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SE8703733L - METHOD OF ANALYSIS FOR DETERMINING PERCENTAGE OF FREE SUBSTANCE IN BIOLOGICAL SCIENCES THROUGH EQUAL DISTRIBUTION IN OIL / WATER SYSTEM - Google Patents

METHOD OF ANALYSIS FOR DETERMINING PERCENTAGE OF FREE SUBSTANCE IN BIOLOGICAL SCIENCES THROUGH EQUAL DISTRIBUTION IN OIL / WATER SYSTEM

Info

Publication number
SE8703733L
SE8703733L SE8703733A SE8703733A SE8703733L SE 8703733 L SE8703733 L SE 8703733L SE 8703733 A SE8703733 A SE 8703733A SE 8703733 A SE8703733 A SE 8703733A SE 8703733 L SE8703733 L SE 8703733L
Authority
SE
Sweden
Prior art keywords
ligand
oil
free
present
analysis
Prior art date
Application number
SE8703733A
Other languages
Unknown language ( )
Swedish (sv)
Other versions
SE8703733D0 (en
Inventor
Ragnar Soedergaard
Original Assignee
Ragnar Soedergaard
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ragnar Soedergaard filed Critical Ragnar Soedergaard
Priority to SE8703733A priority Critical patent/SE8703733L/en
Publication of SE8703733D0 publication Critical patent/SE8703733D0/en
Priority to AU25321/88A priority patent/AU2532188A/en
Priority to PCT/SE1988/000500 priority patent/WO1989003043A1/en
Publication of SE8703733L publication Critical patent/SE8703733L/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/537—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody
    • G01N33/5375—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with separation of immune complex from unbound antigen or antibody by changing the physical or chemical properties of the medium or immunochemicals, e.g. temperature, density, pH, partitioning
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Endocrinology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention is based on the fact that an oil, e.g. corn oil, is not soluble in aqueous sample, e.g. a plasma sample. If the aqueous sample contains proteins which are natural binders for a ligand which have hydrophobic properties, then the ligand is present in the free state in both the oil phase and the water phase, while bound ligand is only present in the water phase. If a labeled version of the ligand is present in the system, it is possible to determine the percentage of free ligand in the aqueous sample under equilibrium conditions, provided that the partition coefficient of free ligand also is determined. The percentage of free ligand, together with the total concentration of the ligand, yields the free concentration of the ligand.
SE8703733A 1987-09-28 1987-09-28 METHOD OF ANALYSIS FOR DETERMINING PERCENTAGE OF FREE SUBSTANCE IN BIOLOGICAL SCIENCES THROUGH EQUAL DISTRIBUTION IN OIL / WATER SYSTEM SE8703733L (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
SE8703733A SE8703733L (en) 1987-09-28 1987-09-28 METHOD OF ANALYSIS FOR DETERMINING PERCENTAGE OF FREE SUBSTANCE IN BIOLOGICAL SCIENCES THROUGH EQUAL DISTRIBUTION IN OIL / WATER SYSTEM
AU25321/88A AU2532188A (en) 1987-09-28 1988-09-28 Assay for determining the percentage of free substances in biological fluids
PCT/SE1988/000500 WO1989003043A1 (en) 1987-09-28 1988-09-28 Assay for determining the percentage of free substances in biological fluids

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE8703733A SE8703733L (en) 1987-09-28 1987-09-28 METHOD OF ANALYSIS FOR DETERMINING PERCENTAGE OF FREE SUBSTANCE IN BIOLOGICAL SCIENCES THROUGH EQUAL DISTRIBUTION IN OIL / WATER SYSTEM

Publications (2)

Publication Number Publication Date
SE8703733D0 SE8703733D0 (en) 1987-09-28
SE8703733L true SE8703733L (en) 1989-03-29

Family

ID=20369691

Family Applications (1)

Application Number Title Priority Date Filing Date
SE8703733A SE8703733L (en) 1987-09-28 1987-09-28 METHOD OF ANALYSIS FOR DETERMINING PERCENTAGE OF FREE SUBSTANCE IN BIOLOGICAL SCIENCES THROUGH EQUAL DISTRIBUTION IN OIL / WATER SYSTEM

Country Status (3)

Country Link
AU (1) AU2532188A (en)
SE (1) SE8703733L (en)
WO (1) WO1989003043A1 (en)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL56839A0 (en) * 1979-03-11 1979-05-31 Technion Res & Dev Foundation Improvement in specific binding assay technique
GB2078370A (en) * 1980-06-13 1982-01-06 Nat Res Dev Binding assays
CA1292092C (en) * 1985-08-21 1991-11-12 Biotope, Inc. Methods and devices for separating and detecting components in specific binding assays

Also Published As

Publication number Publication date
AU2532188A (en) 1989-04-18
WO1989003043A1 (en) 1989-04-06
SE8703733D0 (en) 1987-09-28

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Effective date: 19900401