SU1428809A1 - Method of purifying water and soil from oil pollution - Google Patents

Abstract

The invention relates to methods for purifying WATER and soil from oil and soil pollution and can be used to clean contaminated soils 5 of fresh, sea water and industrial waste in various natural and climatic zones. The aim of the invention is to increase the degree of purification from oil pollution. A mixture of the bacterial culture of the natural strain Pseudomonas putida-36 and the mineral fertilizer of the nitroammophosca is used for purification. To obtain the biomass of cells of the Pseudomonas putida-36 strain, the culture is grown in liquid or solid nutrient medium, including nitrogen, phosphorus and potassium sources, in the presence of hydrocarbons under aerobic conditions at a temperature of 30 ° C. The culture of microorganisms is mixed with mineral fertilizer and water from the calculation of - the cells of the indicated strain are not lower than 10 per 1 ml5 and the mineral fertilizer is not lower than 70 mg per 1 l of water. The resulting mixture is uniformly applied to the contaminated surface at a rate of 0.5 - 1.0 l / m, 1 hp, f-ly, 1 tab. S

Description

The invention relates to methods for cleaning water and soil from petroleum of soil contaminants from Pompon; i) microbiological methods.

The purpose of the invention is to increase the degree of purification from oil pollution

The use of natural raTaf wa Pseudomonas putida-36 in combination with the mineral fertilizer Nitroam ofos, which is a source of nitrogen (N), phosphorus () and potassium (), taken in a ratio; 1 ° 1 makes it possible to optimize the conditions of the existence of the bacterium}, and traces a gel, and to increase the efficiency of biodegrading oil and can provide lOOJ -nyic purification from oil and oil products with. The initial content of the PBeudomonas putid8-36 strain should not be lower than 10 living cells in I ml. Such its content ensures sufficient growth and development of cells. An increase in the initial content of biocomponent cells of more than 10 cells / ml makes no sense

as the growth and reproduction of the biocomponent cells increases in the presence of hydrocarbon oil contaminants due to the natural reproduction of Ti-cells:) Kj, starting from a density of 10 ttn / t-vi. Keeping the mixture of mineral matter is fertilized: not less than 70 mg / l will be sufficient , col provision: the growth of the biocomponent with the specified initial cell density. The flow rate of the composition, within the limits of OjS-lsO l per 1 to the area of contamination, regulates the amount of permissible bacterial preparation a. and mineral fertilizer per unit area in a dependency: - bridges on the degree of its loading. The complex mineral fertilizer (.the nitroammofosc complex nitrogen - but phosphorus-potassium fertilizer - contains N, P, Cys, KjO. Taken in the ratio of IPKi consists of the cm mineral:; s. Salts of KNOj, ShtsS15, jPO and ShYKO Releases granules.

The method is carried out in the following way.

To obtain the genus of the biocassa of cells; a And a Pseudomonas putiSa 36 culture is grown on a liquid klak solid nutrient medium; including a source of N5-LM nitrogen of 5 phosphorus to potassium, and the presence of hydrocarbons, the Cult of Shirov ... and is carried out under aerobic conditions under the conditions of the clutch microorganisms grow rapidly and times

.

Q 5 0 5

0 0 j

0

3

} Pyats. If necessary, the obtained biomass of cells can be obtained by the method of freeze-drying or dry air-BOW1H spraying. Mixing is done in a simple mechanical mixing of the cultures; a microorganism with m-geral fertilizer and water at the rate of calculation: the content of living cells of the strain Pseudomonas putida-36 is not lower than 0 cells / ml, and the mineral fertilizer is not 70 mg per liter. The resulting mixture is uniformly applied to the contaminated surface by spraying at the rate of 0, 5-1.0 liters per 1 m.

Example If A plot of soil with an area of 50000 m. Has an oil contamination in a dose of 10 kg of crude oil.

/ J

per 1 m of area The amount of oil in the area is 500 tons. For purification of oil pollution, an aqueous mixture is prepared, including the strain Pseudomonas putida-36 with density CL4 / ML and 70 mg per 1 liter of the complex mineral fertilizer of nitroammofosk. In a volume of 50, the Composition is evenly distributed to the site in a volume of 1 m in I m by spraying AND self-propelled installations. After 2 months after a single treatment, the amount of oil destroyed is 460 tons. “The vegetation is growing & amphibious” in the contaminated area.

PRI mme R 2. A section of a natural freshwater reservoir in the territory of an oil field with a surface contaminated with 23.3 kg / m of oil contains 582.5 tons of crude oil. For the purification of oil pollution 1-W, an aqueous mixture 5 is prepared including a Pseudomonas putida 3IJ strain with a density of 9 x X Q cells, / ml and 70 mg of 1 liter of Kitromask complex mineral fertilizer in a volume of 18.5 m KZ of calculation of 0.75 J of this mixture with 1 m of a polluted surface, Waters mixture is applied evenly by spraying with Bo; 3, After A5 days after treatment, the amount of crude oil is equal to

PRI me R 3, The treatment object is to open ponds of storage for industrial effluents of an oil refinery. Common

reservoir area 800000 m

der; vav eyus v n

de (1zhanie oil "products 1 s (water solution esm co I-3,

der; vav eis in it waters; , j ppm, Sorenic, emulsified and film) is 75 mg / l. To purify oil pollution, a mixture is used that includes the Pseudomonas putida-36 strain with a density of 9–16 cells / ml and 70 mg per 1 liter of the complex mineral fertilizer nitroammophosca in a volume of 400 m of water, which ensures a consumption of 0.5 l per 1 m of pollution . 14 days after a single treatment, the content of oil products in water decreases to 0.5 mg / l and stably maintains at this level for the next 14 days, despite the constant flow of 75 mg / l of oil products into the water body.

Example 4. In a glass tank (length 1.25 m; width 0.8 m and height 0.15 m) 100 l of Kara Sea water and 1720 l of crude oil are added. To clean the oil film, a mixture of 9 x X 10 cells / ml with a nitroammofoski content of 70 mg per liter of solution is used. After 7 days, oil is destroyed by 76.8%,

The proposed method is universal in breadth of use and can be used to clean contaminated

soil, fresh, sea water and industrial | effluents in different climatic zones, including extreme conditions. It is able to purify water with contamination with oil up to 25 kg / m and soil up to 10 kg / m,

Comparative data on the purification of water and soil from oil and oil products are given in the table.

Claims (1)

1. A method of purifying water and soil from oil pollution, including the introduction of a bacterial culture, a natural strain of Pseudomonas putida-36, characterized in that In order to improve the degree of purification, the bacterial culture of the natural strain Pseudomonas putida-36 is introduced in a mixture with a mineral fertilizer with a nitroammophos, 2, the method according to claim 1, characterized by the fact that the bacterial the culture of the natural strain Pseudomonas putida-36 is used in the amount of L not less than 10 cells in 1 ml and a nitroammophoska at least 70 mg / l and the flow rate of the aqueous mixture is 0.5-1.0 l per I m, Vrda with 1% Pseudomonas Netezag-putida-36 96-120 h knowledge Nitroammofosk A mixture of Pseudomonas putida-36 and nitroammophoska Conditions of experience Viocomponents Pseudomonae putida-36 Average 71.60 6.66 Nitroammofosk 2 3 Average A mixture of Pseudomonas put: lda-36 and nitroammophoska Table continuation 7.0 6.9 6.1 6.66 75.1 7A, 9 79.2 76.40 7.9 8.0 8.5 8.13 7.7 8.2 7.73 6.9 6.2 7.0 6.70 99.8  00.0 99, 9 99.90 7.7 7.5 8.0 7.73