SU1698288A1 - Strain of virus influenza a/leningrad/325/88 for preparation of influenzal diagnosticum - Google Patents

Abstract

This invention relates to virology. The aim of the invention is an influenza A (H1N1) virus strain having high diagnostic activity. For this, strain A / Leningrad / 325/88 (NSh1) is isolated. This strain is characterized by a wide antigenic spectrum and high avidity for antigens. Fro application allowed to increase the frequency of detected seroconversions, which in turn increased the accuracy of diagnosis of influenza infection. 3 tab. SO with

Description

This invention relates to virology. The aim of the invention is an influenza A (H1N1) virus strain having high diagnostic activity.

Morphological signs.

Polymorphic structure: typical of influenza viruses.

Physical properties.

Sedimentation coefficient 750S, buoy density 1.21 g / cm3.

Reproductive activity.

The titer of hemagglutinins - 1: 128-1: 256 in 0.2 ml with 1% chicken erythrocytes, infectious titer - 7.5 Ig EIDbO / 0.2 ml.

The stability of the biological properties of strain A / Leningrad / 325/88 (H1N1) was proved with 5-fold passaging.

Example. An analysis of the use of the strain showed the following. According to the results of cross-rtga with rat monospecific sera, the strain was characterized by a broad antigenic spectrum with respect to antibodies to modern viruses (Table 1).

In addition, the strain of influenza A / Leningrad / 325/88 virus retained a wide range of antigenic activity and avidity for antibodies: B (Table 2) with cross RTGA, it reacts with antiserum to virus A / Prague / 1/84

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Yu

00 Y 00 00

up to 1/4 of the titer, with antiserum to the virus A / Teivan / 1/86 - up to full titer and with antiserum to the virus A / Murmansk / 890/88 - up to a titer twice as high as the titer of this strain with homologous antiserum,

The final analysis of the diagnostic activity of influenza A / Leningrad / 325/88 viruses and the prototype A / Teivan / 1/86 virus was carried out on the material examined in HGV sera of children and adults who had had ARI and vaccinated IVI using diagnostic prepared from these strains

It turned out that the diagnostic activity of s RTGA in the proposed strain is the highest. The use of A / Leningrad / 325/88 virus in comparison with A / Taiwan strain / 1/86 increased the incidence of seroconversion by 3.6%. 5.8%. At the same time, in children, the frequency of diagnosis with the use of the proposed strain increased almost 2 times (12.5 and 6.7%, respectively). In children vaccinated with IHE, the percentage of seroconversions detected increased by 22.2% (70.3% and 48.1%, respectively)

(Table 3).

The geometric mean antibody titers in the sera of the influenza-infected and vaccinated were higher with the use of strain A / Leningrad / 325/88

(H1N1) in comparison with the strain A / Taiwan / 1/86 (H1N1), which is close in antigenic structure, indicating a higher avidity of the proposed strain. The use of strain A / Leningrad / 325/88 (H1N1) has significantly increased the efficiency of diagnosis of modern influenza caused by viruses with antigenic structure A (H1N1).

Claims (1)

Claims of the Invention: Virus influenza A / Leningrad / 325/88 STB No. 2222 for the preparation of influenza diagnosticum The latitude of the antigenic spectrum of influenza A (HINI) viruses 1986-1989, when tested in rtga with monospecific hyperimmune rats sera Note L - antibody titer less than 1 20 Table 1 The results of cross-RTGA viruses of influenza A (H1N1) with hyperimmune rat antisera to the corresponding viruses Table 3 Diagnostic activity of influenza A / Leningrad / 325/88 (H1N1) virus strain table 2