RU2423702C1 - Method of preparing colour mass for vascular system filling in pathologic examination - Google Patents

Abstract

FIELD: medicine. ^ SUBSTANCE: invention refers to a method of preparing a colour mass for vascular system filling in pathologic examinations which involves premixing a dye - a water-soluble acrylic colour and water in the ratio 0.9-1.1 portions to 0.18-0.21 portions to form a homogeneous mass, adding 9.7-9.9 portions of water and 0.18-0.21 portions of 96 % alcohol. It is followed with retempering and adding 9.7-9.9 portions of 96% alcohol, keeping for 12 hours and filtering. ^ EFFECT: improved colour mass for vascular system filling and simplified manufacturing procedure. ^ 1 dwg, 1 ex

Description

The invention relates to the field of veterinary medicine, medicine, biology and can be used for the introduction (filling) of colored masses into the vascular system.

To differentiate blood vessels from other anatomical formations (for example, lymphatic vessels), it is necessary to use special injection formulations (paints).

The analogs were taken: the Teichmann mass and the mass containing chalk, gypsum, flour and cinnabar (for simplicity, we denote its mass No. 1).

Teichmann mass:

sifted chalk clean

cinnabar

natural drying oil.

Grind chalk with cinnabar, adding drying oil for 2-3 hours, roll the resulting homogeneous mass into balls and store in jars under water, which, if necessary, are kneaded and ground in gasoline or ether (Yaroslavtsev B.M. Anatomical technique. Frunze, 1961, S.296).

The disadvantage is that although the mass is simple in composition, it penetrates perfectly into small vessels, but it is very difficult to prepare, and this process takes a long time.

In addition, drying oil is a film-forming substance based on vegetable oils and, in chemical properties, is the opposite of the composition of blood and lymph. Working with gasoline or ether is dangerous for the researcher.

Known mass for filling vessels, containing:

a piece of chalk

gypsum

wheat flour

cinnabar

Mix all ingredients, grind in a mortar, dilute with 5% formalin solution (Yaroslavtsev B.M. Anatomical technique. Frunze, 1961, p.297. Big medical encyclopedia. Volume 14. Blood vessels. P.265. Http: // lib. e-science.ru/book/79/page/265.html).

The disadvantage is that, although the mass is simple to perform, it perfectly pours the smallest vessels, hardens well, but breaks, which can lead to the formation of artifacts or even reduce the researcher's work to zero.

The prototype of the invention is the resinous mass.

Resinous mass:

rectified alcohol

rosin

Wheat flour

paint

Grind the rosin in alcohol, add flour, mix well and add paint (Yaroslavtsev B.M. Anatomical technique. Frunze, 1961, P.296).

The disadvantage of the resinous mass is that the mass has a very thick consistency and adheres quite strongly to the wall of the syringe, which greatly complicates the work of the researcher, in addition, its preparation takes up to 10 days.

The technical essence of the present invention is to improve the quality of the colored mass for filling the vascular system and simplifying its preparation.

Therefore, it must meet certain conditions: 1. be "identical" or close in chemical composition of blood; 2. ductile and fill even the smallest vessels; 3. maintain elasticity in the vessels, i.e. Do not break or crumble; 4. be resistant, including during storage, fixation and wiring of the material in various liquids; 5. Do not diffuse through the vessel wall; 6. be affordable and easy to prepare, use, store; 7. not have a harmful effect on the health of the researcher

The specified technical essence is achieved by the fact that in the method of preparing a colored mass for pouring the vascular system during anatomical studies, including mixing paint with a water-alcohol solution, water-soluble acrylic paint is used as a dye, which is pre-mixed with water in a ratio of 0.9-1, 1 part to 0.18-0.21 parts to form a homogeneous mass, add 9.7-9.9 parts of water and 0.18-0.21 parts of 96% alcohol to it, re-mix, then add 9.7 -9.9 parts of 96% alcohol, incubated for 12 hours and filtered.

The proposed color mass for filling the vascular system - the mass consists of:

Example 1. Red mass - for filling the arterial system: acrylic red - 4.9, alcohol 96% - 49.0, flowing water - 49.0;

Example 2. The blue mass is for the venous system: acrylic blue - 5.1, alcohol 96% - 51.0, flowing water - 51.0.

The choice of the composition of the prepared paint for the TMK mass was determined by the fact that acrylic (polymethyl acrylate) is a thermoplastic polymer material that is produced by polymerization of 100% pure raw materials (monomers). It is a pure and transparent material with the following characteristics: good mechanical properties; suitable for heat treatment; light weight combined with high strength; resistant to ultraviolet radiation.

The pigment particles of acrylic paints are interconnected by a liquid plastic mass resembling milk, which, after drying, becomes transparent. Acrylic is soluble in water, which can be used as a thinner and remover for cleaning brushes and palettes. Unlike other water-based paints, acrylic becomes insoluble after drying. This is his positive quality, since the finished work is no longer subject to the influence of water (Acrylic paints. Http://www.artservice.ru/eng/catalog/acril.htm).

Acrylic dries very quickly - this is its advantage over other paints. Acrylic can be applied both in a very liquid diluted state (diluted with water) and in a pasty state (using special thickeners), while acrylic does not form cracks, unlike oil paints. Drying of acrylic paints occurs as a result of evaporation of water; wherein the paint forms a chemically stable elastic film consisting of a pigment and a binder. Unlike oil paints, dried acrylic is characterized by greater strength (less brittle); the paint does not crumble, its layer does not form cracks, the surface does not peel off.

