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SG11202107921SA - Efficient product cleavage in template-free enzymatic synthesis of polynucleotides. - Google Patents

Efficient product cleavage in template-free enzymatic synthesis of polynucleotides.

Info

Publication number
SG11202107921SA
SG11202107921SA SG11202107921SA SG11202107921SA SG11202107921SA SG 11202107921S A SG11202107921S A SG 11202107921SA SG 11202107921S A SG11202107921S A SG 11202107921SA SG 11202107921S A SG11202107921S A SG 11202107921SA SG 11202107921S A SG11202107921S A SG 11202107921SA
Authority
SG
Singapore
Prior art keywords
polynucleotides
template
enzymatic synthesis
efficient product
free enzymatic
Prior art date
Application number
SG11202107921SA
Inventor
Sandrine Creton
Original Assignee
Dna Script
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dna Script filed Critical Dna Script
Publication of SG11202107921SA publication Critical patent/SG11202107921SA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase

Landscapes

  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
SG11202107921SA 2019-02-12 2020-02-11 Efficient product cleavage in template-free enzymatic synthesis of polynucleotides. SG11202107921SA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP19305174 2019-02-12
PCT/EP2020/053417 WO2020165137A1 (en) 2019-02-12 2020-02-11 Efficient product cleavage in template-free enzymatic synthesis of polynucleotides.

Publications (1)

Publication Number Publication Date
SG11202107921SA true SG11202107921SA (en) 2021-08-30

Family

ID=65635610

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202107921SA SG11202107921SA (en) 2019-02-12 2020-02-11 Efficient product cleavage in template-free enzymatic synthesis of polynucleotides.

Country Status (10)

Country Link
US (2) US11359221B2 (en)
EP (1) EP3924500A1 (en)
JP (1) JP7538807B2 (en)
KR (1) KR20210125496A (en)
CN (1) CN113423840B (en)
AU (1) AU2020223456A1 (en)
CA (1) CA3129393A1 (en)
IL (1) IL285364B1 (en)
SG (1) SG11202107921SA (en)
WO (1) WO2020165137A1 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113272442B (en) * 2019-01-03 2024-03-15 Dna斯克瑞普特公司 One-pot synthesis of pools of oligonucleotides
JP7538807B2 (en) 2019-02-12 2024-08-22 ディーエヌエー スクリプト Efficient product cleavage in template-free enzymatic polynucleotide synthesis.
GB202013102D0 (en) 2020-08-21 2020-10-07 Nuclera Nucleics Ltd Methods of nucleic acid synthesis
WO2022090057A1 (en) 2020-10-26 2022-05-05 Dna Script Novel variants of endonuclease v and uses thereof
US20240052391A1 (en) * 2020-10-29 2024-02-15 Dna Script Enzymatic Synthesis of Polynucleotide Probes
CN117222744A (en) 2021-04-02 2023-12-12 Dna斯克瑞普特公司 Method and kit for enzymatic synthesis of G4-explosive polynucleotides
US20220389500A1 (en) * 2021-05-26 2022-12-08 Illumina, Inc. Purifying and polymerizing 3'-blocked nucleotides
DE102022114351A1 (en) 2021-06-10 2022-12-15 Dna Script ENZYMATIC SYNTHESIS OF POLYNUCLEOTIDES USING 3'-O-AMINO-2'-DEOXYRIBONUCLEOSIDE TRIPHOSPHATE MONOMERS
WO2024083883A1 (en) 2022-10-19 2024-04-25 Dna Script Methods and products for removal of uracil containing polynucleotides
WO2024121022A1 (en) 2022-12-05 2024-06-13 Dna Script Variants of poly(a) polymerase and uses thereof
WO2024146937A1 (en) 2023-01-06 2024-07-11 Dna Script Methods for obtaining correctly assembled nucleic acids
WO2024153642A1 (en) 2023-01-16 2024-07-25 Dna Script Scarless template-free enzymatic synthesis of polynucleotides
WO2024156896A2 (en) * 2023-01-26 2024-08-02 Dna Script Enzymatic synthesis of polynucleotide probes

