RU2384335C1 - Agent for colibacteriosis treatment in young stock of farm animals - Google Patents

Abstract

FIELD: veterinary science.

SUBSTANCE: preparation contains the following components, wt %: Norfloxacin 1-3, Dioxidin 1-3, Sodium chloride 10, Potassium chloride 0.4, Low-molecular polyvinyl pyrrolidone 49.6, Glucose - the rest.

EFFECT: agent application allows increasing efficiency of colibacteriosis treatment in young stock of farm animals.

10 tbl, 2 ex

Description

The invention relates to veterinary medicine.

The success of treatment for infectious diseases of animals of bacterial etiology largely depends on the correct choice of drugs, their timely use. One of the main means of combating infectious agents is antibacterial drugs, the use of which in medical and preventive work not only does not weaken, but is also constantly expanding.

However, due to their widespread and often uncontrolled use, primarily antibiotics, the problem of resistance of microorganisms to them arose. In addition, many antibacterial agents in some cases have a negative effect on the body.

To overcome the drug resistance of bacteria, an urgent problem is the development and implementation of complex antibacterial drugs in veterinary practice. The high therapeutic efficacy of such drugs is due to their multicomponent, synergistic effect and lower toxicity.

At the same time, many researchers consider sharp circulatory disturbances arising from the loss of fluid and electrolytes by the body as the main cause of death of a newborn youngster during acute digestive disorders. The use of rehydration therapy contributes to the normalization of water-salt metabolism, acid-base balance, the restoration of osmotic pressure and blood volume. In glucose-salt solution, it is necessary to include drugs that have a detrimental effect on the gram-negative microflora, taking into account the sensitivity to them, as well as solubility in water.

Known analogue - the drug "Diarin" for the treatment and prevention of gastrointestinal diseases of young animals, which contains, wt.%: Dioxidine 0.6-0.9; 40% lactic acid 10.0-15.0; methyluracil 0.6-0.9; boiled water - the rest [RU 2105548 C1, 02.27.1998].

A known tool for the treatment of gastrointestinal diseases of young farm animals "Diacetin" containing antibacterial dioxidine, anti-inflammatory and antipyretic agent - acetylsalicylic acid, immunomodulating agent thymalin and natural zeolites in the following ratio, wt.%: Dioxidine - 2.5-5, 0; acetylsalicylic acid - 25.0-75.0; thymalin - 0.5-0.75; zeolites - up to 100 [RU 2075972 C1, 03/27/1997].

The prototype of the invention is the preparation Enromag, which is an aqueous solution of enrofloxacin. The disadvantage of the drug is its low efficiency due to the fact that the enromag is a single drug containing only one active substance [Yakovlev S.V. The place of fluoroquinolones in the treatment of bacterial infections // Antibiotics and chemotherapy. - 1999.- t.44. - No. 7. - S. 38-44].

The technical result is an increase in the effectiveness of the treatment of colibacillosis in young farm animals.

The technical result is achieved by using the drug for the treatment of colibacillosis in young farm animals, consisting of the following components, wt.%:

Norfloxacin - 1-3,

Dioxidine - 1-3,

Sodium chloride - 10,

Potassium chloride - 0.4,

Low molecular weight polyvinylpyrrolidone - 49.6,

Glucose is the rest.

Norfloxacin - (formula C ₁₆ H ₁₈ FN ₃ O ₃ ) is a drug that is similar in structure to pefloxacin. It differs from pefloxacin in the absence of a methyl group in the piperazinyl nucleus. It is a metabolite of pefloxacin, with the use of which a significant amount of norfloxacin is excreted in the urine [Mashkovsky M.D. Medicines In two parts. Part 2. - Ed. reslave. and add. - M .: Medicine, 1993. - S.353].

Dioxidine - a quinoxaline drug - 1,4-di-N-oxide-2,3-di (oxymethyl) quinoxaline [Chervyakov D.K. et al. Medicines in veterinary medicine. - M .: Kolos, 1977, p. 215]. The antibacterial action of dioxidine is based on the selective suppression of DNA synthesis. In addition, the drug reduces the activity of extracellular nuclease and plasmocoagulase in microorganisms, which leads to dysfunction of the genetic apparatus of the microbial cell, which suppresses the biosynthesis of biologically active macromolecules, which are pathogenicity factors.

