SU1345093A1 - Device for metering and changing over microchannels - Google Patents

Abstract

The invention relates to the field of analytical instrumentation, in particular to the instrumentation of the analysis of complex mixtures by liquid chromatography methods, namely, devices for dosing and switching in devices for microanalysis in microcolumn chromatographs or microsequencing agents, and is widely used in biotechnology and pharmaceutical industry. The aim of the invention is to improve the quality of the sample by reducing the dilution, loss and contamination of the microprobes when they are dosed, while maintaining the possibility of production of the device. In a device for metering and switching microchannels consisting of a housing, in the inner cavity of which there is a membrane, filled out of polymer and overlapping the inlet or outlet openings of the underwater channels, against which movable rods are installed in the corresponding recesses of the housing, and there are microchannels submembrane space located along this ring, and the ends of the movable rods, having the form of segments, form a second ring that overlaps the ring of holes, with the center m The diaphragm is rigidly connected to an additional stem, opposite which an additional recess is formed, forming the dosing chamber. 2 Il. "(L with 4 sd about with with

Description

one

The invention relates to analytical instrumentation, in particular to the instrumentation of the analysis of complex mixtures by liquid chromatography methods, and more specifically to devices for metering and switching in devices for microanalysis in microcolumn chromatographs or microsequencing machines, and will find wide application in medical technology, as well as biotechnology and

pharmaceutical industry.

The aim of the invention is to improve the quality of the sample by reducing the erosion, loss and contamination of the microprobe dosing while maintaining the possibility of washing the device.

Fig, 1 shows the proposed device, the cut; in fig. 2 - ring holder and ring, formed by the ends of the liTOKOB, top view,

The housing 1 of the metering and switching device in the internal cavity 2 contains a membrane 3 made of polymer and overlapping the inlet or outlet apertures of the underwater and exhaust channels 4 opposite to which movable rods 5 with ends 6 are placed in the device case in the corresponding recesses 7. The center of the membrane is rigidly connected with an additional movable rod 8, opposite to which a recess in the form of a metering chamber 9 with a central channel 10 is formed in the internal cavity of the device casing,

The ends of the movable rods are in the form of segments that form a ring And ,, overlapping ring holder 12 ,. passing through the centers of the holes of the underwater channels.

The device works as follows.

The extreme upper position of the movable rod 8 is determined, which determines the volume of the dosing capacity in the internal cavity 2 of the dosing device housing 1 bounded by the membrane 3 and the recess 9, while the central channel 10 is blocked, the desired moving rod 5 is recessed in the housing 7. In this case, the end of the stem 6 opens one of the outlet openings 4, and the liquid enters the dosing tank 2 through the opening channel. The raised stem is lowered and the movable

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the rod over the channel where the selected portion of the liquid is to be injected. To push the liquid over the dosing tank (the moving rod 8 is lowered and the membrane 3 is pressed against the surface of the recess 9). In the case of simple switching of the channels, the rod 8 is lowered a little above the surface of the recess 9, so that the volume of the submembrane space is commensurate with the volume of the section of the underwater channel 4 with a length equal to the diameter of the holder 12,

After long-term operation, in order to avoid accumulation of contaminants, a washing liquid is fed into the submembrane space through the channel 10, and then alternately begin to open each sector of the ring 11 and discharge the washing liquid.

The advantage of the proposed device is the ability to feed into the dosing tank at the same time

several solutions and, on the contrary, the supply of the solution from the dosing tank to several channels at once.

The technique of microanalysis has a whole range of specific features, the main of which should be considered to be the reduction to the technically achievable minimum lengths of connecting lines and all auxiliary service volumes involved in the process.

dosing and switching. This plays a particularly significant role in the introduction of trace amounts of reagents with a volume of 0.1-1.0 µl in a microsequencer, where it is necessary to eliminate sample erosion and at the same time preserve the possibility of flushing the dosing volume and connecting lines.

Compared with device pro-. By the way, the proposed device provides the best hydrodynamic conditions for supplying the veins: the transfer from the dosing chamber to the channels, thus reducing the contamination of the device, losses and smearing of the sample. The shape of the ends of the movable rods provides for clamping the membrane around the perimeter around the dosing chamber along a wide ring above the inlet openings circumferentially around the central opening. Due to the circular symmetry of the channel openings and the possibility of creating pulsations of the membrane by the movement of the central movable rod, better conditions for flushing the device are provided.

Claims (1)

Invention Formula e ni A device for metering and switching microchannels, consisting of a housing with channels for supplying and discharging reagents, in the internal cavity of which a membrane is located that overlaps the openings of the channels, and movable rods placed in the slots of the housing coaxially, which are the objective is to improve the quality of the sample by reducing erosion, loss and contamination microprobes when they are dosed while maintaining the possibility of washing the device, the housing is made with a central dosing chamber equipped with an additional movable rod placed in its upper part and rigidly connected to the center of the membrane, the device is equipped with an annular holder, channels for supplying and discharging reagents are placed on the holder, the ends of the movable rod is made in the form of segments, installed without a gap along the side forming with the formation of an annular plate installed under the end holder with the possible overlap of channels.  J g / W 9 2. / i Editor A.Makovska Compiled by L.Nechyporenko Tehred M. Khodanich Proofreader A.T. Order 4912/42 Circulation 776 Subscription VNIIPI USSR State Committee for Inventions and Discoveries 4/5, Moscow, Zh-35, Raushsk nab. 113035 Production and printing company, Uzhgorod, st. Project, 4 FIG. 2