Family Cites Families (68)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5367066A (en) 1984-10-16 1994-11-22 Chiron Corporation Oligonucleotides with selectably cleavable and/or abasic sites
US4772691A (en) 1985-06-05 1988-09-20 The Medical College Of Wisconsin, Inc. Chemically cleavable nucleotides
US5262530A (en) 1988-12-21 1993-11-16 Applied Biosystems, Inc. Automated system for polynucleotide synthesis and purification
US5047524A (en) 1988-12-21 1991-09-10 Applied Biosystems, Inc. Automated system for polynucleotide synthesis and purification
WO1991006678A1 (en) 1989-10-26 1991-05-16 Sri International Dna sequencing
US5516664A (en) 1992-12-23 1996-05-14 Hyman; Edward D. Enzymatic synthesis of repeat regions of oligonucleotides
US5436143A (en) 1992-12-23 1995-07-25 Hyman; Edward D. Method for enzymatic synthesis of oligonucleotides
FR2703052B1 (en) 1993-03-26 1995-06-02 Pasteur Institut New method of nucleic acid sequencing.
US5656745A (en) 1993-09-17 1997-08-12 Gilead Sciences, Inc. Nucleotide analogs
AU1436995A (en) 1993-12-30 1995-07-17 Chemgenes Corporation Synthesis of propargyl modified nucleosides and phosphoramidites and their incorporation into defined sequence oligonucleotides
US5872244A (en) 1994-09-02 1999-02-16 Andrew C. Hiatt 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds
US6232465B1 (en) 1994-09-02 2001-05-15 Andrew C. Hiatt Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides
US5808045A (en) 1994-09-02 1998-09-15 Andrew C. Hiatt Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides
US6214987B1 (en) 1994-09-02 2001-04-10 Andrew C. Hiatt Compositions for enzyme catalyzed template-independent formation of phosphodiester bonds using protected nucleotides
US5763594A (en) 1994-09-02 1998-06-09 Andrew C. Hiatt 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds
US5990300A (en) 1994-09-02 1999-11-23 Andrew C. Hiatt Enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides
US5700642A (en) 1995-05-22 1997-12-23 Sri International Oligonucleotide sizing using immobilized cleavable primers
US5917031A (en) 1996-04-19 1999-06-29 Taiko Pharmaceutical Co., Ltd. Method of synthesizing polydeoxyribonucleotides
EP1961826A3 (en) 1999-03-10 2008-09-17 ASM Scientific, Inc. A method for direct nucleic acid sequencing
GB9907813D0 (en) 1999-04-06 1999-06-02 Medical Biosystems Ltd Synthesis
GB0013276D0 (en) 2000-06-01 2000-07-26 Amersham Pharm Biotech Uk Ltd Nucleotide analogues
CN1854309A (en) * 2000-08-23 2006-11-01 宝生物工程株式会社 Method of amplifying nucleic acid
AU2001296645A1 (en) 2000-10-06 2002-04-15 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding dna and rna
US6777189B2 (en) 2001-03-30 2004-08-17 Applera Corporation Nucleic acid analysis using non-templated nucleotide addition
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
DE10215035A1 (en) 2002-04-05 2003-10-23 Roche Diagnostics Gmbh Recombinant terminal deoxynucleotide transferase with improved functionality
WO2004005667A1 (en) 2002-07-08 2004-01-15 Shell Internationale Research Maatschappij B.V. Choke for controlling the flow of drilling mud
CN1681923B (en) * 2002-07-18 2010-06-02 孟山都技术有限公司 Methods for using artificial polynucleotides and compositions thereof to reduce transgene silencing
EP2607369B1 (en) 2002-08-23 2015-09-23 Illumina Cambridge Limited Modified nucleotides for polynucleotide sequencing
US7932025B2 (en) 2002-12-10 2011-04-26 Massachusetts Institute Of Technology Methods for high fidelity production of long nucleic acid molecules with error control
JP2006513705A (en) 2003-02-05 2006-04-27 アマシャム・バイオサイエンス・コーポレイション Nucleic acid growth
US7572581B2 (en) 2003-06-30 2009-08-11 Roche Molecular Systems, Inc. 2′-terminator nucleotide-related methods and systems
US7947817B2 (en) 2003-06-30 2011-05-24 Roche Molecular Systems, Inc. Synthesis and compositions of 2'-terminator nucleotides
US7790374B2 (en) * 2003-10-02 2010-09-07 Basf Aktiengesellschaft Process for sequence saturation mutagenesis (SeSaM)
US7407757B2 (en) 2005-02-10 2008-08-05 Population Genetics Technologies Genetic analysis by sequence-specific sorting
