SG10201805453XA - Multiplexed analysis of target nucleic acids - Google Patents
Multiplexed analysis of target nucleic acidsInfo
- Publication number
- SG10201805453XA SG10201805453XA SG10201805453XA SG10201805453XA SG10201805453XA SG 10201805453X A SG10201805453X A SG 10201805453XA SG 10201805453X A SG10201805453X A SG 10201805453XA SG 10201805453X A SG10201805453X A SG 10201805453XA SG 10201805453X A SG10201805453X A SG 10201805453XA
- Authority
- SG
- Singapore
- Prior art keywords
- target nucleic
- nucleic acids
- amplified
- captured
- sample
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/682—Signal amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Multiplexed Analysis of Target Nucleic Acids The present invention provides, among other things, methods of detecting target nucleic acid, comprising steps of: a) contacting a sample with one or more capturing probes, each comprising at least one target capturing sequence, under conditions that permit the one or more capturing probes to capture one or more target nucleic acids in the sample; b) amplifying the captured one or more target nucleic acids in a reaction mixture comprising a detectable entity such that the amplified one or more target nucleic acids are labeled with the detectable entity; c) incubating amplification product with a plurality of re-capturing probes such that the amplified one or more target nucleic acids are re-captured by the plurality of the re-capturing probes; and d) detecting signal generated by detectable entity associated with the re-captured amplified one or more target nucleic acids, wherein the presence and/or abundance of the detectable signal indicates the presence and/or abundance of the one or more target nucleic acids in the sample. [No Suitable Figure]
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361816070P | 2013-04-25 | 2013-04-25 | |
| US201461936826P | 2014-02-06 | 2014-02-06 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG10201805453XA true SG10201805453XA (en) | 2018-08-30 |
Family
ID=51792423
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG10201805453XA SG10201805453XA (en) | 2013-04-25 | 2014-04-25 | Multiplexed analysis of target nucleic acids |
| SG11201508460TA SG11201508460TA (en) | 2013-04-25 | 2014-04-25 | Multiplexed analysis of target nucleic acids |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201508460TA SG11201508460TA (en) | 2013-04-25 | 2014-04-25 | Multiplexed analysis of target nucleic acids |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US20160060682A1 (en) |
| EP (1) | EP2989215B1 (en) |
| JP (1) | JP6633514B2 (en) |
| CN (1) | CN105164279B (en) |
| AU (1) | AU2014256903B2 (en) |
| CA (1) | CA2909861C (en) |
| SG (2) | SG10201805453XA (en) |
| WO (1) | WO2014176575A1 (en) |
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| CN106715768B (en) | 2014-07-30 | 2020-06-16 | 哈佛学院院长及董事 | System and method for assaying nucleic acids |
| EP3262192B1 (en) | 2015-02-27 | 2020-09-16 | Becton, Dickinson and Company | Spatially addressable molecular barcoding |
| US11535882B2 (en) | 2015-03-30 | 2022-12-27 | Becton, Dickinson And Company | Methods and compositions for combinatorial barcoding |
| WO2016172373A1 (en) | 2015-04-23 | 2016-10-27 | Cellular Research, Inc. | Methods and compositions for whole transcriptome amplification |
| CN107636167B (en) * | 2015-05-18 | 2022-09-02 | 传奇诊断股份公司 | Detection of target nucleic acids and variants |
| EP3347714B1 (en) * | 2015-09-10 | 2021-03-10 | InSilixa Inc. | Methods for multiplex quantitative nucleic acid amplification |
| WO2017044574A1 (en) | 2015-09-11 | 2017-03-16 | Cellular Research, Inc. | Methods and compositions for nucleic acid library normalization |
| CN108368547B (en) | 2015-12-15 | 2022-04-05 | Seegene株式会社 | Signal extraction in relation to target nucleic acid sequences |
| US20210317540A1 (en) * | 2015-12-28 | 2021-10-14 | Pathogendx, Inc. | Microarray-Based Multiplex Fungal Pathogen Analysis |
| WO2017131463A1 (en) * | 2016-01-26 | 2017-08-03 | Seegene, Inc. . | Methods for providing signal for target nucleic acid sequence |
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| US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
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| EP3516400B1 (en) | 2016-09-26 | 2023-08-16 | Becton, Dickinson and Company | Measurement of protein expression using reagents with barcoded oligonucleotide sequences |
| CN116242687A (en) * | 2016-11-08 | 2023-06-09 | 哈佛学院院长及董事 | Multiplex imaging using MERFISH, extended microscopy and related techniques |
