ser Pro Glu Leu Tyr 145 150 155 160 Asn Leu Leu lie Glu Glu lie Pro Glu Lys. Asp Glu Ala Ala Leu Asp 165 170 175 This paper size applies to the Chinese National Standard (CNS) A4 specification (210 X 297 mm) ........—... ............., installed ... (Please read the notes on the back before filling this page) -116- 509722 A8 B8 C8 D8 VI. Patent Application Range Thr Leu Tyr Lys Thr Asn Phe Gly Asp lie Thr Thr Ala Gin Leu Met 180 185 190. Ser Pro Ser Tyr Leu Ala Arg Tyr Tyr Gly Val Ser Pro Glu Asp lie 195 200 205 Ala Tyr Val Thr Thr Ser Leu Ser His Val Gly Tyr Ser Ser Asp lie 210 215 220 Leu Val lie Pro Leu Val Asp Gly Val Gly Lys Met Glu Val Val Arg 225 230 235 240 Val Thr Arg Thr Pro Ser Asp Asn Tyr Thr Ser Gin Thr Asn Tyr lie 245 250 255 Glu Leu Tyr Pro Gin Gly Gly Asp Asn Tyr Leu lie Lys Tyr Asn Leu 260 265 27 0 Ser Asn Ser Phe Gly Leu Asp Asp Phe Tyr Leu Gin Tyr Lys Asp Gly 275 280 285 Ser Ala Asp Trp Thr Glu lie Ala His AsnPro Tyr Pro Asp Met Val 290 295 300 This paper size is in accordance with China National Standard (CNS) A4 specifications (21〇X297 mm) (Please read the notes on the back before filling this page) -117- 509722 A8 B8 C8 D8 Patent Application Range lie Asn Gin Lys Tyr Glu Ser Gin Ala Thr lie Lys Arg Ser Asp Ser 305 310 315 320 Asp Asn lie Leu Ser lie Gly Leu Gin Arg Trp His Ser Gly Ser Tyr 325 330 335 Asn Phe Ala Ala Ala Asn Phe Lys lie Asp Gin Tyr Ser Pro Lys Ala 340 345 350 Phe Leu Leu Lyu Met Asn Lys Ala lie Arg Leu Leu Lys Ala Thr Gly 355 360 365 Leu Ser Phe Ala Thr Leu Glu Arg lie Val Asp Ser Val Asn Ser Thr 370 375 380 Lys Ser lie Thr Val Glu Val Leu Asn Lys Val Tyr Arg Val Lys Phe 385 390 395 400 Tyr lie Asp Arg Tyr Gly lie Ser Glu Glu Thr Ala Ala lie Leu Ala 405 410 415 Asn lie Asn lie Ser Gin Gin Ala Val Gly.Asn Gin Leu Ser Gin Phe 420 425 430 This paper size applies to China National Standard (CNS) A4 (210X297 mm) ----------------- ----- S ........., 玎 ............ 绛 (Please read the back first Please pay attention to this page and fill in this page) -118- 509722 A8 B8 C8 D8 Patent Application Range Glu Gin Leu Phe Asn His Pro Pro Leu Asn Gly lie Arg Tyr Glu lie 435 440 44 5 Ser Glu Asp Asn Ser Lys Hi s Leu Pro Asn Pro Asp Leu Asn Leu Lys 450 455 460 Pro Asp Ser Thr Gly Asp Asp Gin Arg Lys Ala Val Leu Lys Arg Ala 465 470 475 480 Phe Gin Val Asn Ala Ser Glu Leu Tyr Gin Met Leu Leu lie Thr Asp 485 490 495 Arg Lys Glu Asp Gly Val lie Lys Asn Asn Leu Glu Asn Leu Ser Asp 500 505 510 Leu Tyr Leu Val Ser Leu Leu Ala Gin lie His Asn Leu Thr lie Ala 515 520 525 Glu Leu Asn lie Leu Leu Val lie Cys Gly Tyr Gly Asp Thr Asn lie 530 535 540 Tyr Gin lie Thr Asp Asp Asn Leu Ala Lys.lie Val Glu Thr Leu Leu 545 550 _ 555 560 This paper is dimensioned to the Chinese National Standard (CNS) A4 Specifications (210X297mm) (Please read the notes on the back before filling this page) -119- 509722 A8 B8 C8 D8 Patent Application Fan Park Trp lie Thr Gin Trp Leu Lys Thr Gin Lys Trp Thr Val Thr Asp Leu 565 570 575 Phe Leu Met Thr Thr Ala Thr Tyr Ser Thr Thr Leu Thr Pro Glu lie 580 585 590 Ser Asn Leu Thr Ala Thr Leu Ser Ser Thr Leu His Gly Lys Glu Ser 595 600 605 Leu lie Gly Glu Asp Leu Lys Arg Ala Met Ala Pro Cys Phe Thr Ser 610 615 620 Ala Leu His Leu Thr Ser Gin Glu Val Ala Tyr Asp Leu Leu Leu Trp 625 630 635, 640 lie Asp Gin lie Gin Pro Ala Gin lie Thr Val Asp Gly Phe Trp Glu 645 650 655 Glu Val Gin Thr Thr Pro Thr Ser Leu Lys Val lie Thr Phe Ala Gin 660 665 670 Val Leu Ala Gin Leu Ser Leu lie Tyr Arg Arg lie Gly Leu Ser Glu 675 680 685 (Please read the notes on the back before filling out this page) • Order ·: Line 丨 This paper size applies to China National Standard (CNS) A4 specification (210X297) -120- 509722 A8 B8 C8 D8 Patent Application Range Thr Glu Leu Ser Leu lie Val Thr Gin Ser Ser Leu Leu Val Ala Gly 690 695 700 Lys Ser lie Leu Asp His Gly Leu Leu Thr Leu Met Ala Leu Glu Gly 705 710 715 720 Phe His Thr Trp Val Asn Gly Leu Gly Gin His Ala Ser Leu lie Leu 725 730 735 Ala Ala Leu Lys Asp Gly Ala Leu Thr Val Thr Asp Val Ala Gin Ala 740 745 750 Met Asn Lys Glu Glu Ser Leu Leu Gin Met Ala Ala Asn Gin Val Glu 755 760 765 Lys Asp Leu Thr Lys Leu Thr Ser Trp Thr Gin lie Asp Ala lie Leu 770 775 780 Gin Trp Leu Gin Met Ser Ala Leu Ala Val Ser Pro Leu Asp Leu 785 790 795 800 Ala Gly Met Met Ala Leu Lys Tyr Gly lie Asp His Asn Tyr Ala Ala 805 丨 810 815 This paper size applies to China National Standard (CNS) A4 (210X297 mm) (Please read the precautions on the back before filling this page) 、-丨 丨 -121- 509722 A8 B8 C8 D8 VI.Application scope Trp Gin Ala Ala Ala Ala Ala Ala Leu Met Ala Asp His Ala Asn Gin Ala 820 825 830 Gin Lys Lys Leu Asp Glu Thr Phe Ser Lys Ala Leu Cys Asn Tyr Tyr 835 840 845 lie Asn Ala Val Val Asp Ser Ala Ala Gly Val Arg Asp Arg Asn Gly 850 855 860 Leu Tyr Thr Tyr Leu Leu lie Asp Asn Gin Val Ser Ala Asp Val lie 865 870 