RU2626543C2 - Paenibacillus mucilaginosus bacteria strain, method for plants growth stimulation and protection against diseases and application of paenibacillus mucilaginosus bacteria strain as fertiliser and biological control agent (antipatogenic means) in prevention and/or treatment of plant disease - Google Patents

Abstract

FIELD: biotechnology.

SUBSTANCE: paenibacillus mucilaginosus strain, which stimulates the growth of plants and possesses an enhanced ability to synthesise bactericins with a wide spectrum of phytopathogenic microflora suppression, was deposited in the All-Russian Collection of Industrial Microorganisms under the registration number VKPM V-12259. Application of Paenibacillus mucilaginosus VKPM B-12259 strain as a fertiliser for crops and for prevention and treatment of plants fungal diseases. A method for growth stimulation and plants protection against fungal diseases is proposed. It presupposes pre-sowing treatment of soil, seeds, and culture suspension of Paenibacillus mucilaginosus VKPM B-12259 strain at a concentration not less than 120 million spores/ml.

EFFECT: increased yield of crops and their resistance to fungal diseases.

6 cl, 2 tbl, 5 ex

Description

FIELD OF THE INVENTION

The invention relates to agricultural microbiology, in particular to the bacterial strain Paenibacillus mucilaginosus Pm 2906 (registration number VKPM B-12259) used as a fertilizer, a method of stimulating the growth and protection of plants from diseases and the use of the strain Paenibacillus mucilaginosus Pm 2906 as a fertilizer and biological agent control in the prevention and / or treatment of plant diseases.

State of the art

Currently, the use of bacterial preparations to increase soil fertility is one of the techniques of agricultural technology, an alternative to the increasing use of mineral fertilizers. This is especially true in conditions of the need for environmental safety.

Bioadditives accelerate the process of release of nutrients from organic fertilizers, as well as the process of assimilation of released substances by plants. In addition, bioadditives contribute to the additional accumulation of nutrients in the soil in a form easily digestible by plants.

The use of bacterial fertilizers based on various strains of microorganisms or their metabolites is known.

Thus, the introduction of a mixture of peat, lime and mineral additives in an aqueous suspension of bacterial cultures (bacterial uterine culture of AMB and azobacterin) increases the effectiveness of the fertilizer (author. Certificate. 589238).

It is known that organomineral fertilizers are introduced to increase their effectiveness in a suspension of yeast and unicellular algae (author certificate 935501), a consortium of bacteria Streptococcus thermophilus, Streptococcus bovis, Dactobacillus salivaries var salicinicus, Lactobacillus salivaries var salicinicus, Lactobus bacteriophosphoric acid bacteria 20 (Azotobacter chroococcum), phosphate-soluble (Bacillus mucilaginosus), lactic acid in the form of a consortium containing Streptococcus thermophilus, Streptococcus bovis, Lactobacillus salivaries var salicinicus, Lactobacillus salivaries var salivarus 18, lactobacillus phosphorius 20, pat.

Fertilizers are known for the preparation of which strains grown on nutrient media containing peptone (RF patent 2241692) or nitrogenous compounds (author certificate. 1756318) are used, which contributes to an increase in the vegetative mass of plants as applied to fertilizers based on the Bacillus mucilaginosus and Agrobacterium radiobacter strain, respectively . The use of a biological product to increase crop yields and improve the quality of products containing, in addition to lactic acid, photosynthetic, nitrogen-containing bacteria, strains of the genera Agrobacterium, Bacillus, Bradyrhizobium, Ervinia, Rhodopseudomonas (RF patent 2322061) is described.

In the prior art, fertilizer based on the bacterial strain Bacillus mucilaginosus - Bac 1208, capable of producing exopolysaccharide that creates useful microflora of the rhizosphere (RF patent 2081867), and fertilizer based on the bacterial strain Bacillus mucilaginosus, capable of transforming poorly soluble phosphorus and potassium compounds into available a form having phytopathogenic activity (RF patent No. 2408722).

Describes the use as a fertilizer (RF patent 2148571) of a dietary supplement consisting of associations of nitrogen-fixing bacteria Azotobacter chroococcum and Beijerinckia fluminensis taken in the ratio (1-0.5) :( 1-0.5) and decomposing phosphorus and potassium-containing bacteria of Bacillus bacteria megaterium and Bacillus mucilaginosus taken in the ratio (1-5: 0.5-2).

The bacterial strain Azotobacter chroococcum BH-1811, used to obtain a biological product designed to increase crop yields and increase their resistance to various diseases, is disclosed in RF patent 2289620.

