SG10201911905QA - Analysis system for orthogonal access to and tagging of biomolecules in cellular compartments - Google Patents
Analysis system for orthogonal access to and tagging of biomolecules in cellular compartmentsInfo
- Publication number
- SG10201911905QA SG10201911905QA SG10201911905QA SG10201911905QA SG10201911905QA SG 10201911905Q A SG10201911905Q A SG 10201911905QA SG 10201911905Q A SG10201911905Q A SG 10201911905QA SG 10201911905Q A SG10201911905Q A SG 10201911905QA SG 10201911905Q A SG10201911905Q A SG 10201911905QA
- Authority
- SG
- Singapore
- Prior art keywords
- biomolecules
- tagging
- analysis system
- cellular compartments
- orthogonal access
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6804—Nucleic acid analysis using immunogens
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1089—Design, preparation, screening or analysis of libraries using computer algorithms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Pathology (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662440089P | 2016-12-29 | 2016-12-29 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG10201911905QA true SG10201911905QA (en) | 2020-01-30 |
Family
ID=61007854
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG10201911905QA SG10201911905QA (en) | 2016-12-29 | 2017-12-28 | Analysis system for orthogonal access to and tagging of biomolecules in cellular compartments |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20190323003A1 (en) |
| EP (1) | EP3565904A1 (en) |
| JP (3) | JP7234114B2 (en) |
| KR (2) | KR20230104772A (en) |
| CN (1) | CN110431237A (en) |
| AU (2) | AU2017386533B2 (en) |
| CA (1) | CA3048863A1 (en) |
| SG (1) | SG10201911905QA (en) |
| WO (1) | WO2018125982A1 (en) |
Families Citing this family (41)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10400280B2 (en) | 2012-08-14 | 2019-09-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| MX364957B (en) | 2012-08-14 | 2019-05-15 | 10X Genomics Inc | Microcapsule compositions and methods. |
| US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| EP2931919B1 (en) | 2012-12-14 | 2019-02-20 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| CN108753766A (en) | 2013-02-08 | 2018-11-06 | 10X基因组学有限公司 | Polynucleotides bar code generating at |
| DE202015009609U1 (en) | 2014-04-10 | 2018-08-06 | 10X Genomics, Inc. | Microfluidic system for the production of emulsions |
| KR102531677B1 (en) | 2014-06-26 | 2023-05-10 | 10엑스 제노믹스, 인크. | Methods of analyzing nucleic acids from individual cells or cell populations |
| CN112126675B (en) | 2015-01-12 | 2022-09-09 | 10X基因组学有限公司 | Method and system for preparing nucleic acid sequencing library and library prepared by using same |
| EP4286516A3 (en) | 2015-02-24 | 2024-03-06 | 10X Genomics, Inc. | Partition processing methods and systems |
| US11371094B2 (en) | 2015-11-19 | 2022-06-28 | 10X Genomics, Inc. | Systems and methods for nucleic acid processing using degenerate nucleotides |
| US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| WO2018140966A1 (en) | 2017-01-30 | 2018-08-02 | 10X Genomics, Inc. | Methods and systems for droplet-based single cell barcoding |
| US10995333B2 (en) | 2017-02-06 | 2021-05-04 | 10X Genomics, Inc. | Systems and methods for nucleic acid preparation |
| US10844372B2 (en) | 2017-05-26 | 2020-11-24 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| CN116064732A (en) | 2017-05-26 | 2023-05-05 | 10X基因组学有限公司 | Single cell analysis of transposase accessibility chromatin |
| SG11201913654QA (en) | 2017-11-15 | 2020-01-30 | 10X Genomics Inc | Functionalized gel beads |
| US10829815B2 (en) | 2017-11-17 | 2020-11-10 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
| EP3752832A1 (en) | 2018-02-12 | 2020-12-23 | 10X Genomics, Inc. | Methods characterizing multiple analytes from individual cells or cell populations |
| EP3775271A1 (en) | 2018-04-06 | 2021-02-17 | 10X Genomics, Inc. | Systems and methods for quality control in single cell processing |
| WO2019217758A1 (en) | 2018-05-10 | 2019-11-14 | 10X Genomics, Inc. | Methods and systems for molecular library generation |
| FI3810774T3 (en) * | 2018-06-04 | 2023-12-11 | Illumina Inc | Methods of making high-throughput single-cell transcriptome libraries |
| US20200032335A1 (en) | 2018-07-27 | 2020-01-30 | 10X Genomics, Inc. | Systems and methods for metabolome analysis |
| US12065688B2 (en) | 2018-08-20 | 2024-08-20 | 10X Genomics, Inc. | Compositions and methods for cellular processing |
| US11459607B1 (en) | 2018-12-10 | 2022-10-04 | 10X Genomics, Inc. | Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes |
| US11845983B1 (en) | 2019-01-09 | 2023-12-19 | 10X Genomics, Inc. | Methods and systems for multiplexing of droplet based assays |
| SG11202012753SA (en) | 2019-01-29 | 2021-01-28 | Illumina Inc | Sequencing kits |
| MX2020013413A (en) | 2019-01-29 | 2021-04-28 | Illumina Inc | Flow cells. |
