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SG11201609366TA - Alpha (1,2) fucosyltransferase syngenes for use in the production of fucosylated oligosaccharides - Google Patents

Alpha (1,2) fucosyltransferase syngenes for use in the production of fucosylated oligosaccharides

Info

Publication number
SG11201609366TA
SG11201609366TA SG11201609366TA SG11201609366TA SG11201609366TA SG 11201609366T A SG11201609366T A SG 11201609366TA SG 11201609366T A SG11201609366T A SG 11201609366TA SG 11201609366T A SG11201609366T A SG 11201609366TA SG 11201609366T A SG11201609366T A SG 11201609366TA
Authority
SG
Singapore
Prior art keywords
alpha
production
fucosylated oligosaccharides
syngenes
fucosyltransferase
Prior art date
Application number
SG11201609366TA
Inventor
John M Mccoy
Matthew Ian Heidtman
Massimo Merighi
Original Assignee
Glycosyn LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Glycosyn LLC filed Critical Glycosyn LLC
Publication of SG11201609366TA publication Critical patent/SG11201609366TA/en

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/04Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H13/00Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids
    • C07H13/02Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids by carboxylic acids
    • C07H13/04Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids by carboxylic acids having the esterifying carboxyl radicals attached to acyclic carbon atoms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1048Glycosyltransferases (2.4)
    • C12N9/1051Hexosyltransferases (2.4.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/18Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y204/00Glycosyltransferases (2.4)
    • C12Y204/01Hexosyltransferases (2.4.1)
    • C12Y204/01069Galactoside 2-alpha-L-fucosyltransferase (2.4.1.69)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y204/00Glycosyltransferases (2.4)
    • C12Y204/01Hexosyltransferases (2.4.1)
    • C12Y204/01086Glucosaminylgalactosylglucosylceramide beta-galactosyltransferase (2.4.1.86)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y204/00Glycosyltransferases (2.4)
    • C12Y204/01Hexosyltransferases (2.4.1)
    • C12Y204/01149N-Acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase (2.4.1.149)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
SG11201609366TA 2014-05-15 2015-05-14 Alpha (1,2) fucosyltransferase syngenes for use in the production of fucosylated oligosaccharides SG11201609366TA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461993742P 2014-05-15 2014-05-15
PCT/US2015/030823 WO2015175801A1 (en) 2014-05-15 2015-05-14 Alpha (1,2) fucosyltransferase syngenes for use in the production of fucosylated oligosaccharides

Publications (1)

Publication Number Publication Date
SG11201609366TA true SG11201609366TA (en) 2016-12-29

Family

ID=54480689

Family Applications (2)

Application Number Title Priority Date Filing Date
SG10202010599TA SG10202010599TA (en) 2014-05-15 2015-05-14 Alpha (1,2) fucosyltransferase syngenes for use in the production of fucosylated oligosaccharides
SG11201609366TA SG11201609366TA (en) 2014-05-15 2015-05-14 Alpha (1,2) fucosyltransferase syngenes for use in the production of fucosylated oligosaccharides

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SG10202010599TA SG10202010599TA (en) 2014-05-15 2015-05-14 Alpha (1,2) fucosyltransferase syngenes for use in the production of fucosylated oligosaccharides

Country Status (12)

Country Link
US (2) US11046984B2 (en)
EP (2) EP4293033A3 (en)
JP (3) JP2017515455A (en)
KR (2) KR20170035835A (en)
CN (1) CN106795484B (en)
AU (1) AU2015259088B2 (en)
CA (1) CA2945661C (en)
ES (1) ES2962258T3 (en)
HU (1) HUE064860T2 (en)
MX (1) MX2016014807A (en)
SG (2) SG10202010599TA (en)
WO (1) WO2015175801A1 (en)