875 880 Thr Ser Arg lie Ala Glu Ala lie Ala Gly lie Gin Leu Tyr Val Asn 885 890 895 Arg Ala Leu Asn Arg Asp Glu Gly Gin Leu Ala Ser Asp Val Ser Thr 900 905 910 Arg Gin Phe Phe Thr Asp Trp Glu Arg Tyr Asn Lys Arg Tyr Ser Thr 915 920 925 Trp Ala Gly Val Ser Glu Leu Val Tyr Tyr Pro Glu Asn Tyr Val Asp 930 935 940 (Please read the notes on the back before filling out this page) Order: Line 丨 -122- This paper size applies China National Standard (CNS) A4 specification (210X297 mm) 509722 A8 B8 C8 D8 VI. Patent application scope Pro Thr Gin Arg lie Gly Gin Thr Lys Met Met Asp Ala Leu Leu Gin 945 950 955 960 Ser lie Asn Gin Ser Gin Leu Asn Ala Asp Thr Val Glu Asp Ala Phe 965 970 970 Lys Thr Tyr Leu Thr Ser Phe Glu Gin Val Ala Asn Leu Lys Val lie 980 985 990 Ser Ala Tyr His Asp Asn Val Asn Val Asp Gin Gly Leu Thr Tyr Phe 995 1000 1005 lie Gly lie Asp Gin Ala Ala Pro Gly Thr Tyr Tyr Trp Arg Ser Val 1010 1015 1020 Asp His Ser Lys Cys Glu Asn Gly Lys Phe Ala Ala Asn Ala Trp Gly 1025 1030 1035 1040 Glu Trp Asn Lys lie Thr Cys Ala Val Asn Pro Trp Lys Asn lie lie 1045 1050 1055 Arg Pro Val Val Tyr Met Ser Arg Leu Tyr Leu Leu Trp Leu Glu Gin 1060 1065 1070 This paper size applies to China National Standard (CNS) A4 specifications (210X 297 Mm) (Please read the notes on the back before filling out this page) -123- 509722 A8 B8 C8 D8 patent application scope (please read the notes on the back before filling this page) Gin Ser Lys Lys Ser Asp Asp Gly Lys Thr Thr lie Tyr Gin Tyr Asn 1075 1080 1085 Leu Lys Leu Ala His lie Arg Tyr Asp Gly Ser Trp Asn Thr Pro Phe 1090 1095 Π00 Thr Phe Asp Val Thr Glu Lys Val Lys Asn Tyr Thr Ser Ser Thr Asp 1105 1110 1115 1120 Ala Ala Glu Ser Leu Gly Leu Tyr Cys Thr Gly Tyr Gin Gly Glu Asp 1125 1130 1135 Thr Leu Leu Val Met Phe Tyr Ser Met Gin Ser Ser Tyr Ser Ser Tyr 1140 1145 Π50 Thr Asp Asn Asn Ala Pro Val Thr Gly Leu Tyr lie Phe Ala Asp Met 1155 1160 1165 Ser Ser Asp Asn Met Thr Asn Ala Gin Ala Thr Asn Tyr Trp Asn Asn 1170 1175 1180 Ser Tyr Pro Gin Phe Asp Thr Val Met Ala Asp Pro Asp Ser Asp Asn 1185 1190. 1195 1200 -124- This paper size applies to China National Standard (CNS) A4 (210X297 mm) 509722 A8 B8 C8 D8 Patent scope Lys Lys Val lie Thr Arg Arg Val Asn Asn Arg Tyr Ala Glu Asp Tyr 1205 1210 .1215 Glu lie Pro Ser Ser Val Thr Ser Asn Ser Asn Tyr Ser Trp Gly Asp 1220 1225 1230 His Ser Leu Thr Met Leu Tyr Gly Gly Ser Val Pro Asn lie Thr Phe 1235 1240 1245 Glu Ser Ala Ala Glu Asp Leu Arg Leu Ser Thr Asn Met Ala Leu Ser 1250 1255 1260 lie lie His Asn Gly Tyr Ala Gly Thr Arg Arg lie Gin Cys Asn Leu 1265 1270 1275 1280 Met Lys Gin Tyr AlaSer Leu Gly Asp Lys Phe lie lie lie Tyr Asp Ser 1285 1290 1295 Ser Phe Asp Asp Ala Asn Arg Phe Asn Leu Val Pro Leu Phe Lys 1300 1305 1310 Gly Lys Asp Glu Asn Ser Asp Asp Ser lie Cys He Tyr Asn Glu Asn 1315 1320 1325 This paper size applies the Chinese National Standard (CNS) A4 specification (210X297 mm) (Please read the precautions on the back before filling in this Page)-, '^ 丨 -125- 509722 A8 B8 C8 D8 VI. Patent Application Pro Ser Ser Glu Asp Lys Lys Trp Tyr Phe Ser Ser Lys Asp Asp Asn 1330 1335 1340 Lys Thr Ala Asp Tyr Asn Gly Gly Thr Gin Cys lie Asp Ala Gly Thr 1345 1350 1355 1360 Ser Asn Lys Asp Phe Tyr Tyr Asn Leu Gin Glu lie Glu Val lie Ser 1365 1370 1375 Val Thr Gly Gly Tyr Trp Ser Ser Tyr Lys lie Ser Asn Pro lie Asn 1380 1385 1390 lie Asn Thr Gly lie Asp Ser Ala Lys Val Lys Val Thr Val Lys Ala 1395 1400 1405 Gly Gly Asp Asp Gin lie Phe Thr Ala Asp Asn Ser Thr Tyr Val Pro 1410 1415 1420 Gin Gin Pro Ala Pro Ser Phe Glu Glu Met lie Tyr Gin Phe Asn Asn 1425 1430 1435 1440 Leu Thr lie Asp Cys Lys Asn Leu Asn Phe lie Asp Asn Gin Ala His 1445 1450 1455 X 297 mm) (Please read the precautions on the back before filling in this page) ▼, -P 丨: Line · -126- 509722 A8 B8 C8 D8 VI. Application scope lie Glu lie Asp Phe Thr Ala Thr Ala Gin Asp Gly Arg Phe Leu Gly 1460 1465 1470. Ala Glu Thr Phe lie lie Pro Val Thr Lys Lys Val Leu Gly Thr Glu 1475 1480 1485 Asn Val lie Ala Leu Tyr Ser Glu Asn Gly Val Gin Tyr Met Gin 1490 1495 1500 lie Gly Ala Tyr Arg Thr Arg Leu Asn Thr Leu Phe Ala Gin Gin Leu 1505 1510 1515 1520 Val Ser Arg Ala Asn Arg Gly lie Asp Ala Val Leu Ser Met Glu Thr 1525 1530 1535 Gin Asn lie Gin GluPro Gin Leu Gly Ala Gly Thr Tyr Val Gin Leu 1540 1545 1550 Val Leu Asp Lys Tyr Asp Glu Ser lie His Gly Thr Asn Lys Ser Phe 1555 1560 1565 Ala lie Glu Tyr Val Asp lie Phe Lys Glu Asn Asp Ser Phe Val lie 1570 1575 1580 This paper size is in accordance with Chinese National Standards (CNS) A4 (210 X 297 mm) (Please read the precautions on the back before filling out this page). Range Tyr Gin Gly Glu Leu Ser Glu Thr Ser Gin Thr Val Val Lys Val Phe (Please read the notes on the back before filling this page) 1585 1590 1595 1600 Leu Ser Tyr Phe lie Glu Ala Thr Gly Asn Lys Asn His Leu Trp Val 1605 1610 