Nothing Special   »   [go: up one dir, main page]

SG11201808740TA - Multiplex nucleic acid assay methods capable of detecting closely related alleles, and reagents therefor - Google Patents

Multiplex nucleic acid assay methods capable of detecting closely related alleles, and reagents therefor

Info

Publication number
SG11201808740TA
SG11201808740TA SG11201808740TA SG11201808740TA SG11201808740TA SG 11201808740T A SG11201808740T A SG 11201808740TA SG 11201808740T A SG11201808740T A SG 11201808740TA SG 11201808740T A SG11201808740T A SG 11201808740TA SG 11201808740T A SG11201808740T A SG 11201808740TA
Authority
SG
Singapore
Prior art keywords
international
nucleic acid
assay methods
pct
street
Prior art date
Application number
SG11201808740TA
Inventor
Salvatore A E Marras
Diana Vargas-Gold
Sanjay Tyagi
Fred Russell Kramer
Original Assignee
Rutgers The State University Of New Jersey
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rutgers The State University Of New Jersey filed Critical Rutgers The State University Of New Jersey
Publication of SG11201808740TA publication Critical patent/SG11201808740TA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -, Organization 111111110111101110101011111010111110111010111111011111111101011111101111011111 International Bureau 0.. .... .. (10) International Publication Number (43) International Publication Date ...... ../ WO 2017/176852 Al 12 October 2017(12.10.2017) WIPO I PCT (51) International Patent Classification: (74) C12Q 1/68 (2006.01) Cl 2P 19/30 (2006.01) Agents: NORTON, Gerard P. et al.; Fox Rothschild LLP, 997 Lenox Drive, Building 3, Lawrenceville, NJ 08648- C12Q 1/08 (2006.01) 2311 (US). (21) International Application Number: (81) PCT/US2017/026088 Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, (22) International Filing Date: 5 April 2017 (05.04.2017) BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, (25) Filing Language: English HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, (26) Publication Language: English MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, (30) Priority Data: 62/319,332 7 April 2016 (07.04.2016) US NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, (71) Applicant: RUTGERS, THE STATE UNIVERSITY OF ZA, ZM, ZW. NEW JERSEY [US/US]; 83 Somerset Street, New Brun- swick, NJ 08901 (US). (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, (72) Inventors: MARRAS, Salvatore A.E.; 104 West Roselle GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, Avenue, Roselle Park, NJ 07204 (US). VARGAS-GOLD, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, Diana; 119 Millburn Avenue, Millburn, NJ 07041 (US). TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, TYAGI, Sanjay; 99 John Street, Apt 2109, New York, NY DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, = 10038 (US). KRAMER, Fred Russell; 561 West 231 LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, Street, Riverdale, NY 10463 (US). = [Continued on next page] (54) Title: MULTIPLEX NUCLEIC ACID ASSAY METHODS CAPABLE OF DETECTING CLOSELY RELATED ALLELES, = = AND REAGENTS THEREFOR (57) : This invention discloses multi-part = FIGURE 1 163 1J7 105 primers for primer-dependent nucleic acid amplification methods. Also disclosed are multiplex assay methods, related reagent kits, and oligonucleotides for such meth- ods. = — = - - _ = kY 106 103 1'1 = 110 = 210 211 IF , = 212- = 105 213 ' 4. 4 104 106 r --2D1 ......,...,107 = 204.,i 1 = 2 205 03 11 7 ei 105 104 106 kin 210 215 GC 211 2 45 11 /6 IN 11 ei O WO 2017/176852 Al MIDEDIM01101DERDEEMOM01011110111111111111101111111011110111111 SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, Published: GW, KM, ML, MR, NE, SN, TD, TG). — with international search report (Art. 21(3))
SG11201808740TA 2016-04-07 2017-04-05 Multiplex nucleic acid assay methods capable of detecting closely related alleles, and reagents therefor SG11201808740TA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662319332P 2016-04-07 2016-04-07
PCT/US2017/026088 WO2017176852A1 (en) 2016-04-07 2017-04-05 Multiplex nucleic acid assay methods capable of detecting closely related alleles, and reagents therefor

Publications (1)

Publication Number Publication Date
SG11201808740TA true SG11201808740TA (en) 2018-11-29

Family

ID=60000654

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201808740TA SG11201808740TA (en) 2016-04-07 2017-04-05 Multiplex nucleic acid assay methods capable of detecting closely related alleles, and reagents therefor

Country Status (9)