US8212020B2 (en) 2005-03-11 2012-07-03 Steven Albert Benner Reagents for reversibly terminating primer extension
US7544794B1 (en) 2005-03-11 2009-06-09 Steven Albert Benner Method for sequencing DNA and RNA by synthesis
JP4822801B2 (en) * 2005-10-24 2011-11-24 西川ゴム工業株式会社 Mutant endonuclease
JP4918409B2 (en) * 2006-07-26 2012-04-18 西川ゴム工業株式会社 Nucleic acid sequence amplification method
WO2008042067A2 (en) 2006-09-28 2008-04-10 Illumina, Inc. Compositions and methods for nucleotide sequencing
DK2171088T3 (en) 2007-06-19 2016-01-25 Stratos Genomics Inc Nucleic acid sequencing in a high yield by expansion
WO2010030716A1 (en) 2008-09-10 2010-03-18 Igor Kutyavin Detection of nucleic acids by oligonucleotide probes cleaved in presence of endonuclease v
US8034923B1 (en) 2009-03-27 2011-10-11 Steven Albert Benner Reagents for reversibly terminating primer extension
US8674086B2 (en) 2010-06-25 2014-03-18 Intel Corporation Nucleotides and oligonucleotides for nucleic acid sequencing
US9896709B2 (en) 2012-03-13 2018-02-20 Swift Biosciences, Inc. Methods and compositions for size-controlled homopolymer tailing of substrate polynucleotides by a nucleic acid polymerase
WO2013191775A2 (en) 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US9279149B2 (en) 2013-04-02 2016-03-08 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acids
US8808989B1 (en) 2013-04-02 2014-08-19 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acids
EP2876166B1 (en) 2013-11-20 2016-12-14 Roche Diagnostics GmbH New compound for sequencing by synthesis
FR3020071B1 (en) 2014-04-17 2017-12-22 Dna Script PROCESS FOR THE SYNTHESIS OF NUCLEIC ACIDS, IN PARTICULAR LARGE NUCLEIC ACIDS, USE OF THE METHOD AND KIT FOR IMPLEMENTING THE METHOD
FR3025201B1 (en) 2014-09-02 2018-10-12 Dna Script MODIFIED NUCLEOTIDES FOR THE SYNTHESIS OF NUCLEIC ACIDS, A KIT COMPRISING SUCH NUCLEOTIDES AND THEIR USE FOR GENERATING SYNTHETIC NUCLEIC ACID GENES OR SEQUENCES
GB201502152D0 (en) * 2015-02-10 2015-03-25 Nuclera Nucleics Ltd Novel use
GB201503534D0 (en) 2015-03-03 2015-04-15 Nuclera Nucleics Ltd Novel method
GB201512372D0 (en) 2015-07-15 2015-08-19 Nuclera Nucleics Ltd Novel method
FR3052462A1 (en) * 2016-06-14 2017-12-15 Dna Script POLYMERASE DNA VARIANTS OF THE POLX FAMILY
GB2559117B (en) 2017-01-19 2019-11-27 Oxford Nanopore Tech Ltd Double stranded polynucleotide synthesis method, kit and system
GB201701396D0 (en) 2017-01-27 2017-03-15 Nuclera Nucleics Ltd Novel alternating-phase synthesis method
GB201718804D0 (en) 2017-11-14 2017-12-27 Nuclera Nucleics Ltd Novel use
EP3737749A1 (en) 2018-01-08 2020-11-18 DNA Script Variants of terminal deoxynucleotidyl transferase and uses thereof
US10752887B2 (en) 2018-01-08 2020-08-25 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof
CA3106410A1 (en) 2018-07-23 2020-01-30 Dna Script Massively parallel enzymatic synthesis of nucleic acid strands
EP3844167A1 (en) 2018-08-31 2021-07-07 DNA Script Method for preparing 3'-o-amino-2'-deoxyribonucleoside-5'-triphosphate
CN113195720A (en) 2018-10-12 2021-07-30 哈佛大学的校长及成员们 Enzymatic RNA synthesis
JP2022513031A (en) 2018-11-14 2022-02-07 ディーエヌエー スクリプト Terminal deoxynucleotidyl transferase variant and its use
WO2020120442A2 (en) * 2018-12-13 2020-06-18 Dna Script Direct oligonucleotide synthesis on cells and biomolecules
JP7538807B2 (en) 2019-02-12 2024-08-22 ディーエヌエー スクリプト Efficient product cleavage in template-free enzymatic polynucleotide synthesis.
US11312742B2 (en) 2019-02-14 2022-04-26 Dna Script Sas Process for preparing 3'-O-amino-ribonucleotide

Also Published As

Publication number Publication date
US20230105977A1 (en) 2023-04-06
CN113423840A (en) 2021-09-21
CA3129393A1 (en) 2020-08-20
WO2020165137A1 (en) 2020-08-20
CN113423840B (en) 2024-03-08
US11905541B2 (en) 2024-02-20
JP2022520574A (en) 2022-03-31
AU2020223456A1 (en) 2021-08-12
US20210254114A1 (en) 2021-08-19
IL285364A (en) 2021-09-30
US11359221B2 (en) 2022-06-14
KR20210125496A (en) 2021-10-18
EP3924500A1 (en) 2021-12-22
JP7538807B2 (en) 2024-08-22
IL285364B1 (en) 2024-09-01

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