1615 Arg Ala Lys Tyr Gin Lys Glu Thr Thr Asp Lys lie Leu Phe Asp Arg 1620 1625 1630 Thr Asp Glu Lys Asp Pro His Gly Trp Phe Leu Ser Asp Asp His Lys 1635 1640 1645 Thr Phe Ser Gly Leu Ser Ser Ala Gin Ala Leu Lys Asn Asp Ser Glu 1650 1655 1660 Pro Met Asp Phe Ser Gly Ala Asn Ala Leu Tyr Phe Trp Glu Leu Phe 1665 1670 1675 1680 Tyr Tyr Thr Pro Met Met Met Ala His Arg Leu Leu Gin Glu Gin Asn 1685 1690 1695 Phe Asp Ala Ala Asn His Trp Phe Arg Tyr. Val Trp Ser Pro Ser Gly 1700 1705 1710 -128- This paper size applies to China National Standard (CNS) A4 (210X 297 mm) ) 509722 A8 B8 C8 D8 patent application scope Tyr lie Val Asp Gly Lys lie Ala lie Tyr His Trp Asn Val Arg Pro 1715 1720 1725 Leu Glu Glu Asp Thr Ser Trp Asn Ala Gin Gin Leu Asp Ser Thr Asp 1730 1735 1740 Pro Asp Ala Val Ala Gin Asp Asp Pro Met His Tyr Lys Val Ala Thr 1745 1750 1755 Π60 Phe Met Ala Thr Leu Asp Leu Leu Met Ala Arg Gly Asp Ala Ala Tyr 1765 1770 1775 Arg Gin Leu Glu Arg Asp Thr Leu Ala Glu Ala Lys Met T Tyr Thr 1780 1785 1790 Gin Ala Leu Asn Leu Leu Gly Asp Glu Pro Gin Val Met Leu Ser Thr 1795 1800 1805 Thr Trp Ala Asn Pro Thr Leu Gly Asn Ala Ala Ser Lys Thr Thr Gin 1810 1815 1820 Gin Val Arg Gin Gin Val Leu Thr Gin Leu Arg Leu Asn Ser Arg Val 1825 1830 · 1835 1840 This paper size is applicable to China National Standard (CNS) A4 (210X297 mm) (Please read first Note the surface to fill out this page) -129 · 509722 A8 B8 C8 D8 Patent Application Scope (Please read the notes on the back before filling this page) Lys Thr Pro Leu 1844 Serial Identification Number 55: Leu Gly Thr Ala Asn Ser Leu Thr Ala Leu Phe Leu Pro Gin Glu Asn 1 5 10 15 Ser Lys Leu Lys Gly Tyr Trp A'rg Thr Leu Ala Gin Arg Met Phe Asn 20 25 30 Leu Arg His Asn Leu Ser lie Asp Gly Gin Pro Leu Ser Leu Pro Leu 35 40 45 Tyr Ala Lys Pro Ala Asp Pro Lys Ala Leu Leu Ser Ala Ala Val Ser 50 55 60 Ala Ser Gin Gly Gly Ala Asp Leu Pro Lys Ala Pro Leu Thr lie His 65 70 75 80 Arg Phe Pro Gin Met Leu Glu Gly Ala Arg Gly Leu Val Asn Gin Leu 85 90 95 lie Gin Phe Gly Ser Ser Leu Leu Gly Tyr Ser Glu Arg Gin Asp Ala 100 105 110 Glu Ala Met Ser Gin Leu Leu Gin Thr Gin Ala Ser Glu Leu lie Leu This paper size applies to Chinese National Standard (CNS) A4 Specifications (210 X 297 mm) -130- 509722 A8 B8 C8 D8 VI. Patent Application Range 115 120 125 Thr Ser lie Arg Met Gin Asp Asn Gin Leu Ala Glu Leu Asp Ser Glu 130 135 140 Lys Thr Ala Leu Gin Val SerLeu Ala Gly Val Gin Gin Arg Phe Asp 145 150 155 160 Ser Tyr Ser Gin Leu Tyr Glu Glu Asn lie Asn Ala Gly Glu Gin Arg 165 170 175 Ala Leu Ala Leu Arg Ser Glu Ser Ala lie Glu Ser Gin Gly Ala Gin 180 185 190 lie Ser Arg Met Ala Gly Ala Gly Val Asp Met Ala Pro Asn lie Phe 195 200 205 Gly Leu Ala Asp Gly Gly Met His Tyr Gly Ala lie Ala Tyr Ala lie 210 215 220 Ala Asp Gly lie Glu Leu Ser Ala Ser Ala Lys Met Val Asp Ala Glu 225 230 235 240 Lys Val Ala Gin Ser Glu lie Tyr Arg Arg Arg Arg Gin Glu Trp Lys This paper size applies to Chinese National Standards (CNS) Μ specifications (210 X 297 mm) (Please read the back (Please fill in this page again) -131- 509722 A8 B8 C8 D8 VI. Patent Application Range 245 250 255 (Please read the notes on the back before filling this page) lie Gin Arg Asp Asn Ala Gin Ala Glu lie Asn Gin Leu Asn Ala Gin 260 265 270 Leu Glu Ser Leu Ser lie Arg A'rg Glu Ala Ala Glu Met Gin Lys Glu 275 280 285 Tyr Leu Lys Thr Gin Gin Ala Gin Ala Gin Ala Gin Leu Thr Phe Leu 290 295 300 Arg Ser Lys Phe Ser Asn Gin Ala Leu Tyr Ser Trp Leu Arg Gly Arg 305 310 315 320 Leu Ser Gly lie Tyr Phe Gin Phe Tyr Asp Leu Ala Val Ser Arg Cys 325 · 330 335 Leu Met Ala Glu Gin Ser Tyr Gin Trp Glu Ala Asn Asp Asn Ser lie 340 345 350 Ser Phe Val Lys Pro Gly Ala Trp Gin Gly Thr Tyr Ala Gly Leu Leu 355 360 365 Cys Gly Glu Ala Leu lie Gin Asn Leu Ala Gin Met Glu Glu Ala Tyr This paper size applies the Chinese National Standard (CNS) A4 specification (210X297 mm)- 132- 509722 A8 B8 C8 D8 Patent Application Range 370 375 380 Leu Lys Trp Glu Ser Arg Ala Leu Glu Val Glu Arg Thr Val Ser Leu 385 390 395 400 Ala Val Val Tyr Asp Ser Leu Glu Gly Asn Asp Arg Phe Asn Leu Ala 405 410 415 Glu Gin lie Pro Ala Leu Leu Asp Lys Gly Glu Gly Thr Ala Gly Thr 420 425 430 Lys Glu Asn Gly Leu Ser Leu Ala Asn Ala lie Leu Ser Ala Ser Val 435 440 445 Lys Leu Ser Asp Leu Lys Leu Gly Thr Asp Tyr Pro Asp Ser lie Val 450 455 460 Gly Ser Asn Lys Val Arg Arg lie Lys Gin lie Ser Val Ser Leu Pro 465 470 475 480 Ala Leu Val Gly Pro Tyr Gin Asp Val Gin Ala Met Leu Ser Tyr Gly 485 490 495 Gly Ser Thr Gin Leu Pro Lys Gly Cys Ser Ala Leu Ala Val Ser His (Please read the precautions on the back before filling this page) ·, >! t -133- This paper size applies to Chinese national standards (CNS) A4 specification (210 X 297 mm) 509722 A8 B8 C8 D8 Patent application scope 500 505 510 (Please read the notes on the back before filling this page) Gly Thr Asn