Sodium chloride - NaCl - is a cubic crystal or a white crystalline powder, odorless, salty taste. Soluble in water (1: 3). The solutions are sterilized by fluid steam at a temperature of + 100 ° C for 30 minutes or in an autoclave at a temperature of + 120 ° C for 15-20 minutes [Mashkovsky M.D. Medicines In two parts. Part II. - 12th ed. reslave. and add. - M .: Medicine, 1994. - S.131-132].

Potassium chloride - KCl - colorless crystals or odorless white crystalline powder; tastes salty. Soluble in water (1: 3), practically insoluble in alcohol. Potassium is the main intracellular ion, just like the main extracellular ion is sodium [Mashkovsky M. D. Medicines In two parts. Part II. - 12th ed. reslave. and add. - M .: Medicine, 1994. - S.143-144].

Glucose - chemical formula

chemical formula

It is a colorless crystal or odorless white crystalline powder, sweet in taste. Soluble in water (1: 1.5), sparingly soluble in alcohol. The solutions are sterilized at a temperature of + 100 ° C for 60 minutes or at a temperature of +119, + 121 ° C for 5-7 minutes: the pH of the solutions is 3.0-4.0 [Mashkovsky M.D. Medicines In two parts. Part II. - 12th ed. reslave. and add. - M .: Medicine, 1994. - S.219-220].

Low molecular weight polyvinylpyrrolidone - chemical formula

It is a white powder. It is very soluble in water and mineral acids, soluble in alcohol, glycerin, acetone, dimethylformamide and products of chlorination of hydrocarbons, insoluble in ether and aliphatic hydrocarbons [Polydec-Fabini R., Beirich T. Organic analysis: Trans. with him. - L .: Chemistry, 1981. - S.528-529].

Sodium and potassium chlorides normalize the water-salt balance. Glucose is a source of easily digestible energy material by the body, which increases the body's resistance and improves its function. Low molecular weight polyvinylpyrrolidone helps to remove toxins from the gastrointestinal tract and the body as a whole.

The composition of the prototype Enromag and the drug for the treatment of colibacteriosis in young farm animals as an active substance includes drugs of one pharmacological group - fluoroquinolones - substances that have no analogues in the natural environment. At the same time, Enromag is a single drug, and in the drug for the treatment of colibacteriosis in young farm animals, the combined use of norfloxacin and dioxidine takes place, which provides an increase in the effectiveness of the treatment of colibacteriosis, a quick recovery of sick animals, a decrease in the consumption of active substances and their side effects on the body.

The finished product consists of two packages. Package No. 1 contains the active ingredients: norfloxacin, dioxidine, sodium chloride, potassium chloride, glucose. Package No. 2 contains a filler: polyvinylpyrrolidone low molecular weight. The preparation is made as follows. Glucose, sodium chloride, potassium chloride, dioxidine and norfloxacin are successively introduced into the mixer. Mixing of the components, subsequently packaged in bags No. 1, is carried out for 1 hour until the components are evenly distributed. Polyvinylpyrrolidone is packaged in bag No. 2.

A drug is used to treat colibacteriosis in young farm animals as follows. The contents of packages No. 1 and No. 2 are dissolved in 1 liter of warm (37-38 ° C) drinking water. The treatment solution is prepared immediately before use and immediately used. The solution is applied individually orally at a dose of 10 ml / kg body weight daily 2 times a day with an interval of 12 hours for 3 days. In severe cases of the disease on the first day, the drug is drunk in a double dose.

The essence of the method is illustrated by examples.

Example 1. The study of the optimal ratio of components

1.1 the study of the optimal ratio of norfloxacin and dioxidine in experiments in vitro

The optimal ratio of norfloxacin and dioxidine in the preparation was studied in vitro by the method of serial dilutions in meat and peptone broth (MPB) with determination of the minimum bacteriostatic concentration (MBsK). Comparison of the antimicrobial activity of norfloxacin and dioxidine and combinations of norfloxacin and dioxidine in ratios of 2: 1; 2: 2; 1: 2 was carried out in relation to Salmonella - Salmonella cholerae suis, E. coli K 88 (p) and E. coli K 99 (p), typified by morphological, biochemical, cultural and serological properties.