| EP3577232A1 (en) | 2017-02-01 | 2019-12-11 | Cellular Research, Inc. | Selective amplification using blocking oligonucleotides |
| JP7062681B2 (en) * | 2017-03-20 | 2022-05-06 | イルミナ インコーポレイテッド | Methods and Compositions for Preparing Nucleic Acid Libraries |
| CN110475871B (en) | 2017-03-28 | 2024-04-12 | Seegene株式会社 | Analytical signal for determining the presence of a target nucleic acid sequence |
| US11788123B2 (en) | 2017-05-26 | 2023-10-17 | President And Fellows Of Harvard College | Systems and methods for high-throughput image-based screening |
| JP7536450B2 (en) | 2017-06-05 | 2024-08-20 | ベクトン・ディキンソン・アンド・カンパニー | Single cell sample indexing |
| KR102372572B1 (en) * | 2017-08-04 | 2022-03-08 | 빌리언투원, 인크. | Measurement and analysis of sequencing output using target-associated molecules in quantification associated with biological targets |
| ES2702432B2 (en) * | 2017-08-31 | 2019-08-05 | Venegas Pedro Manuel Medina | Method and device for nucleic acid analysis |
| US20200399683A1 (en) * | 2018-02-26 | 2020-12-24 | Cy Molecular Diagnostics, Inc. | Composition and Methods For Affinity Directed Enrichment of Rare Species |
| WO2019199579A1 (en) * | 2018-04-09 | 2019-10-17 | The Board Of Trustees Of The Leland Stanford Junior University | Method of in situ gene sequencing |
| JP7358388B2 (en) | 2018-05-03 | 2023-10-10 | ベクトン・ディキンソン・アンド・カンパニー | Molecular barcoding at opposite transcript ends |
| CN112272710A (en) | 2018-05-03 | 2021-01-26 | 贝克顿迪金森公司 | High throughput omics sample analysis |
| US12049665B2 (en) | 2018-06-12 | 2024-07-30 | Accuragen Holdings Limited | Methods and compositions for forming ligation products |
| WO2020072380A1 (en) | 2018-10-01 | 2020-04-09 | Cellular Research, Inc. | Determining 5' transcript sequences |
| KR20210084441A (en) * | 2018-10-25 | 2021-07-07 | 일루미나, 인코포레이티드 | Methods and compositions for identifying ligands using indexes and barcodes on arrays |
| CN112969789A (en) | 2018-11-08 | 2021-06-15 | 贝克顿迪金森公司 | Single cell whole transcriptome analysis using random priming |
| CN113195717A (en) | 2018-12-13 | 2021-07-30 | 贝克顿迪金森公司 | Selective extension in single cell whole transcriptome analysis |
| CN113574178B (en) | 2019-01-23 | 2024-10-29 | 贝克顿迪金森公司 | Oligonucleotides associated with antibodies |
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| CN110057797B (en) * | 2019-04-24 | 2021-05-18 | 南京工业大学 | Method for detecting microRNA-155 based on mesh structure constructed by quantum dots |
| EP4004231A1 (en) | 2019-07-22 | 2022-06-01 | Becton, Dickinson and Company | Single cell chromatin immunoprecipitation sequencing assay |
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| WO2024106421A1 (en) * | 2022-11-15 | 2024-05-23 | 学校法人関西医科大学 | Method for collecting mirna, collection membrane filter, collection membrane filter unit, and collection kit |
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| US6001571A (en) * | 1995-11-30 | 1999-12-14 | Mandecki; Wlodek | Multiplex assay for nucleic acids employing transponders |
| US7622294B2 (en) * | 1997-03-14 | 2009-11-24 | Trustees Of Tufts College | Methods for detecting target analytes and enzymatic reactions |
| WO2001083814A2 (en) * | 2000-05-04 | 2001-11-08 | Syngenta Participations Ag | Assay for nucleic acid analysis |
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-
2014
- 2014-04-25 SG SG10201805453XA patent/SG10201805453XA/en unknown
- 2014-04-25 AU AU2014256903A patent/AU2014256903B2/en not_active Ceased
- 2014-04-25 CN CN201480022755.XA patent/CN105164279B/en active Active
- 2014-04-25 EP EP14788247.6A patent/EP2989215B1/en active Active
- 2014-04-25 SG SG11201508460TA patent/SG11201508460TA/en unknown
- 2014-04-25 JP JP2016510819A patent/JP6633514B2/en not_active Expired - Fee Related
- 2014-04-25 WO PCT/US2014/035578 patent/WO2014176575A1/en active Application Filing
- 2014-04-25 US US14/784,531 patent/US20160060682A1/en not_active Abandoned
- 2014-04-25 CA CA2909861A patent/CA2909861C/en active Active
-
2022
- 2022-07-11 US US17/861,961 patent/US20230193352A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| JP2016516437A (en) | 2016-06-09 |
| US20230193352A1 (en) | 2023-06-22 |
| EP2989215A1 (en) | 2016-03-02 |
| EP2989215B1 (en) | 2019-09-04 |
| CA2909861A1 (en) | 2014-10-30 |
| WO2014176575A1 (en) | 2014-10-30 |
| AU2014256903B2 (en) | 2020-09-24 |
| US20160060682A1 (en) | 2016-03-03 |
| CN105164279A (en) | 2015-12-16 |
| AU2014256903A1 (en) | 2015-09-17 |
| CN105164279B (en) | 2021-02-02 |
| SG11201508460TA (en) | 2015-11-27 |
| EP2989215A4 (en) | 2016-12-28 |
| CA2909861C (en) | 2022-12-06 |
| JP6633514B2 (en) | 2020-01-22 |
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