Asp Ser Gly Gin Phe Gin Leu Asp Phe Asn Asp Gly Lys 515 520 525 Tyr Leu Pro Phe Glu Gly lie Ala Leu Asp Asp Gin Gly Thr Leu Asn 530 535 540 Leu Gin Phe Pro Asn Ala Thr Asp Lys Gin Lys Ala lie Leu Gin Thr 545 550 555 560 Met Ser Asp lie lie Leu His lie Arg Tyr Thr lie Arg *** 565 570 573 Sequence identification number 57: 1 Met Asn Gin Leu Ala Ser Pro Leu lie Ser Arg Thr Glu Glu lie His 16 17 Asn Leu Pro Gly Lys Leu Thr Asp Leu Gly Tyr Thr Ser Val Phe Asp 32 33 Val Val Arg Met Pro Arg Glu Arg Phe lie Arg Glu His Arg Ala Asp 48 49 Leu Gly Arg Ser Ala Glu Lys Met Tyr Asp Leu Ala Val Gly Tyr Ala 64 65 His Gin Val Leu His His Phe Arg Arg Asn Ser Leu Ser Glu Ala Val 80 -134- This paper size applies Chinese National Standard (CNS) A4 specification (210 X 297 mm) 509722 A8 B8 C8 D8 6. Application scope 81 Gin Phe Gly Leu Arg Ser Pro Phe Ser Val Ser Gly Pro Asp Tyr Ala 96 97 Asn Gin Phe Leu Asp Ala Asn Thr Gly Trp Lys Asp Lys Ala Pro Ser 112 113 Gly Ser Pro Glu Ala Asn Asp Ala Pro Val Ala Tyr Leu Thr His lie 128 129 Tyr Gin Leu Ala Leu Glu Gin Glu Lys Asn Gly Ala Thr Thr lie Met 144 145 Asn Thr Leu Ala Glu Arg Arg Pro Asp Leu Gly Ala Leu Leu lie Asn 160 161 Asp Lys Ala lie Asn Glu Val lie Pro Gin Leu Gin Leu Val Asa Glu 176 177 lie Leu Ser Lys Ala lie Gin Lys Lyu Leu Ser Leu Thr Asp Leu Glu 192 193 Ala Val Asn Ala Arg Leu Ser Thr Thr Arg Tyr Pro Asn Asn Leu Pro 208 209 Tyr His Tyr Gly His Gin Gin lie Gin Thr Ala Gin Ser Val Leu Gly 224 225 Thr Thr Leu Gin Asp lie Thr Leu Pro Gin Thr Leu Asp Leu Pro Gin 240 241 Asn Phe Trp Ala Thr Ala Lys Gly Lys Leu Ser Asp Thr Thr Ala Ser 256 257 Ala Leu Thr Arg Leu Gin lie Met Ala Ser Gin Phe Ser Pro Glu Gin 272 This paper size applies to Chinese National Standard (CNS) A4 (210 X 297 mm) (Please read the precautions on the back before filling this page) -135- 509722 A8 B8 C8 D8 Patent Application Range 273 Gin Lys lie lie Thr Glu Thr Val Gly Gin Asp Phe Tyr Gin Leu Asn 288 289 Tyr Gly Asp Ser Ser Leu Thr Val Asn Ser Phe Ser Asp Met Thr lie 304 305 Met Thr Asp Arg Thr Ser Leu Thr Val Pro Gin Val Glu Leu Met Leu 320 321 Cys Ser Thr Val Gly Gly Ser Thr Val Val Lys Ser Asp Asn Val Ser 336 337 Ser Gly Asp Thr Thr Ala Thr Pro Phe Ala Tyr Gly Ala Arg Phe lie 352 353 His Ala Gly Lys Pro Glu Ala lie Thr Leu Ser Arg Ser Gly Ala Glu 368 369 Ala His Phe Ala Leu Thr Val Asn Asn Leu Thr Asp Asp Lys Leu Asp 384 385 Arg lie Asn Arg Thr Val Arg Leu Gin Lys Trp Leu Asn Leu Pro Tyr 400 401 Glu Asp lie Asp Leu Leu Val Thr Ser Ala Met Asp Ala Glu Thr Gly 416 417 Asn Thr Ala Leu Ser Met Asn Asp Asn Thr Leu Arg Met Leu Gly Val 432 433 Phe Lys His Tyr Gin Ala Lys Tyr Gly Val Ser Ala Lys Gin Phe Ala 448 449 Gly Trp Leu Arg Val Va.l Ala Pro Phe Ala lie Thr Pro Ala Thr Pro 464 -136- This paper size applies to China National Standard (CNS) A4 (210 X 297 mm) ). ................. tr ............. ........ line. (Please read the precautions on the back before filling this page) 509722 A8 B8 C8 D8 VI. Patent Application Range 465 Phe Leu Asp Gin Val Phe Asn Ser Val Gly Thr Phe Asp Thr Pro Phe 480 481 Val lie Asp Asn Gin Asp Phe Val Tyr Thr Leu Thr Thr Gly Gly Asp 496 497 Gly Ala Arg Val Lys His lie Ser Thr Ala Leu Gly Leu Asn His Arg 512 513 Gin Phe Leu Leu Leu Leu Ala Asp Asn lie Ala Arg Gin Gin Gly Asn Val 528 529 Thr Gin Ser Thr Leu Asn Cys Asn Leu Phe Val Val Ser Ala Phe Tyr 544 545 Arg Leu Ala Asn Leu Ala Arg Thr Leu Gly lie Asn Pro Glu Ser Phe 560 561 Cys Ala Leu Val Asp Arg Leu Asp Ala Gly Thr Gly lie Val Trp Gin 576 577 Gin Leu Ala Gly Lys Pro Thr lie Thr Val Pro Gin Lys Asp Ser Pro 592 593 Leu Ala Ala Asp lie Leu Ser Leu Leu Gin Ala Leu Ser Ala lie Ala 608 609 Gin Trp Gin Gin Gin His Asp Leu Glu Phe Ser Ala Leu Leu Leu Leu 624 625 Leu Ser Asp Asn Pro lie Ser Thr Ser Gin Gly Thr Asp Asp Gin Leu 640 641 Asn Phe lie Arg Gin Va-1 Trp Gin Asn Leu Gly Ser Thr Phe Val Gly 656 This paper size applies to Chinese National Standard (CNS) A4 (210X297 mm) (Please read the precautions on the back before filling this page) -137- 509722 A8 B8 C8 D8 Patent Application Range 657 Ala Thr Leu Leu Ser Arg Ser Gly Ala Pro Leu Val Asp Thr Asn Gly 672 (Please read the precautions on the back before filling this page) 673 His Ala lie Asp Trp Phe Ala Leu Leu Ser Ala Gly Asn Ser Pro Leu 688 689 lie Asp Lys Val Gly Leu Val Thr Asp Ala Gly lie Gin Ser Val lie 704 705 Ala Thr Val Val Asn Thr Gin Ser Leu Ser Asp Glu Asp Lys Lys Leu 720 721 Ala lie Thr Thr Leu Thr Asn Thr Leu Asn Gin Val Gin Lys Thr Gin 736 737 Gin Gly Val Ala Val Ser Leu Leu Ala Gin Thr Leu Asn Val Ser Gin 752 753 Ser Leu Pro Ala Leu Leu Leu Arg Trp Ser Gly Gin Thr Thr Tyr Gin 768 769 Trp Leu Ser Ala Thr Trp Ala Leu Lys Asp Ala Val Lys Thr Ala Ala 784 785 Asp lie Pro Ala Asp Tyr Leu Arg Gin Leu Arg Glu Val Val Arg Arg 800 801 Ser Leu Leu Thr Gin Gin Phe Thr Leu Ser Pro Ala Met Val Gin Thr 816 817 Leu