It was established (Table 1) that the combination of norfloxacin and dioxidine in a ratio of 2: 2 had the most pronounced antimicrobial effect in relation to the studied strains of microorganisms.

Table 1 Antimicrobial activity of combinations of norfloxacin and dioxidine Type of microorganisms Minimum bacteriostatic concentration, (MBsK), mcg / ml one 2 3 four 5 S. cholerae suis 0.24 15.6 0.98 / 0.001 0.49 / 0.02 1.90 / 0.02 E.coli K 88 (p) 0.24 31,2 3.90 / 0.04 1.95 / 0.08 7.80 / 0.08 E.coli K 99 (p) 0.49 15.6 3.90 / 0.04 1.95 / 0.08 3.90 / 0.04 Designations in the table: 1 - norfloxacin 2 - dioxidine 3 - a combination of norfloxacin and dioxidine in a ratio of 2: 1 4 - a combination of norfloxacin and dioxidine in a ratio of 2: 2 5 - a combination of norfloxacin and dioxidine in a ratio of 1: 2 Note. In the numerator - MBsK of the drug as a whole; in the denominator - MBsK for Far East

The MBSK of this combination with respect to Salmonella cholerae suis was 0.49 μg / ml, and with respect to E.coli K 88 (p) and E.coli K 99 (p) it was 1.95 μg / ml and was superior to MBSK as components of the combination - norfloxacin and dioxidine, and combinations of norfloxacin and dioxidine in a ratio of 2: 1 and 1: 2.

The fractional inhibitory concentration (FIC) of norfloxacin in the combination of norfloxacin and dioxidine in a 2: 2 ratio in the experiment with Salmonella cholerae suis was 0.040, and dioxidine was 0.001.

The FIC index (the sum of the fractional inhibitory concentrations of the components) of the combination of norfloxacin and dioxidine in an optimal ratio of 2: 2 in an experiment with a culture of Salmonella cholerae suis was 0.041.

The FIC of norfloxacin in combination with the optimal ratio of norfloxacin and dioxidine (2: 2) in the experiment with the culture of E. coli K 88 (p) was 0.158, dioxidine - 0.001.

The FIC index of the combination of norfloxacin and dioxidine in an optimal ratio of 2: 2 in an experiment with a culture of E. coli K 88 (p) was 0.159.

The FIC of norfloxacin in combination with the optimal ratio of norfloxacin and dioxidine (2: 2) in the experiment with the culture of E. coli K 99 (p) was 0.079, dioxidine - 0.002.

The FIC index of the combination of norfloxacin and dioxidine in an optimal ratio of 2: 2 in an experiment with a culture of E. coli K 99 (p) was 0.081.

The fractional inhibitory concentration (FIC) of norfloxacin in the combination of norfloxacin and dioxidine in the ratio of 2: 1 and 1: 2 in the experiment with Salmonella cholerae suis was respectively 0.079, dioxidine - 0.001 and 0.002.

The FIC index (the sum of the fractional inhibitory concentrations of the components) of the combination of norfloxacin and dioxidine in the ratio of 2: 1 and 1: 2 in the experiment with the Salmonella cholerae suis culture was 0.080 and 0.081, respectively.

The FIC of norfloxacin in the combination of norfloxacin and dioxidine in the ratio of 2: 1 and 1: 2 in the experiment with the culture of E. coli K 88 (p) was 0.325, dioxidine - 0.001 and 0.005.

The FIC index of the combination of norfloxacin and dioxidine in the ratio of 2: 1 and 1: 2 in the experiment with the E. coli K 88 (p) culture was 0.326 and 0.330, respectively.

The FIC of norfloxacin in the combination of norfloxacin and dioxidine in the ratio of 2: 1 and 1: 2 in the experiment with the culture of E. coli K 99 (p) was 0.163 and 0.081, respectively, and dioxidine 0.003 and 0.005.

The FIC index of the combination of norfloxacin and dioxidine in the ratio of 2: 1 and 1: 2 in the experiment with the culture of E. coli K 99 (p) was 0.166 and 0.086, respectively.