In the patent of the Russian Federation 2408721 a bacterial strain Agrobacterium radiobacter Ag 1108 is described, which has enhanced nitrogen-fixing properties, and a fertilizer based on it.

The strain of microorganisms Sphingobacterium multivorum proposed for use in the microbiological industry for bacterial fertilizers for tomatoes and cucumbers (RF patent 2458119).

The use of a strain of Pseudomonas brassicacearum for producing fertilizer is described (RF patent 2453596).

It is known to use strains of microorganisms, such as, for example, Serratia plymuthica (WO / 2012.095431), a fungus of the genus Trichoderma (RF patent 2534213), or a composition of Pseudomonas strains mixed with humic fertilizer and Chlorella Vulgaris culture fluid (RF patent 2291620) for biological control plants.

However, the well-known bioadditives used as fertilizers or to protect plants from phytopathogens have several disadvantages due to the insufficiently high efficiency and laboriousness of the method of producing fertilizers and the narrow spectrum of suppression of phytopathogenic microflora. Moreover, most of the known bioadditives used to produce fertilizers do not have antagonistic activity against phytopathogens. The yield increase using known solutions largely depends on the type of soil, the presence of nutrients in it, the thickness of the humus layer and other components.

Thus, the isolation and development of new strains of microorganisms that increase the efficiency of organomineral fertilizers and yield of cultivated plants with antagonistic activity against phytopathogens is an urgent task, the solution of which opens up a fundamental possibility on an industrial scale to achieve high crop yields and high quality obtained crops products while reducing fertilizer applied to the soil, as well as less than the amount or eliminate the use of chemicals to protect plants from pathogens.

Disclosure of invention

The authors of the present invention for the first time proposed a bacterial strain Paenibacillus mucilaginosus Pm 2906 (VKPM number B-12259), which promotes the formation of metabolic products that accelerate the growth of the root and vegetative system of plants and stimulate the production of phytohormones by the plant.

The claimed strain has the ability to dissolve water-insoluble phosphorus and potassium compounds in the soil, and is also actively involved in the nitrification process.

Using the claimed strain as a fertilizer can significantly reduce the amount of nitrogen, phosphorus and potassium fertilizers applied to the soil.

The bacterial strain Paenibacillus mucilaginosus Pm 2906 has an increased ability to synthesize bacteria with a wide range of suppression of phytopathogenic microflora and helps to increase stress resistance, survival and productivity of a wide range of agricultural and ornamental crops.

In one aspect, the invention is directed to a bacterial strain Paenibacillus mucilaginosus Pm 2906 (VKPM B-12259).

In yet another aspect, the invention relates to a method of stimulating growth and protecting plants from diseases.

Another aspect of the invention relates to the use of the bacterial strain Paenibacillus mucilaginosus Pm 2906 as a fertilizer and biological control agent in the prevention or treatment of plant diseases.

The implementation of the invention

The present invention is based on the unexpected discovery that the bacterial strain Paenibacillus mucilaginosus Pm 2906 (VKPM number B-12259) can be used as a highly effective fertilizer that stimulates the growth of the vegetative mass and root system of plants and increases the yield of agricultural and ornamental crops, as well as an agent biological control in the prevention or treatment of plant diseases.

In accordance with the invention, the bacterial strain Paenibacillus mucilaginosus Pm 2906 was isolated from cultivated soils by multi-stage selection and cultivated on Ashby medium (Example 1).

Cultural and morphological characteristics of the bacterial strain Paenibacillus mucilaginosus Pm 2906:

When grown on Ashby agar medium at a temperature of 30 ± 1 ° C for 90-100 hours, the colonies are convex or slightly flattened, colorless or light gray, transparent, mucous, with a smooth edge, viscous consistency.

During microscopy, the cells are fixed, large sticks of regular shape with rounded ends, single or paired spore-forming, with a mucous membrane.

Cell sizes vary with growth on various nutrient media 1-1.4 × 4-7 microns. Gram-negative, Gram stain may vary depending on the stage of development of the culture.

Physiological and biochemical characteristics of the bacterial strain Paenibacillus mucilaginosus Pm 2906 (VKPM B-12259 number):

The strain Paenibacillus mucilaginosus Pm 2906 is grown in a fermenter to a concentration of 120 million spores per 1 ml of solution at pH 7.5-8.5 at a cultivation temperature of 30 ± 5 ° C (Example 1).