Leu Asp Tyr Pro Ala Tyr Phe Gly Ala Ser Ala Glu Thr Val Thr 832 833 Asp lie Ser Leu Trp Met Leu Tyr Thr Leu Ser Cys Tyr Ser Asp Leu 848 Standard (CNS) A4 specification (210 X 297 mm) -138- 509722 A8 B8 C8 D8 VI. Patent application scope 849 Leu Leu Gin Met Gly Glu Ala Gly Gly Thr Glu Asp Asp Val Leu Ala 864 (Please read the first Please fill in this page again) 865 Tyr Leu Arg Thr Ala Asn Ala Thr Thr Pro Leu Ser Gin Ser Asp Ala 880 881 Ala Gin Thr Leu Ala Thr Leu Leu Gly Trp Glu Val Asn Glu Leu Gin 896 897 Ala Ala Trp Ser Val Leu Gly Gly lie Ala Lys Thr Thr Pro Gin Leu 912 913 Asp Ala Leu Leu Arg Leu Gin Gin Ala Gin Asn Gin Thr Gly Leu Gly 928 929 Val Thr Gin Gin Gin Gin Gly Tyr Leu Leu Ser Arg Asp Ser Asp Tyr 944 945 Thr Leu Trp Gin Ser Thr Gly Gin Ala Leu Val Ala Gly Val Ser His 960 961 Val Lys Giy Ser Asn 965 Sequence Identification Number 59: 1 Met Leu Ser Thr Met Glu Lys Gin Leu Asn Glu Ser Gin Arg Asp Ala 16 17 Leu Val Thr Gly Tyr Met Asn Phe Val Ala Pro Thr Leu Lys Gly Val 32 33 Ser Gly Gin Pro Val Thr Val Glu Asp Leu Tyr Glu Tyr Leu Leu lie 48 49 Asp Pro Glu Val Ala Asp Glu Val Glu Thr Ser Arg Val Ala Gin Ala 64 ----------- 139- This paper size applies to Chinese National Standard (CNS) A4 specification (210X297 mm) 509722 A8 B8 C8 D8 Patent scope 65 Lie Ala Ser He Gin Gin Tyr Met Thr Arg Leu Val Asn Gly Ser Glu 80 81 Pro Gly Arg Gin Ala Met Glu Pro Ser Thr Ala Asn Glu Trp Arg Asp 96 97 Asn Asp Asn Gin Tyr Ala lie Trp Ala Ala Gly Ala Glu Val Arg Asn 112 113 Tyr Ala Glu Asn Tyr lie Ser Pro lie Thr Arg Gin Glu Lys Ser His 128 129 Tyr Phe Ser Glu Leu Glu Thr Thr Leu Asn Gin Asn Arg Leu Asp Pro 144 145 Asp Arg Val Gin Asp Ala Val Leu Ala Tyr Leu Asn Glu Phe Glu Ala 160 161 Val Ser Asn Leu Tyr Val Leu Ser Gly Tyr lie Asn Gin Asp Lys Phe 176 177 Asp Gin Ala lie Tyr Tyr Phe lie Gly Arg Thr Thr Thr Thr Lys Pro Tyr 192 193 Arg Tyr Tyr Trp Arg Gin Met Asp Leu Ser Lys Asn Arg Gin Asp Pro 208 209 Ala Gly Asn Pro Val Thr Pro Asn Cys Trp Asn Asp Trp Gin Glu lie 224 225 Thr Leu Pro Leu Ser Gly Asp Thr Val Leu Glu His Thr Val Arg Pro 240 241 Val Phe Tyr Asn Asp Arg Leu Tyr Val Ala Trp Val Glu Arg Asp Pro 256 This paper size is applicable to China National Standard (CNS) A4 specification (210X297 mm) .. ......... Order .....-......... line. (Please read the notes on the back before filling out this page) -140- 509722 A8 B8 C8 D8 VI. Application scope 257 Ala Val Gin Lys Asp Ala Asp Giy Lys Asn lie Gly Lys Thr His Ala 272. 273 Tyr Asn lie Lys Phe Gly Tyr Lys Arg Tyr Asp Asp Thr Trp Thr Ala 288 289 Pro Asn Thr Thr Thr Leu Met Thr Gin Gin Ala Gly Glu Ser Ser Glu 304 305 Thr Gin Arg Ser Ser Leu Leu lle Asp Glu Ser Ser Thr Thr Leu Arg 320 321 Gin Val Asn Leu Leu Ala Thr Thr Asp Phe Ser lie Asp Pro Thr Glu 336 337 Glu Thr Asp Ser Asn Pro Tyr Gly Arg Leu Met Leu Gly Val Phe Val 352 353 Arg Gin Phe Glu Gly Asp Gly Ala Asn Arg Lys Asn Lys Pro Val Val 368 369 Tyr Gly Tyr Leu Tyr Cys Asp Ser Ala Phe Asn Arg His Val Leu Arg 384 385 Pro Leu Ser Lys Asn Phe Leu Phe Ser Thr Tyr Arg Asp Glu Thr Asp 400 401 Gly Gin Asn Ser Leu Gin Phe Ala Val Tyr Asp Lys Lys Tyr Val lie 416 417 Thr Lys Val Val Thr Gly Ala Thr Glu Asp Pro Glu Asn Thr Gly Trp 432 433 Val Ser Lys Val Asp Asp Leu Lys Gin Gly Thr Thr Gly Ala Tyr Val 448 ______; _-141- This paper size applies to China National Standard (CNS) A4 specifications ( 210X297 mm) (Please read the notes on the back before filling this page) 509722 A8B8 C8 D8 patent application scope 449 Tyr lie Asp Gin Asp Gly Leu Thr Leu His lie Gin Thr Thr Thr Asn 464 · (Please read the notes on the back before filling this page) 465 Gly Asp Phe lie Asn Arg His Thr Phe Gly Tyr Asn Asp Leu Val Tyr 480 481 Asp Ser Lys Ser Gly Tyr Gly Phe Thr Trp Ser Gly Asn Glu Gly Phe 496 497 Tyr Leu Asp Tyr His Asp Gly Asn Tyr Tyr Thr Phe His Asn Ala lie 512 51 3 lie Asn Tyr Tyr Pro Ser Gly Tyr Gly Gly Gly Ser Val Pro Asn Gly 528 529 Thr Trp Ala Leu Glu Gin Arg lie Asn Glu Gly Trp Ala lie Ala Pro 544 545 Leu Leu Asp Thr Leu His Thr Val Thr Val Lys Gly Ser Tyr lie Ala 560 561 Trp Glu Gly Glu Thr Pro Thr Gly Tyr Asn Leu Tyr lie Pro Asp Gly 576 577 Thr Val Leu Leu Asp Trp Phe Asp Lys lie Asn Phe Ala lie Gly Leu 592 593 Asn Lys Leu Glu Ser Val Phe Thr Ser Pro Asp Trp Pro Thr Leu Thr 608 609 Thr lie Lys Asn Phe Ser Lys lie Ala Asp Asn Arg Lys Phe Tyr Gin 624 625 Glu lie Asn Ala Glu Thr Ala Asp Gly Arg Asn Leu Phe Lys Arg Tyr 640 (CNS) A4 specification (210X297 mm) -142- 509722 A8 B8 C8 D8 VI. Patent application scope 641 Ser Thr Gin Thr Phe Gly Leu Thr Ser Gly Ala Thr Tyr Ser Thr Thr 656 657 Tyr Thr Leu Ser Glu Ala Asp Phe Ser Thr Asp Pro Asp Lys Asn Tyr 672 673 Leu Gin Val Cys Leu Asn Val Val Trp Asp His Tyr Asp Arg Pro Ser 688 689 Gly Lys Lys Gly Ala Tyr Ser Trp Val Ser Lys Trp Phe Asn Val Tyr 704 705 Val Ala Leu Gin Asp Ser Lys Ala Pro Asp Ala lie Pro Arg Leu Val 720 