Thus, it turned out that, compared with the combination of norfloxacin and dioxidine in the ratio of 2: 2, the combination of norfloxacin and dioxidine in the ratio of 2: 1 and 1: 2 in relation to the studied cultures is 2 times less effective.

1.2 the study of the optimal ratio of norfloxacin and dioxidine in experiments in vivo

The optimal ratio of norfloxacin and dioxidine was studied in experiments on the treatment of colibacteriosis in calves.

The diagnosis of colibacteriosis was established on the basis of data from a clinical examination of animals, autopsy, laboratory tests taking into account the epizootic situation on the farm. In the experiment on calves in the treatment of colibacteriosis, the higher efficiency of the combination of norfloxacin and dioxidine in a ratio of 2: 2 was confirmed.

The research results are presented in table 2.

table 2 The effectiveness of the drug for the treatment of colibacillosis in young farm animals with different ratios of norfloxacin and dioxidine in the treatment of colibacillosis in calves Indicators The ratio of norfloxacin and dioxidine one 2 3 Number of animals, goal. fourteen fifteen fourteen Duration of treatment, days 3.2 ± 0.3 2.9 ± 0.4 3.6 ± 0.5 Recover, goal. 13 fourteen 12 % 92.9 93.3 85.7 Remained sick, goal. one one 2 % 7.1 6.7 14.3 It’s a goal. - - - % - - - Therapeutic efficacy,% 92.9 93.3 85.7 Designations in the table: 1 - a combination of norfloxacin and dioxidine in a ratio of 2: 1 2 - a combination of norfloxacin and dioxidine in a ratio of 2: 2 3 - a combination of norfloxacin and dioxidine in a ratio of 1: 2

1.3 Antimicrobial activity

The study of antimicrobial activity was carried out by the method of serial dilutions. As test cultures, reference and field (pathogenic) strains of microorganisms, the causative agents of gastrointestinal diseases of piglets and calves, typified by morphological, tinctorial, cultural, biochemical and serological properties were used. As a liquid nutrient medium, meat-peptone broth was used.

The main solution of the drug is 1000 μg / ml. The drug consisted of the following components, wt.%:

Norfloxacin - 2,

Dioxidine - 2,

Sodium chloride - 10,

Potassium chloride - 0.4,

Glucose - 36,

Polyvinylpyrrolidone low molecular weight - 49.6.

A working solution was prepared from the main solution of the preparation. 4.5 ml of meat-peptone broth were poured into 16 sterile tubes. 4.5 ml of the preparation was added to the first tube, mixed, after which 4.5 ml of the resulting solution was transferred to the next tube, thus continuing to dilute to 15 tubes. As a control of culture growth, a 16 test tube containing no preparation was used.

The content of microbial cells in 1 ml of medium was 500 thousand.

Crops were incubated at 37 ° C for 18-20 hours. After the incubation period, the result was taken into account.

The minimum bacteriostatic concentration (MBsK) of the drug for the treatment of colibacteriosis in young farm animals was determined by the method of serial dilutions in meat and peptone broth (MPB); minimum bactericidal concentration - by seeding from tubes with a transparent medium on solid nutrient media (MPA) that do not contain the drug.

The results of the studies are presented in table 3.

Table 3 Antimicrobial activity of the drug for the treatment of colibacillosis in young farm animals (μg / ml) Microorganism culture MBSK MBCC Salmonella cholerae suis 0.48 0.96 S.dublin 0.48 0.96 S.thyphimurium 0.96 1.95 Salmonella cholerae suis (n) 0.96 1.95 S.dublin (n) 0.96 1.95 E.coli 08 0.96 1.95 E.coli A 20 1.95 3.9 E.coli 0142 (p) 0.96 1.95 E.coli K 88 (p) 1.95 3.9 E.coli K 99 (p) 1.95 7.8 Pseudomonas aeruginosae 15.6 31.2

From table 3 it is seen that the drug has a wide spectrum of antimicrobial activity in relation to the tested cultures of microorganisms. The minimum bacteriostatic concentration of the drug in relation to gram-negative microorganisms was 0.48-15.6 μg / ml.

For museum strains of Salmonella, the minimum bacteriostatic concentration of the drug was 0.48-0.96 μg / ml, field strains - 0.96 μg / ml. In relation to museum and field strains of E. coli, the minimum bacteriostatic concentration of the drug was 0.96-1.95 μg / ml.