As a carbon source, it metabolizes glucose, sucrose, maltose, lactose, arabinose, and hydrolyzes starch. From alcohols assimilates mannitol, glycyrin, lactate.

Ammonium nitrogen, urea and nitrates can be used as a source of nitrogen.

The strain has the ability to dissolve insoluble and poorly soluble compounds of phosphorus and potassium (for example, silicates, apatites, tricalcium phosphate).

The optimum temperature is 31 ± 2 ° C; pH optium - 7.0-7.5; aerobic culture.

Strain conditions for the strain: at a temperature not exceeding + 30 ° C and not lower than -3 ° C, excluding direct sunlight, freezing of the drug is not allowed.

Cultivation conditions - on solid nutrient media at 4-6 ° C.

Antagonistic activity - fungi Streptomyces, Rhizoctonia solani, Sclerotinia, Bipolaris, Fuzarium, as well as powdery mildew and septoria.

In one embodiment of the invention, the fermentation product, which is a suspension of active bacterial cells of the strain Paenibacillus mucilaginosus Pm 2906 (VKPM number) with a titer of at least 120 million spores / ml, is used as fertilizer.

The finished product remains active for 12 months at a temperature of from -3 to + 30 ° C.

The strain Paenibacillus mucilaginosus Pm 2906 according to the invention is used as a fertilizer for cereals (wheat, barley, oats, rye, rice), potatoes, vegetables, berries, flowers, legumes, legumes, conifers, deciduous, ornamental trees and shrubs .

The effectiveness of the claimed strain when used as a fertilizer was investigated in the field on various plant cultures.

In field trials of the biomass of the strain Paenibacillus mucilaginosus Pm 2906 on plum and plum plants using seed treatment at an early stage of the growing season, a significant increase in the volume of the root system and plant height was demonstrated (Example 2).

In field trials of the biomass of the strain Paenibacillus mucilaginosus Pm 2906 on coniferous trees, an increase in the diameter of the root neck and a decrease in the decay of seedlings were noted (Example 3).

In field trials, an increase in potato yield was also noted when using the strain Paenibacillus mucilaginosus Pm 2906 as a fertilizer (Example 4).

In another embodiment, the strain Paenibacillus mucilaginosus Pm 2906 is used as a biological control agent in the prevention or treatment of plant diseases, in particular rhizoctonia (black scab), one of the most harmful and common diseases of potato.

The strain Paenibacillus mucilaginosus Pm 2906 according to the invention has a pronounced inhibitory effect in the open field on pathogens of potato of fungal origin (Example 4).

Preferably, when presowing seed treatment, the consumption rate of the working solution of cells of the bacterial strain Paenibacillus mucilaginosus Pm 2906 at a concentration of 120 million spores / ml per ton of seeds of grain crops (wheat, barley, oats, rye, rice, buckwheat) is 2.0-4.0 l; per ton of leguminous crops - from 1.5 to 5 liters and per ton of potato tubers - from 0.3 to 0.5 liters.

When presowing the soil, the rate of flow of the working solution of cells of the bacteria strain Paenibacillus mucilaginosus Pm 2906 at a concentration of 120 million spores / ml for all crops is preferably 0.4-1.0 l / ha.

When feeding vegetating plants, preferably the flow rate of the working solution is 0.4-1.0 l / ha for cereals, from 0.4 to 1.0 l / ha for cereals and legumes, for sugar beets, rice, corn, sunflower, buckwheat, rape, mustard, potato - from 0.4 to 1.0 l / ha.

When processing fruit, coniferous, deciduous, ornamental trees and shrubs, pre-sowing seed treatment is preferably carried out at the rate of 20-30 ml of cells of the bacterial strain Paenibacillus mucilaginosus Pm 2906 at a concentration of 120 million spores / ml per 200 ml of water, the rate of flow of the working solution is 200 ml per 100 g of seeds, when feeding seedlings and seedlings, it is preferable to water the root solution with a solution of 30-50 ml of cells of the bacterial strain Paenibacillus mucilaginosus Pm 2906 at a concentration of 120 million spores / ml in 5-10 l of water.

The invention is illustrated by the following examples presented to confirm, but not limit the scope of the claims.