| TW202100247A (en) | 2019-01-29 | 2021-01-01 | 美商伊路米納有限公司 | Flow cells |
| US11851683B1 (en) | 2019-02-12 | 2023-12-26 | 10X Genomics, Inc. | Methods and systems for selective analysis of cellular samples |
| MX2022001468A (en) | 2019-08-09 | 2022-07-19 | Nutcracker Therapeutics Inc | Methods and apparatuses for manufacturing for removing material from a therapeutic composition. |
| WO2021146184A1 (en) | 2020-01-13 | 2021-07-22 | Fluent Biosciences Inc. | Single cell sequencing |
| US20240263227A1 (en) * | 2020-02-17 | 2024-08-08 | Universal Sequencing Technology Corporation | Methods of barcoding nucleic acid for detection and sequencing |
| US11866782B2 (en) | 2020-03-16 | 2024-01-09 | Fluent Biosciences Inc. | Multi-omic analysis in monodisperse droplets |
| WO2022015970A1 (en) * | 2020-07-15 | 2022-01-20 | Fluent Biosciences Inc. | Tiered ligation oligos |
| JPWO2022118894A1 (en) * | 2020-12-02 | 2022-06-09 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CA2256128A1 (en) | 1998-12-29 | 2000-06-29 | Stephen William Davies | Coded dna processing |
| US20060134629A1 (en) * | 2000-03-20 | 2006-06-22 | Link Charles J | Methods and compositions for elucidating protein expression profiles in cells |
| TW200510723A (en) | 2003-07-15 | 2005-03-16 | Bioarray Solutions Ltd | Concurrent optimization in selection of primer and capture probe sets for nucleic acid analysis |
| WO2009041831A1 (en) * | 2007-09-25 | 2009-04-02 | Pastoral Greenhouse Gas Research Limited | PHAGE ϕ-MRU POLYNUCLEOTIDES AND POLYPEPTIDES AND USES THEREOF |
| US9080211B2 (en) | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| EP2359289A4 (en) | 2008-11-26 | 2015-11-11 | Illumina Inc | Methods and systems for analysis of sequencing data |
| US20100323348A1 (en) | 2009-01-31 | 2010-12-23 | The Regents Of The University Of Colorado, A Body Corporate | Methods and Compositions for Using Error-Detecting and/or Error-Correcting Barcodes in Nucleic Acid Amplification Process |
| WO2012061832A1 (en) | 2010-11-05 | 2012-05-10 | Illumina, Inc. | Linking sequence reads using paired code tags |
| US9074251B2 (en) | 2011-02-10 | 2015-07-07 | Illumina, Inc. | Linking sequence reads using paired code tags |
| US8829171B2 (en) | 2011-02-10 | 2014-09-09 | Illumina, Inc. | Linking sequence reads using paired code tags |
| US9365897B2 (en) | 2011-02-08 | 2016-06-14 | Illumina, Inc. | Selective enrichment of nucleic acids |
| US9683230B2 (en) | 2013-01-09 | 2017-06-20 | Illumina Cambridge Limited | Sample preparation on a solid support |
| CN105339503B (en) * | 2013-05-23 | 2020-04-10 | 斯坦福大学托管董事会 | Transposition to native chromatin for personal epigenomics |
| US10017759B2 (en) | 2014-06-26 | 2018-07-10 | Illumina, Inc. | Library preparation of tagged nucleic acid |
| KR101484042B1 (en) | 2014-07-23 | 2015-01-19 | 국방과학연구소 | Manufacturing method for thin metal foam impregnated with lithium as an anode for thermally activated reserve batteries |
| EP4239067A3 (en) * | 2014-11-05 | 2023-10-25 | Illumina, Inc. | Transposase compositions for reduction of insertion bias |
| CA2975739C (en) * | 2015-02-10 | 2022-12-06 | Illumina, Inc. | Methods and compositions for analyzing cellular components |
-
2017
- 2017-12-28 EP EP17832699.7A patent/EP3565904A1/en active Pending
- 2017-12-28 WO PCT/US2017/068672 patent/WO2018125982A1/en unknown
- 2017-12-28 CA CA3048863A patent/CA3048863A1/en active Pending
- 2017-12-28 JP JP2019535278A patent/JP7234114B2/en active Active
- 2017-12-28 KR KR1020237022198A patent/KR20230104772A/en not_active Application Discontinuation
- 2017-12-28 CN CN201780087116.5A patent/CN110431237A/en active Pending
- 2017-12-28 SG SG10201911905QA patent/SG10201911905QA/en unknown
- 2017-12-28 KR KR1020197021415A patent/KR102551666B1/en active IP Right Grant
- 2017-12-28 AU AU2017386533A patent/AU2017386533B2/en active Active
-
2019
- 2019-06-28 US US16/456,763 patent/US20190323003A1/en active Pending
-
2022
- 2022-03-28 JP JP2022051494A patent/JP7455886B2/en active Active
-
2024
- 2024-02-06 JP JP2024016433A patent/JP2024054221A/en active Pending
- 2024-07-18 AU AU2024204952A patent/AU2024204952A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| AU2017386533B2 (en) | 2024-05-09 |
| RU2019123065A (en) | 2021-02-01 |
| RU2019123065A3 (en) | 2021-10-13 |
| JP2022088511A (en) | 2022-06-14 |
| JP2024054221A (en) | 2024-04-16 |
| JP2020506671A (en) | 2020-03-05 |
| EP3565904A1 (en) | 2019-11-13 |
| KR20230104772A (en) | 2023-07-10 |
| CA3048863A1 (en) | 2018-07-05 |
| CN110431237A (en) | 2019-11-08 |
| AU2017386533A1 (en) | 2019-07-18 |
| JP7455886B2 (en) | 2024-03-26 |
| US20190323003A1 (en) | 2019-10-24 |
| JP7234114B2 (en) | 2023-03-07 |
| KR102551666B1 (en) | 2023-07-04 |
| AU2024204952A1 (en) | 2024-08-08 |
| KR20190097239A (en) | 2019-08-20 |
| WO2018125982A1 (en) | 2018-07-05 |
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