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RU94027451A - Method of plague microbe biomass preparing - Google Patents

Method of plague microbe biomass preparing

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Publication number
RU94027451A
RU94027451A RU94027451/13A RU94027451A RU94027451A RU 94027451 A RU94027451 A RU 94027451A RU 94027451/13 A RU94027451/13 A RU 94027451/13A RU 94027451 A RU94027451 A RU 94027451A RU 94027451 A RU94027451 A RU 94027451A
Authority
RU
Russia
Prior art keywords
plague
nutrient medium
plague microbe
cultivation
microbe
Prior art date
Application number
RU94027451/13A
Other languages
Russian (ru)
Other versions
RU2102472C1 (en
Inventor
А.Л. Ковтун
А.З. Рогожин
В.П. Непранов
Ю.Е. Нестеров
Н.А. Черкасов
Original Assignee
Научно-исследовательский институт микробиологии МО РФ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Научно-исследовательский институт микробиологии МО РФ filed Critical Научно-исследовательский институт микробиологии МО РФ
Priority to RU94027451A priority Critical patent/RU2102472C1/en
Publication of RU94027451A publication Critical patent/RU94027451A/en
Application granted granted Critical
Publication of RU2102472C1 publication Critical patent/RU2102472C1/en

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  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

FIELD: microbiological industry. SUBSTANCE: method involves submerged cultivation of microbe cells on the nutrient medium consisting of cultural fluid supernatant obtained from decantation stage of cell suspension cultivation and/or cultural fluid after plague vaccine semiproduct rejection and portion of fresh nutrient medium (15-20% of parental medium volume). Invention can be used for vaccine preparing and isolation of plague microbe cellular structures. EFFECT: decreased sewage volume, increased biomass yield.

Claims (1)

Изобретение относится к микробиологической промышленности, а именно к способам получения биомассы чумного микроба и может быть использовано при приготовлении вакцин или для выделения клеточных структур чумного микроба. Цель изобретения - сокращение объема сточных вод и повышение выхода биомассы. Способ получения биомассы чумного микроба включает в себя глубинное культивирование микробных клеток, где в качестве питательной среды используется смесь, состоящая из супернатанта культуральной жидкости, полученной со стадии декантации суспензии клеток после культивирования, и/или культуральной жидкости после выбраковки полуфабриката чумной вакцины по технологическим параметрам и порции свежей питательной среды, составляющей не менее 15 - 20% от исходного объема среды.The invention relates to the microbiological industry, and in particular to methods for producing plague microbe biomass and can be used in the preparation of vaccines or for isolation of plague microbe cell structures. The purpose of the invention is to reduce the volume of wastewater and increase the yield of biomass. The method for producing plague microbe biomass includes deep cultivation of microbial cells, where a mixture consisting of the supernatant of the culture fluid obtained from the stage of decantation of the cell suspension after cultivation, and / or the culture fluid after culling the semi-finished plague vaccine according to the technological parameters is used as a nutrient medium portions of fresh nutrient medium, comprising at least 15 - 20% of the initial volume of the medium.
RU94027451A 1994-07-19 1994-07-19 Method of plague microbe biomass preparing RU2102472C1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
RU94027451A RU2102472C1 (en) 1994-07-19 1994-07-19 Method of plague microbe biomass preparing

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
RU94027451A RU2102472C1 (en) 1994-07-19 1994-07-19 Method of plague microbe biomass preparing

Publications (2)

Publication Number Publication Date
RU94027451A true RU94027451A (en) 1996-09-27
RU2102472C1 RU2102472C1 (en) 1998-01-20

Family

ID=20158762

Family Applications (1)

Application Number Title Priority Date Filing Date
RU94027451A RU2102472C1 (en) 1994-07-19 1994-07-19 Method of plague microbe biomass preparing

Country Status (1)

Country Link
RU (1) RU2102472C1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012076441A1 (en) 2010-12-07 2012-06-14 F. Hoffmann-La Roche Ag Feed mixing device and its use
CN102634457A (en) * 2012-04-28 2012-08-15 徐长欣 Semi-finished culture medium and preparation method thereof

Also Published As

Publication number Publication date
RU2102472C1 (en) 1998-01-20

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