Country Link
US (1) US11542547B2 (en)
EP (1) EP3440226B1 (en)
JP (1) JP6933858B2 (en)
CN (1) CN109312398B (en)
AU (1) AU2017246663B2 (en)
CA (1) CA3020165A1 (en)
SG (1) SG11201808740TA (en)
WO (1) WO2017176852A1 (en)
ZA (1) ZA201807405B (en)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10017812B2 (en) 2010-05-18 2018-07-10 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US20140100126A1 (en) 2012-08-17 2014-04-10 Natera, Inc. Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data
US11479812B2 (en) 2015-05-11 2022-10-25 Natera, Inc. Methods and compositions for determining ploidy
WO2019118926A1 (en) 2017-12-14 2019-06-20 Tai Diagnostics, Inc. Assessing graft suitability for transplantation
CN111699269A (en) * 2018-01-12 2020-09-22 纳特拉公司 Novel primer and use thereof
US12024738B2 (en) 2018-04-14 2024-07-02 Natera, Inc. Methods for cancer detection and monitoring
EP3856931B1 (en) * 2018-09-25 2023-10-11 Co-Diagnostics, Inc. Allele-specific design of cooperative primers for improved nucleic acid variant genotyping
CN110964814B (en) * 2018-09-30 2024-05-03 迈克生物股份有限公司 Primers, compositions and methods for nucleic acid sequence variation detection
CN111349691B (en) * 2018-12-21 2023-10-20 迈克生物股份有限公司 Composition, kit and detection method for EGFR gene deletion mutation detection
CN110541033B (en) * 2019-09-27 2023-11-14 迈克生物股份有限公司 Composition for EGFR gene mutation detection and detection method
CN110592215A (en) * 2019-09-27 2019-12-20 迈克生物股份有限公司 Composition for detecting nucleic acid sequence and detection method
EP4038197A4 (en) 2019-10-02 2023-08-23 Rutgers, the State University of New Jersey Assay methods and kits for detecting rare sequence variants
CN112824535B (en) * 2019-11-21 2023-07-04 迈克生物股份有限公司 Primer composition for gene mutation multiplex detection and kit thereof
CN116121358B (en) * 2022-11-11 2024-07-23 重庆浦济生命科技有限公司 Composition for psychotropic drug gene detection, kit and use method

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7198897B2 (en) 2001-12-19 2007-04-03 Brandeis University Late-PCR
US20060141518A1 (en) * 2004-03-24 2006-06-29 Lao Kai Q Detection of gene expression
EP3118333B1 (en) * 2006-12-13 2019-04-03 Luminex Corporation Systems and methods for multiplex analysis of pcr in real time
JP6544861B2 (en) * 2013-02-07 2019-07-17 ラトガース，ザ ステート ユニバーシティ オブ ニュー ジャージー Highly selective nucleic acid amplification primer
EP3044337B1 (en) * 2013-09-13 2020-03-18 Rutgers, the State University of New Jersey Multiplex diagnostic assay for lyme disease and other tick-borne diseases
GB201409786D0 (en) * 2014-06-02 2014-07-16 Primer Design Ltd Nucleic acid amplification system
CA2956174A1 (en) * 2014-07-24 2016-01-28 Brandeis University Linear-expo-linear pcr (lel-pcr)

Also Published As

Publication number Publication date
ZA201807405B (en) 2021-04-28
EP3440226A1 (en) 2019-02-13
CN109312398A (en) 2019-02-05
CA3020165A1 (en) 2017-10-12
EP3440226A4 (en) 2019-11-13
AU2017246663B2 (en) 2022-09-01
US11542547B2 (en) 2023-01-03
CN109312398B (en) 2022-05-31
WO2017176852A1 (en) 2017-10-12
JP2019510501A (en) 2019-04-18
EP3440226B1 (en) 2021-02-17
AU2017246663A1 (en) 2018-11-22
US20190225999A1 (en) 2019-07-25
JP6933858B2 (en) 2021-09-08

Similar Documents

Publication Publication Date Title
SG11201808740TA (en) Multiplex nucleic acid assay methods capable of detecting closely related alleles, and reagents therefor
SG11201810003UA (en) Using programmable dna binding proteins to enhance targeted genome modification
SG11201807068WA (en) Devices and methods for rapid pcr
SG11201810611YA (en) Coumarin compounds and their uses as fluorescent labels
SG11201809913PA (en) Methods for detecting target nucleic acids in a sample
SG11201809522WA (en) Method of nucleic acid sequence determination
SG11201808721YA (en) Cell-free production of ribonucleic acid
SG11201908564RA (en) Devices, processes, and systems for determination of nucleic acid sequence, expression, copy number, or methylation changes using combined nuclease, ligase, polymerase, and sequencing reactions
SG11201805592RA (en) Methods of producing patient-specific anti-cancer therapeutics and methods of treatment therefor
SG11201811740WA (en) Systems and methods for identifying risky driving behavior
SG11201908719QA (en) Biomarkers and car t cell therapies with enhanced efficacy
SG11201908396PA (en) Methods of treating tumor
SG11201908658TA (en) Nucleobase editors comprising nucleic acid programmable dna binding proteins
SG11201900746RA (en) Engineered antibodies and other fc-domain containing molecules with enhanced agonism and effector functions
SG11201806663TA (en) Closed-ended linear duplex dna for non-viral gene transfer
SG11201805835WA (en) Modified oncolytic virus
SG11201806282XA (en) High-throughput identification of patient-specific neoepitopes as therapeutic targets for cancer immunotherapies
SG11201908056QA (en) Anti-par2 antibodies and uses thereof
SG11201810430WA (en) Heater assembly for an aerosol-generating system
SG11201806946VA (en) Epigenomic profiling reveals the somatic promoter landscape of primary gastric adenocarcinoma
SG11201808022WA (en) Selection and cloning of t lymphocytes in a microfluidic device
SG11201910115XA (en) Rnai approach for crop pest protection
SG11201901172QA (en) A method for manufacturing a fuel component
SG11201910195WA (en) Methods for trapping and barcoding discrete biological units in hydrogel
SG11201810914VA (en) Compositions and methods for diagnosing lung cancers using gene expression profiles