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SG10201704426UA - Peg-mediated assembly of nucleic acid molecules - Google Patents

Peg-mediated assembly of nucleic acid molecules

Info

Publication number
SG10201704426UA
SG10201704426UA SG10201704426UA SG10201704426UA SG10201704426UA SG 10201704426U A SG10201704426U A SG 10201704426UA SG 10201704426U A SG10201704426U A SG 10201704426UA SG 10201704426U A SG10201704426U A SG 10201704426UA SG 10201704426U A SG10201704426U A SG 10201704426UA
Authority
SG
Singapore
Prior art keywords
peg
nucleic acid
acid molecules
mediated assembly
mediated
Prior art date
Application number
SG10201704426UA
Inventor
Jun Urano
Daniel G Gibson
Nicky C Caiazza
Zhiqing Qi
Original Assignee
Synthetic Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Synthetic Genomics Inc filed Critical Synthetic Genomics Inc
Publication of SG10201704426UA publication Critical patent/SG10201704426UA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • C12N15/1027Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • C12N15/1031Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
SG10201704426UA 2012-12-13 2013-12-11 Peg-mediated assembly of nucleic acid molecules SG10201704426UA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201261736946P 2012-12-13 2012-12-13

Publications (1)

Publication Number Publication Date
SG10201704426UA true SG10201704426UA (en) 2017-06-29

Family

ID=50934928

Family Applications (2)

Application Number Title Priority Date Filing Date
SG11201504584VA SG11201504584VA (en) 2012-12-13 2013-12-11 Peg-mediated assembly of nucleic acid molecules
SG10201704426UA SG10201704426UA (en) 2012-12-13 2013-12-11 Peg-mediated assembly of nucleic acid molecules

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SG11201504584VA SG11201504584VA (en) 2012-12-13 2013-12-11 Peg-mediated assembly of nucleic acid molecules

Country Status (10)

Country Link
US (2) US20140308710A1 (en)
EP (1) EP2931913B1 (en)
JP (3) JP2015536689A (en)
CN (2) CN104822840A (en)
AU (1) AU2013359293B2 (en)
CA (1) CA2893817C (en)
DK (1) DK2931913T3 (en)
IL (1) IL238918B (en)
SG (2) SG11201504584VA (en)
WO (1) WO2014093535A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2643356A1 (en) 2005-12-06 2008-02-28 J. Craig Venter Institute Synthetic genomes
EP2931913B1 (en) * 2012-12-13 2018-10-17 Synthetic Genomics, Inc. Peg-mediated assembly of nucleic acid molecules
JPWO2019044820A1 (en) * 2017-08-28 2020-12-10 Spiber株式会社 Method for Producing Double-stranded DNA Fragment
JP2019198236A (en) * 2018-05-14 2019-11-21 国立大学法人神戸大学 Double-stranded DNA synthesis method
MX2021005028A (en) 2018-10-31 2021-08-24 Zymergen Inc Multiplexed deterministic assembly of dna libraries.
EP4114936A4 (en) 2020-03-03 2024-03-27 Codex Dna, Inc. Methods for assembling nucleic acids

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5928905A (en) * 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
US5834252A (en) * 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US7399590B2 (en) * 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US20090305233A1 (en) * 2007-07-03 2009-12-10 Arizona Board Of Regents, A Body Corporate Of The State Of Arizona Methods and Reagents for Polynucleotide Assembly
JP2009284896A (en) * 2007-07-26 2009-12-10 Fujifilm Corp Nucleic acid amplification method
AU2008307153B2 (en) * 2007-10-04 2015-03-12 Commonwealth Scientific And Industrial Research Organisation Nucleic acid amplification
EP2207899B1 (en) * 2007-10-08 2018-04-18 Synthetic Genomics, Inc. Assembly of large nucleic acids
CN102016070A (en) * 2008-02-15 2011-04-13 合成基因组公司 Methods for in vitro joining and combinatorial assembly of nucleic acid molecules
EP2756098B1 (en) * 2011-09-16 2018-06-06 Lexogen GmbH Method for making library of nucleic acid molecules
IL288256B2 (en) * 2012-08-16 2023-10-01 Synthetic Genomics Inc Digital to biological converter
EP2931913B1 (en) * 2012-12-13 2018-10-17 Synthetic Genomics, Inc. Peg-mediated assembly of nucleic acid molecules
EP3666904A1 (en) * 2018-12-14 2020-06-17 Lexogen GmbH Nucleic acid amplification and identification method

Also Published As

Publication number Publication date
US20190177759A1 (en) 2019-06-13
JP7009337B2 (en) 2022-01-25
JP2019022503A (en) 2019-02-14
US20140308710A1 (en) 2014-10-16
AU2013359293A1 (en) 2015-06-18
CN104822840A (en) 2015-08-05
SG11201504584VA (en) 2015-07-30
JP2021048883A (en) 2021-04-01
WO2014093535A8 (en) 2015-07-02
CA2893817A1 (en) 2014-06-19
EP2931913B1 (en) 2018-10-17
IL238918A0 (en) 2015-07-30
WO2014093535A1 (en) 2014-06-19
CN109913519A (en) 2019-06-21
JP2015536689A (en) 2015-12-24
EP2931913A4 (en) 2016-08-17
CN109913519B (en) 2022-12-02
DK2931913T3 (en) 2018-11-26
CA2893817C (en) 2021-03-09
IL238918B (en) 2020-04-30
AU2013359293B2 (en) 2017-11-02
EP2931913A1 (en) 2015-10-21

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