Pseudomonas aeruginosa turned out to be the most resistant to the drug; a concentration of 15.6 μg / ml was necessary to delay its growth.

Bactericidal properties of the drug were manifested in concentrations twice as high as bacteriostatic.

The results of the studies showed that the drug has a pronounced antimicrobial effect.

Example 2. Clinical trials of a drug for the treatment of colibacteriosis in young farm animals

2.1. The effectiveness of the treatment of colibacillosis in piglets

The study of the effectiveness of treatment of colibacillosis in piglets was carried out in three experiments on pigs of 7-10 days of age weighing 2.5-3.7 kg.

According to the principle of paired analogues, animals were divided into two groups.

The diagnosis of colibacteriosis was established comprehensively on the basis of data from a clinical examination of animals, laboratory tests, postmortem autopsy, taking into account the epizootic situation on the farm.

The animals were monitored daily for 10-15 days, taking into account the general condition, mortality, growth rate, and timing of recovery.

The first experiment was conducted in the SCA "Homeland" of the Rossoshansky district of the Voronezh region from February to March 2005.

For the treatment of piglets in the control group (55 goals), enromag-o was used at a dose of 0.5 ml / 10 kg of body weight orally in a mixture with warm water for 3 days. The only source of water during the treatment was the treatment mixture.

For the treatment of piglets of the experimental group (60 goals), a drug was used to treat colibacteriosis in young farm animals in the following ratio of components, wt.%:

Norfloxacin - 2,

Dioxidine - 2,

Sodium chloride - 10,

Potassium chloride - 0.4,

Glucose - 36,

Polyvinylpyrrolidone low molecular weight - 49.6.

Before use, the drug was dissolved in warm (37-38 ° C) drinking water at the rate of 50 g of the drug per 1 liter of water. Piglets were prescribed a drug for the treatment of colibacillosis in young farm animals orally at a dose of 10 ml / kg body weight daily for 3 days. The results of the experiment to study the effectiveness of the treatment of colibacteriosis in piglets are presented in table 4.

Table 4 The effectiveness of the treatment of colibacillosis in piglets Indicators Group of animals Control Experienced Number of animals, goal. 55 60 Recover, goal. 47 53 % 85.5 88.3 Remained sick, goal. 3 3 % 5,4 5,0 It’s a goal. 5 four % 9.1 6.7 Duration of recovery, days 4.0 ± 0.4 3.9 ± 0.5

The second experiment was conducted at the Aleksandrovskaya Artists' Union, Rossoshansky District, Voronezh Region from February to March 2005.

For the treatment of piglets in the control group (54 animals), enromag-o was used at a dose of 0.5 ml / 10 kg of body weight orally in a mixture with warm water for 3 days. The only source of water during the treatment was the treatment mixture.

For the treatment of piglets from the experimental group (58 goals), a drug was used to treat colibacteriosis in young farm animals in the following ratio of components, wt.%:

Norfloxacin - 2,

Dioxidine - 2,

Sodium chloride - 10,

Potassium chloride - 0.4,

Glucose - 36,

Polyvinylpyrrolidone low molecular weight - 49.6.

Before use, the drug was dissolved in warm (37-38 ° C) drinking water at the rate of 50 g of the drug per 1 liter of water. Piglets were prescribed the drug orally at a dose of 10 ml / kg body weight daily for 3 days. The results of an experiment to study the effectiveness of the treatment of colibacteriosis in piglets are presented in table 5.

Table 5 The effectiveness of the treatment of colibacillosis in piglets Indicators Group of animals Control Experienced Number of animals, goal. 54 58 Recover, goal. 46 51 % 85,2 87.9 Remained sick, goal. 5 5 % 9.3 8.6 It’s a goal. 3 2 % 5.5 3,5 Duration of recovery, days 4.0 ± 0.5 3.8 ± 0.5

The third experiment was conducted at the Dokuchaev Agricultural Association in the Talovsk District of the Voronezh Region from September to November 2005.

For the treatment of piglets in the control group (50 animals), enromag-o was used at a dose of 0.5 ml / 10 kg of body weight orally in a mixture with warm water for 3 days. The only source of water during the treatment was the treatment mixture.