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Publication number Priority date Publication date Assignee Title
US9567361B2 (en) 2011-05-13 2017-02-14 Glycosyn LLC Use of purified 2′-fucosyllactose, 3-fucosyllactose and lactodifucotetraose as prebiotics
US9029136B2 (en) * 2012-07-25 2015-05-12 Glycosyn LLC Alpha (1,2) fucosyltransferases suitable for use in the production of fucosylated oligosaccharides
DK2970872T3 (en) 2013-03-14 2019-01-02 Glycosyn LLC Microorganisms and Methods for Preparation of Sialylated and N-Acetylglucosamine-Containing Oligosaccharides
JP2017515455A (en) 2014-05-15 2017-06-15 グリコシン リミテッド ライアビリティー カンパニー Alpha (1,2) fucosyltransferase syngene for use in the production of fucosylated oligosaccharides
EP3390652A4 (en) * 2015-12-18 2019-06-12 Glycom A/S Fermentative production of oligosaccharides
US11572548B2 (en) 2015-12-18 2023-02-07 The Regents Of The University Of California Reaction mixture for synthesis of alpha1-2-fucosides
CN106190937B9 (en) * 2016-07-18 2020-12-01 南开大学 Method for biosynthesizing 2' -fucosyllactose by constructing recombinant escherichia coli
PL3375291T3 (en) * 2017-03-17 2022-11-28 Chr. Hansen HMO GmbH A method of inhibiting isomerization of a reducing saccharide upon thermal treatment
EP3425052A1 (en) * 2017-07-07 2019-01-09 Jennewein Biotechnologie GmbH Fucosyltransferases and their use in producing fucosylated oligosaccharides
KR101953375B1 (en) * 2017-11-20 2019-02-28 고려대학교 산학협력단 Method for producing various novel fucosylated oligosaccharides and use thereof
CN109402020B (en) * 2018-11-30 2020-08-04 江南大学 Lactobacillus helveticus strain capable of highly producing arginine aminopeptidase and application thereof
CN109735479B (en) * 2019-01-30 2022-04-01 光明乳业股份有限公司 Recombinant bacillus subtilis for synthesizing 2' -fucosyllactose and construction method and application thereof
HUE065545T2 (en) * 2019-03-04 2024-06-28 Aurealis Therapeutics AG Chloride-inducible prokaryotic expression system
JP7303296B2 (en) * 2019-06-05 2023-07-04 森永乳業株式会社 nutritional composition
CA3164948A1 (en) 2019-12-17 2021-06-24 Inbiose N.V. Lactose converting alpha-1,2-fucosyltransferase enzymes
CN111218488B (en) * 2020-02-10 2022-11-15 中国科学院上海高等研究院 Method for producing 2' -fucosyllactose by using escherichia coli
CN111471605B (en) * 2020-03-17 2022-03-08 山东大学 Saccharomyces cerevisiae engineering strain for high yield of fucosyllactose and application thereof
CN111471606B (en) * 2020-03-17 2022-03-08 山东大学 Optimized saccharomyces cerevisiae strain capable of producing fucosyllactose at high yield and application thereof
KR102719035B1 (en) * 2020-04-20 2024-10-18 주식회사 리비옴 Microorganism expressing vasoactive intestinal peptide, and use thereof
EP3954769A1 (en) * 2020-08-10 2022-02-16 Inbiose N.V. Production of oligosaccharide mixtures by a cell
CA3178744C (en) 2020-05-13 2023-11-14 Ardythe L. Morrow Fucosylated oligosaccharides for prevention of coronavirus infection
CA3178736A1 (en) 2020-05-13 2021-11-18 Ardythe L. Morrow 2'-fucosyllactose for the prevention and treatment of coronavirus-induced inflammation
EP4192972A1 (en) 2020-08-10 2023-06-14 Inbiose N.V. Production of glcnac containing bioproducts in a cell
JP2023537880A (en) * 2020-08-19 2023-09-06 コナゲン インコーポレイテッド Biosynthetic production of 2-fucosyllactose
KR102633804B1 (en) * 2020-12-31 2024-02-05 주식회사 삼양사 Recombinant Bacillus genus microorganism and Method for producing human milk oligosaccharides using the same
KR20220096752A (en) 2020-12-31 2022-07-07 주식회사 삼양사 Recombinant microorganism expressing fucosyltransferase and Method of producing 2’-fucolsylactose using thereof
WO2023182970A1 (en) * 2021-02-19 2023-09-28 The Regents Of The University Of California Methods for production of fucosylated oligosaccharides in recombinant cell culture
AU2022312712A1 (en) * 2021-07-16 2024-02-22 Inbiose N.V. Lacto-n-biose converting fucosyltransferases
CN113817701B (en) * 2021-09-30 2023-08-18 中南大学 Alpha-1, 2-fucosyltransferase mutant and application thereof
CN113832092B (en) * 2021-10-20 2023-10-03 江南大学 Genetically engineered bacterium for improving lactoyl-N-fucose yield and production method thereof
CN116286919A (en) * 2021-12-03 2023-06-23 虹摹生物科技(上海)有限公司 Genetically engineered bacterium and method for preparing fucosylated oligosaccharides by using genetically engineered bacterium
KR20240017427A (en) * 2022-07-29 2024-02-08 주식회사 진켐 Recombinant microorganism for producing 3'-fucosyllactose and methods using the same
CN116042671A (en) * 2023-02-14 2023-05-02 华熙生物科技股份有限公司 Nucleic acid molecule, expression vector and microorganism for preparing breast milk oligosaccharide
CN116769808A (en) * 2023-02-27 2023-09-19 江南大学 Strain for specifically producing 2' -fucosyllactose and application thereof