721 Ser Arg Tyr Asp Ser Lys Arg Gly Leu Val Gin Tyr Leu Asp Phe Trp 736 737 Thr Ser Ser Leu Pro Ala Lys Thr Arg Leu Asn Thr Thr Phe Val Arg 752 753 Thr Leu lie Glu Lys Ala Asn Leu Gly Leu Asp Ser Leu Leu Asp Tyr 768 769 Thr Leu Gin Ala Asp Pro Ser Leu Glu Ala Asp Leu Val Thr Asp Gly 784 785 Lys Ser Glu Pro Met Asp Phe Asn Gly Ser Asn Gly Leu Tyr Phe Trp 800 801 Glu Leu Phe Phe His Leu Pro Phe Leu Val Ala Thr Arg Phe Ala Asn 816 817 Glu Gin Gin Phe Ser Pfo Ala Gin Lys Ser Leu His Tyr lie Phe Asp 832 _______; _-143- Applicable to this paper standard National Standards (CNS) A4 size (210 X 297 mm) (Please read the back of the precautions to fill out this page) 509722 A8 B8 C8 D8 Application scope 833 Pro Ala Met Lys Asn Lys Pro His Asn Ala Pro Ala Tyr Trp Asn Val 848 (Please read the notes on the back before filling this page) 849 Arg Pro Leu Val Glu Gly Asn Ser Asp Leu Ser Arg His Leu Asp Asp 864 865 Ser lie Asp Pro Asp Thr Gin Ala Tyr Ala His Pro Val lie Tyr Gin 880 881 Lys Ala Val Phe lie Ala Tyr Val Ser Asn Leu lie Ala Gin Gly Asp 896 897 Met Trp Tyr Arg Gin Leu Thr Arg Asp Gly Leu Thr Gin Ala Arg Val 912 913 Tyr Tyr Asn Leu Ala Ala Glu Leu Leu Gly Pro Arg Pro Asp Val Ser 928 929 Leu Ser Ser lie Trp Thr Pro Gin Thr Leu Asp Thr Leu Ala Ala Gly 944 945 Gin Lys Ala Val Leu Arg Asp Phe Glu His Gin Leu Ala Asn Ser Asp 960 961 Thr Ala Leu Pro Ala Leu Pro Gly Arg Asn Val Ser Tyr Leu Lys Leu 976 977 Ala Asp Asn Gly Tyr Phe Asn Glu Pro Leu Asn Val Leu Met Leu Ser 992 993 His Trp Asp Thr Leu Asp Ala Arg Leu Tyr Asn Leu Arg His Asn Leu 1008 1009 Thr Val Asp Gly Lys Pr-o Leu Ser Leu Pro Leu Tyr Ala Ala Pro Val 1024 This paper size applies to Chinese national standards Standard (CNS) A4 specification (210X297 mm) -144- 509722 A8 B8 C8 D8 VI. Patent application scope 1025 Asp Pro Val Ala Leu Leu Ala Gin Arg Ala Gin Ser Gly Thr Leu Thr 1040 1041 Asn Gly Val Ser Gly Ala Met Leu Thr Val Pro Pro Tyr Arg Phe Ser 1056 1057 Ala Met Leu Pro Arg Ala Tyr Ser Ala Val Gly Thr Leu Thr Ser Phe 1072 1073 Gly Gin Asn Leu Leu Ser Leu Leu Glu Arg Ser Glu A'rg Ala Cys Gin 1088 1089 Glu Glu Leu Ala Gin Gin Gin Leu Leu Asp Met Ser Ser Tyr Ala lie 1104 1105 Thr Leu Gin Gin Gin Gin Ala Leu Asp Gly Leu Ala Ala Asp Arg Leu Ala 1120 1121 Leu Leu Ala Ser Gin Ala Thr Ala Gin Gin Arg His Asp His Tyr Tyr 1136 1137 Thr Leu Tyr Gin Asn Asn lie Ser Ser Ala Glu Gin Leu Val Met Asp 1152 1153 Thr Gin Thr Ser Ala Gin Ser Leu lie Ser Ser Thr Gly Val Gin 1168 1169 Thr Ala Ser Gly Ala Leu Lys Val lie Pro Asn lie Phe Gly Leu Ala 1184 1185 Asp Gly Gly Ser Arg Tyr Glu Gly Val Thr Glu Ala lie Ala lie Gly 1200 1201 Leu Met Ala Ala Gly Gl-n Ala Thr Ser Val Val Ala Glu Arg Leu Ala 1216 paper ruler Degree applies to Chinese National Standard (CNS) A4 specification (210X297 mm) (Please read the precautions on the back before filling this page) 445- 509722 A8 B8 C8 D8 patent application scope 1217 Thr Thr Glu Asn Tyr Arg Arg Arg Arg Glu Glu Trp Gin lie Gin Tyr 1232 * 1233 Gin Gin Ala Gin Ser Glu Val Asp Ala Leu Gin Lys Gin Leu Asp Ala 1248 1249 Leu Ala Val Arg Glu Lys Ala Ala Gin Thr Ser Leu Gin Gin Ala Lys 1264 1265 Ala Gln Gin Val Gin lie Arg Thr Met Leu Thr Tyr Leu Thr Thr Arg 1280 1281 Phe Thr Gin Ala Thr Leu Tyr Gin Trp Leu Ser Gly Gin Leu Ser Ala 1296 1297 Leu Tyr Tyr Gin Ala Tyr Asp Ala Val Val Ala Leu Cys Leu Ser Ala 1312 1313 Gin Ala Cys Trp Gin Tyr Glu Leu Gly Asp Tyr Ala Thr Thr Phe lie 1328 1329 Gin Thr Gly Thr Trp Asn Asp His Tyr Arg Gly Leu Gin Val Gly Glu 1344 1345 Thr Leu Gin Leu Asn Leu His Gin Met Glu Ala Ala Tyr Leu Val Arg 1360 1361 His Glu Arg Arg Leu Asn Val lie Arg Thr Val Ser Leu Lys Ser Leu 1376 1377 Leu Gly Asp Asp Gly Phe Gly Lys Leu Lys Thr Glu Gly Lys Val Asp 1392 1 393 Phe Pro Leu Ser Glu Lys Leu Phe Asp Asn Asp Tyr Pro Gly His Tyr 1408 -146- This paper size applies to China National Standard (CNS) A4 regulations (210X297 mm) .......... attack ........, ΤΓ .... .............. Line · (Please read the notes on the back before filling out this page) 509722 A8 B8 C8 D8 VI. Application scope 1409 Leu Arg Gin ile Lys Thr Val Ser Val Thr Leu Pro Thr Leu Val Gly 1424 * 1425 Pro Tyr Gin Asn Val Lys Ala Thr Leu Thr Gin Thr Ser Ser Ser lie 1440 1441 Leu Leu Ala Ala Asp Ile Asn Gly Val Lys Arg Leu Asn Asp Pro Thr 1456 1457 Gly Lys Glu Gly Asp Ala Thr His Ile Val Thr Asn Leu Arg Ala Ser 1472 1473 Gin Gin Val Ala Leu Ser Ser Gly Ile Asn Asp Ala Gly Ser Phe Glu 1488 1489 Leu Arg Leu Glu Asp Glu Arg Tyr Leu Ser Phe Glu Gly Thr Gly Ala 1504 (Please (Please read the notes on the back before filling this page) 丨 1505 Val Ser Lys Trp Thr Leu Asn Phe Pro Arg Ser Val Asp Glu His Ile 1520 1521 Asp Asp Lys Thr Leu Lys Ala Asp Glu Met Gin Ala Ala Leu Leu Ala 1536 1 537 Asn Met Asp Asp Val Leu Val Gin Val His Tyr Thr Ala Cys Asp Gly 1552 1553 Gly Ala Ser Phe Ala Asn Gin Val Lys Lys Thr Leu Ser 1565 