Examples

Example 1. The cultivation of the bacterial strain Paenibacillus mucilaginosus Pm 2906 (VKPM number B-12259)

Sterile liquid nutrient medium composition, mass. %: sucrose - 1.0-5.0; K ₂ HPO ₄ - 0.01-0.05; NaCl - 0.01-0.05; MgSO ₄ - 0.01-0.05; NH ₄ NO ₃ - 0.01-0.05; FeSO ₄ - 0.0001-0.0007; CaCO ₃ 0.1-0.8; K ₂ SO ₄ - 0.01-0, corn extract - 0.1-0.5, inoculated with the cell culture Paenibacillus mucilaginosus Pm 2906 dated 02.16.09 from the calculation of 2-10% of the volume of the nutrient medium. Grown for 12-36 hours at a temperature of 30 ± 5 ° C, continuous stirring and aeration equal to 1 volume of air / 1 volume of nutrient medium per minute.

The resulting culture is seeded with a fermenter with pre-sterilized liquid nutrient medium having the composition, mass. %: molasses - 0.5-2.0; KH ₂ PO ₄ 0.01-0.06; MgSO ₄ - 0.005-0.04; NaCl - 0.04-0.08; KNO ₃ - 0.005-0.03; CaCO ₃ - 0.1-0.4, corn extract 0.1-0.4 to 0.5, FeSO4 - 0.0001-0.0007 based on 1-10% of the volume of the nutrient medium. Cultivation is carried out at a temperature of 30 ± 5 ° C, continuous stirring and aeration equal to 1 volume of air / 1 volume of nutrient medium per minute. The cultivation duration is 36-72 hours, pH 7.5-8.5. A slightly viscous liquid culture is obtained containing up to 120 million spores / ml.

The finished fermentation product, which is a suspension of active bacterial cells of the strain Paenibacillus mucilaginosus Pm 2906 (VKPM number) with a titer of at least 120 million spores / ml, is used as a fertilizer and means for the prevention and / or treatment of plants from diseases.

The finished product remains active for 12 months at a temperature of from -3 to + 30 ° C.

The bacterial strain Paenibacillus mucilaginosus Pm 2906, when grown by the indicated method, shows the ability to actively break down water-insoluble compounds of phosphorus and potassium, promotes the development of beneficial microflora of the rhizosphere, and increases the production of plant hormones and plant immunity.

As a result, when applying fertilizer based on the bacterial strain Paenibacillus mucilaginosus Pm 2906, an intensive growth of the root system and vegetative mass of cereals and industrial crops, fruit and coniferous, coniferous, flower-decorative trees and shrubs is observed.

Example 2. Stimulation of the growth of the root system in fruit trees

In 2012, in the Tula region on the territory of the Phytogenetics biotechnology OGP, an experiment was conducted to test fertilizers based on the strain Paenibacillus mucilaginosus Pm 2906. The biomass of the bacterial strain Paenibacillus mucilaginosus Pm 2906 (VKPM number B-12259) at a concentration of at least 120 million spores. ml was obtained under the conditions described in example 1.

Cherry plum and plum plants grown in pots were planted in open ground. A month after planting, they were treated with fertilizer. Processing was carried out 3 times every 2 weeks.

The consumption rate of the working solution (20-30 ml of biomass of the bacterial strain Paenibacillus mucilaginosus Pm 2906 per 200 ml of water) per plant is 25-50 ml.

A month after planting, cherry plum and plum plants were treated by watering with a working solution of fertilizer the root space of the experimental plants.

The method of treatment with fertilizer (watering, spraying or spraying) does not affect the technical result achieved. A person skilled in the art can choose any method of treating plants that is convenient for him, depending on the stage of plant development and climatic conditions.

As a result of fertilizer treatment on the basis of the claimed strain, the volume of the root system increased by 73% in the cherry plum July Rose and by 76% in the plum variety Velichavyaya. The height of the plants treated with fertilizer increased by an average of 64% compared to the control for cherry plum of the July rose variety and by 67% for the Velichavyaya plum variety.

Example 3. Stimulation of survival and development of the root system in conifers

In 2012, an experiment was conducted on the territory of the Shchelkovo training experimental leshoz (Moscow region) to test fertilizer based on the bacterial strain Paenibacillus mucilaginosus Pm 2906. The biomass of the strain Paenibacillus mucilaginosus Pm 2906 at a concentration of at least 200 million cells / ml was obtained under conditions described in example 1.

On the plot of experimental production crops, a single treatment of the root systems of coniferous seedlings with a suspension during planting and small-drop spraying of the aerial parts of plants with a working solution of the preparation were performed.

The consumption rate of the working solution (50 ml of the biomass of the bacterial strain Paenibacillus mucilaginosus Pm 2906 per 10 liters of water) is 2-3 liters of working solution for each plant.