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FR2796082B1 (en) * 1999-07-07 2003-06-27 Centre Nat Rech Scient PROCESS FOR PRODUCING OLIGOSACCHARIDES
AU2009296507A1 (en) 2008-09-25 2010-04-01 Glycosyn LLC Compositions and methods for engineering probiotic yeast
ES2439507T3 (en) 2011-01-20 2014-01-23 Jennewein Biotechnologie Gmbh Novel fucosyltransferases and their applications
EP2675899A4 (en) * 2011-02-16 2014-09-10 Glycosyn LLC Biosynthesis of human milk oligosaccharides in engineered bacteria
US9029136B2 (en) * 2012-07-25 2015-05-12 Glycosyn LLC Alpha (1,2) fucosyltransferases suitable for use in the production of fucosylated oligosaccharides
ES2715010T3 (en) * 2014-03-31 2019-05-31 Jennewein Biotechnologie Gmbh Total oligosaccharide fermentation
JP2017515455A (en) 2014-05-15 2017-06-15 グリコシン リミテッド ライアビリティー カンパニー Alpha (1,2) fucosyltransferase syngene for use in the production of fucosylated oligosaccharides

Also Published As

Publication number Publication date
US20170081353A1 (en) 2017-03-23
KR20170035835A (en) 2017-03-31
EP4293033A3 (en) 2024-04-10
HUE064860T2 (en) 2024-04-28
CN106795484B (en) 2022-09-09
US11046984B2 (en) 2021-06-29
US11643675B2 (en) 2023-05-09
AU2015259088A2 (en) 2018-06-28
WO2015175801A9 (en) 2016-10-20
EP3083938B1 (en) 2023-11-08
ES2962258T3 (en) 2024-03-18
WO2015175801A1 (en) 2015-11-19
NZ722185A (en) 2023-12-22
JP2020188761A (en) 2020-11-26
EP3083938A4 (en) 2017-05-31
KR20230113650A (en) 2023-07-31
EP3083938A1 (en) 2016-10-26
JP2022173457A (en) 2022-11-18
SG10202010599TA (en) 2020-11-27
CA2945661C (en) 2023-10-17
US20220056497A1 (en) 2022-02-24
MX2016014807A (en) 2017-05-10
AU2015259088A1 (en) 2016-08-04
CN106795484A (en) 2017-05-31
CA2945661A1 (en) 2015-11-19
AU2015259088B2 (en) 2021-11-11
EP4293033A2 (en) 2023-12-20
JP2017515455A (en) 2017-06-15
EP3083938C0 (en) 2023-11-08

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