Sequence Identification Editor No. 61: 1 Met Ser Pro Ser Glu Thr Thr Leu Tyr Thr Gin Thr Pro Thr Val Ser 16 17 Val Leu Asp Asn Arg Gly Leu Ser Ile Arg Asp Ile Gly Phe His Arg 32 This paper is sized to the Chinese National Standard (CNS) A4 specification (210X297 mm) -147- 509722 A8 B8 C8 D8 VI. Patent application scope 33 lie Val lie Gly Gly Asp Thr Asp Thr Arg Val Thr Arg His Gin Tyr 48 49 Asp Ala Arg Gly His Leu Asn Tyr Ser lie Asp Pro Arg Leu Tyr Asp 64 65 Ala Lys Gin Ala Asp Asn Ser Val Lys Pro Asn Phe Val Trp Gin His 80 81 Asp Leu Ala Gly His Ala Leu Arg Thr Glu Ser Val Asp Ala Gly Arg 96 97 Thr Val Ala Leu Asn Asp lie Glu Gly Arg Ser Val Met Thr Met Asn 112 113 Ala Thr Gly Val Arg Gin Thr Arg Arg Tyr Glu Gly Asn Thr Leu Pro 128 129 Gly Arg Leu Leu Ser Val Ser Glu Gin Val Phe Asn Gin Glu Ser Ala 144 145 Lys Val Thr Glu Arg Phe lie Trp Ala Gly Asn Thr Thr Ser Glu Lys 160 (Please read the notes on the back before filling in this page)% 161 Glu Tyr Asn Leu Ser Gly Leu Cys lie Arg His Tyr Asp Thr Ala Gly 176 177 Val Thr Arg L eu Met Ser Gin Ser Leu Ala Gly Ala Met Leu Ser Gin 192 193 Ser His Gin Leu Leu Ala Glu Gly Gin Glu Ala Asn Trp Ser Gly Asp 208 209 Asp Glu Thr Val Trp Gin Gly Met Leu Ala Ser Glu Val Tyr Thr Thr 224 This paper size is in accordance with Chinese National Standard (CNS) A4 (210 X 297 mm) -148- 509722 A8 B8 C8 D8 6. Application scope of patent 225 Gin Ser Thr Thr Asn Ala lie Gly Ala Leu Leu Thr Gin Thr Asp Ala 240 241 Lys Gly Asn lie Gin Arg Leu Ala Tyr Asp lie Ala Gly Gin Leu Lys 256 257 Gly Ser Trp Leu Thr Val Lys Gly Gin Ser Glu Gin Val lie Val Lys 272 273 Ser Leu Ser Trp Ser Ala Ala Gly His Lys Leu Arg Glu Glu His Gly 288 289 Asn Gly Val Val Thr Glu Tyr Ser Tyr Glu Pro Glu Thr Gin Arg Leu 304 305 lie Gly lie Thr Thr Arg Arg Ala Glu Gly Ser Gin Ser Gly Ala Arg 320 321 Val Leu Gin Asp Leu Arg Tyr Lys Tyr Asp Pro Val Gly Asn Val lie 336 337 Ser lie His Asn Asp Ala Glu Ala Thr Arg Phe Trp Arg Asn Gin Lys 352 353 Val Glu Pro Glu Asn Arg Tyr Val Tyr Asp Ser Leu Tyr Gin Leu Met 368 369 Ser Ala Thr G ly Arg Glu Met Ala Asn lie Gly Gin Gin Ser Asn Gin 384 385 Leu Pro Ser Pro Val lie Pro Val Pro Thr Asp Asp Ser Thr Tyr Thr 400 401 Asn Tyr Leu Arg Thr Tyr Thr Tyr Asp Arg Gly Gly Asn Leu Val Gin 416 This paper size applies to China National Standard (CNS) A4 (210 X 297 mm) (Please read the precautions on the back before filling this page) -149- 509722 A8 B8 C8 D8 VI. Patent Application Range 417 lie Arg His Ser Ser Pro Ala Thr Gin Asn Ser Tyr Thr Thr Asp lie 432 (Please read the precautions on the back before filling this page) 433 Thr Val Ser Ser Arg Ser Asn Arg Ala Val Leu Ser Thr Leu Thr Thr 448 449 Asp Pro Thr Arg Val Asp Ala Leu Phe Asp Ser Gly Gly His Gin Lys 464 465 Met Leu lie Pro Gly Gin Asn Leu Asp Trp Asn lie Arg Gly Glu Leu 480 481 Gin Arg Val Thr Pro Val Ser Arg Glu Asn Ser Ser Asp Ser Glu Trp 496 497 Tyr Arg Tyr Ser Ser Asp Gly Met Arg Leu Leu Lys Val Ser Glu Gin 512 513 Gin Thr Gly Asn Ser Thr Gin Val Gin Arg Val Thr Tyr Leu Pro Gly 528 529 Leu Glu Leu Arg Thr Thr Gly Val Ala Asp Lys Thr Thr Glu Asp Leu 544 545 Gin Val lie Thr Val Gly Glu Ala Gly Arg Ala Gin Val Arg Val Leu 560 561 His Trp Glu Ser Gly Lys Pro Thr Asp lie Asp Asn Asn Gin Val Arg 576 577 Tyr Ser Tyr Asp Asn Leu Leu Gly Ser Ser Gin Leu Glu Leu Asp Ser 592 593 Glu Gly Gin lie Leu Ser Gin Glu Glu Tyr Tyr Pro Tyr Gly Gly Thr 608 National Standard (CNS) A4 specification (210X297 mm) -150- 509722 A8 B8 C8 D8 ^ _____ 6. Application for patent scope 609 Ala lie Trp Ala Ala Arg Asn Gla Thr Glu Ala Ser Tyr Lys Phe lie 624 · 625 Arg Tyr Ser Gly Lys Glu Arg Asp Ala Thr Gly Leu Tyr Tyr Tyr Gly 640 641 Tyr Arg Tyr Tyr Gin Pro Trp Val Gly Arg Trp Leu Ser Ala Asp Pro 656 657 Ala Gly Thr Val Asp Gly Leu Asn Leu Tyr Arg Met Val Arg Asn Asn 672 673 Pro lie Thr Leu Thr Asp His Asp Gly Leu Ala Pro Ser Pro Asn Arg 688 689 Asn Arg Asn Thr Phe Trp Phe Ala Ser Phe Leu Phe Arg Lys Pro Asp 704 705 Glu Gly Met Ser Ala Ser Met Arg Arg Gly Gin Lys lie Gly Arg Ala 720 721 lie Ala Gly Gly ly Ala lie Gly Gly Leu Ala Ala Thr lie Ala Ala 736 737 Thr Ala Gly Ala Ala lie Pro Vai lie Leu Gly Val Ala Ala Val Gly 752 753 Ala Gly lie Giy Ala Leu Met Giy Tyr Asn Val Gly Ser Leu Leu Glu 768 769 Lys Gly Gly Ala Leu Leu Ala Arg Leu Val Gin Gly Lys Ser Thr Leu 784 785 Val Gin Ser Ala Ala Gly Ala Ala Aia Gly Ala Ser Ser Ala Ala Ala 800 _____- _ _-151- This paper size applies to China National Standard (CNS) A4 (210 X 297 mm) (Please read the precautions on the back before filling out this page)-, blade 丨, line 丨 509722 A8 B8 C8 D8 Patent Application Range 801 Tyr Gly Ala Arg Ala Gin Gly Val Gly Val Ala Ser Ala Ala Gly Ala 816 · (Please read the notes on the back before filling out this page) 817 Val Thr Gly Ala Val Gly Ser Trp lie Asn Asn Ala Asp Arg Gly lie 832 833 Gly Gly Ala lie Gly Ala Gly Ser