Treatment with a fertilizer based on the bacterial strain Paenibacillus mucilaginosus of European pine seedlings showed an increase in the diameter of the root neck by 50-76% relative to the control and a decrease in the fall of seedlings of ordinary spruce to 2-3%, the drop in seedlings in the control was 14%.

Thus, the use of a fertilizer based on the bacterial strain Paenibacillus mucilaginosus Pm 2906 stimulates the growth of the mass of the root system of plants, increases stress resistance, survival rate and productivity of agricultural and ornamental crops.

Example 4. The increase in productivity

In 2014, in the Krasnodar Territory (Gulkevichsky District, Gulkevichi, CJSC Plemzavod Gulkevichsky), field tests of fertilizers based on the Paenibacillus mucilaginosus strain were carried out.

The biomass of the bacterial strain Paenibacillus mucilaginosus with a titer of at least 120 million spores / ml was obtained under the conditions described in example 1.

A single presowing treatment of winter wheat seeds of the Thunder cultivar was carried out at a rate of bacterial preparation consumption of 3.0 l per 1 ton of seeds, followed by a single spraying of crops at a preparation rate of 0.4-1.0 l / ha.

Sowing of seeds was carried out in experimental plots within 1-15 days from the date of treatment. After 7 (seven) months from the date of sowing of the treated seeds, a single spraying of the crops was carried out.

Seeding time does not affect the achieved technical result; sowing time after treatment can vary from one day to several months, an interval of 1-15 days from the date of processing is only a preferred embodiment of the claimed method.

The time of the subsequent single spraying of winter wheat crops also does not affect the achieved technical result and can vary within wide limits. The person skilled in the art can choose, at their discretion, the appropriate time for subsequent spraying, depending on climatic conditions.

At the end of the growing season, the crop is removed and the total yield is estimated in kg / ha.

The test results are presented in table 1.

The data table. 1 demonstrate that the crops from the seeds treated with a fertilizer based on the Paenibacillus mucilaginosus strain followed by the processing of the crops during the growing season during ripening showed a significant increase in the vegetative mass, and the increase in the yield in the experimental plot relative to the crops from the untreated seeds in the control plot amounted to 11.00%.

Example 5. Increasing productivity and suppressing pathogenic microflora

The biomass of the strain Paenibacillus mucilaginosus at a concentration of not less than 200 million cells / ml was obtained under the conditions described in example 1.

In 2009, in a field experiment on the territory of the scientific and experimental base VNIIKH “Korenevo” of the Lyubertsy district of the Moscow region, an early ripe potato variety (Krepysh) was treated with tubers when planted in sod-podzolic soil, sandy loam, characterized by an acid reaction of the environment and high hydrolytic acidity ( pH KCl = 4.47-4.63; Ng = 4.25-4.52 mEq / 100 g soil); the low amount of absorbed bases and the degree of saturation with them (S = 4.2-4.9 mEq / 100 g of soil; V = 48.5-53.6%); optimal for potato content of mobile phosphorus (213-227 mg / kg of soil) and an average content of exchange potassium (165-192 mg / kg of soil); relatively high humus content (2.39-2.64% humus).

Planting was carried out by clone planter in pre-cut ridges, planting scheme 70 × 30 cm.

The potato yield using the strain-based preparation was 36.3 t / ha at 25 t / ha in the control (the increase relative to the control was 45.2%). Thus, the treatment of potato tubers with a preparation based on the Beijerinckia fluminensis Bf 2806 strain leads to an increase in yield.

The use of the bacterial fertilizer according to the invention had a significant restraining effect on the prevalence and development of fungal diseases on potato tubers, the results of the effect of the drug on potato resistance to fungal diseases are presented in table. 2.

In the conducted experiments, the incidence of potato with rhizoctonia in the tops and tubers was also reduced with a disease prevalence rate of 4.2% and a development rate of 0.7%.

Example A.

In 2013, in a field experiment on the territory of the SEC "Dawn" of the Izobilnensky district of the Stavropol Territory on the variety of peas Pluton. Presowing tillage: cultivation.

The use of the test microbiological preparation in the cultivation of peas of the Pluton variety at a dose of 2.5 l / t (seed treatment) and 0.5 l / ha (spraying the sowing phase of 3 real pea leaves) provided an increase in the yield of pulses in comparison with the control 2.7 c / ha or 13.1%.

In the control variant of the experiment, oppression of vegetative shoots was noted after treatment with seeding with herbicides; this negative effect was not observed when a bacterial preparation was added to the tank mixture.