This quality is necessary for filling and further preparation. All acrylic paints are based on acrylic acid and are synthetic. They are distinguished by high luminosity, water and heat resistance and tight adhesion to the surface. Such paints are versatile and easy to handle.

The paint lays down with an even film, glistens a little, does not require fixing with fasteners and varnishes, has the ability to form a film that can be washed off after drying only with special solvents that are not used in histological technique. Over time, acrylic paint does not wrinkle, is not susceptible to the effects of normal temperature extremes and changes in humidity conditions, which is important for micro-research and archiving of drugs (Acrylic paints. Http://www.plazmastroy.ru/).

Acrylic paints and varnishes can be used on any non-greasy basis such as glass, wood, metal, canvas, canvas and the like. But the use of acrylics in the anatomical technique is not mentioned in the literature available to us. Fresh acrylic paint is removed from objects with water, but when dried requires special solvents. The paint itself is odorless, opaque, creamy consistency.

To prepare the colored mass, you must:

1. Put in a porcelain dish 5.1 g of acrylic.

2. Pour 1.1 ml of water into the acrylic with flowing cool water and mix thoroughly in a circular motion until a homogeneous mass is formed.

3. Dilute 49.9 ml of water with 1.1 ml of 96 ° alcohol. The resulting liquid of 5.1 ml in circular motions is added to a homogeneous mass of acrylic and water, mixing thoroughly until a homogeneous mass is formed.

4. Add the remaining alcohol (49.9 ml) to the resulting mass, mixing thoroughly until a homogeneous mass is formed.

5. Leave the resulting mass for 12 hours.

6. Filter three times through filter paper.

7. The mass of TMK can be stored both in glass and in plastic containers with a ground or well-twisted lid at room temperature. Shelf-life Unlimited.

If it is not important to differentiate arterial and venous vessels, but it is only necessary to distinguish blood vessels from other anatomical formations (for example, the lymphatic system), then instead of blue and red acrylic, you can take white. In this case, the TMK white mass recipe looks like this: white acrylic - 5.2; alcohol 96% - 48.0; running water - 48.0

The preparation of the mass is identical for filling venous or arterial systems.

The drawing shows the color mass for filling the vascular system.

In photo A - large and small blood vessels of the lung parenchyma, well filled with white mass.

On a photo In - Vessels before introduction of red mass.

In the photo C - After the introduction of the red mass.

Pictured D - Fixed lung tissue in 10% formalin. 1 - Arteries filled with red mass, 2 - Veins - blue mass.

In the photo, E - A lung injected with a mass of Gerot - 3, in the arteries a red mass - 4.

In the photo F - Red mass - 5, blood cells - 6.

In the photo G - Blue mass, which does not diffuse beyond the walls - 7.

Colored mass for filling the vascular system is acceptable for purely macroscopic studies (preparation) of blood vessels, for this it is only necessary to increase the dose of acrylic, which hardens well.

The use of colored mass for filling the vascular system:

1. It is necessary to take fresh material, which is placed in warm water.

2. The colored mass is introduced both into empty vessels and into blood vessels, since acrylic is a water-soluble paint, therefore:

- the mass penetrates well into both large and small blood vessels (see drawing - A, B, C). The drawing shows large pulmonary vessels before the introduction and after the red mass of TMK. In addition, the mass of TMK contrasts well with the surrounding tissue, which is a factor in visual control (see drawing - A). In drawing A - large and small vessels of the lung into which the white mass was introduced, it contrasts well with the surrounding tissue;

- the mass can be used for histological studies (see drawing - D), as well as in conjunction with other masses, for example Gerot. In this case, the mass under the influence of 10% formalin does not go beyond the vessels and does not contact with other paints (Gerot). In addition, due to its chemical properties it hardens well (see drawing - D, E), which allows it to fully occupy the entire lumen of the vessel, this can also be used for macroscopic studies;

- the mass when connected to blood does not coagulate, but when combined, forms a homogeneous mass (due to the water-soluble base), while it does not diffuse beyond the wall (see drawing - G), which allows you to accurately differentiate blood vessels from other formations.

3. After the introduction of the mass, it is necessary to make a light tissue massage to completely fill the vessel. When the mass enters the surface of the organ, tissue impregnation does not occur.

Further, the material is used in accordance with the objectives of the research. In our studies, material with a poured vascular system was recorded according to generally accepted methods (Korzhevsky D.E. Summary of histological techniques for doctors and laboratory assistants-histologists. - Publishing House LLC Krof. - St. Petersburg. - 2005. P.46). The mass of TMK can be used in conjunction with other masses, for example Gerot (see drawing - E, 3, 4).

Claims (1)

A method of preparing a colored mass for filling the vascular system during anatomical studies, including mixing paint with a water-alcohol solution, characterized in that a water-soluble acrylic paint is used as a dye, which is pre-mixed with water in a ratio of 0.9-1.1 part to 0 , 18-0.21 parts to form a homogeneous mass, add 9.7-9.9 parts of water and 0.18-0.21 parts of 96% alcohol to it, mix again, after which 9.7-9.9 are added parts of 96% alcohol, incubated for 12 hours and filtered.

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