For the treatment of piglets of the experimental group (56 goals), a drug was used to treat colibacteriosis in young farm animals with the following ratio of components wt.%:

Norfloxacin - 2,

Dioxidine - 2,

Sodium chloride - 10,

Potassium chloride - 0.4,

Glucose - 36,

Polyvinylpyrrolidone low molecular weight - 49.6.

Before use, the drug was dissolved in warm (37-38 ° C) drinking water at the rate of 50 g of the drug per 1 liter of water. Piglets were prescribed the drug orally at a dose of 10 ml / kg body weight daily for 3 days. The results of the experiment to study the effectiveness of the treatment of colibacteriosis in piglets are presented in table 6.

Table 6 The effectiveness of the treatment of colibacillosis in piglets Indicators Group of animals Control Experienced Number of animals, goal. fifty 56 Recover, goal. 43 fifty % 86.0 89.3 Remained sick, goal. 5 four % 10.0 7.1 It’s a goal. 2 2 % 4.0 3.6 Duration of recovery, days 3.7 ± 0.3 3.2 ± 0.4

As studies have shown, the drug for the treatment of colibacillosis in young farm animals has high therapeutic efficacy in the treatment of colibacillosis in piglets. In all experiments, when using the drug for the treatment of colibacteriosis in young farm animals, the recovery time and mortality of animals decreased compared with the control groups.

2.2. The effectiveness of the treatment of colibacillosis in calves

The study of the effectiveness of the treatment of colibacillosis in calves was carried out in four experiments on calves of 7-10 days of age weighing 35-45 kg.

According to the principle of paired analogues, animals were divided into two groups.

The diagnosis of colibacteriosis was established comprehensively on the basis of data from a clinical examination of animals, laboratory tests, postmortem autopsy, taking into account the epizootic situation on the farm.

The animals were monitored daily for 10-15 days, taking into account the general condition, mortality, growth rate, and timing of recovery.

The first experiment was conducted at OAO Yubileinoye, Khokholsky District, Voronezh Region, from September to November 2004.

For the treatment of calves in the control group (18 goals), enromag-o was used at a dose of 0.5 ml / 10 kg of body weight orally in a mixture with warm water for 3 days. The only source of water during the treatment was the treatment mixture.

For the treatment of calves of the experimental group (20 goals), a drug was used to treat colibacteriosis in young farm animals with the following ratio of components wt.%:

Norfloxacin - 2,

Dioxidine - 2,

Sodium chloride - 10,

Potassium chloride - 0.4,

Glucose - 36,

Polyvinylpyrrolidone low molecular weight - 49.6.

Before use, the drug was dissolved in warm (37-38 ° C) drinking water at the rate of 50 g of the drug per 1 liter of water. The calves were prescribed the drug orally at a dose of 10 ml / kg body weight daily for 3 days. The results of the experiment to study the effectiveness of treatment of colibacteriosis in calves are presented in table 7.

Table 7 The effectiveness of the treatment of colibacillosis in calves Indicators Group of animals Control Experienced Number of animals, goal. eighteen twenty Recover, goal. 16 eighteen % 88.9 90.0 Remained sick, goal. one 2 % 5,6 10.0 It’s a goal. one - % 5,6 - Duration of recovery, days 3.0 ± 0.5 2.8 ± 0.5

The second experiment was conducted in the SHA "Freedom" of the Rossoshansky district of the Voronezh region from February to March 2005.

For the treatment of calves in the control group (37 goals), enromag-o was used at a dose of 0.5 ml / 10 kg of body weight orally in a mixture with warm water for 3 days. The only source of water during the treatment was the treatment mixture.

For the treatment of calves of the experimental group (41 goals), a drug was used to treat colibacteriosis in young farm animals in the following ratio of components, wt.%:

Norfloxacin - 2,

Dioxidine - 2,

Sodium chloride - 10,

Potassium chloride - 0.4,

Glucose - 36,

Polyvinylpyrrolidone low molecular weight - 49.6.

Before use, the drug was dissolved in warm (37-38 ° C) drinking water at the rate of 50 g of the drug per 1 liter of water. The calves were prescribed the drug orally at a dose of 10 ml / kg body weight daily for 3 days. The results of the experiment to study the effectiveness of treatment of colibacteriosis in calves are presented in table 8.