Ala Val Gly Thr lie Asp Thr Met 848 849 Leu Gly Thr Ala Ser Thr Leu Thr His Glu Val Gly Ala Ala Ala Gly 864 865 Gly Ala Ala Gla Gly Gly Met lie Thr Gly Thr Gin Gly Gly Ser Thr Arg Ala 880 881 Gly lie His Ala Gly lie Gly Thr Tyr Tyr Gly Ser Trp lie Gly Phe 896 897 Gly Leu Asp Val Ala Ser Asn Pro Ala Gly His Leu Ala Asn Tyr Ala 912 913 Val Gly Tyr Ala Ala Gly Leu Gly Ala Glu Met Ala Val Asn Arg lie 928 929 Met Gly Gly Gly Gly Phe Leu Ser Arg Leu Leu Gly Arg Val Val Ser Pro 944 945 Tyr Ala Ala Gly Leu Ala Arg Gin Leu Val His Phe Ser Val Ala Arg 960 961 Pro Val Phe Glu Pro lie Phe Ser Val Leu Gly Gly Leu Val Gly Gly 976 977 lie Gly Thr Gly Leu His Arg Val Met Gly Arg Glu Ser Trp lie Ser 992 This paper size applies to China National Standard (CNS) A4 (210 X 297 mm) -152- 509722 A8 B8 C8 _______D8 VI. Application scope 993 Arg Ala Leu Ser Ala Ala Gly Ser Gly lie Asp His Val Ala Gly Met 1008- 1009 lie Gly Asn Gin lie Arg Gly Arg Val Leu Thr Thr Thr Gly He Ala 1024 1025 Asn Ala lie Asp Tyr Gly Thr Ser Ala Val Gly Ala Ala Arg Arg Val 1040 1041 Phe Ser Leu 1043 2 'The method of claim 1 in which the toxin is sprayed on the plant. 3. The method of claim 1 in which the toxin is applied to the soil in contact with the plant. 4. The method according to item 1 of the patent application, wherein the toxin has an amino acid sequence of sequence identification number 12 (as listed in item 1 of the patent application). 5. · A purified protein, which is the amino acid sequence of sequence identification number 47 (as listed in item 1 of the patent application scope). 6. · A kind of insect attractant: It comprises a bacillus toxin having an oral insecticidal activity as an active component, and a conventional bait matrix, wherein the toxin comprises a polypeptide selected from the group consisting of N-terminal amino acid sequence in the formed group: sequence identification number No. 1, sequence identification number No. 2, sequence identification number No. 3, sequence identification number No. 4, sequence identification number No. 5, sequence Identification No. 6, Sequence Identification No. 7, Sequence Identification No. 8, Sequence Identification No. 9, Sequence Identification No. 10, Sequence Identification -153- (Please read the precautions on the back before filling (This page) The paper size is in accordance with the Chinese National Standard (CNS) A4 (210 X 297 mm) 509722 A8 B8 C8 D8. The scope of patent application f ID No. 13, Serial ID No. 14 and Serial ID No. 15 , Sequence identification number 16th, sequence identification number 17th, sequence identification number 18th, sequence identification number 19th, sequence identification number 20th, sequence identification number 21th, sequence identification No. 22, sequence identification number 23, sequence identification number 24, sequence identification number 37, sequence identification number 38, sequence identification number 39, sequence identification number 40, sequence identification number No. 41, sequence identification number 42, sequence identification number 43, sequence identification number 62, sequence identification number 72, sequence identification number 73, sequence identification number 74, sequence identification number 75, sequence identification number 76, sequence identification number 77, sequence identification number 78, sequence identification number 79, sequence identification number 80, sequence identification number 81, sequence identification number 82 No. 83, Sequence Identification No. 83, Sequence Identification No. 84, Sequence Identification No. 85, Sequence Identification No. 86, Sequence Identification No. 87, and Sequence Identification No. 88 It is shown in item 1 of the patent scope; or wherein the toxin has an amino acid sequence selected from the group consisting of sequence identification number 12 and sequence identification number 26, sequence identification number 28, sequence identification number 30, sequence identification number 32, sequence identification number 34, sequence identification number 35, sequence identification number 47, and sequence identification number 49 No. 51, Sequence Identification No. 51, Sequence Identification No. 53, Sequence Identification No. 55, Sequence Identification No. 57, Sequence -154- (Please read the precautions on the back before filling this page). : Line-This paper size is in accordance with Chinese National Standard (CNS) A4 specification (210X297 mm) 509722 A8 B8 C8 D8 VI. Patent Application Scope Identification Number No. 59 and Sequence Identification Number No. 61. These sequences are subject to application It is shown in item 1 of the patent scope. -155- (Please read the notes on the back before filling out this page) Peak This paper size is applicable to China National Standard (CNS) A4 (210 X 297 mm)