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SG11201806557VA - Differentiation induction from human pluripotent stem cells into hypothalamic neurons - Google Patents

Differentiation induction from human pluripotent stem cells into hypothalamic neurons

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Publication number
SG11201806557VA
SG11201806557VA SG11201806557VA SG11201806557VA SG11201806557VA SG 11201806557V A SG11201806557V A SG 11201806557VA SG 11201806557V A SG11201806557V A SG 11201806557VA SG 11201806557V A SG11201806557V A SG 11201806557VA SG 11201806557V A SG11201806557V A SG 11201806557VA
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Singapore
Prior art keywords
pluripotent stem
human pluripotent
stem cells
suspension
substance
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Application number
SG11201806557VA
Inventor
Hidetaka Suga
Koichiro Ogawa
Takatoshi Kasai
Hiroshi Arima
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Univ Nagoya Nat Univ Corp
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Publication date
Application filed by Univ Nagoya Nat Univ Corp filed Critical Univ Nagoya Nat Univ Corp
Publication of SG11201806557VA publication Critical patent/SG11201806557VA/en

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Abstract

DIFFERENTIATION INDUCTION FROM HUMAN PLURIPOTENT STEM CELLS INTO HYPOTHALAMIC NEURONS 5 There is provided a method for efficient differentiation induction from human pluripotent stem cells into hypothalamic neurons. Also, provided is a method for constructing, from human pluripotent stem cells, a cellular structure in which hypothalamic tissue and pituitary tissue are integrated. A cellular structure including hypothalamic tissue is obtained by a method including the steps of: culturing an aggregate of human pluripotent stem 10 cells in suspension in a medium containing a low concentration of a bone morphogenetic protein signal transduction pathway activating substance and a low concentration of a substance acting on the Shh signaling pathway; and further culturing the cell aggregate obtained in the step in suspension in a medium containing a low concentration of a substance acting on the Shh signaling pathway. Also, a cellular structure including hypothalamic tissue 15 and pituitary tissue is obtained by a method including the steps of: culturing an aggregate of human pluripotent stem cells in suspension in a medium containing a bone morphogenetic protein signal transduction pathway activating substance and a substance acting on the Shh signaling pathway; further culturing the cell aggregate formed in the step in suspension in a medium containing a bone morphogenetic protein signal transduction pathway activating 20 substance and a substance acting on the Shh signaling pathway; and culturing the cell aggregate obtained in the step in suspension in a medium suitable for simultaneous induction of pituitary and hypothalamus. [Figure 14] 25
SG11201806557VA 2016-01-22 2017-01-18 Differentiation induction from human pluripotent stem cells into hypothalamic neurons SG11201806557VA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2016010940 2016-01-22
PCT/JP2017/001544 WO2017126551A1 (en) 2016-01-22 2017-01-18 Differentiation induction from human pluripotent stem cells into hypothalamic neurons

Publications (1)

Publication Number Publication Date
SG11201806557VA true SG11201806557VA (en) 2018-08-30

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Country Status (11)

Country Link
US (1) US20190010452A1 (en)
EP (1) EP3406709A4 (en)
JP (2) JP7023496B2 (en)
KR (1) KR20180101467A (en)
CN (1) CN108495929B (en)
AU (1) AU2017209742B2 (en)
CA (1) CA3013553A1 (en)
IL (1) IL260380B2 (en)
MY (1) MY194128A (en)
SG (1) SG11201806557VA (en)
WO (1) WO2017126551A1 (en)

Families Citing this family (7)

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Publication number Priority date Publication date Assignee Title
EP3715450A4 (en) * 2017-11-24 2021-08-25 Sumitomo Chemical Company, Limited METHOD OF PREPARING CELL MASS, INCLUDING HYPOPHYSICAL TISSUE, AND CELL MASS THEREOF
CN115175989A (en) 2019-11-20 2022-10-11 住友制药株式会社 Method for freezing nerve cells
WO2021100830A1 (en) 2019-11-20 2021-05-27 大日本住友製薬株式会社 Method for freezing cell aggregates
CN113046306B (en) * 2021-03-12 2022-10-18 广东东阳光药业有限公司 Culture method of pluripotent stem cells
JPWO2023054395A1 (en) * 2021-09-30 2023-04-06
JPWO2023054396A1 (en) * 2021-09-30 2023-04-06
WO2024248023A1 (en) * 2023-05-29 2024-12-05 京都府公立大学法人 Method for producing suprachiasmatic nucleus organoid

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RU2345133C2 (en) * 2003-03-12 2009-01-27 Релайанс Лайф Сайенсиз Пвт. Лтд. Production of terminal-differentiated dofaminnergic neurones from embrionic human founder cells
WO2013065763A1 (en) * 2011-10-31 2013-05-10 独立行政法人理化学研究所 Method for culturing stem cell
EP2970897B1 (en) 2013-03-14 2019-11-27 The Regents of The University of California In vitro production of medial ganglionic eminence precursor cells
ES2732730T3 (en) * 2013-11-22 2019-11-25 Riken Method to produce telencephalon or progenitor tissue thereof
WO2016013669A1 (en) * 2014-07-25 2016-01-28 国立研究開発法人理化学研究所 Method for producing adenohypophysis or precursor tissue thereof

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Publication number Publication date
IL260380A (en) 2018-08-30
WO2017126551A1 (en) 2017-07-27
AU2017209742A1 (en) 2018-08-23
JP2022062165A (en) 2022-04-19
CA3013553A1 (en) 2017-07-27
EP3406709A4 (en) 2019-08-28
JPWO2017126551A1 (en) 2018-11-22
CN108495929B (en) 2023-04-18
KR20180101467A (en) 2018-09-12
MY194128A (en) 2022-11-14
US20190010452A1 (en) 2019-01-10
AU2017209742B2 (en) 2022-12-01
IL260380B2 (en) 2023-09-01
JP7023496B2 (en) 2022-02-22
EP3406709A1 (en) 2018-11-28
JP7465569B2 (en) 2024-04-11
IL260380B1 (en) 2023-05-01
CN108495929A (en) 2018-09-04

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