Phytosanitary monitoring of pea crops in the experimental plot did not reveal damage by root rot of plants in the experimental version. At the same time, bacteriosis was recorded in the control plot on the vegetative organs - 2 points, which was not observed in the experimental plot.

Example B.

In 2010, in a field experiment on the territory of Agrofirm Pobeda LLC, Petrovsky district, Stavropol Territory, on a corn variety hybrid Korn 280. Presowing cultivation of the soil: cultivation.

The use of the test microbiological preparation for cultivating corn on the grain of the Korn 280 hybrid (option No. 1) at a dose of 2.5 l / t (seed treatment) provided an increase in grain yield compared to the control of 5.9 c / ha or 12%.

The use of the test microbiological preparation for cultivating corn on the grain of the Korn 280 hybrid (option No. 2) at a dose of 2.5 l / t (seed treatment) and 0.5 l / ha (spraying the crop in the full germination phase) provided an increase in the grain yield compared with the control of 7.4 kg / ha or 15.2%.

The presence of pitious root rot was observed in all fields of the experiment, but in option 2, where the biological product of the claimed seed strain (spraying) was treated with a biological product, the spread of the disease was reduced to 1%.

Example B. In 2014, in a field experiment on the territory of Agrosoyuz Krasnogvardeisky LLC Krasnogvardeisky district of the Stavropol Territory - winter wheat variety Aksinya.

Presowing tillage: peeling.

The use of the test microbiological preparation of the claimed strain in the cultivation of winter wheat cultivar Aksinya (option No. 1) at a dose of 2.5 l / t (seed treatment) provided an increase in grain yield compared to a control of 9.3 c / ha or 41%.

The use of the test microbiological preparation for the cultivation of winter wheat of the Aksinya variety (option No. 2) at a dose of 2.5 l / t (seed treatment) and 0.5 l / ha (spraying the crop in the tillering phase) provided an increase in grain yield compared with control of 3.8 kg / ha or 17%.

In all fields where drug treatment of the claimed strain took place, there is no visible damage by root rot. A lower degree of damage by fungal plant pathogens is noted:

Septoria - control - 11.8%, in the experiment - 4.3% (1 point);

powdery mildew - in the control 7%, in the experiment - 3.4%.

Example G.

In 2014, in a field experiment on the territory of the Branch of the FSBI “State Sport Commission” the Krasnogvardeisky GSU, the Krasnogvardeisky district of the Stavropol Territory - a variety of winter wheat Brigade.

Presowing tillage: cultivation.

The use of the test drug of the claimed strain in the cultivation of winter wheat of the Brigada variety at a dose of 2.5 l / t (seed treatment) provided an increase in grain yield compared to a control of 4.6 c / ha or 13.5%.

The use of the test drug of the claimed strain at the time of pre-sowing seed treatment significantly increased the resistance of winter wheat to powdery mildew - 3%, and Septoria, and also noted a significant decrease in root rot - in the control plot, the relative indicator was 7%, and in the control - 3%.

The above examples confirm that the Paenibacillus mucilaginosus Pm 2906 strain proposed in accordance with the invention is a highly effective fertilizer that stimulates the growth of the vegetative mass and root system of plants and increases the yield of agricultural and ornamental crops, as well as an anti-phytopathogenic agent in the prevention and / or treatment of plant diseases.

Claims (6)

1. The strain Paenibacillus mucilaginosus, deposited in VKPM under the number B-12259, used as a fertilizer and biological control agent in the prevention or treatment of fungal plant diseases. 2. The use of the strain Paenibacillus mucilaginosus VKPM B-12259 as a fertilizer for crops, fruit, ornamental and coniferous trees and shrubs. 3. The use of strain Paenibacillus mucilaginosus VKPM B-12259 as a biological control agent in the prevention and treatment of fungal plant diseases. 4. The use of claim 3, wherein the disease is rhizoctonia. 5. A method of stimulating the growth and protection of plants from fungal diseases, involving pre-sowing treatment of soil, seeds or crops with microorganism cells, characterized in that a suspension of cell culture of the strain Paenibacillus mucilaginosus VKPM B-12259 at a concentration of at least 120 million spores is used as a biological control agent / ml 6. A method of stimulating the growth and protection of plants from fungal diseases according to claim 5, characterized in that the consumption rate of the working solution per ton of grain seeds is 2.0-4.0 l; per ton of leguminous crops - from 1.5 to 5 liters and per ton of potato tubers - from 0.3 to 0.5 liters.