Table 8 The effectiveness of the treatment of colibacillosis in calves Indicators Group of animals Control Experienced Number of animals, goal. 37 41 Recover, goal. 33 37 % 89.2 90.2 Remained sick, goal. 3 3 % 8.1 7.3 It’s a goal. one one % 2.7 2.5 Duration of recovery, days 4.8 ± 0.5 4,5 ± 0,5

The third experiment was conducted at Krinichenskoye LLC in the Ostrogozhsky District of the Voronezh Region from January to March 2005.

For the treatment of calves in the control group (43 animals), enromag-o was used at a dose of 0.5 ml / 10 kg of body weight orally in a mixture with warm water for 3 days. The only source of water during the treatment was the treatment mixture.

For the treatment of calves of the experimental group (48 goals), the drug was used to treat colibacteriosis in young farm animals in the following ratio of components, wt.%:

Norfloxacin - 2,

Dioxidine - 2,

Sodium chloride - 10,

Potassium chloride - 0.4,

Glucose - 36,

Polyvinylpyrrolidone low molecular weight - 49.6.

Before use, the drug was dissolved in warm (37-38 ° C) drinking water at the rate of 50 g of the drug per 1 liter of water. The calves were prescribed the drug orally at a dose of 10 ml / kg body weight daily for 3 days. The results of the experiment to study the effectiveness of treatment of colibacteriosis in calves are presented in table 9.

Table 9 The effectiveness of the treatment of colibacillosis in calves Indicators Group of animals Control Experienced Number of animals, goal. 43 48 Recover, goal. 38 44 % 88.3 91.6 Remained sick, goal. 3 2 % 7.0 4.2 It’s a goal. 2 2 % 4.7 4.2 Duration of recovery, days 4.4 ± 0.5 4.3 ± 0.6

The fourth experiment was carried out in LLC "New Village" of the Lipetsk region of the Lipetsk region from February to March 2005.

For the treatment of calves in the control group (41 animals), enromag-o was used at a dose of 0.5 ml / 10 kg of body weight orally in a mixture with warm water for 3 days. The only source of water during the treatment was the treatment mixture.

For the treatment of calves of the experimental group (49 goals), a preparation was used to treat colibacteriosis in young farm animals in the following ratio of components, wt.%:

Norfloxacin - 2,

Dioxidine - 2,

Sodium chloride - 10,

Potassium chloride - 0.4,

Glucose - 36,

Polyvinylpyrrolidone low molecular weight - 49.6.

Before use, the drug was dissolved in warm (37-38 ° C) drinking water at the rate of 50 g of the drug per 1 liter of water. The calves were prescribed the drug orally at a dose of 10 ml / kg body weight daily for 3 days. The results of the experiment to study the effectiveness of the treatment of colibacillosis in calves are presented in table 10.

Table 10 The effectiveness of the treatment of colibacillosis in calves Indicators Group of animals Control Experienced Number of animals, goals 41 49 Recover, goal. 36 46 % 87.8 93.9 Remained sick, goal. four 3 % 9.8 6.1 It’s a goal. one - % 2,4 - Duration of recovery, days 4.0 ± 0.5 3.8 ± 0.5

Studies have shown that the drug for the treatment of colibacillosis in young farm animals has high therapeutic efficacy in the treatment of colibacillosis in calves. Compared with control groups, the use of the drug reduced the recovery period and death of animals.

Tests of the drug for the treatment of colibacteriosis in young farm animals have shown its high efficiency.

Based on the results of the studies, it was found that the drug has high antimicrobial activity, a pronounced therapeutic effect in gastrointestinal diseases of bacterial etiology in piglets and calves.

The use of the drug is technologically and cost-effective.

Claims (1)

A preparation for treating colibacteriosis in young farm animals, containing a preparation of the fluoroquinolone group, characterized in that it contains norfloxacin and additionally contains dioxidine, sodium chloride, potassium chloride, low molecular weight polyvinylpyrrolidone and glucose in the following ratio of components, wt.%:
Norfloxacin 1-3 Dioxidine 1-3 Sodium Chloride 10 Potassium chloride 0.4 Low molecular weight polyvinylpyrrolidone 49.6 Glucose Rest