SU1347013A1 - Method of determining resistivity of erythrocytes - Google Patents

Abstract

This invention relates to hematology. The purpose of the invention is to increase the sensitivity of the method and its acceleration. Heparinized blood flow is placed in the measuring cell. Gradually increasing the intensity of ultrasound, irradiate the sample under study and control its electrical conductivity. At the time of the beginning of the increase in electrical conductivity, the increase in the intensity of ultrasound is stopped and its measurement is carried out. The measured intensity is parameter-. rum assessments of erythrocyte resistance. i (L: (00

Description

The invention relates to medicine, namely to hematological studies for the purpose of diagnosing diseases of the blood system in toxicosis, bronchopneumonia, and liver cirrhosis.

The purpose of the invention is to increase the sensitivity of the method and its acceleration due to a monotonous increase in the intensity of ultrasound while monitoring the electrical conductivity of the sample under study.

The method is carried out as follows.

Heparinized (or citrate) 5 YuOO Hz. A measuring cell from the source of blood from any vascular zone is placed in a measuring cell containing a small amount of saline solution in order to reduce the minimum required volume of the test sample. Then, having combined the ultrasonic emitter with the surface of the sample under study, it is irradiated with it, gradually increasing the intensity of ultrasound, and at the same time controlling its electrical conductivity. At the time of the beginning of the increase in conductivity, a further increase in the intensity of ultrasound is stopped and its measurement is carried out. The measured intensity, as minimally needed to initiate the hemolysis process, is a parameter for evaluating the resistance of red blood cells.

The device contains a measuring cell, on the opposite walls of which electrodes of an electrical conductivity meter are placed, an ultrasonic device with an external emitter and an ultrasound intensity meter.

To determine the resistance of erythrocytes, the test blood in an amount of 0.1 MP is placed in a measuring cell containing 1 ml of saline. Thereafter . : An ultrasonic emitter is inserted into the cell until it contacts the surface of the test sample and is irradiated with it, gradually increasing the ultrasound intensity using an ultrasound device, starting with a level of 0.01 W / cm. At the same time, the conductivity meter monitors the conductivity of the sample under test with a conductivity meter. At the moment of its beginning; The magnifications stop further increasing the ultrasound intensity, which is measured with an ultrasound intensity meter. In time

Neither TQ ultrasound intensity J, starting with a level of 0.01 W / cm, smoothly increases until time T, when an increase in the electrical conductivity of the EM is recorded. Then, the intensity value J, which serves as an indicator characterizing the resistance of red blood cells, is measured.

When implementing the method, a universal meter L, C, R E7-11 is used as a conductivity meter in the resistance measurement mode at an alternating current frequency

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pressed from plexiglass, electrodes from a platinum wire with a diameter of 1 mm. As an ultrasound device, a commercially available Ultrasound Therapy Device T5 with a remote emitter with an active area of 1 cm and a slight change, consisting in replacing the stepped ultrasound intensity control with a smooth one, is used. This modernization has been reduced to replacing the R27 - R36 resistance device in the circuit diagram with one variable resistance of 100 kΩ and installing it on the external panel of the device. The ultrasound intensity is estimated by measuring the voltage in the feedback circuit of a high-frequency ultrasound-ultrasonic device generator T5 proportional to the voltage applied to the plates of the piezoelectric transducer using a B7-27A voltmeter. With the introduction of ultrasonic vibrations into a blood sample using the Ultrasound-T5 apparatus, in which a piezoelectric transducer from barium titanate is used, the indicated constants have the following meanings:

4, m / s; 1 „16.7 N / V; f 880 kHz; , 13403 kg / m, 1500 m / s.

With

The calibration of the device as a whole at the lower limit of the intensity of the ultra-sound (0.01 W / cm) is carried out by the thermal method.

Instead of the ultrasound-T5 ultrasonic device Ultrasound-T5 used in the implementation of the method of the meter, the B7-27A voltmeter can be used with any commercially available devices of similar purpose with similar technical characteristics.

Tests and experiments carried out on normal and abnormal blood, as well as in the study of the effect of membranotropic drugs, showed that the use of the proposed method allows to reduce by more than 10 times the time and 5 times the sample volume required for the analysis of one sample, and also to increase sensitivity and accuracy as a result of exclusion of a dilution apertures from the procedure and the need to use hematocrit as an auxiliary parameter.

Test 1. A blood sample taken from a purebred rat in an amount of 0.1 ml was placed in a measuring cell containing 1 ml of saline and electrodes connected to a universal E7-11 meter included in the AC resistance measurement mode at 1000 Hz. Then, using the appropriate adjustment knobs of the meter, placed on the front panel, the arrow of the galvanometer is placed at the mark O. After that, the external radiator of the ultrasonic device is inserted into the cell before the radiating surface comes in contact with the liquid surface. The potentiometer knob, which is included in the ultrasonic device instead of the resistance line R 26 - R 37, to the extreme left position. Turn on the Network button of the ultrasonic device and begin to gently rotate the potentiometer knob clockwise. At the same time, the position of the arrow of the galvanometer meter E7-11 is monitored. At the moment when the arrow leaves the scale zero point, the further rotation of the potentiometer knob stops, i.e. increase the intensity of ultrasound, and take a voltmeter reading. Blood samples taken from 15 mongrel rats whose average weight was 180

Compiled M. Sherstnev Editor A. Kozoriz Tehred A. Kravchuk Proofreader M. Demchik

Order 5116/43 Edition 775 Subscription

VNIIPI USSR State Committee

on inventions and discoveries 113035, Moykva, Zh-35, Raushsk nab., 4/5

Production and printing company, Uzhgorod, st. Project, 4

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200 g, they are 141i5 V. The corresponding intensities of ultrasound were 0.08t5-10 3 W / cm.

Rule 2. In a blood sample, taken from the animal used in Test 1, in an amount of 0.2 ml, 10 µl of trifluoroperazine (concentration 10 mol / l) was injected. After mixing, O, 1 ml of the test mass was removed and placed in a measuring cell containing 1 ml of saline. Further analysis is carried out by experiment 1.

The intensity of the ultrasound injected into the sample under study, necessary for the initiation of hemolysis, was 0.04i i7 W / cm2 in this experiment.

Thus, the introduction into the blood of n trifluoroperazine (concentration

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10 mol / l) in an appropriate proportion causes a decrease in resistance of red blood cells.

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Experiments conducted on the same blood samples in a known manner showed that the hemoglobin yield in the analysis of whole blood is 15t1.5 g%, and in the analysis of samples processed with trifluoroperazine by the proposed method, 16t1f%, which confirms the high sensitivity of the latter.

Claims (1)

Invention Formula The method for determining the resistance of erythrocytes by their ultrasound of increasing intensity, characterized in that, in order to increase the sensitivity of the method and its acceleration, the destruction is carried out with a monotonic law of increasing the intensity of ultrasound while monitoring the conductivity of the sample under study and increasing the conductivity relative to the initial level, the resistance is determined red blood cells. 0 five 0 five